##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931062_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2867951 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.08846315714599 33.0 33.0 33.0 27.0 33.0 2 31.010260286873798 33.0 33.0 33.0 27.0 33.0 3 31.03294024200553 33.0 33.0 33.0 27.0 33.0 4 34.64546256194754 37.0 37.0 37.0 33.0 37.0 5 34.633214793418716 37.0 37.0 37.0 33.0 37.0 6 34.718661859982966 37.0 37.0 37.0 33.0 37.0 7 34.69084234702755 37.0 37.0 37.0 33.0 37.0 8 34.68851420404324 37.0 37.0 37.0 33.0 37.0 9 34.71131131598831 37.0 37.0 37.0 33.0 37.0 10-11 34.60387381095423 37.0 37.0 37.0 33.0 37.0 12-13 34.521013783010936 37.0 37.0 37.0 33.0 37.0 14-15 36.155793805403235 40.0 37.0 40.0 33.0 40.0 16-17 36.11481385142215 40.0 37.0 40.0 33.0 40.0 18-19 36.056187152430425 40.0 37.0 40.0 33.0 40.0 20-21 35.9710926023492 40.0 37.0 40.0 27.0 40.0 22-23 35.894552417387885 40.0 37.0 40.0 27.0 40.0 24-25 35.778241678466614 40.0 37.0 40.0 27.0 40.0 26-27 35.620459868386874 40.0 37.0 40.0 27.0 40.0 28-29 35.516197975488424 40.0 37.0 40.0 27.0 40.0 30-31 35.38829185017457 40.0 37.0 40.0 27.0 40.0 32-33 35.24199332554845 37.0 37.0 40.0 27.0 40.0 34-35 35.140790585334265 37.0 37.0 40.0 27.0 40.0 36-37 34.949808242888395 37.0 37.0 40.0 27.0 40.0 38-39 34.78539591506271 37.0 37.0 40.0 27.0 40.0 40-41 34.59603546225162 37.0 37.0 40.0 22.0 40.0 42-43 34.45045400008577 37.0 37.0 40.0 22.0 40.0 44-45 34.228955271551015 37.0 33.0 40.0 22.0 40.0 46-47 34.11385916286575 37.0 33.0 40.0 22.0 40.0 48-49 34.14421271493133 37.0 33.0 40.0 22.0 40.0 50-51 33.40520619076128 37.0 33.0 38.5 22.0 40.0 52-53 33.53777243753467 37.0 33.0 38.5 22.0 40.0 54-55 34.071224020215126 37.0 33.0 40.0 22.0 40.0 56-57 34.06000573231551 37.0 33.0 40.0 22.0 40.0 58-59 33.94499383008984 37.0 33.0 40.0 22.0 40.0 60-61 33.97317649429854 37.0 33.0 40.0 22.0 40.0 62-63 33.823779590376546 37.0 33.0 40.0 18.5 40.0 64-65 33.634616316666495 37.0 33.0 40.0 15.0 40.0 66-67 33.419490953646 37.0 33.0 40.0 15.0 40.0 68-69 33.21788970592594 37.0 33.0 40.0 15.0 40.0 70-71 32.98942380814735 37.0 33.0 40.0 15.0 40.0 72-73 32.3971853075593 37.0 33.0 40.0 6.0 40.0 74-75 32.1994563365971 37.0 33.0 37.0 6.0 40.0 76-77 31.82055638328549 37.0 33.0 37.0 4.0 40.0 78-79 31.537464726559136 37.0 33.0 37.0 2.0 38.5 80-81 31.258834268786323 37.0 33.0 37.0 2.0 37.0 82-83 30.974959823232687 37.0 33.0 37.0 2.0 37.0 84-85 30.583427854938943 37.0 33.0 37.0 2.0 37.0 86-87 30.39084262597234 37.0 33.0 37.0 2.0 37.0 88-89 30.165967619390987 37.0 33.0 37.0 2.0 37.0 90-91 29.872711040042176 33.0 33.0 37.0 2.0 37.0 92-93 29.586318246023033 33.0 30.0 37.0 2.0 37.0 94-95 29.336201002039434 33.0 27.0 37.0 2.0 37.0 96-97 29.04211438758891 33.0 27.0 37.0 2.0 37.0 98-99 28.712191386812396 33.0 27.0 37.0 2.0 37.0 100-101 28.027394819507027 33.0 27.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23222088037219635 1101 2 0.22454144774882323 1101 3 0.12114360197630347 1101 4 0.28415978551163334 1101 5 0.21671615348208917 1101 6 0.28925842503127086 1101 7 0.19411012236648872 1101 8 0.2266678736852441 1101 9 0.31796425583443266 1101 10-11 0.29523229746691726 1101 12-13 0.37775995471882595 1101 14-15 0.4785510826475772 1101 16-17 0.5127009010797963 1101 18-19 0.5099015750611215 1101 20-21 0.5256519102514687 1101 22-23 0.5233695922706616 1101 24-25 0.5041628372313554 1101 26-27 0.5290126136930766 1101 28-29 0.5455054976177607 1101 30-31 0.4944964337674733 1101 32-33 0.5555863950131936 1101 34-35 0.5906184366147329 1101 36-37 0.587866788593324 1101 38-39 0.5937344092325816 1101 40-41 0.568074850547454 1101 42-43 0.6297637890619896 1101 44-45 0.6232405920200392 1101 46-47 0.6176190085101538 1101 48-49 0.7300493768276013 1101 50-51 0.6756774866270803 1101 52-53 0.7789952205002422 1101 54-55 0.7083093962929681 1101 56-57 0.7440912854044228 1101 58-59 0.781633880379573 1101 60-61 0.6787661622139893 1101 62-63 0.7520566090055851 1101 64-65 0.8113549655360259 1101 66-67 0.7937105528311292 1101 68-69 0.7408676199940771 1101 70-71 0.7060151160325105 1101 72-73 0.6305438674100827 1101 74-75 0.5847164800524922 1101 76-77 0.5941199498225025 1101 78-79 0.5587153578310655 1101 80-81 0.5054936707480309 1101 82-83 0.5213680826522626 1101 84-85 0.619767157886205 1101 86-87 0.5454426406175834 1101 88-89 0.43997405512060794 1101 90-91 0.43727147030046254 1101 92-93 0.6371505171160692 1101 94-95 0.5834018130383889 1101 96-97 0.3649834076647025 1101 98-99 0.522527665212035 1101 100-101 0.5420187797350025 1102 1 0.1694517441751806 1102 2 0.12706319432027868 1102 3 0.20999971747369273 1102 4 0.2227475725043817 1102 5 0.14677069236395823 1102 6 0.11764885566665129 1102 7 0.14484752367521025 1102 8 0.257271643382019 1102 9 0.23923703930825724 1102 10-11 0.2309196393120274 1102 12-13 0.19311859632868789 1102 14-15 0.3244080136959724 1102 16-17 0.34041374330124086 1102 18-19 0.34726079646313224 1102 20-21 0.35872975084006953 1102 22-23 0.32164602040175794 1102 24-25 0.3118132994060261 1102 26-27 0.39993918092189773 1102 28-29 0.388739305786892 1102 30-31 0.36285392801757865 1102 32-33 0.30483729307979246 1102 34-35 0.30295290333693004 1102 36-37 0.2934888514309151 1102 38-39 0.2693189602167436 1102 40-41 0.27504012763396446 1102 42-43 0.21116180414707486 1102 44-45 0.41055397142240224 1102 46-47 0.39560471977714684 1102 48-49 0.4204071015974833 1102 50-51 0.3686126669127461 1102 52-53 0.35304594183502047 1102 54-55 0.3949769710678197 1102 56-57 0.49109225812799906 1102 58-59 0.465621575513687 1102 60-61 0.5610793582483424 1102 62-63 0.5807028816580768 1102 64-65 0.5033289482365646 1102 66-67 0.49353268587247356 1102 68-69 0.3906471418052604 1102 70-71 0.4494115978118387 1102 72-73 0.31035409026331706 1102 74-75 0.3437137365179552 1102 76-77 0.4177968623943862 1102 78-79 0.3032401640285549 1102 80-81 0.31258695616119425 1102 82-83 0.3234652886478706 1102 84-85 0.45571881160359595 1102 86-87 0.422410048265597 1102 88-89 0.3753480123242632 1102 90-91 0.4162263250991991 1102 92-93 0.3355345211396852 1102 94-95 0.24641212181263583 1102 96-97 0.37829426532049126 1102 98-99 0.3712250538771009 1102 100-101 0.41625976719786806 1103 1 0.050458683834516904 1103 2 -0.0525119356894308 1103 3 -8.488586006549781E-5 1103 4 0.04299170404902242 1103 5 -0.08682289799496345 1103 6 0.07004288765465816 1103 7 0.0760888213804165 1103 8 0.05080244257543143 1103 9 -0.02741838070666347 1103 10-11 -0.037166639931250245 1103 12-13 0.07629067485250118 1103 14-15 0.1189530707925357 1103 16-17 0.13353895021587903 1103 18-19 0.12648498341733472 1103 20-21 0.0682153511657475 1103 22-23 0.050931984738781466 1103 24-25 0.06384522088855249 1103 26-27 0.12262827119449327 1103 28-29 0.1819094993085031 1103 30-31 0.2240211403844583 1103 32-33 0.08396406185338634 1103 34-35 0.1443149203843248 1103 36-37 0.051957342589624034 1103 38-39 -0.006628015780364649 1103 40-41 0.24177261097386804 1103 42-43 0.24276362832230092 1103 44-45 0.1687234758885907 1103 46-47 0.1467358794970295 1103 48-49 -0.08586282754861685 1103 50-51 -0.01044915640710542 1103 52-53 0.10894552349125064 1103 54-55 0.21814610328567596 1103 56-57 0.29041524137365826 1103 58-59 0.25333294524498484 1103 60-61 0.25377719239666163 1103 62-63 0.36294616099164756 1103 64-65 0.34681642406022206 1103 66-67 0.3341534535277404 1103 68-69 0.342086000412678 1103 70-71 0.27205316702072224 1103 72-73 0.20201002508976273 1103 74-75 0.1728418517216852 1103 76-77 0.1586992715539104 1103 78-79 0.30028942277916215 1103 80-81 0.2814757869687803 1103 82-83 0.3678656441054322 1103 84-85 0.385271923520456 1103 86-87 0.24952532961405538 1103 88-89 0.2875237008648739 1103 90-91 0.29219017107392986 1103 92-93 0.29053967717510076 1103 94-95 0.20500337571926508 1103 96-97 0.16117440610956635 1103 98-99 0.1841944233834667 1103 100-101 0.25616605566023054 1104 1 0.029639213700466627 1104 2 0.10095209533840688 1104 3 0.049711817845576434 1104 4 0.08928178787600416 1104 5 -0.0692762914580598 1104 6 -0.08881214761552769 1104 7 0.021432026873689836 1104 8 0.026449062619477104 1104 9 -0.006792696735232084 1104 10-11 -0.040575677588613246 1104 12-13 -0.06879437880394335 1104 14-15 0.12555641008922436 1104 16-17 0.07942064969019214 1104 18-19 0.030492608062083093 1104 20-21 0.16626673895584076 1104 22-23 0.09144748901007205 1104 24-25 0.11521082067464761 1104 26-27 0.1985127823599413 1104 28-29 0.06687756456179272 1104 30-31 0.12288929687988315 1104 32-33 0.15421761023515046 1104 34-35 0.15244456691064556 1104 36-37 0.1560338812324531 1104 38-39 0.18493911610173086 1104 40-41 0.17234561708516338 1104 42-43 0.09081997792025476 1104 44-45 0.02188001265696471 1104 46-47 0.16072418065671457 1104 48-49 0.20328376906467582 1104 50-51 0.20467071260116398 1104 52-53 0.21044247335769484 1104 54-55 0.2073669891889054 1104 56-57 0.34642633377078624 1104 58-59 0.3180511834724058 1104 60-61 0.39684375504913305 1104 62-63 0.2741803831317853 1104 64-65 0.26712717106039463 1104 66-67 0.4288087594921066 1104 68-69 0.43621438994767203 1104 70-71 0.3085489177836038 1104 72-73 0.317928193851877 1104 74-75 0.33815150988068865 1104 76-77 0.31511654305576897 1104 78-79 0.4198456993282811 1104 80-81 0.40298205400776865 1104 82-83 0.386930563848189 1104 84-85 0.40660521642424285 1104 86-87 0.4416031142219765 1104 88-89 0.46170989546146757 1104 90-91 0.47408825554044043 1104 92-93 0.5449170943335808 1104 94-95 0.5207689688896089 1104 96-97 0.4723827165864236 1104 98-99 0.4743518505206943 1104 100-101 0.4328230927442327 1105 1 0.09459487211298523 1105 2 0.091447071774585 1105 3 0.20500123307154539 1105 4 0.054006884892579876 1105 5 0.12435862905893202 1105 6 -0.01931807014590703 1105 7 0.14847556607779921 1105 8 0.08949410753066189 1105 9 0.15622411616828202 1105 10-11 0.13574905984133068 1105 12-13 0.14777062787020157 1105 14-15 0.14156073899706456 1105 16-17 0.19721151985515917 1105 18-19 0.17557045658497827 1105 20-21 0.21448304566440868 1105 22-23 0.1418374126598252 1105 24-25 0.1624340541310616 1105 26-27 0.22334790991165931 1105 28-29 0.14281733132578722 1105 30-31 0.09865533773704982 1105 32-33 0.20157085951634457 1105 34-35 0.13348664993750958 1105 36-37 0.12896792977664973 1105 38-39 0.19277721296722916 1105 40-41 0.1348334513187055 1105 42-43 0.13835995469076323 1105 44-45 0.1376432550782738 1105 46-47 0.1774798772072117 1105 48-49 0.16432421760688953 1105 50-51 0.15275353639629685 1105 52-53 0.2529624772210255 1105 54-55 0.320020624555184 1105 56-57 0.32785041567333195 1105 58-59 0.4144150742393009 1105 60-61 0.3974899956106057 1105 62-63 0.36646821587942924 1105 64-65 0.43349703017277363 1105 66-67 0.5045568457829859 1105 68-69 0.46303764519721824 1105 70-71 0.49026101569418756 1105 72-73 0.3320250048732589 1105 74-75 0.4341468986527701 1105 76-77 0.384458192448907 1105 78-79 0.45296543523518196 1105 80-81 0.3521064902962969 1105 82-83 0.23564749339447033 1105 84-85 0.3064838683211093 1105 86-87 0.4358260777407743 1105 88-89 0.3295509145874149 1105 90-91 0.3174484092810559 1105 92-93 0.410974791641884 1105 94-95 0.5450331412336347 1105 96-97 0.3947547252937653 1105 98-99 0.26434125113535956 1105 100-101 0.43973659135863485 1106 1 0.05298468676551593 1106 2 0.070808372512559 1106 3 0.07289151991160026 1106 4 0.06546547474908948 1106 5 0.022108028748476727 1106 6 -0.004430296955654001 1106 7 0.10611919871959685 1106 8 0.10193109064801575 1106 9 0.09634245516269146 1106 10-11 0.0910321246157011 1106 12-13 0.06258348704886885 1106 14-15 0.14426962373968166 1106 16-17 0.14670779153344427 1106 18-19 0.13703041456510334 1106 20-21 0.24216924543856067 1106 22-23 0.17368307796354543 1106 24-25 0.20849785158369372 1106 26-27 0.14792602866034343 1106 28-29 0.20216560900607305 1106 30-31 0.24345347294759279 1106 32-33 0.21061933324504167 1106 34-35 0.17615158463364367 1106 36-37 0.22015151939281452 1106 38-39 0.3371865422390883 1106 40-41 0.2833504792809265 1106 42-43 0.23970408067054194 1106 44-45 0.20415865164554248 1106 46-47 0.23630327611433444 1106 48-49 0.26428875732203494 1106 50-51 0.13535322268924688 1106 52-53 0.3579702163634906 1106 54-55 0.44756450249785473 1106 56-57 0.4048469332441016 1106 58-59 0.44352634114395073 1106 60-61 0.42322661924423954 1106 62-63 0.37000352123352087 1106 64-65 0.43979039876671777 1106 66-67 0.4731410301261789 1106 68-69 0.47275945759862736 1106 70-71 0.5557212931496522 1106 72-73 0.34394321030922015 1106 74-75 0.5553853906995343 1106 76-77 0.4299281503033008 1106 78-79 0.4778785963624266 1106 80-81 0.5851308495522467 1106 82-83 0.5676794453666751 1106 84-85 0.6124271821531373 1106 86-87 0.7487213083139714 1106 88-89 0.5829255578584274 1106 90-91 0.6065638027423113 1106 92-93 0.6942852736133425 1106 94-95 0.7231277074164311 1106 96-97 0.8118662170144013 1106 98-99 0.6778702927019147 1106 100-101 0.68254525298617 1107 1 0.0466373228025887 1107 2 0.12517042104702725 1107 3 0.14833096017914826 1107 4 0.21807610980983583 1107 5 0.11935239296054334 1107 6 0.2629275601887784 1107 7 0.12054684373456581 1107 8 0.05836762904091586 1107 9 0.15974378334493622 1107 10-11 0.06319175248378173 1107 12-13 0.15201530857306267 1107 14-15 0.18850847658715963 1107 16-17 0.20768122219193685 1107 18-19 0.2445045234049843 1107 20-21 0.22247960570418002 1107 22-23 0.13625330115596768 1107 24-25 0.19705125178534644 1107 26-27 0.15199494263193003 1107 28-29 0.1615760428503421 1107 30-31 0.3088372043368395 1107 32-33 0.27746946405087414 1107 34-35 0.22529564147555448 1107 36-37 0.2393938047089037 1107 38-39 0.2371668484141196 1107 40-41 0.30560188572113844 1107 42-43 0.14792678737170917 1107 44-45 0.1950946675243017 1107 46-47 0.07651195160462976 1107 48-49 0.08253317954601869 1107 50-51 0.07604644300281649 1107 52-53 0.1580727777116735 1107 54-55 0.3536618688149744 1107 56-57 0.3410328950345942 1107 58-59 0.480273286305021 1107 60-61 0.4177591995619707 1107 62-63 0.3857924699285462 1107 64-65 0.3963338348694947 1107 66-67 0.32767649825154166 1107 68-69 0.32027472816027114 1107 70-71 0.23080346604120194 1107 72-73 0.32941169024935846 1107 74-75 0.36149212727811175 1107 76-77 0.33115553829874145 1107 78-79 0.3426314859429702 1107 80-81 0.3097588030883074 1107 82-83 0.324655436454929 1107 84-85 0.4582674312858366 1107 86-87 0.35664527108357547 1107 88-89 0.3566244429949492 1107 90-91 0.29533481157186614 1107 92-93 0.42671582991971846 1107 94-95 0.4636084925094828 1107 96-97 0.42307256144870564 1107 98-99 0.3541634498185289 1107 100-101 0.45407630604428917 1108 1 -0.09843539591397032 1108 2 0.014140380378549366 1108 3 -0.05900676525256543 1108 4 -0.06395871543963949 1108 5 -0.09523591629633898 1108 6 -0.08663175093906972 1108 7 -0.14770824049188747 1108 8 -0.04846947842815297 1108 9 -0.03108976910771588 1108 10-11 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68-69 -1.2454749344725258 2316 70-71 -1.2200361886607354 2316 72-73 -1.0052083813570611 2316 74-75 -1.240614416395573 2316 76-77 -1.1069475138837355 2316 78-79 -1.1716419259045914 2316 80-81 -1.1704959736429572 2316 82-83 -1.1184334694434241 2316 84-85 -1.1163785126747712 2316 86-87 -1.0491453702944256 2316 88-89 -0.9747257332551449 2316 90-91 -0.9859770933812761 2316 92-93 -1.0137351490435655 2316 94-95 -0.9989879769567231 2316 96-97 -1.1047889959244657 2316 98-99 -1.0435896872842 2316 100-101 -1.1408570955160364 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 83568.0 3 12609.0 4 9389.0 5 7829.0 6 7781.0 7 8374.0 8 8626.0 9 8554.0 10 8975.0 11 9004.0 12 9297.0 13 9776.0 14 10349.0 15 11250.0 16 13213.0 17 15247.0 18 13592.0 19 11502.0 20 11966.0 21 12903.0 22 14473.0 23 16304.0 24 18929.0 25 21852.0 26 24707.0 27 29363.0 28 35008.0 29 41644.0 30 51678.0 31 64574.0 32 82724.0 33 109967.0 34 150986.0 35 218501.0 36 341428.0 37 651171.0 38 710324.0 39 514.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.51436171715069 24.335681013913486 13.553366060691014 22.596591208244813 2 13.507225677411617 22.31110094468706 44.63227920223947 19.54939417566186 3 16.135316402511204 32.14939617042865 31.060149340381354 20.65513808667879 4 10.42216933845899 18.905306101100972 44.40276506533469 26.269759495105344 5 10.92052978285096 39.098717803878245 37.541406719639376 12.439345693631418 6 24.098633484323827 42.64706754055421 21.831056388341363 11.423242586780596 7 22.962665680131913 33.515530774410024 28.18747600638923 15.334327539068834 8 22.217325191399713 38.34145004569464 23.11099457417508 16.33023018873056 9 25.502597499050715 16.091313972937474 24.44326977692436 33.96281875108745 10-11 21.86740038483941 28.400238567659574 31.97964259830935 17.75271844919166 12-13 22.108426246696464 26.656606052512434 31.58830028213537 19.646667418655735 14-15 19.966940483106207 28.30781719318042 28.786275331623813 22.938966992089565 16-17 18.705655226474242 30.358879816695627 30.370561251078136 20.56490370575199 18-19 18.59358047386725 30.97280747436606 31.469681677269058 18.96393037449764 20-21 19.14227599116512 29.518762614626716 30.879002270087994 20.459959124120168 22-23 19.32989789574508 30.43017471358472 29.94059173256447 20.299335658105736 24-25 19.14979340644014 30.342980177478683 30.68665771718475 19.820568698896423 26-27 19.38319378538894 29.864910523227213 30.392708940982605 20.359186750401246 28-29 19.218442016617438 30.861632573220394 29.69703805957633 20.222887350585836 30-31 20.479795505571747 30.147830280224454 29.250726389676814 20.12164782452699 32-33 20.23802010564337 30.40702229570868 29.37963375245951 19.975323846188445 34-35 20.40151662284328 29.5737793288658 30.21191784657409 19.812786201716836 36-37 20.53891436778383 29.997269827831786 30.351093864574395 19.11272193980999 38-39 20.8414299965376 29.394417826524933 30.833040731867456 18.931111445070016 40-41 19.969382263365883 29.50673225833615 30.777014416766292 19.746871061531678 42-43 20.567583116860476 29.46372844714866 29.917955333949337 20.05073310204153 44-45 19.70633871816955 30.85042173274801 29.94659911504116 19.496640434041275 46-47 20.733597572932887 29.58508577721607 29.457313986973176 20.224002662877872 48-49 20.48572946940655 28.7959474710329 30.28582412092967 20.432498938630875 50-51 20.502250515506137 29.28792422278546 29.077678925521045 21.132146336187358 52-53 19.46153233980517 29.846738034416386 29.826221482561365 20.865508143217077 54-55 20.06081988265904 30.271603029591283 29.25921501846755 20.40836206928213 56-57 20.44216581559016 29.485495449959163 29.641729632690566 20.4306091017601 58-59 20.426685026982742 28.78817018446453 30.40181957352828 20.383325215024456 60-61 20.31734084363658 30.380527236152936 29.126990175589228 20.175141744621257 62-63 20.576222535330913 29.664945151502597 28.66528127432324 21.09355103884325 64-65 19.703656006896306 29.588046403245123 29.03663557596109 21.671662013897482 66-67 20.151308002085294 28.300038570625404 29.83057761040732 21.718075816881978 68-69 19.52071304200444 29.32984570384679 29.136051622507793 22.013389631640976 70-71 20.06130688410147 28.755815564874553 29.78605501682086 21.396822534203114 72-73 20.684133225111648 28.545718391713308 29.980903088885846 20.7892452942892 74-75 20.31588477542197 29.504137053600605 29.15423839262138 21.025739778356044 76-77 19.97452353521289 29.193720575831943 30.17603595257793 20.65571993637724 78-79 19.594807575580003 29.05558888816976 29.922264067772296 21.42733946847794 80-81 19.741301594384232 29.084626085180698 30.48947742775195 20.684594892683123 82-83 20.417990579706093 28.181481906211015 30.676527721060847 20.723999793022045 84-85 21.25520814032595 28.35418570520615 29.43306346677033 20.95754268769757 86-87 20.11515374249663 29.319606596201535 29.733306560708588 20.831933100593243 88-89 20.766518895327245 28.482388285898487 29.703856728850397 21.047236089923874 90-91 20.594229165974838 27.756107195103407 30.736306547374564 20.91335709154719 92-93 20.496401761592423 28.633808486224105 29.779825406120846 21.089964346062622 94-95 19.43068522291336 28.386009984883824 30.26038069006952 21.922924102133294 96-97 19.982688828550792 28.401022818589013 29.432657605244888 22.183630747615307 98-99 19.38096207492691 29.02552559840298 29.800676087380857 21.792836239289258 100-101 19.958406891774118 27.879463786900267 29.707367762408097 22.454761558917518 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 41.0 1 28.0 2 35.0 3 47.5 4 36.5 5 32.0 6 41.5 7 89.5 8 143.0 9 163.5 10 174.0 11 197.5 12 289.0 13 403.0 14 500.0 15 640.0 16 794.5 17 945.0 18 1072.5 19 1181.0 20 1307.0 21 1472.5 22 1737.5 23 2046.5 24 2393.5 25 3051.0 26 4763.0 27 8252.5 28 16161.0 29 33399.5 30 56157.0 31 77861.0 32 103060.5 33 129952.0 34 162802.5 35 186924.5 36 198055.5 37 212981.5 38 203841.0 39 166300.5 40 133040.5 41 107997.0 42 89125.5 43 77084.5 44 67037.5 45 61905.0 46 59468.5 47 57759.0 48 57448.5 49 55730.0 50 53073.0 51 51632.0 52 49238.0 53 46963.5 54 45018.5 55 41338.5 56 37243.0 57 31951.5 58 26923.5 59 24426.5 60 21147.5 61 17207.5 62 14025.0 63 11671.5 64 9575.0 65 7251.5 66 5648.0 67 4621.0 68 3897.5 69 3309.0 70 2718.5 71 2215.5 72 1879.5 73 1575.5 74 1328.0 75 1137.5 76 954.0 77 746.5 78 588.5 79 497.0 80 420.0 81 348.0 82 287.5 83 237.0 84 194.0 85 155.0 86 117.5 87 102.5 88 85.5 89 63.5 90 48.0 91 43.0 92 34.5 93 20.5 94 14.5 95 11.0 96 6.0 97 3.5 98 4.5 99 3.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.8815338895260066E-4 2 0.004288776202940705 3 2.0920859526540027E-4 4 9.065705794834012E-4 5 0.0020572178534431027 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.2302148816350066E-5 12-13 5.2302148816350066E-5 14-15 0.003242733226613704 16-17 0.0053173851296622575 18-19 0.004271342153335256 20-21 1.7434049605450023E-5 22-23 0.0 24-25 3.4868099210900046E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.9177454565995025E-4 42-43 3.4868099210900046E-5 44-45 7.32230083428901E-4 46-47 0.69403208074336 48-49 0.49950295524574856 50-51 0.38529249628044554 52-53 0.3236979990243906 54-55 0.31442308463429114 56-57 0.28387862972554273 58-59 0.28452368956094437 60-61 0.26238244656202286 62-63 0.2576578191189459 64-65 0.25415357514825043 66-67 0.24287374505352427 68-69 0.22345221379305294 70-71 0.23610933380660967 72-73 0.7650409647863579 74-75 0.502466743678675 76-77 0.5957040409686218 78-79 0.33319955605936086 80-81 0.2890216743591505 82-83 0.26998369218999907 84-85 0.26238244656202286 86-87 0.23778300256873286 88-89 0.24011916521586318 90-91 0.25056216092952777 92-93 0.20903425476934576 94-95 0.1786467063070464 96-97 0.13614249336895923 98-99 0.14365656874890817 100-101 0.13999541833176368 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2867951.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.28497930588178 #Duplication Level Percentage of deduplicated Percentage of total 1 81.1292624909456 35.11678448022031 2 10.589265730284001 9.16712295999652 3 3.3832257205633023 4.393285659051284 4 1.5300683385311813 2.6491590547962836 5 0.8092825534265343 1.751488928883935 6 0.4823559043828267 1.2527259203568326 7 0.30865486454240254 0.9352083597064345 8 0.22927601222495195 0.7939365955593713 9 0.1749774082189164 0.6816504144377361 >10 1.0652308826079582 8.966971481895454 >50 0.13926469522932106 4.24660355233183 >100 0.1304124986706527 11.354334553107801 >500 0.01610100612429571 4.89260073937323 >1k 0.011408250570480671 9.439152117207152 >5k 9.709149421685679E-4 3.0496891410617977 >10k+ 2.4272873554214198E-4 1.3092860420141856 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 15227 0.5309365466843751 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 12043 0.4199165187968693 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 10115 0.352690823518254 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 9978 0.3479138939263607 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 9315 0.32479634414953396 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 9050 0.3155562978586454 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 7989 0.2785612445958805 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 7509 0.26182455697464846 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 7188 0.25063189712794953 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 6369 0.2220749238742224 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 6347 0.2213078256915826 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 6112 0.2131138223770211 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 5877 0.2049198190624596 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 5846 0.20383890798692164 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 5500 0.19177454565995025 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 4800 0.16736687621232024 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 4710 0.16422874728333922 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 4477 0.1561044801671995 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 4426 0.1543262071074436 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 4188 0.14602759949524938 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 4081 0.1422967128796831 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 3920 0.1366829489067282 No Hit GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG 3619 0.12618765104424726 No Hit CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA 3555 0.12395609269474966 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 3480 0.12134098525393217 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 3465 0.12081796376576866 No Hit GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG 3379 0.11781930723363127 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 3379 0.11781930723363127 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 3368 0.11743575814231136 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 3328 0.11604103417387536 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 3325 0.11593642987624267 No Hit GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT 3280 0.11436736541175214 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 3213 0.11203120276462185 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 3169 0.11049700639934226 No Hit AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA 3073 0.10714966887509585 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 3053 0.10645230689087784 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 2895 0.10094314721555563 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 3.4868099210900046E-5 6 0.0 0.0 0.0 0.0 3.4868099210900046E-5 7 0.0 0.0 0.0 0.0 3.4868099210900046E-5 8 0.0 0.0 0.0 0.0 3.4868099210900046E-5 9 0.0 0.0 0.0 0.0 3.4868099210900046E-5 10-11 0.0 0.0 0.0 0.0 3.4868099210900046E-5 12-13 0.0 0.0 0.0 0.0 3.4868099210900046E-5 14-15 0.0 0.0 0.0 0.0 3.4868099210900046E-5 16-17 0.0 0.0 0.0 0.0 6.973619842180009E-5 18-19 0.0 0.0 0.0 0.0 8.717024802725013E-5 20-21 0.0 0.0 0.0 3.4868099210900046E-5 1.3947239684360019E-4 22-23 0.0 0.0 0.0 1.9177454565995025E-4 1.7434049605450023E-4 24-25 0.0 0.0 0.0 2.9637884329265036E-4 1.7434049605450023E-4 26-27 0.0 0.0 0.0 6.97361984218001E-4 1.9177454565995025E-4 28-29 0.0 0.0 0.0 0.0027545798376611037 2.092085952654003E-4 30-31 0.0 0.0 0.0 0.011279830094726166 2.092085952654003E-4 32-33 0.0 0.0 0.0 0.03675097656828865 2.092085952654003E-4 34-35 0.0 0.0 0.0 0.08122523711179166 2.092085952654003E-4 36-37 0.0 0.0 0.0 0.1530709555358512 2.092085952654003E-4 38-39 0.0 0.0 0.0 0.2552170521741829 2.092085952654003E-4 40-41 0.0 0.0 3.4868099210900046E-5 0.361564754767428 2.092085952654003E-4 42-43 0.0 0.0 3.4868099210900046E-5 0.4767166524114254 2.092085952654003E-4 44-45 0.0 0.0 3.4868099210900046E-5 0.616084444957393 2.266426448708503E-4 46-47 0.0 0.0 3.4868099210900046E-5 0.7864674117514561 2.4407669447630033E-4 48-49 0.0 0.0 3.4868099210900046E-5 0.9626733511137394 2.4407669447630033E-4 50-51 0.0 0.0 3.4868099210900046E-5 1.1406749975853843 2.7894479368720037E-4 52-53 0.0 0.0 3.4868099210900046E-5 1.3184151333129472 2.7894479368720037E-4 54-55 0.0 0.0 3.4868099210900046E-5 1.5110090793043536 2.7894479368720037E-4 56-57 0.0 0.0 3.4868099210900046E-5 1.7445033056701456 2.9637884329265036E-4 58-59 0.0 0.0 3.4868099210900046E-5 2.016544215713588 3.138128928981004E-4 60-61 0.0 0.0 3.4868099210900046E-5 2.2921765399757525 3.138128928981004E-4 62-63 0.0 0.0 3.4868099210900046E-5 2.5647404715073585 3.138128928981004E-4 64-65 0.0 0.0 3.4868099210900046E-5 2.8499963911517314 3.138128928981004E-4 66-67 0.0 0.0 3.4868099210900046E-5 3.1700681078581887 3.138128928981004E-4 68-69 0.0 0.0 3.4868099210900046E-5 3.520597109225367 3.138128928981004E-4 70-71 0.0 0.0 3.4868099210900046E-5 3.890844020696309 3.138128928981004E-4 72-73 0.0 0.0 3.4868099210900046E-5 4.294302796665633 3.3124694250355046E-4 74-75 0.0 0.0 3.4868099210900046E-5 4.67208121756613 3.4868099210900045E-4 76-77 0.0 0.0 3.4868099210900046E-5 5.0983960325682 3.4868099210900045E-4 78-79 0.0 0.0 3.4868099210900046E-5 5.564844029762014 3.4868099210900045E-4 80-81 0.0 0.0 3.4868099210900046E-5 6.097628585704568 3.4868099210900045E-4 82-83 0.0 0.0 3.4868099210900046E-5 6.656808292749771 3.4868099210900045E-4 84-85 0.0 0.0 3.4868099210900046E-5 7.189871793486011 3.4868099210900045E-4 86-87 0.0 0.0 3.4868099210900046E-5 7.7696236790656465 3.4868099210900045E-4 88-89 0.0 0.0 3.4868099210900046E-5 8.382343352449187 3.4868099210900045E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3775 0.0 59.735405 2 ACCTGGG 3810 0.0 59.556564 3 GTACCTG 4365 0.0 51.986805 1 CCTGGGG 3270 0.0 51.57288 4 GTACCCG 270 0.0 49.12879 1 CTGGGGG 2880 0.0 47.20641 5 GTACACG 370 0.0 42.25266 1 TGGGGGG 5440 0.0 36.74663 6 GGGGGGA 5590 0.0 29.066067 7 TAACGCA 250 0.0 28.423029 4 ACACGGG 560 0.0 27.915476 3 GGGGAAT 6035 0.0 25.982742 9 ATGGGGG 4030 0.0 25.977583 5 ATAACGC 275 0.0 25.839117 3 CATGGGG 4425 0.0 25.80019 4 GTATCAA 5440 0.0 24.906284 1 GGGGGAA 6455 0.0 24.363846 8 GTACATG 6845 0.0 24.292685 1 TATTCGC 415 0.0 23.972067 9 TATATCC 5065 0.0 23.288366 4 >>END_MODULE