FastQCFastQC Report
Sat 18 Jun 2016
SRR1931061_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931061_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056226
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA17070.16161313961216633No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17060.16151846290471925No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA15600.14769566361744552No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC14250.13491430811208963No Hit
GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA13540.12819226188334693No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC12900.12213295260673379No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC12500.11834588430885058No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC12370.11711508711203852No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11880.11247592844713158No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11360.1075527396598834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG21800.063.238422
GTACCTG23150.061.187781
ACCTGGG23700.058.3685653
CCTGGGG20150.050.5460664
GTACCCG2300.049.4342421
CTGGGGG17950.045.9207235
TAACGCA2100.036.0931364
ATAACGC2000.035.530863
GTACACG2700.035.0922131
TGGGGGG27450.032.0961846
GTATAAC3250.032.0688861
GGGGAAT24350.030.7370019
GGGGGGA27200.029.7789967
TACACGG3100.029.0359762
GTATCAA31950.028.3208961
TACCGGG1205.4929933E-627.6351172
GGGGGAA29000.026.461868
CAACGCA34450.025.9894165
ATCAACG34500.025.6783453
GGTATCA21700.025.5429251
Produced by FastQC (version 0.11.5)