##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931061_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1056226 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.315847176645907 33.0 33.0 33.0 27.0 33.0 2 30.174869772188906 33.0 33.0 33.0 27.0 33.0 3 30.235655058671156 33.0 33.0 33.0 27.0 33.0 4 33.720802176806856 37.0 37.0 37.0 27.0 37.0 5 33.6596836283144 37.0 37.0 37.0 27.0 37.0 6 33.76455701715353 37.0 37.0 37.0 27.0 37.0 7 33.738519029071426 37.0 37.0 37.0 27.0 37.0 8 33.72281500360718 37.0 37.0 37.0 27.0 37.0 9 33.77307886759084 37.0 37.0 37.0 27.0 37.0 10-11 33.62589729849483 37.0 37.0 37.0 27.0 37.0 12-13 33.49957253466587 37.0 37.0 37.0 27.0 37.0 14-15 34.921159865407596 38.5 37.0 40.0 27.0 40.0 16-17 34.84280873600915 37.0 37.0 40.0 27.0 40.0 18-19 34.75775165542223 37.0 37.0 40.0 24.5 40.0 20-21 34.6497761842636 37.0 37.0 40.0 22.0 40.0 22-23 34.515867342784595 37.0 37.0 40.0 22.0 40.0 24-25 34.36928507724673 37.0 37.0 40.0 22.0 40.0 26-27 34.169423021209475 37.0 33.0 40.0 15.0 40.0 28-29 34.00668370216223 37.0 33.0 40.0 15.0 40.0 30-31 33.82088539763271 37.0 33.0 40.0 15.0 40.0 32-33 33.616660638916294 37.0 33.0 40.0 15.0 40.0 34-35 33.437863203518944 37.0 33.0 40.0 15.0 40.0 36-37 33.16639336657117 37.0 33.0 40.0 10.5 40.0 38-39 32.93442170520324 37.0 33.0 40.0 6.0 40.0 40-41 32.68911056913956 37.0 33.0 40.0 6.0 40.0 42-43 32.44272011861098 37.0 33.0 40.0 6.0 40.0 44-45 32.1101833319763 37.0 33.0 40.0 4.0 40.0 46-47 31.922705936040202 37.0 33.0 40.0 2.0 40.0 48-49 31.84985836364566 37.0 33.0 40.0 2.0 40.0 50-51 31.08780885908887 37.0 33.0 38.5 2.0 40.0 52-53 31.223755143312133 37.0 33.0 38.5 2.0 40.0 54-55 31.64144984122716 37.0 33.0 40.0 2.0 40.0 56-57 31.559333419173548 37.0 33.0 40.0 2.0 40.0 58-59 31.36960603128497 37.0 33.0 40.0 2.0 40.0 60-61 31.35020204009369 37.0 33.0 40.0 2.0 40.0 62-63 31.138897830577925 37.0 33.0 40.0 2.0 40.0 64-65 30.89162262621825 37.0 33.0 40.0 2.0 40.0 66-67 30.610203214084866 37.0 33.0 37.0 2.0 40.0 68-69 30.326315106804792 37.0 27.0 37.0 2.0 40.0 70-71 30.05610399668253 37.0 27.0 37.0 2.0 40.0 72-73 29.467340796382594 37.0 27.0 37.0 2.0 40.0 74-75 29.284241724782383 37.0 27.0 37.0 2.0 40.0 76-77 28.944977211316516 35.0 27.0 37.0 2.0 37.0 78-79 28.677364503430137 33.0 27.0 37.0 2.0 37.0 80-81 28.439662060960437 33.0 27.0 37.0 2.0 37.0 82-83 28.15412941927201 33.0 27.0 37.0 2.0 37.0 84-85 27.755541901070416 33.0 27.0 37.0 2.0 37.0 86-87 27.56893931791113 33.0 27.0 37.0 2.0 37.0 88-89 27.360408662540024 33.0 27.0 37.0 2.0 37.0 90-91 27.0496986440402 33.0 24.5 37.0 2.0 37.0 92-93 26.746560395218445 33.0 22.0 37.0 2.0 37.0 94-95 26.451050722099247 33.0 22.0 37.0 2.0 37.0 96-97 26.126302514802703 33.0 18.5 37.0 2.0 37.0 98-99 25.757900771236457 33.0 15.0 37.0 2.0 37.0 100-101 25.054856631061913 33.0 2.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06070190319677238 1101 2 0.2326575382115621 1101 3 0.3049574155162311 1101 4 0.4970918692431425 1101 5 0.39196052670332193 1101 6 0.4153626977425162 1101 7 0.38230744491573887 1101 8 0.3797866638271046 1101 9 0.16875999392426877 1101 10-11 0.32570361478000365 1101 12-13 0.2969684749511643 1101 14-15 0.44707896205992625 1101 16-17 0.20485980844650697 1101 18-19 0.28549296481127584 1101 20-21 0.30062633906063496 1101 22-23 0.06551387105904638 1101 24-25 0.21984795405570168 1101 26-27 0.3853406128928256 1101 28-29 0.293133877504971 1101 30-31 0.14196283816149702 1101 32-33 0.2136983046067087 1101 34-35 0.24754907624049594 1101 36-37 -0.025021982193152326 1101 38-39 0.20794394858538823 1101 40-41 0.27425483629774305 1101 42-43 0.09376947730681451 1101 44-45 0.08445148478280373 1101 46-47 0.1980968604362232 1101 48-49 0.2629185127298399 1101 50-51 0.05626415258403128 1101 52-53 0.1829919966071749 1101 54-55 0.27205484973266536 1101 56-57 0.3186865568104551 1101 58-59 0.5248713695742602 1101 60-61 0.48153846977124104 1101 62-63 0.41560100251585297 1101 64-65 0.3228533944493819 1101 66-67 0.36368709002855937 1101 68-69 0.4230403601835526 1101 70-71 0.34593611053094975 1101 72-73 0.4777940034646555 1101 74-75 0.23805628633110487 1101 76-77 0.2071415562807566 1101 78-79 0.265201622065419 1101 80-81 0.6586210682671378 1101 82-83 0.6407443425441421 1101 84-85 0.7764379678835525 1101 86-87 0.5439092667196803 1101 88-89 0.3825157435903037 1101 90-91 0.5229371085077474 1101 92-93 0.34182318292288727 1101 94-95 0.127251094195465 1101 96-97 0.3296068611443026 1101 98-99 0.4570728245384963 1101 100-101 0.6968477827948014 1102 1 0.019186602124200647 1102 2 0.17036907921444566 1102 3 -0.02227140071717315 1102 4 0.02590189969156853 1102 5 0.08305739798660028 1102 6 0.0630078671236518 1102 7 -0.08000690467742544 1102 8 0.012676890396789986 1102 9 0.030126639824523238 1102 10-11 0.049741549897085235 1102 12-13 0.06405626834420985 1102 14-15 0.09286189638141451 1102 16-17 0.13474686072124342 1102 18-19 0.18610071958417507 1102 20-21 0.3309048517537647 1102 22-23 0.17483151900626837 1102 24-25 0.24701487558292712 1102 26-27 0.2793826743236565 1102 28-29 0.19407605247685922 1102 30-31 0.3566848783404666 1102 32-33 0.08952053330158094 1102 34-35 0.10637048776342084 1102 36-37 -0.00597351553673775 1102 38-39 0.048596769177045473 1102 40-41 0.13703602570094375 1102 42-43 0.2618385512595296 1102 44-45 0.16771267504901743 1102 46-47 -0.08112849832382807 1102 48-49 0.2058398619535211 1102 50-51 -0.075659222039409 1102 52-53 -0.02195499509435095 1102 54-55 -0.09468814025360572 1102 56-57 -0.00295083460411405 1102 58-59 0.06046284833183435 1102 60-61 0.07805645429621322 1102 62-63 -0.050828873074440395 1102 64-65 -0.13908473241993136 1102 66-67 -0.16909148334566027 1102 68-69 -0.15016260321091934 1102 70-71 -0.2501604775739388 1102 72-73 -0.255521395237043 1102 74-75 -0.14887395199722775 1102 76-77 -0.1612984813684868 1102 78-79 -0.3112700290803758 1102 80-81 -0.25611054603849226 1102 82-83 -0.2767880409279435 1102 84-85 -0.03427413138530966 1102 86-87 -0.2501843062095439 1102 88-89 -0.31421300656813855 1102 90-91 -0.22771622450195395 1102 92-93 -0.28673036880840996 1102 94-95 -0.15330924842342597 1102 96-97 0.06986147897483264 1102 98-99 0.12469488116878225 1102 100-101 -0.18259036430154296 1103 1 0.02160062992713918 1103 2 -0.05095271118134548 1103 3 -0.04150772571484751 1103 4 -0.09824915087570929 1103 5 -0.13992434256172004 1103 6 0.054548976597118326 1103 7 -0.023551461363013004 1103 8 -0.09374109386551055 1103 9 -0.1869546879397319 1103 10-11 -0.07195017924213687 1103 12-13 -0.04820214525269506 1103 14-15 -0.022421396881334488 1103 16-17 -0.11265548867133646 1103 18-19 -0.030827589176347203 1103 20-21 -0.08675745856387351 1103 22-23 0.071992955279363 1103 24-25 -0.08126230181629523 1103 26-27 -0.22033454655743867 1103 28-29 -0.17507066459222642 1103 30-31 0.11642423189025664 1103 32-33 -0.08752857478124554 1103 34-35 -0.2613415231266245 1103 36-37 -0.30733771055884773 1103 38-39 -0.13521945132701063 1103 40-41 0.04272735331829125 1103 42-43 -0.1716300100680357 1103 44-45 -0.02287065787626119 1103 46-47 -0.40240475026411104 1103 48-49 -0.22472133830305552 1103 50-51 -0.17488041651799335 1103 52-53 -0.07934936301569095 1103 54-55 0.03996368642605219 1103 56-57 0.3198262277248105 1103 58-59 0.28118847668907065 1103 60-61 0.43786166722521713 1103 62-63 0.36413050956120685 1103 64-65 0.3251651316991939 1103 66-67 0.2941166273960292 1103 68-69 -0.0011575939808139424 1103 70-71 0.22285553923122237 1103 72-73 -0.10457977749565117 1103 74-75 -0.11750872868741169 1103 76-77 -0.009716632115466695 1103 78-79 0.22896539983801745 1103 80-81 0.0140830658465525 1103 82-83 0.1308974303461028 1103 84-85 0.11127509666442137 1103 86-87 0.23715184104125697 1103 88-89 -0.011893972962717214 1103 90-91 -0.05245805083884747 1103 92-93 0.11900747658608424 1103 94-95 0.11924942518336934 1103 96-97 -0.15474226301309457 1103 98-99 -0.10800050573670816 1103 100-101 -0.23786572505914805 1104 1 0.04850546965698754 1104 2 0.23484010711971948 1104 3 0.17146415961908446 1104 4 0.11028033704937457 1104 5 0.11708309893150215 1104 6 0.011782676840354611 1104 7 0.23286167947350833 1104 8 0.346119924680913 1104 9 0.20635348666579745 1104 10-11 0.2727473853986737 1104 12-13 0.1343893027608587 1104 14-15 0.13108752399602253 1104 16-17 0.30983178692710567 1104 18-19 0.08148356117747113 1104 20-21 0.19980264433504402 1104 22-23 0.3941962400639838 1104 24-25 0.33205151864547844 1104 26-27 0.2948604477330292 1104 28-29 0.2515873281148515 1104 30-31 0.26512250120185143 1104 32-33 0.2962898918381569 1104 34-35 0.48248093379718426 1104 36-37 0.5669592105392525 1104 38-39 0.4978497719644821 1104 40-41 0.6825471953192235 1104 42-43 0.6142116257869716 1104 44-45 0.49496791308536103 1104 46-47 0.33326942015584393 1104 48-49 0.20016118119212933 1104 50-51 0.3846706317859159 1104 52-53 0.40452917042008707 1104 54-55 0.6023726841379933 1104 56-57 0.6676205232875034 1104 58-59 0.6822618261251172 1104 60-61 0.7040266450875201 1104 62-63 0.7484912755298545 1104 64-65 0.6584043674285525 1104 66-67 0.6637143283014879 1104 68-69 0.7224193743217384 1104 70-71 0.8343803024660836 1104 72-73 0.527661669881148 1104 74-75 0.5969117644972322 1104 76-77 0.3892872775338567 1104 78-79 0.5587343442326969 1104 80-81 0.6634403721363782 1104 82-83 0.7309988859582113 1104 84-85 0.6339338704523669 1104 86-87 0.7416632197763313 1104 88-89 0.6423641912425495 1104 90-91 0.8986692101567719 1104 92-93 0.8544492329992259 1104 94-95 1.0329452960673038 1104 96-97 0.8081330484641249 1104 98-99 0.9002401096191726 1104 100-101 0.8569538834665202 1105 1 0.23618677642757646 1105 2 0.24028840472492874 1105 3 0.08968839330357525 1105 4 0.25434261609996867 1105 5 0.06502345401760579 1105 6 0.01967949031848093 1105 7 -0.004658351718248355 1105 8 0.10294452139869037 1105 9 0.06396086224854258 1105 10-11 0.12947189611524124 1105 12-13 0.3055993266583741 1105 14-15 0.5009631300619972 1105 16-17 0.2546067939695007 1105 18-19 0.2353860757333237 1105 20-21 0.4437028083156491 1105 22-23 0.46756543521583893 1105 24-25 0.4829813017396205 1105 26-27 0.31550166973366345 1105 28-29 0.38843884451571853 1105 30-31 0.3854341762851945 1105 32-33 0.23359949058004048 1105 34-35 0.24077184173484767 1105 36-37 0.27371562191977006 1105 38-39 0.37679281528782127 1105 40-41 0.3146318011035092 1105 42-43 0.24087265231676014 1105 44-45 0.16620312171757234 1105 46-47 -0.13107878958262376 1105 48-49 0.0966533207566016 1105 50-51 0.14833568227664173 1105 52-53 0.465465035161273 1105 54-55 0.564995273166037 1105 56-57 0.5242410303774747 1105 58-59 0.3898101574965338 1105 60-61 0.38148528149270433 1105 62-63 0.4388990630229266 1105 64-65 0.6038253162774225 1105 66-67 0.6093269609644096 1105 68-69 0.596444398620342 1105 70-71 0.564297342084469 1105 72-73 0.2379621330937134 1105 74-75 0.35196690041085077 1105 76-77 0.503637166254638 1105 78-79 0.5090038208939092 1105 80-81 0.41023351591882573 1105 82-83 0.4622469854435103 1105 84-85 0.6445207867028628 1105 86-87 0.4483725263752909 1105 88-89 0.3013022535850318 1105 90-91 0.24414453937722058 1105 92-93 0.18596102598443665 1105 94-95 0.05183991007859845 1105 96-97 0.17145829159009196 1105 98-99 0.14597549454452263 1105 100-101 -0.13760341553597755 1106 1 0.19366838988078428 1106 2 0.04896607854867696 1106 3 0.04191697814770379 1106 4 -0.026054944162183347 1106 5 0.03963113353079706 1106 6 0.2531576314146946 1106 7 0.17932450233578123 1106 8 0.08367426543069456 1106 9 0.14200937490021204 1106 10-11 0.019572933373027013 1106 12-13 0.09141274142989886 1106 14-15 0.17820243585565976 1106 16-17 0.3210482812515707 1106 18-19 0.3462724071616563 1106 20-21 0.3176713015039425 1106 22-23 0.3201786489163112 1106 24-25 0.29110186485217326 1106 26-27 0.2891489604337494 1106 28-29 0.3106421440121778 1106 30-31 0.18258529102727294 1106 32-33 0.30410310540969476 1106 34-35 0.26006718425144015 1106 36-37 0.2435081040964846 1106 38-39 0.002091605548180553 1106 40-41 0.1328082835528548 1106 42-43 0.18523258086268868 1106 44-45 0.15555175185001247 1106 46-47 -0.03757838299120664 1106 48-49 0.12070611421478361 1106 50-51 0.2054889932750612 1106 52-53 0.16237882168704942 1106 54-55 0.22490876717318997 1106 56-57 0.2388469869654415 1106 58-59 0.2037678398023779 1106 60-61 0.16421967742281396 1106 62-63 0.23793308890278198 1106 64-65 0.2967986213497298 1106 66-67 0.33907146630027185 1106 68-69 0.4238139178011373 1106 70-71 0.4348531180019499 1106 72-73 0.20083546116991613 1106 74-75 0.28208618931606466 1106 76-77 0.3010973662086336 1106 78-79 0.5578336044017291 1106 80-81 0.5851075861782036 1106 82-83 0.7215211984595449 1106 84-85 0.42682492465805666 1106 86-87 0.5993604157256236 1106 88-89 0.4393265506173414 1106 90-91 0.5636236290289709 1106 92-93 0.6939858815474516 1106 94-95 0.6806224172756004 1106 96-97 0.5618414341867677 1106 98-99 0.3364221581755622 1106 100-101 0.3283157982042546 1107 1 0.02069793867493175 1107 2 -0.14711133447449498 1107 3 -0.10352838492396188 1107 4 -0.20236182395672841 1107 5 -0.20720369176338238 1107 6 -0.08107084857613245 1107 7 -0.12377561828068195 1107 8 -0.2940603120938121 1107 9 -0.03547859703295586 1107 10-11 -0.04556577555626262 1107 12-13 -0.01240158622959342 1107 14-15 -0.1423834897167282 1107 16-17 -0.06222507218151918 1107 18-19 0.011938264644392405 1107 20-21 -0.15450184534996225 1107 22-23 -0.24998126863110315 1107 24-25 -0.1496702595824715 1107 26-27 -0.268116547109031 1107 28-29 -0.08593797191214492 1107 30-31 -0.030489013905814488 1107 32-33 -0.03259545999765123 1107 34-35 -0.07177538216595991 1107 36-37 -0.06356510660264547 1107 38-39 -0.28174883329156586 1107 40-41 -0.173715123830803 1107 42-43 0.027011454267345414 1107 44-45 0.09081465006565992 1107 46-47 -0.15440084218801076 1107 48-49 0.004458083074784014 1107 50-51 0.0639567254617468 1107 52-53 0.3186890683553223 1107 54-55 0.4334959965137024 1107 56-57 0.26337770655410964 1107 58-59 0.2860417869155931 1107 60-61 0.4154753153692745 1107 62-63 0.3650522717553635 1107 64-65 0.33901259154752594 1107 66-67 0.5168334444460392 1107 68-69 0.4475185892488831 1107 70-71 0.49666927774220326 1107 72-73 0.29958336023175747 1107 74-75 0.40319693528454437 1107 76-77 0.37727825687209204 1107 78-79 0.5528379159700734 1107 80-81 0.655695078779587 1107 82-83 0.5995790316793084 1107 84-85 0.7397413626131097 1107 86-87 0.7371850929400345 1107 88-89 0.5445838881186731 1107 90-91 0.5032383906211813 1107 92-93 0.2873637961094495 1107 94-95 0.24031861064877091 1107 96-97 0.25375144553205686 1107 98-99 0.4208308465006567 1107 100-101 0.40299860914982233 1108 1 -0.17435992191065708 1108 2 -0.29040162592417573 1108 3 -0.21463150166102452 1108 4 -0.3624030227895858 1108 5 -0.2817471456614342 1108 6 -0.2842832313749213 1108 7 -0.16589727996693426 1108 8 -0.27746816255174167 1108 9 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-0.4978024114929447 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49386.0 3 6692.0 4 5170.0 5 4268.0 6 4437.0 7 4626.0 8 4985.0 9 5216.0 10 5026.0 11 4986.0 12 5603.0 13 6063.0 14 6375.0 15 6914.0 16 7907.0 17 9746.0 18 8420.0 19 6750.0 20 6799.0 21 6915.0 22 7310.0 23 8255.0 24 9343.0 25 10702.0 26 11995.0 27 14225.0 28 16464.0 29 19884.0 30 23652.0 31 29818.0 32 37087.0 33 48287.0 34 64648.0 35 92113.0 36 137791.0 37 212369.0 38 145797.0 39 202.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.176401975338564 19.43167358439119 11.636736146877935 23.755188293392308 2 15.478428988833407 20.599970081027394 41.076950461377066 22.844650468762133 3 18.493881511988448 28.71244289805676 28.731567611067714 24.062107978887074 4 12.36622054579745 16.93659251516735 39.43909200390829 31.258094935126906 5 12.800581706908337 37.295990729046146 34.59773793270605 15.305689631339458 6 28.295838201294043 37.901358232045034 20.16273032476004 13.640073241900883 7 26.664653208688293 31.59314389155351 24.279936301511228 17.462266598246966 8 25.905914075207388 34.428427249471234 21.430640790891342 18.23501788443004 9 27.168522645721655 15.381651275389926 21.572655852061963 35.87717022682646 10-11 25.056013133559073 26.039751927973715 28.903344976995914 20.000889961471298 12-13 25.392897059289954 24.432069129242883 28.577447313520942 21.59758649794622 14-15 22.355884622485643 25.695545761395966 26.87971330780119 25.0688563083172 16-17 21.57245647148604 27.596976070738243 27.791261963565717 23.039305494210005 18-19 21.397212052009632 27.690403895514915 29.46527562775223 21.447108424723226 20-21 22.508298413407736 27.111337914423615 28.782855184401825 21.597508487766824 22-23 22.351040402338135 27.1605698022961 28.314584189368563 22.173805605997202 24-25 22.058452480081193 26.98277971891419 29.17857029583171 21.7801975051729 26-27 22.041494907339906 27.227364219400013 28.52391438953406 22.20722648372602 28-29 21.70790152865012 27.444315894514997 28.90167445224791 21.94610812458697 30-31 22.477244453365095 27.25458377279105 28.481641239658938 21.78653053418492 32-33 22.50190773565506 27.444221217807552 28.074815427758832 21.979055618778556 34-35 22.373384105295646 27.143906701785415 28.685622205853672 21.797086987065267 36-37 22.472510617992743 27.04108779749788 28.458208754565785 22.028192829943592 38-39 22.485244635144372 27.19976595917919 28.528695563260136 21.786293842416303 40-41 22.36544744812412 26.672729149052802 28.666010870810428 22.29581253201265 42-43 22.40779908845266 26.90338052651611 28.16532636007824 22.523494024952992 44-45 22.342536132438507 27.65592536014092 28.009024647835652 21.992513859584925 46-47 22.476925826630918 27.16644623175454 27.754828716115583 22.601799225498958 48-49 22.770242049269932 26.836966625343578 28.00972725811009 22.383064067276397 50-51 22.309801331426318 27.453611529524686 27.560524777739236 22.67606236130976 52-53 22.182262339348526 27.598881714261562 27.508271505796344 22.710584440593564 54-55 22.259540708781103 27.541375480377244 27.50390926051939 22.69517455032226 56-57 22.790846710165603 27.097146302155405 27.454362908336556 22.657644079342436 58-59 22.34003920129614 27.22107053052769 27.890074970461363 22.548815297714807 60-61 22.439536031589338 27.77868479003721 27.397059381881693 22.384719796491762 62-63 22.54066949542253 27.26824473974323 27.281342882322207 22.909742882512035 64-65 21.879235397961313 27.44988705605437 27.65721987054156 23.013657675442758 66-67 22.362173863117647 27.0819473929453 27.41471543658427 23.141163307352784 68-69 22.078852400454856 27.30539638021114 27.38870152610663 23.22704969322738 70-71 22.180173939448576 26.94230011909111 27.679668632539865 23.197857308920447 72-73 22.676956858076817 26.97316900313532 27.646590873321337 22.703283265466524 74-75 22.612393357962368 27.335183523727885 27.29508126534756 22.757341852962192 76-77 22.593910964206845 27.039796517911814 27.327383520122044 23.038908997759297 78-79 22.25153391713998 27.261506753034592 27.44253746936915 23.044421860456282 80-81 22.016234305651153 27.349773336814376 27.718557899983388 22.915434457551086 82-83 22.597443031077926 26.701426560469592 28.19803837256873 22.503092035883753 84-85 22.76710630720927 27.014971696716362 27.290933457860188 22.92698853821418 86-87 22.111660965159917 27.351147971892598 27.71482660618799 22.822364456759487 88-89 22.722163466077934 26.69975673532832 27.538775496647578 23.039304301946164 90-91 22.594710890785603 26.68793154745664 27.71863924376491 22.99871831799285 92-93 22.782769886956554 26.778043616024167 27.651198928383046 22.787987568636233 94-95 21.983867044186624 26.836794064635193 28.011177650768122 23.16816124041006 96-97 22.219525560247327 27.043748494978203 27.575364855205052 23.161361089569414 98-99 22.04440310659618 27.395947847943088 27.259182145842015 23.300466899618716 100-101 22.3378253589923 26.940397702600976 27.1484075020052 23.573369436401524 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 89.0 1 58.0 2 61.0 3 80.5 4 60.5 5 61.0 6 91.0 7 158.0 8 234.5 9 254.0 10 253.5 11 279.0 12 328.5 13 395.0 14 467.5 15 556.5 16 671.0 17 810.5 18 935.5 19 1091.5 20 1286.0 21 1482.5 22 1761.5 23 2148.0 24 2571.0 25 3297.0 26 4354.0 27 5491.5 28 7077.5 29 9521.0 30 12592.0 31 15502.5 32 18997.5 33 22617.5 34 25868.5 35 28855.5 36 31477.0 37 34864.5 38 36177.0 39 35067.5 40 34105.5 41 33214.5 42 32917.0 43 33828.0 44 35320.0 45 37709.5 46 40333.5 47 41640.0 48 41826.5 49 40647.5 50 39334.0 51 38514.0 52 36774.5 53 34968.5 54 33029.5 55 29982.5 56 27087.0 57 23215.0 58 19423.0 59 17765.0 60 15423.5 61 12063.5 62 9539.0 63 7911.5 64 6280.0 65 4661.5 66 3528.0 67 2877.0 68 2443.5 69 1992.0 70 1692.0 71 1433.0 72 1167.5 73 967.5 74 822.0 75 687.5 76 586.5 77 496.0 78 408.0 79 335.5 80 261.0 81 214.5 82 176.0 83 144.5 84 118.0 85 91.5 86 78.5 87 59.0 88 50.0 89 40.5 90 31.0 91 24.0 92 24.0 93 19.5 94 11.5 95 11.0 96 10.0 97 6.5 98 3.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.893534148941609E-4 2 0.0037870682978832183 3 9.467670744708045E-5 4 9.467670744708046E-4 5 0.002272240978729931 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.7338353723540225E-5 12-13 1.4201506117062068E-4 14-15 0.0038817450053302985 16-17 0.004781173726077563 18-19 0.0035030381755419763 20-21 0.0 22-23 0.0 24-25 4.7338353723540225E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 2.8403012234124136E-4 42-43 0.0 44-45 7.100753058531034E-4 46-47 0.6782165937971608 48-49 0.48843713371948805 50-51 0.3759612052723565 52-53 0.31853978220570217 54-55 0.3103029086578062 56-57 0.27868088837048133 58-59 0.2787755650779284 60-61 0.25619517035179973 62-63 0.25008852272146304 64-65 0.24672749960709164 66-67 0.23565032483578324 68-69 0.21486878755114908 70-71 0.22826554165491098 72-73 0.7431174767521345 74-75 0.48881584054927635 76-77 0.5783800057942144 78-79 0.3186817972668728 80-81 0.2746571283039804 82-83 0.25685790730392927 84-85 0.24975715424539824 86-87 0.22826554165491098 88-89 0.23058512098736444 90-91 0.23976876160973123 92-93 0.2005252663729164 94-95 0.17046541175846835 96-97 0.13481963140464256 98-99 0.14225175293923836 100-101 0.13898540653231412 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1056226.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.54318841322748 #Duplication Level Percentage of deduplicated Percentage of total 1 92.23551156291421 62.29880535884338 2 4.752608646857761 6.420126825780957 3 1.0384526462935073 2.1042120824045116 4 0.4405210266856525 1.1901677882166974 5 0.249094920052936 0.8412332558956644 6 0.1798167231832651 0.7287236888289867 7 0.12156565817185729 0.5747652508135835 8 0.10716067707426417 0.5790379041692841 9 0.07693288508291804 0.467666311709584 >10 0.6216065819333223 8.717531688161218 >50 0.10071995614678603 4.845351797474706 >100 0.07124064224004795 8.515500012142924 >500 0.003365698844778015 1.4047101512161664 >1k 0.0014023745186575063 1.3121678843423839 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 1707 0.16161313961216633 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1706 0.16151846290471925 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 1560 0.14769566361744552 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 1425 0.13491430811208963 No Hit GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 1354 0.12819226188334693 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 1290 0.12213295260673379 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 1250 0.11834588430885058 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 1237 0.11711508711203852 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1188 0.11247592844713158 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 1136 0.1075527396598834 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 9.467670744708045E-5 0.0 0.0 0.0 3 0.0 9.467670744708045E-5 0.0 0.0 0.0 4 0.0 9.467670744708045E-5 0.0 0.0 0.0 5 0.0 9.467670744708045E-5 0.0 0.0 0.0 6 0.0 9.467670744708045E-5 0.0 0.0 0.0 7 0.0 9.467670744708045E-5 0.0 0.0 0.0 8 0.0 9.467670744708045E-5 0.0 0.0 0.0 9 0.0 9.467670744708045E-5 0.0 0.0 0.0 10-11 0.0 9.467670744708045E-5 0.0 0.0 0.0 12-13 0.0 9.467670744708045E-5 0.0 0.0 0.0 14-15 0.0 9.467670744708045E-5 0.0 0.0 0.0 16-17 0.0 9.467670744708045E-5 0.0 0.0 0.0 18-19 0.0 9.467670744708045E-5 0.0 9.467670744708045E-5 0.0 20-21 0.0 9.467670744708045E-5 0.0 9.467670744708045E-5 0.0 22-23 0.0 9.467670744708045E-5 0.0 2.3669176861770114E-4 0.0 24-25 0.0 9.467670744708045E-5 0.0 2.8403012234124136E-4 0.0 26-27 0.0 9.467670744708045E-5 0.0 5.680602446824827E-4 0.0 28-29 0.0 9.467670744708045E-5 0.0 0.0015148273191532872 0.0 30-31 0.0 9.467670744708045E-5 0.0 0.006816722936189793 0.0 32-33 0.0 9.467670744708045E-5 0.0 0.022107011188893286 0.0 34-35 0.0 9.467670744708045E-5 0.0 0.04383531554799825 0.0 36-37 0.0 9.467670744708045E-5 0.0 0.07853432882735323 0.0 38-39 0.0 9.467670744708045E-5 0.0 0.1283816152982411 0.0 40-41 0.0 9.467670744708045E-5 0.0 0.18163726323722384 0.0 42-43 0.0 9.467670744708045E-5 0.0 0.2381592575831309 0.0 44-45 0.0 9.467670744708045E-5 0.0 0.30632648694502884 0.0 46-47 0.0 9.467670744708045E-5 0.0 0.38486081577238207 0.0 48-49 0.0 9.467670744708045E-5 0.0 0.4682709950332599 0.0 50-51 0.0 9.467670744708045E-5 0.0 0.5538587385654206 4.7338353723540225E-5 52-53 0.0 9.467670744708045E-5 0.0 0.6380736698395987 9.467670744708045E-5 54-55 0.0 9.467670744708045E-5 0.0 0.7371055058292448 9.467670744708045E-5 56-57 0.0 1.893534148941609E-4 0.0 0.851474968425318 9.467670744708045E-5 58-59 0.0 1.893534148941609E-4 0.0 0.9807560124443064 9.467670744708045E-5 60-61 0.0 1.893534148941609E-4 0.0 1.12267639690748 9.467670744708045E-5 62-63 0.0 1.893534148941609E-4 0.0 1.2653068566765067 9.467670744708045E-5 64-65 0.0 1.893534148941609E-4 0.0 1.4253104922620727 9.467670744708045E-5 66-67 0.0 1.893534148941609E-4 0.0 1.5941190616402172 9.467670744708045E-5 68-69 0.0 1.893534148941609E-4 0.0 1.7813895889705424 9.467670744708045E-5 70-71 0.0 1.893534148941609E-4 0.0 1.982719607356759 9.467670744708045E-5 72-73 0.0 1.893534148941609E-4 0.0 2.2004760344850443 9.467670744708045E-5 74-75 0.0 1.893534148941609E-4 0.0 2.4208834094218474 9.467670744708045E-5 76-77 0.0 1.893534148941609E-4 0.0 2.6630664270714792 9.467670744708045E-5 78-79 0.0 1.893534148941609E-4 0.0 2.9289186215828806 9.467670744708045E-5 80-81 0.0 1.893534148941609E-4 0.0 3.2142268794746576 9.467670744708045E-5 82-83 0.0 1.893534148941609E-4 0.0 3.5218788403239456 9.467670744708045E-5 84-85 0.0 1.893534148941609E-4 0.0 3.834264636545588 9.467670744708045E-5 86-87 0.0 1.893534148941609E-4 0.0 4.163218856570468 9.467670744708045E-5 88-89 0.0 1.893534148941609E-4 0.0 4.523700420175228 9.467670744708045E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2180 0.0 63.23842 2 GTACCTG 2315 0.0 61.18778 1 ACCTGGG 2370 0.0 58.368565 3 CCTGGGG 2015 0.0 50.546066 4 GTACCCG 230 0.0 49.434242 1 CTGGGGG 1795 0.0 45.920723 5 TAACGCA 210 0.0 36.093136 4 ATAACGC 200 0.0 35.53086 3 GTACACG 270 0.0 35.092213 1 TGGGGGG 2745 0.0 32.096184 6 GTATAAC 325 0.0 32.068886 1 GGGGAAT 2435 0.0 30.737001 9 GGGGGGA 2720 0.0 29.778996 7 TACACGG 310 0.0 29.035976 2 GTATCAA 3195 0.0 28.320896 1 TACCGGG 120 5.4929933E-6 27.635117 2 GGGGGAA 2900 0.0 26.46186 8 CAACGCA 3445 0.0 25.989416 5 ATCAACG 3450 0.0 25.678345 3 GGTATCA 2170 0.0 25.542925 1 >>END_MODULE