FastQCFastQC Report
Sat 18 Jun 2016
SRR1931061_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931061_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056226
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26120.24729555985177418No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC18760.17761350317072294No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA17800.1685245392558032No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA17520.16587359144728497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17430.16502150108026123No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC15270.14457133227169183No Hit
GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA14800.14012152702167907No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC14600.13822799287273746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13680.12951773578760606No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC12850.12165956906949839No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT12340.11683105698969729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG22900.061.1854442
ACCTGGG24300.057.8558123
GTACCTG25050.056.748841
CCTGGGG19200.052.9388054
CTGGGGG15000.049.7135
GTACCCG1200.047.5437661
GTACACG2100.045.279771
TGGGGGG23650.037.354546
TATCACG801.0780701E-535.622372
TACACGG2700.031.6643312
GGGGGGA23850.031.2660397
ACACGGG3050.029.5879823
CTAGACG650.005845966729.2286134
TAAGTCG650.005845966729.2286135
GGGGAAT26450.027.8334859
CTGTGCG7300.026.6761159
GTATCAA38000.026.1490691
GGGGGAA28200.026.1062328
TAGTACT8800.025.3674474
CTAGTAC8850.025.2241293
Produced by FastQC (version 0.11.5)