##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931061_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1056226 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.659004796322 33.0 33.0 33.0 33.0 33.0 2 32.607609545684355 33.0 33.0 33.0 33.0 33.0 3 32.66877637929761 33.0 33.0 33.0 33.0 33.0 4 36.61421229926171 37.0 37.0 37.0 37.0 37.0 5 36.57088823793393 37.0 37.0 37.0 37.0 37.0 6 36.57328071833111 37.0 37.0 37.0 37.0 37.0 7 36.51930458064846 37.0 37.0 37.0 37.0 37.0 8 36.528248689200986 37.0 37.0 37.0 37.0 37.0 9 36.52285495717773 37.0 37.0 37.0 37.0 37.0 10-11 36.49826836302079 37.0 37.0 37.0 37.0 37.0 12-13 36.41037240136107 37.0 37.0 37.0 37.0 37.0 14-15 38.37765213126737 40.0 37.0 40.0 37.0 40.0 16-17 38.37773497338638 40.0 37.0 40.0 37.0 40.0 18-19 38.341773919596754 40.0 37.0 40.0 37.0 40.0 20-21 38.26105255882737 40.0 37.0 40.0 37.0 40.0 22-23 38.12333724032546 40.0 37.0 40.0 35.0 40.0 24-25 38.27804182059521 40.0 37.0 40.0 37.0 40.0 26-27 38.223822837157954 40.0 37.0 40.0 37.0 40.0 28-29 38.1419639357486 40.0 37.0 40.0 37.0 40.0 30-31 38.004206959495406 40.0 37.0 40.0 35.0 40.0 32-33 37.86914400895263 40.0 37.0 40.0 33.0 40.0 34-35 37.77422824281925 40.0 37.0 40.0 33.0 40.0 36-37 37.69746294827054 40.0 37.0 40.0 33.0 40.0 38-39 37.54929532126647 40.0 37.0 40.0 33.0 40.0 40-41 37.36300517124176 40.0 37.0 40.0 33.0 40.0 42-43 37.171997280884966 40.0 37.0 40.0 33.0 40.0 44-45 36.93880618352512 40.0 37.0 40.0 33.0 40.0 46-47 36.66233599627353 40.0 37.0 40.0 33.0 40.0 48-49 36.3932709476949 38.5 37.0 40.0 33.0 40.0 50-51 36.141971509885195 37.0 37.0 40.0 33.0 40.0 52-53 35.914827887213534 37.0 37.0 40.0 33.0 40.0 54-55 35.67503024920803 37.0 37.0 40.0 30.0 40.0 56-57 35.37615197883787 37.0 37.0 40.0 27.0 40.0 58-59 35.10832577497619 37.0 35.0 40.0 27.0 40.0 60-61 34.85863300089186 37.0 33.0 40.0 27.0 40.0 62-63 34.53776180476527 37.0 33.0 40.0 27.0 40.0 64-65 34.190914160416426 37.0 33.0 40.0 27.0 40.0 66-67 33.85243404347176 37.0 33.0 37.0 27.0 40.0 68-69 33.54589879438681 37.0 33.0 37.0 27.0 40.0 70-71 33.25012402648676 37.0 33.0 37.0 27.0 40.0 72-73 32.901500247106206 37.0 33.0 37.0 22.0 40.0 74-75 32.5223702124356 37.0 33.0 37.0 22.0 38.5 76-77 30.899893109997294 33.0 30.0 37.0 22.0 37.0 78-79 31.662697661295972 33.0 33.0 37.0 22.0 37.0 80-81 31.732103261991277 33.0 33.0 37.0 22.0 37.0 82-83 31.49224503089301 33.0 33.0 37.0 22.0 37.0 84-85 31.205123240670083 33.0 33.0 37.0 18.5 37.0 86-87 30.856311054641715 33.0 33.0 37.0 15.0 37.0 88-89 30.536207213228987 33.0 33.0 37.0 15.0 37.0 90-91 30.128632508572977 33.0 33.0 37.0 10.5 37.0 92-93 29.738402103337734 33.0 27.0 37.0 4.0 37.0 94-95 29.269140789944576 33.0 27.0 37.0 2.0 37.0 96-97 28.79786570298402 33.0 27.0 37.0 2.0 37.0 98-99 28.152621692705917 33.0 27.0 37.0 2.0 37.0 100-101 26.466422432320357 33.0 21.0 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.009111948464926911 1101 2 0.1586540689205691 1101 3 0.17875614681679508 1101 4 0.1792821994970879 1101 5 0.07352328328233426 1101 6 0.041290994920416324 1101 7 0.03399228586452807 1101 8 0.1790561408070701 1101 9 0.19989221708618743 1101 10-11 0.15672393388666706 1101 12-13 0.1930729117907859 1101 14-15 0.25012156703899535 1101 16-17 0.09469484516714033 1101 18-19 0.20230911897376558 1101 20-21 0.11583773016757704 1101 22-23 0.12409766663022737 1101 24-25 0.28170899153445816 1101 26-27 0.17132913818479523 1101 28-29 0.16610239260822368 1101 30-31 0.13492449126417938 1101 32-33 0.11970300067500972 1101 34-35 0.2710627353119648 1101 36-37 0.010897225035414237 1101 38-39 -0.01703171689985794 1101 40-41 -0.07740684155201194 1101 42-43 -0.11525075780047445 1101 44-45 -0.2656724490814284 1101 46-47 -0.36583763826656934 1101 48-49 -0.17543295165457806 1101 50-51 -0.0018347235201403578 1101 52-53 -0.2183629894180612 1101 54-55 -0.22329309646522688 1101 56-57 -0.20401877602380836 1101 58-59 -0.28540000649710606 1101 60-61 -0.6064685346438452 1101 62-63 -0.49622871056495654 1101 64-65 -0.33704755956606647 1101 66-67 -0.279866734920148 1101 68-69 -0.49063561004217604 1101 70-71 -0.5819818073168932 1101 72-73 -0.36334968360346664 1101 74-75 -0.6715297530446236 1101 76-77 -0.8219970872068849 1101 78-79 -0.5488223759763109 1101 80-81 -0.6827197186328497 1101 82-83 -0.8653592634677061 1101 84-85 -0.9606751903842472 1101 86-87 -1.3768592078921031 1101 88-89 -1.0284625342204237 1101 90-91 -1.4364620670304653 1101 92-93 -1.6759240981269343 1101 94-95 -1.5876597687029488 1101 96-97 -1.9074770909466991 1101 98-99 -1.8582912328864474 1101 100-101 -3.041680152321664 1102 1 0.042252566855019325 1102 2 0.23746935839631078 1102 3 0.0914805656942761 1102 4 0.14869353903876714 1102 5 0.14533597560765799 1102 6 0.15937368840542376 1102 7 0.14993361130122906 1102 8 0.10017747330974203 1102 9 0.16463515852759514 1102 10-11 0.11404827563175246 1102 12-13 0.1673441319660327 1102 14-15 0.15047850666265816 1102 16-17 0.20009586622752806 1102 18-19 0.17733480729953754 1102 20-21 0.12557502152578337 1102 22-23 0.15524189612878558 1102 24-25 0.29019827525493724 1102 26-27 0.2835493646735685 1102 28-29 0.2379636298011505 1102 30-31 0.2880700638374094 1102 32-33 0.3659947121782636 1102 34-35 0.19908350712141498 1102 36-37 0.2549551983950735 1102 38-39 0.2838102668591844 1102 40-41 0.42330986947926874 1102 42-43 0.5052409747388751 1102 44-45 0.3537560160107631 1102 46-47 0.3734905816740408 1102 48-49 0.23138180382761675 1102 50-51 -0.018886782496764454 1102 52-53 0.18854548462795861 1102 54-55 0.29203764241462693 1102 56-57 0.0018188791512088187 1102 58-59 0.1698092623362797 1102 60-61 0.04530564541985882 1102 62-63 0.10104846175044457 1102 64-65 0.29200964516650885 1102 66-67 0.07099736372302345 1102 68-69 -0.07441056682376512 1102 70-71 0.10235653962249103 1102 72-73 -0.053158767426957354 1102 74-75 0.02155610934146779 1102 76-77 -0.15101801813563043 1102 78-79 0.18855798115209765 1102 80-81 0.12873201563254355 1102 82-83 0.006825471328983923 1102 84-85 -0.049125287608827506 1102 86-87 0.03415265593415029 1102 88-89 -0.19210256323946595 1102 90-91 -0.15601904117492538 1102 92-93 -0.13825395510835747 1102 94-95 -0.48532853628360684 1102 96-97 -0.624273749981036 1102 98-99 -0.7095969549778722 1102 100-101 -1.8627626489302678 1103 1 0.027371774779687996 1103 2 0.17252319660258308 1103 3 0.09369872917243072 1103 4 0.16999378435883727 1103 5 0.08094313053144475 1103 6 0.15795856865887004 1103 7 0.07732180949633971 1103 8 0.0714074523032977 1103 9 0.15882778129780206 1103 10-11 0.1509931838937959 1103 12-13 0.194099198731287 1103 14-15 0.2860545681248894 1103 16-17 0.14234527006548348 1103 18-19 0.139229402913152 1103 20-21 0.1816289429743918 1103 22-23 0.2246880407843861 1103 24-25 0.34378185790723137 1103 26-27 0.2728233445876853 1103 28-29 0.2092731786766393 1103 30-31 0.38880675852899316 1103 32-33 0.2622221204525559 1103 34-35 0.3188790247663462 1103 36-37 0.2925942008580691 1103 38-39 0.2394849460089361 1103 40-41 0.3374294838224188 1103 42-43 0.2715576971452691 1103 44-45 0.4131016822677509 1103 46-47 0.34261134672009064 1103 48-49 0.46224953175248373 1103 50-51 0.5973354353729192 1103 52-53 0.5493398123971147 1103 54-55 0.46692244970861907 1103 56-57 0.4276854563731618 1103 58-59 0.6392825831669384 1103 60-61 0.5066199929713093 1103 62-63 0.27436635444617963 1103 64-65 0.41632350795376283 1103 66-67 0.5524869608790723 1103 68-69 0.31374723126950244 1103 70-71 0.24587734775869308 1103 72-73 0.1772890763516486 1103 74-75 0.13513048637049252 1103 76-77 0.09601463734391658 1103 78-79 0.0699547875842832 1103 80-81 0.21551460133542477 1103 82-83 0.1000825971179502 1103 84-85 0.13992295867097226 1103 86-87 -0.006240688596093946 1103 88-89 -0.016282503794645464 1103 90-91 -0.031172672254680123 1103 92-93 0.11082915502125346 1103 94-95 -0.20387639817966985 1103 96-97 -0.16621931928517597 1103 98-99 -0.41843851542894583 1103 100-101 -1.6134567431312163 1104 1 0.1213476886230751 1104 2 0.1869222525135399 1104 3 0.060553732547212746 1104 4 0.17497317486105857 1104 5 0.15842720115134057 1104 6 0.1660750176275485 1104 7 0.155561490951186 1104 8 0.15705042193877716 1104 9 0.12179436037587266 1104 10-11 0.12466460355071263 1104 12-13 0.14720038690809645 1104 14-15 0.26208302663633276 1104 16-17 0.27686628496120846 1104 18-19 0.27267855412446806 1104 20-21 0.3130808194787207 1104 22-23 0.3167441512563798 1104 24-25 0.36154401878442144 1104 26-27 0.2829253945004453 1104 28-29 0.2785824315133638 1104 30-31 0.25489914681108417 1104 32-33 0.2877643136766679 1104 34-35 0.3061556844570532 1104 36-37 0.2552169345561879 1104 38-39 0.25983655974747677 1104 40-41 0.22259467817995215 1104 42-43 0.1689718525379149 1104 44-45 0.15608999576983962 1104 46-47 0.15779127124390868 1104 48-49 0.18498492787509235 1104 50-51 0.12427848014498011 1104 52-53 0.07169188459615583 1104 54-55 0.0023321563363438713 1104 56-57 0.16218694424731694 1104 58-59 0.25912755559563294 1104 60-61 0.08355115173024075 1104 62-63 -0.01455609356312948 1104 64-65 0.02999518231096232 1104 66-67 0.08368880660710687 1104 68-69 -0.12899083920606813 1104 70-71 0.031209583050809897 1104 72-73 -0.0633697908263926 1104 74-75 -0.012763354552198791 1104 76-77 0.08136724865855527 1104 78-79 0.18741937363264682 1104 80-81 0.05262422009155543 1104 82-83 -0.12272178033088821 1104 84-85 -0.004084580457359266 1104 86-87 -0.0576880229453991 1104 88-89 -0.28004480900198914 1104 90-91 -0.1738833624499918 1104 92-93 -0.12925663652354658 1104 94-95 0.3531647911008413 1104 96-97 0.45428575137292526 1104 98-99 -0.0557336408900575 1104 100-101 -0.8573004250784848 1105 1 0.0036874064421184016 1105 2 0.17949089024325815 1105 3 0.10999976166076664 1105 4 0.13400040981971273 1105 5 0.05248714828205436 1105 6 0.03675233774791309 1105 7 0.23862820811169172 1105 8 0.07680252952795996 1105 9 0.16482667705432164 1105 10-11 0.0307759126159155 1105 12-13 0.18108504828535388 1105 14-15 0.20070316435019464 1105 16-17 0.03734546651329396 1105 18-19 0.14524846041859973 1105 20-21 0.16717865564361745 1105 22-23 0.17604963089499392 1105 24-25 0.10403850146198579 1105 26-27 0.09903578945305469 1105 28-29 0.12575959586147434 1105 30-31 0.2808095749702275 1105 32-33 0.27773425989327194 1105 34-35 0.1666872875680241 1105 36-37 0.22818671847116434 1105 38-39 0.1712963602470623 1105 40-41 0.12789307997535104 1105 42-43 0.17324952636388247 1105 44-45 0.26381116904267543 1105 46-47 0.290628154381146 1105 48-49 0.2783097605831628 1105 50-51 0.23927104598440252 1105 52-53 0.2570058263056225 1105 54-55 0.19204130063367586 1105 56-57 0.2646958102066961 1105 58-59 0.33596664752737837 1105 60-61 0.2670270741911054 1105 62-63 0.23207941243550323 1105 64-65 0.41647032684335983 1105 66-67 0.4027854581567212 1105 68-69 0.32669055711023987 1105 70-71 0.02838842638124106 1105 72-73 0.21332742709990526 1105 74-75 0.108507672369349 1105 76-77 0.07152328208149683 1105 78-79 0.20244953002052313 1105 80-81 -0.05503539049648154 1105 82-83 -0.1335796656713164 1105 84-85 0.24473406893195815 1105 86-87 0.17412580030253366 1105 88-89 0.38721354346862924 1105 90-91 0.20725448386343004 1105 92-93 0.30162805656781444 1105 94-95 0.10386529983248138 1105 96-97 0.1480403102762864 1105 98-99 -0.13670909230311068 1105 100-101 -0.5977698685331596 1106 1 -0.025719708481730663 1106 2 0.16319694026678633 1106 3 0.06570165015720875 1106 4 0.0877947739609013 1106 5 0.060298058185367154 1106 6 0.021564089233933714 1106 7 -0.046544316342959746 1106 8 0.10109766851217472 1106 9 0.1942091787606799 1106 10-11 0.1149488564419272 1106 12-13 0.10955004497676413 1106 14-15 0.21711308044752542 1106 16-17 0.22365947626202143 1106 18-19 0.28739452471779003 1106 20-21 0.26660138102732844 1106 22-23 0.04166335673972554 1106 24-25 0.09895066540873643 1106 26-27 0.15112587761570495 1106 28-29 0.11457004852695007 1106 30-31 0.22048447223371426 1106 32-33 0.10858607601739578 1106 34-35 0.1704403299037054 1106 36-37 0.06298185603538542 1106 38-39 0.2997674155766319 1106 40-41 0.22642452288735626 1106 42-43 0.13973910408626722 1106 44-45 0.21015624824516976 1106 46-47 0.08885941070603565 1106 48-49 0.20032325430631914 1106 50-51 0.09187905304272448 1106 52-53 -0.01961029175451756 1106 54-55 0.21291094240723396 1106 56-57 0.20150516095734616 1106 58-59 0.09803067407936084 1106 60-61 -0.0031564741449940925 1106 62-63 0.03651935287151531 1106 64-65 0.1438027368654744 1106 66-67 0.17630031081348108 1106 68-69 -0.0717856007286457 1106 70-71 -0.03424369216358514 1106 72-73 0.20262635746627922 1106 74-75 0.33292057613261505 1106 76-77 0.06907900025258229 1106 78-79 0.373248219461356 1106 80-81 0.30868453759505954 1106 82-83 0.27741609990860994 1106 84-85 0.5869941113613599 1106 86-87 0.4497749029140934 1106 88-89 0.29076286409649654 1106 90-91 0.15574611962946605 1106 92-93 0.4584085719875226 1106 94-95 0.5267359961877389 1106 96-97 0.10134051674331701 1106 98-99 0.3225314195382154 1106 100-101 0.32638734176690676 1107 1 0.081637895593623 1107 2 0.1767960655959726 1107 3 0.1328107861153356 1107 4 0.13365141498500321 1107 5 0.14231635571134404 1107 6 0.10153987639737494 1107 7 0.05386552889725493 1107 8 0.058040188993246034 1107 9 0.051870114876933826 1107 10-11 0.07204584120951552 1107 12-13 0.0549556502842492 1107 14-15 0.09729625270519904 1107 16-17 0.13715455086603612 1107 18-19 0.16181282116922802 1107 20-21 0.1333403399648958 1107 22-23 0.04797669269373017 1107 24-25 0.06316957309103088 1107 26-27 0.08994817025971003 1107 28-29 0.0908734360854524 1107 30-31 0.12487421293966605 1107 32-33 0.0030108554916381536 1107 34-35 0.047687785272479744 1107 36-37 0.08611702437459456 1107 38-39 0.02293831217689757 1107 40-41 0.07131551587333718 1107 42-43 0.1930807710290381 1107 44-45 0.26404368900028174 1107 46-47 0.4792551347764089 1107 48-49 0.3595701133086351 1107 50-51 0.34261219875045157 1107 52-53 0.13946419405849753 1107 54-55 0.044075656854253964 1107 56-57 0.2565584712837108 1107 58-59 0.38536961707801254 1107 60-61 0.37500702160119204 1107 62-63 0.19688551829784018 1107 64-65 0.4231381912098655 1107 66-67 0.4558197791339751 1107 68-69 0.309771184372579 1107 70-71 0.05978162923405961 1107 72-73 0.17903137804808722 1107 74-75 0.3639710317798759 1107 76-77 0.11623717078652973 1107 78-79 0.36663311849543945 1107 80-81 0.477470125942812 1107 82-83 0.3915746098162636 1107 84-85 0.5889233479137879 1107 86-87 0.5109432080961227 1107 88-89 0.5619215611521824 1107 90-91 0.6169787177724899 1107 92-93 0.7863272978049061 1107 94-95 0.9215507420201128 1107 96-97 0.8252613486476683 1107 98-99 0.8679860056948137 1107 100-101 1.6826934693875373 1108 1 0.016443949037920902 1108 2 -0.013206569940599877 1108 3 0.06690829471210691 1108 4 0.06426563577727507 1108 5 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-0.10777070315376847 2316 58-59 -0.16306688072069875 2316 60-61 -0.13612800703619143 2316 62-63 -0.07669677725991875 2316 64-65 -0.11601853154175501 2316 66-67 0.0375884931911159 2316 68-69 0.011933036056731794 2316 70-71 0.0028630258062989355 2316 72-73 0.038425289574192334 2316 74-75 -0.08876461971769345 2316 76-77 -0.1676661175442753 2316 78-79 -0.07331254490287265 2316 80-81 0.0809953026030108 2316 82-83 0.20618953461244516 2316 84-85 0.3097043703178919 2316 86-87 0.42706867794952075 2316 88-89 0.3524271518523001 2316 90-91 0.5115686720477548 2316 92-93 0.6585482167730738 2316 94-95 0.6210335623173115 2316 96-97 0.888376210705804 2316 98-99 1.046067359402553 2316 100-101 1.4091107841524853 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 8.0 5 26.0 6 38.0 7 69.0 8 226.0 9 463.0 10 805.0 11 1288.0 12 1978.0 13 2933.0 14 3423.0 15 4189.0 16 5275.0 17 6913.0 18 7014.0 19 5420.0 20 4798.0 21 4640.0 22 5011.0 23 5582.0 24 6411.0 25 7641.0 26 9063.0 27 11286.0 28 13588.0 29 17299.0 30 21811.0 31 28072.0 32 36806.0 33 49966.0 34 71773.0 35 110933.0 36 182425.0 37 295733.0 38 133288.0 39 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.82100222381615 21.068076062661017 13.105923575388504 25.004998138134326 2 13.957171963522889 21.786571787802586 40.86415381585724 23.39210243281728 3 17.03934574608086 28.87365014684357 29.8327251932825 24.25427891379307 4 11.629802712677021 17.848642241338503 38.88760549352127 31.633949552463204 5 12.129695727997607 37.723176668629634 35.10593376796254 15.041193835410224 6 28.442776451251913 38.52243743289788 19.854557642019795 13.180228473830411 7 26.52945487045386 31.762236491053997 24.189709399314165 17.51859923917798 8 26.023313192441773 35.062477159244324 21.077875378943524 17.83633426937038 9 26.797958012773783 15.2235411739533 21.601153541003534 36.37734727226938 10-11 24.894435471196505 26.46744162707602 28.863661754207907 19.774461147519563 12-13 25.156263905641406 24.534995351373663 28.87748455349518 21.43125618948975 14-15 22.02537254294913 25.918546364135143 27.016517857776854 25.03956323513887 16-17 21.294637700643612 27.7507843965212 28.029938668428915 22.92463923440627 18-19 21.187416329459793 27.79959023921017 29.80067712781166 21.212316303518374 20-21 22.19212554888821 27.225754715373412 29.152662403690123 21.429457332048255 22-23 22.078655515012883 27.283412830208686 28.63719506999449 22.00073658478394 24-25 21.711925288716618 27.122557104256096 29.560719012787036 21.60479859424025 26-27 21.743405293942775 27.31834853525666 28.921130515628285 22.017115655172283 28-29 21.392060032606658 27.612982448831975 29.295624231935214 21.699333286626157 30-31 22.124952424954508 27.3120051958577 28.99180667773753 21.57123570145026 32-33 22.210492830132942 27.54268499355252 28.555962455004895 21.690859721309643 34-35 21.96518548113756 27.266465699575658 29.186319973187558 21.582028846099224 36-37 22.162065694273764 27.091171775737386 28.90115373035695 21.845608799631897 38-39 22.08747090465057 27.276467004441763 29.112959306511726 21.523102784395945 40-41 21.866603041509734 26.762331634768877 29.343600464312157 22.027464859409232 42-43 22.04039295573212 27.025672028439157 28.712223032818745 22.221711983009982 44-45 21.888639363166597 27.737813687600948 28.57418772118846 21.799359228044 46-47 21.988002567632307 27.371130799658406 28.370112078286276 22.27075455442301 48-49 22.41299079883112 27.002119341105985 28.611957022394385 21.97293283766851 50-51 21.973517031392902 27.543821114041883 28.04683846070822 22.435823393856996 52-53 21.883290129195835 27.70619166731362 27.979712675128237 22.430805528362303 54-55 21.99552936587435 27.612177696818673 27.86434910710397 22.527943830203007 56-57 22.572725912825476 27.2611164656049 27.77246536252658 22.393692259043046 58-59 21.964759435954047 27.5036308517306 28.302702262583956 22.228907449731402 60-61 22.271417291375144 28.056022101330587 27.52786808883705 22.144692518457223 62-63 22.310708124965682 27.526305923164173 27.50367819008432 22.65930776178583 64-65 21.614550295107296 27.618663051278798 27.90435001600036 22.86243663761354 66-67 22.041441407322544 27.232694874975145 27.693360096194812 23.032503621507495 68-69 21.753007890059926 27.485539308746294 27.747815391724007 23.013637409469776 70-71 21.76559307559321 27.220305508584495 27.942891822239226 23.07120959358307 72-73 22.269192388750135 27.2416130638708 27.935214622628113 22.55397992475095 74-75 22.250372789888516 27.679945087457693 27.484816208667663 22.58486591398613 76-77 22.144259737210355 27.38400463545937 27.611753767730917 22.85998185959936 78-79 21.731154513297128 27.674905886184774 27.775600093924453 22.81833950659365 80-81 21.479631351414813 27.669142250104624 28.008430365221145 22.842796033259418 82-83 22.028320956959 27.150274716691015 28.4452730077004 22.376131318649584 84-85 22.1798113333488 27.477332686992018 27.528695992194674 22.814159987464514 86-87 21.456521450982844 27.86611148035167 27.875389832819238 22.801977235846252 88-89 21.93938607835823 27.218227917131372 27.719635759771112 23.122750244739287 90-91 21.863786727461736 27.116450456625763 27.986860766540495 23.03290204937201 92-93 21.941460782132427 27.261258361223806 27.836472687852375 22.96080816879139 94-95 21.00202949033613 27.418996730186667 28.20688587560886 23.37208790386834 96-97 21.194916598220477 27.703829593843864 27.693225365881368 23.408028442054285 98-99 20.87782349610784 28.088496211984936 27.310443030184828 23.723237261722396 100-101 19.693208435347874 28.445251093046725 27.57545288774519 24.286087583860212 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 85.0 1 56.0 2 62.5 3 90.0 4 71.0 5 68.5 6 108.0 7 190.0 8 299.0 9 372.5 10 388.0 11 435.5 12 488.5 13 529.5 14 639.0 15 753.0 16 829.0 17 937.5 18 1071.5 19 1206.0 20 1335.5 21 1551.0 22 1874.5 23 2317.0 24 2786.0 25 3375.5 26 4393.0 27 5602.5 28 7176.0 29 9539.5 30 12549.0 31 15721.5 32 19562.5 33 23043.5 34 26158.0 35 29371.0 36 32040.0 37 35576.5 38 36910.0 39 35732.0 40 34849.0 41 33760.0 42 33283.0 43 34165.0 44 35973.5 45 39031.0 46 41887.0 47 43112.0 48 43346.5 49 42001.5 50 40665.5 51 39734.0 52 37860.5 53 35682.0 54 33529.5 55 30302.0 56 26588.5 57 22369.0 58 18741.5 59 17222.5 60 14455.0 61 10711.5 62 7996.0 63 6158.5 64 4547.0 65 3005.5 66 2155.5 67 1680.5 68 1298.0 69 973.0 70 774.0 71 641.0 72 526.5 73 421.5 74 324.0 75 277.0 76 216.0 77 156.0 78 127.0 79 93.0 80 69.0 81 52.0 82 43.0 83 45.0 84 37.5 85 26.5 86 18.5 87 11.0 88 7.0 89 7.5 90 8.0 91 4.5 92 2.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07886569730341801 2 1.893534148941609E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 5.207218909589425E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 4.7338353723540225E-5 40-41 0.0037870682978832183 42-43 0.007242768119701655 44-45 0.0 46-47 0.0 48-49 1.4201506117062068E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.003408361468094896 68-69 0.008236873547896 70-71 0.0018935341489416091 72-73 0.0 74-75 9.467670744708045E-5 76-77 0.0018935341489416091 78-79 0.005491249031930667 80-81 0.00416577512767154 82-83 0.0044498052500127816 84-85 0.002319579332453471 86-87 3.787068297883218E-4 88-89 0.0 90-91 0.0 92-93 0.001041443781917885 94-95 0.004686497018630483 96-97 0.004118436773948 98-99 0.0 100-101 3.787068297883218E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1056226.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.10920707130294 #Duplication Level Percentage of deduplicated Percentage of total 1 90.19746752318308 56.020931877045 2 6.106389921652943 7.5852607220412 3 1.2549601215094606 2.3383373415917577 4 0.5520675337208942 1.3715390707685815 5 0.3404478399031447 1.0572472692761106 6 0.2072514684116452 0.7723334620446283 7 0.15507956746887178 0.6742308277916578 8 0.1187134066596248 0.5898556445089944 9 0.09620950667113848 0.5377946554859078 >10 0.750377987792715 9.592192242856418 >50 0.11921754273121019 5.217506672847925 >100 0.09541587666451475 10.677013070492594 >500 0.0047250669655460225 1.8506607124031733 >1k 0.00167663666519375 1.715096430845992 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2612 0.24729555985177418 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 1876 0.17761350317072294 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 1780 0.1685245392558032 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 1752 0.16587359144728497 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1743 0.16502150108026123 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 1527 0.14457133227169183 No Hit GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 1480 0.14012152702167907 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 1460 0.13822799287273746 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1368 0.12951773578760606 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 1285 0.12165956906949839 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 1234 0.11683105698969729 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 9.467670744708045E-5 14-15 0.0 0.0 0.0 0.0 9.467670744708045E-5 16-17 0.0 0.0 0.0 0.0 9.467670744708045E-5 18-19 0.0 0.0 0.0 0.0 9.467670744708045E-5 20-21 0.0 0.0 0.0 0.0 9.467670744708045E-5 22-23 0.0 0.0 0.0 1.4201506117062068E-4 9.467670744708045E-5 24-25 0.0 0.0 0.0 1.893534148941609E-4 9.467670744708045E-5 26-27 0.0 0.0 0.0 4.733835372354023E-4 1.893534148941609E-4 28-29 0.0 0.0 0.0 0.0014201506117062068 1.893534148941609E-4 30-31 0.0 0.0 0.0 0.006816722936189793 1.893534148941609E-4 32-33 0.0 0.0 0.0 0.021822981066552047 1.893534148941609E-4 34-35 0.0 0.0 0.0 0.04317257859586869 1.893534148941609E-4 36-37 0.0 0.0 0.0 0.07754022339915889 1.893534148941609E-4 38-39 0.0 0.0 0.0 0.1268194496253643 1.893534148941609E-4 40-41 0.0 0.0 0.0 0.17955437567338808 1.893534148941609E-4 42-43 0.0 0.0 0.0 0.23574500154323033 2.3669176861770114E-4 44-45 0.0 0.0 0.0 0.3038175541976812 2.8403012234124136E-4 46-47 0.0 0.0 0.0 0.3813577775968401 2.8403012234124136E-4 48-49 0.0 0.0 0.0 0.4639632048444178 2.8403012234124136E-4 50-51 4.7338353723540225E-5 0.0 0.0 0.5488882114244489 2.8403012234124136E-4 52-53 9.467670744708045E-5 0.0 0.0 0.6310202551347912 2.8403012234124136E-4 54-55 9.467670744708045E-5 0.0 0.0 0.7279692035606016 2.8403012234124136E-4 56-57 9.467670744708045E-5 0.0 0.0 0.8406818237763509 3.313684760647816E-4 58-59 9.467670744708045E-5 0.0 0.0 0.9658444310213913 3.787068297883218E-4 60-61 9.467670744708045E-5 0.0 0.0 1.106013296396794 3.787068297883218E-4 62-63 9.467670744708045E-5 0.0 0.0 1.2469869137854968 4.2604518351186207E-4 64-65 9.467670744708045E-5 0.0 0.0 1.4029194509508383 4.733835372354023E-4 66-67 9.467670744708045E-5 0.0 0.0 1.5700711779486587 4.733835372354023E-4 68-69 9.467670744708045E-5 0.0 0.0 1.7554955094837656 4.733835372354023E-4 70-71 9.467670744708045E-5 0.0 0.0 1.9538432115853994 4.733835372354023E-4 72-73 9.467670744708045E-5 0.0 0.0 2.168854014197719 4.733835372354023E-4 74-75 9.467670744708045E-5 0.0 0.0 2.3874151933393044 4.733835372354023E-4 76-77 9.467670744708045E-5 0.0 0.0 2.624959052324029 4.733835372354023E-4 78-79 9.467670744708045E-5 0.0 0.0 2.887876268904572 4.733835372354023E-4 80-81 9.467670744708045E-5 0.0 0.0 3.169113428376124 5.207218909589425E-4 82-83 1.4201506117062068E-4 0.0 0.0 3.4708480950099694 5.680602446824827E-4 84-85 1.893534148941609E-4 0.0 0.0 3.7765118450028687 5.680602446824827E-4 86-87 1.893534148941609E-4 0.0 0.0 4.097276529833577 5.680602446824827E-4 88-89 1.893534148941609E-4 0.0 0.0 4.448101069278733 5.680602446824827E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2290 0.0 61.185444 2 ACCTGGG 2430 0.0 57.855812 3 GTACCTG 2505 0.0 56.74884 1 CCTGGGG 1920 0.0 52.938805 4 CTGGGGG 1500 0.0 49.713 5 GTACCCG 120 0.0 47.543766 1 GTACACG 210 0.0 45.27977 1 TGGGGGG 2365 0.0 37.35454 6 TATCACG 80 1.0780701E-5 35.62237 2 TACACGG 270 0.0 31.664331 2 GGGGGGA 2385 0.0 31.266039 7 ACACGGG 305 0.0 29.587982 3 CTAGACG 65 0.0058459667 29.228613 4 TAAGTCG 65 0.0058459667 29.228613 5 GGGGAAT 2645 0.0 27.833485 9 CTGTGCG 730 0.0 26.676115 9 GTATCAA 3800 0.0 26.149069 1 GGGGGAA 2820 0.0 26.106232 8 TAGTACT 880 0.0 25.367447 4 CTAGTAC 885 0.0 25.224129 3 >>END_MODULE