##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931060_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4274830 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.218529157884642 33.0 33.0 33.0 27.0 33.0 2 31.164313902541153 33.0 33.0 33.0 27.0 33.0 3 31.170742930128217 33.0 33.0 33.0 27.0 33.0 4 34.794962606700146 37.0 37.0 37.0 33.0 37.0 5 34.78962251130454 37.0 37.0 37.0 33.0 37.0 6 34.88647665521202 37.0 37.0 37.0 33.0 37.0 7 34.845090214113775 37.0 37.0 37.0 33.0 37.0 8 34.849018557463104 37.0 37.0 37.0 33.0 37.0 9 34.85898784279141 37.0 37.0 37.0 33.0 37.0 10-11 34.76967306302239 37.0 37.0 37.0 33.0 37.0 12-13 34.697509725532946 37.0 37.0 37.0 33.0 37.0 14-15 36.35951488129352 40.0 37.0 40.0 33.0 40.0 16-17 36.30994612651263 40.0 37.0 40.0 33.0 40.0 18-19 36.23962438272399 40.0 37.0 40.0 33.0 40.0 20-21 36.15854887796708 40.0 37.0 40.0 33.0 40.0 22-23 36.0874564602569 40.0 37.0 40.0 33.0 40.0 24-25 35.96495041908099 40.0 37.0 40.0 33.0 40.0 26-27 35.78700217318583 40.0 37.0 40.0 27.0 40.0 28-29 35.69733790583486 40.0 37.0 40.0 27.0 40.0 30-31 35.58801157005074 40.0 37.0 40.0 27.0 40.0 32-33 35.434333061197755 37.0 37.0 40.0 27.0 40.0 34-35 35.352241843535296 37.0 37.0 40.0 27.0 40.0 36-37 35.17237574359682 37.0 37.0 40.0 27.0 40.0 38-39 35.02088141516739 37.0 37.0 40.0 27.0 40.0 40-41 34.80837132704693 37.0 37.0 40.0 27.0 40.0 42-43 34.667344900265036 37.0 37.0 40.0 27.0 40.0 44-45 34.45137900220593 37.0 35.0 40.0 22.0 40.0 46-47 34.36362440143819 37.0 37.0 40.0 22.0 40.0 48-49 34.40714566427203 37.0 37.0 40.0 22.0 40.0 50-51 33.64307878909804 37.0 33.0 38.5 22.0 40.0 52-53 33.77151067995686 37.0 33.0 38.5 22.0 40.0 54-55 34.31497977697359 37.0 33.0 40.0 22.0 40.0 56-57 34.30205236231616 37.0 33.0 40.0 22.0 40.0 58-59 34.17698657022619 37.0 33.0 40.0 22.0 40.0 60-61 34.21516925819272 37.0 33.0 40.0 22.0 40.0 62-63 34.064747720962 37.0 33.0 40.0 22.0 40.0 64-65 33.85791867746788 37.0 33.0 40.0 22.0 40.0 66-67 33.63756547043976 37.0 33.0 40.0 22.0 40.0 68-69 33.43029582462928 37.0 33.0 40.0 22.0 40.0 70-71 33.17592711289104 37.0 33.0 40.0 15.0 40.0 72-73 32.535446204878326 37.0 33.0 38.5 15.0 40.0 74-75 32.30543156102114 37.0 33.0 37.0 15.0 40.0 76-77 31.902370035767504 37.0 33.0 37.0 6.0 40.0 78-79 31.61686663563229 37.0 33.0 37.0 6.0 37.0 80-81 31.315653137083814 37.0 33.0 37.0 6.0 37.0 82-83 31.02917215421432 37.0 33.0 37.0 2.0 37.0 84-85 30.624168446464537 37.0 33.0 37.0 2.0 37.0 86-87 30.429930079090866 37.0 33.0 37.0 2.0 37.0 88-89 30.194408081724887 33.0 33.0 37.0 2.0 37.0 90-91 29.867692750354983 33.0 33.0 37.0 2.0 37.0 92-93 29.558210034083228 33.0 27.0 37.0 2.0 37.0 94-95 29.27470729830192 33.0 27.0 37.0 2.0 37.0 96-97 28.937081240657523 33.0 27.0 37.0 2.0 37.0 98-99 28.554105777305764 33.0 27.0 37.0 2.0 37.0 100-101 27.787748401690827 33.0 27.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26974494820668937 1101 2 0.2707984689035783 1101 3 0.246591427711234 1101 4 0.3593695316418746 1101 5 0.2978282159358514 1101 6 0.26167102486901683 1101 7 0.26649488036490254 1101 8 0.26764778893939223 1101 9 0.3089007297807811 1101 10-11 0.3131521914521258 1101 12-13 0.37461597658531076 1101 14-15 0.5095609402492798 1101 16-17 0.5149998312417665 1101 18-19 0.444204958445944 1101 20-21 0.477704304187931 1101 22-23 0.49614373117690747 1101 24-25 0.5375310299261216 1101 26-27 0.5103642556432888 1101 28-29 0.5408706715485962 1101 30-31 0.4455674837653305 1101 32-33 0.44476850517173716 1101 34-35 0.5505808108642256 1101 36-37 0.49243531621539915 1101 38-39 0.5970477646777468 1101 40-41 0.5186477501006976 1101 42-43 0.5195182804896419 1101 44-45 0.4755442637300149 1101 46-47 0.6003647235180622 1101 48-49 0.5483150673157269 1101 50-51 0.43930438005673267 1101 52-53 0.5543860773934242 1101 54-55 0.6540916289647924 1101 56-57 0.6785856828883539 1101 58-59 0.6672430188707352 1101 60-61 0.6487789996904993 1101 62-63 0.6387864212591765 1101 64-65 0.7040007429219202 1101 66-67 0.6844844591976411 1101 68-69 0.6123111639587862 1101 70-71 0.5228062470578791 1101 72-73 0.42167203904077866 1101 74-75 0.42927222325412373 1101 76-77 0.31082380092992423 1101 78-79 0.3858567895469669 1101 80-81 0.47518350734333836 1101 82-83 0.4755357784566776 1101 84-85 0.5236213916743075 1101 86-87 0.43287951874126307 1101 88-89 0.4465689680542333 1101 90-91 0.33459803286206125 1101 92-93 0.26289218970876504 1101 94-95 0.21538738223938125 1101 96-97 0.17093381792934537 1101 98-99 0.22521653616185588 1101 100-101 0.3084349912411142 1102 1 0.3972427946615795 1102 2 0.33980762545833443 1102 3 0.33238228391822844 1102 4 0.5068554671312313 1102 5 0.27765135936727603 1102 6 0.39005421764586146 1102 7 0.4417881347171928 1102 8 0.3963819298078306 1102 9 0.46432021909813415 1102 10-11 0.4620791410751295 1102 12-13 0.37502940407199503 1102 14-15 0.5140567979983146 1102 16-17 0.5335429180961029 1102 18-19 0.49062183160479833 1102 20-21 0.5405798452329051 1102 22-23 0.5470564620848037 1102 24-25 0.566364606087177 1102 26-27 0.5484128779061663 1102 28-29 0.5435905192583945 1102 30-31 0.5982059753266213 1102 32-33 0.5849018122725909 1102 34-35 0.5406598805327363 1102 36-37 0.6358747138608578 1102 38-39 0.6478249020047997 1102 40-41 0.5859433760113077 1102 42-43 0.5980562094705135 1102 44-45 0.6009164814029191 1102 46-47 0.40953404002006977 1102 48-49 0.5355666120190676 1102 50-51 0.4187034612108178 1102 52-53 0.5822306180236438 1102 54-55 0.6561308500727279 1102 56-57 0.6814078904581962 1102 58-59 0.6809193596519734 1102 60-61 0.6683935417032387 1102 62-63 0.7735850514981379 1102 64-65 0.8021731541480008 1102 66-67 0.7761204622179179 1102 68-69 0.7342762605758537 1102 70-71 0.7446772257776644 1102 72-73 0.6680575395672008 1102 74-75 0.6580677800385857 1102 76-77 0.5814832111244357 1102 78-79 0.6205200920748517 1102 80-81 0.6558499896736372 1102 82-83 0.5960425794052995 1102 84-85 0.6372621059068457 1102 86-87 0.6009131991232621 1102 88-89 0.7265723711872418 1102 90-91 0.7156226417676308 1102 92-93 0.5738707499708049 1102 94-95 0.3614695253623985 1102 96-97 0.4601017519085637 1102 98-99 0.5651855357470978 1102 100-101 0.5400248392699538 1103 1 0.10533800826020112 1103 2 0.06135247260159815 1103 3 0.12184137300724274 1103 4 0.07188360560922291 1103 5 0.01189599114413653 1103 6 0.03567609352083423 1103 7 0.08348150129337029 1103 8 0.07099213861754805 1103 9 0.0871665714762031 1103 10-11 0.11078520174186934 1103 12-13 0.20004067111310775 1103 14-15 0.16649070140531563 1103 16-17 0.2770731232563861 1103 18-19 0.20904744602996317 1103 20-21 0.16013179071023842 1103 22-23 0.1765854493705561 1103 24-25 0.13982054367712493 1103 26-27 0.16519590630617387 1103 28-29 0.14011349008163165 1103 30-31 0.07463321786049448 1103 32-33 0.11332623683538401 1103 34-35 0.09691896894107543 1103 36-37 0.14021345733739565 1103 38-39 0.1287330104652682 1103 40-41 0.09910804471709866 1103 42-43 0.0536092539670463 1103 44-45 -0.026928241950159304 1103 46-47 -0.08424660564454456 1103 48-49 -0.11552048237236079 1103 50-51 0.09253872142743802 1103 52-53 0.20004816565315053 1103 54-55 0.246238577777234 1103 56-57 0.34565278141300126 1103 58-59 0.32722635540131506 1103 60-61 0.22383203085814785 1103 62-63 0.25949418161711435 1103 64-65 0.28104197070879167 1103 66-67 0.20885049998590688 1103 68-69 0.27220822872209993 1103 70-71 0.27006403249466615 1103 72-73 0.05421451186618498 1103 74-75 0.23126735399193876 1103 76-77 0.14946797434116377 1103 78-79 0.10399360402368174 1103 80-81 0.15983422877497233 1103 82-83 0.13321746652872335 1103 84-85 0.10632417889318546 1103 86-87 0.01887287574773211 1103 88-89 1.3334783779228587E-4 1103 90-91 0.026246784140621315 1103 92-93 -0.12303046357100555 1103 94-95 -0.09782634968975046 1103 96-97 -0.085056437610465 1103 98-99 -0.04782743532354061 1103 100-101 -0.05561915181380073 1104 1 0.1290447544989739 1104 2 0.158723409460098 1104 3 0.09241202315588737 1104 4 0.22275647886340977 1104 5 0.11645215674024456 1104 6 0.08854498632944541 1104 7 0.13882680908780998 1104 8 0.16122422086169053 1104 9 0.17526857246441807 1104 10-11 0.14244612224981523 1104 12-13 0.12873134743001202 1104 14-15 0.16841136300947568 1104 16-17 0.2677427232703735 1104 18-19 0.28144205301386904 1104 20-21 0.2581829733884007 1104 22-23 0.30181932125099564 1104 24-25 0.24812390405283224 1104 26-27 0.2984998877638887 1104 28-29 0.22561412358528088 1104 30-31 0.23497828182274105 1104 32-33 0.2614620246848034 1104 34-35 0.2873074718390001 1104 36-37 0.29727663195897236 1104 38-39 0.37995902634840206 1104 40-41 0.3936399467094347 1104 42-43 0.3436073493155547 1104 44-45 0.2643929881050582 1104 46-47 0.18587244887540777 1104 48-49 0.13118249228840995 1104 50-51 0.267011238778629 1104 52-53 0.30792652191131253 1104 54-55 0.37085928069485163 1104 56-57 0.3918764647881616 1104 58-59 0.3089728012020245 1104 60-61 0.35795280416505193 1104 62-63 0.3860134632230583 1104 64-65 0.3525933783566586 1104 66-67 0.4120058726213074 1104 68-69 0.37144480391949486 1104 70-71 0.36917353891389126 1104 72-73 0.3329734151917805 1104 74-75 0.33802923483068525 1104 76-77 0.23412862893400188 1104 78-79 0.3626676353427776 1104 80-81 0.4607738568434634 1104 82-83 0.39427470452642766 1104 84-85 0.42237422614142517 1104 86-87 0.2229290473246266 1104 88-89 0.17816673536628613 1104 90-91 0.20915874783630528 1104 92-93 0.2299685344166882 1104 94-95 0.21667172863651274 1104 96-97 0.13338298113038505 1104 98-99 0.11003044823140584 1104 100-101 0.21650392731756796 1105 1 0.2282054458692997 1105 2 0.1240381545198872 1105 3 0.14712204401321927 1105 4 0.22703292322484003 1105 5 0.22031249218698434 1105 6 0.21610537054704793 1105 7 0.22450529490794224 1105 8 0.1896277185286479 1105 9 0.19368528323239786 1105 10-11 0.21470570088333574 1105 12-13 0.23739907252832637 1105 14-15 0.2519644246786825 1105 16-17 0.22814613792307625 1105 18-19 0.22493105535556168 1105 20-21 0.35680234268765787 1105 22-23 0.3538988281463631 1105 24-25 0.30713449528722236 1105 26-27 0.2462013443180311 1105 28-29 0.27122422641917865 1105 30-31 0.2997500618211575 1105 32-33 0.41559665304039584 1105 34-35 0.3281697650384032 1105 36-37 0.2541383648122064 1105 38-39 0.3472049768096568 1105 40-41 0.34393118836045034 1105 42-43 0.34598778285746334 1105 44-45 0.3899194367901515 1105 46-47 0.30673911761743256 1105 48-49 0.25667236282077965 1105 50-51 0.14996331188650203 1105 52-53 0.1893116146278473 1105 54-55 0.35528727794154236 1105 56-57 0.3421353654719894 1105 58-59 0.339721970194816 1105 60-61 0.3575734463494342 1105 62-63 0.28872482960297674 1105 64-65 0.30838440036581005 1105 66-67 0.3109689727883733 1105 68-69 0.3097422162962218 1105 70-71 0.33859965104539214 1105 72-73 0.2627591000766216 1105 74-75 0.30216431284677725 1105 76-77 0.2826917041967931 1105 78-79 0.31254103861561333 1105 80-81 0.3748276500757264 1105 82-83 0.33615530582629205 1105 84-85 0.3467795501218198 1105 86-87 0.36336589250651485 1105 88-89 0.4244721689946651 1105 90-91 0.39879649441456166 1105 92-93 0.36535621095167414 1105 94-95 0.24330927113807732 1105 96-97 0.3235856569562898 1105 98-99 0.2403688696463142 1105 100-101 0.17922715781700305 1106 1 0.21392788201683288 1106 2 0.07145940267513495 1106 3 0.16020347322278639 1106 4 0.13042743731281092 1106 5 0.0812615855017711 1106 6 0.16222853498305057 1106 7 0.12034245910645325 1106 8 0.11747406549476835 1106 9 0.13955626082395867 1106 10-11 0.11049186227160845 1106 12-13 0.09123666646227235 1106 14-15 0.22121038035938767 1106 16-17 0.23286186228172312 1106 18-19 0.17325681177473484 1106 20-21 0.31439757714791483 1106 22-23 0.24112488594578707 1106 24-25 0.2209875540422317 1106 26-27 0.24448718057752927 1106 28-29 0.2942289817809751 1106 30-31 0.2620456123631243 1106 32-33 0.2523146052379843 1106 34-35 0.22495203619015314 1106 36-37 0.23776298525969963 1106 38-39 0.21980241649119847 1106 40-41 0.3068062880194944 1106 42-43 0.28693037359666107 1106 44-45 0.234505364085706 1106 46-47 0.20804393008881306 1106 48-49 0.20494646617214585 1106 50-51 0.19211473142703284 1106 52-53 0.25700331338331495 1106 54-55 0.3186504383063635 1106 56-57 0.3386089339158431 1106 58-59 0.36701378882384716 1106 60-61 0.37065326405114263 1106 62-63 0.3357536897297493 1106 64-65 0.4249133537904015 1106 66-67 0.3542475431264549 1106 68-69 0.41613198633123005 1106 70-71 0.4014886888338367 1106 72-73 0.18828623278004386 1106 74-75 0.30769941827332303 1106 76-77 0.19071172647517898 1106 78-79 0.3409164125137032 1106 80-81 0.3376627164868147 1106 82-83 0.3645961861053948 1106 84-85 0.49276107149833237 1106 86-87 0.4294797554322187 1106 88-89 0.37199607302795457 1106 90-91 0.27312093335537924 1106 92-93 0.5115214724965718 1106 94-95 0.5014628789366462 1106 96-97 0.5915625926034096 1106 98-99 0.5223081638624159 1106 100-101 0.5294060351897372 1107 1 -0.08149500634138818 1107 2 -0.006099520893894095 1107 3 0.023461912923213646 1107 4 0.04659414197962519 1107 5 -0.04984869893937116 1107 6 -0.05526204164592485 1107 7 -0.0893633930502844 1107 8 -0.05902776051253511 1107 9 0.013113248385245413 1107 10-11 0.012681097695214305 1107 12-13 -0.08786839662464985 1107 14-15 -0.05325247749203754 1107 16-17 0.004191934268682473 1107 18-19 0.01222400764351761 1107 20-21 0.007647470147482238 1107 22-23 -0.01686835936052944 1107 24-25 3.157113548653001E-4 1107 26-27 0.05114070052494668 1107 28-29 -0.009792954306767854 1107 30-31 0.04261137462617626 1107 32-33 -0.016525696789884137 1107 34-35 -0.03174797727656653 1107 36-37 0.04951468559983141 1107 38-39 0.013701725421768174 1107 40-41 -0.01586820267846889 1107 42-43 -0.044914260448543075 1107 44-45 -0.03393480300830731 1107 46-47 -0.2527099662601131 1107 48-49 -0.14530944151815106 1107 50-51 -0.2077282279448056 1107 52-53 -0.12236556409219901 1107 54-55 -0.021216201160186188 1107 56-57 -0.11255241739623756 1107 58-59 -0.03686564002720161 1107 60-61 -0.08666512522241021 1107 62-63 -0.08460689837927049 1107 64-65 -0.11583064918561092 1107 66-67 -0.06355036806519365 1107 68-69 -0.025500980200639844 1107 70-71 -0.006459289624750397 1107 72-73 -0.06217610240263127 1107 74-75 0.0062927459799695384 1107 76-77 -0.1652344439761677 1107 78-79 -0.0939993004824764 1107 80-81 0.031115516964678847 1107 82-83 -0.06478089140291488 1107 84-85 0.02263188431491514 1107 86-87 0.13155264372377218 1107 88-89 0.060364586502011264 1107 90-91 0.08664338744519284 1107 92-93 0.030523735172288013 1107 94-95 0.07175748011504979 1107 96-97 0.19706688128723115 1107 98-99 0.21995236777256366 1107 100-101 0.2907664939649024 1108 1 -0.12652819790774927 1108 2 -0.10587705896622523 1108 3 -0.15005369054993167 1108 4 -0.04600618620532515 1108 5 -0.07841110078791758 1108 6 -0.07660493264633317 1108 7 -0.054804976892263824 1108 8 -0.030957988602843045 1108 9 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-1.0705084330707493 2316 82-83 -0.9816970255169615 2316 84-85 -1.0095579386897633 2316 86-87 -0.8962943640811964 2316 88-89 -0.9147319692588063 2316 90-91 -0.9359105151482652 2316 92-93 -0.9726194313634622 2316 94-95 -0.8979413094292958 2316 96-97 -0.8073093703111525 2316 98-99 -0.6537933139636927 2316 100-101 -0.8203396850960765 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 112547.0 3 17482.0 4 13198.0 5 11101.0 6 11313.0 7 11826.0 8 11703.0 9 12237.0 10 12603.0 11 12579.0 12 12255.0 13 12670.0 14 13086.0 15 14432.0 16 16558.0 17 19815.0 18 18276.0 19 15769.0 20 16894.0 21 18313.0 22 20743.0 23 24341.0 24 28497.0 25 33346.0 26 37960.0 27 45618.0 28 54429.0 29 66056.0 30 81081.0 31 102117.0 32 131273.0 33 173466.0 34 238696.0 35 343039.0 36 532872.0 37 1006490.0 38 969947.0 39 202.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.21918676638569 25.092360808466154 13.631199133716088 23.057253291432065 2 13.60435158839199 21.986497668006574 44.118761008162274 20.290389735439167 3 16.845056202353266 31.834596115252545 30.26798081136029 21.052366871033897 4 10.561033407489024 19.303123662214986 44.41095352052382 25.724889409772178 5 11.759324067125423 39.363386439047574 36.895495964202766 11.98179352962423 6 24.427755021837125 43.060355616480656 21.107786742396772 11.404102619285446 7 23.18789285187949 32.64419871667411 28.742593272714938 15.42531515873146 8 21.987517632280117 38.85972073743284 22.25948166359832 16.893279966688734 9 25.49736480748942 15.845402039379344 24.60228827813036 34.05494487500088 10-11 22.100681255559433 28.151404939731485 31.28481792540167 18.46309587930741 12-13 22.70590397060559 25.787877005066758 30.465680887867613 21.04053813646004 14-15 20.664630488954018 27.301051588471264 27.535876403264247 24.498441519310468 16-17 19.31140916690187 29.087381511633115 30.05165244184378 21.549556879621235 18-19 18.88889408748298 30.759627666293927 30.60742842705619 19.744049819166904 20-21 19.52788765869052 29.00803072870734 30.04973297183748 21.414348640764665 22-23 19.89167990306047 30.218874200845413 29.06688687035508 20.822559025739036 24-25 19.899006486575253 29.756102524802746 29.865276482404322 20.479614506217676 26-27 20.14108163365561 29.184868170196243 29.26214609703778 21.411904099110373 28-29 19.75641136606602 30.46949235408192 28.945876210282044 20.82822006957002 30-31 21.287583365888235 29.728188021511965 28.443692497713357 20.54053611488644 32-33 20.806850798745213 29.865444941670194 28.70316480421444 20.624539455370154 34-35 21.422407440763727 28.64564204892358 29.48967561283139 20.4422748974813 36-37 21.60823939197582 29.434515524593962 29.27121078499028 19.68603429843994 38-39 22.401592577950467 28.409433825438672 30.163597148892467 19.02537644771839 40-41 20.93402083222705 28.47881980500227 30.56834687035872 20.018812492411957 42-43 21.655340680214184 28.724405414952177 29.272146494714406 20.348107410119233 44-45 20.655536641103694 29.83214665096848 29.320345288426157 20.19197141950167 46-47 21.809336407587026 28.602272788871787 28.506243605615957 21.082147197925234 48-49 21.741629069634257 27.28029931181144 29.715159410398257 21.26291220815605 50-51 21.587879583682206 28.34139872622267 28.17212002169041 21.89860166840471 52-53 20.129729285626258 28.588106877307055 29.388454293108605 21.893709543958074 54-55 21.04194321937455 29.376707360281497 28.23701778312885 21.34433163721511 56-57 21.444832919445357 28.04271600769765 29.170649216538045 21.34180185631895 58-59 20.672742390025682 27.650399494394946 30.692383039235892 20.984475076343475 60-61 20.943192579784235 29.916926293990322 28.051820370499392 21.08806075572605 62-63 21.64805363667775 28.56471641333101 27.405418135782035 22.381811814209208 64-65 20.0006239463575 28.604844216310475 28.245629384777164 23.14890245255486 66-67 21.01868491984429 27.07669864373527 29.08989951879017 22.814716917630268 68-69 19.741696746394798 28.1472040361686 28.488592878388168 23.62250633904843 70-71 19.940272696369515 27.970907446717575 29.289174027534642 22.799645829378267 72-73 20.780203321068026 27.746815737333712 29.34189546183602 22.13108547976224 74-75 20.964137980022112 28.727143022122725 28.196954511959145 22.111764485896018 76-77 20.0116234473916 28.82983409571251 29.493279377810065 21.665263079085822 78-79 20.160559776992613 28.22362390288515 28.887682931710856 22.72813338841138 80-81 20.190577854704976 28.753733608465925 29.625699921423653 21.429988615405446 82-83 21.27631944762153 27.465860528189623 29.55474691451436 21.703073109674484 84-85 22.090928956774725 27.251180859363224 28.62088271004189 22.037007473820164 86-87 20.852795354444094 28.69450450999798 28.67529656226987 21.777403573288055 88-89 21.781895728316897 26.976447196607182 29.1814361528408 22.060220922235114 90-91 21.538922183077656 26.45264248116434 29.824712634578752 22.18372270117925 92-93 20.631001863811083 27.89981819079147 29.283849436325802 22.185330509071647 94-95 19.99494689730471 26.969248143553116 29.703032951456116 23.33277200768606 96-97 20.70324167793315 26.98438654150661 28.913104439646055 23.399267340914186 98-99 20.00144322728112 27.88839917042546 29.449298675885828 22.66085892640759 100-101 20.89520943283551 26.14889997654864 28.88015183174808 24.075738758867764 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 29.0 1 19.5 2 14.0 3 18.5 4 19.5 5 19.5 6 25.0 7 46.0 8 75.0 9 87.5 10 91.5 11 113.0 12 187.5 13 352.5 14 462.0 15 614.5 16 881.5 17 1025.5 18 1112.0 19 1186.0 20 1266.0 21 1402.5 22 1555.5 23 1735.0 24 1864.0 25 2024.5 26 2464.5 27 3289.0 28 5907.5 29 13793.5 30 25980.5 31 41751.0 32 66324.5 33 106405.0 34 169238.5 35 218405.5 36 263832.0 37 334914.5 38 358444.0 39 309466.0 40 253408.0 41 208932.5 42 175614.0 43 156924.5 44 137145.0 45 121484.0 46 112589.5 47 108496.0 48 106792.5 49 99512.5 50 90868.5 51 87793.5 52 83724.0 53 80020.5 54 77223.5 55 69495.0 56 62376.5 57 53222.5 58 43591.0 59 40152.5 60 34853.5 61 27593.0 62 21662.5 63 17856.0 64 14560.5 65 10278.0 66 7606.5 67 6043.0 68 4945.0 69 4091.0 70 3365.0 71 2744.5 72 2276.5 73 1882.5 74 1550.0 75 1312.0 76 1070.5 77 884.0 78 725.5 79 604.0 80 510.5 81 434.5 82 380.0 83 328.0 84 287.5 85 239.0 86 186.5 87 148.0 88 137.5 89 119.0 90 89.5 91 76.0 92 52.0 93 38.5 94 38.0 95 23.0 96 17.0 97 14.0 98 6.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.444621189614557E-4 2 0.0037662316396207566 3 2.1053468792911064E-4 4 9.357097241293805E-4 5 0.002011775906878168 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.8481857758086284E-5 12-13 8.18746008613208E-5 14-15 0.0036024824378981155 16-17 0.004713637735301754 18-19 0.003976766327549867 20-21 0.0 22-23 0.0 24-25 2.3392743103234514E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 2.5732017413557966E-4 42-43 0.0 44-45 6.316040637873319E-4 46-47 0.7450354750949161 48-49 0.5366997050175095 50-51 0.4128702194005376 52-53 0.346329561643387 54-55 0.33721808820467714 56-57 0.3043044986584262 58-59 0.30423432042911647 60-61 0.2804555970646786 62-63 0.27549633552679287 64-65 0.27236170795095943 66-67 0.259600966588145 68-69 0.2384071413366146 70-71 0.25225564525372934 72-73 0.8241263395269519 74-75 0.5433900295450346 76-77 0.6405166989096642 78-79 0.36070440228032463 80-81 0.31274927891869386 82-83 0.29322803479904463 84-85 0.284233525075851 86-87 0.25661839184248264 88-89 0.25995185773469354 90-91 0.27073591230528465 92-93 0.2256113108591453 94-95 0.1905221962042935 96-97 0.14664910651417717 98-99 0.15481317385720603 100-101 0.1502048034658688 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4274830.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.780609208411875 #Duplication Level Percentage of deduplicated Percentage of total 1 78.79905260645802 21.102866338466416 2 10.541334570166317 5.64606723317493 3 3.817701888873108 3.0672114708037967 4 1.8003174070745092 1.9285438771985515 5 1.0208961263424112 1.3670110100978796 6 0.6508143561268659 1.0457522963194676 7 0.44099228143226493 0.8267029367074433 8 0.3274713938300135 0.7015906740076425 9 0.26718028809138905 0.6439725795209753 >10 1.7351247965540144 9.202676659711173 >50 0.25537109200444486 4.841619559549532 >100 0.2726309203272053 15.67952087066182 >500 0.039868638047009956 7.311384837018697 >1k 0.026755112508367145 13.856872986129437 >5k 0.002816327632459699 5.067245081803143 >10k+ 0.0016721945317729465 7.710961588829196 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 28465 0.6658744324335705 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 27121 0.6344345857028233 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 22993 0.5378693421726712 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 22628 0.5293309909399906 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 21042 0.49223010037826065 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 20252 0.47374983332670545 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 18745 0.43849696947013095 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 18584 0.43473073783051025 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 16508 0.38616740314819537 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 16353 0.382541527967194 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 16065 0.3758044179534625 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 14092 0.32965053581078074 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 13281 0.3106790211540576 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 13262 0.31023455903509617 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 12502 0.2924560742766379 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 12490 0.2921753613593991 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 11063 0.25879391695108345 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 10912 0.25526161274249504 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 10798 0.2525948400287263 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 9955 0.2328747575926996 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 9709 0.22712014278930392 No Hit TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG 9479 0.22173981187555997 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 9442 0.22087428038074028 No Hit TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG 8903 0.2082655918480969 No Hit CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC 8106 0.189621575594819 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 8046 0.18821801100862492 No Hit ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT 8028 0.18779694163276667 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 7398 0.17305951347772894 No Hit CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT 7354 0.17203023278118662 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 7293 0.17060327545188933 No Hit GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG 7102 0.16613526151917155 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 7021 0.16424044932780954 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 6741 0.15769048125890386 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 6736 0.1575735175433877 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 6435 0.1505323018693141 No Hit GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA 6203 0.14510518546936368 No Hit GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT 6051 0.14154948851767205 No Hit CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC 5849 0.1368241544108187 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 5651 0.13219239127637825 No Hit AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA 5621 0.1314906089832812 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 5561 0.13008704439708713 No Hit ATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTC 5424 0.126882238591944 No Hit GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA 5383 0.1259231361247114 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 5334 0.12477689171265291 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 5326 0.12458974976782702 No Hit AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA 5242 0.12262475934715533 No Hit TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT 5172 0.1209872673299289 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 5144 0.12033227052303834 No Hit GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC 5133 0.12007495034890277 No Hit TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA 5108 0.1194901317713219 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 5040 0.11789942524030195 No Hit ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT 4858 0.11364194599551326 No Hit AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA 4857 0.11361855325241003 No Hit CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC 4843 0.11329105484896476 No Hit CATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCC 4843 0.11329105484896476 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 4661 0.10903357560417606 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 4606 0.10774697473349819 No Hit ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA 4569 0.1068814432386785 No Hit AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA 4503 0.10533752219386502 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 4409 0.10313860434216096 No Hit CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC 4398 0.1028812841680254 No Hit GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG 4279 0.10009754773874048 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 2.3392743103234514E-5 0.0 9 0.0 0.0 0.0 2.3392743103234514E-5 0.0 10-11 0.0 0.0 0.0 2.3392743103234514E-5 0.0 12-13 0.0 2.3392743103234514E-5 0.0 3.508911465485177E-5 0.0 14-15 0.0 2.3392743103234514E-5 0.0 4.678548620646903E-5 0.0 16-17 0.0 2.3392743103234514E-5 0.0 4.678548620646903E-5 0.0 18-19 0.0 2.3392743103234514E-5 0.0 4.678548620646903E-5 0.0 20-21 0.0 2.3392743103234514E-5 0.0 1.1696371551617257E-4 0.0 22-23 0.0 2.3392743103234514E-5 0.0 3.8598026120336944E-4 0.0 24-25 0.0 2.3392743103234514E-5 0.0 9.591024672326151E-4 2.3392743103234514E-5 26-27 0.0 2.3392743103234514E-5 0.0 0.0026667727137687346 2.3392743103234514E-5 28-29 0.0 2.3392743103234514E-5 0.0 0.006491486211147578 2.3392743103234514E-5 30-31 0.0 2.3392743103234514E-5 0.0 0.026328532362690445 2.3392743103234514E-5 32-33 0.0 2.3392743103234514E-5 0.0 0.0902024174060723 2.3392743103234514E-5 34-35 0.0 2.3392743103234514E-5 0.0 0.1931070943172009 2.3392743103234514E-5 36-37 0.0 2.3392743103234514E-5 0.0 0.3534643482898735 2.3392743103234514E-5 38-39 0.0 2.3392743103234514E-5 0.0 0.5821284121239909 2.3392743103234514E-5 40-41 0.0 2.3392743103234514E-5 0.0 0.8164067343028846 2.3392743103234514E-5 42-43 0.0 2.3392743103234514E-5 0.0 1.0790019720082435 2.3392743103234514E-5 44-45 0.0 2.3392743103234514E-5 0.0 1.368019313048706 2.3392743103234514E-5 46-47 0.0 2.3392743103234514E-5 0.0 1.7166881490024166 2.3392743103234514E-5 48-49 0.0 2.3392743103234514E-5 0.0 2.0604211161613444 2.3392743103234514E-5 50-51 0.0 2.3392743103234514E-5 0.0 2.4082829024779935 4.678548620646903E-5 52-53 0.0 2.3392743103234514E-5 0.0 2.762016267313554 4.678548620646903E-5 54-55 0.0 2.3392743103234514E-5 0.0 3.111866436793978 4.678548620646903E-5 56-57 0.0 2.3392743103234514E-5 0.0 3.5285028878341365 4.678548620646903E-5 58-59 0.0 2.3392743103234514E-5 0.0 4.016931667458121 4.678548620646903E-5 60-61 0.0 2.3392743103234514E-5 0.0 4.4985414624675135 4.678548620646903E-5 62-63 0.0 2.3392743103234514E-5 0.0 4.962478040062412 4.678548620646903E-5 64-65 0.0 2.3392743103234514E-5 0.0 5.45425198195016 4.678548620646903E-5 66-67 0.0 2.3392743103234514E-5 0.0 5.963745926738607 5.8481857758086284E-5 68-69 0.0 2.3392743103234514E-5 0.0 6.507135956283642 7.017822930970354E-5 70-71 0.0 2.3392743103234514E-5 0.0 7.055263016307081 7.017822930970354E-5 72-73 0.0 2.3392743103234514E-5 0.0 7.6568892798076185 7.017822930970354E-5 74-75 0.0 2.3392743103234514E-5 0.0 8.207367310512932 7.017822930970354E-5 76-77 0.0 2.3392743103234514E-5 0.0 8.817567014360804 7.017822930970354E-5 78-79 0.0 2.3392743103234514E-5 0.0 9.488587850277087 7.017822930970354E-5 80-81 0.0 2.3392743103234514E-5 0.0 10.22448845919019 7.017822930970354E-5 82-83 0.0 2.3392743103234514E-5 0.0 11.008507940666647 7.017822930970354E-5 84-85 0.0 4.678548620646903E-5 0.0 11.735975465691034 9.357097241293805E-5 86-87 0.0 4.678548620646903E-5 0.0 12.532170869952722 9.357097241293805E-5 88-89 0.0 4.678548620646903E-5 0.0 13.350390541846108 1.1696371551617257E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCTGGG 2860 0.0 48.526295 3 TACCTGG 2835 0.0 48.11995 2 CCTGGGG 2855 0.0 39.652206 4 CTGGGGG 2855 0.0 35.338165 5 GTACCTG 3925 0.0 35.239433 1 GTACACG 430 0.0 31.945911 1 GTACCCG 355 0.0 30.689177 1 TATTCGC 1360 0.0 29.953691 9 TATATCC 4310 0.0 29.782944 4 GAACCGT 830 0.0 29.104324 6 TATTCTC 3525 0.0 29.024525 5 ATATATC 4915 0.0 27.17698 3 TGGGGGG 8685 0.0 26.887009 6 TGAACCG 870 0.0 26.677635 5 GTATCAA 9050 0.0 25.43744 1 GCATCGT 2015 0.0 25.387161 8 CCCATTT 5060 0.0 25.369364 9 ACCGTGT 980 0.0 25.132906 8 GTACTAG 2445 0.0 24.991745 1 TAGGCAT 2735 0.0 24.592434 5 >>END_MODULE