FastQCFastQC Report
Sat 18 Jun 2016
SRR1931059_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931059_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2639787
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA87850.3327920017789314No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA86650.3282461804683484No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC80570.30521401916139446No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC66130.25051263605737883No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC62180.2355493075767098No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC50230.19028050369215394No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC43030.16300557582865588No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG42040.1592552732474249No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40830.15467157009258703No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA39900.15114855857688517No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA39190.14845894763479023No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT38860.1472088467743799No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT38480.14576933669269526No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38290.14504958165185297No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC38170.14459499952079466No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC37970.1438373626356975No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT34220.1296316710401256No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC32910.12466914944273913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30770.11656243477219944No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC30700.11629726186241543No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC29480.11167567686332269No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC29180.11053922153567693No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT26660.1009929967834526No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG35150.055.119652
ACCTGGG36850.052.702373
GTACCTG38650.050.2507861
CCTGGGG35750.045.5791474
CTGGGGG33700.041.1833345
TAACGCA3700.030.7251014
TGGGGGG74250.030.6848626
ATAACGC3900.030.3640173
GTACACG3950.028.7821081
TATCACG2201.8189894E-1227.9916922
GTACTAG17350.026.2107961
GGGGGGA75300.025.8536837
GGGGAAT75150.025.5280749
AGGGCTA35950.025.4297565
TAGGCAT22300.024.6397865
CTATTGA37350.024.6038559
CATGGGG65350.024.5718544
GCTATTG37950.024.2139438
CTGTGCG22250.024.0569449
ATTTAGG38700.023.991291
Produced by FastQC (version 0.11.5)