##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1931059_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2639787
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.713179889134995	33.0	33.0	33.0	27.0	33.0
2	30.616154636718797	33.0	33.0	33.0	27.0	33.0
3	30.646439655926784	33.0	33.0	33.0	27.0	33.0
4	34.1955578234153	37.0	37.0	37.0	27.0	37.0
5	34.14489767545639	37.0	37.0	37.0	27.0	37.0
6	34.24415568377297	37.0	37.0	37.0	27.0	37.0
7	34.20775161026249	37.0	37.0	37.0	27.0	37.0
8	34.20341451791376	37.0	37.0	37.0	27.0	37.0
9	34.23236079274578	37.0	37.0	37.0	27.0	37.0
10-11	34.11283315661453	37.0	37.0	37.0	27.0	37.0
12-13	34.00420564234918	37.0	37.0	37.0	27.0	37.0
14-15	35.51479589072906	40.0	37.0	40.0	27.0	40.0
16-17	35.44315923974169	40.0	37.0	40.0	27.0	40.0
18-19	35.368338809153926	40.0	37.0	40.0	27.0	40.0
20-21	35.26056344697508	40.0	37.0	40.0	27.0	40.0
22-23	35.14726851067908	40.0	37.0	40.0	27.0	40.0
24-25	34.997850015929316	37.0	37.0	40.0	27.0	40.0
26-27	34.800094666728796	37.0	37.0	40.0	24.5	40.0
28-29	34.65629404948203	37.0	37.0	40.0	22.0	40.0
30-31	34.49086782380549	37.0	37.0	40.0	22.0	40.0
32-33	34.3008265439598	37.0	35.0	40.0	22.0	40.0
34-35	34.14035734701322	37.0	33.0	40.0	22.0	40.0
36-37	33.88985399200769	37.0	33.0	40.0	15.0	40.0
38-39	33.663545392109285	37.0	33.0	40.0	15.0	40.0
40-41	33.40736752624359	37.0	33.0	40.0	15.0	40.0
42-43	33.17368371008722	37.0	33.0	40.0	15.0	40.0
44-45	32.84863892427684	37.0	33.0	40.0	6.0	40.0
46-47	32.652131213616855	37.0	33.0	40.0	6.0	40.0
48-49	32.58804782355546	37.0	33.0	40.0	4.0	40.0
50-51	31.771068650614616	37.0	33.0	38.5	2.0	40.0
52-53	31.89877706042192	37.0	33.0	38.5	2.0	40.0
54-55	32.341145327255575	37.0	33.0	40.0	2.0	40.0
56-57	32.24519648744388	37.0	33.0	40.0	2.0	40.0
58-59	32.04236099351955	37.0	33.0	40.0	2.0	40.0
60-61	32.02947396892249	37.0	33.0	40.0	2.0	40.0
62-63	31.83075566324101	37.0	33.0	40.0	2.0	40.0
64-65	31.578490234249962	37.0	33.0	40.0	2.0	40.0
66-67	31.303284696833494	37.0	33.0	37.0	2.0	40.0
68-69	31.029452944498928	37.0	33.0	37.0	2.0	40.0
70-71	30.775401575960487	37.0	33.0	37.0	2.0	40.0
72-73	30.183323881813195	37.0	27.0	37.0	2.0	40.0
74-75	29.996767163411292	37.0	27.0	37.0	2.0	40.0
76-77	29.660776418703477	37.0	27.0	37.0	2.0	37.0
78-79	29.402787611273183	35.0	27.0	37.0	2.0	37.0
80-81	29.157251513095567	33.0	27.0	37.0	2.0	37.0
82-83	28.892290173411716	33.0	27.0	37.0	2.0	37.0
84-85	28.502093161304302	33.0	27.0	37.0	2.0	37.0
86-87	28.32743058436154	33.0	27.0	37.0	2.0	37.0
88-89	28.125648584525948	33.0	27.0	37.0	2.0	37.0
90-91	27.8221479611802	33.0	27.0	37.0	2.0	37.0
92-93	27.517406328616666	33.0	27.0	37.0	2.0	37.0
94-95	27.231207479997437	33.0	27.0	37.0	2.0	37.0
96-97	26.90694628013548	33.0	22.0	37.0	2.0	37.0
98-99	26.549084073828684	33.0	22.0	37.0	2.0	37.0
100-101	25.834129988518015	33.0	18.5	37.0	2.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2768761681665346
1101	2	0.2807701130254401
1101	3	0.232304413756097
1101	4	0.19404875370407382
1101	5	0.17667167496878733
1101	6	0.18529574501577883
1101	7	0.24152735648906543
1101	8	0.23193474700129002
1101	9	0.20985705688224954
1101	10-11	0.2330927569007315
1101	12-13	0.2676672969463283
1101	14-15	0.3885239277815984
1101	16-17	0.37908202953700965
1101	18-19	0.3527503399593357
1101	20-21	0.395741975608054
1101	22-23	0.38214581182878504
1101	24-25	0.24179548714099752
1101	26-27	0.3945957710901453
1101	28-29	0.3076936545806177
1101	30-31	0.2806992670480071
1101	32-33	0.2530622569408294
1101	34-35	0.3158688292893075
1101	36-37	0.35193856897392095
1101	38-39	0.3499472820588707
1101	40-41	0.28100632849506724
1101	42-43	0.3728937719472185
1101	44-45	0.2818813906133997
1101	46-47	0.16004940683947666
1101	48-49	0.272580827216899
1101	50-51	0.31519147621181887
1101	52-53	0.5256421185191726
1101	54-55	0.43428020103450393
1101	56-57	0.47484529039551404
1101	58-59	0.52405724506783
1101	60-61	0.4928537013199161
1101	62-63	0.38905272666968216
1101	64-65	0.5041214046024614
1101	66-67	0.4786948177474173
1101	68-69	0.33713786164645754
1101	70-71	0.22272922952264196
1101	72-73	0.07659506593523702
1101	74-75	0.07386303977508746
1101	76-77	0.026658156552084478
1101	78-79	0.2019547857975077
1101	80-81	0.17440673315154953
1101	82-83	0.09382166708578765
1101	84-85	0.32959629929607104
1101	86-87	0.1550852052354621
1101	88-89	0.05643338363513095
1101	90-91	-0.07264187877370176
1101	92-93	-0.05415139331475771
1101	94-95	-0.2988529685849244
1101	96-97	-0.24246233071407275
1101	98-99	0.03085366887678731
1101	100-101	-0.10693245882679747
1102	1	0.06048892607648071
1102	2	-0.027652643255414944
1102	3	0.04131860101660223
1102	4	0.050235050921166646
1102	5	-0.012883370610353495
1102	6	0.09804409290468641
1102	7	0.022601565140249136
1102	8	0.0033402986611932306
1102	9	0.09559223746348522
1102	10-11	0.10129537336305816
1102	12-13	0.07871643175717224
1102	14-15	0.2311372381659922
1102	16-17	0.19670773518146234
1102	18-19	0.13918018632893592
1102	20-21	0.0758702488420866
1102	22-23	0.11377107832687017
1102	24-25	0.2738097877295971
1102	26-27	0.08166662947960646
1102	28-29	0.0986054499100888
1102	30-31	0.17629682482763087
1102	32-33	0.2032573937490838
1102	34-35	0.1534133636879247
1102	36-37	0.12544812749017353
1102	38-39	0.1628966102357765
1102	40-41	0.06480519238095184
1102	42-43	0.05592767121271436
1102	44-45	0.1708515569932061
1102	46-47	0.2880000099574147
1102	48-49	0.2941739014538811
1102	50-51	0.290820270046769
1102	52-53	0.416682293327753
1102	54-55	0.45709314593580785
1102	56-57	0.356757064475957
1102	58-59	0.542363804639443
1102	60-61	0.4372441378455534
1102	62-63	0.42885921895275203
1102	64-65	0.5116143393042343
1102	66-67	0.5354295166842604
1102	68-69	0.45328658351806794
1102	70-71	0.5486588740265788
1102	72-73	0.39839726591682023
1102	74-75	0.4966855467341418
1102	76-77	0.5261274623466896
1102	78-79	0.3502405213322355
1102	80-81	0.49965215930058804
1102	82-83	0.35132150771815773
1102	84-85	0.4347233330040936
1102	86-87	0.40450404461413925
1102	88-89	0.4784388658815786
1102	90-91	0.5171054704113978
1102	92-93	0.3848883635964846
1102	94-95	0.35806482605040557
1102	96-97	0.49215373495955816
1102	98-99	0.463481888988273
1102	100-101	0.3034074900338979
1103	1	-0.10702325357272713
1103	2	-0.10741997024161876
1103	3	-0.1551884953120748
1103	4	-0.07511282695359256
1103	5	-0.2984551326574305
1103	6	-0.2556940557886307
1103	7	-0.02533627858687737
1103	8	-0.21021136799721063
1103	9	-0.09166542923126997
1103	10-11	-0.1875373462382086
1103	12-13	-0.049991938953517945
1103	14-15	0.027497468327908337
1103	16-17	-0.025769066076733793
1103	18-19	-0.08619932807105357
1103	20-21	-0.09302757757168933
1103	22-23	-0.16343393792092087
1103	24-25	-0.16188711618622875
1103	26-27	0.04701703497229914
1103	28-29	0.15073583534704227
1103	30-31	0.04840241317613447
1103	32-33	-0.048566290857060324
1103	34-35	-0.058988402010150764
1103	36-37	-0.11674335030726013
1103	38-39	-0.03796063221565049
1103	40-41	0.040474061214098356
1103	42-43	-0.10707378423265368
1103	44-45	-0.028479367668907685
1103	46-47	-0.2271970038189295
1103	48-49	-0.2127373163788704
1103	50-51	-0.055530213091707026
1103	52-53	0.0333093936874036
1103	54-55	0.153462544907903
1103	56-57	0.05586570744249286
1103	58-59	0.03977225283070851
1103	60-61	0.07411258441781854
1103	62-63	0.07468088476861823
1103	64-65	-0.03376135888826681
1103	66-67	0.06443748446291409
1103	68-69	0.0625183363467734
1103	70-71	0.0361424569002331
1103	72-73	0.010262119306720052
1103	74-75	-0.0399349289536417
1103	76-77	-0.006683992223099011
1103	78-79	-0.03104122877060078
1103	80-81	-0.05185177337556013
1103	82-83	0.030304364521168026
1103	84-85	-0.05387248436642267
1103	86-87	0.005371364496578934
1103	88-89	0.09391755211498065
1103	90-91	0.016688661256463178
1103	92-93	-0.10318177532338169
1103	94-95	0.008369296630089451
1103	96-97	0.04246357466232453
1103	98-99	-0.0239498198962238
1103	100-101	0.04339382768070266
1104	1	0.04601570812067379
1104	2	0.08520566728116918
1104	3	0.0464860212752356
1104	4	0.046896095177565655
1104	5	-0.05730655383950989
1104	6	-0.010063517192975269
1104	7	-0.024179723100409944
1104	8	0.02788235570739772
1104	9	-2.273351388453193E-4
1104	10-11	-0.0180872954391873
1104	12-13	-0.05382763801740964
1104	14-15	0.0589979346834113
1104	16-17	-0.07522840557723498
1104	18-19	0.05182423106253253
1104	20-21	0.08077899098134367
1104	22-23	0.01941474980802127
1104	24-25	0.02162342609049972
1104	26-27	0.039402404545398895
1104	28-29	-0.03225166054901507
1104	30-31	0.018143384999227408
1104	32-33	0.11011602556625633
1104	34-35	0.03677410036540607
1104	36-37	0.15801723825165936
1104	38-39	0.12003163785173143
1104	40-41	0.27210464006182633
1104	42-43	0.2747910938903999
1104	44-45	0.17636246399664657
1104	46-47	0.13125784235289473
1104	48-49	0.2656061514101751
1104	50-51	0.185279833577642
1104	52-53	0.16086687847419512
1104	54-55	0.17732602711395629
1104	56-57	0.2591446989531434
1104	58-59	0.24677763293387045
1104	60-61	0.21960843223306625
1104	62-63	0.17578815452181829
1104	64-65	0.26186593035184913
1104	66-67	0.24085490635508933
1104	68-69	0.16082155821722566
1104	70-71	0.30716052531430194
1104	72-73	0.2818515519415854
1104	74-75	0.2097874163036728
1104	76-77	0.18755111332258068
1104	78-79	0.21579026548894475
1104	80-81	0.29552323129106384
1104	82-83	0.31765862554781776
1104	84-85	0.3234426046421035
1104	86-87	0.3437621699419182
1104	88-89	0.29473133515912053
1104	90-91	0.5413630996441512
1104	92-93	0.412883714477406
1104	94-95	0.2915609257985814
1104	96-97	0.1855963036923427
1104	98-99	0.1787221643446344
1104	100-101	0.2949566294271513
1105	1	0.035468287403357834
1105	2	-0.09700390096364941
1105	3	-0.10599712064900046
1105	4	-0.07316295025461272
1105	5	-0.15485961737383747
1105	6	-0.08397587032094833
1105	7	-0.06441983653836303
1105	8	-0.09134774466750883
1105	9	-0.09542180934252542
1105	10-11	-0.03103823515258597
1105	12-13	-0.06632215630742166
1105	14-15	-0.10372851664656935
1105	16-17	-0.07365215949876358
1105	18-19	0.05279748904198556
1105	20-21	0.05923485289226704
1105	22-23	-0.07228437090824258
1105	24-25	-0.017169704934296703
1105	26-27	0.0641859860533387
1105	28-29	0.016305457415057845
1105	30-31	-0.022929381283923078
1105	32-33	-0.09814118906202651
1105	34-35	-0.05341834982228022
1105	36-37	-0.18297464009334874
1105	38-39	-0.004085108903147727
1105	40-41	-0.004362349865786541
1105	42-43	0.029980324845745088
1105	44-45	-0.13869758181125036
1105	46-47	-0.30776562227393356
1105	48-49	-0.35248325891485877
1105	50-51	-0.2691779162516532
1105	52-53	-0.27126206355868376
1105	54-55	-0.09199969056837887
1105	56-57	-0.10584143828211978
1105	58-59	-0.09581586439129097
1105	60-61	-0.23526546226451828
1105	62-63	-0.1264790051207001
1105	64-65	-0.1585749736155755
1105	66-67	-0.02845765231520403
1105	68-69	-0.20381546533930006
1105	70-71	-0.1302709169599474
1105	72-73	-0.16732361791871853
1105	74-75	-0.0735487960179313
1105	76-77	-0.13444751232403362
1105	78-79	-0.22056279211291852
1105	80-81	0.018874268948231077
1105	82-83	0.03901429168753978
1105	84-85	0.049965618518243105
1105	86-87	0.07486081222267771
1105	88-89	0.13808369307959367
1105	90-91	-0.007143563323452895
1105	92-93	-0.06326306797693348
1105	94-95	0.060768648582836704
1105	96-97	-0.14723720528835926
1105	98-99	0.001875293368676978
1105	100-101	-0.026941522436864318
1106	1	0.11410404029532728
1106	2	0.08750863382280372
1106	3	0.16464148047489502
1106	4	0.09326616935945964
1106	5	0.12790000678084823
1106	6	0.18512897773835846
1106	7	0.17591285374615495
1106	8	0.11835566364671024
1106	9	0.11093037768437597
1106	10-11	0.10385009829349201
1106	12-13	0.06235564357920964
1106	14-15	0.04601316551313772
1106	16-17	0.03920305304558269
1106	18-19	-0.010298225313711384
1106	20-21	0.12374520660021204
1106	22-23	0.15150798808561206
1106	24-25	0.11437950812791797
1106	26-27	0.17082760336386826
1106	28-29	0.2908465270066216
1106	30-31	0.22659401807271706
1106	32-33	0.22868715539441808
1106	34-35	0.19254228112757943
1106	36-37	0.3099931312643065
1106	38-39	0.10394986183307253
1106	40-41	0.24000518985432961
1106	42-43	0.1680211623332326
1106	44-45	0.06384209165680232
1106	46-47	0.07358866418681487
1106	48-49	0.11353157241895673
1106	50-51	0.15011663898328464
1106	52-53	0.15261756773407598
1106	54-55	0.19721044409863708
1106	56-57	0.2849455753198811
1106	58-59	0.2772022117341848
1106	60-61	0.141682928985972
1106	62-63	0.16205406212883133
1106	64-65	0.1723095062030353
1106	66-67	0.28555568958692845
1106	68-69	0.2926544241138451
1106	70-71	0.32072713056657065
1106	72-73	0.32198370304039514
1106	74-75	0.3779557399255431
1106	76-77	0.3398010361507282
1106	78-79	0.20661849050120296
1106	80-81	0.31868800251141494
1106	82-83	0.4901904995035018
1106	84-85	0.57133289814184
1106	86-87	0.45231204703969397
1106	88-89	0.4474775515357621
1106	90-91	0.3948325579491794
1106	92-93	0.5286596227041436
1106	94-95	0.5486017004365173
1106	96-97	0.5508086591768588
1106	98-99	0.6173804579759512
1106	100-101	0.5968843983398351
1107	1	0.042363768267073
1107	2	0.11917632900113517
1107	3	0.18207041610163088
1107	4	0.15821743828400514
1107	5	0.17874841187180124
1107	6	0.11137450261112747
1107	7	0.04226251301552253
1107	8	0.02713507777232138
1107	9	-0.056088626512561746
1107	10-11	0.0452024906226427
1107	12-13	0.06229251471729924
1107	14-15	0.1366987738674581
1107	16-17	-0.0019677732416880644
1107	18-19	-0.009990566770774478
1107	20-21	0.0278919811353191
1107	22-23	0.005529551307688507
1107	24-25	0.11705687406961829
1107	26-27	0.01051994757464314
1107	28-29	-0.0020058538946159388
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2314	98-99	-0.8228302382081623
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2316	3	-0.7205901276935727
2316	4	-0.570076196220171
2316	5	-0.5316489899066781
2316	6	-0.5824555373801346
2316	7	-0.905964158362714
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2316	9	-0.5635288075980895
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2316	12-13	-0.6433619603405987
2316	14-15	-0.853383863678836
2316	16-17	-0.856946673165055
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2316	20-21	-0.9000099696798642
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2316	26-27	-0.7160590761771957
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2316	32-33	-0.7394674851184959
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2316	40-41	-0.8514045562439279
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2316	54-55	-0.8845157234845402
2316	56-57	-0.8511080409630694
2316	58-59	-0.8908895017374405
2316	60-61	-0.8635526997636838
2316	62-63	-0.7903779821941477
2316	64-65	-0.7651783113195982
2316	66-67	-0.8462379143724377
2316	68-69	-0.9214599977865099
2316	70-71	-0.9682572333873765
2316	72-73	-0.6515454828297358
2316	74-75	-0.781963167686623
2316	76-77	-0.7399958634051451
2316	78-79	-0.7011629923659406
2316	80-81	-0.8170720706154526
2316	82-83	-0.7796798120246287
2316	84-85	-0.7134929862551047
2316	86-87	-0.7115570379493441
2316	88-89	-0.5729398441400448
2316	90-91	-0.5554654072643572
2316	92-93	-0.5683412622891986
2316	94-95	-0.39124566294081475
2316	96-97	-0.28167245834687904
2316	98-99	-0.25735429130793364
2316	100-101	-0.2842480789374804
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	100458.0
3	14651.0
4	11240.0
5	9662.0
6	9886.0
7	10500.0
8	10813.0
9	11060.0
10	11217.0
11	11439.0
12	12546.0
13	13325.0
14	13555.0
15	15004.0
16	18451.0
17	22582.0
18	19781.0
19	15250.0
20	14925.0
21	15442.0
22	16944.0
23	18979.0
24	22023.0
25	24967.0
26	28810.0
27	33342.0
28	39389.0
29	47677.0
30	57894.0
31	72117.0
32	91593.0
33	119645.0
34	162986.0
35	233769.0
36	353410.0
37	543684.0
38	410450.0
39	321.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.38393135169905	21.620146270097365	12.559219084664067	23.436703293539523
2	15.247553108790623	20.73940135077184	41.67742556283422	22.33561997760331
3	18.330869746062366	29.007016488482762	29.118086266214405	23.544027499240467
4	12.117389459647846	17.26361487407946	40.7059732702973	29.913022395975393
5	12.714245233985874	38.03208052938707	35.06590820583302	14.187766030794036
6	27.722918553656033	39.02814886201046	19.711097902974746	13.537834681358763
7	25.8657611390616	31.994702602899398	25.030428591397712	17.109107666641286
8	25.3199595270376	34.809134221814105	21.095565664957057	18.775340586191234
9	26.59953246227821	15.22770587172374	22.5937168415482	35.57904482444985
10-11	24.888499463308666	26.212451731400144	28.712844282234297	20.18620452305689
12-13	25.259523828915707	24.51692589533282	28.55433573889404	21.66921453685743
14-15	22.569068768733274	25.62136131239753	26.659552189501184	25.150017729368017
16-17	21.261620062663148	27.708999175843797	27.868659315115018	23.16072144637804
18-19	21.040193961435012	28.159563586771224	29.53792097586847	21.262321475925294
20-21	22.125591951168786	27.06072497515898	28.66337321912715	22.150309854545082
22-23	22.361823131942085	27.323208274000894	28.08285289684357	22.23211569721345
24-25	22.122811825880614	27.358879212390853	28.895026169730016	21.62328279199852
26-27	22.096725985846586	27.19764890121817	28.452655460459496	22.25296965247575
28-29	21.50262502239764	27.957426110515737	28.475971735598364	22.06397713148826
30-31	22.530851921007265	27.267503022024126	28.048740296092074	22.15290476087654
32-33	22.641353260698686	27.692139555198963	27.630411089985667	22.036096094116683
34-35	22.633814773691967	27.083302554334875	28.624506446921664	21.65837622505149
36-37	22.667965256287722	26.98579468722287	28.453204747201195	21.893035309288212
38-39	22.827372056912168	26.966001423599707	28.706785812643215	21.499840706844907
40-41	22.235121019508817	26.617208018392436	28.876618931646224	22.271052030452527
42-43	22.494872690437788	26.863105569921196	27.97340390471046	22.66861783493056
44-45	22.254209460831404	27.738384585312044	27.959104739507413	22.048301214349138
46-47	22.693171826379388	27.093380380812153	27.62506827334214	22.58837951946632
48-49	23.097052044967096	26.638634422922596	27.82425173043732	22.440061801672986
50-51	22.577773788703865	27.01223794208917	27.340277998307222	23.069710270899744
52-53	21.968584978590687	27.66609225502053	27.304361145042865	23.060961621345918
54-55	22.211682528462397	27.63346188086029	27.148534624699177	23.006320965978137
56-57	23.012506679416685	26.888515430426075	27.228313242369794	22.870664647787446
58-59	22.624135098042007	26.814795819027132	27.95209262346075	22.608976459470114
60-61	22.500949104632067	27.983174971973163	27.10540274606974	22.41047317732503
62-63	22.612685614385445	27.263107780113383	26.782840972816597	23.341365632684578
64-65	21.72291649773709	27.59562711818703	27.151969777445924	23.529486606629966
66-67	22.566325472733475	26.50034063696454	27.186684246622683	23.746649643679305
68-69	21.90973684874949	27.10231407032743	27.090449379438695	23.897499701484374
70-71	22.314978342419504	26.628628408928236	27.491324247035013	23.565069001617246
72-73	23.031884223991447	26.486434885541364	27.79002233804914	22.691658552418044
74-75	22.778863934780823	27.427687838731202	27.103465530530364	22.689982695957617
76-77	22.561356071229415	27.054092901338212	27.279586397055322	23.104964630377054
78-79	22.174260858921684	27.137644255504846	27.427072319573593	23.26102256599988
80-81	22.08115091491071	27.14241124409581	27.95770849722646	22.81872934376702
82-83	22.83949081340202	26.240747729871032	28.547886724425233	22.371874732301713
84-85	23.198074319653212	26.84289234939384	27.13414124593372	22.824892085019226
86-87	22.10906925641261	27.493702314028152	27.57151317439639	22.825715255162848
88-89	22.82273164601812	26.511367285088138	27.383981651477185	23.281919417416553
90-91	22.678535279396904	26.20345553011293	28.015119226081573	23.102889964408593
92-93	23.004412514487875	26.71509309255271	27.477744762060958	22.802749630898457
94-95	21.70636513993076	26.73512094745016	28.05139080649091	23.507123106128173
96-97	22.060547088017195	26.754715155929475	27.5576217803359	23.627115975717434
98-99	21.71966696492949	27.454128575241427	27.237345818765597	23.58885864106349
100-101	22.163489544299797	26.60865352569629	27.106841184094794	24.12101574590912
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	153.0
1	98.5
2	94.5
3	141.5
4	107.0
5	85.5
6	135.5
7	246.5
8	391.5
9	480.0
10	440.0
11	437.5
12	539.5
13	683.5
14	835.5
15	1031.0
16	1303.5
17	1643.5
18	1959.5
19	2285.0
20	2637.0
21	2932.5
22	3280.5
23	3712.5
24	4354.0
25	5170.0
26	6500.5
27	8454.5
28	11814.5
29	19628.0
30	29974.0
31	39532.5
32	52073.5
33	64227.5
34	76916.5
35	87982.5
36	94243.0
37	106794.0
38	107468.5
39	92041.0
40	80168.5
41	71235.5
42	67290.5
43	68796.0
44	72295.5
45	80885.0
46	90168.5
47	97946.0
48	106956.0
49	106316.0
50	99747.0
51	97662.0
52	93929.0
53	90004.5
54	86184.0
55	78156.0
56	70729.0
57	60352.0
58	50082.5
59	46054.0
60	39804.0
61	31232.5
62	24546.5
63	20276.0
64	16085.5
65	11599.0
66	8879.0
67	7033.5
68	5716.5
69	4723.0
70	3897.0
71	3191.0
72	2611.5
73	2195.0
74	1866.5
75	1535.0
76	1282.5
77	1062.5
78	850.5
79	686.0
80	587.0
81	477.0
82	381.5
83	310.5
84	241.5
85	193.5
86	167.0
87	147.5
88	113.0
89	88.0
90	68.0
91	53.5
92	50.5
93	41.0
94	30.5
95	23.5
96	11.5
97	7.5
98	7.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.924639753131597E-4
2	0.004204884712289287
3	1.894092212742922E-4
4	0.0010228097948811778
5	0.0020835014340172143
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.682276638228766E-5
12-13	1.894092212742922E-5
14-15	0.003750302581230986
16-17	0.004451116699945867
18-19	0.003295720450172684
20-21	0.0
22-23	0.0
24-25	1.894092212742922E-5
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	2.2729106552915064E-4
42-43	3.788184425485844E-5
44-45	7.197550408423104E-4
46-47	0.7155312152078936
48-49	0.5168788239354161
50-51	0.3933082479760677
52-53	0.329155344730465
54-55	0.3206508706952493
56-57	0.28917105811946187
58-59	0.2888301215211682
60-61	0.2667828881648406
62-63	0.26045662017427923
64-65	0.2570851360355968
66-67	0.245663759992757
68-69	0.22431734075514426
70-71	0.23857985511709845
72-73	0.7931321731639712
74-75	0.5231861510038499
76-77	0.6139889316827457
78-79	0.34402396860049694
80-81	0.2978838822980793
82-83	0.280041533654041
84-85	0.2711961230205316
86-87	0.2454175280051004
88-89	0.2481450207914502
90-91	0.2594148694572706
92-93	0.21547193012163482
94-95	0.18338600803776972
96-97	0.14165915659104314
98-99	0.15033409892540572
100-101	0.14518216810674497
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2639787.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.044416861559014
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.33890965362811	36.73363601641691
2	8.420315947753213	7.248951795222454
3	2.3016386509108266	2.9721808066344573
4	1.038453340453294	1.7879847391100026
5	0.5695648671589713	1.225829378584462
6	0.3465699438353062	0.8950740680480409
7	0.24949306932904708	0.7517498576188543
8	0.18396843705543803	0.6335051275187004
9	0.15293668231705476	0.5924763276371213
>10	1.0261192641392018	8.97474057227243
>50	0.15917872031430383	4.8824616016012286
>100	0.1774840635626058	16.171732090817198
>500	0.024049804489016866	7.039046115234939
>1k	0.010787044660514918	8.440347217786087
>5k	5.30510393140078E-4	1.6502842854971729
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	8785	0.3327920017789314	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	8665	0.3282461804683484	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	8057	0.30521401916139446	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	6613	0.25051263605737883	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	6218	0.2355493075767098	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	5023	0.19028050369215394	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	4303	0.16300557582865588	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	4204	0.1592552732474249	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4083	0.15467157009258703	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	3990	0.15114855857688517	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	3919	0.14845894763479023	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	3886	0.1472088467743799	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	3848	0.14576933669269526	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	3829	0.14504958165185297	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	3817	0.14459499952079466	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	3797	0.1438373626356975	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	3422	0.1296316710401256	No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC	3291	0.12466914944273913	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3077	0.11656243477219944	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	3070	0.11629726186241543	No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC	2948	0.11167567686332269	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	2918	0.11053922153567693	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	2666	0.1009929967834526	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.788184425485844E-5	0.0
10-11	0.0	0.0	0.0	3.788184425485844E-5	0.0
12-13	0.0	0.0	0.0	3.788184425485844E-5	3.788184425485844E-5
14-15	0.0	0.0	0.0	3.788184425485844E-5	7.576368850971688E-5
16-17	0.0	0.0	0.0	3.788184425485844E-5	7.576368850971688E-5
18-19	0.0	0.0	0.0	3.788184425485844E-5	9.47046106371461E-5
20-21	0.0	0.0	0.0	5.682276638228766E-5	1.1364553276457532E-4
22-23	0.0	0.0	0.0	9.47046106371461E-5	1.1364553276457532E-4
24-25	0.0	0.0	0.0	3.0305475403886756E-4	1.5152737701943375E-4
26-27	0.0	0.0	0.0	6.81873196587452E-4	1.70468299146863E-4
28-29	0.0	0.0	0.0	0.0020266786676349266	2.0835014340172143E-4
30-31	0.0	0.0	0.0	0.007803659916500838	2.2729106552915064E-4
32-33	0.0	0.0	0.0	0.02360038897077681	2.2729106552915064E-4
34-35	0.0	0.0	0.0	0.049019106465786826	2.2729106552915064E-4
36-37	0.0	0.0	0.0	0.08959056166274021	2.2729106552915064E-4
38-39	0.0	0.0	0.0	0.14436770845526553	2.2729106552915064E-4
40-41	0.0	0.0	0.0	0.20255422123072808	2.2729106552915064E-4
42-43	0.0	1.894092212742922E-5	0.0	0.2653054962389011	2.2729106552915064E-4
44-45	0.0	3.788184425485844E-5	0.0	0.3392508562243848	2.2729106552915064E-4
46-47	0.0	3.788184425485844E-5	0.0	0.42745873057182265	2.2729106552915064E-4
48-49	0.0	5.682276638228766E-5	0.0	0.5232619146923596	2.651729097840091E-4
50-51	0.0	7.576368850971688E-5	0.0	0.621243304857551	2.651729097840091E-4
52-53	0.0	7.576368850971688E-5	0.0	0.7205126777274076	3.40936598293726E-4
54-55	0.0	7.576368850971688E-5	0.0	0.830767785431173	3.40936598293726E-4
56-57	0.0	7.576368850971688E-5	0.0	0.9626344852823352	3.40936598293726E-4
58-59	0.0	7.576368850971688E-5	0.0	1.1159044271374925	3.40936598293726E-4
60-61	0.0	7.576368850971688E-5	0.0	1.2741747724342911	3.40936598293726E-4
62-63	0.0	7.576368850971688E-5	0.0	1.4346043828536166	3.40936598293726E-4
64-65	0.0	7.576368850971688E-5	0.0	1.6030649442549723	3.40936598293726E-4
66-67	0.0	7.576368850971688E-5	0.0	1.787606348542515	3.40936598293726E-4
68-69	0.0	7.576368850971688E-5	0.0	2.0020933507135235	3.40936598293726E-4
70-71	0.0	7.576368850971688E-5	0.0	2.2429461164859132	3.788184425485844E-4
72-73	0.0	7.576368850971688E-5	0.0	2.4918866560067157	3.788184425485844E-4
74-75	0.0	7.576368850971688E-5	0.0	2.741395423191341	3.788184425485844E-4
76-77	0.0	7.576368850971688E-5	0.0	3.015754680207153	3.788184425485844E-4
78-79	0.0	7.576368850971688E-5	0.0	3.3151538362754267	3.788184425485844E-4
80-81	0.0	7.576368850971688E-5	0.0	3.654650924487468	4.1670028680344286E-4
82-83	0.0	7.576368850971688E-5	0.0	4.014566326752878	4.545821310583013E-4
84-85	0.0	7.576368850971688E-5	0.0	4.365162795331593	4.545821310583013E-4
86-87	0.0	7.576368850971688E-5	0.0	4.731650697575221	4.545821310583013E-4
88-89	0.0	7.576368850971688E-5	0.0	5.139998037720467	4.545821310583013E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	3515	0.0	55.11965	2
ACCTGGG	3685	0.0	52.70237	3
GTACCTG	3865	0.0	50.250786	1
CCTGGGG	3575	0.0	45.579147	4
CTGGGGG	3370	0.0	41.183334	5
TAACGCA	370	0.0	30.725101	4
TGGGGGG	7425	0.0	30.684862	6
ATAACGC	390	0.0	30.364017	3
GTACACG	395	0.0	28.782108	1
TATCACG	220	1.8189894E-12	27.991692	2
GTACTAG	1735	0.0	26.210796	1
GGGGGGA	7530	0.0	25.853683	7
GGGGAAT	7515	0.0	25.528074	9
AGGGCTA	3595	0.0	25.429756	5
TAGGCAT	2230	0.0	24.639786	5
CTATTGA	3735	0.0	24.603855	9
CATGGGG	6535	0.0	24.571854	4
GCTATTG	3795	0.0	24.213943	8
CTGTGCG	2225	0.0	24.056944	9
ATTTAGG	3870	0.0	23.99129	1
>>END_MODULE