FastQCFastQC Report
Sat 18 Jun 2016
SRR1931059_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931059_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2639787
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA98840.3744241486150208No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA94380.35752884607735397No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC89830.3402926069413934No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC76350.28922788088584417No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC66040.25017169945908513No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59070.2237680540134488No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC55300.20948659872936717No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC49570.1877803019713333No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA44480.16849844324561036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42910.16255099369759757No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT42690.16171759312399067No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC40870.15482309746960646No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG40370.1529290052568635No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC40350.1528532415683538No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40340.15281535972409896No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT36730.13914001394809505No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC35840.13576852980941267No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA35320.13379867390816003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32690.12383574886913225No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC31080.11773677194410004No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC30250.11459257887094679No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC30120.11410011489563363No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT28730.10883453854420831No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA26680.10106876047196232No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG33950.055.6829952
ACCTGGG36050.050.993
GTACCTG38650.049.2013051
CCTGGGG32750.047.4259224
CTGGGGG29350.046.1228455
GTACACG4300.035.379171
GTACCCG2950.033.842581
TGGGGGG64900.032.6414576
TATCACG2000.030.8739852
GTACTAG19250.028.6479381
AGGGCTA37150.027.6168585
ATTTAGG39550.026.8055361
CTATTGA38950.026.4625439
CTGTGCG24200.026.3007879
GCTATTG38750.026.2313968
GGGGGGA69100.026.1894447
TAGTACT24950.025.5101834
TAGGCAT24750.024.7567625
GGGGAAT74250.024.4369079
TTTAGGG43300.024.3525472
Produced by FastQC (version 0.11.5)