##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931059_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2639787 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.628568895899555 33.0 33.0 33.0 33.0 33.0 2 32.5885796846488 33.0 33.0 33.0 33.0 33.0 3 32.638319682610756 33.0 33.0 33.0 33.0 33.0 4 36.58691667168601 37.0 37.0 37.0 37.0 37.0 5 36.53297633483307 37.0 37.0 37.0 37.0 37.0 6 36.53540493986826 37.0 37.0 37.0 37.0 37.0 7 36.47007239599255 37.0 37.0 37.0 37.0 37.0 8 36.48154642779891 37.0 37.0 37.0 37.0 37.0 9 36.472000581865125 37.0 37.0 37.0 37.0 37.0 10-11 36.44877295024182 37.0 37.0 37.0 37.0 37.0 12-13 36.362424127401184 37.0 37.0 37.0 37.0 37.0 14-15 38.289493053795624 40.0 37.0 40.0 37.0 40.0 16-17 38.287005353083416 40.0 37.0 40.0 37.0 40.0 18-19 38.245505603293 40.0 37.0 40.0 37.0 40.0 20-21 38.160558787508236 40.0 37.0 40.0 35.0 40.0 22-23 38.02510448759692 40.0 37.0 40.0 33.0 40.0 24-25 38.17805205495746 40.0 37.0 40.0 37.0 40.0 26-27 38.10835741671582 40.0 37.0 40.0 35.0 40.0 28-29 38.021390930404614 40.0 37.0 40.0 33.0 40.0 30-31 37.88836883430368 40.0 37.0 40.0 33.0 40.0 32-33 37.75291661789379 40.0 37.0 40.0 33.0 40.0 34-35 37.65683841158396 40.0 37.0 40.0 33.0 40.0 36-37 37.592001362231116 40.0 37.0 40.0 33.0 40.0 38-39 37.42810442660715 40.0 37.0 40.0 33.0 40.0 40-41 37.23511764396142 40.0 37.0 40.0 33.0 40.0 42-43 37.04746954962654 40.0 37.0 40.0 33.0 40.0 44-45 36.79046737482986 40.0 37.0 40.0 33.0 40.0 46-47 36.50459904530176 38.5 37.0 40.0 33.0 40.0 48-49 36.21606231866435 37.0 37.0 40.0 33.0 40.0 50-51 35.95208173992826 37.0 37.0 40.0 33.0 40.0 52-53 35.71623941628624 37.0 37.0 40.0 30.0 40.0 54-55 35.470491179780794 37.0 37.0 40.0 27.0 40.0 56-57 35.14334584570649 37.0 35.0 40.0 27.0 40.0 58-59 34.85692254716005 37.0 33.0 40.0 27.0 40.0 60-61 34.59244969385787 37.0 33.0 40.0 27.0 40.0 62-63 34.26660787404438 37.0 33.0 40.0 27.0 40.0 64-65 33.90030672929293 37.0 33.0 37.0 27.0 40.0 66-67 33.53995265527105 37.0 33.0 37.0 27.0 40.0 68-69 33.23093889772167 37.0 33.0 37.0 24.5 40.0 70-71 32.952241601311016 37.0 33.0 37.0 22.0 40.0 72-73 32.60367541017514 37.0 33.0 37.0 22.0 40.0 74-75 32.20471045580571 37.0 33.0 37.0 22.0 38.5 76-77 30.569819648327687 33.0 30.0 37.0 22.0 37.0 78-79 31.365844668528183 33.0 33.0 37.0 22.0 37.0 80-81 31.423044359260803 33.0 33.0 37.0 22.0 37.0 82-83 31.188211965586618 33.0 33.0 37.0 15.0 37.0 84-85 30.872046115841922 33.0 33.0 37.0 15.0 37.0 86-87 30.53587524296468 33.0 33.0 37.0 15.0 37.0 88-89 30.19005984194937 33.0 33.0 37.0 15.0 37.0 90-91 29.76861409651612 33.0 27.0 37.0 6.0 37.0 92-93 29.353331727143136 33.0 27.0 37.0 2.0 37.0 94-95 28.854048641045658 33.0 27.0 37.0 2.0 37.0 96-97 28.34513182313573 33.0 27.0 37.0 2.0 37.0 98-99 27.664430501400304 33.0 27.0 37.0 2.0 37.0 100-101 25.94717698814336 33.0 21.0 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09048272349347286 1101 2 0.1505605416991571 1101 3 0.12875167774889462 1101 4 0.09731489253540104 1101 5 0.04573643394508764 1101 6 0.03141585975622263 1101 7 0.005016033328146818 1101 8 0.0016481551960367824 1101 9 0.03425180980561038 1101 10-11 0.0419979554230423 1101 12-13 0.09323980912583352 1101 14-15 0.16226699712829884 1101 16-17 0.09159306928267341 1101 18-19 0.12427064919751274 1101 20-21 0.07701394205800938 1101 22-23 0.066340083095767 1101 24-25 0.08216285877829677 1101 26-27 0.1160336648640623 1101 28-29 0.0017422394060702118 1101 30-31 -0.03357088804943942 1101 32-33 -0.012674683104187068 1101 34-35 0.04613017300449229 1101 36-37 -0.03985206560185617 1101 38-39 -0.05975896290477323 1101 40-41 -0.17178694373941994 1101 42-43 -0.19425072154574963 1101 44-45 -0.2636989629006834 1101 46-47 -0.24137536799108972 1101 48-49 -0.3098495844986928 1101 50-51 -0.32362460457403586 1101 52-53 -0.46072317551162456 1101 54-55 -0.4924971854693041 1101 56-57 -0.5154399758164061 1101 58-59 -0.4930235162774963 1101 60-61 -0.6218470914505048 1101 62-63 -0.6317966624449554 1101 64-65 -0.6629001304308204 1101 66-67 -0.7024113035679562 1101 68-69 -0.7709166135706624 1101 70-71 -0.9117193161564998 1101 72-73 -0.928337154811171 1101 74-75 -1.0735878816609805 1101 76-77 -1.2622455482841488 1101 78-79 -1.1943199834991027 1101 80-81 -1.1548407492162873 1101 82-83 -1.3110736159935108 1101 84-85 -1.4957752666199262 1101 86-87 -1.5950330912169335 1101 88-89 -1.6914725333284935 1101 90-91 -1.9414269905977797 1101 92-93 -1.9289409044650014 1101 94-95 -2.1818040506906016 1101 96-97 -2.533202595947646 1101 98-99 -2.7071299658390586 1101 100-101 -3.8551603024202805 1102 1 0.11085592706614023 1102 2 0.21859245772304092 1102 3 0.09932355963268691 1102 4 0.1401210377511859 1102 5 0.1057294925994583 1102 6 0.15714275322073235 1102 7 0.19244794793542752 1102 8 0.17776107247360784 1102 9 0.12629865680560215 1102 10-11 0.13073597628958566 1102 12-13 0.1393078555620164 1102 14-15 0.2597739561814407 1102 16-17 0.20223174391064447 1102 18-19 0.14134565114533615 1102 20-21 0.1561367826266391 1102 22-23 0.12027079713357836 1102 24-25 0.12182361821817267 1102 26-27 0.23393560773254052 1102 28-29 0.21509087203180854 1102 30-31 0.30994692216850694 1102 32-33 0.2061413216555863 1102 34-35 0.19828517750219987 1102 36-37 0.12896482453363944 1102 38-39 0.18287081546797168 1102 40-41 0.08184114887795602 1102 42-43 0.10544276138066522 1102 44-45 -0.03608639623188736 1102 46-47 -0.0854010297209058 1102 48-49 -0.11898276625571214 1102 50-51 -0.010728398940202055 1102 52-53 0.018298555149996787 1102 54-55 -0.045907121509763726 1102 56-57 -0.05640276878723682 1102 58-59 -0.20527870989145214 1102 60-61 -0.20625704165697556 1102 62-63 -0.3183540029179639 1102 64-65 -0.18195447833389977 1102 66-67 -0.3330515010421564 1102 68-69 -0.37751779784922235 1102 70-71 -0.45141531354995124 1102 72-73 -0.4944063699432988 1102 74-75 -0.565360793413813 1102 76-77 -0.6530266621576324 1102 78-79 -0.4340604267536037 1102 80-81 -0.6638250107775221 1102 82-83 -0.8596922527982258 1102 84-85 -0.6327030482933544 1102 86-87 -0.816594291928066 1102 88-89 -1.0676506142589162 1102 90-91 -0.9030073508394523 1102 92-93 -1.070752772749426 1102 94-95 -1.1623452631595264 1102 96-97 -1.3196924707909332 1102 98-99 -1.3667145121371256 1102 100-101 -2.160303804116662 1103 1 0.04829556573241689 1103 2 0.19811676993837324 1103 3 0.11390114180918687 1103 4 0.15048427131794995 1103 5 0.16312046223004728 1103 6 0.1334368357862843 1103 7 0.07237833105953229 1103 8 0.040358769863225064 1103 9 0.0956067668247158 1103 10-11 0.053693933577790176 1103 12-13 0.17020319400675277 1103 14-15 0.2193263695609673 1103 16-17 0.12873538748016955 1103 18-19 0.1667507721323318 1103 20-21 0.2140311281966163 1103 22-23 0.1253378450905842 1103 24-25 0.17944413851098062 1103 26-27 0.18911292921745826 1103 28-29 0.1516915232651428 1103 30-31 0.05939761957628775 1103 32-33 0.10544573110987443 1103 34-35 0.17000071970928587 1103 36-37 0.06895504446944756 1103 38-39 0.027203499550424226 1103 40-41 -0.02097111519037753 1103 42-43 -0.03558097344743061 1103 44-45 -0.04649076554165532 1103 46-47 0.05264895433693084 1103 48-49 0.019716716491231523 1103 50-51 0.08552345753734869 1103 52-53 0.04541328752812035 1103 54-55 0.16598358627281584 1103 56-57 0.10933710463362445 1103 58-59 0.019187310167588123 1103 60-61 0.045463142776533516 1103 62-63 0.003146361356385796 1103 64-65 0.056149031750969414 1103 66-67 0.08293254357608504 1103 68-69 -0.04167559720261238 1103 70-71 -0.07634795170574904 1103 72-73 -0.07431568958454449 1103 74-75 -0.31212346000118174 1103 76-77 -0.31305064974752383 1103 78-79 -0.29122002848031414 1103 80-81 -0.3887011492381198 1103 82-83 -0.4432965203424537 1103 84-85 -0.3410225458095191 1103 86-87 -0.3503531902170529 1103 88-89 -0.4091157736062314 1103 90-91 -0.34871180456834594 1103 92-93 -0.31760679703107897 1103 94-95 -0.3288558122473546 1103 96-97 -0.37784502988019497 1103 98-99 -0.4318692337439636 1103 100-101 -1.221065426616093 1104 1 0.03182279264328969 1104 2 0.17648958178931906 1104 3 0.05299300915620364 1104 4 0.10890957370390453 1104 5 0.0743030540420051 1104 6 0.030792075460439605 1104 7 0.08288796059765957 1104 8 0.07871499233300483 1104 9 0.0844461907408629 1104 10-11 0.1053876467123942 1104 12-13 0.12108392299314374 1104 14-15 0.16036147443065119 1104 16-17 0.11947695186895402 1104 18-19 0.07393366076485819 1104 20-21 0.07697277361187815 1104 22-23 0.03258080455138668 1104 24-25 0.11267633469277882 1104 26-27 0.17195325926594762 1104 28-29 0.13078802728121275 1104 30-31 0.08765264805001749 1104 32-33 0.18069235565935315 1104 34-35 0.09998064130379447 1104 36-37 0.15404975127694343 1104 38-39 -0.06389606239343237 1104 40-41 -0.03270766450998508 1104 42-43 -0.07935263485752131 1104 44-45 -0.08172430356133731 1104 46-47 -0.07654242625201846 1104 48-49 -0.026942492789231665 1104 50-51 0.05884302220667337 1104 52-53 0.05126005345146467 1104 54-55 0.07233216638125839 1104 56-57 0.16961103682855594 1104 58-59 0.11138205088207798 1104 60-61 0.16653474489932307 1104 62-63 0.054616614082505066 1104 64-65 0.11611193225324001 1104 66-67 0.14442602112856662 1104 68-69 0.10612122451285444 1104 70-71 0.09235751272701975 1104 72-73 0.11517558731692645 1104 74-75 -0.12374479931025206 1104 76-77 -0.06169840316768216 1104 78-79 0.1636993498618935 1104 80-81 0.025229951826364783 1104 82-83 -0.008477056226478652 1104 84-85 0.021804565584073288 1104 86-87 0.07154950948273253 1104 88-89 -0.089758970861471 1104 90-91 -0.07034350955206037 1104 92-93 -0.07964681157713116 1104 94-95 -0.07980922539960744 1104 96-97 -0.19955243025288283 1104 98-99 -0.32209194595568746 1104 100-101 -1.2868141772273667 1105 1 0.08509948842554138 1105 2 0.15985760838535867 1105 3 0.10605145436294805 1105 4 0.11328607208643149 1105 5 0.07169131730832845 1105 6 0.03686775853675783 1105 7 0.14302551807564612 1105 8 0.10684096290101763 1105 9 0.049540929811470846 1105 10-11 0.08150476686333263 1105 12-13 0.11735221620094194 1105 14-15 0.1368293803999876 1105 16-17 0.13191469011250234 1105 18-19 0.1620517126610892 1105 20-21 0.16019087471264726 1105 22-23 0.1407074614236734 1105 24-25 0.08745225343901097 1105 26-27 0.17395837281440407 1105 28-29 0.18631258766842507 1105 30-31 0.2227141304439897 1105 32-33 0.2589017441855148 1105 34-35 0.2792045678483035 1105 36-37 0.19831717671696225 1105 38-39 0.3460298458255906 1105 40-41 0.3174328533821935 1105 42-43 0.2768258339162202 1105 44-45 0.2804540930922883 1105 46-47 0.24483009214733187 1105 48-49 0.1042818653127 1105 50-51 0.23235920910012453 1105 52-53 0.23231111972402374 1105 54-55 0.261828628424567 1105 56-57 0.2309391577390869 1105 58-59 0.1912424228589984 1105 60-61 0.25945852615670617 1105 62-63 0.08346407655969301 1105 64-65 0.10444432337223475 1105 66-67 0.35221983496794707 1105 68-69 0.28061321394608996 1105 70-71 0.15792399874444385 1105 72-73 0.037347010421186155 1105 74-75 0.27171048505817197 1105 76-77 0.2380977702788556 1105 78-79 0.42044912332332274 1105 80-81 0.3709012766378912 1105 82-83 0.3535865444049229 1105 84-85 0.6089437614681081 1105 86-87 0.20769148489908318 1105 88-89 0.22988462783897035 1105 90-91 0.3094038403246948 1105 92-93 0.366136822857662 1105 94-95 0.1190208293767796 1105 96-97 0.22522244158851734 1105 98-99 0.1187594581181699 1105 100-101 -0.8928337927341197 1106 1 0.07468804604450696 1106 2 0.19738119785739627 1106 3 0.07828789960154836 1106 4 0.13345550806186424 1106 5 0.13891343974264458 1106 6 0.1278971692723303 1106 7 0.051402434100623395 1106 8 0.11058087103912584 1106 9 0.08905285809481 1106 10-11 0.10423682428518077 1106 12-13 0.07558674978838553 1106 14-15 0.16968318749000844 1106 16-17 0.10874962445853242 1106 18-19 0.06584530215645401 1106 20-21 0.1525027187853638 1106 22-23 0.17610333300363834 1106 24-25 0.14459111582578288 1106 26-27 0.24471162635369836 1106 28-29 0.16848474940880465 1106 30-31 0.23884478054534242 1106 32-33 0.14392639503800098 1106 34-35 0.13065839663246948 1106 36-37 0.2128900177408326 1106 38-39 0.27651321714312616 1106 40-41 0.24183107399537107 1106 42-43 0.2624853885563212 1106 44-45 0.25137795479091807 1106 46-47 0.1736500995186816 1106 48-49 0.24179609480881936 1106 50-51 0.19261605150495598 1106 52-53 0.3173123852160984 1106 54-55 0.32685042754606997 1106 56-57 0.32600298052820875 1106 58-59 0.2781359489048043 1106 60-61 0.24122013194367753 1106 62-63 0.23134469808542235 1106 64-65 0.35590197195966056 1106 66-67 0.4685385301931362 1106 68-69 0.42696299828758555 1106 70-71 0.45043811743710194 1106 72-73 0.43894085977981234 1106 74-75 0.3255045410943964 1106 76-77 0.3083293728233727 1106 78-79 0.5316587587916253 1106 80-81 0.4869852434948285 1106 82-83 0.6219676709611299 1106 84-85 0.7382089654514381 1106 86-87 0.7056372471918486 1106 88-89 0.75089996070421 1106 90-91 0.7287443206751618 1106 92-93 0.8051813646795516 1106 94-95 0.9247400954147054 1106 96-97 1.0839723861867903 1106 98-99 1.0139494510500775 1106 100-101 0.707244177906464 1107 1 -0.04675591273682045 1107 2 0.12614471755373557 1107 3 0.030653765571194924 1107 4 0.019401825535744877 1107 5 0.05250840881982555 1107 6 -0.031933956547277376 1107 7 0.01788837460776449 1107 8 0.06167713780337891 1107 9 -0.003660102336269233 1107 10-11 -0.023766409124178267 1107 12-13 -0.011448743639739689 1107 14-15 0.0021844865539364378 1107 16-17 0.008540576364673313 1107 18-19 0.05753700695516528 1107 20-21 0.09096116567491919 1107 22-23 0.09881026831149597 1107 24-25 0.09170826784806962 1107 26-27 0.1210359900189033 1107 28-29 0.026459129417290228 1107 30-31 0.11145369745234035 1107 32-33 0.022908986342166315 1107 34-35 0.11975899553743119 1107 36-37 0.14304120207133053 1107 38-39 0.11594168684167983 1107 40-41 0.10489837665950574 1107 42-43 0.07645234290334457 1107 44-45 0.0735004421314116 1107 46-47 0.09474776516895389 1107 48-49 0.013915566833986759 1107 50-51 -0.02109841493155784 1107 52-53 0.07008041714594526 1107 54-55 0.10092007885170062 1107 56-57 0.13977924027601318 1107 58-59 0.24500988752069475 1107 60-61 0.12939082963256965 1107 62-63 0.21373346668296023 1107 64-65 0.3596223754005976 1107 66-67 0.4254566961874886 1107 68-69 0.3493793480707694 1107 70-71 0.264073042884462 1107 72-73 0.33202831135921684 1107 74-75 0.31128939462855243 1107 76-77 0.1540033315964351 1107 78-79 0.33482620296905097 1107 80-81 0.3501483753582839 1107 82-83 0.3866288500292754 1107 84-85 0.41814591093976716 1107 86-87 0.5867076345648634 1107 88-89 0.7276458902350527 1107 90-91 0.631507836516306 1107 92-93 0.7575038596753672 1107 94-95 0.7604510530705895 1107 96-97 0.8251732417102282 1107 98-99 0.9456329387703057 1107 100-101 1.6375132211372723 1108 1 0.12542633130063052 1108 2 0.09794491692046137 1108 3 0.13639887784496096 1108 4 0.05590981400507644 1108 5 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0.24049816084831832 2316 82-83 0.33748449601697317 2316 84-85 0.24725963699322762 2316 86-87 0.3511002453351679 2316 88-89 0.34014304411177676 2316 90-91 0.46688316978816147 2316 92-93 0.38799340920035874 2316 94-95 0.4949287659871331 2316 96-97 0.6938114931173764 2316 98-99 0.8141261297009059 2316 100-101 1.3155143272149097 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 31.0 5 50.0 6 86.0 7 225.0 8 662.0 9 1327.0 10 2196.0 11 3346.0 12 5046.0 13 7091.0 14 8715.0 15 10701.0 16 13607.0 17 18024.0 18 18188.0 19 13973.0 20 12985.0 21 12962.0 22 13876.0 23 15528.0 24 17662.0 25 21004.0 26 25639.0 27 31112.0 28 38234.0 29 47528.0 30 59118.0 31 75616.0 32 98319.0 33 132838.0 34 190893.0 35 290857.0 36 465369.0 37 684501.0 38 302450.0 39 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.321236789467065 23.220814749998866 14.023940844715863 24.434007615818214 2 13.659256340952009 21.860454544593594 41.20869692039314 23.27159219406126 3 16.70547661610577 29.281301862612402 30.29032266618481 23.72289885509702 4 11.44414303123699 18.24082776375518 40.03804094800073 30.276988257007098 5 12.178141645519128 38.47666497334823 35.53756420499078 13.807629176141864 6 27.918085815257065 39.68070151114465 19.325915310591345 13.075297363006941 7 25.469554930000033 32.23498714100797 25.106457452817217 17.189000476174783 8 25.46133456979673 35.30447721729064 20.79967057948236 18.43451763343027 9 26.253746987919858 15.046630656185517 22.554130314301872 36.145492041592746 10-11 24.669547202103807 26.61072654725552 28.783591251869943 19.936134998770736 12-13 24.948565925962964 24.64541646731346 28.866116849579154 21.53990075714442 14-15 22.228312792495743 25.777810440407578 26.80692951160361 25.18694725549307 16-17 21.028476918781706 27.775896312846456 28.079348826249994 23.116277942121847 18-19 20.837703951114236 28.188126542027824 29.888585707862035 21.085583798995906 20-21 21.838333926184195 27.14289069534777 29.014708383668836 22.004066994799203 22-23 22.08342945851313 27.30475981584878 28.482979876785514 22.12883084885258 24-25 21.81427895508236 27.431076825516605 29.254443635035702 21.500200584365327 26-27 21.77382114541817 27.272105666101094 28.836019724318668 22.11805346416207 28-29 21.206029122804225 28.06283234215488 28.783685956480582 21.947452578560316 30-31 22.30962195055889 27.298225197714814 28.384468140800756 22.007684710925542 32-33 22.48385949320911 27.726668856237264 28.01263889851719 21.77683275203643 34-35 22.362258773151016 27.188557258597 28.984005148900273 21.465178819351713 36-37 22.43987867202922 27.036158599159705 28.87518955127819 21.648773177532885 38-39 22.430338997433125 26.946030432792984 29.272300309419258 21.351330260354633 40-41 21.811643231047 26.68631904819992 29.506416225394116 21.995621495358968 42-43 22.090800432635575 26.92209339548839 28.637103235864046 22.350002936011986 44-45 21.86386628921197 27.807982234930318 28.446082960481284 21.882068515376428 46-47 22.31167179618503 27.20098765563048 28.18968503702856 22.29765551115592 48-49 22.7876831565922 26.712777771358905 28.372250981946056 22.12728809010284 50-51 22.223251345657815 27.169199636182768 27.636263834923046 22.971285183236375 52-53 21.63884813433811 27.721289634353074 27.661606788729543 22.978255442579268 54-55 21.948115510834775 27.76856617598314 27.434467250577416 22.848851062604673 56-57 22.903287272798902 26.98808653880029 27.549779584489205 22.558846603911604 58-59 22.321270617667256 27.10192148078614 28.211310230711796 22.365497670834806 60-61 22.347143917293327 28.19000169331844 27.270078987433454 22.19277540195478 62-63 22.500773736668904 27.32510236621364 26.921812252276418 23.252311644841043 64-65 21.492813624735632 27.729945635765308 27.303225601156456 23.4740151383426 66-67 22.32541268503348 26.68652984685905 27.37410146890301 23.61395599920446 68-69 21.681457753107342 27.20286983256721 27.306313673320904 23.809358741004544 70-71 22.029194793525072 26.77363759429628 27.73208008405337 23.46508752812528 72-73 22.703403721588145 26.77897875851347 27.92732898525525 22.590288534643136 74-75 22.415342015737274 27.76352681952895 27.208822238641915 22.612308926091856 76-77 22.16822051788222 27.3538047335496 27.457394814586255 23.02057993398192 78-79 21.768660192416643 27.459765766991023 27.638199426322522 23.133374614269805 80-81 21.588738754523526 27.57764457522666 28.130932909290827 22.702683760958983 82-83 22.35772565621552 26.61219000535106 28.750493671065904 22.279590667367515 84-85 22.782906157749537 27.30805984341157 27.129366817913624 22.779667180925266 86-87 21.65628415890512 27.886622219722014 27.643628163217603 22.813465458155264 88-89 22.177035495666885 26.913857064982892 27.4807209824126 23.428386456937623 90-91 21.999942040767312 26.58266113566381 28.188112940194216 23.22928388337466 92-93 22.291952835141956 27.11020917794862 27.491796608936713 23.106041377972712 94-95 20.881662798794103 27.16182254458282 28.093862394656348 23.862652261966726 96-97 21.268728171105387 27.32690168395615 27.489097746283537 23.915272398654928 98-99 20.838196415089552 27.99871732075353 27.228408958753114 23.934677305403806 100-101 19.598978021089252 28.084210031856816 27.375514744502432 24.9412972025515 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 144.0 1 101.5 2 98.5 3 131.5 4 101.5 5 94.0 6 154.5 7 303.5 8 499.5 9 606.5 10 630.5 11 681.5 12 793.5 13 978.0 14 1178.0 15 1385.0 16 1640.0 17 1887.5 18 2155.0 19 2423.0 20 2691.0 21 2965.5 22 3282.0 23 3736.5 24 4287.0 25 5042.5 26 6443.0 27 8514.5 28 12055.0 29 20006.0 30 30425.5 31 39832.0 32 52538.0 33 65416.0 34 78214.0 35 89362.0 36 95462.0 37 107767.0 38 108499.5 39 92359.5 40 79677.5 41 71239.0 42 68339.5 43 70136.0 44 74031.0 45 83909.0 46 93551.0 47 101735.5 48 111187.5 49 109437.5 50 103049.5 51 100645.5 52 95993.5 53 91556.5 54 88376.5 55 79984.0 56 69849.5 57 58725.5 58 48929.5 59 45363.5 60 38130.5 61 28140.0 62 21165.5 63 16708.5 64 12520.0 65 8096.5 66 5535.0 67 4023.0 68 3173.5 69 2513.0 70 1968.0 71 1567.0 72 1212.0 73 938.0 74 755.5 75 618.5 76 475.0 77 367.0 78 288.0 79 220.5 80 169.5 81 142.0 82 118.5 83 99.0 84 73.5 85 65.5 86 52.0 87 36.5 88 33.5 89 21.5 90 8.5 91 6.0 92 6.5 93 4.0 94 2.0 95 0.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08027162797604503 2 7.576368850971688E-5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.894092212742922E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.1364553276457532E-4 40-41 0.0027653746306046663 42-43 0.005758040326738483 44-45 0.0 46-47 1.894092212742922E-5 48-49 1.3258645489200457E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0023486743438012234 68-69 0.007216491330550533 70-71 0.0013448054710474746 72-73 0.0 74-75 1.3258645489200457E-4 76-77 0.0021024423561446437 78-79 0.005701217560356195 80-81 0.004337471167181291 82-83 0.004716289609729876 84-85 0.0024433789544383694 86-87 3.9775936467601364E-4 88-89 0.0 90-91 1.894092212742922E-5 92-93 9.47046106371461E-4 94-95 0.003523011515701835 96-97 0.0030494884625161048 98-99 0.0 100-101 2.4623198765657986E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2639787.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.61095503648063 #Duplication Level Percentage of deduplicated Percentage of total 1 81.10166704384541 29.69209485525848 2 10.869122572453305 7.958579155721693 3 3.047778851232304 3.347462834508074 4 1.3183755634015009 1.930679538915487 5 0.7105422761956163 1.3006815662658155 6 0.4415018838772914 0.9698283371491805 7 0.3266732284135032 0.8371873213948113 8 0.2329386631615754 0.6822485538613076 9 0.17961726312907783 0.5918363589774942 >10 1.2961383361102234 9.563598373457722 >50 0.2013763544407796 5.237447198491969 >100 0.2268751320782686 17.946258839365765 >500 0.031768821223443204 8.040934447536067 >1k 0.014894889950663532 9.842651367970175 >5k 7.291204870954177E-4 2.0585112511260757 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 9884 0.3744241486150208 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 9438 0.35752884607735397 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 8983 0.3402926069413934 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 7635 0.28922788088584417 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 6604 0.25017169945908513 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5907 0.2237680540134488 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 5530 0.20948659872936717 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 4957 0.1877803019713333 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 4448 0.16849844324561036 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4291 0.16255099369759757 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 4269 0.16171759312399067 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 4087 0.15482309746960646 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 4037 0.1529290052568635 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 4035 0.1528532415683538 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4034 0.15281535972409896 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 3673 0.13914001394809505 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 3584 0.13576852980941267 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 3532 0.13379867390816003 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3269 0.12383574886913225 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 3108 0.11773677194410004 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 3025 0.11459257887094679 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 3012 0.11410011489563363 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 2873 0.10883453854420831 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 2668 0.10106876047196232 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.788184425485844E-5 0.0 5 0.0 0.0 0.0 3.788184425485844E-5 0.0 6 0.0 0.0 0.0 3.788184425485844E-5 0.0 7 0.0 0.0 0.0 3.788184425485844E-5 0.0 8 0.0 0.0 0.0 3.788184425485844E-5 0.0 9 0.0 0.0 0.0 3.788184425485844E-5 0.0 10-11 0.0 0.0 0.0 3.788184425485844E-5 0.0 12-13 0.0 0.0 0.0 3.788184425485844E-5 3.788184425485844E-5 14-15 0.0 0.0 0.0 3.788184425485844E-5 3.788184425485844E-5 16-17 0.0 0.0 0.0 3.788184425485844E-5 3.788184425485844E-5 18-19 0.0 0.0 0.0 3.788184425485844E-5 9.47046106371461E-5 20-21 0.0 0.0 0.0 5.682276638228766E-5 1.5152737701943375E-4 22-23 0.0 0.0 0.0 9.47046106371461E-5 1.894092212742922E-4 24-25 0.0 0.0 0.0 3.0305475403886756E-4 2.2729106552915064E-4 26-27 0.0 0.0 0.0 6.81873196587452E-4 2.2729106552915064E-4 28-29 0.0 0.0 0.0 0.0020077377455074976 2.2729106552915064E-4 30-31 0.0 0.0 0.0 0.007746837150118552 2.2729106552915064E-4 32-33 0.0 0.0 0.0 0.023429920671629946 2.2729106552915064E-4 34-35 0.0 0.0 0.0 0.04858346525685595 2.4623198765657986E-4 36-37 0.0 0.0 0.0 0.08928750690870135 3.030547540388675E-4 38-39 0.0 0.0 0.0 0.14440559029952038 3.40936598293726E-4 40-41 0.0 0.0 0.0 0.2027057486077475 3.40936598293726E-4 42-43 0.0 0.0 0.0 0.2651539688618817 3.40936598293726E-4 44-45 0.0 3.788184425485844E-5 0.0 0.3385689830277973 3.40936598293726E-4 46-47 0.0 3.788184425485844E-5 0.0 0.4263033343220495 3.40936598293726E-4 48-49 0.0 3.788184425485844E-5 0.0 0.5209511221928133 3.788184425485844E-4 50-51 0.0 3.788184425485844E-5 0.0 0.6177202933418491 3.788184425485844E-4 52-53 0.0 3.788184425485844E-5 0.0 0.716080501949589 3.788184425485844E-4 54-55 0.0 3.788184425485844E-5 0.0 0.8249150404937974 3.788184425485844E-4 56-57 0.0 3.788184425485844E-5 0.0 0.9543194204683938 3.9775936467601364E-4 58-59 0.0 3.788184425485844E-5 0.0 1.1049755150699658 4.1670028680344286E-4 60-61 0.0 3.788184425485844E-5 0.0 1.2601206082157388 4.1670028680344286E-4 62-63 0.0 3.788184425485844E-5 0.0 1.4192243540861442 4.3564120893087207E-4 64-65 0.0 3.788184425485844E-5 0.0 1.5857908232747566 4.924639753131597E-4 66-67 0.0 3.788184425485844E-5 0.0 1.7686086036487034 4.924639753131597E-4 68-69 0.0 3.788184425485844E-5 0.0 1.9798567081359217 5.114048974405889E-4 70-71 0.0 3.788184425485844E-5 0.0 2.2171864623926094 5.492867416954474E-4 72-73 0.0 3.788184425485844E-5 0.0 2.462130467344524 6.06109508077735E-4 74-75 0.0 3.788184425485844E-5 0.0 2.707642699960262 6.629322744600227E-4 76-77 0.0 3.788184425485844E-5 0.0 2.977475076587619 6.81873196587452E-4 78-79 0.0 3.788184425485844E-5 0.0 3.2704532600546936 7.008141187148811E-4 80-81 0.0 3.788184425485844E-5 0.0 3.6026959750919296 7.197550408423104E-4 82-83 0.0 3.788184425485844E-5 0.0 3.9528757433838413 7.576368850971689E-4 84-85 0.0 3.788184425485844E-5 0.0 4.294361628419263 7.76577807224598E-4 86-87 0.0 3.788184425485844E-5 0.0 4.651871533574489 7.955187293520273E-4 88-89 0.0 3.788184425485844E-5 0.0 5.047623160504996 7.955187293520273E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3395 0.0 55.682995 2 ACCTGGG 3605 0.0 50.99 3 GTACCTG 3865 0.0 49.201305 1 CCTGGGG 3275 0.0 47.425922 4 CTGGGGG 2935 0.0 46.122845 5 GTACACG 430 0.0 35.37917 1 GTACCCG 295 0.0 33.84258 1 TGGGGGG 6490 0.0 32.641457 6 TATCACG 200 0.0 30.873985 2 GTACTAG 1925 0.0 28.647938 1 AGGGCTA 3715 0.0 27.616858 5 ATTTAGG 3955 0.0 26.805536 1 CTATTGA 3895 0.0 26.462543 9 CTGTGCG 2420 0.0 26.300787 9 GCTATTG 3875 0.0 26.231396 8 GGGGGGA 6910 0.0 26.189444 7 TAGTACT 2495 0.0 25.510183 4 TAGGCAT 2475 0.0 24.756762 5 GGGGAAT 7425 0.0 24.436907 9 TTTAGGG 4330 0.0 24.352547 2 >>END_MODULE