FastQCFastQC Report
Sat 18 Jun 2016
SRR1931058_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931058_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1968191
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA56420.2866591707816975No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA55690.28295018115619874No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC49120.24956927452671004No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC44770.22746776100490249No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC44630.22675644792603972No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC42770.21730614559257716No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT40250.20450251017304724No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38790.19708453092204975No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT32590.16558352314384123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30090.15288150387843455No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC28040.14246584808080112No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC26870.1365213030645908No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC24130.1225998899497051No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA23710.12046595071311676No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT22880.11624888031700176No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC22670.1151819106987076No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG22620.11492787031339947No Hit
GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA21830.11091403222553096No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC21180.10761150721652522No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA21160.10750989106240198No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC20620.10476625490107414No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG20330.10329282066628695No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT20080.1020226187397463No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA19880.10100645719851375No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT19800.10059999258202074No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG19790.10054918450495912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG25750.060.5183032
ACCTGGG28100.055.961433
GTACCTG29900.054.1780051
CCTGGGG27550.048.4824524
CTGGGGG25300.047.3649445
GTACACG3250.036.43551
GTACCCG2500.034.103631
TGGGGGG65650.031.383446
GTACTAG15500.030.2532181
CTGTGCG20750.029.2186369
TAGTACT21150.028.8892574
TATTCTC10800.028.506685
GGGGGGA65600.027.8695187
GTACTGT22550.027.5150786
ACTGTGC23800.027.463718
TATCACG1458.0891004E-726.1330492
GGGGAAT70150.025.9281569
TAGGCAT20550.025.8144725
TCTAACG9400.025.6986562
ATAACGC3200.025.162633
Produced by FastQC (version 0.11.5)