##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931058_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1968191 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.465839443428 33.0 33.0 33.0 27.0 33.0 2 30.358419482661997 33.0 33.0 33.0 27.0 33.0 3 30.390214669206394 33.0 33.0 33.0 27.0 33.0 4 33.90802772698381 37.0 37.0 37.0 27.0 37.0 5 33.85734667011484 37.0 37.0 37.0 27.0 37.0 6 33.963455274411885 37.0 37.0 37.0 27.0 37.0 7 33.921577224974605 37.0 37.0 37.0 27.0 37.0 8 33.916294201121744 37.0 37.0 37.0 27.0 37.0 9 33.93901252469907 37.0 37.0 37.0 27.0 37.0 10-11 33.814101629364224 37.0 37.0 37.0 27.0 37.0 12-13 33.681145529067045 37.0 37.0 37.0 27.0 37.0 14-15 35.11778633272888 40.0 37.0 40.0 27.0 40.0 16-17 35.036087198854176 38.5 37.0 40.0 27.0 40.0 18-19 34.96790606196248 38.5 37.0 40.0 27.0 40.0 20-21 34.873747771430715 37.0 37.0 40.0 27.0 40.0 22-23 34.74638716466034 37.0 37.0 40.0 24.5 40.0 24-25 34.59079987663799 37.0 37.0 40.0 22.0 40.0 26-27 34.375633767251244 37.0 35.0 40.0 22.0 40.0 28-29 34.21917232626305 37.0 33.0 40.0 22.0 40.0 30-31 34.04411055634336 37.0 33.0 40.0 18.5 40.0 32-33 33.846415058294646 37.0 33.0 40.0 15.0 40.0 34-35 33.67388835737995 37.0 33.0 40.0 15.0 40.0 36-37 33.40814738000529 37.0 33.0 40.0 15.0 40.0 38-39 33.16138677597855 37.0 33.0 40.0 10.5 40.0 40-41 32.88005381591522 37.0 33.0 40.0 6.0 40.0 42-43 32.630021171725716 37.0 33.0 40.0 6.0 40.0 44-45 32.2718663991452 37.0 33.0 40.0 6.0 40.0 46-47 32.05019101296571 37.0 33.0 40.0 2.0 40.0 48-49 31.951439672267583 37.0 33.0 40.0 2.0 40.0 50-51 31.11950085128933 37.0 30.0 38.5 2.0 40.0 52-53 31.239192486908028 37.0 33.0 38.5 2.0 40.0 54-55 31.65070844242251 37.0 33.0 40.0 2.0 40.0 56-57 31.53352062884141 37.0 33.0 40.0 2.0 40.0 58-59 31.318609067920747 37.0 33.0 40.0 2.0 40.0 60-61 31.30275415343328 37.0 33.0 40.0 2.0 40.0 62-63 31.079896971381334 37.0 33.0 40.0 2.0 40.0 64-65 30.815361923715738 37.0 33.0 37.0 2.0 40.0 66-67 30.537570286623605 37.0 33.0 37.0 2.0 40.0 68-69 30.26669566114264 37.0 27.0 37.0 2.0 40.0 70-71 30.02775289593337 37.0 27.0 37.0 2.0 40.0 72-73 29.452010755053752 37.0 27.0 37.0 2.0 40.0 74-75 29.280934624739167 37.0 27.0 37.0 2.0 38.5 76-77 28.950686950605913 33.0 27.0 37.0 2.0 37.0 78-79 28.702692980508495 33.0 27.0 37.0 2.0 37.0 80-81 28.487138697413005 33.0 27.0 37.0 2.0 37.0 82-83 28.224667219797265 33.0 27.0 37.0 2.0 37.0 84-85 27.850837901402862 33.0 27.0 37.0 2.0 37.0 86-87 27.67953237262034 33.0 27.0 37.0 2.0 37.0 88-89 27.498227052150934 33.0 27.0 37.0 2.0 37.0 90-91 27.188365864898273 33.0 24.5 37.0 2.0 37.0 92-93 26.879475619998264 33.0 22.0 37.0 2.0 37.0 94-95 26.60917080710155 33.0 22.0 37.0 2.0 37.0 96-97 26.28580584912745 33.0 22.0 37.0 2.0 37.0 98-99 25.93199313481263 33.0 15.0 37.0 2.0 37.0 100-101 25.217342727408063 33.0 8.5 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2474747856702244 1101 2 0.23517665062666637 1101 3 0.1843978330826559 1101 4 0.24313250923545837 1101 5 0.04922812439127 1101 6 0.2719283506932584 1101 7 0.2618840256281558 1101 8 0.1408427989626162 1101 9 0.31584798571807937 1101 10-11 0.1319640493627432 1101 12-13 0.20695045687858027 1101 14-15 0.3894503981670212 1101 16-17 0.21641347683546996 1101 18-19 0.24761921833934508 1101 20-21 0.2917729648455989 1101 22-23 0.3314451535507317 1101 24-25 0.23653847526214378 1101 26-27 0.2195019977065371 1101 28-29 0.23376882345890948 1101 30-31 0.1576555864924174 1101 32-33 0.3099337218000997 1101 34-35 0.31278959816300755 1101 36-37 0.34667945958415913 1101 38-39 0.43987924820574165 1101 40-41 0.32225971990507674 1101 42-43 0.32616450242203143 1101 44-45 0.12953846734836816 1101 46-47 -0.034721709705280546 1101 48-49 -0.21335435352057175 1101 50-51 -0.31400788396333823 1101 52-53 -0.164330655475748 1101 54-55 0.08502769513749442 1101 56-57 0.07573703627019768 1101 58-59 0.0776628784830713 1101 60-61 0.07814731739021497 1101 62-63 0.09398529901562824 1101 64-65 0.13526202827911504 1101 66-67 0.0035998287952310193 1101 68-69 0.10913525656545531 1101 70-71 0.062458216963651836 1101 72-73 -0.07653551593055141 1101 74-75 -0.11055320075787378 1101 76-77 -0.11520566895108786 1101 78-79 -0.1645850762732728 1101 80-81 0.07860070879052117 1101 82-83 -0.12183283053475691 1101 84-85 -0.006276524837957709 1101 86-87 0.07912251628066613 1101 88-89 -0.1547044351595126 1101 90-91 -0.1871969248928842 1101 92-93 -0.3500393223226368 1101 94-95 -0.16621332805670264 1101 96-97 -0.16876934851884684 1101 98-99 -0.05851418153173782 1101 100-101 -0.08051656275306485 1102 1 0.017081509063139322 1102 2 -0.10018017270343194 1102 3 -0.030223452585403265 1102 4 -0.012221585650927125 1102 5 0.03411127720315221 1102 6 -0.006779636032540282 1102 7 0.04168338983731701 1102 8 0.04592024234740677 1102 9 -0.15771402926932154 1102 10-11 -0.1714725206662493 1102 12-13 -0.053269785635649214 1102 14-15 0.049819911932971195 1102 16-17 0.08402764316900857 1102 18-19 0.061656268066819564 1102 20-21 0.031826677115340374 1102 22-23 0.06548058524762723 1102 24-25 0.0729958766415919 1102 26-27 0.11506732489770144 1102 28-29 0.14589834204722507 1102 30-31 0.3013205209145866 1102 32-33 0.07055625113087416 1102 34-35 0.09500823533710445 1102 36-37 0.23831900044418575 1102 38-39 0.12683062480077467 1102 40-41 0.07397625998464008 1102 42-43 0.2559175465403314 1102 44-45 0.20748153805313052 1102 46-47 0.10375077425717905 1102 48-49 -0.020729241686105127 1102 50-51 0.15285611898083928 1102 52-53 0.27600700529621136 1102 54-55 0.32843279288557525 1102 56-57 0.42940108236185637 1102 58-59 0.3307055691092806 1102 60-61 0.28761311349298424 1102 62-63 0.3069174876716474 1102 64-65 0.2099568232968494 1102 66-67 0.2694530385237215 1102 68-69 0.21189717129841767 1102 70-71 0.2754552854729688 1102 72-73 0.1541066518496983 1102 74-75 0.1894429921113705 1102 76-77 0.15190373529640055 1102 78-79 0.04365180974757976 1102 80-81 0.2070926408459286 1102 82-83 0.20535103484509776 1102 84-85 0.009933920659793927 1102 86-87 -0.04133129370543642 1102 88-89 0.3017600728640133 1102 90-91 0.2350824042906723 1102 92-93 0.23294898978593892 1102 94-95 -0.05765045646487721 1102 96-97 0.11438885679971378 1102 98-99 0.2586923045500278 1102 100-101 0.12667193463587623 1103 1 -0.0831424158797347 1103 2 -0.0435225725411037 1103 3 0.010267829560024921 1103 4 -0.06247063270173925 1103 5 -0.09058375490000259 1103 6 -0.07727009687828712 1103 7 8.164838664654894E-4 1103 8 0.07926138607968625 1103 9 -0.04803549562743825 1103 10-11 -0.11547014855951687 1103 12-13 -0.17487648937353129 1103 14-15 0.00909492171609827 1103 16-17 0.016237808926611308 1103 18-19 -0.03831885138396984 1103 20-21 -0.07870153935992619 1103 22-23 -0.07996615346795011 1103 24-25 -0.1767637724218858 1103 26-27 -0.11360545481523587 1103 28-29 0.038440940929284295 1103 30-31 0.0013408118479176778 1103 32-33 -0.18506723610757803 1103 34-35 -0.2986799558228199 1103 36-37 -0.1394293340083621 1103 38-39 -0.0392150346304021 1103 40-41 -0.06007615342863204 1103 42-43 -0.2100909128463968 1103 44-45 -0.2466488746741149 1103 46-47 -0.456134654437097 1103 48-49 -0.48164745473124526 1103 50-51 -0.520717718276213 1103 52-53 -0.26832868403993615 1103 54-55 -0.12441433908762178 1103 56-57 -0.017656490745892484 1103 58-59 -0.12903444122943242 1103 60-61 -0.05962514554493836 1103 62-63 -0.1452291239840413 1103 64-65 -0.015047283201564454 1103 66-67 -0.062103831648123275 1103 68-69 -0.1383466822396926 1103 70-71 0.0014866204714429898 1103 72-73 -0.14083414210553258 1103 74-75 -0.022739176989404086 1103 76-77 -0.1774730673356224 1103 78-79 -0.20726249004612995 1103 80-81 -0.06574794488568259 1103 82-83 -0.05070725810766419 1103 84-85 0.089447807871192 1103 86-87 0.10988386748670464 1103 88-89 -0.07967384391417198 1103 90-91 -0.017380286963451397 1103 92-93 -0.10699419560072343 1103 94-95 -0.058545431560173 1103 96-97 -0.062476822052783376 1103 98-99 0.06108337364065264 1103 100-101 0.050790486829473735 1104 1 0.07371949350748608 1104 2 -0.038587480221231374 1104 3 0.11315272750455918 1104 4 0.1644095813915385 1104 5 0.07944699969804248 1104 6 0.20827714877939485 1104 7 0.1760391799074057 1104 8 0.14797974260421398 1104 9 0.03390217864301093 1104 10-11 0.04017901797068646 1104 12-13 0.11771111412320323 1104 14-15 0.1007681871993853 1104 16-17 0.0995329139879928 1104 18-19 0.24410554636651938 1104 20-21 0.17671921090869347 1104 22-23 0.2110217510694099 1104 24-25 0.27989826572638066 1104 26-27 0.30214915444883417 1104 28-29 0.2240082634398206 1104 30-31 0.3131651808851501 1104 32-33 0.2516250631295023 1104 34-35 0.24213830491605393 1104 36-37 0.31587443214004196 1104 38-39 0.24880396323180776 1104 40-41 0.27763894369174125 1104 42-43 0.1246578012255668 1104 44-45 0.211839147632503 1104 46-47 0.016860856872114027 1104 48-49 0.10346522239931488 1104 50-51 0.213021993738721 1104 52-53 0.3407455487214506 1104 54-55 0.4075659003540828 1104 56-57 0.44280556848868 1104 58-59 0.5136816989900019 1104 60-61 0.42973636567006324 1104 62-63 0.45131109589675233 1104 64-65 0.3413959284667918 1104 66-67 0.36585363458574705 1104 68-69 0.35001491197458634 1104 70-71 0.3129708607027659 1104 72-73 0.34250964091191705 1104 74-75 0.2519707741756143 1104 76-77 0.0983212021249038 1104 78-79 0.12238905878772854 1104 80-81 0.20219896698030126 1104 82-83 -0.0734070003612537 1104 84-85 0.10522496153426175 1104 86-87 0.14124609877653427 1104 88-89 0.09701115362949864 1104 90-91 0.14023702803227422 1104 92-93 0.11262386190207252 1104 94-95 0.09184814205233849 1104 96-97 0.21533074075187386 1104 98-99 0.45294708882302004 1104 100-101 0.12300182318517727 1105 1 0.0534727619452795 1105 2 0.03218842698353086 1105 3 -0.013190920173606457 1105 4 -0.09879376115428329 1105 5 -0.2575786242290832 1105 6 -0.20627641869538849 1105 7 -0.22555811521448987 1105 8 0.010602463684499241 1105 9 0.018311619667862544 1105 10-11 -0.03037026712774349 1105 12-13 0.09155722989844861 1105 14-15 0.05398856547596864 1105 16-17 -0.09507439157935238 1105 18-19 0.0691087373099748 1105 20-21 0.10617227017689146 1105 22-23 0.024537936316050946 1105 24-25 -0.07489700594664583 1105 26-27 0.026138667315500186 1105 28-29 0.16637878276912232 1105 30-31 0.07766678156658458 1105 32-33 0.03222842495123501 1105 34-35 0.2278153970679 1105 36-37 -0.014413351886481962 1105 38-39 0.11057255340786298 1105 40-41 0.11211741328921221 1105 42-43 -0.02719437864465135 1105 44-45 0.09686320343885058 1105 46-47 0.11982413627633548 1105 48-49 0.12272823168039793 1105 50-51 0.10940776187043966 1105 52-53 0.24311765701034815 1105 54-55 0.29487936040477436 1105 56-57 0.18382260682427898 1105 58-59 0.4161143700831751 1105 60-61 0.4325324135889126 1105 62-63 0.34708511458396174 1105 64-65 0.35886497631531356 1105 66-67 0.3979125875916196 1105 68-69 0.2404541941816234 1105 70-71 0.15746133866702294 1105 72-73 0.1838339215169711 1105 74-75 0.24135964989553216 1105 76-77 0.2083312568528406 1105 78-79 0.32686289174900196 1105 80-81 0.29335093663898704 1105 82-83 0.3321834023227872 1105 84-85 0.41608276527951915 1105 86-87 0.37197058620682455 1105 88-89 0.31339606216012683 1105 90-91 0.3580957617096274 1105 92-93 0.39995529543016417 1105 94-95 0.4540318153622458 1105 96-97 0.24071632393911813 1105 98-99 0.2683231382526863 1105 100-101 0.22107371188506875 1106 1 -0.09802043551979267 1106 2 -0.2525591047189941 1106 3 -0.041794278101878746 1106 4 -0.09876194089547141 1106 5 -0.26023548855895484 1106 6 -0.12053813141445602 1106 7 -0.27997126690929974 1106 8 -0.12798876436111328 1106 9 -0.19598840464281864 1106 10-11 -0.1883411783199449 1106 12-13 -0.20592922421737114 1106 14-15 -0.2816104127118777 1106 16-17 -0.27424268638259264 1106 18-19 -0.22626065134648599 1106 20-21 -0.2027664223668566 1106 22-23 -0.16351546571509346 1106 24-25 -0.17321320513128313 1106 26-27 -0.3041794207196986 1106 28-29 -0.243857794491106 1106 30-31 -0.2949691959454057 1106 32-33 -0.18072445688232364 1106 34-35 -0.28722782120762247 1106 36-37 -0.3167480679161301 1106 38-39 -0.32709117773386254 1106 40-41 -0.2564068776599555 1106 42-43 -0.17706614300163892 1106 44-45 -0.11914824167184435 1106 46-47 -0.12760510501414046 1106 48-49 -0.22019627921040552 1106 50-51 -0.2004403536701922 1106 52-53 -0.18979187290260313 1106 54-55 -0.09218863185144599 1106 56-57 -0.0788993235708162 1106 58-59 -0.08556828520286786 1106 60-61 -0.3359577660428137 1106 62-63 -0.1904220608844014 1106 64-65 -0.11874018009398668 1106 66-67 4.300791878861787E-4 1106 68-69 -0.045882313964998644 1106 70-71 -0.10229519816874344 1106 72-73 -0.29157678256353137 1106 74-75 -0.2826262066358325 1106 76-77 -0.23086913822749366 1106 78-79 -0.05437257434778431 1106 80-81 0.050252050729888253 1106 82-83 0.0720305123432432 1106 84-85 0.1201549947214069 1106 86-87 0.14461445514299598 1106 88-89 0.2038345835108828 1106 90-91 0.09656505452346664 1106 92-93 0.14040953598877337 1106 94-95 0.21989911470051027 1106 96-97 0.23687625510553545 1106 98-99 -0.01064647085452819 1106 100-101 -0.010738019098827323 1107 1 -0.035877944241804016 1107 2 0.012421335338405726 1107 3 0.007533571591746835 1107 4 -0.024125648798687394 1107 5 0.003077615283217483 1107 6 -0.006119310663329713 1107 7 -0.03816978423532191 1107 8 -0.15196378169169833 1107 9 -0.17785042042542187 1107 10-11 -0.08698619945572972 1107 12-13 0.0010614053087110165 1107 14-15 0.163360956997046 1107 16-17 0.0016996076560005235 1107 18-19 0.04887978738907606 1107 20-21 0.16831144724059044 1107 22-23 -0.027512520194648005 1107 24-25 -0.05786947024390798 1107 26-27 0.028153288934696263 1107 28-29 -0.18095130268142157 1107 30-31 -0.09009128189867255 1107 32-33 -0.04750476184628383 1107 34-35 -0.0892243089753535 1107 36-37 -0.08302358505299878 1107 38-39 -0.2866344149111413 1107 40-41 -0.279064253014802 1107 42-43 -0.26030335974128604 1107 44-45 -0.11705044615123228 1107 46-47 -0.16751944718822998 1107 48-49 -0.4180683478392524 1107 50-51 -0.41709923976333485 1107 52-53 -0.26464308638847456 1107 54-55 -0.33563278965183585 1107 56-57 -0.2379840617847222 1107 58-59 -0.3153710384161492 1107 60-61 -0.31841177126257847 1107 62-63 -0.24373082684105185 1107 64-65 -0.40861845542567465 1107 66-67 -0.3738016970689557 1107 68-69 -0.16761179119184177 1107 70-71 -0.16068377850631066 1107 72-73 -0.28073268528145334 1107 74-75 -0.2521369626930259 1107 76-77 -0.2897682031036659 1107 78-79 -0.18958747477493887 1107 80-81 -0.2095014715517749 1107 82-83 -0.20418194392340183 1107 84-85 -0.08114940424963635 1107 86-87 -0.06433833391517751 1107 88-89 -0.09789248186147148 1107 90-91 -0.08484931317484978 1107 92-93 -0.17316755247868798 1107 94-95 -0.06692870724938516 1107 96-97 -0.0803046815768731 1107 98-99 -0.12169029829496836 1107 100-101 -0.11947073260035168 1108 1 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-0.922854087405721 2316 80-81 -0.8221347606674136 2316 82-83 -0.8437124193747003 2316 84-85 -0.7610928457335753 2316 86-87 -0.9035726153131414 2316 88-89 -0.8131591962574483 2316 90-91 -0.8495223136499916 2316 92-93 -0.8112389156808248 2316 94-95 -0.8471413248437294 2316 96-97 -0.9518297047069559 2316 98-99 -0.8034920295932864 2316 100-101 -0.6384617665132453 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 85041.0 3 12540.0 4 9422.0 5 7829.0 6 8178.0 7 8591.0 8 8687.0 9 9068.0 10 9301.0 11 9285.0 12 10097.0 13 11162.0 14 11434.0 15 12832.0 16 15238.0 17 19277.0 18 16623.0 19 12850.0 20 12195.0 21 12472.0 22 13235.0 23 14868.0 24 17130.0 25 19777.0 26 22525.0 27 26071.0 28 30917.0 29 37099.0 30 44871.0 31 55838.0 32 70866.0 33 92123.0 34 124469.0 35 177310.0 36 267087.0 37 393424.0 38 258220.0 39 239.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.44815146950109 20.983393329387226 12.135444584731617 23.433010616380066 2 15.628051798128348 20.328265506564193 41.085980299801534 22.957702395505926 3 18.73645328957062 28.41499042267261 28.85209253171696 23.996463756039812 4 12.409872089626177 16.903679804895397 39.776595069036034 30.909853036442392 5 12.84775553982736 38.07338540573745 34.529359192381506 14.549499862053688 6 28.225563474276633 38.18907819413867 19.506948258578564 14.078410073006125 7 26.303595535189423 32.0213841034737 24.14801205777285 17.527008303564035 8 26.041781514090857 34.186570307454915 20.835173009123604 18.93647516933062 9 27.062973054952494 14.975172633143835 22.11467281376655 35.84718149813712 10-11 25.540083645358514 25.913954975394915 27.885664522819308 20.660296856427262 12-13 25.80779147140483 24.268331749391127 28.011998838527063 21.911877940676984 14-15 23.167052315058985 25.209642327629396 26.22571659974981 25.397588757561806 16-17 21.50191440837782 27.325648600948682 27.701111959926866 23.471325030746637 18-19 21.066989342402533 27.697178001515653 29.712139946289025 21.52369270979279 20-21 22.352530826530558 27.052989267809885 28.665815462015626 21.928664443643935 22-23 22.74255903009413 26.957495486972554 27.934585616944695 22.365359865988616 24-25 22.228508310423127 26.979190535877866 29.033081647055596 21.759219506643408 26-27 22.341784918232023 26.90739872298979 28.320904830882775 22.429911527895413 28-29 21.639922141702712 27.335990257043143 28.82393019783141 22.200157403422736 30-31 22.394091833566964 27.15170936154062 28.522206432200942 21.93199237269147 32-33 22.815646448947284 27.473883378188397 27.636951901517687 22.073518271346632 34-35 22.520578541411883 27.02075154291428 28.723076164864082 21.735593750809752 36-37 22.651587168115288 26.658337529233698 28.343946293830225 22.34612900882079 38-39 22.8087365504669 26.738131614258982 28.703464247118294 21.749667588155823 40-41 22.420402553621738 25.953886448682418 29.077906802460134 22.547804195235713 42-43 22.543143054495996 26.358627378803018 28.267411106800893 22.830818459900097 44-45 22.29671300055178 27.49263532058584 28.176897617999998 22.033754060862382 46-47 22.51754851286413 26.95458229152832 27.672055520626138 22.855813674981405 48-49 23.197291871067648 26.597826203537668 27.687610095625185 22.5172718297695 50-51 22.677244806707648 26.86355801168105 27.232308422976644 23.226888758634658 52-53 22.124160439985655 27.50116810012243 27.19688853304213 23.177782926849787 54-55 22.350895222540146 27.375514288437884 27.204921672169576 23.0686688168524 56-57 23.13597902107702 26.669012492926214 27.14295942544413 23.052049060552637 58-59 22.355209777719345 26.997781728845016 27.803045243456914 22.843963249978724 60-61 22.51514276150781 27.834940460633405 27.03733762511979 22.61257915273899 62-63 22.821367383338163 27.0137759263382 26.68905511542261 23.475801574901027 64-65 21.813539139460396 27.284492584739017 27.27410039416458 23.627867881636007 66-67 22.616556611173706 26.507778930060937 27.210694297013323 23.664970161752034 68-69 22.200052656961287 26.880589391302845 27.10868468105108 23.81067327068479 70-71 22.17842341615068 26.628007478874622 27.44034955742522 23.753219547549477 72-73 22.977759749156316 26.48803990471464 27.639105923785028 22.895094422344016 74-75 23.03319794133331 27.173644326305617 26.985522318227044 22.807635414134026 76-77 22.939932298640606 26.714122037298633 26.940687944382834 23.405257719677923 78-79 22.298435184113533 27.00731192356972 27.18420836611318 23.510044526203565 80-81 22.010888676204836 27.13242946007129 27.882140756754332 22.974541106969546 82-83 22.89024639417259 26.060551710573847 28.649557955902083 22.39964393935148 84-85 23.134023234290947 26.747813203494797 27.157316116633563 22.960847445580693 86-87 22.034260036740108 27.365319588773612 27.789921759817076 22.8104986146692 88-89 22.9819000330072 26.30645861263809 27.498869197762033 23.212772156592678 90-91 22.717087669508505 26.205080358218563 27.840696202708976 23.237135769563956 92-93 23.077665349677645 26.45398796123683 27.58026054282896 22.888086146256562 94-95 21.710012403161546 26.56661500591651 28.296058095563648 23.427314495358296 96-97 22.1553517809248 26.73565050225341 27.558449514894594 23.550548201927192 98-99 21.695062054772876 27.379364234049685 27.346006788023992 23.579566923153454 100-101 22.22966285616876 26.840017606484885 26.958327078621096 23.97199245872526 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 90.0 1 65.5 2 69.0 3 98.5 4 83.0 5 76.5 6 129.0 7 220.5 8 333.0 9 392.0 10 392.5 11 416.5 12 480.5 13 575.5 14 746.5 15 983.0 16 1233.5 17 1480.0 18 1798.5 19 2152.5 20 2450.0 21 2734.0 22 3161.5 23 3691.0 24 4173.5 25 4874.5 26 6084.5 27 7500.0 28 9695.0 29 14792.5 30 21554.5 31 27287.5 32 34772.0 33 41864.0 34 47338.5 35 53464.5 36 57562.0 37 65141.5 38 67849.0 39 62229.5 40 57283.5 41 52382.0 42 50844.5 43 53901.0 44 60183.0 45 70042.5 46 78118.5 47 82724.5 48 87422.0 49 85296.0 50 79852.0 51 78882.0 52 76384.5 53 72816.5 54 69377.0 55 61811.0 56 54690.0 57 45642.5 58 36539.5 59 33600.5 60 28913.0 61 22126.0 62 17143.5 63 14261.0 64 11394.5 65 7655.5 66 5433.0 67 4174.0 68 3325.5 69 2740.0 70 2281.5 71 1889.0 72 1536.0 73 1309.5 74 1137.0 75 931.0 76 752.5 77 628.5 78 522.5 79 416.5 80 344.0 81 285.5 82 237.5 83 199.5 84 163.0 85 132.0 86 99.5 87 87.0 88 72.0 89 54.5 90 47.5 91 37.0 92 23.5 93 16.5 94 16.0 95 9.5 96 4.5 97 3.5 98 2.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.0484846236975986E-4 2 0.004521918858484771 3 5.0808077061626644E-5 4 8.129292329860263E-4 5 0.001727474620095306 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.5404038530813322E-5 12-13 2.5404038530813322E-5 14-15 0.003912221933745252 16-17 0.004547322897015584 18-19 0.003277120970474919 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 3.0484846236975986E-4 42-43 2.5404038530813322E-5 44-45 6.605050018011463E-4 46-47 0.7240405021667105 48-49 0.519969860648687 50-51 0.4025015864822063 52-53 0.3390168941937038 54-55 0.3302525009005732 56-57 0.2967445740784304 58-59 0.2973288669646391 60-61 0.2729409899750583 62-63 0.2679617984230189 64-65 0.26310962706363356 66-67 0.250941092607374 68-69 0.23056705370566172 70-71 0.24357392143343812 72-73 0.7997191329500034 74-75 0.529369354905088 76-77 0.622297327850803 78-79 0.34925472172162153 80-81 0.30327341198084945 82-83 0.2847284638533557 84-85 0.2766753836390879 86-87 0.25106811280002805 88-89 0.25317664799808554 90-91 0.2641765966819277 92-93 0.22192968060518514 94-95 0.1916734707149865 96-97 0.14978221117767535 98-99 0.1581401398543129 100-101 0.1527290796472497 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1968191.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.826066029495664 #Duplication Level Percentage of deduplicated Percentage of total 1 87.6617641018004 37.542084976857765 2 7.178473035055196 6.14851520380456 3 1.8084544408290941 2.3234696788284435 4 0.7438832905221195 1.274303796725552 5 0.4223324930001986 0.904341961581401 6 0.28578705772212926 0.7343481242629918 7 0.20589019748888326 0.617222703473937 8 0.1504977132214085 0.5156180002966514 9 0.12976560533241355 0.5001615344091074 >10 0.9782035185452141 8.75799797543889 >50 0.1847413097711421 5.629218148307217 >100 0.21609905094779683 19.89463843794343 >500 0.02337484059352009 6.741271177450035 >1k 0.010494826388927387 7.844216484496277 >5k 2.3851878156653155E-4 0.5725917961238908 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 5642 0.2866591707816975 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 5569 0.28295018115619874 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 4912 0.24956927452671004 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 4477 0.22746776100490249 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 4463 0.22675644792603972 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 4277 0.21730614559257716 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 4025 0.20450251017304724 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3879 0.19708453092204975 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 3259 0.16558352314384123 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3009 0.15288150387843455 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 2804 0.14246584808080112 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 2687 0.1365213030645908 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 2413 0.1225998899497051 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 2371 0.12046595071311676 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 2288 0.11624888031700176 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 2267 0.1151819106987076 No Hit GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG 2262 0.11492787031339947 No Hit GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 2183 0.11091403222553096 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 2118 0.10761150721652522 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 2116 0.10750989106240198 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 2062 0.10476625490107414 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 2033 0.10329282066628695 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 2008 0.1020226187397463 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 1988 0.10100645719851375 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 1980 0.10059999258202074 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 1979 0.10054918450495912 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 5.0808077061626644E-5 0.0 7 0.0 0.0 0.0 5.0808077061626644E-5 0.0 8 0.0 0.0 0.0 5.0808077061626644E-5 0.0 9 0.0 0.0 0.0 5.0808077061626644E-5 5.0808077061626644E-5 10-11 0.0 0.0 0.0 5.0808077061626644E-5 5.0808077061626644E-5 12-13 0.0 0.0 0.0 5.0808077061626644E-5 5.0808077061626644E-5 14-15 0.0 0.0 0.0 5.0808077061626644E-5 5.0808077061626644E-5 16-17 0.0 0.0 0.0 5.0808077061626644E-5 5.0808077061626644E-5 18-19 0.0 0.0 0.0 5.0808077061626644E-5 5.0808077061626644E-5 20-21 0.0 0.0 0.0 1.5242423118487993E-4 7.621211559243997E-5 22-23 0.0 0.0 0.0 2.0323230824650658E-4 1.5242423118487993E-4 24-25 0.0 0.0 0.0 3.0484846236975986E-4 1.5242423118487993E-4 26-27 0.0 0.0 0.0 6.859090403319597E-4 1.5242423118487993E-4 28-29 0.0 0.0 0.0 0.001956110966872626 1.5242423118487993E-4 30-31 0.0 0.0 0.0 0.00688449444185041 1.5242423118487993E-4 32-33 0.0 0.0 0.0 0.02050105909436635 1.5242423118487993E-4 34-35 0.0 0.0 0.0 0.042780400885889636 1.5242423118487993E-4 36-37 0.0 0.0 0.0 0.07834605482902827 2.0323230824650658E-4 38-39 0.0 0.0 0.0 0.1257245866889951 2.0323230824650658E-4 40-41 0.0 0.0 0.0 0.17569433047910493 2.0323230824650658E-4 42-43 0.0 0.0 0.0 0.2262229631168926 2.0323230824650658E-4 44-45 0.0 0.0 0.0 0.28952982713567943 2.0323230824650658E-4 46-47 0.0 0.0 0.0 0.37034007370219657 2.0323230824650658E-4 48-49 0.0 0.0 0.0 0.4544782493162503 2.0323230824650658E-4 50-51 0.0 0.0 0.0 0.54161410147694 2.0323230824650658E-4 52-53 0.0 0.0 0.0 0.6263111659386716 2.0323230824650658E-4 54-55 0.0 0.0 0.0 0.7243199465905494 2.540403853081332E-4 56-57 0.0 0.0 0.0 0.8367582211279292 2.540403853081332E-4 58-59 0.0 0.0 0.0 0.9704596759155997 2.540403853081332E-4 60-61 0.0 0.0 0.0 1.1078955243672999 2.540403853081332E-4 62-63 0.0 0.0 0.0 1.2535876853415142 3.0484846236975986E-4 64-65 0.0 0.0 0.0 1.413353683661799 3.0484846236975986E-4 66-67 2.5404038530813322E-5 0.0 0.0 1.5836877620109022 3.0484846236975986E-4 68-69 5.0808077061626644E-5 0.0 0.0 1.7767584548450837 3.0484846236975986E-4 70-71 5.0808077061626644E-5 0.0 0.0 1.9893140452323987 3.0484846236975986E-4 72-73 5.0808077061626644E-5 0.0 0.0 2.206035897938767 3.0484846236975986E-4 74-75 5.0808077061626644E-5 0.0 0.0 2.424002548533146 3.0484846236975986E-4 76-77 5.0808077061626644E-5 0.0 0.0 2.670650358628812 3.0484846236975986E-4 78-79 5.0808077061626644E-5 0.0 0.0 2.9415336214828742 3.0484846236975986E-4 80-81 5.0808077061626644E-5 0.0 0.0 3.238659256139267 3.0484846236975986E-4 82-83 1.0161615412325329E-4 0.0 0.0 3.552170495648034 3.0484846236975986E-4 84-85 1.0161615412325329E-4 0.0 0.0 3.8662914320815407 3.0484846236975986E-4 86-87 1.0161615412325329E-4 0.0 0.0 4.206019639354107 3.3025250090057314E-4 88-89 1.0161615412325329E-4 0.0 0.0 4.5753181474765405 3.556565394313865E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2575 0.0 60.518303 2 ACCTGGG 2810 0.0 55.96143 3 GTACCTG 2990 0.0 54.178005 1 CCTGGGG 2755 0.0 48.482452 4 CTGGGGG 2530 0.0 47.364944 5 GTACACG 325 0.0 36.4355 1 GTACCCG 250 0.0 34.10363 1 TGGGGGG 6565 0.0 31.38344 6 GTACTAG 1550 0.0 30.253218 1 CTGTGCG 2075 0.0 29.218636 9 TAGTACT 2115 0.0 28.889257 4 TATTCTC 1080 0.0 28.50668 5 GGGGGGA 6560 0.0 27.869518 7 GTACTGT 2255 0.0 27.515078 6 ACTGTGC 2380 0.0 27.46371 8 TATCACG 145 8.0891004E-7 26.133049 2 GGGGAAT 7015 0.0 25.928156 9 TAGGCAT 2055 0.0 25.814472 5 TCTAACG 940 0.0 25.698656 2 ATAACGC 320 0.0 25.16263 3 >>END_MODULE