##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931058_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1968191 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.64476415144669 33.0 33.0 33.0 33.0 33.0 2 32.594630805648436 33.0 33.0 33.0 33.0 33.0 3 32.645432785740816 33.0 33.0 33.0 33.0 33.0 4 36.592751414877924 37.0 37.0 37.0 37.0 37.0 5 36.54303164682696 37.0 37.0 37.0 37.0 37.0 6 36.54723499904227 37.0 37.0 37.0 37.0 37.0 7 36.48895102152179 37.0 37.0 37.0 37.0 37.0 8 36.49734553201392 37.0 37.0 37.0 37.0 37.0 9 36.483660376457365 37.0 37.0 37.0 37.0 37.0 10-11 36.46523863791674 37.0 37.0 37.0 37.0 37.0 12-13 36.3636067332896 37.0 37.0 37.0 37.0 37.0 14-15 38.293633087439176 40.0 37.0 40.0 37.0 40.0 16-17 38.28909389383449 40.0 37.0 40.0 37.0 40.0 18-19 38.260872039349834 40.0 37.0 40.0 37.0 40.0 20-21 38.1840857924866 40.0 37.0 40.0 35.0 40.0 22-23 38.04018969703652 40.0 37.0 40.0 33.0 40.0 24-25 38.196055921401936 40.0 37.0 40.0 37.0 40.0 26-27 38.13241575639763 40.0 37.0 40.0 35.0 40.0 28-29 38.04276592058393 40.0 37.0 40.0 33.0 40.0 30-31 37.900806629031436 40.0 37.0 40.0 33.0 40.0 32-33 37.76210261097627 40.0 37.0 40.0 33.0 40.0 34-35 37.66775734672092 40.0 37.0 40.0 33.0 40.0 36-37 37.595005007135995 40.0 37.0 40.0 33.0 40.0 38-39 37.42237364158255 40.0 37.0 40.0 33.0 40.0 40-41 37.210575091543454 40.0 37.0 40.0 33.0 40.0 42-43 37.00601923288949 40.0 37.0 40.0 33.0 40.0 44-45 36.72635785856149 40.0 37.0 40.0 33.0 40.0 46-47 36.41419201693331 37.0 37.0 40.0 33.0 40.0 48-49 36.09932369368623 37.0 37.0 40.0 33.0 40.0 50-51 35.807674915696694 37.0 37.0 40.0 33.0 40.0 52-53 35.54839139087619 37.0 37.0 40.0 27.0 40.0 54-55 35.288107200977954 37.0 37.0 40.0 27.0 40.0 56-57 34.9440387137224 37.0 33.0 40.0 27.0 40.0 58-59 34.647302014895914 37.0 33.0 40.0 27.0 40.0 60-61 34.3934463169479 37.0 33.0 40.0 27.0 40.0 62-63 34.05952090015654 37.0 33.0 38.5 27.0 40.0 64-65 33.69433886243763 37.0 33.0 37.0 27.0 40.0 66-67 33.33383726477766 37.0 33.0 37.0 24.5 40.0 68-69 33.017363152255044 37.0 33.0 37.0 22.0 40.0 70-71 32.746415617183494 37.0 33.0 37.0 22.0 40.0 72-73 32.42095889067677 37.0 33.0 37.0 22.0 40.0 74-75 32.02836741962543 37.0 33.0 37.0 22.0 37.0 76-77 30.367469417348214 33.0 30.0 37.0 18.5 37.0 78-79 31.172225154977337 33.0 33.0 37.0 15.0 37.0 80-81 31.250604743137224 33.0 33.0 37.0 15.0 37.0 82-83 31.026455257645218 33.0 33.0 37.0 15.0 37.0 84-85 30.735908506847153 33.0 33.0 37.0 15.0 37.0 86-87 30.394302433046384 33.0 33.0 37.0 15.0 37.0 88-89 30.062051650474977 33.0 30.0 37.0 6.0 37.0 90-91 29.641278717360258 33.0 27.0 37.0 4.0 37.0 92-93 29.22160019022544 33.0 27.0 37.0 2.0 37.0 94-95 28.727106515576992 33.0 27.0 37.0 2.0 37.0 96-97 28.21638601131699 33.0 27.0 37.0 2.0 37.0 98-99 27.5364291372128 33.0 27.0 37.0 2.0 37.0 100-101 25.822445331779285 33.0 21.0 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.040885540193571046 1101 2 0.18196128159416958 1101 3 0.07269937358633882 1101 4 0.15243835872521316 1101 5 0.03853826000177207 1101 6 0.023319100959270145 1101 7 0.15148748237532317 1101 8 0.057502868240121074 1101 9 0.1533374677093775 1101 10-11 0.10535152031017958 1101 12-13 0.1450851971928273 1101 14-15 0.2011117320832767 1101 16-17 0.17034448399681423 1101 18-19 0.12210513653739241 1101 20-21 0.05608995106546644 1101 22-23 0.10295702426965647 1101 24-25 0.11942268666659572 1101 26-27 0.08317821170091833 1101 28-29 0.11788217156322389 1101 30-31 0.15481034101704694 1101 32-33 0.16537133228283807 1101 34-35 0.21224883132304484 1101 36-37 0.08344177985376433 1101 38-39 0.06661790089525255 1101 40-41 0.013448429895326797 1101 42-43 -0.05287939434150246 1101 44-45 -0.04813860925954572 1101 46-47 -0.1269597200456971 1101 48-49 -0.14532101532105202 1101 50-51 -0.19408248502590908 1101 52-53 -0.36874637635987284 1101 54-55 -0.4597173317260115 1101 56-57 -0.4460787508624833 1101 58-59 -0.49231709835067505 1101 60-61 -0.46047960838160407 1101 62-63 -0.5238035933850469 1101 64-65 -0.5366166459924315 1101 66-67 -0.5683995086131972 1101 68-69 -0.6883275301101648 1101 70-71 -0.6335666376430922 1101 72-73 -0.7959839537344209 1101 74-75 -0.9684497200504047 1101 76-77 -0.939419413922522 1101 78-79 -0.9775518959975784 1101 80-81 -1.2487625127767465 1101 82-83 -1.4754006082600277 1101 84-85 -1.4782209142001435 1101 86-87 -1.7217154309527736 1101 88-89 -1.7611146856370326 1101 90-91 -1.8270610541546866 1101 92-93 -1.7245695679335 1101 94-95 -2.1186242765294914 1101 96-97 -2.267972560690499 1101 98-99 -2.51429567183591 1101 100-101 -3.895082899862423 1102 1 0.07510149334048322 1102 2 0.1923810812121829 1102 3 0.13789807408570454 1102 4 0.1217750930953585 1102 5 0.16037596201764615 1102 6 0.13593847037150653 1102 7 0.17787438838361425 1102 8 0.1820750419214292 1102 9 0.18082495176702196 1102 10-11 0.15466433320873563 1102 12-13 0.1695177760797506 1102 14-15 0.2410447265819755 1102 16-17 0.22067173861137235 1102 18-19 0.21052955547406071 1102 20-21 0.054757138040010034 1102 22-23 0.10999926438539376 1102 24-25 0.18865186959288138 1102 26-27 0.17632712127521444 1102 28-29 0.30001372062127984 1102 30-31 0.25440361254796073 1102 32-33 0.28584528833585665 1102 34-35 0.27862790098718193 1102 36-37 0.321360643552147 1102 38-39 0.36316213658202656 1102 40-41 0.26989897843942146 1102 42-43 0.2972365645161048 1102 44-45 0.3635321500726789 1102 46-47 0.37418844713810273 1102 48-49 0.3749341893439393 1102 50-51 0.3469461699782457 1102 52-53 0.358607699490868 1102 54-55 0.26772430813210946 1102 56-57 0.19864313823580204 1102 58-59 0.26370260989620675 1102 60-61 0.08538537172614014 1102 62-63 0.055770991479064946 1102 64-65 0.07138721190006692 1102 66-67 -0.020215509983756874 1102 68-69 -0.019003168914977664 1102 70-71 -0.026723320109347526 1102 72-73 -0.19375107154608173 1102 74-75 -0.18714828412758422 1102 76-77 -0.2788435219435108 1102 78-79 -0.1695305887557801 1102 80-81 -0.27819068173721107 1102 82-83 -0.4657022599202527 1102 84-85 -0.4712943156811171 1102 86-87 -0.6091311188695769 1102 88-89 -0.8213036779529723 1102 90-91 -0.8198793778750115 1102 92-93 -0.8811470208361207 1102 94-95 -0.8579658967174382 1102 96-97 -0.8391904935454058 1102 98-99 -1.0037091516168708 1102 100-101 -1.8250968910679504 1103 1 0.09675844129424149 1103 2 0.20523442124474656 1103 3 0.14324618437176184 1103 4 0.09416976141105948 1103 5 0.10606148153800632 1103 6 0.14667937930870067 1103 7 0.0773219435677035 1103 8 0.1369446243630037 1103 9 0.0845674211736096 1103 10-11 0.11432402526966712 1103 12-13 0.15303320443584312 1103 14-15 0.19454527492028717 1103 16-17 0.085977382872656 1103 18-19 0.050986053162354494 1103 20-21 0.107892208019571 1103 22-23 0.08603543055847496 1103 24-25 0.04861121525858891 1103 26-27 0.03926247049667353 1103 28-29 0.07940054794060813 1103 30-31 0.0800839627114911 1103 32-33 0.15080912699394844 1103 34-35 0.05723449360805688 1103 36-37 0.21375106043419834 1103 38-39 0.2597864741072087 1103 40-41 0.06554331219629006 1103 42-43 -0.012293772844820694 1103 44-45 0.242760031747558 1103 46-47 0.2074144881354485 1103 48-49 0.3717570881453369 1103 50-51 0.1600678817600354 1103 52-53 -0.033371463653068645 1103 54-55 0.11927559829728551 1103 56-57 0.11271345115097375 1103 58-59 0.05060674489855188 1103 60-61 0.11855380096633894 1103 62-63 0.050075036621564095 1103 64-65 0.03469927196123024 1103 66-67 0.1625991564960998 1103 68-69 -0.04049827251951399 1103 70-71 -0.07409540268378834 1103 72-73 -0.0925911065237841 1103 74-75 -0.09870940573308218 1103 76-77 -0.2986253702542925 1103 78-79 -0.05134616832764394 1103 80-81 -0.2481794251003855 1103 82-83 -0.3250087931176715 1103 84-85 -0.4229040630589971 1103 86-87 -0.3675023366637582 1103 88-89 -0.2983990791578677 1103 90-91 -0.10002349181343817 1103 92-93 -0.21491354949223407 1103 94-95 -0.3429222228990234 1103 96-97 -0.0776355316875268 1103 98-99 -0.16507219506362603 1103 100-101 -1.0285038353983254 1104 1 0.08450651380420027 1104 2 0.1814669044710442 1104 3 0.07861834641327192 1104 4 0.16606171878083842 1104 5 0.09098701772457218 1104 6 0.12123729598190636 1104 7 0.07797584330008789 1104 8 0.15440756069807549 1104 9 0.16043493189060598 1104 10-11 0.12836436291971154 1104 12-13 0.10102994951813571 1104 14-15 0.12266088843077938 1104 16-17 0.12658842581469543 1104 18-19 0.16673696110717628 1104 20-21 0.09838851006516336 1104 22-23 0.10267286054185831 1104 24-25 0.15873303352606172 1104 26-27 0.12703252659969877 1104 28-29 0.04105263739140241 1104 30-31 0.019253341809580604 1104 32-33 -0.017486774411878514 1104 34-35 0.10542875454432732 1104 36-37 0.1793015924147241 1104 38-39 0.09526732034095176 1104 40-41 0.03640411567418056 1104 42-43 -0.013473147748079839 1104 44-45 0.11145180607027072 1104 46-47 -0.06523225795628917 1104 48-49 -0.06594269897272653 1104 50-51 -0.10773854821660933 1104 52-53 -0.05841144911698137 1104 54-55 -0.12854034294819172 1104 56-57 -0.1951294760766018 1104 58-59 -0.07884016584581843 1104 60-61 -0.03775711657948477 1104 62-63 -0.1766215135245801 1104 64-65 0.012182913251798766 1104 66-67 0.15300292556069905 1104 68-69 -0.2416408585527563 1104 70-71 -0.12232471399165235 1104 72-73 -0.011848509723066059 1104 74-75 -0.16049563996602245 1104 76-77 -0.2941930404645028 1104 78-79 -0.14342293368867942 1104 80-81 -0.16102176908978194 1104 82-83 -0.0438375162522604 1104 84-85 -0.1158569194679977 1104 86-87 -0.2753694907081794 1104 88-89 -0.16428261770878905 1104 90-91 -0.19723667882875873 1104 92-93 -0.14922522749091627 1104 94-95 -0.012985784644879317 1104 96-97 -0.04762708594998699 1104 98-99 -0.050712085863491296 1104 100-101 -1.0960958131814387 1105 1 0.13098722878392266 1105 2 0.2030611258678121 1105 3 0.1040599339141508 1105 4 0.08074969660833631 1105 5 0.04577200023299355 1105 6 0.0831926540318122 1105 7 0.10953753009791711 1105 8 0.07184548056762452 1105 9 0.06698899949693526 1105 10-11 0.06239688188954062 1105 12-13 0.1450988322190554 1105 14-15 0.08268722348244495 1105 16-17 0.146908744440168 1105 18-19 0.15494115022983834 1105 20-21 0.0837970419967391 1105 22-23 0.17130510496031093 1105 24-25 0.22428464996684738 1105 26-27 0.2740420264942003 1105 28-29 0.30948873646953956 1105 30-31 0.2706485004320385 1105 32-33 0.40632951581029175 1105 34-35 0.34809531528035365 1105 36-37 0.36093178681480964 1105 38-39 0.3406855162726927 1105 40-41 0.300695374573003 1105 42-43 0.49212767434314486 1105 44-45 0.5630177127280049 1105 46-47 0.45693516107840537 1105 48-49 0.5857783266095211 1105 50-51 0.5275814187538685 1105 52-53 0.4271057051345295 1105 54-55 0.3509553056468846 1105 56-57 0.47299378030073314 1105 58-59 0.5057122815426851 1105 60-61 0.3834093881522307 1105 62-63 0.3594325006822814 1105 64-65 0.39134592227240006 1105 66-67 0.5957934772422604 1105 68-69 0.42551417441164574 1105 70-71 0.5098853204184408 1105 72-73 0.409648029731315 1105 74-75 0.44419999612576433 1105 76-77 0.3527497752239981 1105 78-79 0.5667798800722323 1105 80-81 0.544808931172529 1105 82-83 0.6289023189648155 1105 84-85 0.6294800050876788 1105 86-87 0.7026604077807441 1105 88-89 0.6746306120730026 1105 90-91 0.5931589721106789 1105 92-93 0.6641198189926634 1105 94-95 0.375382770626171 1105 96-97 0.39454259930576896 1105 98-99 0.4211848193120886 1105 100-101 -0.4026847858018421 1106 1 0.0974947517295135 1106 2 0.15801382187106583 1106 3 0.12203074328152752 1106 4 0.15458338866205423 1106 5 0.09154353332033338 1106 6 0.07502324806512917 1106 7 0.17908897900158394 1106 8 0.07916795852572989 1106 9 0.07627618751520515 1106 10-11 0.12189401997546412 1106 12-13 0.1532295447510208 1106 14-15 0.1634504769384506 1106 16-17 0.16319448018182925 1106 18-19 0.18857745485407662 1106 20-21 0.08966571597018458 1106 22-23 0.17093446258564882 1106 24-25 0.20965891348694754 1106 26-27 0.18257774728034093 1106 28-29 0.2603013622391117 1106 30-31 0.26466221878201424 1106 32-33 0.10092210830860182 1106 34-35 0.160474286925961 1106 36-37 0.20266571090184726 1106 38-39 0.2449961430936085 1106 40-41 0.20675967949762963 1106 42-43 0.2483573809730828 1106 44-45 0.3405745023439124 1106 46-47 0.3289666809539753 1106 48-49 0.2031026755988492 1106 50-51 0.24942793564469667 1106 52-53 0.24662297908022168 1106 54-55 0.2589097339450106 1106 56-57 0.2306758908239388 1106 58-59 0.2860663264703476 1106 60-61 0.34533097539765834 1106 62-63 0.25073360560894287 1106 64-65 0.36904997604609235 1106 66-67 0.3933527361102449 1106 68-69 0.33405218625641453 1106 70-71 0.5770348489058463 1106 72-73 0.5381487991451763 1106 74-75 0.44070855004773435 1106 76-77 0.3467046897356916 1106 78-79 0.6191365520804588 1106 80-81 0.509953146949524 1106 82-83 0.5743989250160091 1106 84-85 0.7180730787083043 1106 86-87 0.6342800716824435 1106 88-89 0.5740475376505039 1106 90-91 0.5158100324253461 1106 92-93 0.6268306759376863 1106 94-95 0.8636950622830248 1106 96-97 0.9750701282844787 1106 98-99 1.0837952515202964 1106 100-101 0.882646074885244 1107 1 0.05908411944632519 1107 2 0.15437976382231255 1107 3 0.007581096169374746 1107 4 0.03979637785482737 1107 5 0.01102014610005142 1107 6 0.007603283779324954 1107 7 0.02517863044421631 1107 8 0.025852657380539767 1107 9 0.015721870357324974 1107 10-11 0.02549518013172758 1107 12-13 0.11265995744694379 1107 14-15 0.08812478410364832 1107 16-17 -0.008100702940325277 1107 18-19 0.1493904914947919 1107 20-21 0.17178282605556205 1107 22-23 0.054658291929932545 1107 24-25 0.06578760096708436 1107 26-27 0.11367922741423087 1107 28-29 0.1581853421738657 1107 30-31 0.15556785041291477 1107 32-33 0.1312099944611731 1107 34-35 0.08718177755905998 1107 36-37 0.14117809483752097 1107 38-39 0.061088416009987156 1107 40-41 0.22037321705438018 1107 42-43 0.1303227513789409 1107 44-45 -0.06999292218267783 1107 46-47 -0.04697114942754865 1107 48-49 0.16550561263587582 1107 50-51 0.08897783624818345 1107 52-53 0.0031769716981742135 1107 54-55 0.08229774337837625 1107 56-57 0.04148366232218592 1107 58-59 -0.0012441129877913681 1107 60-61 -0.011672810819312929 1107 62-63 0.019052417260127186 1107 64-65 0.12852791066017488 1107 66-67 0.07857650219883539 1107 68-69 0.15483006604890193 1107 70-71 0.14979985758284187 1107 72-73 0.12622575456606455 1107 74-75 0.19059763680076358 1107 76-77 0.18873857851072628 1107 78-79 0.4026114523941722 1107 80-81 0.21771394227950225 1107 82-83 0.25759776910145504 1107 84-85 0.23100560029723383 1107 86-87 0.3044807956826894 1107 88-89 0.27805096948762653 1107 90-91 0.3389461288363904 1107 92-93 0.5329903007537453 1107 94-95 0.48050058321030065 1107 96-97 0.3215562067299196 1107 98-99 0.6528761401382432 1107 100-101 1.4523773997208593 1108 1 0.015572457326534561 1108 2 0.037182745448724575 1108 3 0.05414601114808448 1108 4 0.04016715005112559 1108 5 0.04160042565386135 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0.13626005270373298 2316 86-87 0.042995840806955954 2316 88-89 -0.006057391813218516 2316 90-91 0.14604410793726075 2316 92-93 0.2182169325622496 2316 94-95 0.3011078921013528 2316 96-97 0.5652791932660968 2316 98-99 0.8563495582806873 2316 100-101 1.4787112941960885 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 14.0 5 41.0 6 50.0 7 167.0 8 429.0 9 876.0 10 1575.0 11 2524.0 12 3882.0 13 5701.0 14 6992.0 15 8633.0 16 11159.0 17 14617.0 18 15302.0 19 11289.0 20 10000.0 21 9685.0 22 10254.0 23 11525.0 24 13459.0 25 15846.0 26 18994.0 27 23343.0 28 28504.0 29 35095.0 30 44564.0 31 57139.0 32 74677.0 33 100532.0 34 143994.0 35 220310.0 36 350507.0 37 498651.0 38 217829.0 39 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.69895725227559 22.5442393616291 13.469837503832697 24.28696588226261 2 14.08083382363252 21.38278597652864 40.81824583652155 23.71813436331729 3 17.1536705533152 28.726378689873087 29.97300566865716 24.146945088154553 4 11.823852461473505 17.779372022329134 39.13207610440247 31.264699411794894 5 12.350732220602573 38.382148886972864 35.05818286944712 14.208936022977445 6 28.520504361619377 38.69228138935703 19.19351323118539 13.593701017838209 7 26.03476999945635 32.214200755922576 24.287886693923507 17.46314255069757 8 26.19141130103735 34.7912880406424 20.530527779062094 18.48677287925816 9 26.646702479586587 14.993260308577774 22.19286644436439 36.16717076747125 10-11 25.41841721662176 26.292773414775294 27.947948141211903 20.34086122739104 12-13 25.540839278301753 24.441835167420233 28.27339927883015 21.743926275447862 14-15 22.8031801872384 25.43082818392164 26.413966972633684 25.352024656206275 16-17 21.298999944619197 27.40491141357724 27.916243901125448 23.379844740678116 18-19 20.95053274809203 27.790925779052948 30.01212788799461 21.246413584860413 20-21 22.08870988638806 27.10072345620928 29.0471808884402 21.76338576896246 22-23 22.44822783967613 26.9455809929016 28.408015279004932 22.198175888417335 24-25 21.918731464578386 27.072880629979508 29.445084343948324 21.563303561493775 26-27 21.998601761719264 26.992603868222137 28.765907373827034 22.242886996231565 28-29 21.30352186347768 27.46908201490607 29.18664905997436 22.040747061641884 30-31 22.221827048289523 27.14383410959607 28.892749738211382 21.741589103903024 32-33 22.649789235340787 27.512301273682603 28.059860059277796 21.77804943169881 34-35 22.20155462554193 27.099123001781837 29.14978780006615 21.549534572610078 36-37 22.376258198518332 26.76236706701738 28.77820292847594 22.083171805988343 38-39 22.431483968257112 26.76833881299001 29.29314715202656 21.507030066726315 40-41 21.95186950445563 25.97388839194189 29.761395152806337 22.31284695079615 42-43 22.220726123384626 26.41940895232235 28.785825180512088 22.574039743780936 44-45 21.849200611119553 27.647291345199733 28.694877682094877 21.80863036158584 46-47 22.156665689457984 27.099910526976295 28.18989620417937 22.55352757938635 48-49 22.924283655829626 26.66323034181694 28.262772661961154 22.149713340392278 50-51 22.343741029198895 27.018287351176788 27.63847614382954 22.999495475794777 52-53 21.852452328051495 27.544862261843488 27.538104787594293 23.06458062251072 54-55 22.08052978598114 27.506171911161058 27.499185800565083 22.914112502292713 56-57 23.039430624365217 26.829408324700193 27.410398685899896 22.720762365034695 58-59 22.047656960122264 27.248371728150367 28.172113377207804 22.531857934519568 60-61 22.378696986217292 28.03998697280904 27.213110922669596 22.368205118304065 62-63 22.666753379118184 27.1670279967747 26.83372701125043 23.33249161285668 64-65 21.560153460715956 27.49209807381499 27.430772724801606 23.516975740667444 66-67 22.421160454296256 26.67204567960763 27.362696714618806 23.544097151477306 68-69 22.003715345209024 26.947558349516832 27.380981416160328 23.667744889113816 70-71 21.9156001283428 26.731529065249305 27.746331420639464 23.60653938576843 72-73 22.635430199609694 26.732847574244573 27.8159487570058 22.815773469139934 74-75 22.669819140890652 27.478045178068474 27.194027810838335 22.65810787020254 76-77 22.450394990620623 27.07273538372114 27.15281066261277 23.324058963045463 78-79 21.86852716061521 27.28789665254884 27.479098278296178 23.364477908539772 80-81 21.488634414953413 27.564684437500397 28.080915089965643 22.86576605758055 82-83 22.422365203270374 26.421312407492938 28.864565851308853 22.29175653792783 84-85 22.746198009142105 27.113377992949207 27.281404102794177 22.859019895114514 86-87 21.58916342531564 27.71663152856703 27.825538890750444 22.86866615536688 88-89 22.271898408233753 26.708840757832952 27.637434578249774 23.381826255683517 90-91 22.00345393307865 26.53474688178129 28.088762726788204 23.373036458351855 92-93 22.38717775643255 26.86803272170873 27.630871973431205 23.113917548427516 94-95 20.926847795297295 27.042346195422283 28.327301781266158 23.703504228014264 96-97 21.35583411537498 27.26626523838001 27.510048832933037 23.867851813311965 98-99 20.779588972818186 27.96446076625693 27.330680812990206 23.925269447934678 100-101 19.626584899285383 28.305570868592127 27.247184588847084 24.820659643275402 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 87.0 1 69.5 2 79.5 3 98.5 4 74.0 5 83.0 6 146.0 7 252.5 8 393.0 9 510.0 10 557.5 11 604.0 12 738.0 13 898.5 14 1090.0 15 1351.0 16 1580.0 17 1809.5 18 2063.0 19 2312.5 20 2599.5 21 2836.5 22 3127.5 23 3549.0 24 4053.0 25 4785.0 26 5946.5 27 7352.5 28 9739.0 29 14892.0 30 21801.0 31 27519.5 32 35322.0 33 42767.0 34 47928.5 35 54192.0 36 58030.5 37 66009.0 38 68813.0 39 62439.0 40 57571.5 41 52997.0 42 51538.5 43 54532.5 44 61583.5 45 72773.0 46 80609.0 47 85515.0 48 91262.5 49 88127.5 50 82790.0 51 81862.0 52 78445.5 53 74105.0 54 70555.5 55 63011.0 56 54171.5 57 43976.0 58 35080.5 59 32973.0 60 27720.0 61 19430.0 62 13974.0 63 11070.0 64 8198.5 65 4609.5 66 2785.0 67 1906.0 68 1417.0 69 1081.0 70 859.0 71 672.0 72 511.5 73 421.5 74 342.5 75 271.0 76 228.5 77 177.5 78 132.0 79 109.0 80 79.0 81 53.0 82 40.0 83 33.0 84 28.5 85 30.0 86 27.5 87 15.0 88 9.0 89 8.5 90 6.5 91 4.0 92 3.0 93 1.0 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07926060021613757 2 2.540403853081332E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.540403853081332E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 2.5404038530813322E-5 34-35 0.0 36-37 0.0 38-39 1.0161615412325329E-4 40-41 0.003175504816351665 42-43 0.005563484438248117 44-45 0.0 46-47 0.0 48-49 1.5242423118487993E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.002438787698958079 68-69 0.006960706557442849 70-71 0.0012447978880098527 72-73 0.0 74-75 7.621211559243997E-5 76-77 0.0021847473136499453 78-79 0.005665100592371371 80-81 0.003988434049337692 82-83 0.004979191552039411 84-85 0.0022609594292423854 86-87 2.2863634677731988E-4 88-89 0.0 90-91 0.0 92-93 8.891413485784662E-4 94-95 0.0034803532787214247 96-97 0.002642020007204585 98-99 0.0 100-101 3.0484846236975986E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1968191.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.2314467387235 #Duplication Level Percentage of deduplicated Percentage of total 1 83.55883936694036 29.43898798705911 2 9.679543419074731 6.820486368485859 3 2.4103980200110646 2.547654283834332 4 0.9847442692978849 1.3877586110012647 5 0.5727539197334398 1.0089474608741897 6 0.35286334000589226 0.7459131581679405 7 0.2638018662174503 0.6505884979451171 8 0.1960020033725285 0.5524347314001872 9 0.15053950309503852 0.47733520368300764 >10 1.2579420199594145 9.298211384883777 >50 0.23642221067533414 5.955689744575944 >100 0.2834406037332596 21.79914128024639 >500 0.03659168977048901 8.645525518459229 >1k 0.015391742522507284 9.151425634153846 >5k 7.260255906843059E-4 1.5199001352297594 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 6344 0.3223264408789594 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 6327 0.32146270356891177 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 6308 0.3204973501047409 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5386 0.2736523030539211 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 5345 0.2715691718943944 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 4830 0.24540301220765665 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 4480 0.22762018523608735 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 4354 0.22121836752632237 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4300 0.21847473136499457 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 3329 0.1691400885381551 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3014 0.1531355442637427 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 2882 0.14642887809160798 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 2510 0.12752827342468287 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 2489 0.1264613038063887 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 2456 0.12478463726335502 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 2445 0.12422574841567714 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 2421 0.12300635456619811 No Hit GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 2416 0.12275231418088996 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 2398 0.12183776879378068 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 2308 0.11726504185823429 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 2288 0.11624888031700176 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 2223 0.11294635530799602 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 2220 0.11279393107681114 No Hit GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG 2183 0.11091403222553096 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 2134 0.10842443644951125 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 2092 0.10629049721292293 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 2060 0.10466463874695088 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 2056 0.10446140643870439 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 2053 0.1043089822075195 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 2036 0.10344524489747184 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 2010 0.10212423489386956 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 1982 0.100701608736144 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 1970 0.10009191181140449 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 5.0808077061626644E-5 14-15 0.0 0.0 0.0 0.0 5.0808077061626644E-5 16-17 0.0 0.0 0.0 0.0 1.0161615412325329E-4 18-19 0.0 0.0 0.0 0.0 1.0161615412325329E-4 20-21 0.0 0.0 0.0 1.0161615412325329E-4 1.0161615412325329E-4 22-23 0.0 0.0 0.0 1.5242423118487993E-4 1.0161615412325329E-4 24-25 0.0 0.0 0.0 2.540403853081332E-4 1.0161615412325329E-4 26-27 0.0 0.0 0.0 6.351009632703331E-4 1.0161615412325329E-4 28-29 0.0 0.0 0.0 0.001905302889810999 1.0161615412325329E-4 30-31 0.0 0.0 0.0 0.006681262133603903 1.0161615412325329E-4 32-33 0.0 0.0 0.0 0.02011999851640415 1.0161615412325329E-4 34-35 0.0 0.0 0.0 0.04270418877029719 1.0161615412325329E-4 36-37 0.0 0.0 0.0 0.07824443867490502 1.0161615412325329E-4 38-39 0.0 0.0 0.0 0.1256737786119335 1.2702019265406662E-4 40-41 0.0 0.0 0.0 0.17541488605526598 1.5242423118487993E-4 42-43 0.0 0.0 0.0 0.22553705407656066 1.5242423118487993E-4 44-45 0.0 0.0 0.0 0.2886152817485701 1.5242423118487993E-4 46-47 0.0 0.0 0.0 0.3688158313903478 1.5242423118487993E-4 48-49 0.0 0.0 0.0 0.45209026969435384 1.7782826971569324E-4 50-51 0.0 0.0 0.0 0.5379813239670337 2.0323230824650658E-4 52-53 0.0 0.0 0.0 0.621077934001324 2.0323230824650658E-4 54-55 0.0 0.0 0.0 0.7175116642642914 2.0323230824650658E-4 56-57 0.0 2.5404038530813322E-5 0.0 0.8285527166824764 2.0323230824650658E-4 58-59 0.0 5.0808077061626644E-5 0.0 0.9601202322335587 2.0323230824650658E-4 60-61 0.0 5.0808077061626644E-5 0.0 1.0951426970248315 2.0323230824650658E-4 62-63 0.0 5.0808077061626644E-5 0.0 1.2379133935680022 2.0323230824650658E-4 64-65 0.0 5.0808077061626644E-5 0.0 1.3950373718810827 2.0323230824650658E-4 66-67 0.0 5.0808077061626644E-5 0.0 1.5636439756100908 2.0323230824650658E-4 68-69 0.0 5.0808077061626644E-5 0.0 1.7543520928609062 2.0323230824650658E-4 70-71 0.0 5.0808077061626644E-5 0.0 1.9634273299694998 2.0323230824650658E-4 72-73 0.0 5.0808077061626644E-5 0.0 2.17644019305037 2.0323230824650658E-4 74-75 0.0 5.0808077061626644E-5 0.0 2.3902913894027558 2.0323230824650658E-4 76-77 0.0 5.0808077061626644E-5 0.0 2.6321632402546298 2.0323230824650658E-4 78-79 0.0 5.0808077061626644E-5 0.0 2.8979656954025295 2.0323230824650658E-4 80-81 0.0 5.0808077061626644E-5 0.0 3.1905694112004372 2.0323230824650658E-4 82-83 0.0 5.0808077061626644E-5 0.0 3.4985171662709567 2.0323230824650658E-4 84-85 0.0 5.0808077061626644E-5 0.0 3.804458002297541 2.0323230824650658E-4 86-87 0.0 5.0808077061626644E-5 0.0 4.136285553587024 2.0323230824650658E-4 88-89 0.0 5.0808077061626644E-5 0.0 4.495422446297133 2.0323230824650658E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2735 0.0 59.39426 2 ACCTGGG 2995 0.0 53.920982 3 CTGGGGG 2380 0.0 53.48519 5 GTACCTG 3100 0.0 53.05168 1 CCTGGGG 2675 0.0 52.73615 4 GTACACG 245 0.0 46.561817 1 TACGTGG 135 7.2759576E-12 38.702106 2 GTACCCG 260 0.0 38.391113 1 TGGGGGG 5915 0.0 35.091537 6 GTACTAG 1550 0.0 33.425625 1 GTACGTG 175 3.6379788E-12 32.593273 1 TATCACG 120 1.5072874E-7 31.665363 2 TAACGCA 240 0.0 31.665363 4 GGGGGGA 6150 0.0 30.275175 7 CAGTACG 280 0.0 28.838097 9 CTGTGCG 2390 0.0 27.823124 9 AGGGCTA 2705 0.0 27.216995 5 ATAACGC 265 0.0 26.885685 3 CTATTGA 2665 0.0 26.377901 9 GCTATTG 2705 0.0 25.98784 8 >>END_MODULE