##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931057_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3621516 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.678652531149936 33.0 33.0 33.0 33.0 33.0 2 32.63861681130223 33.0 33.0 33.0 33.0 33.0 3 32.68691095110445 33.0 33.0 33.0 33.0 33.0 4 36.63531432692828 37.0 37.0 37.0 37.0 37.0 5 36.59857087473865 37.0 37.0 37.0 37.0 37.0 6 36.60544120197177 37.0 37.0 37.0 37.0 37.0 7 36.552886415523226 37.0 37.0 37.0 37.0 37.0 8 36.56740243588597 37.0 37.0 37.0 37.0 37.0 9 36.557370449281464 37.0 37.0 37.0 37.0 37.0 10-11 36.536597518829126 37.0 37.0 37.0 37.0 37.0 12-13 36.47969123980123 37.0 37.0 37.0 37.0 37.0 14-15 38.540815365719766 40.0 37.0 40.0 37.0 40.0 16-17 38.54895864052513 40.0 37.0 40.0 37.0 40.0 18-19 38.51803388415239 40.0 37.0 40.0 37.0 40.0 20-21 38.456491149010525 40.0 37.0 40.0 37.0 40.0 22-23 38.365954202604655 40.0 37.0 40.0 37.0 40.0 24-25 38.48599067351904 40.0 37.0 40.0 37.0 40.0 26-27 38.42212722517311 40.0 37.0 40.0 37.0 40.0 28-29 38.37755818281626 40.0 37.0 40.0 37.0 40.0 30-31 38.27615023653078 40.0 37.0 40.0 37.0 40.0 32-33 38.175655029551166 40.0 37.0 40.0 37.0 40.0 34-35 38.122815693759186 40.0 37.0 40.0 37.0 40.0 36-37 38.09040550973681 40.0 37.0 40.0 37.0 40.0 38-39 37.97486163253179 40.0 37.0 40.0 33.0 40.0 40-41 37.82800517794205 40.0 37.0 40.0 33.0 40.0 42-43 37.711403594516774 40.0 37.0 40.0 33.0 40.0 44-45 37.53713403447617 40.0 37.0 40.0 33.0 40.0 46-47 37.36433968536933 40.0 37.0 40.0 33.0 40.0 48-49 37.20800985001861 40.0 37.0 40.0 33.0 40.0 50-51 37.04469978870727 40.0 37.0 40.0 33.0 40.0 52-53 36.859903007469796 40.0 37.0 40.0 33.0 40.0 54-55 36.69326533418601 37.0 37.0 40.0 33.0 40.0 56-57 36.45495091006087 37.0 37.0 40.0 33.0 40.0 58-59 36.24332544713319 37.0 37.0 40.0 33.0 40.0 60-61 36.05353117313302 37.0 37.0 40.0 33.0 40.0 62-63 35.806873419860636 37.0 37.0 40.0 33.0 40.0 64-65 35.50949381419273 37.0 35.0 40.0 27.0 40.0 66-67 35.23073279256532 37.0 33.0 40.0 27.0 40.0 68-69 35.01840638009055 37.0 33.0 40.0 27.0 40.0 70-71 34.79252956496671 37.0 33.0 40.0 27.0 40.0 72-73 34.46484524712855 37.0 33.0 37.0 27.0 40.0 74-75 34.07780470940899 37.0 33.0 37.0 27.0 40.0 76-77 32.437345713783955 33.0 33.0 37.0 27.0 37.0 78-79 33.1900744053043 37.0 33.0 37.0 27.0 37.0 80-81 33.21661867571481 37.0 33.0 37.0 27.0 37.0 82-83 33.000348472849495 37.0 33.0 37.0 27.0 37.0 84-85 32.70479006581774 37.0 33.0 37.0 27.0 37.0 86-87 32.37180755241728 35.0 33.0 37.0 27.0 37.0 88-89 32.05009518113409 33.0 33.0 37.0 24.5 37.0 90-91 31.6866868736739 33.0 33.0 37.0 22.0 37.0 92-93 31.302579223728408 33.0 33.0 37.0 22.0 37.0 94-95 30.922665673712334 33.0 33.0 37.0 18.5 37.0 96-97 30.49945105861744 33.0 33.0 37.0 15.0 37.0 98-99 29.955071991950334 33.0 33.0 37.0 4.0 37.0 100-101 28.26335821793967 33.0 30.0 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06949604978909463 1101 2 0.14156120668013017 1101 3 0.062183403420796424 1101 4 0.1166596402579998 1101 5 0.04902595463635606 1101 6 0.05312218919490164 1101 7 0.050252811962053556 1101 8 0.09831257392790604 1101 9 0.1300709100678361 1101 10-11 0.09078121855046106 1101 12-13 0.17877580416823946 1101 14-15 0.2401180275653303 1101 16-17 0.2508653233959919 1101 18-19 0.2024725134742269 1101 20-21 0.12544664145961093 1101 22-23 0.09646150350269522 1101 24-25 0.20990731536221574 1101 26-27 0.1566060315987201 1101 28-29 0.2194963515300543 1101 30-31 0.14959892477777714 1101 32-33 0.17891037294617718 1101 34-35 0.1704286136660187 1101 36-37 0.11800301384399603 1101 38-39 0.15128603976059196 1101 40-41 3.84486456695754E-4 1101 42-43 0.006190869915023711 1101 44-45 0.019657243161184113 1101 46-47 -0.052656755926534515 1101 48-49 -0.07198602889830852 1101 50-51 -0.06298087061730939 1101 52-53 -0.2746876029956411 1101 54-55 -0.2751213785392608 1101 56-57 -0.3399550510618141 1101 58-59 -0.356059860420352 1101 60-61 -0.4365478429564291 1101 62-63 -0.3975078370813705 1101 64-65 -0.359756967774544 1101 66-67 -0.4862388092742478 1101 68-69 -0.7497212027249489 1101 70-71 -0.6059366428705388 1101 72-73 -0.7136699149568742 1101 74-75 -0.8544107714497287 1101 76-77 -0.8483569466688365 1101 78-79 -0.673070115336067 1101 80-81 -0.8926293045848936 1101 82-83 -0.9264802244619403 1101 84-85 -0.9444493557440552 1101 86-87 -1.0625774125087446 1101 88-89 -1.1928814814083175 1101 90-91 -1.3353265966134238 1101 92-93 -1.294718669245281 1101 94-95 -1.297827313389945 1101 96-97 -1.5386659798726576 1101 98-99 -1.5508126039695327 1101 100-101 -2.9700015119654744 1102 1 0.10538657123136375 1102 2 0.16768044267072213 1102 3 0.13825649963094122 1102 4 0.1192507849720954 1102 5 0.13283033522785814 1102 6 0.1386643002396326 1102 7 0.08740439268464684 1102 8 0.14286700241147798 1102 9 0.11320840093045348 1102 10-11 0.11706502804585739 1102 12-13 0.08636061813032825 1102 14-15 0.19688539664118565 1102 16-17 0.1990053112767498 1102 18-19 0.16561615867553314 1102 20-21 0.0682838105395902 1102 22-23 0.09975907110629834 1102 24-25 0.20760523986536583 1102 26-27 0.2056903162024284 1102 28-29 0.23337569949693204 1102 30-31 0.2430816482301168 1102 32-33 0.1917423703925678 1102 34-35 0.3054739021633708 1102 36-37 0.22358964498172895 1102 38-39 0.29550221641260777 1102 40-41 0.19285503549522076 1102 42-43 0.24786570013791476 1102 44-45 0.15870072063960095 1102 46-47 0.09657334790632177 1102 48-49 0.2129880904380741 1102 50-51 0.16280403252230258 1102 52-53 0.07856090039184949 1102 54-55 0.14194423087879215 1102 56-57 0.11350640333700568 1102 58-59 0.09496537331546051 1102 60-61 -0.06720203802979086 1102 62-63 -0.16183401987351687 1102 64-65 -0.12064889223553621 1102 66-67 -0.06127041567504676 1102 68-69 -0.10917874477347311 1102 70-71 -0.18244266211687687 1102 72-73 -0.3396856235570951 1102 74-75 -0.33442758162686204 1102 76-77 -0.44524130974687637 1102 78-79 -0.14721094100251975 1102 80-81 -0.22401670422169673 1102 82-83 -0.21622573225645425 1102 84-85 -0.2880857600663447 1102 86-87 -0.2089074115118308 1102 88-89 -0.3838244568073037 1102 90-91 -0.40677423015298686 1102 92-93 -0.35610286976258365 1102 94-95 -0.3527966399670035 1102 96-97 -0.34975992719066795 1102 98-99 -0.43559759401342646 1102 100-101 -1.6826242642643514 1103 1 0.11826803633945815 1103 2 0.22240882444231147 1103 3 0.11401522661177665 1103 4 0.15176544415850657 1103 5 0.07190427755516993 1103 6 0.10550872828680014 1103 7 0.13795576901309659 1103 8 0.10826631299488554 1103 9 0.0960063108797371 1103 10-11 0.14372321514207442 1103 12-13 0.15648482749745085 1103 14-15 0.23974796976512636 1103 16-17 0.14352631337105493 1103 18-19 0.0978737110079635 1103 20-21 0.21916915595416242 1103 22-23 0.2173421075794053 1103 24-25 0.2586844931041625 1103 26-27 0.22098348165575032 1103 28-29 0.28029262099146024 1103 30-31 0.27101273839026874 1103 32-33 0.1995980130156525 1103 34-35 0.29148868465185274 1103 36-37 0.32975987622847214 1103 38-39 0.2546523050245497 1103 40-41 0.13011755538533265 1103 42-43 0.15408791820348 1103 44-45 0.20965559681185653 1103 46-47 0.3768839422738992 1103 48-49 0.3187023082740268 1103 50-51 0.20673267943097073 1103 52-53 0.24492323561911178 1103 54-55 0.32046477373469884 1103 56-57 0.21836057042023072 1103 58-59 0.21228514320606706 1103 60-61 0.2035105060369915 1103 62-63 0.1576263255571817 1103 64-65 0.17933789606050254 1103 66-67 0.25464953709391835 1103 68-69 0.1417828931252103 1103 70-71 0.19520067304610222 1103 72-73 0.15642718998974203 1103 74-75 0.06845924556255056 1103 76-77 0.012189107358125284 1103 78-79 0.13011772125708632 1103 80-81 -0.031708743164934106 1103 82-83 -0.016040144903222142 1103 84-85 -0.04542841447719326 1103 86-87 -0.013099423902666274 1103 88-89 -0.04508899855037285 1103 90-91 -0.09958717069988765 1103 92-93 0.02097103988483795 1103 94-95 0.15236839453285 1103 96-97 -0.07650012575107823 1103 98-99 -0.12063858039558895 1103 100-101 -0.7260036418372202 1104 1 0.09620872590754459 1104 2 0.15783419515175723 1104 3 0.081099122676072 1104 4 0.13495204394938298 1104 5 0.05890836848736569 1104 6 0.04912597318680412 1104 7 0.08201922042625398 1104 8 0.06854151731049996 1104 9 0.06420129581112377 1104 10-11 0.09766148811112885 1104 12-13 0.11545399227894393 1104 14-15 0.1751965844074661 1104 16-17 0.14062539354655001 1104 18-19 0.12485361285023089 1104 20-21 0.11560742507327859 1104 22-23 0.1170047866202566 1104 24-25 0.18819690565307923 1104 26-27 0.18050997146191605 1104 28-29 0.15494651257444048 1104 30-31 0.16942486496661502 1104 32-33 0.2242735411871024 1104 34-35 0.2607928334869456 1104 36-37 0.2800646364319306 1104 38-39 0.29400240658561216 1104 40-41 0.27785637789293105 1104 42-43 0.25955974212939026 1104 44-45 0.20868426028667386 1104 46-47 0.21441419307353016 1104 48-49 0.3004844547776031 1104 50-51 0.3399796556984853 1104 52-53 0.37558137675127057 1104 54-55 0.3102185786173308 1104 56-57 0.349658232513697 1104 58-59 0.2589425501571867 1104 60-61 0.3165809785616034 1104 62-63 0.2721043313105085 1104 64-65 0.23956205997190239 1104 66-67 0.3249705670879237 1104 68-69 0.21778179512610052 1104 70-71 0.2622270800788371 1104 72-73 0.28878083887334327 1104 74-75 0.2503068793205543 1104 76-77 0.08540321295083686 1104 78-79 0.3446700498467976 1104 80-81 0.23456263547844003 1104 82-83 0.2135886413072683 1104 84-85 0.11718171267818889 1104 86-87 0.2863063014004368 1104 88-89 0.10954465781748013 1104 90-91 0.09175852115505378 1104 92-93 0.09118225071760833 1104 94-95 0.1884679066701267 1104 96-97 0.20337015674115122 1104 98-99 0.15740659618515096 1104 100-101 -0.3684554561537148 1105 1 0.04217823365225115 1105 2 0.14644758146808812 1105 3 0.0631916807275914 1105 4 0.07261457469844146 1105 5 0.04945624240945534 1105 6 0.06391593344955737 1105 7 0.08620312008294206 1105 8 0.11923725704504307 1105 9 0.05015404362828235 1105 10-11 0.08100816856418191 1105 12-13 0.05297916596045127 1105 14-15 0.08887332730477482 1105 16-17 0.07866434686204826 1105 18-19 0.1107394561178765 1105 20-21 0.06464829091277124 1105 22-23 0.1136982488969025 1105 24-25 0.12380685212944087 1105 26-27 0.16377031801395958 1105 28-29 0.1154613397038986 1105 30-31 0.14761246126862204 1105 32-33 0.157619038983988 1105 34-35 0.14360241908421756 1105 36-37 0.11705001204353493 1105 38-39 0.1634445983516244 1105 40-41 0.10901362385838098 1105 42-43 0.16023960048097052 1105 44-45 0.04671375235274411 1105 46-47 0.08856014636199205 1105 48-49 0.16751267633303257 1105 50-51 0.18490048531489833 1105 52-53 0.12727527830021756 1105 54-55 0.17947154539482568 1105 56-57 0.23879992823991358 1105 58-59 0.26627890433230306 1105 60-61 0.21935667261183767 1105 62-63 0.060646251403504436 1105 64-65 0.22933157717785946 1105 66-67 0.2812663527771164 1105 68-69 0.21588211140215208 1105 70-71 0.0904335988796916 1105 72-73 0.015722084514408152 1105 74-75 0.07774450265470279 1105 76-77 0.09338056677452755 1105 78-79 0.22553653134330176 1105 80-81 0.12631191912669948 1105 82-83 0.21426207952530518 1105 84-85 0.1646697509557029 1105 86-87 0.1906213069420275 1105 88-89 0.16618375170010324 1105 90-91 0.26990540029435195 1105 92-93 0.3474918903791675 1105 94-95 0.24385372290563367 1105 96-97 0.23025716914890282 1105 98-99 0.3516027954660963 1105 100-101 -0.6297187356930856 1106 1 0.1249864170316286 1106 2 0.1820395573759015 1106 3 0.11504090544697476 1106 4 0.10339557970105773 1106 5 0.1324660236384574 1106 6 0.14252665049079383 1106 7 0.09388872699202722 1106 8 0.06677826105210727 1106 9 0.10302121304513889 1106 10-11 0.08248779722592303 1106 12-13 0.11297817041551639 1106 14-15 0.2450681056715851 1106 16-17 0.11694118302023782 1106 18-19 0.04754786349487006 1106 20-21 0.15334512259100563 1106 22-23 0.16045152797303786 1106 24-25 0.12307450438559187 1106 26-27 0.20689110250337706 1106 28-29 0.22264792971360237 1106 30-31 0.19581736930516058 1106 32-33 0.1420132498901836 1106 34-35 0.1905081409787357 1106 36-37 0.24914015863753036 1106 38-39 0.20972934909206487 1106 40-41 0.29478915336384404 1106 42-43 0.3477940443446812 1106 44-45 0.33854219507157524 1106 46-47 0.22980059535276354 1106 48-49 0.3644159093652135 1106 50-51 0.2994273525838622 1106 52-53 0.25269154052674736 1106 54-55 0.26712616596775973 1106 56-57 0.2696195106088126 1106 58-59 0.2554156424293126 1106 60-61 0.31215079391898826 1106 62-63 0.384925725755771 1106 64-65 0.4240309226070593 1106 66-67 0.5210253064593857 1106 68-69 0.5039467720457722 1106 70-71 0.4262856609021455 1106 72-73 0.5058568564101762 1106 74-75 0.4306919378820524 1106 76-77 0.3547447122236491 1106 78-79 0.5374077824943981 1106 80-81 0.391016740053054 1106 82-83 0.42994502335168505 1106 84-85 0.5374671077838897 1106 86-87 0.5573831725932337 1106 88-89 0.6670218760934432 1106 90-91 0.5652991383166999 1106 92-93 0.6787358237772096 1106 94-95 0.808555432754062 1106 96-97 0.8605242240780946 1106 98-99 0.8125861449770362 1106 100-101 0.5882203618454298 1107 1 0.044649136928548216 1107 2 0.15928655321045682 1107 3 0.08714734243209676 1107 4 0.11891624880608731 1107 5 0.06573751601591482 1107 6 0.03293295497638127 1107 7 0.10483316536210907 1107 8 0.07673794686242985 1107 9 0.08009279689078141 1107 10-11 0.042001369904909325 1107 12-13 0.09504264725396183 1107 14-15 0.05443877401242503 1107 16-17 0.11229746804825425 1107 18-19 0.06791140836894272 1107 20-21 0.05143252055636793 1107 22-23 0.04656929149368949 1107 24-25 0.10114612467742035 1107 26-27 0.14862034492814757 1107 28-29 0.11017145808108353 1107 30-31 0.1278514547232703 1107 32-33 0.04634188166491526 1107 34-35 0.0808424936297314 1107 36-37 0.06215027304329368 1107 38-39 0.10462794050557278 1107 40-41 0.13285473064211573 1107 42-43 0.13436421090377593 1107 44-45 0.10537322230157997 1107 46-47 0.08242642582624171 1107 48-49 0.12096029318357182 1107 50-51 0.13611173680195776 1107 52-53 0.08542891985752021 1107 54-55 0.16001292765317743 1107 56-57 0.1809556867450226 1107 58-59 0.21742660215157628 1107 60-61 0.2361136940481927 1107 62-63 0.13092563875518692 1107 64-65 0.24142339752679476 1107 66-67 0.2560422186675666 1107 68-69 0.27505329015306046 1107 70-71 0.20358626790757484 1107 72-73 0.19034347654328343 1107 74-75 0.18515348068458337 1107 76-77 0.05415393122880374 1107 78-79 0.29609614287433317 1107 80-81 0.30596038366103784 1107 82-83 0.3991796180759266 1107 84-85 0.4126614776704045 1107 86-87 0.4817883648821564 1107 88-89 0.5844714892347724 1107 90-91 0.6081980683270807 1107 92-93 0.7256278454709246 1107 94-95 0.7562616153212574 1107 96-97 0.8270392275639971 1107 98-99 0.9773049391019093 1107 100-101 1.7316832672652147 1108 1 0.06481937903166823 1108 2 0.07258695561987594 1108 3 0.06362809213803189 1108 4 0.06747801456558733 1108 5 0.06307301552321576 1108 6 0.04874668040031338 1108 7 0.02506303050262204 1108 8 0.04026630344383619 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-0.44799607127117724 2316 56-57 -0.41386152533845433 2316 58-59 -0.3715348402263743 2316 60-61 -0.4899610611501828 2316 62-63 -0.5188841075586481 2316 64-65 -0.6156713336836219 2316 66-67 -0.38881230663886157 2316 68-69 -0.39909824028283936 2316 70-71 -0.4338238006625019 2316 72-73 -0.36881429463853266 2316 74-75 -0.4106348752148179 2316 76-77 -0.43109647049772803 2316 78-79 -0.29091810552673536 2316 80-81 -0.11055778012838857 2316 82-83 -0.21825125089716835 2316 84-85 -0.10798832866959174 2316 86-87 -0.20545663798976932 2316 88-89 -0.23256424708262102 2316 90-91 -0.10674122112165207 2316 92-93 -0.2525193561311063 2316 94-95 -0.2312365799562741 2316 96-97 -0.05759659975515774 2316 98-99 0.08704253401780804 2316 100-101 0.5914230497234989 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 14.0 5 18.0 6 74.0 7 139.0 8 412.0 9 891.0 10 1564.0 11 2378.0 12 3739.0 13 5043.0 14 6377.0 15 8080.0 16 10171.0 17 13035.0 18 12605.0 19 10416.0 20 10020.0 21 10350.0 22 11582.0 23 13850.0 24 16012.0 25 19208.0 26 24048.0 27 29712.0 28 36747.0 29 47373.0 30 60803.0 31 79211.0 32 105674.0 33 145604.0 34 212898.0 35 334699.0 36 562653.0 37 1051625.0 38 774450.0 39 37.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.588074688503134 26.374709284330976 14.311777632358533 23.725438394807355 2 12.317959171163182 22.633631450315324 44.75568057403695 20.29272880448454 3 15.225833601176966 32.93341793878586 30.937016431792653 20.903732028244526 4 9.86189209159921 19.60057611232423 44.47874867873012 26.058783117346433 5 10.759610063851714 39.6250078696325 37.79317832642462 11.822203740091167 6 23.766317752013244 43.73378441514548 21.305110898308886 11.194786934532388 7 22.50228909661037 33.41175353084178 28.802661647774023 15.283295724773824 8 22.358509530263017 38.61219997371267 22.294282283993773 16.735008212030543 9 25.504236347430194 15.371932638154851 24.8254046095613 34.29842640485366 10-11 21.858649803010675 28.648996718501312 31.263261021075152 18.22909245741286 12-13 22.24286735168366 26.365781622944645 31.203175686646144 20.18817533872555 14-15 20.394843478316098 27.69344066606452 28.21374834979101 23.69796750582837 16-17 18.55194067898637 29.704383468138758 30.315274045455 21.428401807419874 18-19 18.169697441623896 30.830362201906603 31.76509505963801 19.234845296831494 20-21 18.835302674349638 29.018330997295056 30.968066964221613 21.178299364133693 22-23 19.382117875497443 30.204878288539938 29.78487462156732 20.628129214395297 24-25 19.06287311722494 30.175746841930284 30.81325610600644 19.94812393483834 26-27 19.338406899210163 29.561970732698683 30.3541251785164 20.745497189574753 28-29 18.96673934341309 30.980423667878316 29.3788568102419 20.673980178466696 30-31 20.539865073079895 29.96383282581107 29.002785021521372 20.493517079587665 32-33 20.492315925153996 30.36979541164529 28.956091868709127 20.18179679449159 34-35 20.654706482036804 29.184504500325275 30.27852148105931 19.882267536578606 36-37 20.63051771688983 29.690080065917147 30.499340607635034 19.180061609557985 38-39 21.160238507983127 28.70974680755066 31.41716420259593 18.712850481870287 40-41 19.874483024281204 29.10759515080055 31.27973883190928 19.738182993008962 42-43 20.747552139741078 28.990182049337726 30.169653325881185 20.09261248504001 44-45 19.584850653704137 30.992932241635824 30.092770541397577 19.329446563262458 46-47 20.933305278783802 29.22335563338668 29.43446887988345 20.408870207946066 48-49 20.93696815182505 28.19521025826102 30.306622057287974 20.56119953262596 50-51 20.8115882961721 28.79464014517677 28.612009445768017 21.78176211288311 52-53 19.256134723690298 29.64830198182198 29.518632528477024 21.576930766010697 54-55 20.088465714358296 30.32782403833091 28.753552379721643 20.830157867589154 56-57 20.939725242136166 29.1213679575073 29.369799829684585 20.56910697067195 58-59 20.606232307133258 28.31140329077657 30.702763704481768 20.379600697608407 60-61 20.546574970261073 30.808313424543755 28.606583541257308 20.038528063937868 62-63 21.03417463846632 29.55160214672529 27.698013759983386 21.716209454824998 64-65 19.592029967560546 29.54358064412804 28.394628106019688 22.469761282291724 66-67 20.508015797572376 27.625428265118114 29.54796327706432 22.318592660245194 68-69 19.432732456214836 29.18517008927345 28.65506886428163 22.727028590230084 70-71 20.110236881446617 28.54386086991098 29.514101164332534 21.83180108430987 72-73 21.114845274741295 28.318030349720946 29.852553461036756 20.714570914501 74-75 20.660587996849937 29.588451355730584 28.60561709516125 21.145343552258225 76-77 19.912427391002282 29.363567430964153 30.004125417097892 20.71987976093567 78-79 19.567019510175925 29.342417629270486 29.470049906926743 21.620512953626847 80-81 19.525571027933122 29.598750636500498 30.360604457326023 20.515073878240354 82-83 20.733967121354688 28.09951268139829 30.74031144484201 20.42620875240501 84-85 22.126336246638413 28.569548888270962 28.604245117468757 20.699869747621864 86-87 20.223423625419922 29.891956550672568 29.029539092300276 20.855080731607238 88-89 20.850273752759893 28.85929538900284 29.089074851526263 21.201356006711002 90-91 20.652649332489485 27.77644776386464 30.53559614260989 21.035306761035987 92-93 20.57226240447463 29.064468158163276 28.965144275739885 21.398125161622215 94-95 18.89137656945475 28.90868754937661 29.8005523276765 22.399383553492143 96-97 19.70187904399826 28.964885558577087 28.585627831765255 22.7476075656594 98-99 18.63466846480866 29.972434196065954 29.199677704033338 22.193219635092042 100-101 18.23885616773114 29.149664090393234 29.085188222592233 23.526291519283394 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 44.0 1 36.5 2 34.0 3 40.5 4 36.5 5 38.0 6 62.0 7 107.0 8 178.0 9 228.0 10 235.0 11 261.0 12 328.5 13 440.0 14 564.5 15 693.0 16 839.0 17 1003.0 18 1185.5 19 1336.5 20 1529.0 21 1733.0 22 1915.0 23 2140.0 24 2455.0 25 2921.0 26 4035.5 27 6279.0 28 13629.5 29 34393.5 30 61151.5 31 83800.5 32 114330.5 33 150984.5 34 200311.0 35 236989.0 36 251578.0 37 278464.5 38 268293.0 39 205642.5 40 151465.5 41 115427.0 42 95191.5 43 86326.0 44 79531.0 45 83199.5 46 85932.0 47 88428.5 48 93509.5 49 89942.5 50 85195.5 51 85003.5 52 81010.5 53 76054.0 54 72679.0 55 64212.0 56 54842.5 57 44415.0 58 34949.5 59 33035.5 60 27700.5 61 19035.0 62 13252.0 63 10498.5 64 7750.0 65 3910.5 66 2107.5 67 1434.0 68 1110.5 69 866.5 70 693.5 71 546.0 72 416.0 73 332.0 74 259.0 75 215.0 76 188.5 77 147.5 78 105.5 79 76.0 80 57.5 81 48.0 82 41.5 83 30.5 84 19.5 85 16.5 86 19.5 87 16.0 88 8.0 89 6.0 90 6.5 91 4.5 92 2.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07897245242047805 2 1.9328921921096028E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.3806372800782878E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 5.52254912031315E-5 40-41 0.002581791713746398 42-43 0.005246421664297493 44-45 0.0 46-47 0.0 48-49 1.5187010080861164E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.002029536801715083 68-69 0.006254286878754643 70-71 0.0011597353152657615 72-73 0.0 74-75 0.0 76-77 0.001767215718500208 78-79 0.004832230480274007 80-81 0.004197137331437994 82-83 0.00465274763386383 84-85 0.0023470833761330893 86-87 2.7612745601565756E-4 88-89 0.0 90-91 0.0 92-93 8.421887408477555E-4 94-95 0.0031754657441800615 96-97 0.0027612745601565754 98-99 0.0 100-101 1.6567647360939453E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3621516.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.47723646732813 #Duplication Level Percentage of deduplicated Percentage of total 1 75.05583740029412 14.618802932988592 2 12.706302426888499 4.949673139877852 3 4.197743257603806 2.4528131417244485 4 2.050274753124138 1.5973474475836655 5 1.1013892106135732 1.0726009048842218 6 0.734575368273695 0.858449889056484 7 0.5078947076937217 0.692466972557858 8 0.3749159027839335 0.5841860555107581 9 0.28418053216438466 0.4981546281939179 >10 2.0797792565284414 8.28571393711431 >50 0.35713209448743155 4.943095146239143 >100 0.42020010726963214 17.91357217335097 >500 0.07246246256965926 9.783706785579028 >1k 0.050309244229822594 18.814739369925228 >5k 0.004716491646545869 5.893590989185824 >10k+ 0.0022867838286283 7.0410864862277105 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 30356 0.83821250548113 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 23954 0.661435708139906 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 20140 0.5561206964155342 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 19059 0.5262713184202417 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 18930 0.5227092742376397 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 16992 0.4691957732618053 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 15728 0.43429326282142616 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 15668 0.4326364980853322 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 12959 0.3578335702506906 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 12487 0.34480035432675155 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 12162 0.3358262120062427 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 11763 0.32480872651121795 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 10917 0.30144834373229334 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 10719 0.2959810201031833 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 10570 0.29186672100855 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 10530 0.2907622111844874 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 9287 0.25643956840174115 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 8502 0.23476356310451205 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 8253 0.22788798944972216 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 8233 0.22733573453769088 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 7457 0.2059082439508758 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 6991 0.1930407045005462 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 6908 0.19074884661561622 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 6796 0.18765621910824087 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 6587 0.18188515527751362 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 6458 0.17832311109491164 No Hit AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA 6407 0.17691486106923177 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 6407 0.17691486106923177 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 6287 0.17360133159704388 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 6253 0.17266249824659066 No Hit CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA 6241 0.17233114529937185 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 6241 0.17233114529937185 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 6220 0.17175127764173898 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 6195 0.17106095900169985 No Hit GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG 6188 0.17086766978248888 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 6033 0.16658769421424618 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 5987 0.16531750791657418 No Hit GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT 5956 0.16446151280292565 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 5936 0.1639092578908943 No Hit GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG 5935 0.16388164514529274 No Hit ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT 5905 0.16305326277724577 No Hit TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG 5858 0.16175546373397218 No Hit GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA 5845 0.16139649804115183 No Hit CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT 5840 0.161258434313144 No Hit CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC 5560 0.15352686554470557 No Hit CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC 5315 0.14676174287232197 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 5263 0.14532588010104056 No Hit GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT 5209 0.14383479183855602 No Hit TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG 5160 0.1424817673040793 No Hit ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA 4947 0.13660025249094576 No Hit AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA 4806 0.13270685536112503 No Hit TTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGC 4748 0.1311053161162342 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 4653 0.12848210528408546 No Hit ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT 4552 0.1256932179783273 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 4523 0.12489244835588191 No Hit ATAGAATACAACACCAACGTCGCAAAAAACCAGCAAAAATTCTTAACATT 4446 0.12276626694456133 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 4408 0.12171698261170184 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 4385 0.12108188946286583 No Hit TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT 4371 0.1206953110244439 No Hit GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG 4263 0.11771313449947482 No Hit CAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAAT 4001 0.11047859515186459 No Hit GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT 3988 0.11011962945904422 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 3969 0.10959498729261447 No Hit TCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCA 3846 0.10619861958362188 No Hit GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT 3797 0.10484559504914516 No Hit GATTTAACCTTTTATCCCTTATTAGAAAAAGTGGCAAAAACAGGCAAGCC 3790 0.1046523058299342 No Hit CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT 3763 0.10390676169869192 No Hit TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA 3735 0.1031336048218481 No Hit ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG 3708 0.10238806069060583 No Hit CTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCAC 3708 0.10238806069060583 No Hit GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC 3690 0.10189103126977764 No Hit CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC 3659 0.10103503615612908 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 3643 0.10059323222650404 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 3641 0.1005380067353009 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.7612745601565754E-5 2 0.0 0.0 0.0 0.0 2.7612745601565754E-5 3 0.0 0.0 0.0 0.0 2.7612745601565754E-5 4 0.0 0.0 0.0 0.0 2.7612745601565754E-5 5 0.0 0.0 0.0 0.0 2.7612745601565754E-5 6 0.0 0.0 0.0 0.0 5.522549120313151E-5 7 0.0 0.0 0.0 0.0 5.522549120313151E-5 8 0.0 0.0 0.0 0.0 5.522549120313151E-5 9 0.0 0.0 0.0 0.0 8.283823680469727E-5 10-11 0.0 0.0 0.0 0.0 8.283823680469727E-5 12-13 0.0 0.0 0.0 0.0 8.283823680469727E-5 14-15 0.0 0.0 0.0 0.0 8.283823680469727E-5 16-17 0.0 0.0 0.0 0.0 8.283823680469727E-5 18-19 0.0 0.0 0.0 0.0 8.283823680469727E-5 20-21 0.0 0.0 0.0 2.7612745601565754E-5 9.664460960548014E-5 22-23 0.0 0.0 0.0 9.664460960548014E-5 1.1045098240626302E-4 24-25 0.0 0.0 0.0 1.794828464101774E-4 1.1045098240626302E-4 26-27 0.0 0.0 0.0 4.2799755682426915E-4 1.1045098240626302E-4 28-29 0.0 0.0 0.0 0.0015739264992892478 1.1045098240626302E-4 30-31 0.0 0.0 0.0 0.007552085922028233 1.242573552070459E-4 32-33 0.0 0.0 0.0 0.026715331369514865 1.3806372800782875E-4 34-35 0.0 0.0 0.0 0.05892559911374132 1.6567647360939453E-4 36-37 0.0 0.0 0.0 0.10976066376622387 1.6567647360939453E-4 38-39 0.0 0.0 0.0 0.18775286371784633 1.6567647360939453E-4 40-41 0.0 0.0 0.0 0.2702597475753248 1.6567647360939453E-4 42-43 0.0 0.0 0.0 0.3694309234033482 1.6567647360939453E-4 44-45 0.0 0.0 0.0 0.4785703003935369 1.7948284641017742E-4 46-47 0.0 0.0 0.0 0.6138313347228066 1.9328921921096028E-4 48-49 0.0 0.0 0.0 0.7549186583740068 1.9328921921096028E-4 50-51 0.0 0.0 0.0 0.9027711046975908 2.2090196481252603E-4 52-53 0.0 0.0 0.0 1.0537023721557492 2.2090196481252603E-4 54-55 0.0 0.0 0.0 1.2134697182064085 2.2090196481252603E-4 56-57 0.0 0.0 0.0 1.407186934974193 2.2090196481252603E-4 58-59 0.0 0.0 0.0 1.64221281916192 2.2090196481252603E-4 60-61 0.0 0.0 0.0 1.8869583898013982 2.2090196481252603E-4 62-63 0.0 0.0 0.0 2.126429926030977 2.2090196481252603E-4 64-65 0.0 0.0 0.0 2.380978021359011 2.2090196481252603E-4 66-67 0.0 0.0 0.0 2.6580166979795203 2.2090196481252603E-4 68-69 0.0 0.0 0.0 2.964573399648103 2.2090196481252603E-4 70-71 0.0 0.0 0.0 3.2835834495829923 2.2090196481252603E-4 72-73 0.0 0.0 0.0 3.6533319195607588 2.2090196481252603E-4 74-75 0.0 0.0 0.0 3.993700428218459 2.2090196481252603E-4 76-77 0.0 0.0 0.0 4.373624194950402 2.2090196481252603E-4 78-79 0.0 0.0 0.0 4.78466752597531 2.2090196481252603E-4 80-81 0.0 0.0 0.0 5.270279076497246 2.2090196481252603E-4 82-83 0.0 0.0 0.0 5.799132186631234 2.3470833761330892E-4 84-85 0.0 0.0 0.0 6.297694114840304 2.485147104140918E-4 86-87 0.0 0.0 0.0 6.8393043134422165 2.485147104140918E-4 88-89 0.0 0.0 0.0 7.414394966086026 2.485147104140918E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2305 0.0 56.255627 2 ACCTGGG 2450 0.0 52.150734 3 CCTGGGG 2140 0.0 51.493027 4 CTGGGGG 1975 0.0 51.46606 5 GTACACG 330 0.0 46.094826 1 GTACCTG 2880 0.0 45.3896 1 ACGGGGG 330 0.0 35.983242 5 TATCCGC 70 2.0452595E-4 33.95378 1 TGGGGGG 7650 0.0 32.90703 6 TATTCGC 690 0.0 32.353626 9 TATTCTC 3905 0.0 30.043474 5 TCTCTGC 3910 0.0 29.519144 8 GTACCCG 130 3.0202318E-7 29.252485 1 GGGGGGA 8010 0.0 28.344553 7 GTACTAG 2070 0.0 27.786327 1 AGGGCTA 7170 0.0 27.293064 5 TTCTCTG 4285 0.0 27.157482 7 CATGGGG 8340 0.0 27.109102 4 ATGGGGG 7385 0.0 26.498478 5 CTGTGCG 2700 0.0 26.035875 9 >>END_MODULE