FastQCFastQC Report
Sat 18 Jun 2016
SRR1931056_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931056_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2429624
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA114920.47299499840304504No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC91730.37754813090420575No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA85490.3518651445655789No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC72030.2964656259569382No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC68950.28378876731543645No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC67910.279508269592332No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC50660.20850962947353172No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC49590.20410565585456844No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG49580.20406449722261555No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA46300.19056446594205523No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA42960.1768174828697774No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC39140.161094885463759No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC38710.1593250642897831No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT38040.15656743594893693No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA36850.15166955874653856No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG36650.15084638610748No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC34510.14203843886955347No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT34200.1407625212790127No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC29920.12314662680315966No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA29190.12014204667059594No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT28700.1181252737049025No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA27720.11409172777351557No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA26830.11042860952970501No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT25780.1061069531746476No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT25470.10483103558410684No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC24780.10199108997935483No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG24690.10162066229177848No Hit
GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG24370.1003035860692848No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG23900.047.366832
GTACCTG26050.044.3666271
ACCTGGG25600.043.295353
CCTGGGG24700.033.750574
CTGGGGG22250.032.7835585
GTACACG2950.032.1131021
TGGGGGG49950.029.4910536
CATGGGG46950.027.8444634
GTACTAG11750.027.4122891
AGGGCTA38500.026.3280325
GCTATTG39000.025.9904948
CTATTGA40150.025.4825279
ATGGGGG40750.025.4555115
GGGGGGA49650.025.1854697
GTACATG80650.025.0195521
ACATGGG79550.024.710033
TAGGCAT16650.023.8962865
TACATGG83450.023.8962672
GGGGAAT52700.023.4587149
GGGCTAT44650.023.444246
Produced by FastQC (version 0.11.5)