##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1931056_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2429624
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244293767266047	33.0	33.0	33.0	27.0	33.0
2	31.161657112376236	33.0	33.0	33.0	27.0	33.0
3	31.18790109086838	33.0	33.0	33.0	27.0	33.0
4	34.816918173346984	37.0	37.0	37.0	33.0	37.0
5	34.79712169455027	37.0	37.0	37.0	33.0	37.0
6	34.89633210735488	37.0	37.0	37.0	33.0	37.0
7	34.864353908259055	37.0	37.0	37.0	33.0	37.0
8	34.862931877525085	37.0	37.0	37.0	33.0	37.0
9	34.877183053838785	37.0	37.0	37.0	33.0	37.0
10-11	34.78069816564209	37.0	37.0	37.0	33.0	37.0
12-13	34.700836631511706	37.0	37.0	37.0	33.0	37.0
14-15	36.360566490946745	40.0	37.0	40.0	33.0	40.0
16-17	36.306452150620835	40.0	37.0	40.0	33.0	40.0
18-19	36.24253649947482	40.0	37.0	40.0	33.0	40.0
20-21	36.153589403133985	40.0	37.0	40.0	33.0	40.0
22-23	36.06598202026322	40.0	37.0	40.0	33.0	40.0
24-25	35.942361246019956	40.0	37.0	40.0	30.0	40.0
26-27	35.77836508858984	40.0	37.0	40.0	27.0	40.0
28-29	35.66127804137595	40.0	37.0	40.0	27.0	40.0
30-31	35.51806699308206	40.0	37.0	40.0	27.0	40.0
32-33	35.35332956869046	37.0	37.0	40.0	27.0	40.0
34-35	35.23088058069891	37.0	37.0	40.0	27.0	40.0
36-37	35.018713595190036	37.0	37.0	40.0	27.0	40.0
38-39	34.83380494265779	37.0	37.0	40.0	27.0	40.0
40-41	34.611559854528934	37.0	37.0	40.0	22.0	40.0
42-43	34.424004907755275	37.0	35.0	40.0	22.0	40.0
44-45	34.15417694260511	37.0	33.0	40.0	22.0	40.0
46-47	33.9884550037372	37.0	33.0	40.0	22.0	40.0
48-49	33.97198887564495	37.0	33.0	40.0	18.5	40.0
50-51	33.18686060065261	37.0	33.0	38.5	15.0	40.0
52-53	33.30961889576329	37.0	33.0	38.5	18.5	40.0
54-55	33.8026260441945	37.0	33.0	40.0	22.0	40.0
56-57	33.751577816155915	37.0	33.0	40.0	15.0	40.0
58-59	33.60366974478355	37.0	33.0	40.0	15.0	40.0
60-61	33.61838436729305	37.0	33.0	40.0	15.0	40.0
62-63	33.4529377796729	37.0	33.0	40.0	15.0	40.0
64-65	33.241496420845365	37.0	33.0	40.0	15.0	40.0
66-67	33.002024593105766	37.0	33.0	40.0	15.0	40.0
68-69	32.765776721007036	37.0	33.0	40.0	10.5	40.0
70-71	32.53280322387333	37.0	33.0	40.0	6.0	40.0
72-73	31.951889469317063	37.0	33.0	37.0	2.0	40.0
74-75	31.7783126113341	37.0	33.0	37.0	2.0	40.0
76-77	31.42632625459742	37.0	33.0	37.0	2.0	40.0
78-79	31.17193812705176	37.0	33.0	37.0	2.0	38.5
80-81	30.907475189576658	37.0	33.0	37.0	2.0	37.0
82-83	30.631979886599737	37.0	33.0	37.0	2.0	37.0
84-85	30.254167517278393	37.0	33.0	37.0	2.0	37.0
86-87	30.0867387299434	37.0	33.0	37.0	2.0	37.0
88-89	29.88648943210966	33.0	33.0	37.0	2.0	37.0
90-91	29.610359668821182	33.0	30.0	37.0	2.0	37.0
92-93	29.332754162784035	33.0	27.0	37.0	2.0	37.0
94-95	29.089462196619724	33.0	27.0	37.0	2.0	37.0
96-97	28.80819727661564	33.0	27.0	37.0	2.0	37.0
98-99	28.49271759745541	33.0	27.0	37.0	2.0	37.0
100-101	27.813734141579108	33.0	27.0	37.0	2.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.0020054047613413672
1101	2	0.0937157674940785
1101	3	0.07125200419600475
1101	4	0.13998574477149361
1101	5	0.10963887750617829
1101	6	0.07215184852606171
1101	7	0.15056573890914393
1101	8	0.014751867154082277
1101	9	0.07346130950042351
1101	10-11	0.21760204502393066
1101	12-13	0.21492557334860152
1101	14-15	0.2589258616731769
1101	16-17	0.19458009301276746
1101	18-19	0.225214789206845
1101	20-21	0.2990556676821754
1101	22-23	0.22915791761157323
1101	24-25	0.3489342113505103
1101	26-27	0.4937590792712001
1101	28-29	0.2954025718704685
1101	30-31	0.36781109825622593
1101	32-33	0.28002380295273355
1101	34-35	0.3648982883394041
1101	36-37	0.43585501587167386
1101	38-39	0.5857239390208449
1101	40-41	0.5319516760150691
1101	42-43	0.48007497411487776
1101	44-45	0.3706879712072393
1101	46-47	0.27674606091233045
1101	48-49	0.4049598458890671
1101	50-51	0.3404471533180029
1101	52-53	0.5296652643750122
1101	54-55	0.6226474162116844
1101	56-57	0.4516660970351012
1101	58-59	0.5583050270541392
1101	60-61	0.5236759415114207
1101	62-63	0.5759884300981284
1101	64-65	0.6268574769388309
1101	66-67	0.600476999856447
1101	68-69	0.6641961341194289
1101	70-71	0.5231767453404643
1101	72-73	0.5796334058371322
1101	74-75	0.5644543742353569
1101	76-77	0.32241192481506076
1101	78-79	0.29817729254130043
1101	80-81	0.3643469481317787
1101	82-83	0.43825344900134056
1101	84-85	0.5283962703534932
1101	86-87	0.35395813351024685
1101	88-89	0.36205488599166813
1101	90-91	0.23411711008712288
1101	92-93	0.28815274721126016
1101	94-95	0.21933784350005325
1101	96-97	0.14704466553861195
1101	98-99	0.18991562511340732
1101	100-101	0.20515142843732903
1102	1	0.02190950450791007
1102	2	0.06739746399563273
1102	3	-0.10520065846543147
1102	4	-0.0062981770856254116
1102	5	-0.10291706290578873
1102	6	-0.07967477195783346
1102	7	0.07045447113081593
1102	8	-0.04268233261704779
1102	9	0.011437186155646373
1102	10-11	0.06005851878558133
1102	12-13	0.07471016444718259
1102	14-15	0.21476895264915186
1102	16-17	0.09844049230980545
1102	18-19	0.1253978088651735
1102	20-21	0.06031335629587886
1102	22-23	0.10566856417034387
1102	24-25	0.11250767975823095
1102	26-27	-0.10229124666471989
1102	28-29	0.04596593524303927
1102	30-31	0.012301095395343964
1102	32-33	0.13640356427364253
1102	34-35	0.051040775465445165
1102	36-37	0.17210453054389774
1102	38-39	0.07113128228552057
1102	40-41	0.1816378732931554
1102	42-43	0.32788946651311335
1102	44-45	0.3739277895413835
1102	46-47	0.07738490552571164
1102	48-49	0.07788200442266202
1102	50-51	0.21701757182380277
1102	52-53	0.3304361692303388
1102	54-55	0.3748714945997804
1102	56-57	0.4508838252924221
1102	58-59	0.42784470643128714
1102	60-61	0.5802572415768168
1102	62-63	0.612792751047941
1102	64-65	0.37614481141651623
1102	66-67	0.39238454236095066
1102	68-69	0.47936326567901943
1102	70-71	0.3823296440244661
1102	72-73	0.12354496889909328
1102	74-75	0.25083901437758627
1102	76-77	0.13827039301843058
1102	78-79	0.25998608361535247
1102	80-81	0.2586632490145675
1102	82-83	0.20518410843407153
1102	84-85	0.20180337350863553
1102	86-87	0.1076555648491393
1102	88-89	0.16851498183113378
1102	90-91	0.22729404015905175
1102	92-93	0.1646971112786133
1102	94-95	0.18246225599801136
1102	96-97	0.23046242036123843
1102	98-99	0.21407754067659113
1102	100-101	0.285514300351668
1103	1	-0.07371899247655733
1103	2	-0.1644767542214467
1103	3	-0.19542750759242722
1103	4	-0.10652522932498698
1103	5	-0.14890215902244108
1103	6	-0.0331328284554786
1103	7	-0.004129758996860744
1103	8	0.004890691413429238
1103	9	-0.20210945015522697
1103	10-11	-0.03381431142092595
1103	12-13	0.09589455601496155
1103	14-15	0.11643734957452523
1103	16-17	0.1083759961460018
1103	18-19	0.04303060954963911
1103	20-21	0.10956285610314609
1103	22-23	0.11742329297996434
1103	24-25	0.026619447700753085
1103	26-27	0.03498452812338115
1103	28-29	0.04050700513400329
1103	30-31	0.16938329268774055
1103	32-33	0.10703248916338026
1103	34-35	-0.10702651680252018
1103	36-37	-0.0851446981425994
1103	38-39	0.06649176223319131
1103	40-41	0.04546050279496683
1103	42-43	0.07797225191850998
1103	44-45	0.1251712822433717
1103	46-47	0.061455761160509326
1103	48-49	0.1385870416778019
1103	50-51	0.24707717503353166
1103	52-53	0.2784394035094948
1103	54-55	0.2700747564474497
1103	56-57	0.316509362313532
1103	58-59	0.38072206695641597
1103	60-61	0.43380831897381
1103	62-63	0.4823473712723185
1103	64-65	0.48232077382110106
1103	66-67	0.44814185366442416
1103	68-69	0.305414069787183
1103	70-71	0.27444320163797187
1103	72-73	0.15486546629160713
1103	74-75	0.3045729613689083
1103	76-77	0.20986636098692202
1103	78-79	0.40045015201081924
1103	80-81	0.4178289455724489
1103	82-83	0.33382418442091577
1103	84-85	0.3648773856008951
1103	86-87	0.3431936791128507
1103	88-89	0.3577504372170459
1103	90-91	0.28925746121943163
1103	92-93	0.24505760606033888
1103	94-95	0.20779698631841725
1103	96-97	0.26018243795213536
1103	98-99	0.4085592287274906
1103	100-101	0.4229952414511331
1104	1	0.006983956940786129
1104	2	0.040513196572092625
1104	3	4.120219265004721E-4
1104	4	0.10715773058709743
1104	5	0.075297122187024
1104	6	0.0092373981714573
1104	7	0.059887547419776865
1104	8	0.017127753678906288
1104	9	0.006127532904677935
1104	10-11	0.025235023747335106
1104	12-13	-6.695064386263994E-4
1104	14-15	0.03587399997105223
1104	16-17	0.15944933060141153
1104	18-19	0.18887281225649133
1104	20-21	0.19720837158288873
1104	22-23	0.14256660555483336
1104	24-25	0.10212680886824188
1104	26-27	0.049868343455599984
1104	28-29	-0.013215557562148206
1104	30-31	0.06446225073849376
1104	32-33	0.07275429940663258
1104	34-35	0.17087235748834217
1104	36-37	0.17588715239649133
1104	38-39	0.1165074053328965
1104	40-41	0.27985393665336744
1104	42-43	0.12632497411487975
1104	44-45	0.23935264319305105
1104	46-47	0.26462061764991773
1104	48-49	0.24377965971885374
1104	50-51	0.25081062849530866
1104	52-53	0.27406686011970294
1104	54-55	0.3659962637294072
1104	56-57	0.4936064782407712
1104	58-59	0.35814877705414716
1104	60-61	0.5329689379653217
1104	62-63	0.425196098537846
1104	64-65	0.40455694502393413
1104	66-67	0.40498719489190904
1104	68-69	0.46849467135346856
1104	70-71	0.5083152648440148
1104	72-73	0.6978127054825229
1104	74-75	0.5113592944481233
1104	76-77	0.4918888751696713
1104	78-79	0.3995203776476828
1104	80-81	0.5319732779190147
1104	82-83	0.4435271635403488
1104	84-85	0.4915257029776079
1104	86-87	0.5705871228719488
1104	88-89	0.5253715969845771
1104	90-91	0.5817024381013063
1104	92-93	0.6087688819630337
1104	94-95	0.5559213984645943
1104	96-97	0.6011415183754885
1104	98-99	0.6407378768864582
1104	100-101	0.799244956806124
1105	1	0.10595873025975777
1105	2	0.11417330968817296
1105	3	0.13919108647448297
1105	4	0.12366190510904573
1105	5	0.008827959784653672
1105	6	0.10016477046699634
1105	7	0.0886854646475399
1105	8	0.05684047474902343
1105	9	0.0968196955763716
1105	10-11	0.07944276232351655
1105	12-13	0.09465592672412981
1105	14-15	0.10989500724279821
1105	16-17	0.009249133097149809
1105	18-19	0.08196914047267256
1105	20-21	0.14101571515053735
1105	22-23	0.17827447879301417
1105	24-25	0.09908254995810495
1105	26-27	0.11703505500959466
1105	28-29	0.06877744001393182
1105	30-31	0.0220634716739454
1105	32-33	0.03055122911307251
1105	34-35	0.030625477157968817
1105	36-37	-0.009421223579806792
1105	38-39	0.025409461172742454
1105	40-41	0.23279226145810838
1105	42-43	0.022822864410237287
1105	44-45	0.19299480137600966
1105	46-47	0.03569252715706028
1105	48-49	0.177148005171766
1105	50-51	0.283195043613361
1105	52-53	0.22941473694885417
1105	54-55	0.4916017938488082
1105	56-57	0.387989277414853
1105	58-59	0.38127113992333506
1105	60-61	0.4819894963637452
1105	62-63	0.315917886849185
1105	64-65	0.3573031520443166
1105	66-67	0.2913459344556131
1105	68-69	0.26217543264262844
1105	70-71	0.38676126538265976
1105	72-73	0.2373898668076002
1105	74-75	0.2952923225475992
1105	76-77	0.17201635519480618
1105	78-79	0.15982022503074944
1105	80-81	0.17698539749887132
1105	82-83	0.15622219900134482
1105	84-85	0.36861251507923143
1105	86-87	0.37127749005033195
1105	88-89	0.41615892080179506
1105	90-91	0.2838830647284709
1105	92-93	0.2518526417260283
1105	94-95	0.24935168843676436
1105	96-97	0.26022043030654274
1105	98-99	0.06854827279273223
1105	100-101	0.06007627020948192
1106	1	-0.016683105109773777
1106	2	-0.05053691833635199
1106	3	-0.016986599172174266
1106	4	0.09321600028833643
1106	5	-0.06654252493284929
1106	6	-0.09661811372713203
1106	7	-0.07442337258911635
1106	8	-0.02151410264847442
1106	9	-0.09719962255532266
1106	10-11	-0.05093018494122248
1106	12-13	-0.022941913585192708
1106	14-15	0.06794698536656085
1106	16-17	-0.00913470083160206
1106	18-19	-0.02028372269791845
1106	20-21	0.03419739967986146
1106	22-23	0.036580246299152464
1106	24-25	-0.009096966059480849
1106	26-27	0.01317418525262326
1106	28-29	0.04054176321774605
1106	30-31	-0.016892371546333607
1106	32-33	-0.19342277079871195
1106	34-35	-0.18322616723551022
1106	36-37	-0.18685749138732888
1106	38-39	-0.09440908217543864
1106	40-41	-0.10122286666785385
1106	42-43	-0.20235098407327712
1106	44-45	-0.1942519605581552
1106	46-47	-0.20930758310625208
1106	48-49	-0.19279440062661735
1106	50-51	-0.08877032635679427
1106	52-53	-0.11742750565982618
1106	54-55	2.0802887137705284E-4
1106	56-57	0.09234100266808554
1106	58-59	0.010822993082022947
1106	60-61	-0.07512450854664365
1106	62-63	-0.10793923395529958
1106	64-65	-0.026507070099498264
1106	66-67	0.0675919098448361
1106	68-69	-0.005030237831796569
1106	70-71	-0.014431232301802766
1106	72-73	-0.20157939178191597
1106	74-75	-0.02184516844756601
1106	76-77	-0.18295247704324424
1106	78-79	-0.04205572139598246
1106	80-81	0.0354651972607023
1106	82-83	0.12798468448334077
1106	84-85	0.11115614259507467
1106	86-87	0.010235354764603244
1106	88-89	0.10784194319259655
1106	90-91	0.039540491329862704
1106	92-93	0.12808814210092478
1106	94-95	0.2619926416998233
1106	96-97	0.18444031739691624
1106	98-99	0.2992053318497625
1106	100-101	0.2601075114803031
1107	1	-0.25457689006713835
1107	2	-0.18910335151571545
1107	3	-0.1856032215122312
1107	4	-0.23066391103999706
1107	5	-0.36881252241439455
1107	6	-0.274486935561832
1107	7	-0.27430270827115777
1107	8	-0.21924998616866276
1107	9	-0.25467486007860884
1107	10-11	-0.14824882868536093
1107	12-13	-0.1950544455155736
1107	14-15	-0.15335943419655962
1107	16-17	-0.17366644855462
1107	18-19	-0.20752841803435018
1107	20-21	-0.2590995729188279
1107	22-23	-0.15526010595209527
1107	24-25	-0.19112382909163728
1107	26-27	-0.28906934772112436
1107	28-29	-0.31519466965719545
1107	30-31	-0.4541536720641375
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2316	3	-0.6323745879203813
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2316	6	-0.6402442111745259
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2316	10-11	-0.4838716058272112
2316	12-13	-0.6064618881689583
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2316	16-17	-0.7907409334872924
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2316	80-81	-0.5480076109048007
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2316	86-87	-0.438794152799062
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2316	92-93	-0.4857603126704255
2316	94-95	-0.3801046328068374
2316	96-97	-0.3085289679577059
2316	98-99	-0.3090942067711744
2316	100-101	-0.3034903302853529
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	63455.0
3	9696.0
4	7551.0
5	6549.0
6	6575.0
7	7150.0
8	7355.0
9	7629.0
10	8016.0
11	8301.0
12	9043.0
13	9640.0
14	10033.0
15	11301.0
16	13225.0
17	16727.0
18	14255.0
19	10999.0
20	11019.0
21	11671.0
22	12882.0
23	14405.0
24	16487.0
25	19086.0
26	21526.0
27	25507.0
28	30285.0
29	36687.0
30	45320.0
31	56499.0
32	73452.0
33	96624.0
34	133380.0
35	195168.0
36	304743.0
37	538280.0
38	558531.0
39	572.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.845113320423195	23.100697024014096	13.054496288506614	22.9996933670561
2	14.12620202350313	21.633644762875743	43.37302931531737	20.86712389830376
3	16.99544908444568	30.764715978335715	30.15161623973451	22.088218697484095
4	11.103880842986152	18.2115911213405	43.05283048533484	27.63169755033851
5	11.636319782809661	38.8643441195864	36.78229187503653	12.717044222567415
6	25.815146705827736	41.213990312904386	20.75271729288153	12.218145688386352
7	23.9920251034728	33.04021527610856	26.923301712528357	16.044457907890276
8	23.445026884818393	36.85718448615918	22.387332360892056	17.310456268130377
9	25.783989621439368	15.81483390022489	23.70086894103779	34.70030753729795
10-11	22.9480505716423	27.65884412084593	30.537562934159524	18.85554237335224
12-13	23.420665794515074	25.687313281255303	30.368511829096157	20.523509095133463
14-15	21.109871275726128	27.04041186499139	27.915065193572104	23.93465166571038
16-17	19.83328373499753	29.289978213776646	29.25435384893376	21.62238420229206
18-19	19.568631905976723	29.688363079876485	30.840233856316008	19.902771157830788
20-21	20.486729633885737	28.41993246691669	30.082720618498993	21.010617280698575
22-23	20.61277794424158	29.004179247488498	29.290231739561346	21.092811068708574
24-25	20.349609651534557	29.092423354395574	29.974329361250955	20.583637632818906
26-27	20.53507044711445	28.66184232622002	29.696920181888224	21.106167044777298
28-29	20.171084085438736	29.547637823794958	29.24193208496459	21.039346005801722
30-31	21.246044655469323	28.85479399281535	28.912868822500933	20.9862925292144
32-33	21.156874479343305	29.265906988077166	28.74397437628209	20.833244156297432
34-35	21.243636875500076	28.52556609582388	29.69185767015802	20.53893935851803
36-37	21.285659838724015	28.712220491730406	29.647797354652404	20.35432231489317
38-39	21.566526343170793	28.309668491914795	30.055844031833733	20.067961133080676
40-41	20.91897947785229	28.25325699214568	30.053271726966248	20.774491803035787
42-43	21.275395138382553	28.364168357772307	29.296288087434124	21.06414841641102
44-45	20.83664404782411	29.4990166123267	29.19355625374674	20.470783086102447
46-47	21.442080922028627	28.493731592075523	28.852932844866046	21.2112546410298
48-49	21.606761121277604	27.960668914693027	29.278068340883745	21.154501623145627
50-51	21.3491785424479	28.344913011933897	28.370322749110453	21.93558569650775
52-53	20.545721666341837	28.92481327814537	28.705512134855958	21.82395292065684
54-55	20.943058286880735	29.248766413584125	28.36955610707336	21.43861919246178
56-57	21.543894972198935	28.50499577133248	28.61039257471575	21.340716681752838
58-59	21.384438660316786	28.061019795089987	29.36469805450233	21.189843490090897
60-61	21.264240340152575	29.471017517477964	28.32921114091708	20.93553100145238
62-63	21.398749285615544	28.78339038092939	27.8309946913756	21.98686564207946
64-65	20.484937912770675	28.859920969199116	28.28455132081459	22.370589797215622
66-67	21.22142245341528	27.688059359110405	28.72774734750528	22.36277083996904
68-69	20.64928234500653	28.447188058814916	28.32294165327745	22.580587942901104
70-71	21.076166860258958	27.899361712704913	28.872727662781	22.151743764255126
72-73	21.77936489173961	27.754745270282882	29.17329749884388	21.292592339133627
74-75	21.460053757380262	28.578242429444735	28.32581646994582	21.635887343229186
76-77	21.008918823018437	28.33412483867989	29.09811036551814	21.558845972783534
78-79	20.702351433401244	28.433424575961297	28.914291188902386	21.949932801735077
80-81	20.66926529701635	28.42150645574654	29.463160008527723	21.446068238709383
82-83	21.45259597452372	27.493018290078442	29.869358351247588	21.18502738415025
84-85	22.11423869481107	27.924736889018003	28.484673042513712	21.47635137365721
86-87	20.95991032646308	28.609761073409068	28.93947716978007	21.490851430347785
88-89	21.53134604407979	27.87366155848051	28.798593314904007	21.79639908253569
90-91	21.349536435317564	27.337154080070636	29.686851432367416	21.62645805224438
92-93	21.51996256741505	27.945800289570492	28.80172628721425	21.732510855800204
94-95	20.279118247578083	27.92093683720961	29.48724556270595	22.312699352506357
96-97	20.82956305738863	27.88892092178069	28.760630014398313	22.520886006432363
98-99	20.230940237840127	28.506171671663317	28.853989622548383	22.408898467948173
100-101	20.69984562319819	27.624812644760453	28.809171849139798	22.86616988290156
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	82.0
1	55.5
2	47.0
3	63.5
4	50.5
5	46.5
6	75.5
7	133.5
8	223.0
9	270.0
10	276.5
11	284.0
12	366.0
13	523.0
14	635.0
15	795.0
16	1011.0
17	1178.0
18	1369.0
19	1600.0
20	1824.0
21	2097.0
22	2456.0
23	2918.0
24	3462.5
25	4308.5
26	6081.5
27	9199.5
28	15456.0
29	28708.5
30	45567.5
31	60316.0
32	77459.5
33	94483.5
34	112528.5
35	125591.0
36	130418.0
37	138583.0
38	130938.0
39	104970.0
40	84060.5
41	70000.0
42	62126.0
43	59706.5
44	59705.5
45	63694.0
46	67695.0
47	70681.0
48	73138.5
49	71531.5
50	69043.0
51	67493.0
52	63691.0
53	60381.0
54	57848.5
55	51951.5
56	46297.5
57	39368.0
58	32410.0
59	29942.5
60	25689.5
61	20090.0
62	15959.0
63	13070.0
64	10306.0
65	7293.5
66	5485.5
67	4369.0
68	3542.0
69	2941.5
70	2445.0
71	1970.0
72	1636.0
73	1390.5
74	1185.5
75	987.0
76	788.0
77	643.0
78	532.5
79	429.5
80	342.5
81	287.5
82	229.5
83	167.0
84	132.5
85	110.5
86	86.5
87	68.0
88	53.0
89	52.0
90	50.5
91	41.0
92	24.0
93	13.5
94	12.0
95	8.0
96	6.0
97	4.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.7042768757634926E-4
2	0.003086897396469577
3	1.234758958587831E-4
4	9.46648534917337E-4
5	0.0020579315976463845
6	0.0
7	0.0
8	0.0
9	0.0
10-11	6.173794792939155E-5
12-13	2.0579315976463846E-5
14-15	0.0037042768757634924
16-17	0.004403973618963264
18-19	0.004033545931386914
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	2.057931597646385E-4
42-43	2.0579315976463846E-5
44-45	5.144828994115961E-4
46-47	0.6902508371665739
48-49	0.4978548121026134
50-51	0.3824460081066042
52-53	0.32268367451095314
54-55	0.313669934113262
56-57	0.28296559467637794
58-59	0.28407687773910695
60-61	0.2617071612726908
62-63	0.25555394579572804
64-65	0.25265226224304665
66-67	0.24151885229977973
68-69	0.22262704023338592
70-71	0.23524216092695827
72-73	0.7630398777753266
74-75	0.5054897383298814
76-77	0.5924785069623942
78-79	0.3316768355926678
80-81	0.2855380091734359
82-83	0.2678192181177005
84-85	0.25979328488687964
86-87	0.23544795408672287
88-89	0.23746472705241636
90-91	0.24752801256490717
92-93	0.2057314218167091
94-95	0.1758296757029071
96-97	0.135761747496732
98-99	0.14399347388731754
100-101	0.1406596246991304
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2429624.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.174547066218985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.1223472857983	39.30485831135132
2	8.83184317996843	8.15612717189835
3	2.3701241047979034	3.283182210893128
4	1.0300882647484502	1.902554362519486
5	0.5406546121696625	1.2482240918098226
6	0.34782415695186564	0.9636373743565116
7	0.24073247823268099	0.7780999202565727
8	0.16758143663809433	0.6190397546776224
9	0.11690145689281382	0.48580846410661216
>10	0.9112910467677735	8.436624225723396
>50	0.14677532466041654	4.813352248889402
>100	0.14732978523272344	14.068748521925
>500	0.016029490602194146	5.177153083704416
>1k	0.009850524951069027	8.48138456742168
>5k	5.373013609674015E-4	1.806018251962444
>10k+	8.955022682790025E-5	0.4751874385043021
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	11492	0.47299499840304504	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	9173	0.37754813090420575	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	8549	0.3518651445655789	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	7203	0.2964656259569382	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	6895	0.28378876731543645	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	6791	0.279508269592332	No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC	5066	0.20850962947353172	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	4959	0.20410565585456844	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	4958	0.20406449722261555	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	4630	0.19056446594205523	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	4296	0.1768174828697774	No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC	3914	0.161094885463759	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	3871	0.1593250642897831	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	3804	0.15656743594893693	No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA	3685	0.15166955874653856	No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG	3665	0.15084638610748	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	3451	0.14203843886955347	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	3420	0.1407625212790127	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	2992	0.12314662680315966	No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA	2919	0.12014204667059594	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	2870	0.1181252737049025	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	2772	0.11409172777351557	No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA	2683	0.11042860952970501	No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT	2578	0.1061069531746476	No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT	2547	0.10483103558410684	No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC	2478	0.10199108997935483	No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG	2469	0.10162066229177848	No Hit
GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG	2437	0.1003035860692848	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	4.115863195292769E-5	0.0
9	0.0	0.0	0.0	4.115863195292769E-5	0.0
10-11	0.0	0.0	0.0	4.115863195292769E-5	0.0
12-13	0.0	0.0	0.0	4.115863195292769E-5	0.0
14-15	0.0	0.0	0.0	4.115863195292769E-5	4.115863195292769E-5
16-17	0.0	0.0	0.0	4.115863195292769E-5	8.231726390585538E-5
18-19	0.0	0.0	0.0	4.115863195292769E-5	8.231726390585538E-5
20-21	0.0	0.0	0.0	4.115863195292769E-5	8.231726390585538E-5
22-23	0.0	0.0	0.0	6.173794792939154E-5	8.231726390585538E-5
24-25	0.0	0.0	0.0	1.6463452781171077E-4	8.231726390585538E-5
26-27	0.0	0.0	0.0	4.5274495148220464E-4	8.231726390585538E-5
28-29	0.0	2.0579315976463846E-5	0.0	0.0012964969065172222	8.231726390585538E-5
30-31	0.0	4.115863195292769E-5	0.0	0.006029739581103907	1.0289657988231923E-4
32-33	0.0	4.115863195292769E-5	0.0	0.019056446594205523	1.2347589585878308E-4
34-35	0.0	4.115863195292769E-5	0.0	0.04050009384168085	1.2347589585878308E-4
36-37	0.0	4.115863195292769E-5	0.0	0.07408553751526985	1.2347589585878308E-4
38-39	0.0	4.115863195292769E-5	0.0	0.12434022712979456	1.2347589585878308E-4
40-41	0.0	4.115863195292769E-5	0.0	0.1806863942733526	1.2347589585878308E-4
42-43	0.0	4.115863195292769E-5	0.0	0.2400371415494743	1.2347589585878308E-4
44-45	0.0	8.231726390585538E-5	0.0	0.3146783205961087	1.2347589585878308E-4
46-47	0.0	1.2347589585878308E-4	0.0	0.40351922766650317	1.2347589585878308E-4
48-49	0.0	1.2347589585878308E-4	0.0	0.4959409357168023	1.2347589585878308E-4
50-51	0.0	1.6463452781171077E-4	0.0	0.5937338452369585	1.2347589585878308E-4
52-53	0.0	1.6463452781171077E-4	0.0	0.6974741770743127	1.440552118352469E-4
54-55	0.0	1.6463452781171077E-4	0.0	0.8123890774868869	1.6463452781171077E-4
56-57	0.0	2.057931597646385E-4	0.0	0.9480273490877601	1.6463452781171077E-4
58-59	0.0	2.057931597646385E-4	0.0	1.1065703993704377	2.2637247574110232E-4
60-61	0.0	2.057931597646385E-4	0.0	1.2717811480294894	2.881104236704939E-4
62-63	0.0	2.057931597646385E-4	0.0	1.4403874838246575	2.881104236704939E-4
64-65	0.0	2.057931597646385E-4	0.0	1.6198802777713754	2.881104236704939E-4
66-67	0.0	2.057931597646385E-4	0.0	1.829048445356154	2.881104236704939E-4
68-69	0.0	2.057931597646385E-4	0.0	2.0541450035067155	2.881104236704939E-4
70-71	0.0	2.2637247574110232E-4	0.0	2.306612051905974	2.881104236704939E-4
72-73	0.0	2.4695179171756615E-4	0.0	2.5802963750769665	2.881104236704939E-4
74-75	0.0	2.4695179171756615E-4	0.0	2.842785550356763	2.881104236704939E-4
76-77	0.0	2.6753110769403E-4	0.0	3.1405888318521713	2.881104236704939E-4
78-79	0.0	2.881104236704939E-4	0.0	3.4616878990329365	2.881104236704939E-4
80-81	0.0	2.881104236704939E-4	0.0	3.832156745241239	2.881104236704939E-4
82-83	0.0	2.881104236704939E-4	0.0	4.2267857084059095	2.881104236704939E-4
84-85	0.0	2.881104236704939E-4	0.0	4.614685235246277	2.881104236704939E-4
86-87	0.0	2.881104236704939E-4	0.0	5.027403417154259	3.0868973964695773E-4
88-89	0.0	2.881104236704939E-4	0.0	5.478522602674323	3.2926905562342154E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	2390	0.0	47.36683	2
GTACCTG	2605	0.0	44.366627	1
ACCTGGG	2560	0.0	43.29535	3
CCTGGGG	2470	0.0	33.75057	4
CTGGGGG	2225	0.0	32.783558	5
GTACACG	295	0.0	32.113102	1
TGGGGGG	4995	0.0	29.491053	6
CATGGGG	4695	0.0	27.844463	4
GTACTAG	1175	0.0	27.412289	1
AGGGCTA	3850	0.0	26.328032	5
GCTATTG	3900	0.0	25.990494	8
CTATTGA	4015	0.0	25.482527	9
ATGGGGG	4075	0.0	25.455511	5
GGGGGGA	4965	0.0	25.185469	7
GTACATG	8065	0.0	25.019552	1
ACATGGG	7955	0.0	24.71003	3
TAGGCAT	1665	0.0	23.896286	5
TACATGG	8345	0.0	23.896267	2
GGGGAAT	5270	0.0	23.458714	9
GGGCTAT	4465	0.0	23.44424	6
>>END_MODULE