FastQCFastQC Report
Sat 18 Jun 2016
SRR1931056_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931056_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2429624
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA123320.5075682492435043No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC95370.3925298729350714No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA89140.36688804522839746No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC75060.3089366914386753No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC74880.3081958360635226No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC66250.272675936688146No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC53360.2196224601008222No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG49870.2052580975492504No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC49270.20278857963207478No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA45480.18718945812191518No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA43480.17895773173132962No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC41920.1725369851466729No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC41210.16961472227801502No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA39990.16459336917975784No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC37880.1559088978376901No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG37240.15327474539270275No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC36600.15064059294771537No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36470.1501055307323273No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT34230.14088599717487152No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT33340.13722287893106094No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA28370.11676703885045588No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA28040.11540880399600927No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT27620.11368014145398629No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT27320.11244538249539845No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT26930.11084019584923427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26440.10882342288354083No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT26220.10791793298057643No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC25720.10586000138293003No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA25540.10511914600777733No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA24970.10277310398646045No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA24520.1009209655485787No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG24360.10026242743733187No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG23500.051.5415272
ACCTGGG24800.048.8397523
CCTGGGG20500.046.1088374
CTGGGGG17450.044.368745
GTACCTG27400.044.2417451
TATCACG1850.038.5127452
TGGGGGG45900.031.7695186
GTGATCG7800.026.7943388
CTGTGCG15250.026.7863529
GGGGGGA47700.025.8904697
CATGGGG50050.025.7187674
ATGGGGG41550.025.2642365
GCTATTG35200.025.0989328
AGGGCTA35400.025.0913095
TAGTACT17050.024.7942284
TCACGCA2501.4551915E-1124.6995074
ATAACGC2900.024.5684763
TAACGCC5650.024.3800454
TAACGCA2751.8189894E-1224.1813354
GTACATG90300.024.1112821
Produced by FastQC (version 0.11.5)