##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931055_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3973187 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.228698775063947 33.0 33.0 33.0 27.0 33.0 2 31.148548256097687 33.0 33.0 33.0 27.0 33.0 3 31.173614028234766 33.0 33.0 33.0 27.0 33.0 4 34.803327404423705 37.0 37.0 37.0 33.0 37.0 5 34.7862891930332 37.0 37.0 37.0 33.0 37.0 6 34.87953071426037 37.0 37.0 37.0 33.0 37.0 7 34.84623250805965 37.0 37.0 37.0 33.0 37.0 8 34.846586631839884 37.0 37.0 37.0 33.0 37.0 9 34.86714040894627 37.0 37.0 37.0 33.0 37.0 10-11 34.770636267560526 37.0 37.0 37.0 33.0 37.0 12-13 34.69121740305704 37.0 37.0 37.0 33.0 37.0 14-15 36.362257552941756 40.0 37.0 40.0 33.0 40.0 16-17 36.3105670586358 40.0 37.0 40.0 33.0 40.0 18-19 36.248829843649446 40.0 37.0 40.0 33.0 40.0 20-21 36.16521850091627 40.0 37.0 40.0 33.0 40.0 22-23 36.077023054792036 40.0 37.0 40.0 33.0 40.0 24-25 35.95904672999282 40.0 37.0 40.0 33.0 40.0 26-27 35.79153007396833 40.0 37.0 40.0 27.0 40.0 28-29 35.678573145437156 40.0 37.0 40.0 27.0 40.0 30-31 35.547658340772784 40.0 37.0 40.0 27.0 40.0 32-33 35.396967598051646 37.0 37.0 40.0 27.0 40.0 34-35 35.28873093564435 37.0 37.0 40.0 27.0 40.0 36-37 35.086817584976494 37.0 37.0 40.0 27.0 40.0 38-39 34.91063773237957 37.0 37.0 40.0 27.0 40.0 40-41 34.69210233497694 37.0 37.0 40.0 24.5 40.0 42-43 34.52242997875509 37.0 37.0 40.0 22.0 40.0 44-45 34.27748228311428 37.0 33.0 40.0 22.0 40.0 46-47 34.13698851828519 37.0 33.0 40.0 22.0 40.0 48-49 34.14052623246779 37.0 33.0 40.0 22.0 40.0 50-51 33.35031084617965 37.0 33.0 38.5 22.0 40.0 52-53 33.46745219895263 37.0 33.0 38.5 22.0 40.0 54-55 33.981852477620606 37.0 33.0 40.0 22.0 40.0 56-57 33.94636044062361 37.0 33.0 40.0 22.0 40.0 58-59 33.80279999406018 37.0 33.0 40.0 22.0 40.0 60-61 33.824183709450374 37.0 33.0 40.0 22.0 40.0 62-63 33.66543709118146 37.0 33.0 40.0 18.5 40.0 64-65 33.45562617616538 37.0 33.0 40.0 15.0 40.0 66-67 33.226987554323514 37.0 33.0 40.0 15.0 40.0 68-69 33.00397590146147 37.0 33.0 40.0 15.0 40.0 70-71 32.768458167209346 37.0 33.0 40.0 15.0 40.0 72-73 32.164136372136525 37.0 33.0 37.0 6.0 40.0 74-75 31.979754287930568 37.0 33.0 37.0 6.0 40.0 76-77 31.612300402674226 37.0 33.0 37.0 2.0 40.0 78-79 31.346008758208463 37.0 33.0 37.0 2.0 37.0 80-81 31.083855730928345 37.0 33.0 37.0 2.0 37.0 82-83 30.8135422521014 37.0 33.0 37.0 2.0 37.0 84-85 30.42973877141952 37.0 33.0 37.0 2.0 37.0 86-87 30.256647497336523 37.0 33.0 37.0 2.0 37.0 88-89 30.055678854279954 35.0 33.0 37.0 2.0 37.0 90-91 29.777181642847417 33.0 33.0 37.0 2.0 37.0 92-93 29.49965179086713 33.0 27.0 37.0 2.0 37.0 94-95 29.253512860079326 33.0 27.0 37.0 2.0 37.0 96-97 28.956607755940006 33.0 27.0 37.0 2.0 37.0 98-99 28.630306728578343 33.0 27.0 37.0 2.0 37.0 100-101 27.92927415699286 33.0 27.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06054178460741966 1101 2 0.10987862065857712 1101 3 0.15816126139802833 1101 4 0.16745638958008868 1101 5 0.0407420865139656 1101 6 0.11926725409169592 1101 7 0.06915215579959977 1101 8 0.0862384509883185 1101 9 0.0853486534018515 1101 10-11 0.1370320969138561 1101 12-13 0.21430913394927842 1101 14-15 0.36456436157110517 1101 16-17 0.28467723967288805 1101 18-19 0.27380688563586375 1101 20-21 0.3450012868079355 1101 22-23 0.3833802644619908 1101 24-25 0.3139502604749822 1101 26-27 0.4324151582476006 1101 28-29 0.35502870106574136 1101 30-31 0.312146464894532 1101 32-33 0.35345336419766227 1101 34-35 0.4106309958100596 1101 36-37 0.3442198836454011 1101 38-39 0.23999047645378369 1101 40-41 0.35010553623778407 1101 42-43 0.24161805008860426 1101 44-45 0.3093791509835242 1101 46-47 0.24798263833597645 1101 48-49 0.2892544727420727 1101 50-51 0.2965572179882372 1101 52-53 0.3358518185811121 1101 54-55 0.4988900562438445 1101 56-57 0.5008071449537965 1101 58-59 0.6239902959739894 1101 60-61 0.5044154824150979 1101 62-63 0.49988332509774125 1101 64-65 0.5505274715151529 1101 66-67 0.5355831455119784 1101 68-69 0.568766555774296 1101 70-71 0.46308626507472184 1101 72-73 0.464489816806946 1101 74-75 0.5347814826603212 1101 76-77 0.3355302981513084 1101 78-79 0.31922203532807103 1101 80-81 0.48887331226597297 1101 82-83 0.4092190589593976 1101 84-85 0.37677012862945247 1101 86-87 0.4177899050105012 1101 88-89 0.3413177177364659 1101 90-91 0.3648131182040615 1101 92-93 0.3399623352360237 1101 94-95 0.32168860434120106 1101 96-97 0.4054604180957071 1101 98-99 0.49250369820039097 1101 100-101 0.5001725955605778 1102 1 0.14054309122268194 1102 2 0.07750706977569521 1102 3 0.0786474106450541 1102 4 0.10534821635446434 1102 5 -0.008185920878823083 1102 6 0.04808031405839586 1102 7 0.17032260218229567 1102 8 0.14453810899597386 1102 9 0.16338498740553575 1102 10-11 0.12681098857588324 1102 12-13 0.10605629632178903 1102 14-15 0.23189127134394738 1102 16-17 0.20577677219498014 1102 18-19 0.17333215664805834 1102 20-21 0.20821042541393808 1102 22-23 0.1367849203677025 1102 24-25 0.1271559984096271 1102 26-27 0.14801689402601426 1102 28-29 0.1892709841455158 1102 30-31 0.07803612155139916 1102 32-33 0.2310725123602566 1102 34-35 0.11392498392806516 1102 36-37 0.09082848325682136 1102 38-39 0.0015616749923950124 1102 40-41 -0.014520285762081642 1102 42-43 0.05044473325523313 1102 44-45 0.06222305117326954 1102 46-47 -0.06942158739663995 1102 48-49 -0.007842024582203067 1102 50-51 -0.08253806614061432 1102 52-53 0.0672398889944148 1102 54-55 0.07322115760057812 1102 56-57 0.06956386739462062 1102 58-59 0.019175805358266018 1102 60-61 0.10159870080416056 1102 62-63 0.12116282493551722 1102 64-65 0.06873944743303895 1102 66-67 0.14198305120844168 1102 68-69 0.05388715888769724 1102 70-71 0.1228405346136654 1102 72-73 -0.11932450243387649 1102 74-75 -0.07970440684169233 1102 76-77 -0.16714096470812123 1102 78-79 -0.06129557367962235 1102 80-81 -0.030501006810737863 1102 82-83 -0.07955915951172088 1102 84-85 -0.08052970832768835 1102 86-87 -0.1350723872092665 1102 88-89 -0.10236740124127053 1102 90-91 -0.06332035980377171 1102 92-93 -0.017174080131287184 1102 94-95 -0.15547486174928693 1102 96-97 -0.14314180340272387 1102 98-99 -0.23895135991558547 1102 100-101 -0.1269471036527996 1103 1 0.13562804616425694 1103 2 0.11140601546125595 1103 3 0.10937310110390541 1103 4 0.09524582045041541 1103 5 0.08512992437173494 1103 6 0.10509645866675044 1103 7 0.12028455377257785 1103 8 0.10057114043196691 1103 9 0.0948306662698144 1103 10-11 0.03132421147707021 1103 12-13 0.20125101430376446 1103 14-15 0.27130403721781704 1103 16-17 0.23032201409031217 1103 18-19 0.19055787850392392 1103 20-21 0.28799646746568897 1103 22-23 0.18159964529570516 1103 24-25 0.2159166737636795 1103 26-27 0.25077483084690755 1103 28-29 0.22628578508106045 1103 30-31 0.2394013690237884 1103 32-33 0.2130949363698349 1103 34-35 0.28901064865029724 1103 36-37 0.2487464957043244 1103 38-39 0.21184702913723186 1103 40-41 0.19152189693397048 1103 42-43 0.15950967387024662 1103 44-45 0.35253435174439574 1103 46-47 0.10716648358619096 1103 48-49 0.013220716456771697 1103 50-51 0.14160237013790322 1103 52-53 0.32784958462796254 1103 54-55 0.36488543105154037 1103 56-57 0.4107039075649368 1103 58-59 0.4414796730230037 1103 60-61 0.3853631676071103 1103 62-63 0.32246852374507995 1103 64-65 0.3310722955759857 1103 66-67 0.27574140229125277 1103 68-69 0.29401213087591316 1103 70-71 0.30818855628979946 1103 72-73 0.24686677735272866 1103 74-75 0.2706206712118586 1103 76-77 0.26297551223234095 1103 78-79 0.22198424834250474 1103 80-81 0.33237878164189283 1103 82-83 0.3450059621940831 1103 84-85 0.3106155324367563 1103 86-87 0.22511789799161264 1103 88-89 0.23495150425017286 1103 90-91 0.2522909384162979 1103 92-93 0.1368103784995114 1103 94-95 0.19076503429239722 1103 96-97 0.1921163573636413 1103 98-99 0.24831529411292053 1103 100-101 0.3257132048473359 1104 1 0.19184202088842284 1104 2 0.2630034103259753 1104 3 0.18655129154422667 1104 4 0.2223153383244636 1104 5 0.1653085799551306 1104 6 0.24908962710394178 1104 7 0.23989772508803497 1104 8 0.254697594212864 1104 9 0.23229763512638613 1104 10-11 0.22976131959541846 1104 12-13 0.18894855745197958 1104 14-15 0.32901184218507495 1104 16-17 0.35535120731620395 1104 18-19 0.37364324687302286 1104 20-21 0.41080328722595283 1104 22-23 0.42077396900801034 1104 24-25 0.42564428809187405 1104 26-27 0.44401965059770276 1104 28-29 0.3499435230048036 1104 30-31 0.3068037954505982 1104 32-33 0.4462363877442854 1104 34-35 0.39655365629162276 1104 36-37 0.42499856149910187 1104 38-39 0.40152613469297904 1104 40-41 0.4950587936919675 1104 42-43 0.37612807254770075 1104 44-45 0.4216204861660131 1104 46-47 0.3238434078964687 1104 48-49 0.22360690073304568 1104 50-51 0.22972071328210575 1104 52-53 0.19592108221331728 1104 54-55 0.4193723776781084 1104 56-57 0.5459167348804996 1104 58-59 0.4068926245994149 1104 60-61 0.33498947343854724 1104 62-63 0.36361447929677837 1104 64-65 0.32149121106051126 1104 66-67 0.4257543252520364 1104 68-69 0.4600815020531286 1104 70-71 0.43427479481884745 1104 72-73 0.42890331210080745 1104 74-75 0.3729603255919045 1104 76-77 0.35363495345381324 1104 78-79 0.4666629739339747 1104 80-81 0.46585595163456617 1104 82-83 0.37984611136277024 1104 84-85 0.4675772434506804 1104 86-87 0.4636541461659043 1104 88-89 0.3294286121680976 1104 90-91 0.4720185374345718 1104 92-93 0.4802387919774702 1104 94-95 0.47829329152570565 1104 96-97 0.4331377528318505 1104 98-99 0.5031456421518463 1104 100-101 0.5196544865879211 1105 1 0.011235541659203818 1105 2 -0.029560951469775887 1105 3 0.0330709870331809 1105 4 0.05285942856707493 1105 5 -0.053898904162103634 1105 6 -0.005789996583771995 1105 7 0.031723336992079965 1105 8 -0.007357318287709802 1105 9 -0.022516522266869288 1105 10-11 -0.034836161350426664 1105 12-13 -0.032803095833301654 1105 14-15 0.08954084120544081 1105 16-17 0.0060550745186773725 1105 18-19 0.1115258081810282 1105 20-21 0.15748427023437017 1105 22-23 0.09706163287138736 1105 24-25 0.007816117383477206 1105 26-27 0.13940713853837394 1105 28-29 0.08523294609517507 1105 30-31 0.008223394804709017 1105 32-33 0.009413289301669181 1105 34-35 0.17156263013946926 1105 36-37 0.11404512920321253 1105 38-39 0.09168945153690089 1105 40-41 0.08226639881078057 1105 42-43 -0.02758724420006331 1105 44-45 0.040149629708771783 1105 46-47 0.009342643273676288 1105 48-49 0.1077703362264657 1105 50-51 0.09516340198412365 1105 52-53 0.0669084785795846 1105 54-55 0.19681213071990555 1105 56-57 0.06126753556202402 1105 58-59 0.133619585592605 1105 60-61 0.08736680809270325 1105 62-63 0.10290476194260378 1105 64-65 0.17631888639422044 1105 66-67 0.15276085737696832 1105 68-69 0.12977948029453756 1105 70-71 0.22377115908742695 1105 72-73 0.09942432318786842 1105 74-75 0.08911114791258967 1105 76-77 0.12959132650611238 1105 78-79 0.19056289517841662 1105 80-81 0.225725485541993 1105 82-83 0.28272268204748485 1105 84-85 0.273966590451181 1105 86-87 0.28016202113390776 1105 88-89 0.12199643675188199 1105 90-91 0.15900458292149366 1105 92-93 0.27015941311540104 1105 94-95 0.20059585478594144 1105 96-97 0.089913586177925 1105 98-99 0.135639327790539 1105 100-101 0.15793743112856262 1106 1 -0.022508593511574304 1106 2 0.009396343475117419 1106 3 0.07843571763448765 1106 4 -0.004294550261775498 1106 5 0.006978555091343708 1106 6 0.01100975393539727 1106 7 0.1364522204413774 1106 8 0.01921920354597262 1106 9 0.08982595151721284 1106 10-11 0.04308551012933037 1106 12-13 0.051610696675979284 1106 14-15 0.09446897514186503 1106 16-17 0.14396091326023708 1106 18-19 -0.007183308690528634 1106 20-21 0.05431464194017366 1106 22-23 0.07921752731927256 1106 24-25 0.10473860850016337 1106 26-27 0.1905580829222444 1106 28-29 0.20726267598048764 1106 30-31 0.24645330276754152 1106 32-33 0.18478455715689535 1106 34-35 0.12493812710717833 1106 36-37 0.24190558572231424 1106 38-39 0.1133780445901067 1106 40-41 0.27328621825569144 1106 42-43 0.18631250711538883 1106 44-45 0.2689568458554774 1106 46-47 0.16652773541070331 1106 48-49 0.17101542881515286 1106 50-51 0.2514295843962202 1106 52-53 0.3425651116666799 1106 54-55 0.3700891080858426 1106 56-57 0.3751286938438483 1106 58-59 0.41903572657903254 1106 60-61 0.4294681070716919 1106 62-63 0.4433662649183745 1106 64-65 0.41015027850165353 1106 66-67 0.4414428840666673 1106 68-69 0.5432546560256597 1106 70-71 0.5328403653449669 1106 72-73 0.386804381613274 1106 74-75 0.47409300794669207 1106 76-77 0.28853816811045974 1106 78-79 0.35418609898370335 1106 80-81 0.42907712701538614 1106 82-83 0.4425065871664522 1106 84-85 0.43884359465535994 1106 86-87 0.5653016537710407 1106 88-89 0.5719687565463012 1106 90-91 0.4548036401628046 1106 92-93 0.4559500494651161 1106 94-95 0.6254143017469254 1106 96-97 0.5667917054224993 1106 98-99 0.6459776332920271 1106 100-101 0.6590562244412972 1107 1 0.15021574264067183 1107 2 0.16664747241734545 1107 3 0.1260512683847601 1107 4 0.18050622219653434 1107 5 0.15613574415086617 1107 6 0.21331847412076854 1107 7 0.20786134053739858 1107 8 0.227084011102356 1107 9 0.2701795811140144 1107 10-11 0.2335710984403434 1107 12-13 0.2187157832650115 1107 14-15 0.3453782702998822 1107 16-17 0.3129416328371448 1107 18-19 0.28943421385662305 1107 20-21 0.3670374307279971 1107 22-23 0.3232065191763809 1107 24-25 0.3081685122357385 1107 26-27 0.33751574152382346 1107 28-29 0.18413853845038375 1107 30-31 0.3991095461426113 1107 32-33 0.32582245403511223 1107 34-35 0.30292284666485614 1107 36-37 0.36986890881419043 1107 38-39 0.3855882973219238 1107 40-41 0.41075599766009674 1107 42-43 0.4472221913000567 1107 44-45 0.4099298321071032 1107 46-47 0.47349161843869325 1107 48-49 0.4709462345243196 1107 50-51 0.47207391743626204 1107 52-53 0.45691578028984026 1107 54-55 0.5623869486382418 1107 56-57 0.5959205510352348 1107 58-59 0.5572193043216416 1107 60-61 0.49387474745064 1107 62-63 0.5335548816488753 1107 64-65 0.44747607433435377 1107 66-67 0.4726609614794697 1107 68-69 0.4374857404789836 1107 70-71 0.5169903705247023 1107 72-73 0.43739139660304005 1107 74-75 0.5512807036893861 1107 76-77 0.5227667435604246 1107 78-79 0.5778875695481567 1107 80-81 0.5603613826697114 1107 82-83 0.6709900257773889 1107 84-85 0.7418975412870239 1107 86-87 0.5942998751761621 1107 88-89 0.638147653730055 1107 90-91 0.5680123625774236 1107 92-93 0.7011799926278854 1107 94-95 0.7524935727022353 1107 96-97 0.7594068868160129 1107 98-99 0.7785089597723704 1107 100-101 0.748051534703162 1108 1 -0.13482773577890583 1108 2 -0.09680703058123896 1108 3 -0.09814717650129623 1108 4 -0.13591684691252937 1108 5 -0.16133371817549857 1108 6 -0.19081522967660192 1108 7 -0.14910242172361166 1108 8 -0.040675898633821816 1108 9 -0.07376808987112327 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2316 70-71 -0.6817089765534448 2316 72-73 -0.33986096206792027 2316 74-75 -0.466421997519042 2316 76-77 -0.5279933313633549 2316 78-79 -0.6176040320059393 2316 80-81 -0.5399826472929234 2316 82-83 -0.41378608546769513 2316 84-85 -0.47721061974629464 2316 86-87 -0.5364595844159972 2316 88-89 -0.47613784856990193 2316 90-91 -0.4820512245549935 2316 92-93 -0.44555204929968895 2316 94-95 -0.4143405340639852 2316 96-97 -0.4284522822238941 2316 98-99 -0.41924541783372504 2316 100-101 -0.5700769198410462 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 107128.0 3 16410.0 4 12181.0 5 10633.0 6 10739.0 7 11276.0 8 11642.0 9 12097.0 10 12626.0 11 12624.0 12 13414.0 13 14409.0 14 14958.0 15 16767.0 16 19588.0 17 23820.0 18 20793.0 19 16707.0 20 17256.0 21 18047.0 22 20306.0 23 22469.0 24 26748.0 25 30436.0 26 34158.0 27 40906.0 28 48322.0 29 59039.0 30 72424.0 31 90922.0 32 117435.0 33 156385.0 34 216755.0 35 314348.0 36 492601.0 37 899763.0 38 936381.0 39 674.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.80070593523708 22.85367171645606 12.976920941444977 23.368701406861877 2 14.228514516455022 21.69004966721981 43.14375656140646 20.937679254918713 3 17.364021422633403 30.41754201482389 30.13962867536113 22.078807887181583 4 11.127633109615182 18.342782585321384 42.98313128562346 27.54645301943997 5 11.866226641712231 38.75978201448337 36.677054839687216 12.69693650411718 6 25.81831663095646 41.192171423091835 20.794339657307848 12.195172288643851 7 23.99652973796602 32.66133710796899 27.12902765462587 16.213105499439116 8 23.02484126722452 37.31513266302341 22.331720102778956 17.32830596697311 9 25.660231949817614 15.84886389691701 23.58851974498054 34.90238440828484 10-11 22.9060661778817 27.493216714900427 30.72284191110634 18.877875196111532 12-13 23.54099086010227 25.355605811912334 30.290387974683785 20.813015353301616 14-15 21.082805946467296 26.909176531469036 27.69020157823414 24.317815943829522 16-17 19.922187361959022 29.10543782351142 29.34531922310694 21.627055591422614 18-19 19.622517560578725 29.736177592305125 30.553550719758775 20.08775412735738 20-21 20.487286525221823 28.54459871750278 29.81376910116969 21.154345656105704 22-23 20.63611654825207 29.106143254772554 29.106659213372037 21.15108098360334 24-25 20.46862392045074 28.949223620540295 29.869618487531163 20.7125339714778 26-27 20.64336513735699 28.692772326094897 29.29659489976183 21.367267636786288 28-29 20.281300628437574 29.621246118040755 29.033204326904322 21.06424892661735 30-31 21.334334376912036 29.009432478259896 28.826921561960212 20.82931158286786 32-33 21.15182346061235 29.24363992935646 28.70301599194803 20.901520618083165 34-35 21.386144170913678 28.444621408456232 29.447934366039153 20.721300054590934 36-37 21.52798244834688 28.754548930115796 29.274534523544954 20.442934097992367 38-39 21.90027803876334 28.316814688057722 29.782615316117766 20.00029195706117 40-41 21.14650316358924 28.31672236097209 29.895773583708955 20.641000891729718 42-43 21.52870517514156 28.373967390401557 29.093427793211795 21.003899641245084 44-45 21.02852441307393 29.335002534507815 29.04954950193272 20.586923550485533 46-47 21.62862151218237 28.572028082321864 28.47640700044704 21.322943405048722 48-49 21.78541958870962 27.83047119674312 29.168135331949564 21.2159738825977 50-51 21.630066593183894 28.343656765783326 28.310878883196633 21.715397757836143 52-53 20.714216532393966 28.709220499393677 28.84894627319855 21.727616695013804 54-55 21.202054751711188 28.965569663535227 28.358040851845928 21.474334732907657 56-57 21.612094826078053 28.19430374042817 28.72402543552749 21.469575997966288 58-59 21.24873476910664 27.918399660105877 29.62482519649498 21.2080403742925 60-61 21.22063132708922 29.39254895725279 28.236289571518352 21.150530144139644 62-63 21.621046959811377 28.667788467908473 27.747797881448697 21.96336669083145 64-65 20.566171156074734 28.66695419471285 28.323081419059427 22.443793230152995 66-67 21.27480550625905 27.523422393395276 28.895230635725984 22.306541464619688 68-69 20.5074025373785 28.35128675283366 28.393544438434464 22.747766271353377 70-71 20.72309807113628 28.099539622119735 28.89344293317683 22.283919373567155 72-73 21.441335706863146 27.88682678265327 29.09599473188037 21.575842778603214 74-75 21.422789049971644 28.718822287640567 28.13301798914431 21.72537067324348 76-77 21.006279574424646 28.46916027568221 28.970378778072988 21.55418137182016 78-79 20.813490823095552 28.3661542250386 28.650355678061622 22.169999273804226 80-81 20.77387403831904 28.647507904131963 29.242182906271623 21.33643515127737 82-83 21.582318140943727 27.589581459920804 29.502995882436462 21.32510451669901 84-85 22.227957232416447 27.77964621325059 28.4334794414818 21.558917112851162 86-87 21.161721916016177 28.67497823993464 28.600213695890485 21.5630861481587 88-89 21.823542600076486 27.63454506755247 28.764875415614576 21.77703691675647 90-91 21.71424735497404 27.30086469964154 29.250451479003832 21.734436466380586 92-93 21.473462705087115 28.021455704033578 28.793238991231785 21.711842599647525 94-95 20.6945316360382 27.74294807208406 29.185706095563447 22.376814196314296 96-97 21.14712722889586 27.757735993065253 28.534227718981352 22.560909059057533 98-99 20.69660534184172 28.424725649113707 28.642098923872467 22.236570085172108 100-101 21.300742919764794 27.311795024977204 28.395453373891748 22.992008681366258 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 89.0 1 63.0 2 63.0 3 91.0 4 70.5 5 57.5 6 98.5 7 170.0 8 278.0 9 339.0 10 315.5 11 327.5 12 428.0 13 620.0 14 819.0 15 1041.5 16 1300.0 17 1528.5 18 1756.0 19 2008.0 20 2281.0 21 2603.5 22 3035.0 23 3561.5 24 4093.0 25 4861.5 26 6306.5 27 8614.5 28 13990.5 29 26671.0 30 45752.0 31 68275.0 32 96144.5 33 131152.5 34 174585.0 35 207412.0 36 229249.0 37 256211.0 38 254504.5 39 213755.5 40 173295.5 41 142855.5 42 122503.5 43 112294.0 44 105255.0 45 105504.5 46 108779.0 47 112209.5 48 117502.5 49 115125.5 50 107799.5 51 104711.5 52 99649.5 53 94640.5 54 90088.5 55 80839.0 56 71825.0 57 60765.5 58 49814.5 59 45199.5 60 38908.0 61 30579.0 62 24214.0 63 20003.0 64 15864.5 65 11227.5 66 8477.5 67 6719.0 68 5427.0 69 4469.0 70 3697.5 71 3108.0 72 2609.0 73 2190.0 74 1872.0 75 1638.5 76 1333.5 77 1077.0 78 910.5 79 732.5 80 575.5 81 492.5 82 397.5 83 307.0 84 254.0 85 209.5 86 157.0 87 134.5 88 122.5 89 90.5 90 65.0 91 46.5 92 38.0 93 29.0 94 24.0 95 16.5 96 11.5 97 12.0 98 7.0 99 2.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.530368190573462E-4 2 0.0036242945524587694 3 1.5101227301911538E-4 4 8.305675016051346E-4 5 0.001988328261418353 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.7753068254778846E-5 12-13 6.292178042463142E-5 14-15 0.003498450991609506 16-17 0.004580705614913166 18-19 0.0038885660302422215 20-21 2.5168712169852567E-5 22-23 0.0 24-25 2.5168712169852567E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 2.5168712169852566E-4 42-43 2.5168712169852567E-5 44-45 7.298926529257243E-4 46-47 0.7423763341619712 48-49 0.5332243360304965 50-51 0.4090041571161891 52-53 0.34343966191372316 54-55 0.3345551065177652 56-57 0.3009171227027573 58-59 0.3008542009223326 60-61 0.2782904504620598 62-63 0.2720611942000213 64-65 0.26867600241317613 66-67 0.2568718764055153 68-69 0.23686275023048248 70-71 0.24969879343710727 72-73 0.818033482944548 74-75 0.5417816981682464 76-77 0.6369697675946288 78-79 0.3573831284558215 80-81 0.30917246029446893 82-83 0.28942760559721953 84-85 0.2810715931568285 86-87 0.2536628656038591 88-89 0.25748850985367666 90-91 0.26817262816977905 92-93 0.2246685091841889 94-95 0.19184850851470117 96-97 0.1489987760455272 98-99 0.1580091750023344 100-101 0.15276149851492013 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3973187.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.74861306644194 #Duplication Level Percentage of deduplicated Percentage of total 1 82.31132270289845 36.010062079161415 2 10.385814788346556 9.087299851102081 3 3.119824904082653 4.094640376912839 4 1.3544831262062609 2.3702703277368937 5 0.6970710169197962 1.5247945099527684 6 0.4257284840745245 1.1175018432683557 7 0.2678591414163915 0.8202926153894544 8 0.18481698550139894 0.6468389429445528 9 0.1343254043365237 0.5288895125380744 >10 0.8308931309962112 7.074006317333689 >50 0.11934289672592648 3.6793748660487275 >100 0.13591203993570433 12.698543737683474 >500 0.018904182250807845 5.7192913097423315 >1k 0.012660599120889699 10.42138414353715 >5k 6.937314586788877E-4 2.3301050625871014 >10k+ 3.4686572933944384E-4 1.8767045040611168 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 15116 0.3804502531594913 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 14786 0.37214457814344004 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 11926 0.30016206133766166 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 11835 0.2978717085302051 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 10465 0.26339057285750705 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 10075 0.2535747751112646 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 9634 0.24247537304435962 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 9141 0.23006719794462227 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 9087 0.22870808748745025 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 8984 0.22611571013395546 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 8371 0.2106872895738358 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 7578 0.19072850082314274 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 7473 0.1880857860453082 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 7228 0.18191945156369435 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 7155 0.1800821355752951 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 6014 0.15136463498949332 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 5963 0.15008103066883083 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 5502 0.1384782543585288 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 4955 0.12471096880161946 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 4942 0.12438377554341137 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 4917 0.12375455773916506 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 4833 0.12164038591689745 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 4490 0.11300751764263801 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 4405 0.11086817710820053 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 4350 0.10948389793885865 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 4283 0.10779759422347854 No Hit GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT 4268 0.10742006354093074 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 4204 0.1058092659620602 No Hit TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG 4108 0.10339306959375433 No Hit TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG 4033 0.10150541618101538 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.5168712169852564E-5 0.0 3 0.0 0.0 0.0 2.5168712169852564E-5 0.0 4 0.0 0.0 0.0 2.5168712169852564E-5 0.0 5 0.0 0.0 0.0 2.5168712169852564E-5 0.0 6 0.0 0.0 0.0 2.5168712169852564E-5 0.0 7 0.0 0.0 0.0 2.5168712169852564E-5 0.0 8 0.0 0.0 0.0 2.5168712169852564E-5 0.0 9 0.0 0.0 0.0 2.5168712169852564E-5 0.0 10-11 0.0 0.0 0.0 2.5168712169852564E-5 0.0 12-13 0.0 0.0 0.0 5.033742433970513E-5 2.5168712169852564E-5 14-15 0.0 0.0 0.0 6.29217804246314E-5 2.5168712169852564E-5 16-17 0.0 0.0 0.0 7.550613650955769E-5 5.033742433970513E-5 18-19 0.0 0.0 0.0 7.550613650955769E-5 5.033742433970513E-5 20-21 0.0 0.0 0.0 8.809049259448398E-5 1.0067484867941025E-4 22-23 0.0 0.0 0.0 1.258435608492628E-4 1.0067484867941025E-4 24-25 0.0 0.0 0.0 2.5168712169852566E-4 1.2584356084926283E-4 26-27 0.0 0.0 0.0 7.550613650955769E-4 1.2584356084926283E-4 28-29 0.0 0.0 0.0 0.002038665685758058 1.384279169341891E-4 30-31 0.0 1.2584356084926282E-5 0.0 0.007840053840909075 1.5101227301911538E-4 32-33 1.2584356084926282E-5 2.5168712169852564E-5 0.0 0.028553903956697733 1.5101227301911538E-4 34-35 2.5168712169852564E-5 2.5168712169852564E-5 0.0 0.06100895829972261 1.5101227301911538E-4 36-37 2.5168712169852564E-5 2.5168712169852564E-5 0.0 0.11435404374372513 1.5101227301911538E-4 38-39 2.5168712169852564E-5 2.5168712169852564E-5 0.0 0.18692802528549501 1.5101227301911538E-4 40-41 2.5168712169852564E-5 2.5168712169852564E-5 0.0 0.26556766646019936 1.5101227301911538E-4 42-43 2.5168712169852564E-5 2.5168712169852564E-5 0.0 0.3523997234461907 1.5101227301911538E-4 44-45 2.5168712169852564E-5 2.5168712169852564E-5 0.0 0.4544966043631976 1.6359662910404166E-4 46-47 2.5168712169852564E-5 2.5168712169852564E-5 0.0 0.5796354412717045 1.7618098518896796E-4 48-49 2.5168712169852564E-5 2.5168712169852564E-5 0.0 0.7179375146450444 2.013496973588205E-4 50-51 2.5168712169852564E-5 2.5168712169852564E-5 0.0 0.8553964361606943 2.013496973588205E-4 52-53 2.5168712169852564E-5 2.5168712169852564E-5 0.0 0.9960140310536605 2.265184095286731E-4 54-55 2.5168712169852564E-5 2.5168712169852564E-5 0.0 1.1547279299967506 2.265184095286731E-4 56-57 2.5168712169852564E-5 2.5168712169852564E-5 0.0 1.3454564308198935 2.265184095286731E-4 58-59 2.5168712169852564E-5 2.5168712169852564E-5 0.0 1.5683631301521928 2.265184095286731E-4 60-61 2.5168712169852564E-5 2.5168712169852564E-5 0.0 1.7983548219603054 2.265184095286731E-4 62-63 2.5168712169852564E-5 2.5168712169852564E-5 0.0 2.02806965793455 2.265184095286731E-4 64-65 2.5168712169852564E-5 2.5168712169852564E-5 0.0 2.2717279604508924 2.265184095286731E-4 66-67 2.5168712169852564E-5 2.5168712169852564E-5 0.0 2.5458781577610115 2.265184095286731E-4 68-69 2.5168712169852564E-5 2.5168712169852564E-5 0.0 2.84830540319396 2.265184095286731E-4 70-71 2.5168712169852564E-5 2.5168712169852564E-5 0.0 3.173824942042748 2.3910276561359936E-4 72-73 2.5168712169852564E-5 2.5168712169852564E-5 0.0 3.5318624570149857 2.5168712169852566E-4 74-75 2.5168712169852564E-5 2.5168712169852564E-5 0.0 3.881330025493389 2.5168712169852566E-4 76-77 2.5168712169852564E-5 2.5168712169852564E-5 0.0 4.271268883140914 2.6427147778345197E-4 78-79 2.5168712169852564E-5 2.5168712169852564E-5 0.0 4.698444850443737 2.768558338683782E-4 80-81 2.5168712169852564E-5 2.5168712169852564E-5 0.0 5.185761455476422 2.768558338683782E-4 82-83 2.5168712169852564E-5 2.5168712169852564E-5 0.0 5.710818544407802 3.0202454603823076E-4 84-85 2.5168712169852564E-5 2.5168712169852564E-5 0.0 6.219641813989626 3.271932582080833E-4 86-87 2.5168712169852564E-5 2.5168712169852564E-5 0.0 6.764204654852641 3.397776142930096E-4 88-89 2.5168712169852564E-5 2.5168712169852564E-5 0.0 7.350635648410206 3.523619703779359E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3670 0.0 50.81898 2 ACCTGGG 3785 0.0 49.148643 3 GTACCTG 4345 0.0 43.57842 1 CCTGGGG 3685 0.0 38.53618 4 CTGGGGG 3395 0.0 35.97156 5 GTACACG 565 0.0 32.67514 1 TGGGGGG 8190 0.0 29.244623 6 GTACCCG 275 0.0 25.820213 1 CATGGGG 7905 0.0 25.149672 4 ATGGGGG 6900 0.0 24.559149 5 CTGTGCG 2565 0.0 24.360592 9 GTACATG 12330 0.0 24.148428 1 GGGGGGA 8380 0.0 24.119219 7 TACATGG 12470 0.0 23.49741 2 ACATGGG 12415 0.0 23.105257 3 GTACTAG 2195 0.0 22.85981 1 CTAACGC 935 0.0 22.275423 3 CGCCTAC 1030 0.0 22.058878 7 ACTGTGC 3465 0.0 21.720675 8 GTATCAA 12215 0.0 21.585545 1 >>END_MODULE