FastQCFastQC Report
Sat 18 Jun 2016
SRR1931054_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931054_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425966
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT61580.4318476036595543No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33960.2381543458960452No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA31810.22307684755457002No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA27110.19011673490111264No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC25640.17980793370949938No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25210.17679243404120434No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25180.1765820503434163No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC20690.1450946235744751No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20330.1425700192010188No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC20330.1425700192010188No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT19980.1401155427268252No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC16450.1153603942871008No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT15660.10982029024534946No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT15100.10589312788663965No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14640.10266724452055659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG18650.053.5560762
ACCTGGG19550.050.6063083
GTACCTG20450.049.5365261
CCTGGGG17950.041.9312484
CTGGGGG17100.037.648525
TAACGCA2250.031.5583174
GTACTAG6750.028.051841
TGGGGGG36150.027.2370556
GTACCCG1755.14774E-927.0499861
CTGTGCG10450.026.7264239
GAGTACG2550.025.9892021
TAGTACT11600.024.4849014
AGGGCTA16900.024.3689965
GGGCTAT16750.024.3046156
GCTATTG15400.024.2835128
GTACGTG1951.6287231E-824.2756291
CATGGGG30550.023.3975754
CTATTGA16000.023.3728799
GGGGAAT33850.023.2142419
GGCTATT16550.023.168197
Produced by FastQC (version 0.11.5)