##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931054_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1425966 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.202347040532523 33.0 33.0 33.0 27.0 33.0 2 30.074818754444355 33.0 33.0 33.0 27.0 33.0 3 30.120150129806742 33.0 33.0 33.0 27.0 33.0 4 33.58349708197811 37.0 37.0 37.0 27.0 37.0 5 33.53560393445566 37.0 37.0 37.0 27.0 37.0 6 33.64918448265947 37.0 37.0 37.0 27.0 37.0 7 33.60740718923172 37.0 37.0 37.0 27.0 37.0 8 33.5970892714132 37.0 37.0 37.0 27.0 37.0 9 33.62890559802969 37.0 37.0 37.0 27.0 37.0 10-11 33.48564516966043 37.0 37.0 37.0 27.0 37.0 12-13 33.37059509132756 37.0 37.0 37.0 27.0 37.0 14-15 34.74188970845027 37.0 37.0 40.0 22.0 40.0 16-17 34.67772197934593 37.0 37.0 40.0 22.0 40.0 18-19 34.61031118554018 37.0 37.0 40.0 22.0 40.0 20-21 34.49464433233331 37.0 37.0 40.0 22.0 40.0 22-23 34.36620543547322 37.0 35.0 40.0 22.0 40.0 24-25 34.20746883165518 37.0 33.0 40.0 15.0 40.0 26-27 33.989235016823685 37.0 33.0 40.0 15.0 40.0 28-29 33.836920375380615 37.0 33.0 40.0 15.0 40.0 30-31 33.65292790992211 37.0 33.0 40.0 15.0 40.0 32-33 33.428382584156985 37.0 33.0 40.0 15.0 40.0 34-35 33.22600819374375 37.0 33.0 40.0 10.5 40.0 36-37 32.94457231098077 37.0 33.0 40.0 6.0 40.0 38-39 32.70984756999816 37.0 33.0 40.0 6.0 40.0 40-41 32.460656845955654 37.0 33.0 40.0 6.0 40.0 42-43 32.21008249846069 37.0 33.0 40.0 4.0 40.0 44-45 31.86506761030768 37.0 33.0 40.0 2.0 40.0 46-47 31.634844379178745 37.0 33.0 40.0 2.0 40.0 48-49 31.542003455902876 37.0 33.0 40.0 2.0 40.0 50-51 30.739125266661336 37.0 30.0 38.5 2.0 40.0 52-53 30.889183542945624 37.0 33.0 38.5 2.0 40.0 54-55 31.302136586706837 37.0 33.0 40.0 2.0 40.0 56-57 31.198064329724552 37.0 33.0 40.0 2.0 40.0 58-59 31.001392740079357 37.0 33.0 40.0 2.0 40.0 60-61 30.96073048024988 37.0 33.0 40.0 2.0 40.0 62-63 30.76954920383796 37.0 33.0 40.0 2.0 40.0 64-65 30.508031397663057 37.0 27.0 38.5 2.0 40.0 66-67 30.204634963245965 37.0 27.0 37.0 2.0 40.0 68-69 29.914776018502543 37.0 27.0 37.0 2.0 40.0 70-71 29.635657512170695 37.0 27.0 37.0 2.0 40.0 72-73 29.0359331849427 37.0 27.0 37.0 2.0 40.0 74-75 28.85821681582871 35.0 27.0 37.0 2.0 40.0 76-77 28.517911717390177 33.0 27.0 37.0 2.0 37.0 78-79 28.244973232180854 33.0 27.0 37.0 2.0 37.0 80-81 27.977590629790612 33.0 27.0 37.0 2.0 37.0 82-83 27.688788512489076 33.0 27.0 37.0 2.0 37.0 84-85 27.276605122422275 33.0 24.5 37.0 2.0 37.0 86-87 27.109413197790133 33.0 22.0 37.0 2.0 37.0 88-89 26.888316411471244 33.0 22.0 37.0 2.0 37.0 90-91 26.56194222022124 33.0 22.0 37.0 2.0 37.0 92-93 26.238847910819754 33.0 22.0 37.0 2.0 37.0 94-95 25.94100420346628 33.0 15.0 37.0 2.0 37.0 96-97 25.581810155361346 33.0 15.0 37.0 2.0 37.0 98-99 25.220437934705316 33.0 4.0 37.0 2.0 37.0 100-101 24.502367517879108 33.0 2.0 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2709659610167101 1101 2 -0.15878802556271054 1101 3 -0.26613833992815117 1101 4 -0.2190860937822734 1101 5 -0.3730996442641512 1101 6 -0.18198952371736965 1101 7 -0.13764254972254975 1101 8 -0.271392761222053 1101 9 -0.34662346805411914 1101 10-11 -0.2035467469045571 1101 12-13 -0.06221437346586356 1101 14-15 -0.06850935976295602 1101 16-17 -0.1535860488334393 1101 18-19 -0.2953423659747685 1101 20-21 -0.2756962527239466 1101 22-23 -0.3079524386406547 1101 24-25 -0.3150703911760786 1101 26-27 -0.370132541861949 1101 28-29 -0.22941255145645556 1101 30-31 -0.28880369081576873 1101 32-33 -0.27768398987913656 1101 34-35 -0.04498227723001946 1101 36-37 0.03239587504270247 1101 38-39 -0.17721475709284107 1101 40-41 -0.025572170356213064 1101 42-43 -0.21168625033114807 1101 44-45 -0.19425722570547066 1101 46-47 -0.18168451871505553 1101 48-49 -0.1946354315969856 1101 50-51 -0.059666776709136116 1101 52-53 0.12769846542125762 1101 54-55 0.1452021239291703 1101 56-57 -0.04963632182733946 1101 58-59 -0.0030867087153971795 1101 60-61 -0.07614531699890392 1101 62-63 -0.01293768166750553 1101 64-65 -0.04204589613733134 1101 66-67 0.10733397186903915 1101 68-69 0.23749298171169997 1101 70-71 0.09655424289017134 1101 72-73 0.1398206471833987 1101 74-75 -0.049009030386603314 1101 76-77 0.08523850190396232 1101 78-79 0.14621088301439755 1101 80-81 0.26330021581564367 1101 82-83 0.11675894618440807 1101 84-85 0.21487428177750445 1101 86-87 0.24914088090613618 1101 88-89 0.09920635205894257 1101 90-91 0.09493668839117575 1101 92-93 0.26251230146305815 1101 94-95 0.07520303188418964 1101 96-97 -0.010351660733448398 1101 98-99 -0.08099582618774903 1101 100-101 -0.03808005303754314 1102 1 0.5950117388566944 1102 2 0.5335651733855791 1102 3 0.5078172545795923 1102 4 0.4807678355195151 1102 5 0.38208343101094755 1102 6 0.5055822943759267 1102 7 0.5718203970103417 1102 8 0.6624737301597747 1102 9 0.5690986080974056 1102 10-11 0.4775748326065994 1102 12-13 0.5164666081292353 1102 14-15 0.5973186176447811 1102 16-17 0.6321331059041171 1102 18-19 0.6799619479798551 1102 20-21 0.7422083631101088 1102 22-23 0.548901928873228 1102 24-25 0.4478653864069386 1102 26-27 0.5308850840022998 1102 28-29 0.4119656255812316 1102 30-31 0.49756669578467694 1102 32-33 0.5404750324209857 1102 34-35 0.7021462162941816 1102 36-37 0.6192957679241928 1102 38-39 0.6479751348148426 1102 40-41 0.604657160639988 1102 42-43 0.5789725285178093 1102 44-45 0.872353447193543 1102 46-47 0.8491802198183898 1102 48-49 0.7604685220499121 1102 50-51 0.6840107965696625 1102 52-53 0.8047799728708647 1102 54-55 0.8204224472323141 1102 56-57 0.7353147444887576 1102 58-59 0.8353943994742998 1102 60-61 0.8819896036360149 1102 62-63 0.9054136670519419 1102 64-65 0.9339072151685421 1102 66-67 0.9870776177936698 1102 68-69 1.0525528706979692 1102 70-71 0.858153473584494 1102 72-73 0.6266450702041375 1102 74-75 0.6353784464855359 1102 76-77 0.7517858775004171 1102 78-79 0.6124076889351322 1102 80-81 0.7875576897663699 1102 82-83 0.4903724918071539 1102 84-85 0.6262178887499452 1102 86-87 0.4739923756962803 1102 88-89 0.5751460735508118 1102 90-91 0.4042146122396524 1102 92-93 0.5524991551002181 1102 94-95 0.46351250700348245 1102 96-97 0.5521069525687281 1102 98-99 0.6515414218402995 1102 100-101 0.580333375728646 1103 1 0.14864328198083854 1103 2 0.09140473519976311 1103 3 0.10628259246279725 1103 4 0.10306711532746249 1103 5 0.11501106228732993 1103 6 0.1276044888001806 1103 7 0.049939849554007765 1103 8 0.14966456725656485 1103 9 0.08712870347915924 1103 10-11 0.20044173504494722 1103 12-13 0.2778156115416408 1103 14-15 0.23890062572048976 1103 16-17 0.3331039395057189 1103 18-19 0.3566435458312327 1103 20-21 0.41887788877673415 1103 22-23 0.31398123737847783 1103 24-25 0.2599986219364112 1103 26-27 0.3716828599609485 1103 28-29 0.41298946182261886 1103 30-31 0.2997133601825439 1103 32-33 0.4444448266649701 1103 34-35 0.28379500079763176 1103 36-37 0.39283767795077296 1103 38-39 0.4322394443778492 1103 40-41 0.49680771113160915 1103 42-43 0.547912521711396 1103 44-45 0.5922983060524594 1103 46-47 0.17042335591905555 1103 48-49 0.3606785780648494 1103 50-51 0.7042679226078725 1103 52-53 0.6551561651428237 1103 54-55 0.5824561159807651 1103 56-57 0.4859476718900879 1103 58-59 0.4231275651000743 1103 60-61 0.5273231868444128 1103 62-63 0.5661346869101109 1103 64-65 0.634517607824975 1103 66-67 0.6865854175747579 1103 68-69 0.657215263427986 1103 70-71 0.6973835901213157 1103 72-73 0.49166079853629086 1103 74-75 0.5370556753557629 1103 76-77 0.7845221933915525 1103 78-79 0.7202214967551477 1103 80-81 0.9063721322383849 1103 82-83 0.8780580585329965 1103 84-85 0.9242243686388356 1103 86-87 0.7731675580437596 1103 88-89 0.7622545898448116 1103 90-91 0.8250900640842822 1103 92-93 0.5054176345108168 1103 94-95 0.4790177911712128 1103 96-97 0.5474718084843246 1103 98-99 0.5388745957441436 1103 100-101 0.6743095854765322 1104 1 -0.11864946758346662 1104 2 0.003394715554041028 1104 3 -0.1768224105106988 1104 4 -0.09728285472014875 1104 5 -0.12167751873035115 1104 6 -0.16233357335075738 1104 7 -0.21459847591705028 1104 8 -0.29816336794189624 1104 9 -0.3368982243187233 1104 10-11 -0.24322928658709486 1104 12-13 -0.3527509942055218 1104 14-15 -0.2587901590539019 1104 16-17 -0.13491712610360906 1104 18-19 -0.12733817614316933 1104 20-21 -0.22955170353371557 1104 22-23 -0.12456471807019653 1104 24-25 -0.036932046159556364 1104 26-27 0.014068972923318768 1104 28-29 -0.04114206559713551 1104 30-31 -0.09187556254246232 1104 32-33 -0.13484094741680508 1104 34-35 -0.006846752292460678 1104 36-37 -0.16767865561558892 1104 38-39 -0.15258942472639347 1104 40-41 -0.19804618534291762 1104 42-43 -0.22602030882764979 1104 44-45 -0.2393100014689047 1104 46-47 -0.08893010730582418 1104 48-49 0.002818439968560682 1104 50-51 -0.1952078290014576 1104 52-53 0.036291648887537775 1104 54-55 0.2552536910873364 1104 56-57 0.20511953990822107 1104 58-59 0.20565553606745723 1104 60-61 0.33426480305588413 1104 62-63 0.2214068514079912 1104 64-65 0.39495555418899286 1104 66-67 0.40938255780087474 1104 68-69 0.31038396551639025 1104 70-71 0.4147759816702852 1104 72-73 0.41350326743962285 1104 74-75 0.40230633501505864 1104 76-77 0.3791793608194389 1104 78-79 0.3200284641923865 1104 80-81 0.34031052924727945 1104 82-83 0.34402105238052627 1104 84-85 0.36106314649429194 1104 86-87 0.5100686868847042 1104 88-89 0.5089799400051191 1104 90-91 0.3510018725020494 1104 92-93 0.44933246099895285 1104 94-95 0.4990165843779444 1104 96-97 0.4910422640107335 1104 98-99 0.49617966329738294 1104 100-101 0.34966670387165166 1105 1 -0.02966239520889502 1105 2 -0.16461562572458988 1105 3 -0.14142499850092705 1105 4 -0.054834104034526376 1105 5 -0.11989131655158047 1105 6 -0.20886573217998006 1105 7 -0.2810685131201822 1105 8 -0.2183440629892175 1105 9 -0.12566928845971148 1105 10-11 -0.1890002295251776 1105 12-13 -0.1080359032109044 1105 14-15 -0.06512229199911701 1105 16-17 -0.2823553280276556 1105 18-19 -0.3119674390446008 1105 20-21 -0.2186714314640028 1105 22-23 -0.20427309154771223 1105 24-25 -0.24463556946646747 1105 26-27 -0.21440723946525964 1105 28-29 -0.28455217104366426 1105 30-31 -0.14688858671936345 1105 32-33 -0.11814781469140456 1105 34-35 -0.20634519822466757 1105 36-37 -0.03703732788908809 1105 38-39 -0.2341417771926686 1105 40-41 -0.22270558462844292 1105 42-43 -0.2610859535552166 1105 44-45 -0.25267262208120655 1105 46-47 -0.12735091558542067 1105 48-49 -0.161876539560474 1105 50-51 -0.3665679413298477 1105 52-53 -0.14327482474466535 1105 54-55 -0.03928683690540069 1105 56-57 -0.11196601291057107 1105 58-59 -0.10063268650216983 1105 60-61 -0.248144957270501 1105 62-63 -0.11806605974205908 1105 64-65 0.054524318350733125 1105 66-67 0.03999281444289693 1105 68-69 -0.07419594315003053 1105 70-71 -0.16492109323105453 1105 72-73 -0.10724194555908184 1105 74-75 0.0013237183880718817 1105 76-77 0.0501661065624468 1105 78-79 0.11160388629692264 1105 80-81 0.11496171138648847 1105 82-83 -0.02158135688677376 1105 84-85 0.09324785972615501 1105 86-87 0.16192360494948232 1105 88-89 0.1387225173541431 1105 90-91 0.06852925100640306 1105 92-93 -0.0856766908477482 1105 94-95 -0.06540918089516268 1105 96-97 -0.06054164229736614 1105 98-99 -0.09057696742611299 1105 100-101 -0.03878039927613486 1106 1 0.10983481736068867 1106 2 0.139995194068689 1106 3 0.1472784879576743 1106 4 0.11625314526331465 1106 5 0.07252820535514104 1106 6 0.19465293179180065 1106 7 0.2550545053408655 1106 8 0.18564657625206848 1106 9 0.24627044408426713 1106 10-11 0.27433511622320594 1106 12-13 0.1693964604614422 1106 14-15 0.28878142302676224 1106 16-17 0.1945093023980391 1106 18-19 0.10653908986250116 1106 20-21 0.3262315889758085 1106 22-23 0.2450402980120927 1106 24-25 0.35675557133056657 1106 26-27 0.397962752801412 1106 28-29 0.48399494268874577 1106 30-31 0.39465154682692827 1106 32-33 0.4170650739183941 1106 34-35 0.24899887427073253 1106 36-37 0.4214954801971871 1106 38-39 0.472842093872778 1106 40-41 0.4532495721683034 1106 42-43 0.4954141807179653 1106 44-45 0.3921975367649786 1106 46-47 0.24609863303459534 1106 48-49 0.14239632574838623 1106 50-51 0.18406490936039788 1106 52-53 0.16990903460973428 1106 54-55 0.416199080564045 1106 56-57 0.3713348963106675 1106 58-59 0.43640576206716375 1106 60-61 0.5467704304357284 1106 62-63 0.4865085163888132 1106 64-65 0.488131784343274 1106 66-67 0.47465654594499895 1106 68-69 0.6729696813010158 1106 70-71 0.6097634657572719 1106 72-73 0.2756274217820476 1106 74-75 0.5101920579841241 1106 76-77 0.4117372743756498 1106 78-79 0.6289208356103089 1106 80-81 0.7074756788257481 1106 82-83 0.5900299414439978 1106 84-85 0.7679029391896286 1106 86-87 0.6916181688039522 1106 88-89 0.6468061665154927 1106 90-91 0.7557313008429531 1106 92-93 0.8326044509386712 1106 94-95 0.8595263074049022 1106 96-97 1.0256958112370498 1106 98-99 1.173364727727332 1106 100-101 1.102572912829249 1107 1 -0.4031961829021036 1107 2 -0.39092140942023335 1107 3 -0.20876180841368708 1107 4 -0.46047803517421215 1107 5 -0.5226028230776762 1107 6 -0.5425027652650911 1107 7 -0.4918950022508639 1107 8 -0.4839417065296203 1107 9 -0.49074582079715867 1107 10-11 -0.44771015055071217 1107 12-13 -0.4214981930945072 1107 14-15 -0.3745522632196483 1107 16-17 -0.42864499371997766 1107 18-19 -0.4043125146000861 1107 20-21 -0.2601095405855176 1107 22-23 -0.305814633916647 1107 24-25 -0.4616021161560795 1107 26-27 -0.43201625202151916 1107 28-29 -0.37909045975505506 1107 30-31 -0.5413372683493449 1107 32-33 -0.42471674639465107 1107 34-35 -0.3625404853054661 1107 36-37 -0.5463850183933658 1107 38-39 -0.4677288407103717 1107 40-41 -0.4226796605671552 1107 42-43 -0.4722552790036332 1107 44-45 -0.4076440304715838 1107 46-47 -0.6300898490169367 1107 48-49 -0.5603368757467067 1107 50-51 -0.5619066823973178 1107 52-53 -0.5394292344650609 1107 54-55 -0.4166068525349793 1107 56-57 -0.5677081924854157 1107 58-59 -0.4627852477242804 1107 60-61 -0.46731410989183786 1107 62-63 -0.41227034134499263 1107 64-65 -0.6609356270960269 1107 66-67 -0.3769309011889348 1107 68-69 -0.16322067245022964 1107 70-71 -0.17425751240434195 1107 72-73 -0.30072185629014214 1107 74-75 -0.4549435173555736 1107 76-77 -0.4373668620807116 1107 78-79 -0.3933611350058932 1107 80-81 -0.326242329761385 1107 82-83 -0.36992371980860383 1107 84-85 -0.2718746972128514 1107 86-87 -0.14260051945595364 1107 88-89 -0.36092901066262684 1107 90-91 -0.23620621085516902 1107 92-93 -0.16373817220121012 1107 94-95 -0.3665594202230871 1107 96-97 -0.34871951892889186 1107 98-99 -0.5254634275519017 1107 100-101 -0.4773741880213329 1108 1 -0.4359617839072705 1108 2 -0.4747654691717358 1108 3 -0.4977486156173718 1108 4 -0.43208734691510386 1108 5 -0.5196117578815276 1108 6 -0.7342869339095159 1108 7 -0.687099525495313 1108 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11 7359.0 12 8150.0 13 8854.0 14 8995.0 15 9985.0 16 11388.0 17 13857.0 18 12418.0 19 9708.0 20 9542.0 21 9854.0 22 10551.0 23 11653.0 24 13079.0 25 14914.0 26 17256.0 27 19968.0 28 23650.0 29 27891.0 30 33662.0 31 41439.0 32 51556.0 33 67255.0 34 89993.0 35 124837.0 36 182473.0 37 270900.0 38 187867.0 39 252.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.42043712490024 20.075682665780242 12.042955102338503 23.46092510698101 2 15.552415282764812 21.09325908886894 40.480391606822266 22.873934021543985 3 19.35012202305815 28.006185306740722 29.08300373081994 23.560688939381187 4 12.229635624350431 16.913122937334233 39.9595075830361 30.89773385527923 5 12.663883467502421 37.89865891690867 34.68259817930245 14.754859436286456 6 28.21750308212117 38.210868982850926 19.525149968512572 14.046477966515331 7 26.322156348748848 31.920887314283792 24.278418980536703 17.47853735643066 8 25.644720841871404 33.7832038071034 21.172734833789868 19.399340517235334 9 26.451472194989222 15.792732786055208 22.294009815100782 35.46178520385479 10-11 25.054489377727094 25.87428452010777 28.718882497899667 20.35234360426546 12-13 25.845235816076574 24.273806543367535 28.353926434276346 21.527031206279545 14-15 22.532413454542265 25.705329043682557 27.08907453998755 24.673182961787628 16-17 21.322815980812084 27.752450649503206 28.06211526424585 22.862618105438855 18-19 20.99415741936208 28.251622997508257 29.525650619148042 21.228568963981623 20-21 22.33124772960926 27.008463034883018 28.715866998231377 21.944422237276346 22-23 22.076052304192388 27.146369548783074 28.35141230576325 22.42616584126129 24-25 22.256947220340457 27.122876702530075 29.004618623445438 21.61555745368403 26-27 22.118935514591513 26.83289783907891 29.055356158561985 21.992810487767592 28-29 21.328383706203375 28.004699971808584 28.81243311551608 21.854483206471965 30-31 22.307299052011057 27.124735091860536 28.472979019135096 22.094986836993307 32-33 22.524029324682356 27.445990998382854 28.121077220634994 21.9089024562998 34-35 22.630763987360147 26.92935876451472 28.853843640030686 21.586033608094443 36-37 22.590650828981897 26.75502080694771 28.8044034710505 21.84992489301989 38-39 22.437316177244057 27.147877298617217 28.873023620479028 21.541782903659694 40-41 22.15260445411901 26.862586003404022 28.78150243940753 22.203307103069438 42-43 22.125991126713796 27.093222101095716 27.97774560464471 22.80304116754578 44-45 22.329877170036923 27.337117931491527 28.00467055411987 22.32833434435168 46-47 22.685519606786915 27.214675904748614 27.817529631389228 22.28227485707524 48-49 22.989933584996646 26.895485464734058 27.881153064004945 22.23342788626435 50-51 22.062720086073416 27.331442497199305 27.93629412547538 22.6695432912519 52-53 21.984882853195213 27.898007285193916 27.36166276652301 22.75544709508786 54-55 21.99067632362466 27.50006068727548 27.556033704833276 22.953229284266584 56-57 22.924159983568774 26.793164582693684 27.449358454255112 22.833316979482422 58-59 22.871328357505558 26.955565731360164 27.720017191013685 22.45308872012059 60-61 22.402372393986454 27.797747364934544 27.66726428923291 22.132615951846084 62-63 22.28076226519378 27.315170801562616 27.424937777032405 22.979129156211204 64-65 21.579199578670103 27.946364816703568 27.437457085630452 23.03697851899588 66-67 22.28943997367692 26.917528049125167 27.159526015836033 23.63350596136188 68-69 21.771389810493165 27.28343080470112 27.278264353596132 23.66691503120958 70-71 22.28004171721088 26.938533900858 27.509140139668997 23.272284242262124 72-73 22.757400741930077 26.535948682816052 28.08085864231179 22.62579193294208 74-75 22.358177554608922 27.703372491289223 27.24019605769488 22.69825389640697 76-77 22.46757311508752 26.876408570104843 27.333361326835547 23.322656987972085 78-79 22.191292047355894 27.093179351029335 27.61519202923412 23.100336572380655 80-81 22.130917774999254 26.97480159044187 27.955163748335966 22.939116886222912 82-83 22.50748386919818 26.33892951747725 28.571694816376635 22.58189179694794 84-85 22.590218152244198 26.986666422880546 27.555860131932832 22.867255292942428 86-87 21.882300749944985 27.44997613140313 27.672245848656924 22.99547726999496 88-89 22.374604348614746 26.83585055029114 27.31906902578712 23.470476075306994 90-91 22.44172511177198 26.336209972764095 27.750494215490257 23.471570699973668 92-93 22.757801754160315 26.86441910922842 27.59685378506581 22.78092535154545 94-95 21.647750447128207 26.844871542199733 28.069147398260107 23.43823061241195 96-97 21.617553846180858 26.931149578869928 27.9693576734868 23.48193890146241 98-99 21.666788412425934 27.488670620594323 27.196024251755247 23.648516715224492 100-101 21.80222941590034 26.719909873779745 27.243445445612775 24.234415264707142 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 102.0 1 72.5 2 85.5 3 124.5 4 99.5 5 86.0 6 130.0 7 228.5 8 345.5 9 387.0 10 390.5 11 413.0 12 464.0 13 557.5 14 683.5 15 815.0 16 975.5 17 1192.0 18 1429.0 19 1647.0 20 1849.5 21 2154.0 22 2528.5 23 2971.0 24 3650.5 25 4530.5 26 5941.0 27 7760.5 28 10029.0 29 13770.0 30 18632.5 31 22902.5 32 27766.5 33 32494.0 34 36562.5 35 40257.5 36 42944.0 37 47830.0 38 49082.5 39 45699.0 40 43141.5 41 40918.5 42 40215.0 43 41217.0 44 43053.0 45 46599.5 46 50624.0 47 55380.0 48 63639.0 49 64002.0 50 56468.0 51 51837.5 52 48078.5 53 45446.0 54 43297.0 55 39340.5 56 35605.0 57 30886.5 58 26032.5 59 23673.0 60 20533.5 61 16462.5 62 13136.5 63 10791.5 64 8661.5 65 6477.0 66 5138.5 67 4252.5 68 3470.5 69 2881.5 70 2483.5 71 2087.0 72 1725.0 73 1482.0 74 1276.5 75 1112.5 76 940.5 77 776.5 78 640.5 79 502.0 80 410.5 81 357.0 82 298.0 83 242.0 84 195.0 85 149.5 86 119.5 87 91.5 88 66.5 89 56.0 90 42.0 91 30.5 92 22.0 93 17.5 94 15.0 95 14.0 96 9.5 97 3.5 98 4.0 99 4.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 8.415347911521033E-4 2 0.0032258833660830624 3 4.2076739557605163E-4 4 0.0014025579852535053 5 0.002033709078617583 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 2.1038369778802581E-4 14-15 0.0037518426105531267 16-17 0.004803761099493256 18-19 0.0038570344594471397 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 3.5063949631337633E-4 42-43 3.5063949631337636E-5 44-45 7.71406891889428E-4 46-47 0.7327313554460625 48-49 0.5285189127931521 50-51 0.4055496414360511 52-53 0.3411371659632838 54-55 0.33279194595102546 56-57 0.30032272859240683 58-59 0.30105907153466493 60-61 0.2764792428430972 62-63 0.2708690109020832 64-65 0.2666613369463227 66-67 0.255265553316138 68-69 0.23324539294765795 70-71 0.24765667624613769 72-73 0.8097668527861113 74-75 0.5335330575904335 76-77 0.6290823203358286 78-79 0.35228750194604924 80-81 0.30495116994374344 82-83 0.2857361255457704 84-85 0.27732077763424934 86-87 0.2521097978493176 88-89 0.25442401852498586 90-91 0.26385622097581574 92-93 0.22279633595751933 94-95 0.19050243834705735 96-97 0.14856595458797756 98-99 0.15666572695281655 100-101 0.15322945988894546 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1425966.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.21705900807073 #Duplication Level Percentage of deduplicated Percentage of total 1 90.34010898430674 54.400156735035395 2 5.846070195765316 7.040663078874472 3 1.4024068051095342 2.533464400098023 4 0.5683060506479977 1.3688687594605644 5 0.3201786874406272 0.9640109457369441 6 0.2072542461247593 0.7488144701140705 7 0.16036715210273278 0.6759786782788559 8 0.13003145789367643 0.6264089578311183 9 0.09226586820116799 0.5000381306910548 >10 0.7138386436137567 8.8186020422936 >50 0.11236125791933221 4.735851312290634 >100 0.09678260734867233 11.13027172028154 >500 0.0066464939647626845 2.697074318524804 >1k 0.0032649444037430734 3.3274051132814404 >5k 1.1660515727653832E-4 0.4323913372074599 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6158 0.4318476036595543 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3396 0.2381543458960452 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 3181 0.22307684755457002 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 2711 0.19011673490111264 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 2564 0.17980793370949938 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2521 0.17679243404120434 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2518 0.1765820503434163 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 2069 0.1450946235744751 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2033 0.1425700192010188 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 2033 0.1425700192010188 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1998 0.1401155427268252 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 1645 0.1153603942871008 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 1566 0.10982029024534946 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 1510 0.10589312788663965 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1464 0.10266724452055659 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 7.012789926267527E-5 0.0 6 0.0 0.0 0.0 7.012789926267527E-5 7.012789926267527E-5 7 0.0 0.0 0.0 7.012789926267527E-5 7.012789926267527E-5 8 0.0 0.0 0.0 7.012789926267527E-5 7.012789926267527E-5 9 0.0 0.0 0.0 7.012789926267527E-5 7.012789926267527E-5 10-11 0.0 0.0 0.0 7.012789926267527E-5 7.012789926267527E-5 12-13 0.0 0.0 0.0 7.012789926267527E-5 7.012789926267527E-5 14-15 0.0 0.0 0.0 7.012789926267527E-5 7.012789926267527E-5 16-17 0.0 0.0 0.0 7.012789926267527E-5 7.012789926267527E-5 18-19 0.0 3.5063949631337636E-5 0.0 7.012789926267527E-5 1.0519184889401291E-4 20-21 0.0 7.012789926267527E-5 0.0 1.0519184889401291E-4 2.1038369778802581E-4 22-23 0.0 7.012789926267527E-5 0.0 2.805115970507011E-4 2.1038369778802581E-4 24-25 7.012789926267527E-5 7.012789926267527E-5 0.0 7.363429422580903E-4 2.1038369778802581E-4 26-27 7.012789926267527E-5 7.012789926267527E-5 0.0 0.001157110337834142 2.1038369778802581E-4 28-29 7.012789926267527E-5 7.012789926267527E-5 0.0 0.0029453717690323612 2.1038369778802581E-4 30-31 7.012789926267527E-5 7.012789926267527E-5 0.0 0.007679004969262942 2.1038369778802581E-4 32-33 7.012789926267527E-5 7.012789926267527E-5 0.0 0.020056579189125127 2.1038369778802581E-4 34-35 7.012789926267527E-5 7.012789926267527E-5 0.0 0.044285768384379434 2.1038369778802581E-4 36-37 7.012789926267527E-5 7.012789926267527E-5 0.0 0.0791042703682977 2.1038369778802581E-4 38-39 7.012789926267527E-5 7.012789926267527E-5 0.0 0.1289301427944285 2.1038369778802581E-4 40-41 7.012789926267527E-5 1.4025579852535054E-4 0.0 0.18261304968000638 2.1038369778802581E-4 42-43 7.012789926267527E-5 1.4025579852535054E-4 0.0 0.236576468162635 2.1038369778802581E-4 44-45 7.012789926267527E-5 1.4025579852535054E-4 0.0 0.3027070771673378 2.1038369778802581E-4 46-47 7.012789926267527E-5 1.4025579852535054E-4 0.0 0.38486191115356183 2.1038369778802581E-4 48-49 7.012789926267527E-5 1.4025579852535054E-4 0.0 0.46736738463609934 2.1038369778802581E-4 50-51 7.012789926267527E-5 1.4025579852535054E-4 0.0 0.5548870029159181 2.1038369778802581E-4 52-53 7.012789926267527E-5 1.4025579852535054E-4 0.0 0.6401274644696999 2.1038369778802581E-4 54-55 7.012789926267527E-5 1.4025579852535054E-4 0.0 0.7373948607470304 2.1038369778802581E-4 56-57 7.012789926267527E-5 2.1038369778802581E-4 0.0 0.8595225973129794 2.1038369778802581E-4 58-59 7.012789926267527E-5 2.1038369778802581E-4 0.0 0.9919240711209103 2.1038369778802581E-4 60-61 7.012789926267527E-5 2.1038369778802581E-4 0.0 1.1266397656045095 2.1038369778802581E-4 62-63 7.012789926267527E-5 2.1038369778802581E-4 0.0 1.2702266393448371 2.1038369778802581E-4 64-65 7.012789926267527E-5 2.1038369778802581E-4 0.0 1.4216678378025844 2.1038369778802581E-4 66-67 7.012789926267527E-5 2.1038369778802581E-4 0.0 1.5912020342701019 2.1038369778802581E-4 68-69 1.4025579852535054E-4 2.4544764741936344E-4 0.0 1.7816343447178964 2.4544764741936344E-4 70-71 1.4025579852535054E-4 2.805115970507011E-4 0.0 1.9919479146066597 2.805115970507011E-4 72-73 1.4025579852535054E-4 2.805115970507011E-4 0.0 2.2086080593786948 2.805115970507011E-4 74-75 1.4025579852535054E-4 3.1557554668203874E-4 0.0 2.4282837038190253 2.805115970507011E-4 76-77 1.4025579852535054E-4 3.5063949631337633E-4 0.0 2.676992298554103 2.805115970507011E-4 78-79 1.4025579852535054E-4 3.5063949631337633E-4 0.0 2.9559961457706567 2.805115970507011E-4 80-81 1.4025579852535054E-4 3.5063949631337633E-4 0.0 3.262805705044861 2.805115970507011E-4 82-83 1.4025579852535054E-4 3.5063949631337633E-4 0.0 3.5744540893681895 2.805115970507011E-4 84-85 1.4025579852535054E-4 3.5063949631337633E-4 0.0 3.8873297119286154 2.805115970507011E-4 86-87 1.4025579852535054E-4 3.5063949631337633E-4 0.0 4.227099383856277 2.805115970507011E-4 88-89 1.4025579852535054E-4 3.5063949631337633E-4 0.0 4.591834587921451 2.805115970507011E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1865 0.0 53.556076 2 ACCTGGG 1955 0.0 50.606308 3 GTACCTG 2045 0.0 49.536526 1 CCTGGGG 1795 0.0 41.931248 4 CTGGGGG 1710 0.0 37.64852 5 TAACGCA 225 0.0 31.558317 4 GTACTAG 675 0.0 28.05184 1 TGGGGGG 3615 0.0 27.237055 6 GTACCCG 175 5.14774E-9 27.049986 1 CTGTGCG 1045 0.0 26.726423 9 GAGTACG 255 0.0 25.989202 1 TAGTACT 1160 0.0 24.484901 4 AGGGCTA 1690 0.0 24.368996 5 GGGCTAT 1675 0.0 24.304615 6 GCTATTG 1540 0.0 24.283512 8 GTACGTG 195 1.6287231E-8 24.275629 1 CATGGGG 3055 0.0 23.397575 4 CTATTGA 1600 0.0 23.372879 9 GGGGAAT 3385 0.0 23.214241 9 GGCTATT 1655 0.0 23.16819 7 >>END_MODULE