FastQCFastQC Report
Sat 18 Jun 2016
SRR1931054_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931054_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425966
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT72300.5070247116691422No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36770.25786028558885693No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA34600.24264253144885645No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32460.2276351610066439No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA30340.21276804636295676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29250.20512410534332515No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC26550.18618957254240281No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25200.17672230614194168No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC24600.17251463218618115No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC24460.1715328415965037No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC22770.1596812266211116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22400.15708649434839259No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT17640.12370561429935917No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16670.11690320807087966No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16040.11248515041733112No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15730.11031118554018819No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT15190.10652427898000373No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC15140.10617363948369037No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC14960.1049113372969622No Hit
GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA14300.10028289594562563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG23450.055.7017752
ACCTGGG23600.055.146473
GTACCTG25050.052.5711331
CCTGGGG19950.049.5221634
CTGGGGG15450.049.1893045
ATAACGC2250.044.331863
TAACGCA2150.044.1845784
GTACACG3350.042.5747151
TATCACG1051.027729E-940.712932
TACACGG4450.035.223552
ACACGGG4500.033.7766533
GTACCCG1701.07320375E-1030.7623181
TGGGGGG32800.030.70026
GTACTAG7350.029.1072031
GGGGGGA34000.026.4035347
CTGTGCG11350.026.3647639
CGACGAG3800.026.2491324-25
TAACGCC3950.025.2523254
TAGTACT12300.025.1007944
TCTAACG3850.024.6745052
Produced by FastQC (version 0.11.5)