##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1931053_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5243212
Sequences flagged as poor quality	0
Sequence length	101
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.373453524290074	33.0	33.0	33.0	27.0	33.0
2	31.30602767921648	33.0	33.0	33.0	27.0	33.0
3	31.32122618730656	33.0	33.0	33.0	27.0	33.0
4	34.97952342953136	37.0	37.0	37.0	33.0	37.0
5	34.97928597966285	37.0	37.0	37.0	33.0	37.0
6	35.07091950506674	37.0	37.0	37.0	33.0	37.0
7	35.035387277874705	37.0	37.0	37.0	33.0	37.0
8	35.03370281422914	37.0	37.0	37.0	33.0	37.0
9	35.04265248096014	37.0	37.0	37.0	33.0	37.0
10-11	34.96345646523543	37.0	37.0	37.0	33.0	37.0
12-13	34.898428768472456	37.0	37.0	37.0	33.0	37.0
14-15	36.6201407648594	40.0	37.0	40.0	33.0	40.0
16-17	36.5838068344366	40.0	37.0	40.0	33.0	40.0
18-19	36.53206374260663	40.0	37.0	40.0	33.0	40.0
20-21	36.45618954564492	40.0	37.0	40.0	33.0	40.0
22-23	36.39090589890319	40.0	37.0	40.0	33.0	40.0
24-25	36.28729860627416	40.0	37.0	40.0	33.0	40.0
26-27	36.13386641623494	40.0	37.0	40.0	33.0	40.0
28-29	36.04766620155736	40.0	37.0	40.0	33.0	40.0
30-31	35.934723123917166	40.0	37.0	40.0	33.0	40.0
32-33	35.79536188885744	40.0	37.0	40.0	27.0	40.0
34-35	35.712865510683145	40.0	37.0	40.0	27.0	40.0
36-37	35.536511493336526	38.5	37.0	40.0	27.0	40.0
38-39	35.39483145064514	37.0	37.0	40.0	27.0	40.0
40-41	35.20128434631291	37.0	37.0	40.0	27.0	40.0
42-43	35.06998114895984	37.0	37.0	40.0	27.0	40.0
44-45	34.86925380854331	37.0	37.0	40.0	27.0	40.0
46-47	34.75002641510586	37.0	37.0	40.0	24.5	40.0
48-49	34.78958260699739	37.0	37.0	40.0	22.0	40.0
50-51	34.019171263721546	37.0	35.0	38.5	22.0	40.0
52-53	34.13632130457437	37.0	35.0	38.5	22.0	40.0
54-55	34.68500834984357	37.0	37.0	40.0	27.0	40.0
56-57	34.68307604193765	37.0	37.0	40.0	27.0	40.0
58-59	34.57290817155591	37.0	37.0	40.0	27.0	40.0
60-61	34.611416437100004	37.0	37.0	40.0	27.0	40.0
62-63	34.474483293828285	37.0	37.0	40.0	24.5	40.0
64-65	34.29010041936126	37.0	33.0	40.0	22.0	40.0
66-67	34.075907478087856	37.0	33.0	40.0	22.0	40.0
68-69	33.87921468748546	37.0	33.0	40.0	22.0	40.0
70-71	33.64792030152509	37.0	33.0	40.0	22.0	40.0
72-73	33.02862176848848	37.0	33.0	40.0	15.0	40.0
74-75	32.832092999482	37.0	33.0	37.0	15.0	40.0
76-77	32.446508552391165	37.0	33.0	37.0	15.0	40.0
78-79	32.1663520376441	37.0	33.0	37.0	15.0	38.5
80-81	31.88251857830658	37.0	33.0	37.0	15.0	37.0
82-83	31.598466646017748	37.0	33.0	37.0	10.5	37.0
84-85	31.20640696962091	37.0	33.0	37.0	6.0	37.0
86-87	31.019224475378834	37.0	33.0	37.0	4.0	37.0
88-89	30.801923897031056	37.0	33.0	37.0	2.0	37.0
90-91	30.508192687993542	37.0	33.0	37.0	2.0	37.0
92-93	30.2308280687487	33.0	33.0	37.0	2.0	37.0
94-95	29.979432550123853	33.0	33.0	37.0	2.0	37.0
96-97	29.688896710642254	33.0	33.0	37.0	2.0	37.0
98-99	29.356030234901812	33.0	30.0	37.0	2.0	37.0
100-101	28.649885604472985	33.0	27.0	37.0	2.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.15332187483669912
1101	2	0.18581735837691937
1101	3	0.13825609115944815
1101	4	0.2757893369645714
1101	5	0.1610533224912416
1101	6	0.14467351114274862
1101	7	0.1947589499332878
1101	8	0.20682795896548356
1101	9	0.13438162937541875
1101	10-11	0.14226949115507637
1101	12-13	0.25271879215215876
1101	14-15	0.38662615156754043
1101	16-17	0.4090887168271209
1101	18-19	0.3468670377796954
1101	20-21	0.3478867014984033
1101	22-23	0.3982596404289538
1101	24-25	0.361360942024028
1101	26-27	0.36121332841759113
1101	28-29	0.32155175610694187
1101	30-31	0.2684714492233127
1101	32-33	0.2917987738298109
1101	34-35	0.33632680147535154
1101	36-37	0.310506359707162
1101	38-39	0.2798543966045344
1101	40-41	0.3562792796168779
1101	42-43	0.36986349601372837
1101	44-45	0.2669002872563695
1101	46-47	0.20473994349566738
1101	48-49	0.33069186142455465
1101	50-51	0.378427488596067
1101	52-53	0.44696366496001616
1101	54-55	0.5084407729357068
1101	56-57	0.48352103437394334
1101	58-59	0.5471868648145914
1101	60-61	0.4755166463442251
1101	62-63	0.5347410054427968
1101	64-65	0.5401822057686658
1101	66-67	0.5022392838562482
1101	68-69	0.48578428012852726
1101	70-71	0.4111774427654922
1101	72-73	0.3595214770256874
1101	74-75	0.46126377120111783
1101	76-77	0.3081059422444383
1101	78-79	0.35583201641949813
1101	80-81	0.41704164730092685
1101	82-83	0.4204421543081942
1101	84-85	0.4524321017242201
1101	86-87	0.37023030176223415
1101	88-89	0.4582320289171271
1101	90-91	0.38992387543367
1101	92-93	0.3814509165640487
1101	94-95	0.393650756773404
1101	96-97	0.3769264241254575
1101	98-99	0.40340607491697256
1101	100-101	0.4415854105520829
1102	1	0.17225005344939603
1102	2	0.1417620032430591
1102	3	0.11302268013042038
1102	4	0.16275967933886193
1102	5	0.17541894336572028
1102	6	0.2033917532179359
1102	7	0.2891299692357592
1102	8	0.19364120765675352
1102	9	0.18342024042640048
1102	10-11	0.19927233176100856
1102	12-13	0.22661659245948584
1102	14-15	0.3380849133324162
1102	16-17	0.3409089817597888
1102	18-19	0.31571545952554914
1102	20-21	0.3815779990724124
1102	22-23	0.40953033567330266
1102	24-25	0.41072230589598746
1102	26-27	0.36288135310743996
1102	28-29	0.34232176954488125
1102	30-31	0.4106580488501379
1102	32-33	0.4389794195603116
1102	34-35	0.41765184210171924
1102	36-37	0.40203015128248865
1102	38-39	0.41438579952505705
1102	40-41	0.37426392430008804
1102	42-43	0.37204823486930394
1102	44-45	0.3113877262406035
1102	46-47	0.2611141902310763
1102	48-49	0.29476678085782737
1102	50-51	0.21834985451382494
1102	52-53	0.3158879086084312
1102	54-55	0.37962447688614276
1102	56-57	0.3981597493918301
1102	58-59	0.39122822367114196
1102	60-61	0.4801817465838667
1102	62-63	0.521885367563172
1102	64-65	0.5337548989458796
1102	66-67	0.5501091247655481
1102	68-69	0.5263147218887738
1102	70-71	0.575594780313395
1102	72-73	0.3893456241388549
1102	74-75	0.4529126193713253
1102	76-77	0.3790326730189477
1102	78-79	0.3777717051361904
1102	80-81	0.5453988006658221
1102	82-83	0.5750110051309356
1102	84-85	0.5220378325947692
1102	86-87	0.5034981095240809
1102	88-89	0.5651539169225153
1102	90-91	0.5394677625762263
1102	92-93	0.5636827330274059
1102	94-95	0.5191068982141225
1102	96-97	0.618335863542633
1102	98-99	0.7064842412198047
1102	100-101	0.6078523718678603
1103	1	0.052902736451439836
1103	2	0.03939315800759147
1103	3	0.03119037518453993
1103	4	0.07409971982365704
1103	5	0.08953011318120474
1103	6	0.07131716895806761
1103	7	0.11298908864186785
1103	8	0.10132955321191162
1103	9	0.1476141091436176
1103	10-11	0.10630303803827701
1103	12-13	0.1167693051673524
1103	14-15	0.1871987160036781
1103	16-17	0.17493589311759905
1103	18-19	0.14822339522516614
1103	20-21	0.2091802295556775
1103	22-23	0.1551311767018646
1103	24-25	0.15048449306532774
1103	26-27	0.1521052459288157
1103	28-29	0.12365704118346343
1103	30-31	0.12953898435736733
1103	32-33	0.10672146370316682
1103	34-35	0.1750883777232417
1103	36-37	0.12973141887258066
1103	38-39	0.1036762387045016
1103	40-41	0.1348525488071104
1103	42-43	0.06528954909754248
1103	44-45	0.03141021425924606
1103	46-47	-0.05842259866800248
1103	48-49	-0.08384937317113383
1103	50-51	0.0025912136544548048
1103	52-53	0.10607228985018935
1103	54-55	0.08402293741700362
1103	56-57	0.10966417980380783
1103	58-59	0.1465750957889682
1103	60-61	0.10599631396799936
1103	62-63	0.13353441054043458
1103	64-65	0.16705922489236258
1103	66-67	0.10671976765623725
1103	68-69	0.0925468258959441
1103	70-71	0.0969040257570839
1103	72-73	-0.029231719580202764
1103	74-75	-0.001869320405056385
1103	76-77	-0.0063590619711177965
1103	78-79	0.03179902848553695
1103	80-81	0.12365606232607718
1103	82-83	0.10473836044941365
1103	84-85	0.07626948959996938
1103	86-87	0.04961782117951685
1103	88-89	0.10617014974264549
1103	90-91	0.05559944143643136
1103	92-93	-0.05119836586187887
1103	94-95	0.05275873813649312
1103	96-97	0.044943992047215886
1103	98-99	0.17260832564376472
1103	100-101	0.15267360290564014
1104	1	0.1721196527658293
1104	2	0.17141116663451683
1104	3	0.11741848632178531
1104	4	0.2103133420734622
1104	5	0.154088338538223
1104	6	0.11282877658683077
1104	7	0.16843821703215411
1104	8	0.15713404149612842
1104	9	0.2325013201815338
1104	10-11	0.13699535866494728
1104	12-13	0.1333150311263651
1104	14-15	0.2377686725107182
1104	16-17	0.2722060486557609
1104	18-19	0.21844387450737202
1104	20-21	0.20852785979664645
1104	22-23	0.2322797357109181
1104	24-25	0.23364626909556563
1104	26-27	0.26512547136373854
1104	28-29	0.25365786465851414
1104	30-31	0.18219289981688291
1104	32-33	0.21377901474753003
1104	34-35	0.25997263016815
1104	36-37	0.39394215748039585
1104	38-39	0.29983637945462505
1104	40-41	0.31478102646472195
1104	42-43	0.2519863835509497
1104	44-45	0.2877383570698768
1104	46-47	0.19860069022561788
1104	48-49	0.13543315816160373
1104	50-51	0.1419799403960056
1104	52-53	0.24747396931194743
1104	54-55	0.30817350481761707
1104	56-57	0.31816510510376617
1104	58-59	0.3316590450438355
1104	60-61	0.39810235224265256
1104	62-63	0.3003655667602274
1104	64-65	0.3276720349755138
1104	66-67	0.46561612534881647
1104	68-69	0.44335122624420364
1104	70-71	0.447370365762211
1104	72-73	0.24865482758232105
1104	74-75	0.2563896330781361
1104	76-77	0.26806893105251817
1104	78-79	0.3914948226647681
1104	80-81	0.4307529215659116
1104	82-83	0.4372926798061876
1104	84-85	0.5638249488368885
1104	86-87	0.5192729297738481
1104	88-89	0.49023246395738695
1104	90-91	0.577791765432611
1104	92-93	0.4569096993777251
1104	94-95	0.4943293203859511
1104	96-97	0.3393130663346895
1104	98-99	0.43266618586109473
1104	100-101	0.5560577484302804
1105	1	0.09845925075004658
1105	2	0.09015319615546957
1105	3	0.07653027122219314
1105	4	0.05502902297605061
1105	5	-0.012137378869518045
1105	6	0.1099094602314139
1105	7	0.022315063442064798
1105	8	0.09288504533183328
1105	9	0.08612696170695244
1105	10-11	0.06272600375492488
1105	12-13	0.11797548322108042
1105	14-15	0.17999696979377688
1105	16-17	0.18397925497533407
1105	18-19	0.1483976629941708
1105	20-21	0.2899792013217777
1105	22-23	0.21961331488628844
1105	24-25	0.24904922588238776
1105	26-27	0.2601964966415622
1105	28-29	0.25070365123382743
1105	30-31	0.15025918781273617
1105	32-33	0.21343262902290405
1105	34-35	0.20376921216129773
1105	36-37	0.20086078214464465
1105	38-39	0.21150221314426432
1105	40-41	0.22058760557118973
1105	42-43	0.2284822139202518
1105	44-45	0.2464294287675557
1105	46-47	0.287260138118107
1105	48-49	0.33285513861599725
1105	50-51	0.4588782973671428
1105	52-53	0.43896481763751893
1105	54-55	0.5281184858937209
1105	56-57	0.4494414558634148
1105	58-59	0.4379567526555448
1105	60-61	0.47640236688052795
1105	62-63	0.38601723065789173
1105	64-65	0.40202119595273444
1105	66-67	0.43241955997662984
1105	68-69	0.4246966882478347
1105	70-71	0.4619337287702052
1105	72-73	0.3931360441624392
1105	74-75	0.476003718947382
1105	76-77	0.4226343010760374
1105	78-79	0.42797832929512936
1105	80-81	0.42856104223932334
1105	82-83	0.37892211328690095
1105	84-85	0.3888790151807484
1105	86-87	0.41835814814693606
1105	88-89	0.4208997229289828
1105	90-91	0.47252922179776746
1105	92-93	0.4026414000606451
1105	94-95	0.4291457618015855
1105	96-97	0.4876781974762956
1105	98-99	0.4019430877205039
1105	100-101	0.5435672018900952
1106	1	0.037761921094769946
1106	2	-0.03268745680396634
1106	3	-0.018611315221537694
1106	4	-0.048173430730258815
1106	5	-0.02898263148575353
1106	6	-0.022211823919370488
1106	7	-0.004420702329895221
1106	8	-0.05894220273478368
1106	9	-0.01576294260934219
1106	10-11	-0.04071883893723083
1106	12-13	-0.09194432511960571
1106	14-15	-0.02973414002459407
1106	16-17	0.011271629261464966
1106	18-19	0.004814246779439202
1106	20-21	0.05267517151366974
1106	22-23	0.03607324720503158
1106	24-25	0.03835481532026819
1106	26-27	0.021357857856408202
1106	28-29	0.10299701592656163
1106	30-31	0.09868663062457017
1106	32-33	0.013794283815123265
1106	34-35	-0.08230400286536366
1106	36-37	0.025540046783376624
1106	38-39	-0.04207576104678168
1106	40-41	0.14118305887507887
1106	42-43	0.10134058994533035
1106	44-45	0.11903655427396842
1106	46-47	-0.04572863796867921
1106	48-49	-0.015029874134178556
1106	50-51	-0.05439262992450722
1106	52-53	0.06322957104712401
1106	54-55	0.020070129030408168
1106	56-57	0.09229562005406677
1106	58-59	0.20059798985062827
1106	60-61	0.1313202048333224
1106	62-63	0.18243967348015389
1106	64-65	0.22097330405926385
1106	66-67	0.24953524572374164
1106	68-69	0.2666262296791473
1106	70-71	0.2663821139904172
1106	72-73	-0.02497609488647612
1106	74-75	0.09719664806971196
1106	76-77	0.1369468233601836
1106	78-79	0.26084512898904677
1106	80-81	0.27352748309328945
1106	82-83	0.34108024056352804
1106	84-85	0.36135822185462985
1106	86-87	0.3818382076130362
1106	88-89	0.3855620151260375
1106	90-91	0.3914055377353911
1106	92-93	0.42034987022646675
1106	94-95	0.4988135689996511
1106	96-97	0.6274871219781204
1106	98-99	0.6050546325254587
1106	100-101	0.6687415655799107
1107	1	-0.09982162871724753
1107	2	-0.054051510015707294
1107	3	-0.04147760263946765
1107	4	-0.10330487074282502
1107	5	-0.07370329177314972
1107	6	-0.058651416318319605
1107	7	-0.07168393675895857
1107	8	-0.14537985869991843
1107	9	-0.13740481926947012
1107	10-11	-0.08769193712173262
1107	12-13	-0.10388430297849283
1107	14-15	-0.05868872151739879
1107	16-17	-8.689339119527517E-4
1107	18-19	-0.006714476379947598
1107	20-21	0.02767891650361065
1107	22-23	-0.05285926624953419
1107	24-25	-0.014997736205309309
1107	26-27	-0.038397238070139394
1107	28-29	-0.023848387235304358
1107	30-31	-0.003576037488940642
1107	32-33	-0.037547262920526236
1107	34-35	0.05736797255288906
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2309	58-59	-5.391738555076131E-4
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2312	56-57	-0.6520675627511849
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2312	60-61	-0.6258437759003286
2312	62-63	-0.6172094864082425
2312	64-65	-0.713383143762222
2312	66-67	-0.599099875186532
2312	68-69	-0.6385605138666435
2312	70-71	-0.595263676017801
2312	72-73	-0.4934333238586106
2312	74-75	-0.6205291664423811
2312	76-77	-0.5311008732216322
2312	78-79	-0.6299345409131583
2312	80-81	-0.5732171553401884
2312	82-83	-0.6633963144683932
2312	84-85	-1.6774801845096903
2312	86-87	-1.0914159060708641
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2312	90-91	-0.6462458874480745
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2312	96-97	-0.5289725812646431
2312	98-99	-0.5691820245343067
2312	100-101	-0.6254128804945438
2313	1	-0.246066300809634
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2313	22-23	-0.46419035549168797
2313	24-25	-0.458658545597622
2313	26-27	-1.752153093147946
2313	28-29	-0.8369566757462508
2313	30-31	-0.6404655782771869
2313	32-33	-0.49229767108980127
2313	34-35	-0.4545488027780209
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2313	40-41	-0.44338134582940114
2313	42-43	-0.5837424756964893
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2313	48-49	-0.4130844505603477
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2313	54-55	-0.5227821524335994
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2313	60-61	-0.5791694762972739
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2313	64-65	-0.6275056414114886
2313	66-67	-0.5485195797334441
2313	68-69	-0.46382204230472013
2313	70-71	-0.5236498575538135
2313	72-73	-0.2672749957779885
2313	74-75	-0.43605378822253726
2313	76-77	-0.4096078762690354
2313	78-79	-0.5012768259421279
2313	80-81	-0.5190416770267277
2313	82-83	-0.5567975238406113
2313	84-85	-0.550759237057104
2313	86-87	-0.6574981260729125
2313	88-89	-0.7294058797279526
2313	90-91	-0.6408711031484877
2313	92-93	-0.7019045375720552
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2313	96-97	-0.6200922797107289
2313	98-99	-0.5674262689829774
2313	100-101	-0.665770609916688
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2314	2	-0.3910551932191879
2314	3	-0.30979742307963676
2314	4	-0.3327628215689842
2314	5	-0.3851698631121465
2314	6	-0.43799623390158615
2314	7	-0.41058999309409927
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2314	9	-0.4503497704677528
2314	10-11	-0.41346193761985717
2314	12-13	-0.42960120908757204
2314	14-15	-0.6562221472482577
2314	16-17	-0.6030717943282085
2314	18-19	-0.6160292891870611
2314	20-21	-0.6462485739074637
2314	22-23	-0.7517770432003559
2314	24-25	-0.6700766012980921
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2314	54-55	-0.6794115574894946
2314	56-57	-0.631560994998118
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2314	60-61	-0.7703737280008269
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2314	80-81	-0.7135905835520902
2314	82-83	-0.7371291036683836
2314	84-85	-0.7082986542272458
2314	86-87	-0.7946160865619021
2314	88-89	-0.6959870896052252
2314	90-91	-0.797842957006555
2314	92-93	-0.764350320222686
2314	94-95	-0.7840398955582728
2314	96-97	-0.8309078214588759
2314	98-99	-0.952362041041539
2314	100-101	-0.9595957305152929
2315	1	-0.4204692751612207
2315	2	-0.3916373062825116
2315	3	-0.513462089870842
2315	4	-0.4724123333511727
2315	5	-0.3981190153384091
2315	6	-0.417404047636758
2315	7	-0.465947648002512
2315	8	-0.5112200317627824
2315	9	-0.49269421334561514
2315	10-11	-0.5688371684302709
2315	12-13	-0.5767701204996527
2315	14-15	-0.8250176283155923
2315	16-17	-0.7792638515003674
2315	18-19	-0.7546584813412309
2315	20-21	-0.7316796968971246
2315	22-23	-0.7502137103025319
2315	24-25	-0.726616473813003
2315	26-27	-0.8059464651976853
2315	28-29	-0.748660198834358
2315	30-31	-0.7456004421485929
2315	32-33	-0.6759165297034571
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2315	36-37	-0.7697657805373765
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2315	48-49	-0.7284217970235147
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2315	56-57	-0.9257287856677081
2315	58-59	-1.1046041224420762
2315	60-61	-0.9933127178283883
2315	62-63	-1.0159778637767332
2315	64-65	-0.9682201413218365
2315	66-67	-0.9109375694780724
2315	68-69	-0.8264638249848062
2315	70-71	-0.8927697283444331
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2315	80-81	-0.8334516974365371
2315	82-83	-0.8480834721061221
2315	84-85	-0.8201260148791789
2315	86-87	-0.9296482497530363
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2315	90-91	-0.872368227737784
2315	92-93	-0.9473263562810601
2315	94-95	-0.9740691238575856
2315	96-97	-1.0016936416590205
2315	98-99	-0.9508461194941233
2315	100-101	-0.9328352033963547
2316	1	-0.5869970637692603
2316	2	-1.1345704703133848
2316	3	-0.7452929519139708
2316	4	-0.6102023243025485
2316	5	-0.5740906586804044
2316	6	-0.7763745897680181
2316	7	-0.924017030937641
2316	8	-0.770949892568666
2316	9	-0.777976499646428
2316	10-11	-0.6403065762500901
2316	12-13	-0.7391232852932461
2316	14-15	-0.9835876959098258
2316	16-17	-1.0300404937040568
2316	18-19	-1.072939820516062
2316	20-21	-1.2165015917026878
2316	22-23	-0.9891699742798821
2316	24-25	-0.9663937206342723
2316	26-27	-0.9673650921847425
2316	28-29	-1.000091150951036
2316	30-31	-1.0616060182558158
2316	32-33	-1.0560718961150855
2316	34-35	-0.9772512704351328
2316	36-37	-1.0884788872124034
2316	38-39	-1.055619998550192
2316	40-41	-0.9570906578049971
2316	42-43	-1.0112140223701616
2316	44-45	-0.9559604400503687
2316	46-47	-0.8361566503272684
2316	48-49	-0.8922726243788617
2316	50-51	-0.9500679753803993
2316	52-53	-1.0349931820799654
2316	54-55	-0.9984560892660994
2316	56-57	-1.030453481057279
2316	58-59	-1.0369135744908178
2316	60-61	-1.1500622602654502
2316	62-63	-1.1294782638743115
2316	64-65	-1.1145986102443501
2316	66-67	-1.137352943901
2316	68-69	-1.164413505525836
2316	70-71	-1.0537375198426702
2316	72-73	-0.7362680506487251
2316	74-75	-0.8925971235626093
2316	76-77	-0.9250357334315495
2316	78-79	-0.8681520312085311
2316	80-81	-0.9003404268354736
2316	82-83	-0.8232395475827765
2316	84-85	-0.9016754823870237
2316	86-87	-0.9934023586569509
2316	88-89	-0.9265635054808108
2316	90-91	-0.9162717900507893
2316	92-93	-0.9694096460258308
2316	94-95	-0.8810280289577186
2316	96-97	-0.8943304924483506
2316	98-99	-0.8754216999107669
2316	100-101	-0.8898202698869504
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	122541.0
3	19166.0
4	13829.0
5	11726.0
6	11771.0
7	12498.0
8	12868.0
9	13214.0
10	13862.0
11	14126.0
12	14528.0
13	15404.0
14	15866.0
15	18145.0
16	20877.0
17	25342.0
18	22571.0
19	18582.0
20	19081.0
21	21012.0
22	23330.0
23	27087.0
24	31216.0
25	36708.0
26	41566.0
27	48982.0
28	59066.0
29	72123.0
30	89516.0
31	112426.0
32	145921.0
33	193501.0
34	267233.0
35	391181.0
36	622627.0
37	1240791.0
38	1402234.0
39	695.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.28636479024287	24.259042646422984	13.62138227866546	22.833210284668684
2	13.591745814858747	22.281672770981114	44.22020315417871	19.906378259981427
3	16.679759803402693	31.328910466022215	31.12662960917988	20.864700121395217
4	10.548960827256687	19.25777217216866	44.61938132359468	25.573885676979973
5	11.334076785357695	39.42040876891936	37.46817012577843	11.777344319944522
6	24.504139828791967	42.75732127558451	21.428391604230384	11.310147291393138
7	22.95251460364372	33.327185702199344	28.29868408906602	15.421615605090924
8	22.033154486219516	38.546391029010465	22.963023429149917	16.457431055620106
9	25.25293655873537	16.127061045786437	24.454857060900835	34.16514533457736
10-11	21.85947455440916	28.2096720575785	31.99750648791856	17.933346900093778
12-13	22.351057730709037	26.183903512469282	31.312607210168743	20.152431546652945
14-15	20.09411903377076	27.97996163104946	28.4193916832434	23.50652765193638
16-17	19.018118751866787	29.98070176308601	30.36707657459537	20.634102910451833
18-19	18.790928847925617	30.777177603770657	31.194075013877985	19.237818534425745
20-21	19.474886717806445	29.421271676719506	30.610933775988247	20.4929078294858
22-23	19.619834177980977	30.18156618500263	29.830178524156565	20.368421112859828
24-25	19.55515427473737	30.02415885990474	30.57371713631208	19.846969729045806
26-27	19.7518334181414	29.689701656160384	29.986761931426763	20.57170299427145
28-29	19.467093834847798	30.615746607232357	29.69209522712414	20.225064330795703
30-31	20.68742404465049	29.945098538834593	29.26808032938588	20.099397087129034
32-33	20.387407566201787	30.17024678765612	29.343577944206718	20.098767701935376
34-35	20.67261442032098	29.272991441124258	30.074208328787773	19.98018580976699
36-37	20.902263726891075	29.77488798850781	29.981393085002097	19.341455199599025
38-39	21.314444275760735	29.149784521396427	30.57692498415094	18.958846218691903
40-41	20.375629207531084	29.199854478250646	30.6611751119664	19.76334120225187
42-43	20.925562510686436	29.258766311448625	29.763876585943557	20.051794591921382
44-45	20.202381036390328	30.213988498199278	29.818236226533557	19.765394238876834
46-47	20.96439567898678	29.33631314806094	29.1893050685261	20.509986104426183
48-49	20.978027462435836	28.35602692643843	30.09773083245958	20.568214778666153
50-51	20.95192858333062	28.987022115647964	28.94222389914943	21.118825401871987
52-53	19.81109604278202	29.44235177455015	29.747573960204747	20.998978222463087
54-55	20.55477653481068	29.85024939614994	29.02426519375927	20.570708875280108
56-57	20.82016341397598	28.901930282991405	29.598157194958553	20.67974910807406
58-59	20.430878338855145	28.49436985573458	30.666821631347634	20.407930174062646
60-61	20.466413333964514	30.179833703195253	28.87188528207769	20.48186768076254
62-63	20.836873179473546	29.3441801486831	28.40026049187445	21.4186861799689
64-65	19.758137853677848	29.289407426744734	28.94304721633337	22.00940750324405
66-67	20.441468413797615	28.078465425794086	29.60204030606503	21.878025854343267
68-69	19.62086441582536	28.91768450207518	29.07660801679991	22.384843065299545
70-71	19.879894792021503	28.622203220122866	29.782173670413663	21.715728317441968
72-73	20.51605544840864	28.424315249466385	29.86351390273304	21.196115399391932
74-75	20.366242267265182	29.295372969303045	28.97070119398892	21.36768356944286
76-77	19.930374906784674	29.188536893015733	29.90461872880068	20.97646947139891
78-79	19.732007780419277	28.936243403613748	29.542344267664493	21.78940454830248
80-81	19.77422960590967	29.07328846383449	30.176587550627428	20.97589437962841
82-83	20.605073161381444	28.087755206864223	30.165431504325102	21.14174012742923
84-85	21.347298665735252	28.06300926299633	29.243856708757516	21.345835362510897
86-87	20.156812380379694	29.240751139754384	29.32888902189994	21.273547457965986
88-89	20.905723080900774	28.021733513935988	29.596212316359093	21.476331088804145
90-91	20.743273036480964	27.51136130326195	30.19710712430193	21.548258535955156
92-93	20.13261106616483	28.552605116942743	29.6935959453528	21.621187871539625
94-95	19.52452885212496	27.985463789377064	29.973290209853353	22.51671714864462
96-97	19.97848386920546	28.02048158388063	29.300017076900687	22.70101747001323
98-99	19.489849417836012	28.75428837130578	29.610496440975382	22.145365769882822
100-101	20.111048635283087	27.35473088523812	29.397639521646347	23.136580957832447
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	33.0
1	22.5
2	28.5
3	41.5
4	38.0
5	41.0
6	53.5
7	96.0
8	168.0
9	213.0
10	203.0
11	230.0
12	357.5
13	590.0
14	791.0
15	983.0
16	1367.0
17	1653.0
18	1758.0
19	1917.5
20	2131.0
21	2391.5
22	2663.0
23	3035.0
24	3431.5
25	4012.5
26	5377.0
27	8461.5
28	16073.0
29	34065.5
30	60213.5
31	90862.5
32	130802.0
33	183201.0
34	253370.0
35	306994.5
36	347793.5
37	406231.0
38	418602.0
39	361736.5
40	299760.5
41	248285.5
42	208464.0
43	182707.0
44	158103.0
45	140246.5
46	126980.0
47	118437.0
48	112653.0
49	105296.0
50	98625.0
51	94416.5
52	89049.0
53	84044.5
54	79468.5
55	71260.5
56	64065.5
57	54389.0
58	44207.5
59	40248.5
60	34706.0
61	27238.0
62	21394.5
63	17797.5
64	14250.0
65	10153.5
66	7714.5
67	6157.5
68	5003.5
69	4144.0
70	3545.0
71	2984.5
72	2539.5
73	2179.5
74	1846.0
75	1547.0
76	1291.0
77	1075.0
78	913.0
79	770.5
80	613.5
81	503.0
82	412.0
83	335.0
84	277.0
85	232.0
86	202.0
87	153.0
88	112.0
89	94.5
90	67.5
91	51.0
92	45.5
93	39.0
94	29.0
95	23.5
96	21.5
97	13.5
98	7.0
99	4.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.530960792735445E-4
2	0.003795383440532254
3	1.3350595016947627E-4
4	9.726862083776129E-4
5	0.001964444695350865
6	0.0
7	0.0
8	0.0
9	0.0
10-11	9.536139297819734E-5
12-13	1.144336715738368E-4
14-15	0.003251823500556529
16-17	0.004691780534527308
18-19	0.0037286304654475157
20-21	9.536139297819734E-6
22-23	0.0
24-25	1.9072278595639467E-5
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	1.8118664665857495E-4
42-43	9.536139297819734E-6
44-45	6.293851936561024E-4
46-47	0.7582470439875404
48-49	0.5461251614468383
50-51	0.4200097192331723
52-53	0.3527131842084585
54-55	0.34312936421414963
56-57	0.3085990038167444
58-59	0.30924746128899616
60-61	0.2843581377216866
62-63	0.27925630319735306
64-65	0.2747743177273778
66-67	0.26320698075912247
68-69	0.2423800525326842
70-71	0.2551966237489539
72-73	0.8385031923179914
74-75	0.5564718725849728
76-77	0.6517092957522984
78-79	0.36864807297511526
80-81	0.3203379912923605
82-83	0.3006077190851714
84-85	0.2920728744136228
86-87	0.2652095700116646
88-89	0.2680227311045214
90-91	0.27861738186439916
92-93	0.23413129204007008
94-95	0.20000144949317328
96-97	0.1539132882668105
98-99	0.1632873131965673
100-101	0.15827130392591413
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5243212.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.789229748021846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.80002344678206	30.178029838787158
2	12.028841378748641	9.331789836855858
3	4.320123513787989	5.027227905484612
4	1.9930287712946044	3.0923220361665638
5	1.028650576396983	1.9950281769148839
6	0.6187304342261145	1.4400046179174044
7	0.3828695197120082	1.0395849634536705
8	0.27355628589824643	0.8488830098178104
9	0.19099001178316233	0.6667519901971034
>10	1.0759482393477198	7.890291754141286
>50	0.11842530015086115	3.274244708598438
>100	0.13389181274480094	11.080335488794557
>500	0.01797897911673998	4.799638554317196
>1k	0.01501541085432276	11.398219375329251
>5k	0.0010866415749838834	2.829873672502637
>10k+	8.396775806693644E-4	5.107774070721565
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	24341	0.4642383332964603	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	21735	0.4145359752762238	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	20678	0.39437657680063287	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	20292	0.38701467726271604	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	17174	0.3275473126015122	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	16241	0.3097528766717806	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	16235	0.30963844300020676	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	15566	0.29687908861972395	No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC	15394	0.29359865670127394	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	15030	0.28665634729246114	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	13497	0.2574185442053459	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	13394	0.255454099509995	No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC	13335	0.2543288350728523	No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG	11546	0.22020852866525328	No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA	11132	0.21231260532665855	No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA	10506	0.20037335892578823	No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA	10502	0.2002970698114057	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	8901	0.16976235177978688	No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA	8510	0.16230509084889186	No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA	7845	0.1496220255827916	No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT	7801	0.14878284532458347	No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA	7726	0.1473524244299105	No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG	7485	0.1427560052883614	No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA	7429	0.1416879576870056	No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG	7301	0.13924670602676376	No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG	6938	0.13232346889654661	No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG	6796	0.12961520533596582	No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG	6687	0.1275363269690411	No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC	6643	0.126697146710833	No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT	6443	0.1228826909917051	No Hit
GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT	6325	0.12063216211741963	No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT	6111	0.11655069449795277	No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG	6035	0.11510120132468418	No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT	5657	0.10789188001553247	No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG	5584	0.10649960367805078	No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT	5577	0.10636609772788132	No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA	5529	0.10545062835529062	No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA	5309	0.10125472706424993	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.9072278595639467E-5	0.0
10-11	0.0	0.0	0.0	1.9072278595639467E-5	0.0
12-13	0.0	0.0	0.0	1.9072278595639467E-5	0.0
14-15	0.0	0.0	0.0	2.86084178934592E-5	0.0
16-17	0.0	0.0	0.0	6.675297508473813E-5	0.0
18-19	0.0	0.0	0.0	8.58252536803776E-5	0.0
20-21	0.0	0.0	0.0	1.1443367157383679E-4	0.0
22-23	0.0	0.0	0.0	3.3376487542369064E-4	0.0
24-25	0.0	0.0	0.0	8.487163975059563E-4	0.0
26-27	0.0	0.0	0.0	0.0015448545662467969	0.0
28-29	0.0	0.0	0.0	0.0035188354008954814	0.0
30-31	0.0	0.0	0.0	0.014084877742879746	0.0
32-33	0.0	0.0	0.0	0.04764255193190739	0.0
34-35	9.536139297819734E-6	0.0	0.0	0.1031238103666226	0.0
36-37	1.9072278595639467E-5	0.0	0.0	0.19182897811494176	0.0
38-39	1.9072278595639467E-5	0.0	0.0	0.31657121626972173	0.0
40-41	1.9072278595639467E-5	0.0	0.0	0.44684441521723706	1.9072278595639467E-5
42-43	1.9072278595639467E-5	0.0	0.0	0.5937581772394478	1.9072278595639467E-5
44-45	1.9072278595639467E-5	0.0	0.0	0.7630246497757482	1.9072278595639467E-5
46-47	1.9072278595639467E-5	9.536139297819734E-6	0.0	0.966859627266645	1.9072278595639467E-5
48-49	1.9072278595639467E-5	1.9072278595639467E-5	0.0	1.1765116497292119	1.9072278595639467E-5
50-51	3.8144557191278935E-5	1.9072278595639467E-5	0.0	1.3883569842302772	1.9072278595639467E-5
52-53	3.8144557191278935E-5	1.9072278595639467E-5	0.0	1.6014134084221656	3.8144557191278935E-5
54-55	3.8144557191278935E-5	1.9072278595639467E-5	0.0	1.8287454331428903	3.8144557191278935E-5
56-57	3.8144557191278935E-5	1.9072278595639467E-5	0.0	2.10215608295068	4.768069648909867E-5
58-59	3.8144557191278935E-5	1.9072278595639467E-5	0.0	2.418841732891975	5.72168357869184E-5
60-61	3.8144557191278935E-5	1.9072278595639467E-5	0.0	2.7373106408819634	5.72168357869184E-5
62-63	3.8144557191278935E-5	1.9072278595639467E-5	0.0	3.0562277474189488	7.628911438255787E-5
64-65	3.8144557191278935E-5	1.9072278595639467E-5	0.0	3.3936354280544063	7.628911438255787E-5
66-67	3.8144557191278935E-5	1.9072278595639467E-5	0.0	3.7600997251303205	7.628911438255787E-5
68-69	3.8144557191278935E-5	1.9072278595639467E-5	0.0	4.157394360556086	7.628911438255787E-5
70-71	3.8144557191278935E-5	1.9072278595639467E-5	0.0	4.56864990391386	7.628911438255787E-5
72-73	3.8144557191278935E-5	1.9072278595639467E-5	0.0	5.024467826210346	9.536139297819734E-5
74-75	3.8144557191278935E-5	1.9072278595639467E-5	0.0	5.456254677476325	1.144336715738368E-4
76-77	3.8144557191278935E-5	1.9072278595639467E-5	0.0	5.933948503322009	1.144336715738368E-4
78-79	3.8144557191278935E-5	1.9072278595639467E-5	0.0	6.467543177731512	1.144336715738368E-4
80-81	3.8144557191278935E-5	1.9072278595639467E-5	0.0	7.060557536105731	1.144336715738368E-4
82-83	3.8144557191278935E-5	1.9072278595639467E-5	0.0	7.6957788470120985	1.144336715738368E-4
84-85	3.8144557191278935E-5	2.86084178934592E-5	0.0	8.29779532088346	1.144336715738368E-4
86-87	3.8144557191278935E-5	3.8144557191278935E-5	0.0	8.94971441170031	1.144336715738368E-4
88-89	3.8144557191278935E-5	3.8144557191278935E-5	0.0	9.631491154658633	1.144336715738368E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCTGGG	4015	0.0	49.16832	3
TACCTGG	4100	0.0	48.14944	2
GTACCTG	5015	0.0	40.21404	1
CCTGGGG	3740	0.0	39.619373	4
CTGGGGG	3505	0.0	37.278286	5
TGGGGGG	9415	0.0	28.81117	6
GTACACG	705	0.0	28.20325	1
TATTCGC	875	0.0	27.593142	9
GTATCAA	9500	0.0	25.31507	1
GGGGGGA	9715	0.0	25.290136	7
CAACGCA	9530	0.0	23.993252	5
TATATCC	6255	0.0	23.916336	4
CATGGGG	9605	0.0	23.608753	4
GTACATG	14900	0.0	23.607044	1
GTACTAG	2080	0.0	23.442984	1
ATGGGGG	8620	0.0	23.285925	5
ATCAACG	9835	0.0	23.104776	3
AGGGCTA	7460	0.0	22.908848	5
ATATATC	6940	0.0	22.578932	3
TACATGG	15370	0.0	22.453947	2
>>END_MODULE