FastQCFastQC Report
Sat 18 Jun 2016
SRR1931052_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931052_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162135
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN929064.296956480515787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18650.061.7093541
GTATCAA35800.046.8638731
ATCAACG44150.036.4620633
CAACGCA45400.035.107635
TATCAAC46100.034.7252352
TCAACGC46550.034.684284
AACGCAG50650.033.0857286
ACGCAGA55150.029.0390347
CGCAGAG55850.028.8046658
GTACATG37950.024.6653121
GCAGAGT60300.024.283569
GTGGTAT9300.023.100091
TACATGG39100.022.8909132
ACATGGG37850.022.7275283
GTGTAGC8300.022.1856291
GAGTACT40350.021.83922412-13
CAGAGTA58400.021.12555710-11
AGAGTAC54600.020.4538510-11
TGGTATC9800.019.3097862
GTACTTT46750.018.70402514-15
Produced by FastQC (version 0.11.5)