FastQCFastQC Report
Sat 18 Jun 2016
SRR1931052_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931052_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162135
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60740.280926029133241No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56380.2607607758072461No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32130.14860311682665514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29300.064.386381
GTATCAA57600.042.156991
ACGCGAG702.0677512E-433.8898547
ATCAACG71750.033.375633
TCAACGC73150.032.8018154
CAACGCA74450.031.9294055
TATCAAC76850.031.2239972
AACGCAG77550.030.8963586
CGCGAGT856.4519525E-427.909298
CGGGCGT2600.027.3725746
ACGCAGA87950.027.027117
CGCAGAG88650.026.8672188
GGCGTCG2451.0913936E-1125.175328
TATCACG1551.4074303E-624.524452
ACGGGCG2653.274181E-1123.2763735
GCAGAGT100550.023.0740879
CGCGGAA953.3232573E-722.47744232-33
GTACATG61200.022.2844351
GAGTACT73450.021.219812-13
CAGAGTA97400.021.01936310-11
Produced by FastQC (version 0.11.5)