##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931052_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2162135 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.306395299090944 31.0 31.0 34.0 30.0 34.0 2 31.465130993208103 31.0 31.0 34.0 30.0 34.0 3 31.52738566278239 31.0 31.0 34.0 30.0 34.0 4 35.30674032842538 37.0 35.0 37.0 33.0 37.0 5 35.17846341694668 37.0 35.0 37.0 32.0 37.0 6 35.21011546457552 37.0 35.0 37.0 32.0 37.0 7 35.08902311835293 37.0 35.0 37.0 32.0 37.0 8 35.093953430289965 37.0 35.0 37.0 32.0 37.0 9 36.59592254877702 39.0 35.0 39.0 32.0 39.0 10-11 36.53882574399841 38.0 35.0 39.0 32.0 39.0 12-13 36.41660488359885 38.0 35.0 39.0 32.0 39.0 14-15 37.465183256364654 39.0 36.0 41.0 32.0 41.0 16-17 37.41638288080994 39.0 36.0 41.0 32.0 41.0 18-19 37.44872036204954 39.0 36.0 41.0 32.0 41.0 20-21 37.43819604233778 39.0 36.0 41.0 32.0 41.0 22-23 37.33432880000555 39.0 36.0 40.5 31.5 41.0 24-25 37.20790468680263 39.0 36.0 40.0 31.0 41.0 26-27 36.94839290793591 39.0 36.0 40.0 31.0 41.0 28-29 36.762374227326234 39.0 35.5 40.0 30.0 41.0 30-31 36.5263917377962 38.0 35.0 40.0 30.0 41.0 32-33 36.353529959970125 38.0 35.0 40.0 30.0 41.0 34-35 36.42287808115589 38.0 35.0 40.0 30.0 41.0 36-37 36.486771640068724 38.0 35.0 40.0 30.0 41.0 38-39 36.46030705760741 38.5 35.0 40.0 30.0 41.0 40-41 36.34233755061548 38.5 35.0 40.0 30.0 41.0 42-43 36.19571904622052 38.0 35.0 40.0 30.0 41.0 44-45 36.0147308100558 38.0 35.0 40.0 29.5 41.0 46-47 35.75847414708147 38.0 35.0 40.0 28.5 41.0 48-49 35.52426698610401 38.0 34.5 40.0 27.5 41.0 50-51 35.26055264819264 38.0 34.0 40.0 26.5 41.0 52-53 35.04676627500133 38.0 34.0 40.0 26.0 41.0 54-55 34.89793699283347 38.0 34.0 40.0 26.0 41.0 56-57 34.721037770536995 38.0 33.5 40.0 26.0 41.0 58-59 34.51055392008362 38.0 33.0 40.0 26.0 41.0 60-61 34.205659914852674 37.0 33.0 40.0 25.0 41.0 62-63 33.94978828796537 37.0 33.0 40.0 24.0 41.0 64-65 33.63651737749956 36.5 32.0 39.5 23.0 41.0 66-67 33.31831245505022 36.0 32.0 39.0 23.0 41.0 68-69 32.96846404132952 36.0 31.5 39.0 22.0 40.0 70-71 32.60247810613121 35.0 31.0 38.5 22.0 40.0 72-73 32.18112143783806 35.0 31.0 38.0 21.5 40.0 74-75 31.665956566079362 35.0 30.5 37.0 20.0 39.5 76-77 30.287590969111548 33.5 28.5 36.0 18.5 38.5 78-79 31.031274874140607 34.0 30.0 36.0 20.0 39.0 80-81 30.998783147213288 34.0 30.0 36.0 20.0 38.5 82-83 30.730343387438804 34.0 30.0 36.0 19.0 37.0 84-85 30.386240220892773 34.0 30.0 35.0 18.0 37.0 86-87 30.03551489615588 34.0 30.0 35.0 17.0 36.5 88-89 29.681445191905226 34.0 29.0 35.0 11.0 36.0 90-91 29.335794249665263 34.0 29.0 35.0 7.0 36.0 92-93 29.00887271146344 34.0 29.0 35.0 2.0 35.5 94-95 28.620842824337984 34.0 29.0 35.0 2.0 35.0 96-97 28.1847537272187 34.0 29.0 35.0 2.0 35.0 98-99 27.675111174834136 33.0 28.0 35.0 2.0 35.0 100-101 26.528190885397997 32.5 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3058188736953156 1101 2 0.39410978044903544 1101 3 0.35548768423193167 1101 4 0.1969475999653909 1101 5 0.26880603574980455 1101 6 0.24521985620659592 1101 7 0.36495059084450077 1101 8 0.38222755014278675 1101 9 0.5578845779368748 1101 10-11 0.4408731693880128 1101 12-13 0.4288705905573025 1101 14-15 0.4680199386872488 1101 16-17 0.6603826261034698 1101 18-19 0.49815696170993107 1101 20-21 0.4937060541441909 1101 22-23 0.49939941493408924 1101 24-25 0.33752346436084224 1101 26-27 0.3469298212560403 1101 28-29 0.5432032159707987 1101 30-31 0.43099836197836083 1101 32-33 0.5058322964057993 1101 34-35 0.5219785634064493 1101 36-37 0.6257466608114797 1101 38-39 0.6961881824345824 1101 40-41 0.7498220934102164 1101 42-43 0.7469601988631354 1101 44-45 0.599572349667632 1101 46-47 0.42384600108709947 1101 48-49 0.5217254239046198 1101 50-51 0.6400772841578757 1101 52-53 0.462419689319276 1101 54-55 0.5522770323310695 1101 56-57 0.6187532772006321 1101 58-59 0.5710205275353033 1101 60-61 0.39087406923325574 1101 62-63 0.22349217754477735 1101 64-65 0.4445007537246539 1101 66-67 0.35162934617964936 1101 68-69 0.3575406385919706 1101 70-71 0.3226227149835239 1101 72-73 0.26265672087709646 1101 74-75 0.30501542016803285 1101 76-77 0.1469372578920023 1101 78-79 0.43835074271672525 1101 80-81 0.33558679071711595 1101 82-83 0.5357473117266593 1101 84-85 0.45582449150406035 1101 86-87 0.4416185754575821 1101 88-89 0.337803824566123 1101 90-91 0.30145257810946546 1101 92-93 0.1469269865934777 1101 94-95 0.23195071701101355 1101 96-97 0.3978531047025946 1101 98-99 0.5609028453805358 1101 100-101 0.8426972043875232 1102 1 0.31013877094477493 1102 2 0.32772222006767393 1102 3 0.25882368681678614 1102 4 0.21273136109719104 1102 5 0.3612261722359591 1102 6 0.2691592309352231 1102 7 0.3444299776278541 1102 8 0.30172155811683865 1102 9 0.5371739696827049 1102 10-11 0.4328533486153958 1102 12-13 0.33493250480710657 1102 14-15 0.43514438103169795 1102 16-17 0.5076119640952541 1102 18-19 0.49535673746982667 1102 20-21 0.42346573148972055 1102 22-23 0.5060666702278525 1102 24-25 0.566216966932771 1102 26-27 0.5137926756748286 1102 28-29 0.40928266645611444 1102 30-31 0.3933776208023616 1102 32-33 0.4995127588445314 1102 34-35 0.5058136630360508 1102 36-37 0.5992882238539181 1102 38-39 0.40808800065606476 1102 40-41 0.487825352821055 1102 42-43 0.5844676133620084 1102 44-45 0.50337309313813 1102 46-47 0.3806824611348034 1102 48-49 0.42188504271940985 1102 50-51 0.30929008223892396 1102 52-53 0.2678655369987979 1102 54-55 0.36254275916072487 1102 56-57 0.2238916853295052 1102 58-59 0.35645771435232376 1102 60-61 0.3069034449176016 1102 62-63 0.25827898568369534 1102 64-65 0.16703732537528282 1102 66-67 0.14075582675715737 1102 68-69 0.18051314542901764 1102 70-71 0.17734622719501658 1102 72-73 0.2670114760870703 1102 74-75 0.33868734815224855 1102 76-77 0.2796489831331286 1102 78-79 0.4345246984957605 1102 80-81 0.3732885479222219 1102 82-83 0.23313013008469952 1102 84-85 0.30605238020910264 1102 86-87 0.25505800467571405 1102 88-89 0.2979177947748255 1102 90-91 0.2889124268144272 1102 92-93 0.3695426590006612 1102 94-95 0.43406579079976026 1102 96-97 0.4396791140655978 1102 98-99 0.5486656902038156 1102 100-101 0.8601572120601055 1103 1 0.3549406251721585 1103 2 0.4385408972914391 1103 3 0.41418384875671066 1103 4 0.43260140507028666 1103 5 0.3527245168119393 1103 6 0.33747954261620805 1103 7 0.3991885830689341 1103 8 0.41935158562146313 1103 9 0.6027198021503608 1103 10-11 0.5079362151197842 1103 12-13 0.46362918957510857 1103 14-15 0.452117575933336 1103 16-17 0.5372576823891677 1103 18-19 0.563001632340395 1103 20-21 0.5345311498059289 1103 22-23 0.5334977228183035 1103 24-25 0.579419569103024 1103 26-27 0.5406201703902482 1103 28-29 0.675720254969363 1103 30-31 0.5389648722266784 1103 32-33 0.5437595647434392 1103 34-35 0.5959461160180055 1103 36-37 0.5172212215914129 1103 38-39 0.5080181430968622 1103 40-41 0.4320608175882583 1103 42-43 0.5031292073342541 1103 44-45 0.6214858335177098 1103 46-47 0.6322606892116269 1103 48-49 0.45612944696690505 1103 50-51 0.6732771285698291 1103 52-53 0.6695172441726811 1103 54-55 0.6689803286132445 1103 56-57 0.6921918879450217 1103 58-59 0.7967775342665888 1103 60-61 0.702531867509137 1103 62-63 0.645696047701577 1103 64-65 0.6287308899025135 1103 66-67 0.6084079647034457 1103 68-69 0.4441028709355095 1103 70-71 0.5115196302397749 1103 72-73 0.3968904976619001 1103 74-75 0.5517791122033415 1103 76-77 0.6442978178556729 1103 78-79 0.7593871277089939 1103 80-81 0.6409041845790497 1103 82-83 0.5477285491881219 1103 84-85 0.4984770760475712 1103 86-87 0.3597585702074433 1103 88-89 0.556629594698201 1103 90-91 0.38676828579555433 1103 92-93 0.4253708570568513 1103 94-95 0.43888310327557534 1103 96-97 0.8012940530998662 1103 98-99 0.8611980219595559 1103 100-101 1.093061456693082 1104 1 0.27677468630752955 1104 2 0.3119033427062874 1104 3 0.284489525612603 1104 4 0.09592018236286748 1104 5 0.21953674388618083 1104 6 0.21564548148083418 1104 7 0.17779120315626784 1104 8 0.2835437253594506 1104 9 0.35817831743420214 1104 10-11 0.39636416271804364 1104 12-13 0.4527121590074259 1104 14-15 0.4518992353219886 1104 16-17 0.37677733304418837 1104 18-19 0.47217193801724733 1104 20-21 0.5126970886705564 1104 22-23 0.4952881158314 1104 24-25 0.4667558136445038 1104 26-27 0.4807948428793267 1104 28-29 0.5145848837903273 1104 30-31 0.45599543991226454 1104 32-33 0.46581196649016476 1104 34-35 0.44332602868176707 1104 36-37 0.5567408722464364 1104 38-39 0.654961374537919 1104 40-41 0.5656852717367258 1104 42-43 0.5219064999106706 1104 44-45 0.6852412920329911 1104 46-47 0.5582432219627265 1104 48-49 0.4146447308096981 1104 50-51 0.34838629887550354 1104 52-53 0.16296702583206724 1104 54-55 0.35173142031630533 1104 56-57 0.3852049883817017 1104 58-59 0.38869893164650904 1104 60-61 0.4285144002602337 1104 62-63 0.28019386429546245 1104 64-65 0.08353438335229413 1104 66-67 0.28460883806589266 1104 68-69 0.09322950849009004 1104 70-71 0.19776417172449356 1104 72-73 0.245798794605129 1104 74-75 0.2572376274871857 1104 76-77 0.33774759951506184 1104 78-79 0.49868843775266214 1104 80-81 0.4727819138366911 1104 82-83 0.4527503832492563 1104 84-85 0.42118449962836024 1104 86-87 0.3407629370196723 1104 88-89 0.45335448552789614 1104 90-91 0.5171176307737184 1104 92-93 0.5004800273625278 1104 94-95 0.5591887801314712 1104 96-97 0.7871019133618766 1104 98-99 0.6740801180294262 1104 100-101 0.9958998846406821 1105 1 0.350022727223962 1105 2 0.32778014476407336 1105 3 0.3393610635679778 1105 4 0.14611833394508267 1105 5 0.1613527096695151 1105 6 0.25871054997398346 1105 7 0.357326257292776 1105 8 0.35275994238403996 1105 9 0.378998440662059 1105 10-11 0.42095720423266414 1105 12-13 0.4020586326570026 1105 14-15 0.5344218918991217 1105 16-17 0.5630523450080602 1105 18-19 0.5613642646906882 1105 20-21 0.5078872394153109 1105 22-23 0.4967473339925519 1105 24-25 0.5788333395163718 1105 26-27 0.5717664212127644 1105 28-29 0.6062460670169756 1105 30-31 0.592963982413913 1105 32-33 0.5788649215658381 1105 34-35 0.6808353754545067 1105 36-37 0.4569528570523218 1105 38-39 0.5552193454657157 1105 40-41 0.38017781675078055 1105 42-43 0.3273711840645177 1105 44-45 0.40126782939017147 1105 46-47 0.4285692747832073 1105 48-49 0.3283979460995212 1105 50-51 0.36157384747873067 1105 52-53 0.31679172523985244 1105 54-55 0.3542415896050528 1105 56-57 0.48873077799706266 1105 58-59 0.48982353534334067 1105 60-61 0.4612232445504745 1105 62-63 0.311434704458172 1105 64-65 0.2956666813124542 1105 66-67 0.3363667790840168 1105 68-69 0.25802974926295974 1105 70-71 0.28626352866653093 1105 72-73 0.534334603960879 1105 74-75 0.4644980136210606 1105 76-77 0.4707609616613766 1105 78-79 0.5117621426638301 1105 80-81 0.43737615173822775 1105 82-83 0.5236407942081591 1105 84-85 0.5370605539443254 1105 86-87 0.4909769781155582 1105 88-89 0.5775620661344654 1105 90-91 0.5046460449628682 1105 92-93 0.5803321240305905 1105 94-95 0.7763500157000571 1105 96-97 0.6608180406574427 1105 98-99 0.7119975449002744 1105 100-101 0.8444113176131047 1106 1 0.20790456628556342 1106 2 0.23515839218312706 1106 3 0.12690998724703917 1106 4 0.06875666784480217 1106 5 0.11230965097281853 1106 6 0.1958850024389207 1106 7 0.21411829943158978 1106 8 0.0914906512207665 1106 9 0.3290054716099462 1106 10-11 0.33119854884285616 1106 12-13 0.22901968120569194 1106 14-15 0.3364168501235767 1106 16-17 0.31609414545803816 1106 18-19 0.3212249860073584 1106 20-21 0.3163352894991007 1106 22-23 0.26295110551018297 1106 24-25 0.2794350714732019 1106 26-27 0.2832780237415591 1106 28-29 0.2980005228802014 1106 30-31 0.4258601397079289 1106 32-33 0.40443845649207333 1106 34-35 0.5004860691746984 1106 36-37 0.48695779336365064 1106 38-39 0.5111755836742304 1106 40-41 0.40182746022313864 1106 42-43 0.41378867442626444 1106 44-45 0.4591155024393814 1106 46-47 0.39271638418475874 1106 48-49 0.26588622785303784 1106 50-51 0.21650744896459884 1106 52-53 0.2842157324114254 1106 54-55 0.4030151286326671 1106 56-57 0.40325404311067103 1106 58-59 0.40238490965928264 1106 60-61 0.43907319533384026 1106 62-63 0.44211201178089965 1106 64-65 0.461700135441383 1106 66-67 0.306324857057227 1106 68-69 0.36863882709555185 1106 70-71 0.4596031263791289 1106 72-73 0.370998177498727 1106 74-75 0.5439625556719889 1106 76-77 0.42072935901539665 1106 78-79 0.5083299781279287 1106 80-81 0.2969536576852363 1106 82-83 0.4081823764058008 1106 84-85 0.361582742444039 1106 86-87 0.30770049048416936 1106 88-89 0.2895127367111172 1106 90-91 0.31234009912460436 1106 92-93 0.34097595378790757 1106 94-95 0.3915134632667616 1106 96-97 0.42230704894224047 1106 98-99 0.5771708797374515 1106 100-101 0.5776577039988453 1107 1 0.14178138259213213 1107 2 0.17284712047310435 1107 3 0.1499955029476716 1107 4 0.003119541947654625 1107 5 0.09152882216252323 1107 6 0.12798257965633297 1107 7 0.08797069393512658 1107 8 0.08143468025343026 1107 9 0.20350780119429146 1107 10-11 0.21181190324364962 1107 12-13 0.2439813799999868 1107 14-15 0.2587522091931973 1107 16-17 0.25640621091382343 1107 18-19 0.2922833657106523 1107 20-21 0.2665422356663356 1107 22-23 0.2632394700743461 1107 24-25 0.13351777116008634 1107 26-27 0.2658410719036439 1107 28-29 0.2378826427164924 1107 30-31 0.39800713280130395 1107 32-33 0.5207651304372263 1107 34-35 0.37595419708668487 1107 36-37 0.26882244930682475 1107 38-39 0.2244475627395559 1107 40-41 0.2311827127316448 1107 42-43 0.4045868348387174 1107 44-45 0.5486554866387863 1107 46-47 0.34690506528104237 1107 48-49 0.4415998372483685 1107 50-51 0.48833712955624975 1107 52-53 0.4965334328675297 1107 54-55 0.5468770882185297 1107 56-57 0.5386257844238784 1107 58-59 0.5030014747170881 1107 60-61 0.5919251803299588 1107 62-63 0.37229471451494334 1107 64-65 0.40636795507082724 1107 66-67 0.3898186613067409 1107 68-69 0.3906238380838687 1107 70-71 0.38246003140982765 1107 72-73 0.2851902104614794 1107 74-75 0.438695368809519 1107 76-77 0.5276923599094765 1107 78-79 0.6105522432910391 1107 80-81 0.5704050173464523 1107 82-83 0.569257768131969 1107 84-85 0.6048455651912477 1107 86-87 0.6156543358033382 1107 88-89 0.6359063959608235 1107 90-91 0.6688042218906141 1107 92-93 0.41936354994968283 1107 94-95 0.5798746086686499 1107 96-97 0.6309995683343885 1107 98-99 0.9004513388019468 1107 100-101 1.0930599391215026 1108 1 0.12073019707526456 1108 2 0.10637018105958873 1108 3 0.16695882729587908 1108 4 0.15007425245580208 1108 5 0.2368547000687613 1108 6 0.1942779601133111 1108 7 0.15021497055820277 1108 8 0.14605258961504575 1108 9 0.14191847344510222 1108 10-11 0.17813178859046275 1108 12-13 0.23405430061156807 1108 14-15 0.21802378277840972 1108 16-17 0.1840880112063985 1108 18-19 0.18384662916792394 1108 20-21 0.1605808642835953 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66-67 -0.8716257578477311 2316 68-69 -0.5840155139689642 2316 70-71 -0.8056334907945626 2316 72-73 -1.4558857025321217 2316 74-75 -2.488060916274687 2316 76-77 -1.5455851450549574 2316 78-79 -1.2353479695592355 2316 80-81 -0.928249327756447 2316 82-83 -0.49127575240724397 2316 84-85 -0.5148271633200423 2316 86-87 -0.5310167767326632 2316 88-89 -0.5052465313116414 2316 90-91 -0.5138235572057255 2316 92-93 -0.34012983179622935 2316 94-95 -1.019795086847875 2316 96-97 -0.6641317722330804 2316 98-99 -0.9210647585069047 2316 100-101 -1.3026351816495385 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 9.0 4 26.0 5 37.0 6 103.0 7 279.0 8 790.0 9 1365.0 10 2501.0 11 4059.0 12 6127.0 13 8004.0 14 9673.0 15 12240.0 16 14647.0 17 17095.0 18 14697.0 19 11218.0 20 10914.0 21 11272.0 22 12540.0 23 14575.0 24 18190.0 25 23054.0 26 29427.0 27 38165.0 28 49212.0 29 60484.0 30 74434.0 31 89903.0 32 107625.0 33 131814.0 34 164115.0 35 202834.0 36 264310.0 37 358147.0 38 335372.0 39 62859.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.00662333745905 22.055158710336293 14.951213014303077 22.987004937901588 2 14.950243406066072 21.968432372428627 40.867520033302114 22.21380418820319 3 18.324512405340563 26.893755263996503 31.08413930390598 23.697593026756945 4 12.320138468708794 18.461166402435804 39.475210635701465 29.743484493153932 5 12.003256123732701 38.847174942647825 36.35587027307038 12.793698660549103 6 28.85133125729316 40.14307194436595 17.86933157087297 13.136265227467927 7 25.110735453614137 33.60423840324494 23.69472766501629 17.59029847812463 8 23.789094134879594 37.997873384514556 20.687417586712307 17.525614893893543 9 24.619924287798867 16.731563940271997 21.793227527420814 36.855284244508326 10-11 23.432648747649893 27.851729887356708 29.347959308738815 19.36766205625458 12-13 24.048637111003707 25.254875389372078 29.749044347369612 20.9474431522546 14-15 21.18674365846721 27.038505921230637 27.35613178640557 24.41861863389659 16-17 20.378399128639053 30.082904166483594 28.328527127122033 21.21016957775532 18-19 20.2144639442033 28.832866587886507 30.65657324820143 20.296096219708758 20-21 20.85822578146138 28.052272406672106 30.819028414044453 20.270473397822062 22-23 20.658480599446193 27.823571119022038 30.686860034715785 20.831088246815984 24-25 20.500121061931676 28.098231524527005 30.83221926293056 20.569428150610754 26-27 20.45757495587566 28.306642887008387 30.687114027654953 20.548668129461 28-29 20.402010979913328 28.33058141739858 30.76815455129895 20.49925305138914 30-31 20.682344735270703 28.07133299198289 30.720661091276092 20.525661181470316 32-33 20.646032168611466 28.325346191094198 30.47365191387256 20.554969726421778 34-35 20.701167417407 28.232716551349625 30.680324312422698 20.385791718820688 36-37 20.74922575703756 28.077107013882724 30.744406395228367 20.429260833851345 38-39 20.522698008628538 28.31224608348812 30.770657426387338 20.394398481496008 40-41 20.661228886513108 28.2202613159317 30.673460076115973 20.445049721439222 42-43 20.6279804532914 28.301629331811828 30.328574630122553 20.741815584774216 44-45 20.577694808258386 28.60231307343481 30.24740838456742 20.572583733739382 46-47 20.752779936352873 28.483547032267616 29.997363454706928 20.766309576672587 48-49 20.798847133479544 28.648968935187334 29.766982104527212 20.785201826805906 50-51 20.852249612407412 28.862465561483354 29.44156086060845 20.843723965500786 52-53 20.828257375707913 28.961550518106527 29.33458999458719 20.875602111598372 54-55 20.806240278476473 28.91305127182103 29.415534777756573 20.865173671945925 56-57 20.74052026658563 28.99012352873973 29.40672717525891 20.862629029415725 58-59 20.71046277107542 28.907579934867883 29.64732440233884 20.73463289171786 60-61 20.784404160409427 29.060305191157767 29.476466532283645 20.678824116149165 62-63 20.708090512564404 28.96691348020064 29.57063809832656 20.754357908908396 64-65 20.571263122683455 28.832709097690696 29.583576834301194 21.012450945324655 66-67 20.59345102013232 28.964835151077605 29.409296288027324 21.03241754076275 68-69 20.615146438819885 28.984602445044004 29.371314803472416 21.0289363126637 70-71 20.69315688435208 28.68811115803684 29.552476898092927 21.066255059518152 72-73 20.74796370595094 28.849386737672333 29.39235215993322 21.01029739644351 74-75 20.70982473117108 29.06268814778769 29.214353956282135 21.013133164759097 76-77 20.57158308662374 29.088723465830114 29.271980208549834 21.067713238996312 78-79 20.484176834626876 29.081414603010145 29.349543482483973 21.084865079879002 80-81 20.621890081306226 29.119534423043525 29.15470799804545 21.103867497604796 82-83 20.69769001473081 29.01803078901179 29.350711218309677 20.933567977947725 84-85 20.793197464543148 29.010700071919654 29.097859291857354 21.098243171679844 86-87 20.54427683747777 29.080469073392734 29.190036699836043 21.185217389293452 88-89 20.62167790364374 28.996765924382522 29.110149354861463 21.271406817112272 90-91 20.58587569955109 28.942653635283623 29.352156544958248 21.11931412020704 92-93 20.659922557700668 28.99980733099771 29.124568027076354 21.215702084225267 94-95 20.342526403375636 28.899535008277905 29.25273630989396 21.5052022784525 96-97 20.4767509891843 28.768601405555156 29.287486674051344 21.467160931209197 98-99 20.36077765726932 29.000617445256655 29.09480675350984 21.54379814396418 100-101 20.36838587784759 28.866999516681428 29.06173758807845 21.70287701739253 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 118.0 1 96.5 2 123.5 3 195.0 4 173.5 5 175.0 6 331.5 7 722.0 8 1243.0 9 1583.0 10 1516.5 11 1339.0 12 1414.0 13 1626.5 14 1888.5 15 2190.5 16 2494.5 17 2805.0 18 3215.0 19 3622.0 20 4116.5 21 4803.5 22 5709.0 23 7165.5 24 9280.5 25 12024.5 26 15728.0 27 19773.5 28 24087.5 29 29716.0 30 35555.0 31 41650.5 32 49613.0 33 56532.5 34 61969.5 35 67626.5 36 75581.0 37 83433.0 38 86068.5 39 86761.5 40 87049.0 41 85876.0 42 84594.5 43 85434.0 44 86668.5 45 88764.5 46 89633.0 47 87623.0 48 85375.0 49 82071.0 50 77655.5 51 71429.0 52 63714.5 53 56694.5 54 50323.5 55 42514.0 56 33969.5 57 26694.0 58 21104.5 59 16499.0 60 12142.5 61 8391.0 62 5695.5 63 3581.5 64 2055.0 65 1137.5 66 621.0 67 328.0 68 173.5 69 106.5 70 66.0 71 38.0 72 28.0 73 25.5 74 18.5 75 11.0 76 6.0 77 4.5 78 3.5 79 2.5 80 2.0 81 0.5 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0038387982249027007 2 0.005087563912521651 3 0.0019425244029628124 4 0.16969338177310853 5 0.0025437819562608255 6 2.7750348613754457E-4 7 0.0 8 6.475081343209374E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.7750348613754457E-4 24-25 8.556357489240959E-4 26-27 0.0029137866044442184 28-29 2.312529051146205E-4 30-31 0.00619757785707183 32-33 0.019957125711391747 34-35 0.012441406295166582 36-37 0.0010637633635272544 38-39 3.237540671604687E-4 40-41 0.001826897950405502 42-43 0.01093826241192155 44-45 0.007538844706736629 46-47 0.009943874919928682 48-49 0.010152002534531841 50-51 0.18227353981134387 52-53 0.1130364200200265 54-55 0.017228341431039226 56-57 0.005920074370934285 58-59 0.017343967883596538 60-61 0.12191653157642793 62-63 0.23627109315560776 64-65 0.2419136640404045 66-67 0.25338380813408967 68-69 0.2480881166069649 70-71 0.23412044113804178 72-73 0.15882449523272135 74-75 0.25065502385373717 76-77 0.1958018347605492 78-79 0.005318816817636272 80-81 2.312529051146205E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.1562645255731025E-4 90-91 0.005711946756331126 92-93 0.018338355375589404 94-95 0.0024744060847264396 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2162135.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.54316603198704 #Duplication Level Percentage of deduplicated Percentage of total 1 72.73665519013672 38.94550805456882 2 15.439271256008247 16.533349285466695 3 5.143798824132236 8.26245823426956 4 2.2714259282586875 4.864773424244608 5 1.2122829829422908 3.2454734516715793 6 0.7216501946376065 2.3183661713098282 7 0.470429436100813 1.7631796982435912 8 0.3384186945547117 1.4496006680696991 9 0.2535569227327788 1.2218616371196795 >10 1.2672180564795865 12.259023807238755 >50 0.10044059322650714 3.7131851708883756 >100 0.0431181596174608 4.0724356092950655 >500 0.0010402567033537182 0.35526447544477263 >1k 5.2012835167681E-4 0.45190161130520534 >5k 1.7337611722560334E-4 0.5436187008634517 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6074 0.280926029133241 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5638 0.2607607758072461 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3213 0.14860311682665514 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 1.850023240916964E-4 0.0 26-27 0.0 0.0 0.0 3.468793576719307E-4 0.0 28-29 0.0 0.0 0.0 7.400092963667856E-4 0.0 30-31 0.0 0.0 0.0 0.0026362831183066736 0.0 32-33 0.0 0.0 0.0 0.008024475807477331 0.0 34-35 0.0 0.0 0.0 0.01641895626313806 0.0 36-37 0.0 0.0 0.0 0.02939224424006827 0.0 38-39 0.0 0.0 0.0 0.04909499175583393 0.0 40-41 0.0 0.0 0.0 0.07353842382644932 0.0 42-43 0.0 0.0 0.0 0.10008625733360776 0.0 44-45 0.0 0.0 0.0 0.13116664778101275 0.0 46-47 0.0 0.0 0.0 0.1684908666665125 0.0 48-49 0.0 0.0 0.0 0.21067139655941927 0.0 50-51 0.0 0.0 0.0 0.25615884299546515 0.0 52-53 0.0 0.0 0.0 0.30400506906368013 0.0 54-55 0.0 0.0 0.0 0.358002622407944 0.0 56-57 0.0 0.0 0.0 0.42189780009111366 0.0 58-59 0.0 0.0 0.0 0.4945343375876159 0.0 60-61 0.0 0.0 0.0 0.5734378288127244 0.0 62-63 0.0 0.0 0.0 0.6533588328203374 0.0 64-65 0.0 0.0 0.0 0.7406105539200836 0.0 66-67 0.0 0.0 0.0 0.8426393356566542 0.0 68-69 0.0 0.0 0.0 0.9577570318227122 0.0 70-71 0.0 0.0 0.0 1.0897561900621375 0.0 72-73 0.0 0.0 0.0 1.2289935642316507 2.312529051146205E-5 74-75 0.0 0.0 0.0 1.372994748246525 4.62505810229241E-5 76-77 0.0 0.0 0.0 1.53947371463854 4.62505810229241E-5 78-79 0.0 0.0 0.0 1.724591665182794 4.62505810229241E-5 80-81 0.0 0.0 0.0 1.9339911707640827 4.62505810229241E-5 82-83 0.0 0.0 0.0 2.1525251660973987 4.62505810229241E-5 84-85 0.0 0.0 0.0 2.378921760204612 4.62505810229241E-5 86-87 0.0 0.0 0.0 2.625021101827592 6.937587153438616E-5 88-89 0.0 0.0 0.0 2.9025939638366705 9.25011620458482E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2930 0.0 64.38638 1 GTATCAA 5760 0.0 42.15699 1 ACGCGAG 70 2.0677512E-4 33.889854 7 ATCAACG 7175 0.0 33.37563 3 TCAACGC 7315 0.0 32.801815 4 CAACGCA 7445 0.0 31.929405 5 TATCAAC 7685 0.0 31.223997 2 AACGCAG 7755 0.0 30.896358 6 CGCGAGT 85 6.4519525E-4 27.90929 8 CGGGCGT 260 0.0 27.372574 6 ACGCAGA 8795 0.0 27.02711 7 CGCAGAG 8865 0.0 26.867218 8 GGCGTCG 245 1.0913936E-11 25.17532 8 TATCACG 155 1.4074303E-6 24.52445 2 ACGGGCG 265 3.274181E-11 23.276373 5 GCAGAGT 10055 0.0 23.074087 9 CGCGGAA 95 3.3232573E-7 22.477442 32-33 GTACATG 6120 0.0 22.284435 1 GAGTACT 7345 0.0 21.2198 12-13 CAGAGTA 9740 0.0 21.019363 10-11 >>END_MODULE