FastQCFastQC Report
Sat 18 Jun 2016
SRR1931051_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931051_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966068
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN860814.3783327941861625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15950.058.755451
GTATCAA30850.049.633371
ATCAACG39050.038.3016933
TCAACGC39400.038.087824
CAACGCA40150.037.3692555
TATCAAC41450.036.438022
AACGCAG43950.034.393826
ACGCAGA47800.031.0165277
CGCAGAG48800.029.8585998
GCAGAGT52500.025.2819089
GTACATG31500.025.1987441
TACATGG31750.023.543252
ACATGGG33500.021.9477793
AGAGTAC46850.021.13221210-11
GAGTACT35450.021.13158812-13
CAGAGTA50600.020.92484510-11
GTATAAC5150.019.8875141
AGTACTT38650.019.31989112-13
CATGGGT9150.019.2625164
GTGGTAT8850.019.0953851
Produced by FastQC (version 0.11.5)