##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931051_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1966068 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.080707279707518 27.0 2.0 31.0 2.0 31.0 2 18.95639265783279 26.0 2.0 31.0 2.0 33.0 3 19.48026060136272 26.0 2.0 31.0 2.0 33.0 4 21.041463469218765 28.0 2.0 35.0 2.0 37.0 5 20.46294227870043 28.0 2.0 35.0 2.0 37.0 6 21.26114915659072 28.0 2.0 35.0 2.0 37.0 7 21.346912212598955 28.0 2.0 35.0 2.0 37.0 8 21.66242876645162 28.0 2.0 35.0 2.0 37.0 9 22.738465810948554 30.0 2.0 37.0 2.0 39.0 10-11 22.9602371332019 30.5 2.0 37.0 2.0 39.0 12-13 22.7879353613405 30.0 2.0 37.0 2.0 39.0 14-15 22.694169275935522 30.5 2.0 37.0 2.0 39.5 16-17 21.685116181129032 27.0 2.0 37.0 2.0 39.0 18-19 20.79480643599306 26.5 2.0 36.0 2.0 39.0 20-21 20.475773727053184 25.5 2.0 36.0 2.0 39.0 22-23 20.12563044614937 25.0 2.0 36.0 2.0 39.0 24-25 19.924020684940704 24.0 2.0 36.0 2.0 39.0 26-27 19.62284493720461 23.5 2.0 36.0 2.0 39.0 28-29 19.379614031661163 21.0 2.0 35.5 2.0 39.0 30-31 19.252267724208927 22.0 2.0 35.0 2.0 39.0 32-33 19.06911892162428 21.0 2.0 35.0 2.0 39.0 34-35 18.696117835191863 18.0 2.0 35.0 2.0 38.0 36-37 18.271270118836174 16.0 2.0 34.0 2.0 38.0 38-39 18.059039412675453 16.0 2.0 34.0 2.0 38.0 40-41 17.853082141614635 15.0 2.0 34.0 2.0 38.0 42-43 17.650483096210305 15.0 2.0 34.0 2.0 38.0 44-45 17.25229849628802 11.0 2.0 33.0 2.0 38.0 46-47 16.900694177414007 9.0 2.0 33.0 2.0 38.0 48-49 16.580599195958634 9.0 2.0 33.0 2.0 38.0 50-51 14.665858708854424 5.5 2.0 30.0 2.0 37.0 52-53 16.364924305771723 8.5 2.0 32.5 2.0 37.5 54-55 16.967900143840396 8.0 2.0 34.0 2.0 38.0 56-57 16.886364306829673 7.5 2.0 34.0 2.0 38.0 58-59 16.602452712724077 4.0 2.0 34.0 2.0 38.0 60-61 16.291290789535253 2.0 2.0 33.5 2.0 38.0 62-63 15.846229631935415 2.0 2.0 33.0 2.0 38.0 64-65 15.465626061763885 2.0 2.0 32.0 2.0 38.0 66-67 15.068030709009047 2.0 2.0 31.5 2.0 37.0 68-69 14.614457383976546 2.0 2.0 31.0 2.0 36.5 70-71 14.231250139873087 2.0 2.0 31.0 2.0 36.0 72-73 13.817161207038618 2.0 2.0 30.0 2.0 35.5 74-75 13.361806153195108 2.0 2.0 29.5 2.0 35.0 76-77 12.917000836186745 2.0 2.0 29.0 2.0 34.5 78-79 12.511113806846964 2.0 2.0 29.0 2.0 34.0 80-81 12.057596176734478 2.0 2.0 28.0 2.0 34.0 82-83 11.59412543208068 2.0 2.0 26.5 2.0 34.0 84-85 11.177829301936658 2.0 2.0 26.0 2.0 33.5 86-87 10.694191401314704 2.0 2.0 25.5 2.0 33.0 88-89 10.271265795486219 2.0 2.0 24.5 2.0 32.5 90-91 9.787731909577898 2.0 2.0 21.0 2.0 32.0 92-93 9.271722799007968 2.0 2.0 18.5 2.0 32.0 94-95 8.736642883155618 2.0 2.0 9.0 2.0 31.5 96-97 8.150640262696916 2.0 2.0 2.0 2.0 31.0 98-99 7.558592073112425 2.0 2.0 2.0 2.0 31.0 100-101 6.814887887906217 2.0 2.0 2.0 2.0 30.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 8.059894918572219 1101 2 9.508370919666586 1101 3 8.984592079218679 1101 4 10.909784077087917 1101 5 11.859313287490359 1101 6 10.892375454603627 1101 7 10.709124113086819 1101 8 10.244026323548777 1101 9 10.447030345039519 1101 10-11 10.029834359018249 1101 12-13 9.822009891312224 1101 14-15 10.438344556496066 1101 16-17 11.314595125740109 1101 18-19 11.74726711112239 1101 20-21 11.972798986535114 1101 22-23 12.132751646219258 1101 24-25 11.92215822249264 1101 26-27 12.144817384879769 1101 28-29 11.778680911085466 1101 30-31 11.492958235173901 1101 32-33 11.445645225036532 1101 34-35 11.489316539657153 1101 36-37 11.803818774092967 1101 38-39 11.635072541169183 1101 40-41 11.161970228299388 1101 42-43 11.101406732799905 1101 44-45 10.813381608543807 1101 46-47 10.571871261113948 1101 48-49 10.798050577971548 1101 50-51 12.29270071055992 1101 52-53 11.477538820361444 1101 54-55 11.653505721127797 1101 56-57 11.558137899183851 1101 58-59 11.18046779359008 1101 60-61 10.95891476173985 1101 62-63 11.039122765644958 1101 64-65 10.654494986328933 1101 66-67 10.220640467496183 1101 68-69 10.193324116088336 1101 70-71 10.119051305126584 1101 72-73 9.916646810868722 1101 74-75 9.559595704536177 1101 76-77 9.496580154237826 1101 78-79 9.037367781573163 1101 80-81 8.742854399822479 1101 82-83 8.478249678440166 1101 84-85 8.319989070802949 1101 86-87 7.9116234470884255 1101 88-89 7.6106852218282945 1101 90-91 7.391122135829656 1101 92-93 7.0090275066692325 1101 94-95 6.890306071423334 1101 96-97 6.739261645002146 1101 98-99 5.887544236829764 1101 100-101 5.0205274601801575 1102 1 7.825722288095697 1102 2 8.84371449329782 1102 3 8.425458722611943 1102 4 10.187978351830456 1102 5 10.965714928265363 1102 6 10.151251349175105 1102 7 10.076659168652075 1102 8 9.6293800792944 1102 9 9.52302091931077 1102 10-11 8.868880585817571 1102 12-13 9.23289311700606 1102 14-15 9.599128870221552 1102 16-17 10.304844296625085 1102 18-19 11.114359972005616 1102 20-21 11.127971500644616 1102 22-23 11.201638013822677 1102 24-25 10.930719926105837 1102 26-27 11.2553536913905 1102 28-29 10.87745396630162 1102 30-31 10.769370639898405 1102 32-33 10.710361289503862 1102 34-35 10.533917837149446 1102 36-37 10.661741913675211 1102 38-39 10.685755470127116 1102 40-41 10.416874370965353 1102 42-43 10.017943941155057 1102 44-45 9.96213575411895 1102 46-47 9.7278270707375 1102 48-49 9.460473484819747 1102 50-51 10.952127166602278 1102 52-53 10.439566806630138 1102 54-55 10.197701188632305 1102 56-57 10.049278005077841 1102 58-59 10.1943983806617 1102 60-61 9.943248851458824 1102 62-63 10.04854413063012 1102 64-65 9.65955622447404 1102 66-67 9.517703654788901 1102 68-69 9.266222236760933 1102 70-71 9.020102244790285 1102 72-73 9.027043476953553 1102 74-75 8.484313514508829 1102 76-77 8.211571135793655 1102 78-79 7.983317481928083 1102 80-81 7.463322631625589 1102 82-83 7.257249067513305 1102 84-85 7.128247492866139 1102 86-87 6.666251945372013 1102 88-89 6.750775842646352 1102 90-91 6.335849255164037 1102 92-93 5.970111465475307 1102 94-95 5.778318813612197 1102 96-97 5.507450537769935 1102 98-99 5.024202757804919 1102 100-101 4.2490279256482415 1103 1 7.947852326551757 1103 2 8.977548704948582 1103 3 8.407589710600359 1103 4 10.365345061226076 1103 5 11.150692139488836 1103 6 10.285396532672234 1103 7 10.055644830713508 1103 8 9.825711323656549 1103 9 9.386010521931478 1103 10-11 9.26081727574778 1103 12-13 8.76710476776806 1103 14-15 9.222525347456514 1103 16-17 10.315836914011022 1103 18-19 10.622714669816943 1103 20-21 10.941234093817478 1103 22-23 11.36840438138665 1103 24-25 11.11242006378803 1103 26-27 10.98832679588654 1103 28-29 10.588914228372552 1103 30-31 10.561370829804876 1103 32-33 10.530468824043101 1103 34-35 10.371020220475657 1103 36-37 10.221454442754641 1103 38-39 10.160009493770474 1103 40-41 9.915445918228329 1103 42-43 9.471736135999098 1103 44-45 9.460320366947133 1103 46-47 9.112292127946827 1103 48-49 9.410795732362832 1103 50-51 11.158597636146027 1103 52-53 10.387455284055006 1103 54-55 10.174485716002575 1103 56-57 9.633986973410938 1103 58-59 9.60108902302034 1103 60-61 9.356285930697299 1103 62-63 9.362769312228185 1103 64-65 9.03659000408758 1103 66-67 8.980255285610722 1103 68-69 8.614971227882807 1103 70-71 8.288130896929106 1103 72-73 8.208454600725092 1103 74-75 7.825569508431821 1103 76-77 7.5100993606429185 1103 78-79 6.8643036899706225 1103 80-81 6.614484028387684 1103 82-83 6.445436291555465 1103 84-85 6.525354386471038 1103 86-87 6.298562157592494 1103 88-89 5.928851734019853 1103 90-91 5.390287132009691 1103 92-93 5.1698906992026945 1103 94-95 4.696600872198344 1103 96-97 4.328944983005656 1103 98-99 3.754148634460667 1103 100-101 3.183375478228597 1104 1 8.131654814878633 1104 2 9.22824257651573 1104 3 8.620790199748686 1104 4 10.559564175011243 1104 5 11.105015179841246 1104 6 10.616554606470338 1104 7 10.238904648295154 1104 8 9.819315395083375 1104 9 9.617841000154257 1104 10-11 9.175066455167713 1104 12-13 9.141898968265355 1104 14-15 9.54053901428879 1104 16-17 10.438437568148014 1104 18-19 10.907427737314528 1104 20-21 11.243730798116609 1104 22-23 11.482921521940003 1104 24-25 11.161025397645727 1104 26-27 11.255078206376698 1104 28-29 11.056440666700183 1104 30-31 11.092536350199822 1104 32-33 10.991676126827546 1104 34-35 10.944004781586038 1104 36-37 10.875102053502406 1104 38-39 10.310901398272524 1104 40-41 9.917423338750453 1104 42-43 9.620679475541806 1104 44-45 9.938883476273649 1104 46-47 9.861017538178682 1104 48-49 9.373073832503273 1104 50-51 10.723531727946277 1104 52-53 10.496711985701284 1104 54-55 9.986475085985575 1104 56-57 9.665114329997046 1104 58-59 9.66354712136894 1104 60-61 9.276469116979738 1104 62-63 9.417345187814167 1104 64-65 9.182037050057518 1104 66-67 8.679288158845212 1104 68-69 8.651437678055183 1104 70-71 8.315270833609764 1104 72-73 8.065182310093727 1104 74-75 7.7512513083673635 1104 76-77 7.268186073936139 1104 78-79 6.978688886136091 1104 80-81 6.68672637322795 1104 82-83 6.60967331207898 1104 84-85 6.2456920896380534 1104 86-87 5.905205220098525 1104 88-89 5.5650823365077144 1104 90-91 5.178453442442025 1104 92-93 4.977046762419649 1104 94-95 4.659373123851431 1104 96-97 3.8881007483587355 1104 98-99 3.285382337757997 1104 100-101 3.1797542214524848 1105 1 7.900064979717801 1105 2 9.123666587771972 1105 3 8.428644997121747 1105 4 10.28647991154525 1105 5 10.19468106017267 1105 6 10.225961687856035 1105 7 9.799965764017493 1105 8 9.442231015123276 1105 9 9.458916641235906 1105 10-11 8.917747007386197 1105 12-13 8.769930772977542 1105 14-15 9.473145692859699 1105 16-17 10.110069341188368 1105 18-19 10.704344374857666 1105 20-21 10.879171923995774 1105 22-23 10.901284292788812 1105 24-25 10.998781980473037 1105 26-27 11.038979471247899 1105 28-29 10.598986386087716 1105 30-31 10.33997837162746 1105 32-33 10.487124324263217 1105 34-35 10.4492754574703 1105 36-37 10.416421058060237 1105 38-39 10.058115095795568 1105 40-41 9.820598190038439 1105 42-43 9.478622417090886 1105 44-45 9.481133637363158 1105 46-47 9.432074508348908 1105 48-49 9.260746434908878 1105 50-51 10.709438329703882 1105 52-53 9.961764890409105 1105 54-55 9.741949740325708 1105 56-57 9.655736895452172 1105 58-59 9.755085212212503 1105 60-61 9.662138111708378 1105 62-63 9.349538448811941 1105 64-65 8.854127859946411 1105 66-67 8.837375557640952 1105 68-69 8.724129433730633 1105 70-71 8.652062472063013 1105 72-73 8.37263449745266 1105 74-75 8.044501794366225 1105 76-77 7.659178644706312 1105 78-79 7.408431675332714 1105 80-81 6.967180140538245 1105 82-83 6.695244834001718 1105 84-85 6.233453040994478 1105 86-87 6.172376611147412 1105 88-89 5.647817954945818 1105 90-91 5.293923564433202 1105 92-93 5.054745489539478 1105 94-95 4.696049428585459 1105 96-97 4.475543229392039 1105 98-99 3.7340958059332623 1105 100-101 3.31418501120974 1106 1 7.64936492890261 1106 2 8.833951788026763 1106 3 8.430819851475391 1106 4 10.09364096170199 1106 5 10.572314861578317 1106 6 9.873117289693969 1106 7 9.136623555918554 1106 8 8.99906641863883 1106 9 8.767530352748764 1106 10-11 8.292487451804401 1106 12-13 8.160268148265612 1106 14-15 8.711214970604075 1106 16-17 9.578380076076211 1106 18-19 10.648085786374821 1106 20-21 10.690755036354602 1106 22-23 10.616105397375062 1106 24-25 10.332887718116357 1106 26-27 10.527878745342175 1106 28-29 10.63343391850924 1106 30-31 9.994368092823834 1106 32-33 10.314484077022268 1106 34-35 10.135362607310697 1106 36-37 10.515517835947758 1106 38-39 9.754905418722522 1106 40-41 9.933159092222777 1106 42-43 9.760158923510776 1106 44-45 9.478278302304972 1106 46-47 9.153360682859724 1106 48-49 9.373538935374086 1106 50-51 10.977641328336883 1106 52-53 10.10152254310517 1106 54-55 9.784930735623515 1106 56-57 9.69416510020876 1106 58-59 9.342548509867846 1106 60-61 9.10672472834713 1106 62-63 9.136912104177593 1106 64-65 8.992220487628524 1106 66-67 8.48255055245208 1106 68-69 8.182818048444135 1106 70-71 7.9762174235022965 1106 72-73 7.68330754375174 1106 74-75 7.326805896440346 1106 76-77 7.357645399226001 1106 78-79 6.854025313950828 1106 80-81 6.737992785468892 1106 82-83 6.621287037448559 1106 84-85 6.3545107260762865 1106 86-87 6.292364090749766 1106 88-89 5.97283455039965 1106 90-91 5.673324826403398 1106 92-93 5.103345231348213 1106 94-95 4.908029512251597 1106 96-97 4.221049484670378 1106 98-99 3.8055734544066597 1106 100-101 3.2955075212654217 1107 1 6.967888617651543 1107 2 7.808396824072805 1107 3 7.847735513686356 1107 4 9.252411082058675 1107 5 9.965661590868226 1107 6 9.129900689269128 1107 7 9.305735900616643 1107 8 7.868517029411233 1107 9 5.411784045852013 1107 10-11 7.257933604575708 1107 12-13 7.418097184502113 1107 14-15 7.955380197884036 1107 16-17 8.678373823838701 1107 18-19 10.027604502036187 1107 20-21 10.05339104198918 1107 22-23 9.559790092364882 1107 24-25 9.62203401179664 1107 26-27 9.769454967793667 1107 28-29 9.973614712748102 1107 30-31 9.637931592305744 1107 32-33 9.784083962746497 1107 34-35 9.738574699894542 1107 36-37 9.905744342720311 1107 38-39 9.721342250764952 1107 40-41 9.389293972343353 1107 42-43 8.875621062875158 1107 44-45 8.292062305521423 1107 46-47 8.592469340740177 1107 48-49 8.793539553922852 1107 50-51 10.028104672536351 1107 52-53 9.09497604993179 1107 54-55 9.141233625158492 1107 56-57 8.946634455901222 1107 58-59 8.808712891204493 1107 60-61 8.594930249406604 1107 62-63 8.690760334115755 1107 64-65 8.52570233199854 1107 66-67 8.478279005984492 1107 68-69 8.168828611388035 1107 70-71 7.905439424881349 1107 72-73 7.7023700819243235 1107 74-75 7.610777406707255 1107 76-77 7.237152767410031 1107 78-79 7.078713263886648 1107 80-81 6.712296868046488 1107 82-83 6.621221733829005 1107 84-85 6.5555981481541625 1107 86-87 5.875593609498802 1107 88-89 5.743169952904681 1107 90-91 5.435479587749262 1107 92-93 4.780675040881919 1107 94-95 4.361511530171267 1107 96-97 4.082477206468503 1107 98-99 3.5009351841716034 1107 100-101 3.008208307645602 1108 1 6.369144838538226 1108 2 7.347148000227442 1108 3 7.378602665239793 1108 4 8.18060691268876 1108 5 9.885509885435457 1108 6 8.577555455723385 1108 7 8.102171419996733 1108 8 5.367056877154326 1108 9 6.51406078954043 1108 10-11 5.910766026496017 1108 12-13 4.5691092870735055 1108 14-15 6.52760435330303 1108 16-17 7.794527767981698 1108 18-19 7.22388713555609 1108 20-21 7.3580819192678 1108 22-23 8.174875726905007 1108 24-25 7.819673795026407 1108 26-27 6.39456831047471 1108 28-29 8.116727636582503 1108 30-31 8.498378295210166 1108 32-33 8.893904503596463 1108 34-35 8.49377547396324 1108 36-37 7.960402234116884 1108 38-39 7.822775945951701 1108 40-41 8.400606477741302 1108 42-43 7.858586105132407 1108 44-45 7.0713139464408705 1108 46-47 7.946750133431372 1108 48-49 8.22226771630044 1108 50-51 9.392035459382377 1108 52-53 8.800031674725416 1108 54-55 8.242231838337762 1108 56-57 8.38763211304904 1108 58-59 8.4851586470324 1108 60-61 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22-23 8.14767321310865 1109 24-25 7.625770549484635 1109 26-27 8.116077689382305 1109 28-29 8.529780480121943 1109 30-31 6.219966127026396 1109 32-33 5.22860732191398 1109 34-35 6.840044590838119 1109 36-37 5.022503216773977 1109 38-39 5.360171488312485 1109 40-41 6.091686450847092 1109 42-43 5.051686584727424 1109 44-45 5.586125029772315 1109 46-47 5.132748904335806 1109 48-49 5.7661191072223374 1109 50-51 6.977635840911262 1109 52-53 7.633779982654458 1109 54-55 7.809472518785988 1109 56-57 6.966257755726321 1109 58-59 6.511085268788218 1109 60-61 6.656536442455392 1109 62-63 7.042036307017035 1109 64-65 6.925154291581395 1109 66-67 6.331258308513837 1109 68-69 5.78433508645273 1109 70-71 5.4700607559330034 1109 72-73 5.001644974736168 1109 74-75 4.1764208695418805 1109 76-77 4.135142385061499 1109 78-79 4.816400836180165 1109 80-81 5.005688333240515 1109 82-83 4.883268159360567 1109 84-85 4.582164529097417 1109 86-87 4.673463336329531 1109 88-89 4.445145560661583 1109 90-91 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2313 46-47 -14.475359203772303 2313 48-49 -14.182908868121427 2313 50-51 -12.333412049083996 2313 52-53 -13.964844958075746 2313 54-55 -14.559896850583382 2313 56-57 -14.473761210608433 2313 58-59 -14.21295456051477 2313 60-61 -13.899888403443137 2313 62-63 -13.476455869369879 2313 64-65 -13.077453579447527 2313 66-67 -12.695531639328744 2313 68-69 -12.267111096572398 2313 70-71 -11.900731088543049 2313 72-73 -11.488397699517035 2313 74-75 -11.027941387451955 2313 76-77 -10.609997491657325 2313 78-79 -10.227221733758192 2313 80-81 -9.770745995825392 2313 82-83 -9.318486226604344 2313 84-85 -8.910723095369155 2313 86-87 -8.424924525216225 2313 88-89 -8.040155529902666 2313 90-91 -7.5675821173849815 2313 92-93 -7.0681238286423405 2313 94-95 -6.551583147172115 2313 96-97 -5.977108285759477 2313 98-99 -5.402267830430374 2313 100-101 -4.693864231214502 2314 1 -17.646336535433715 2314 2 -16.54157962434924 2314 3 -17.05052166954492 2314 4 -18.561531452091113 2314 5 -17.915167424675815 2314 6 -18.76835649396215 2314 7 -18.859125145073417 2314 8 -19.193659136391876 2314 9 -20.27403790421864 2314 10-11 -20.517148825947135 2314 12-13 -20.361224530052763 2314 14-15 -20.228322110170605 2314 16-17 -19.207165507296484 2314 18-19 -18.315541988778698 2314 20-21 -17.996538303276953 2314 22-23 -17.643460025393008 2314 24-25 -17.440848701345143 2314 26-27 -17.16106787723695 2314 28-29 -16.915928386639557 2314 30-31 -16.791413673827087 2314 32-33 -16.608756357554963 2314 34-35 -16.261425300105458 2314 36-37 -15.826003184364005 2314 38-39 -15.620862176931706 2314 40-41 -15.421115825113077 2314 42-43 -15.218820764727294 2314 44-45 -14.827815226273206 2314 46-47 -14.475359203772303 2314 48-49 -14.182908868121427 2314 50-51 -12.333412049083996 2314 52-53 -13.964844958075746 2314 54-55 -14.559896850583382 2314 56-57 -14.473761210608433 2314 58-59 -14.21295456051477 2314 60-61 -13.899888403443137 2314 62-63 -13.476455869369879 2314 64-65 -13.077453579447527 2314 66-67 -12.695531639328744 2314 68-69 -12.267111096572398 2314 70-71 -11.900731088543049 2314 72-73 -11.488397699517035 2314 74-75 -11.027941387451955 2314 76-77 -10.609997491657325 2314 78-79 -10.227221733758192 2314 80-81 -9.770745995825392 2314 82-83 -9.318486226604344 2314 84-85 -8.910723095369155 2314 86-87 -8.424924525216225 2314 88-89 -8.040155529902666 2314 90-91 -7.5675821173849815 2314 92-93 -7.0681238286423405 2314 94-95 -6.551583147172115 2314 96-97 -5.977108285759477 2314 98-99 -5.402267830430374 2314 100-101 -4.693864231214502 2315 1 -17.646336535433715 2315 2 -16.54157962434924 2315 3 -17.05052166954492 2315 4 -18.561531452091113 2315 5 -17.915167424675815 2315 6 -18.76835649396215 2315 7 -18.859125145073417 2315 8 -19.193659136391876 2315 9 -20.27403790421864 2315 10-11 -20.517148825947135 2315 12-13 -20.361224530052763 2315 14-15 -20.228322110170605 2315 16-17 -19.207165507296484 2315 18-19 -18.315541988778698 2315 20-21 -17.996538303276953 2315 22-23 -17.643460025393008 2315 24-25 -17.440848701345143 2315 26-27 -17.16106787723695 2315 28-29 -16.915928386639557 2315 30-31 -16.791413673827087 2315 32-33 -16.608756357554963 2315 34-35 -16.261425300105458 2315 36-37 -15.826003184364005 2315 38-39 -15.620862176931706 2315 40-41 -15.421115825113077 2315 42-43 -15.218820764727294 2315 44-45 -14.827815226273206 2315 46-47 -14.475359203772303 2315 48-49 -14.182908868121427 2315 50-51 -12.333412049083996 2315 52-53 -13.964844958075746 2315 54-55 -14.559896850583382 2315 56-57 -14.473761210608433 2315 58-59 -14.21295456051477 2315 60-61 -13.899888403443137 2315 62-63 -13.476455869369879 2315 64-65 -13.077453579447527 2315 66-67 -12.695531639328744 2315 68-69 -12.267111096572398 2315 70-71 -11.900731088543049 2315 72-73 -11.488397699517035 2315 74-75 -11.027941387451955 2315 76-77 -10.609997491657325 2315 78-79 -10.227221733758192 2315 80-81 -9.770745995825392 2315 82-83 -9.318486226604344 2315 84-85 -8.910723095369155 2315 86-87 -8.424924525216225 2315 88-89 -8.040155529902666 2315 90-91 -7.5675821173849815 2315 92-93 -7.0681238286423405 2315 94-95 -6.551583147172115 2315 96-97 -5.977108285759477 2315 98-99 -5.402267830430374 2315 100-101 -4.693864231214502 2316 1 -17.646336535433715 2316 2 -16.54157962434924 2316 3 -17.05052166954492 2316 4 -18.561531452091113 2316 5 -17.915167424675815 2316 6 -18.76835649396215 2316 7 -18.859125145073417 2316 8 -19.193659136391876 2316 9 -20.27403790421864 2316 10-11 -20.517148825947135 2316 12-13 -20.361224530052763 2316 14-15 -20.228322110170605 2316 16-17 -19.207165507296484 2316 18-19 -18.315541988778698 2316 20-21 -17.996538303276953 2316 22-23 -17.643460025393008 2316 24-25 -17.440848701345143 2316 26-27 -17.16106787723695 2316 28-29 -16.915928386639557 2316 30-31 -16.791413673827087 2316 32-33 -16.608756357554963 2316 34-35 -16.261425300105458 2316 36-37 -15.826003184364005 2316 38-39 -15.620862176931706 2316 40-41 -15.421115825113077 2316 42-43 -15.218820764727294 2316 44-45 -14.827815226273206 2316 46-47 -14.475359203772303 2316 48-49 -14.182908868121427 2316 50-51 -12.333412049083996 2316 52-53 -13.964844958075746 2316 54-55 -14.559896850583382 2316 56-57 -14.473761210608433 2316 58-59 -14.21295456051477 2316 60-61 -13.899888403443137 2316 62-63 -13.476455869369879 2316 64-65 -13.077453579447527 2316 66-67 -12.695531639328744 2316 68-69 -12.267111096572398 2316 70-71 -11.900731088543049 2316 72-73 -11.488397699517035 2316 74-75 -11.027941387451955 2316 76-77 -10.609997491657325 2316 78-79 -10.227221733758192 2316 80-81 -9.770745995825392 2316 82-83 -9.318486226604344 2316 84-85 -8.910723095369155 2316 86-87 -8.424924525216225 2316 88-89 -8.040155529902666 2316 90-91 -7.5675821173849815 2316 92-93 -7.0681238286423405 2316 94-95 -6.551583147172115 2316 96-97 -5.977108285759477 2316 98-99 -5.402267830430374 2316 100-101 -4.693864231214502 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 553615.0 3 26798.0 4 72358.0 5 43861.0 6 29965.0 7 29304.0 8 28324.0 9 29510.0 10 31334.0 11 31904.0 12 32487.0 13 31881.0 14 32166.0 15 32663.0 16 33168.0 17 33279.0 18 33096.0 19 33603.0 20 34835.0 21 36550.0 22 38140.0 23 39778.0 24 41500.0 25 43824.0 26 45322.0 27 47946.0 28 51091.0 29 53696.0 30 57372.0 31 59947.0 32 62312.0 33 63447.0 34 60364.0 35 50490.0 36 30027.0 37 9369.0 38 738.0 39 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.94162738036581 20.655167274050342 14.275197852602009 23.12800749298184 2 14.836180845661524 21.31231023825968 41.804328371107715 22.047180544971077 3 18.324115244188988 27.025662765821103 30.557521862152598 24.092700127837315 4 12.10414223873048 18.017640338269832 40.17794864714244 29.700268775857246 5 12.303612682093393 38.454432462045425 35.92622017801448 13.315734677846697 6 28.622804547593823 39.470032593744996 18.601527793814686 13.305635064846493 7 25.315446911975798 33.6434318067425 23.728225641080268 17.31289564020144 8 24.035686836110763 37.019394524152304 21.291431943162408 17.653486696574525 9 25.35134665378247 17.139339832601355 21.852066929530384 35.65724658408579 10-11 23.405236024138766 27.4998478056879 29.779013858829057 19.31590231134428 12-13 24.296018530055047 25.088709786372966 29.764156112411268 20.851115571160726 14-15 21.661728091932286 26.741269705512828 27.531620501591714 24.065381700963172 16-17 20.94015042617242 29.706177099923327 28.308442040945692 21.04523043295856 18-19 20.701854274223123 28.5139381396027 30.57247655966173 20.21173102651244 20-21 21.460486479727876 27.86626954908203 30.51008663703602 20.16315733415407 22-23 20.984768140395854 27.904384360564656 30.31860666462466 20.792240834414834 24-25 20.841706741575763 28.0515453646351 30.536021942292823 20.570725951496314 26-27 20.761859845575838 28.20572467716768 30.26545702137061 20.766958455885867 28-29 20.83112421627324 28.40153047629923 30.289679820769233 20.477665486658292 30-31 21.118290059314873 28.078598740538794 30.281732586598846 20.521378613547483 32-33 20.88272812606374 28.236056622781835 30.14320736357479 20.73800788757964 34-35 21.1571819160871 28.296043142679682 30.165619596145742 20.381155345087475 36-37 21.220871051765585 28.165977834669164 29.942125562639095 20.67102555092616 38-39 21.127229320939797 28.28588670642321 30.029950115735403 20.556933856901587 40-41 21.370535559935 28.198910464980838 29.964797727075137 20.465756248009026 42-43 21.196336347673515 28.18101386668912 29.669737590800267 20.9529121948371 44-45 21.191494904758564 28.489397021346242 29.651165509340444 20.667942564554757 46-47 21.339691330754757 28.454362970011392 29.19449830525849 21.01144739397536 48-49 21.245566948189662 28.543668226890862 29.257551202856057 20.953213622063423 50-51 21.44933298478916 28.767911674472945 28.9642684445721 20.818486896165787 52-53 21.03327419421874 29.02663338251491 28.980752945699383 20.959339477566967 54-55 20.889503410396703 28.899554065593474 29.161428518717646 21.049514005292178 56-57 20.952589328111166 29.023126512679582 29.16324306309614 20.861041096113112 58-59 20.95069436032921 28.985337050546427 29.213966458515706 20.85000213060866 60-61 20.9217146239984 29.091842945644654 29.20203780444081 20.784404625916135 62-63 20.9589729425741 29.047482770898707 29.18834377672549 20.8052005098017 64-65 20.70950512246244 28.853600088802406 29.283540865431707 21.15335392330345 66-67 20.64141928839056 29.021350517947326 28.956609156400344 21.380621037261776 68-69 20.923453256564713 28.91949279462176 29.040636336406294 21.11641761240723 70-71 21.03486854798665 28.724838262001683 29.025608747644977 21.21468444236669 72-73 20.932719923637727 28.878313434017777 28.945772423351762 21.243194218992738 74-75 20.897437352031282 29.208583257064046 28.616456264292612 21.277523126612056 76-77 20.95038473617836 28.97346534165694 28.74185549114026 21.334294431024443 78-79 20.781031094863753 29.11511213711016 28.691737987415415 21.41211878061067 80-81 20.830847047136093 29.204215269638954 28.326131490180927 21.638806193044026 82-83 21.065931639450383 28.983594767360408 28.497419016368486 21.453054576820723 84-85 21.059772736606337 29.025806431231814 28.291259804959108 21.623161027202737 86-87 20.97166448222227 29.044147245506863 28.448196464742427 21.535991807528443 88-89 21.046845210758853 28.97265789021833 28.35779446429314 21.622702434729675 90-91 21.035016724453758 29.01349867846232 28.340110036925793 21.611374560158133 92-93 21.02613003645969 29.018104977782727 28.299080832126755 21.656684153630827 94-95 20.990185252523517 28.88136820462115 28.26358407689168 21.864862465963654 96-97 20.908454388507 28.889700683256773 28.221351217079565 21.980493711156658 98-99 20.76468193235204 28.923569500071494 27.993759732894635 22.317988834681827 100-101 21.078170424487112 29.06047810847785 27.979210352084593 21.882141114950446 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 116171.0 1 105142.0 2 69674.0 3 35538.5 4 22033.5 5 17313.5 6 16135.5 7 16332.5 8 16800.5 9 16635.5 10 15415.0 11 13122.0 12 10539.0 13 8036.5 14 6073.5 15 4919.0 16 4503.5 17 4787.0 18 5576.5 19 6670.5 20 7975.5 21 9218.0 22 10573.0 23 12168.5 24 13891.5 25 15926.5 26 18285.5 27 20936.0 28 24116.5 29 27507.5 30 30928.5 31 34522.5 32 38391.0 33 42020.5 34 44668.0 35 47388.0 36 50840.5 37 53776.0 38 55754.0 39 56778.0 40 57334.5 41 57802.0 42 58068.5 43 58307.5 44 58007.5 45 57802.0 46 57146.0 47 55719.5 48 53457.0 49 50702.0 50 47836.5 51 44146.0 52 40363.0 53 36546.0 54 32317.0 55 27755.0 56 23347.5 57 19370.5 58 15713.0 59 12357.0 60 9488.0 61 7127.5 62 5225.5 63 3704.0 64 2600.5 65 1760.5 66 1103.0 67 679.5 68 421.0 69 292.0 70 191.0 71 119.0 72 84.0 73 60.5 74 41.0 75 24.5 76 15.0 77 10.5 78 8.0 79 6.0 80 3.0 81 2.0 82 1.5 83 1.5 84 1.0 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 21.855653008949844 2 28.786237302066866 3 22.613816002294936 4 28.902815162039158 5 31.94635180471886 6 24.674070276307837 7 23.604066593830936 8 19.66106970867742 9 11.624165593458619 10-11 15.19741433154906 12-13 15.292731482329197 14-15 19.418021146776205 16-17 22.650386456623067 18-19 26.568231617624622 20-21 28.60803898949579 22-23 28.181146328611216 24-25 26.740911301135057 26-27 28.173923791038764 28-29 25.171662424697416 30-31 27.00468651135159 32-33 26.035874649300023 34-35 24.127675136363543 36-37 27.04667386885906 38-39 27.178815788670583 40-41 24.237895128754445 42-43 25.092214511400417 44-45 23.85894078943353 46-47 27.062822852515783 48-49 22.510360781010625 50-51 28.105309684100447 52-53 21.67758185373039 54-55 22.804704618558464 56-57 24.91251574208013 58-59 24.204834217331243 60-61 23.74818673616579 62-63 23.57614283941349 64-65 22.103915022267795 66-67 22.61516387022219 68-69 23.58606111284045 70-71 22.100024007307987 72-73 21.962744930490704 74-75 19.306758464101954 76-77 21.534733284911812 78-79 20.637434717415672 80-81 21.665222159152176 82-83 22.48820997035708 84-85 19.496934999196366 86-87 21.027909512794064 88-89 19.87100649621478 90-91 21.004003930688054 92-93 20.977631495960466 94-95 20.06047095014008 96-97 18.966256507913258 98-99 18.3619539100377 100-101 20.027130292543287 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1966068.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.4263564224958 #Duplication Level Percentage of deduplicated Percentage of total 1 92.63880060512703 77.28517597835848 2 5.116126087642097 8.536395169801171 3 1.1161812953628163 2.793568157371842 4 0.4377298030524839 1.4607281026482166 5 0.22412112101475715 0.9348804261793223 6 0.12086608105724432 0.6050050054603161 7 0.07891180646590579 0.46083271445173657 8 0.05584304716499931 0.37270255652043816 9 0.03569489350672168 0.2680105417339315 >10 0.16854222957605866 2.252283191514968 >50 0.005303431058207602 0.3027149927011908 >100 0.0016372300020247841 0.20323435188668498 >500 6.0592242428325744E-5 0.03371678338846939 >1k 1.2118448485665148E-4 0.1393660746656823 >5k 0.0 0.0 >10k+ 6.059224242832574E-5 4.351385953317591 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 86081 4.3783327941861625 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 5.086294065108633E-5 8 0.0 0.0 0.0 0.0 5.086294065108633E-5 9 0.0 0.0 0.0 0.0 5.086294065108633E-5 10-11 0.0 0.0 0.0 2.5431470325543164E-5 5.086294065108633E-5 12-13 0.0 0.0 0.0 5.086294065108633E-5 5.086294065108633E-5 14-15 0.0 0.0 0.0 5.086294065108633E-5 5.086294065108633E-5 16-17 0.0 0.0 0.0 5.086294065108633E-5 5.086294065108633E-5 18-19 0.0 0.0 0.0 5.086294065108633E-5 1.0172588130217266E-4 20-21 0.0 0.0 0.0 7.629441097662949E-5 1.0172588130217266E-4 22-23 0.0 0.0 0.0 1.0172588130217266E-4 1.0172588130217266E-4 24-25 0.0 0.0 0.0 1.0172588130217266E-4 1.0172588130217266E-4 26-27 0.0 0.0 0.0 1.0172588130217266E-4 1.0172588130217266E-4 28-29 0.0 0.0 0.0 2.797461735809748E-4 1.0172588130217266E-4 30-31 0.0 0.0 0.0 8.901014613940108E-4 1.0172588130217266E-4 32-33 0.0 0.0 0.0 0.003280659671995068 1.0172588130217266E-4 34-35 2.5431470325543164E-5 0.0 0.0 0.006383299051711335 1.0172588130217266E-4 36-37 5.086294065108633E-5 0.0 0.0 0.010986395180634647 1.0172588130217266E-4 38-39 5.086294065108633E-5 0.0 0.0 0.018336090104716624 1.0172588130217266E-4 40-41 5.086294065108633E-5 0.0 0.0 0.02619441443530946 1.0172588130217266E-4 42-43 5.086294065108633E-5 0.0 0.0 0.03402730729557675 1.0172588130217266E-4 44-45 5.086294065108633E-5 0.0 0.0 0.044530504540026085 1.0172588130217266E-4 46-47 5.086294065108633E-5 0.0 0.0 0.0575005544060531 1.0172588130217266E-4 48-49 5.086294065108633E-5 0.0 0.0 0.07036887839077793 1.0172588130217266E-4 50-51 5.086294065108633E-5 0.0 0.0 0.08679760822107882 1.2715735162771582E-4 52-53 5.086294065108633E-5 0.0 0.0 0.10726994183314106 1.5258882195325898E-4 54-55 5.086294065108633E-5 0.0 0.0 0.12807288455943538 1.5258882195325898E-4 56-57 5.086294065108633E-5 0.0 0.0 0.15431816193539594 1.5258882195325898E-4 58-59 5.086294065108633E-5 0.0 0.0 0.18132638342112278 1.5258882195325898E-4 60-61 5.086294065108633E-5 0.0 0.0 0.21041998547354415 1.5258882195325898E-4 62-63 5.086294065108633E-5 0.0 0.0 0.24253993249470518 1.5258882195325898E-4 64-65 5.086294065108633E-5 0.0 0.0 0.27435470187195965 1.5258882195325898E-4 66-67 5.086294065108633E-5 0.0 0.0 0.31163723736920595 1.5258882195325898E-4 68-69 5.086294065108633E-5 0.0 0.0 0.35410779281286303 1.5258882195325898E-4 70-71 5.086294065108633E-5 0.0 0.0 0.40184266261390755 1.5258882195325898E-4 72-73 5.086294065108633E-5 0.0 0.0 0.4493486491820222 1.5258882195325898E-4 74-75 5.086294065108633E-5 0.0 0.0 0.4988382904355292 1.5258882195325898E-4 76-77 5.086294065108633E-5 0.0 0.0 0.5527275760553552 1.5258882195325898E-4 78-79 7.629441097662949E-5 0.0 0.0 0.6153398559968424 1.5258882195325898E-4 80-81 1.0172588130217266E-4 0.0 0.0 0.68428457204939 1.5258882195325898E-4 82-83 1.0172588130217266E-4 0.0 0.0 0.7571457345320711 1.5258882195325898E-4 84-85 1.0172588130217266E-4 0.0 0.0 0.8320922775814468 1.5258882195325898E-4 86-87 1.0172588130217266E-4 0.0 0.0 0.9117945055816992 1.5258882195325898E-4 88-89 1.0172588130217266E-4 0.0 0.0 0.9993804893828697 1.5258882195325898E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1595 0.0 58.75545 1 GTATCAA 3085 0.0 49.63337 1 ATCAACG 3905 0.0 38.301693 3 TCAACGC 3940 0.0 38.08782 4 CAACGCA 4015 0.0 37.369255 5 TATCAAC 4145 0.0 36.43802 2 AACGCAG 4395 0.0 34.39382 6 ACGCAGA 4780 0.0 31.016527 7 CGCAGAG 4880 0.0 29.858599 8 GCAGAGT 5250 0.0 25.281908 9 GTACATG 3150 0.0 25.198744 1 TACATGG 3175 0.0 23.54325 2 ACATGGG 3350 0.0 21.947779 3 AGAGTAC 4685 0.0 21.132212 10-11 GAGTACT 3545 0.0 21.131588 12-13 CAGAGTA 5060 0.0 20.924845 10-11 GTATAAC 515 0.0 19.887514 1 AGTACTT 3865 0.0 19.319891 12-13 CATGGGT 915 0.0 19.262516 4 GTGGTAT 885 0.0 19.095385 1 >>END_MODULE