FastQCFastQC Report
Sat 18 Jun 2016
SRR1931051_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931051_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966068
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57230.2910886093461671No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51130.2600622155490044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30520.15523369486711547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27100.061.2323531
GTATCAA53500.042.8369061
ATCAACG67200.033.8173183
TCAACGC67450.033.762464
CAACGCA68100.033.505535
TATCAAC68950.033.09862
AACGCAG71650.032.1756676
ACGCAGA82000.027.6509747
CGCAGAG82200.027.5259938
GTACATG49000.023.7735921
GCAGAGT94850.023.5548329
TACATGG49750.022.84062
ACATGGG47400.022.7681663
TGGTATC13300.021.4487672
AGAGTAC89800.020.8649410-11
CAGAGTA92700.020.59598510-11
GTGGTAT14800.020.5609461
GAGTACT73450.020.34300812-13
AGTACTT78200.018.98602312-13
CCGTCCA2803.547393E-818.6350739
Produced by FastQC (version 0.11.5)