##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931051_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1966068 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.347682786149818 31.0 31.0 34.0 30.0 34.0 2 31.50562238945957 31.0 31.0 34.0 30.0 34.0 3 31.568247893765626 31.0 31.0 34.0 30.0 34.0 4 35.3446193112344 37.0 35.0 37.0 33.0 37.0 5 35.22160779789916 37.0 35.0 37.0 33.0 37.0 6 35.250229900491746 37.0 35.0 37.0 32.0 37.0 7 35.1391782990212 37.0 35.0 37.0 32.0 37.0 8 35.14550971787344 37.0 35.0 37.0 32.0 37.0 9 36.64494819100865 39.0 35.0 39.0 32.0 39.0 10-11 36.589947550135605 38.5 35.0 39.0 32.0 39.0 12-13 36.47424071802196 38.0 35.0 39.0 32.0 39.0 14-15 37.52801047573126 39.0 36.0 41.0 32.0 41.0 16-17 37.48780001505543 39.0 36.0 41.0 32.0 41.0 18-19 37.525462242404636 39.0 36.0 41.0 32.0 41.0 20-21 37.51207257327824 39.0 36.0 41.0 32.0 41.0 22-23 37.412804134953625 39.0 36.0 41.0 32.0 41.0 24-25 37.285196646301145 39.0 36.0 41.0 31.5 41.0 26-27 37.029157689357646 39.0 36.0 40.0 31.0 41.0 28-29 36.8393382121066 39.0 36.0 40.0 30.5 41.0 30-31 36.60554339931274 38.5 35.0 40.0 30.0 41.0 32-33 36.42563253152994 38.0 35.0 40.0 30.0 41.0 34-35 36.48577210961167 38.0 35.0 40.0 30.0 41.0 36-37 36.535492159986326 38.5 35.0 40.0 30.0 41.0 38-39 36.50914591967317 39.0 35.0 40.0 30.0 41.0 40-41 36.38310933294271 39.0 35.0 40.0 30.0 41.0 42-43 36.235405896438984 38.5 35.0 40.0 30.0 41.0 44-45 36.06421090216615 38.0 35.0 40.0 29.5 41.0 46-47 35.81267916470844 38.0 35.0 40.0 28.5 41.0 48-49 35.58463644187282 38.0 35.0 40.0 27.5 41.0 50-51 35.32165749099217 38.0 34.0 40.0 27.0 41.0 52-53 35.11960573082925 38.0 34.0 40.0 26.0 41.0 54-55 34.967285973832034 38.0 34.0 40.0 26.0 41.0 56-57 34.80314541511281 38.0 34.0 40.0 26.0 41.0 58-59 34.60031112860796 38.0 33.5 40.0 26.0 41.0 60-61 34.29604265976558 37.5 33.0 40.0 25.0 41.0 62-63 34.05062897112409 37.0 33.0 40.0 24.5 41.0 64-65 33.75504534939789 37.0 32.5 40.0 23.5 41.0 66-67 33.437939582964574 36.0 32.0 39.0 23.0 41.0 68-69 33.09149937845487 36.0 32.0 39.0 22.0 40.0 70-71 32.712066164547714 35.5 31.0 39.0 22.0 40.0 72-73 32.29357758734693 35.0 31.0 38.0 21.5 40.0 74-75 31.778850985825514 35.0 31.0 37.5 20.0 39.5 76-77 30.40336626200111 33.5 29.0 36.0 19.0 39.0 78-79 31.126678477041487 34.0 30.0 36.0 20.0 39.0 80-81 31.084565233755903 34.0 30.0 36.0 20.0 38.5 82-83 30.80225989131607 34.0 30.0 36.0 19.0 37.0 84-85 30.44872125480909 34.0 30.0 35.0 18.0 37.0 86-87 30.091958416494244 34.0 30.0 35.0 16.5 36.5 88-89 29.736562011079982 34.0 29.5 35.0 11.5 36.0 90-91 29.389908945163647 34.0 29.0 35.0 7.0 36.0 92-93 29.057896776713726 34.0 29.0 35.0 2.0 35.5 94-95 28.6683644716256 34.0 29.0 35.0 2.0 35.0 96-97 28.231039312984088 34.0 29.0 35.0 2.0 35.0 98-99 27.73279637326888 33.5 28.5 35.0 2.0 35.0 100-101 26.592002412937905 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3107324135936693 1101 2 0.41750620834915964 1101 3 0.3298045751745313 1101 4 0.18232655839945977 1101 5 0.347122479365801 1101 6 0.38022755259900265 1101 7 0.3361250873799051 1101 8 0.40021454051519356 1101 9 0.468679328892307 1101 10-11 0.4653462286928729 1101 12-13 0.38592031005147476 1101 14-15 0.4503110309284821 1101 16-17 0.5648833786308032 1101 18-19 0.4387888810972882 1101 20-21 0.42020464969505866 1101 22-23 0.50358854617145 1101 24-25 0.4777325391138234 1101 26-27 0.5245713930780767 1101 28-29 0.6266428919829465 1101 30-31 0.6158086105530245 1101 32-33 0.5141529136882781 1101 34-35 0.5164972172209019 1101 36-37 0.471350103858434 1101 38-39 0.6178795480544892 1101 40-41 0.6101933334897112 1101 42-43 0.5454553321638471 1101 44-45 0.6239889384461534 1101 46-47 0.5133912914726224 1101 48-49 0.517404783646839 1101 50-51 0.4932282301951929 1101 52-53 0.4752776507112557 1101 54-55 0.6913809350110611 1101 56-57 0.6036855761273188 1101 58-59 0.45407509717291816 1101 60-61 0.2921804620207382 1101 62-63 0.10524934185058754 1101 64-65 0.14877133660362318 1101 66-67 0.09327075093300152 1101 68-69 0.08937712797558106 1101 70-71 0.19683203891140977 1101 72-73 0.13166378968640657 1101 74-75 0.12300386772036376 1101 76-77 0.1937659653401873 1101 78-79 0.3392588113117547 1101 80-81 0.3309868811493146 1101 82-83 0.25845849325068215 1101 84-85 0.3195761515847515 1101 86-87 0.19219412903118993 1101 88-89 0.31490970144279373 1101 90-91 0.3440271901499834 1101 92-93 0.28033384196320554 1101 94-95 0.3728265550807741 1101 96-97 0.5588954759079883 1101 98-99 0.578228351323343 1101 100-101 0.8887516670452911 1102 1 0.36936684659535857 1102 2 0.4296840171581451 1102 3 0.43676477765314914 1102 4 0.2193763634846988 1102 5 0.35555327007971727 1102 6 0.3057337491429024 1102 7 0.3959057355442184 1102 8 0.3500312624561914 1102 9 0.5399701300792472 1102 10-11 0.46583278830188135 1102 12-13 0.5194921957790655 1102 14-15 0.571692016557158 1102 16-17 0.5649604717826051 1102 18-19 0.45878437187520404 1102 20-21 0.5005088039977252 1102 22-23 0.5485214896752595 1102 24-25 0.5766226886454433 1102 26-27 0.6380176024223516 1102 28-29 0.5733104314022697 1102 30-31 0.51293143594431 1102 32-33 0.6483459083935728 1102 34-35 0.7836424432638438 1102 36-37 0.5864298152682181 1102 38-39 0.5459392734283242 1102 40-41 0.5135076117232629 1102 42-43 0.6279304587129744 1102 44-45 0.6853499962223779 1102 46-47 0.726714588150351 1102 48-49 0.5981547691009652 1102 50-51 0.45680535243875653 1102 52-53 0.45343919722896686 1102 54-55 0.48248176703526013 1102 56-57 0.4889215102635802 1102 58-59 0.527497596736545 1102 60-61 0.39334776870602184 1102 62-63 0.2799809704070526 1102 64-65 0.4443777542448615 1102 66-67 0.48239843464743615 1102 68-69 0.4660804502540472 1102 70-71 0.4801173708483404 1102 72-73 0.4738148340804216 1102 74-75 0.4435942848961396 1102 76-77 0.48750396499108817 1102 78-79 0.678833053471525 1102 80-81 0.597078985646899 1102 82-83 0.3061980638563746 1102 84-85 0.4502388617726858 1102 86-87 0.4474015532471647 1102 88-89 0.45907025884082486 1102 90-91 0.6389194051962406 1102 92-93 0.5711364834945947 1102 94-95 0.6780056148352394 1102 96-97 0.8117551954634656 1102 98-99 0.8478885596203121 1102 100-101 0.8976602898415891 1103 1 0.3295220951354523 1103 2 0.45208348542002597 1103 3 0.3124935437358545 1103 4 0.20242365019598907 1103 5 0.24522710571566364 1103 6 0.3806150575984901 1103 7 0.3858952906883104 1103 8 0.38501203442599774 1103 9 0.42376754735123967 1103 10-11 0.31512241940856 1103 12-13 0.3879226187957272 1103 14-15 0.512378291066959 1103 16-17 0.43857890220562723 1103 18-19 0.4393136174717469 1103 20-21 0.4264268830937681 1103 22-23 0.4319990332350585 1103 24-25 0.39362287855191624 1103 26-27 0.5181011612696196 1103 28-29 0.4503091970102062 1103 30-31 0.4247436184444311 1103 32-33 0.39850614197853673 1103 34-35 0.5262491469622717 1103 36-37 0.5459880453399037 1103 38-39 0.5449013158724156 1103 40-41 0.5011464808302293 1103 42-43 0.408092353979562 1103 44-45 0.47239065994549634 1103 46-47 0.48965493378012326 1103 48-49 0.4075551394185837 1103 50-51 0.4975996362539732 1103 52-53 0.492464383574692 1103 54-55 0.4779928528255155 1103 56-57 0.5393701643014666 1103 58-59 0.7489801728178236 1103 60-61 0.6706414828018268 1103 62-63 0.44622212788699045 1103 64-65 0.6008589060276037 1103 66-67 0.5636172781170217 1103 68-69 0.4841286864976979 1103 70-71 0.583240145381879 1103 72-73 0.6304259531043002 1103 74-75 0.632290695663837 1103 76-77 0.6306424068084944 1103 78-79 0.7446516690533507 1103 80-81 0.6959075001035302 1103 82-83 0.8638987712579755 1103 84-85 0.6423696123780118 1103 86-87 0.6852878942242349 1103 88-89 0.5819332175836465 1103 90-91 0.7193418284028112 1103 92-93 0.5244610336536404 1103 94-95 0.6286863108424576 1103 96-97 0.7400322690047467 1103 98-99 0.7549863139762927 1103 100-101 0.9219226218930565 1104 1 0.31634510930842197 1104 2 0.31757351221613916 1104 3 0.3832438930859645 1104 4 0.1828688868756032 1104 5 0.2869946020185452 1104 6 0.28943054838158133 1104 7 0.3023058448431044 1104 8 0.4138015086613578 1104 9 0.3499212753619645 1104 10-11 0.39294680305722807 1104 12-13 0.4725563334956604 1104 14-15 0.5219890551227024 1104 16-17 0.591253684887171 1104 18-19 0.5503847289810366 1104 20-21 0.4161042709206839 1104 22-23 0.5105998409581076 1104 24-25 0.45299599145047154 1104 26-27 0.5164659351432306 1104 28-29 0.5586175047361692 1104 30-31 0.580948320737626 1104 32-33 0.6262376176962263 1104 34-35 0.5287802917718167 1104 36-37 0.5652284226496604 1104 38-39 0.6398579918046678 1104 40-41 0.48188713796913163 1104 42-43 0.4935894085014496 1104 44-45 0.6755911839997495 1104 46-47 0.420743312085861 1104 48-49 0.52628321413588 1104 50-51 0.37946461316370517 1104 52-53 0.2717970521441231 1104 54-55 0.47583008185374354 1104 56-57 0.5029193054412389 1104 58-59 0.399190481002222 1104 60-61 0.36713099415381123 1104 62-63 0.4963059602780575 1104 64-65 0.5554585176410356 1104 66-67 0.4700369427176483 1104 68-69 0.3944524622015422 1104 70-71 0.3962613344405028 1104 72-73 0.33236323181530025 1104 74-75 0.39323352790713173 1104 76-77 0.3960540116315592 1104 78-79 0.6074833771281405 1104 80-81 0.5232647843296618 1104 82-83 0.5045590516921585 1104 84-85 0.5690038785915199 1104 86-87 0.5150161050746256 1104 88-89 0.7999773689217662 1104 90-91 0.5734501973304909 1104 92-93 0.5308812800196456 1104 94-95 0.6089403794186303 1104 96-97 0.6087965877051289 1104 98-99 0.7005734490494113 1104 100-101 0.9360777880277951 1105 1 0.2298002244919708 1105 2 0.22489903498345498 1105 3 0.2687222197208854 1105 4 0.14014285189824705 1105 5 0.1113242148212521 1105 6 0.19422528211973145 1105 7 0.3762588995370848 1105 8 0.3155225726166222 1105 9 0.24290573613985345 1105 10-11 0.27356730256212813 1105 12-13 0.34540574301614413 1105 14-15 0.39708473046089665 1105 16-17 0.37657199139045616 1105 18-19 0.3322330126951627 1105 20-21 0.36272471308863885 1105 22-23 0.2357457543811492 1105 24-25 0.4110561779502504 1105 26-27 0.40343092684302206 1105 28-29 0.4382001372846318 1105 30-31 0.37345672560562804 1105 32-33 0.44017968735970925 1105 34-35 0.37960389602676514 1105 36-37 0.5025115888867546 1105 38-39 0.6047210247652188 1105 40-41 0.29215623026158966 1105 42-43 0.37606437136894755 1105 44-45 0.3822194258124014 1105 46-47 0.5007954705032915 1105 48-49 0.3991045240029223 1105 50-51 0.4996139531517656 1105 52-53 0.4900729984767409 1105 54-55 0.4843394987643208 1105 56-57 0.48527010652026803 1105 58-59 0.4431420931714882 1105 60-61 0.4424676439288362 1105 62-63 0.4751653509774627 1105 64-65 0.5624481642270354 1105 66-67 0.6759423294780902 1105 68-69 0.47260340034317494 1105 70-71 0.49110778974316815 1105 72-73 0.48106750787721353 1105 74-75 0.6104670567321442 1105 76-77 0.47270895607843144 1105 78-79 0.5292007005303496 1105 80-81 0.6932688252190928 1105 82-83 0.6341420255319541 1105 84-85 0.5614131041969266 1105 86-87 0.6245405906803221 1105 88-89 0.6676264085734473 1105 90-91 0.6912812590286812 1105 92-93 0.6693901599651859 1105 94-95 0.8225549686676814 1105 96-97 0.7893259396702383 1105 98-99 0.962714205787865 1105 100-101 1.0409145233531767 1106 1 0.2766788030264884 1106 2 0.20717343522107257 1106 3 0.2861601947713446 1106 4 0.07145824961656899 1106 5 0.11768058675981052 1106 6 0.09591801841036585 1106 7 0.22253377929833107 1106 8 0.1718379256032634 1106 9 0.21725323510207772 1106 10-11 0.23970709711846183 1106 12-13 0.3262901062517045 1106 14-15 0.35342694482227444 1106 16-17 0.41452492203129765 1106 18-19 0.3654835253202364 1106 20-21 0.26791747979681446 1106 22-23 0.16202827878674952 1106 24-25 0.29067304662519433 1106 26-27 0.30751568980090127 1106 28-29 0.38991295810024695 1106 30-31 0.2644703320885 1106 32-33 0.398780068831492 1106 34-35 0.3554266458442612 1106 36-37 0.4509844940126513 1106 38-39 0.4569068607466633 1106 40-41 0.35465108611821705 1106 42-43 0.3367002680065596 1106 44-45 0.29226356703568257 1106 46-47 0.3036882236130367 1106 48-49 0.29914118161590153 1106 50-51 0.27412221777724 1106 52-53 0.4674628109257455 1106 54-55 0.5251693474996628 1106 56-57 0.5423100456494012 1106 58-59 0.6294879039600474 1106 60-61 0.6501089781496461 1106 62-63 0.44898758816989215 1106 64-65 0.39316916736183316 1106 66-67 0.13563796617095392 1106 68-69 0.3457830445652661 1106 70-71 0.34247045095614936 1106 72-73 0.37523100754082606 1106 74-75 0.586800195035341 1106 76-77 0.6249952817823292 1106 78-79 0.5587383717632264 1106 80-81 0.4640932122161274 1106 82-83 0.4764144262634069 1106 84-85 0.40940277296776983 1106 86-87 0.4517778904613152 1106 88-89 0.5518491866613644 1106 90-91 0.47042752804859234 1106 92-93 0.4335525458099312 1106 94-95 0.6885957720128957 1106 96-97 0.7766620651551328 1106 98-99 0.7838560311625393 1106 100-101 0.9689750202684806 1107 1 0.1261413887249745 1107 2 0.20287585630548577 1107 3 0.08926655556638607 1107 4 -0.046051998156713125 1107 5 0.05696913553093452 1107 6 0.216996714055675 1107 7 0.20180794569817806 1107 8 0.094704449677387 1107 9 0.22101503508788767 1107 10-11 0.2348482630739852 1107 12-13 0.22471371915054306 1107 14-15 0.12118658122239623 1107 16-17 0.2132945439889724 1107 18-19 0.19938371335460658 1107 20-21 0.3122041250842358 1107 22-23 0.22692249916054408 1107 24-25 0.31596790859871504 1107 26-27 0.4170834821892342 1107 28-29 0.4079633457945917 1107 30-31 0.3784528494084256 1107 32-33 0.32048369897848517 1107 34-35 0.44639469913557406 1107 36-37 0.43012237943472087 1107 38-39 0.42529112014910453 1107 40-41 0.2902549309995379 1107 42-43 0.27774389327192495 1107 44-45 0.46735347354359646 1107 46-47 0.4599064965670365 1107 48-49 0.38300971908849846 1107 50-51 0.33074668591986267 1107 52-53 0.41150272645601405 1107 54-55 0.3684473355518847 1107 56-57 0.5044683224882363 1107 58-59 0.40757307723491465 1107 60-61 0.36991406408898797 1107 62-63 0.2981421232337027 1107 64-65 0.09212315245124358 1107 66-67 0.13957231456208774 1107 68-69 0.3748864314312499 1107 70-71 0.2803383400650361 1107 72-73 0.40131107044590664 1107 74-75 0.4430030710201045 1107 76-77 0.47999031892974386 1107 78-79 0.5607450952547985 1107 80-81 0.5491037286576201 1107 82-83 0.517938294569479 1107 84-85 0.612250166530103 1107 86-87 0.5208547687302492 1107 88-89 0.4500000151050827 1107 90-91 0.37776069065060014 1107 92-93 0.41612115187914256 1107 94-95 0.6472833895343797 1107 96-97 0.6715300239063815 1107 98-99 0.6945022121467943 1107 100-101 0.9513473817296898 1108 1 0.08457644277054754 1108 2 0.06656611397364998 1108 3 0.09201891123691652 1108 4 0.02531349886134393 1108 5 0.0875661332801485 1108 6 0.13280694416837946 1108 7 0.12029601415203217 1108 8 0.03715952450121307 1108 9 0.1716706622514792 1108 10-11 0.14131693696860737 1108 12-13 0.16263371944223337 1108 14-15 0.24217993596294463 1108 16-17 0.14260141204841403 1108 18-19 0.18321413652416396 1108 20-21 0.18825070046604253 1108 22-23 0.021928174555306157 1108 24-25 0.019019313478246147 1108 26-27 0.104191938629981 1108 28-29 0.1573658968395364 1108 30-31 0.16349918118464046 1108 32-33 0.2528487400051773 1108 34-35 0.18881973914590588 1108 36-37 0.1676038668917741 1108 38-39 0.23500804317134083 1108 40-41 0.19408439250814524 1108 42-43 0.18206546540070434 1108 44-45 0.11029343513640555 1108 46-47 0.15753682270455016 1108 48-49 0.25716043232416297 1108 50-51 0.12457478241683617 1108 52-53 0.011743004963967962 1108 54-55 -0.022884065800049314 1108 56-57 -0.03470181424764007 1108 58-59 -0.10061539737256453 1108 60-61 0.05171276201829045 1108 62-63 -0.1637969544058322 1108 64-65 -0.06489933104499102 1108 66-67 -0.2244354963124664 1108 68-69 -0.15182415716221698 1108 70-71 0.16768602675772826 1108 72-73 -0.09682178050736212 1108 74-75 0.01850997686232958 1108 76-77 0.1415471933128991 1108 78-79 0.1996016439265773 1108 80-81 0.13472163341718257 1108 82-83 0.21042840621102243 1108 84-85 0.15345608480122763 1108 86-87 0.09727775917220427 1108 88-89 0.16637019623232874 1108 90-91 0.31606410197190016 1108 92-93 0.19498960965071532 1108 94-95 0.1688658413641697 1108 96-97 0.4616940098861875 1108 98-99 0.5646904037251517 1108 100-101 0.8569103396438038 1109 1 0.11583855049740421 1109 2 0.16236468544252958 1109 3 0.1641467261651286 1109 4 0.04125389116718026 1109 5 0.07335652224828948 1109 6 0.11469464502330595 1109 7 0.18444365426789489 1109 8 0.1155738948718934 1109 9 0.28253769764681635 1109 10-11 0.040522842132801884 1109 12-13 0.0997351322139508 1109 14-15 0.044146901782937675 1109 16-17 0.24356686455308818 1109 18-19 0.08778787227962681 1109 20-21 0.1915649917644373 1109 22-23 0.11855084565663532 1109 24-25 0.004944586394586281 1109 26-27 -0.06635023290523634 1109 28-29 0.1135127854591147 1109 30-31 0.23184198736259987 1109 32-33 0.14714308017836686 1109 34-35 0.03584379322061437 1109 36-37 0.2483862763732887 1109 38-39 0.25443821354490836 1109 40-41 0.17500072248108012 1109 42-43 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-1.1553647007843644 2316 84-85 -1.0429505069980785 2316 86-87 -0.9265089411831617 2316 88-89 -1.108927754317925 2316 90-91 -0.9700156804659308 2316 92-93 -1.033092882670477 2316 94-95 -1.343277200616523 2316 96-97 -1.3323591944785598 2316 98-99 -1.3674980601438733 2316 100-101 -1.6757461657639077 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 11.0 4 19.0 5 44.0 6 91.0 7 277.0 8 675.0 9 1361.0 10 2355.0 11 3743.0 12 5621.0 13 7488.0 14 9180.0 15 11334.0 16 13145.0 17 15417.0 18 13090.0 19 10127.0 20 9672.0 21 10293.0 22 11237.0 23 13050.0 24 16115.0 25 20566.0 26 26340.0 27 33798.0 28 43092.0 29 53475.0 30 66106.0 31 80195.0 32 96050.0 33 117112.0 34 144140.0 35 181135.0 36 236978.0 37 327160.0 38 323299.0 39 62252.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.11666469987426 21.774884332260413 15.197184502760969 22.911266465104354 2 14.73874447600368 22.289681215564038 41.06796244903274 21.903611859399543 3 18.004589955809468 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20.374865626229454 27.95902677658846 31.094478334764702 20.57162926241738 24-25 20.317194132503648 28.15218726467745 31.18781476477091 20.342803838047985 26-27 20.20953553253663 28.36160965201181 31.012530423216507 20.416324392235055 28-29 20.252250309374624 28.55747755794194 30.96129167783535 20.228980454848088 30-31 20.663823925719107 28.18774501600504 30.962316811830252 20.186114246445598 32-33 20.424377746507822 28.39297687617199 30.74079274428467 20.441852633035523 34-35 20.532897619998998 28.434898745738735 30.899749900613916 20.13245373364835 36-37 20.590800162051185 28.279050510275578 30.91263655902165 20.21751276865159 38-39 20.382872343672116 28.545670020243534 30.90485539607133 20.16660224001302 40-41 20.590155412610574 28.419711961880434 30.7727599528897 20.217372672619287 42-43 20.533798542516383 28.37942186957933 30.52413861863597 20.562640969268315 44-45 20.43753980316635 28.728424903505932 30.479611622829772 20.354423670497944 46-47 20.626114009457382 28.670610294057802 30.064266646048164 20.639009050436652 48-49 20.61405460947902 28.797922545644607 30.035925615536147 20.552097229340227 50-51 20.691229574754367 28.991447085642015 29.776570431411457 20.54075290819216 52-53 20.81375984381775 29.041308708693265 29.618513951736364 20.526417495752625 54-55 20.66707007595846 28.9738168038538 29.821078059189247 20.538035060998485 56-57 20.68652076205707 29.13028461673844 29.580577656919687 20.602616964284806 58-59 20.675172562724008 29.06544602030437 29.76844024460068 20.49094117237094 60-61 20.6931154164531 29.18421976646452 29.770159106516346 20.352505710566028 62-63 20.653347622067734 29.078279764174702 29.807713877270515 20.46065873648705 64-65 20.469813970574553 28.953931504114937 29.831830908575025 20.744423616735485 66-67 20.507531851404643 29.10445363865266 29.622979107028673 20.765035402914023 68-69 20.53805657178549 29.1265153156828 29.718058866269804 20.6173692462619 70-71 20.661896881694446 28.826457522097726 29.700960584528076 20.81068501167975 72-73 20.596035531564905 29.117462316357674 29.54711365892897 20.739388493148454 74-75 20.571614910664614 29.256057718833322 29.444294584703528 20.72803278579853 76-77 20.519343844561234 29.11671285236503 29.50023889154324 20.8637044115305 78-79 20.41883365349083 29.210444060521084 29.522454557176786 20.8482677288113 80-81 20.57553001781222 29.25758850414991 29.351405293616793 20.815476184421073 82-83 20.680490196676818 29.258067371016665 29.401297411890127 20.660145020416383 84-85 20.737863593731245 29.279150059916542 29.189911030544213 20.793075315808 86-87 20.560504519680904 29.416658019966757 29.215520521162038 20.807316939190304 88-89 20.610300114619783 29.292869438988685 29.229824743885697 20.867005702505843 90-91 20.629963234172077 29.24157509120245 29.33776239142687 20.790699283198606 92-93 20.652810356571827 29.24781266358285 29.26671133162101 20.832665648224314 94-95 20.450066517617696 29.282909308422777 29.247100933077757 21.01992324088177 96-97 20.423561245989774 29.152686771944502 29.33574254190154 21.088009440164186 98-99 20.309038141101933 29.171371488676893 29.22297194196742 21.29661842825375 100-101 20.327501388558282 29.223811180488163 29.243164529405902 21.205522901547656 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 163.0 1 134.5 2 156.0 3 241.0 4 215.5 5 195.5 6 375.0 7 808.5 8 1358.5 9 1719.5 10 1573.0 11 1268.5 12 1317.5 13 1524.0 14 1750.0 15 1978.5 16 2249.0 17 2594.0 18 2944.0 19 3305.5 20 3842.5 21 4576.5 22 5637.5 23 7346.5 24 9461.5 25 12030.5 26 15824.5 27 20296.5 28 25013.5 29 30247.5 30 35763.5 31 41479.5 32 48066.5 33 53609.0 34 57962.5 35 62394.5 36 68148.0 37 74012.0 38 76616.5 39 77495.0 40 78083.5 41 79189.0 42 79855.5 43 80487.5 44 81004.5 45 81493.0 46 80894.5 47 78611.0 48 76098.5 49 72089.5 50 67118.0 51 61203.5 52 55406.5 53 50185.0 54 43917.5 55 36437.5 56 29214.0 57 22760.0 58 17178.0 59 12831.0 60 9218.0 61 6525.0 62 4531.0 63 2764.0 64 1515.0 65 828.0 66 417.5 67 242.5 68 139.5 69 75.0 70 51.0 71 27.5 72 14.0 73 15.0 74 14.0 75 8.5 76 4.5 77 2.5 78 1.0 79 1.0 80 1.0 81 0.5 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004679390539899943 2 0.005086294065108633 3 0.0018310658634391077 4 0.16957704413072183 5 0.0018310658634391077 6 6.10355287813036E-4 7 0.0 8 6.10355287813036E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.7802029227880216E-4 24-25 8.392385207429244E-4 26-27 0.0026957358545075757 28-29 2.5431470325543165E-4 30-31 0.006357867581385792 32-33 0.019836546853923668 34-35 0.012690303692446038 36-37 0.0011444161646494424 38-39 4.0690352520869063E-4 40-41 0.002212537918322255 42-43 0.010884669299332475 44-45 0.007324263453756431 46-47 0.009969136367612921 48-49 0.010198019600542809 50-51 0.18501394661832654 52-53 0.11233080442792417 54-55 0.01640329835997534 56-57 0.006408730522036878 58-59 0.01696279070713729 60-61 0.12123181904186427 62-63 0.23478333404541452 64-65 0.2410140442751726 66-67 0.2507542974098556 68-69 0.2463292215732111 70-71 0.23162983172504717 72-73 0.156708720145997 74-75 0.2530431297391545 76-77 0.19737364119654052 78-79 0.005518629060642867 80-81 1.0172588130217266E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.2715735162771582E-4 90-91 0.005696649352921669 92-93 0.016327003948998713 94-95 0.0024159896809266006 96-97 2.5431470325543164E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1966068.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.97717306206734 #Duplication Level Percentage of deduplicated Percentage of total 1 78.31900035027202 50.106282394574656 2 13.658083590718121 17.476111551591107 3 3.7963798033082994 7.286449430567768 4 1.5089551447157066 3.861547377454945 5 0.7861629668130812 2.51482420913944 6 0.4732640964634314 1.816685940210233 7 0.2965416984507539 1.3280329693332287 8 0.20491373639133273 1.0487841260722512 9 0.14753305690349502 0.8494873122501656 >10 0.7439617084694172 8.52000450489404 >50 0.04438402256685133 1.9226978065153943 >100 0.019942826647948664 2.278431409318021 >500 5.580898142777919E-4 0.2178515598117748 >1k 1.594542326507941E-4 0.22009587464008196 >5k 1.594542326507941E-4 0.5527135336268663 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5723 0.2910886093461671 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5113 0.2600622155490044 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3052 0.15523369486711547 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 5.086294065108633E-5 0.0 20-21 0.0 0.0 0.0 5.086294065108633E-5 0.0 22-23 0.0 0.0 0.0 5.086294065108633E-5 0.0 24-25 0.0 0.0 0.0 1.5258882195325898E-4 0.0 26-27 0.0 0.0 0.0 2.0345176260434531E-4 0.0 28-29 0.0 0.0 0.0 6.103552878130359E-4 0.0 30-31 0.0 0.0 0.0 0.002034517626043453 0.0 32-33 0.0 0.0 0.0 0.006841065517571111 0.0 34-35 0.0 0.0 0.0 0.01324979603960799 0.0 36-37 0.0 0.0 0.0 0.022608577119407874 0.0 38-39 0.0 0.0 0.0 0.03896101253873213 0.0 40-41 0.0 0.0 0.0 0.05742425999507647 0.0 42-43 0.0 0.0 0.0 0.07583664451076971 0.0 44-45 0.0 0.0 0.0 0.09961506926515257 0.0 46-47 0.0 0.0 0.0 0.12794572720780767 0.0 48-49 0.0 0.0 0.0 0.15747166425576328 0.0 50-51 0.0 0.0 0.0 0.19198216948752533 0.0 52-53 0.0 0.0 0.0 0.22791683705751786 0.0 54-55 0.0 0.0 0.0 0.26741191047308643 0.0 56-57 0.0 0.0 0.0 0.31496875998185214 0.0 58-59 0.0 0.0 0.0 0.3660605838658683 0.0 60-61 0.0 0.0 0.0 0.4202550471296008 0.0 62-63 0.0 0.0 0.0 0.48251128648653047 0.0 64-65 0.0 0.0 0.0 0.5444623481995536 0.0 66-67 0.0 0.0 0.0 0.617094627449305 0.0 68-69 0.0 0.0 0.0 0.7011456368752251 0.0 70-71 0.0 0.0 0.0 0.7911221788869968 2.5431470325543164E-5 72-73 0.0 0.0 0.0 0.8847099896849957 5.086294065108633E-5 74-75 0.0 0.0 0.0 0.9837401351326608 5.086294065108633E-5 76-77 0.0 0.0 0.0 1.0951299751585397 5.086294065108633E-5 78-79 0.0 0.0 0.0 1.221041184740304 5.086294065108633E-5 80-81 0.0 0.0 0.0 1.3555227998217763 5.086294065108633E-5 82-83 0.0 0.0 0.0 1.5060771041489918 5.086294065108633E-5 84-85 0.0 0.0 0.0 1.6636759257563827 5.086294065108633E-5 86-87 0.0 0.0 0.0 1.8317270816675721 5.086294065108633E-5 88-89 0.0 0.0 0.0 2.0179108759208737 5.086294065108633E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2710 0.0 61.232353 1 GTATCAA 5350 0.0 42.836906 1 ATCAACG 6720 0.0 33.817318 3 TCAACGC 6745 0.0 33.76246 4 CAACGCA 6810 0.0 33.50553 5 TATCAAC 6895 0.0 33.0986 2 AACGCAG 7165 0.0 32.175667 6 ACGCAGA 8200 0.0 27.650974 7 CGCAGAG 8220 0.0 27.525993 8 GTACATG 4900 0.0 23.773592 1 GCAGAGT 9485 0.0 23.554832 9 TACATGG 4975 0.0 22.8406 2 ACATGGG 4740 0.0 22.768166 3 TGGTATC 1330 0.0 21.448767 2 AGAGTAC 8980 0.0 20.86494 10-11 CAGAGTA 9270 0.0 20.595985 10-11 GTGGTAT 1480 0.0 20.560946 1 GAGTACT 7345 0.0 20.343008 12-13 AGTACTT 7820 0.0 18.986023 12-13 CCGTCCA 280 3.547393E-8 18.635073 9 >>END_MODULE