FastQCFastQC Report
Sat 18 Jun 2016
SRR1931050_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931050_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2592308
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1167044.501934183746684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17950.057.6637731
GTATCAA34200.043.599651
TCAACGC41700.035.7506944
ATCAACG42250.035.409653
CAACGCA42850.034.6696625
TATCAAC43700.033.8779532
AACGCAG46750.033.134516
ACGCAGA53550.027.8419637
CGCAGAG53500.027.604538
GTACATG37650.024.7698021
GCAGAGT60300.023.0583959
TACATGG37650.022.7222982
ACATGGG37600.021.7029633
GTGTAGC9050.021.5154861
CATGGGG23600.020.487464
GAGTACT38450.020.2353512-13
CAGAGTA57050.019.50877810-11
AGAGTAC51100.019.36030810-11
GTATAAC7050.018.1705061
AGTACTT43300.017.19237512-13
Produced by FastQC (version 0.11.5)