##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931050_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2592308 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.140604434349623 28.0 2.0 31.0 2.0 33.0 2 18.9916757576646 26.0 2.0 31.0 2.0 33.0 3 19.56617153517252 26.0 2.0 31.0 2.0 33.0 4 21.098359068444026 28.0 2.0 35.0 2.0 37.0 5 20.496554421773954 28.0 2.0 35.0 2.0 37.0 6 21.320726935225288 28.0 2.0 35.0 2.0 37.0 7 21.44033926524163 28.0 2.0 35.0 2.0 37.0 8 21.77510079820762 29.0 2.0 35.0 2.0 37.0 9 22.897199329709277 30.0 2.0 37.0 2.0 39.0 10-11 23.131810340437944 31.0 2.0 37.0 2.0 39.0 12-13 22.967279351064768 30.5 2.0 37.0 2.0 39.0 14-15 22.89736848399187 30.5 2.0 37.5 2.0 40.0 16-17 21.873640400754848 28.0 2.0 37.0 2.0 39.0 18-19 20.939208419678526 27.0 2.0 37.0 2.0 39.0 20-21 20.607245743947093 26.0 2.0 36.5 2.0 39.0 22-23 20.26409188260037 25.0 2.0 36.0 2.0 39.0 24-25 20.073145243543593 25.0 2.0 36.0 2.0 39.0 26-27 19.775477296679252 24.0 2.0 36.0 2.0 39.0 28-29 19.53071760762996 23.0 2.0 36.0 2.0 39.0 30-31 19.43160361345951 23.5 2.0 36.0 2.0 39.0 32-33 19.268660205500275 22.5 2.0 35.5 2.0 39.0 34-35 18.913023452460124 19.0 2.0 35.0 2.0 38.5 36-37 18.49477704809768 16.5 2.0 35.0 2.0 38.0 38-39 18.303879593011324 16.0 2.0 34.5 2.0 38.0 40-41 18.1031852696516 16.0 2.0 34.0 2.0 38.0 42-43 17.91926557338094 15.5 2.0 34.0 2.0 38.0 44-45 17.527841791947562 14.5 2.0 34.0 2.0 38.0 46-47 17.187233538607295 9.0 2.0 33.0 2.0 38.0 48-49 16.885153114521884 9.0 2.0 33.0 2.0 38.0 50-51 14.920667991612108 5.5 2.0 30.5 2.0 37.0 52-53 16.68334588328239 9.0 2.0 33.0 2.0 37.5 54-55 17.30616365802212 9.0 2.0 34.0 2.0 38.0 56-57 17.222681679800395 8.0 2.0 34.0 2.0 38.0 58-59 16.938423983569855 7.5 2.0 34.0 2.0 38.0 60-61 16.63766863351114 4.0 2.0 34.0 2.0 38.0 62-63 16.19115533339403 2.0 2.0 33.0 2.0 38.0 64-65 15.815828211771132 2.0 2.0 33.0 2.0 38.0 66-67 15.42804635868886 2.0 2.0 32.0 2.0 37.5 68-69 14.972425923154194 2.0 2.0 31.5 2.0 37.0 70-71 14.593460537868186 2.0 2.0 31.0 2.0 36.0 72-73 14.181225186204726 2.0 2.0 31.0 2.0 36.0 74-75 13.719462540716613 2.0 2.0 30.0 2.0 35.0 76-77 13.284919654608943 2.0 2.0 29.5 2.0 35.0 78-79 12.881788545188304 2.0 2.0 29.0 2.0 34.0 80-81 12.434912826716578 2.0 2.0 29.0 2.0 34.0 82-83 11.966343505478516 2.0 2.0 27.5 2.0 34.0 84-85 11.540030544210024 2.0 2.0 26.0 2.0 34.0 86-87 11.059817159072146 2.0 2.0 26.0 2.0 33.5 88-89 10.63187649770012 2.0 2.0 25.5 2.0 33.0 90-91 10.152239240090298 2.0 2.0 24.5 2.0 32.0 92-93 9.63014483618459 2.0 2.0 20.5 2.0 32.0 94-95 9.082204930895557 2.0 2.0 15.0 2.0 32.0 96-97 8.487376307136344 2.0 2.0 4.5 2.0 31.5 98-99 7.872053205097542 2.0 2.0 2.0 2.0 31.0 100-101 7.091324796281923 2.0 2.0 2.0 2.0 30.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 8.405118513982053 1101 2 9.656062048486586 1101 3 9.158993843880136 1101 4 11.002578050145779 1101 5 11.86824706043814 1101 6 11.376098539822017 1101 7 10.779607467798485 1101 8 10.34458163585715 1101 9 10.299730088044019 1101 10-11 9.801974478136774 1101 12-13 9.809550891625658 1101 14-15 10.475525417893156 1101 16-17 11.375234853957853 1101 18-19 12.143843484580383 1101 20-21 12.152826390236996 1101 22-23 12.277650113445588 1101 24-25 12.130369579859536 1101 26-27 12.181403921454187 1101 28-29 12.4271421303804 1101 30-31 11.830985950106193 1101 32-33 11.649802472682047 1101 34-35 11.477429463971994 1101 36-37 11.984193705156613 1101 38-39 11.700248067269861 1101 40-41 11.171529636980157 1101 42-43 11.081034105948742 1101 44-45 10.851142911408825 1101 46-47 10.446312725766592 1101 48-49 10.558034457129597 1101 50-51 12.095579146440256 1101 52-53 11.47035215627282 1101 54-55 11.201167573460001 1101 56-57 11.37742218436411 1101 58-59 11.142068825757402 1101 60-61 10.624632091052913 1101 62-63 10.770546520314864 1101 64-65 10.652367808217102 1101 66-67 10.121617542370876 1101 68-69 9.963641838714716 1101 70-71 10.123984746051782 1101 72-73 9.959155095695166 1101 74-75 9.492537870656609 1101 76-77 9.342802209998306 1101 78-79 8.951617253507122 1101 80-81 9.027210452456588 1101 82-83 8.676788245869746 1101 84-85 8.061229026576799 1101 86-87 7.614027385114371 1101 88-89 7.113971282554838 1101 90-91 6.893248779570232 1101 92-93 6.617560742178858 1101 94-95 6.4232906923464785 1101 96-97 6.184689471207776 1101 98-99 5.493208130974036 1101 100-101 4.964191990605645 1102 1 7.699223861099814 1102 2 8.898973488051158 1102 3 8.577852694300976 1102 4 10.328247800798952 1102 5 10.987267343637527 1102 6 10.339121723561568 1102 7 10.103678998149608 1102 8 9.730911721742846 1102 9 9.422285963860094 1102 10-11 8.882636057224968 1102 12-13 9.110981191412765 1102 14-15 9.411564316272528 1102 16-17 10.074300157573479 1102 18-19 11.114206833612545 1102 20-21 11.101349557719928 1102 22-23 11.017312986799901 1102 24-25 10.74405321516344 1102 26-27 11.190754688453772 1102 28-29 10.992468266651503 1102 30-31 11.088845059738198 1102 32-33 10.875580993591502 1102 34-35 10.63986456050802 1102 36-37 10.750373821422816 1102 38-39 10.726133389743168 1102 40-41 10.27105665862593 1102 42-43 9.579050200904145 1102 44-45 9.706669299493583 1102 46-47 9.861154026785929 1102 48-49 9.273492555450272 1102 50-51 11.01278399694044 1102 52-53 10.495754254242712 1102 54-55 10.3204573587204 1102 56-57 10.3607489255607 1102 58-59 10.250373009019999 1102 60-61 10.364946422626655 1102 62-63 10.372090482260637 1102 64-65 9.968209383958515 1102 66-67 9.697553290215977 1102 68-69 9.351326966645422 1102 70-71 8.991825752662116 1102 72-73 9.0198428733069 1102 74-75 8.524499070115276 1102 76-77 8.4273156402092 1102 78-79 8.051851289275875 1102 80-81 7.560177773057253 1102 82-83 7.511587211053419 1102 84-85 7.192467805402858 1102 86-87 6.927473494694775 1102 88-89 6.79853507848018 1102 90-91 6.381068315501704 1102 92-93 6.089546920168338 1102 94-95 5.906140025630892 1102 96-97 5.493953824541533 1102 98-99 5.125554597573828 1102 100-101 4.576310366291221 1103 1 7.9936497988607975 1103 2 9.15139082538872 1103 3 8.515785236968465 1103 4 10.54063791540214 1103 5 11.158508523113397 1103 6 10.304341098456703 1103 7 10.0961870905033 1103 8 9.647412959396942 1103 9 9.714332740209496 1103 10-11 9.210669063847785 1103 12-13 9.127365733273376 1103 14-15 9.697421879536169 1103 16-17 10.541424558305703 1103 18-19 11.34085023652975 1103 20-21 11.658650208690162 1103 22-23 11.639069682668794 1103 24-25 11.446531728244114 1103 26-27 11.444496400507372 1103 28-29 11.13323563104213 1103 30-31 11.153749789594034 1103 32-33 11.086859764571763 1103 34-35 10.958937064603681 1103 36-37 11.085687589829735 1103 38-39 10.648573104771664 1103 40-41 10.351938720465274 1103 42-43 10.043399541733155 1103 44-45 9.983473041299899 1103 46-47 10.005535378835834 1103 48-49 10.082962635893072 1103 50-51 11.520888604633312 1103 52-53 10.90895256733809 1103 54-55 10.638901138306746 1103 56-57 10.41825883716222 1103 58-59 10.038317522903782 1103 60-61 10.107440847056228 1103 62-63 10.116969723794538 1103 64-65 9.855236921526457 1103 66-67 9.649224453158366 1103 68-69 9.415812232623223 1103 70-71 9.21645855681113 1103 72-73 8.93652509312859 1103 74-75 8.494715249544015 1103 76-77 8.225590276878075 1103 78-79 8.10223879848994 1103 80-81 7.784307220552632 1103 82-83 7.4301084736302645 1103 84-85 7.201156834678608 1103 86-87 6.9007739118963745 1103 88-89 6.536156181000921 1103 90-91 6.4389873222112435 1103 92-93 6.076586900260299 1103 94-95 5.782517893774072 1103 96-97 5.342876132290398 1103 98-99 4.637403273858942 1103 100-101 4.03383045813967 1104 1 8.204519086638406 1104 2 9.632406727014978 1104 3 8.81606786133274 1104 4 10.937231102732564 1104 5 11.463944389814994 1104 6 10.823896264461272 1104 7 10.394323186475269 1104 8 9.959736213754372 1104 9 9.801395324611686 1104 10-11 9.435276231723389 1104 12-13 9.486806072939853 1104 14-15 10.028965923847526 1104 16-17 10.802380609570509 1104 18-19 11.623705108047265 1104 20-21 11.8391728669896 1104 22-23 11.961157448581382 1104 24-25 11.752395860530534 1104 26-27 11.773888153745435 1104 28-29 11.41023477535428 1104 30-31 11.505295888793103 1104 32-33 11.57605417550208 1104 34-35 11.180611308961598 1104 36-37 11.254248819861346 1104 38-39 10.944297448472575 1104 40-41 10.586654755223517 1104 42-43 10.395140799043329 1104 44-45 10.504571621937512 1104 46-47 10.356194517098576 1104 48-49 10.060804213889416 1104 50-51 11.380511241793554 1104 52-53 11.05731581132832 1104 54-55 10.78068290866981 1104 56-57 10.501745537456706 1104 58-59 10.446411334852602 1104 60-61 10.286892771117028 1104 62-63 10.09171948288636 1104 64-65 9.870983447332582 1104 66-67 9.765413032817735 1104 68-69 9.637336998658375 1104 70-71 9.099633552285319 1104 72-73 8.815613293631657 1104 74-75 8.765964222100159 1104 76-77 8.341956726605572 1104 78-79 8.100131203332618 1104 80-81 8.013550886944563 1104 82-83 7.458283767077228 1104 84-85 6.9201729370148275 1104 86-87 6.514786657822301 1104 88-89 6.245241216246042 1104 90-91 5.846571417998806 1104 92-93 5.5136478120794745 1104 94-95 5.162005968116594 1104 96-97 4.57535498184563 1104 98-99 4.165703800202547 1104 100-101 3.6628392460876285 1105 1 8.080132707182788 1105 2 8.967939450929908 1105 3 8.532963598636346 1105 4 10.53195358893553 1105 5 10.26185635600822 1105 6 10.353774035989709 1105 7 9.892605156698242 1105 8 9.588562048957119 1105 9 9.497607362564576 1105 10-11 8.96648966500307 1105 12-13 8.864942864528668 1105 14-15 9.64163963933878 1105 16-17 10.181745585036463 1105 18-19 10.467459215910875 1105 20-21 10.903176479754634 1105 22-23 11.097351040206405 1105 24-25 11.249549447759467 1105 26-27 11.222338640655227 1105 28-29 10.875322513933607 1105 30-31 10.526753348026936 1105 32-33 10.477811586896134 1105 34-35 10.214637753367253 1105 36-37 10.425048670739162 1105 38-39 10.241298681101028 1105 40-41 9.954120933364191 1105 42-43 9.695509132990821 1105 44-45 9.36819512936884 1105 46-47 9.390547031595968 1105 48-49 9.295677174503489 1105 50-51 10.621768361192585 1105 52-53 9.75720862975913 1105 54-55 9.80168203807209 1105 56-57 9.747876468673024 1105 58-59 9.668755580826623 1105 60-61 9.259986264476563 1105 62-63 9.034830285840359 1105 64-65 8.804755662120249 1105 66-67 8.863331571592736 1105 68-69 8.468029985821369 1105 70-71 8.27510101369564 1105 72-73 7.864677481896258 1105 74-75 7.688455974048768 1105 76-77 7.539546717102263 1105 78-79 7.248148169213378 1105 80-81 6.901783595128398 1105 82-83 6.785291798556658 1105 84-85 6.516642140698055 1105 86-87 6.389132228009929 1105 88-89 5.971654940874261 1105 90-91 5.532569690810515 1105 92-93 5.375258466342277 1105 94-95 4.944257007338743 1105 96-97 4.726603422204301 1105 98-99 4.260377800146518 1105 100-101 3.72921060450021 1106 1 7.640974470237101 1106 2 8.758640166794379 1106 3 8.498688791707973 1106 4 10.145295899220383 1106 5 10.793165449992003 1106 6 10.210118709836355 1106 7 9.283598018599378 1106 8 8.935972048301235 1106 9 8.60124927070413 1106 10-11 8.398508815306162 1106 12-13 8.350377247043209 1106 14-15 8.96689826009656 1106 16-17 9.865890044416158 1106 18-19 10.795425541279535 1106 20-21 10.746269679634835 1106 22-23 10.92011274876683 1106 24-25 10.612431254655444 1106 26-27 10.802120309498964 1106 28-29 10.929061145564877 1106 30-31 10.266516383374473 1106 32-33 10.520126534073572 1106 34-35 10.278123212839397 1106 36-37 10.497278163027755 1106 38-39 9.966610088862456 1106 40-41 9.98827266281025 1106 42-43 9.732870952340736 1106 44-45 9.43189809816366 1106 46-47 9.076092559201278 1106 48-49 9.104071809510597 1106 50-51 10.703346210564709 1106 52-53 10.242091830611308 1106 54-55 9.389620207738059 1106 56-57 9.352276597321591 1106 58-59 9.19322508329331 1106 60-61 9.003025175926364 1106 62-63 9.041380260039526 1106 64-65 8.753199433293327 1106 66-67 8.648367562372394 1106 68-69 8.196496365320412 1106 70-71 7.843323474565693 1106 72-73 7.804097085630229 1106 74-75 7.393345804885385 1106 76-77 7.170167881055695 1106 78-79 6.71193415110301 1106 80-81 6.338668553440099 1106 82-83 6.092036642143553 1106 84-85 5.891792184595035 1106 86-87 5.642698116542428 1106 88-89 5.357264200094006 1106 90-91 5.09742875820324 1106 92-93 4.968051775984678 1106 94-95 4.727972730358697 1106 96-97 4.179314998915748 1106 98-99 3.699390240652484 1106 100-101 3.2746580020140037 1107 1 7.276088152436415 1107 2 8.084975567897818 1107 3 8.18368980175304 1107 4 9.602910596445259 1107 5 10.379415130742057 1107 6 9.593652943192229 1107 7 9.6733633202509 1107 8 8.279686732417442 1107 9 5.814702805377095 1107 10-11 7.759268092945302 1107 12-13 7.945392073654798 1107 14-15 8.098740286126105 1107 16-17 8.944374132122334 1107 18-19 10.196963346516018 1107 20-21 10.345519590019066 1107 22-23 9.81709439410302 1107 24-25 9.970262464105055 1107 26-27 10.081787511270374 1107 28-29 10.173611251835432 1107 30-31 10.072022012200907 1107 32-33 10.126019433171198 1107 34-35 10.352111637098702 1107 36-37 10.458657320133526 1107 38-39 10.048057905126427 1107 40-41 9.801784143921477 1107 42-43 9.658385939191557 1107 44-45 9.077035730583493 1107 46-47 9.221301645170922 1107 48-49 9.403560465064242 1107 50-51 10.405494345992297 1107 52-53 9.515425565040776 1107 54-55 9.702298750303456 1107 56-57 9.29113088613498 1107 58-59 9.258266673110889 1107 60-61 9.112379720604554 1107 62-63 8.955208607097118 1107 64-65 9.16496950639509 1107 66-67 9.07120204500553 1107 68-69 8.630512398806365 1107 70-71 8.43370537519559 1107 72-73 8.105000136311649 1107 74-75 8.039881695618298 1107 76-77 7.9062233157290205 1107 78-79 7.642615043666753 1107 80-81 7.371536787914524 1107 82-83 6.976552963369494 1107 84-85 7.010339268300742 1107 86-87 6.609214425283918 1107 88-89 6.311473232539898 1107 90-91 6.003242420892281 1107 92-93 5.373596491762244 1107 94-95 5.044745508203103 1107 96-97 4.580821194589303 1107 98-99 4.249244165955899 1107 100-101 3.8033147270746888 1108 1 6.62699790813604 1108 2 7.675719294577306 1108 3 7.3904876328159474 1108 4 8.657977959872959 1108 5 10.294952430539738 1108 6 8.931892629554103 1108 7 8.470971707317844 1108 8 5.450997793560827 1108 9 6.697109235022424 1108 10-11 5.6990312221839154 1108 12-13 4.734280695344488 1108 14-15 6.595011196530169 1108 16-17 7.819792579354271 1108 18-19 7.372503521436126 1108 20-21 7.257713962113058 1108 22-23 8.334797588465015 1108 24-25 8.016231871952069 1108 26-27 6.513934688309359 1108 28-29 8.45869392356288 1108 30-31 8.797683848378451 1108 32-33 9.387683940395942 1108 34-35 8.833778878612705 1108 36-37 8.563386046220451 1108 38-39 8.151841388212361 1108 40-41 8.534649795987022 1108 42-43 7.8819524169646265 1108 44-45 7.405296988335294 1108 46-47 8.079721927485625 1108 48-49 8.641527200801164 1108 50-51 10.09102577065532 1108 52-53 9.519744426747387 1108 54-55 8.959264411988169 1108 56-57 8.99626329854339 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2313 60-61 -14.387254113155972 2313 62-63 -13.945743425125508 2313 64-65 -13.575418629818751 2313 66-67 -13.188592901900366 2313 68-69 -12.73803392122293 2313 70-71 -12.361790780058897 2313 72-73 -11.975373353252614 2313 74-75 -11.517789487098653 2313 76-77 -11.08627027636179 2313 78-79 -10.699201140881032 2313 80-81 -10.267606383085111 2313 82-83 -9.816196945088945 2313 84-85 -9.387134184646895 2313 86-87 -8.917149543958116 2313 88-89 -8.486769169510636 2313 90-91 -8.028614041941847 2313 92-93 -7.514356638959102 2313 94-95 -6.987660203990233 2313 96-97 -6.398124246641016 2313 98-99 -5.787189374871637 2313 100-101 -5.004631080321868 2314 1 -17.839620379236965 2314 2 -16.71143600295535 2314 3 -17.298917301872006 2314 4 -18.80646325928524 2314 5 -18.13175293956186 2314 6 -18.998804032274634 2314 7 -19.118288089489123 2314 8 -19.474981887135836 2314 9 -20.58881239052184 2314 10-11 -20.841244539789958 2314 12-13 -20.6875262712738 2314 14-15 -20.61235455092992 2314 16-17 -19.589496245028894 2314 18-19 -18.631487770291013 2314 20-21 -18.294172830339775 2314 22-23 -17.951305459430486 2314 24-25 -17.762070014840386 2314 26-27 -17.45830769195189 2314 28-29 -17.23887501693059 2314 30-31 -17.131017167586716 2314 32-33 -16.968085290373292 2314 34-35 -16.608891658397454 2314 36-37 -16.1960479160437 2314 38-39 -16.030850919479946 2314 40-41 -15.82710637237292 2314 42-43 -15.63564555110504 2314 44-45 -15.255443214857738 2314 46-47 -14.92644643245632 2314 48-49 -14.622986587297529 2314 50-51 -12.741521399156001 2314 52-53 -14.468463120422424 2314 54-55 -15.049806705573513 2314 56-57 -14.97507196996169 2314 58-59 -14.677104985622176 2314 60-61 -14.387254113155972 2314 62-63 -13.945743425125508 2314 64-65 -13.575418629818751 2314 66-67 -13.188592901900366 2314 68-69 -12.73803392122293 2314 70-71 -12.361790780058897 2314 72-73 -11.975373353252614 2314 74-75 -11.517789487098653 2314 76-77 -11.08627027636179 2314 78-79 -10.699201140881032 2314 80-81 -10.267606383085111 2314 82-83 -9.816196945088945 2314 84-85 -9.387134184646895 2314 86-87 -8.917149543958116 2314 88-89 -8.486769169510636 2314 90-91 -8.028614041941847 2314 92-93 -7.514356638959102 2314 94-95 -6.987660203990233 2314 96-97 -6.398124246641016 2314 98-99 -5.787189374871637 2314 100-101 -5.004631080321868 2315 1 -17.839620379236965 2315 2 -16.71143600295535 2315 3 -17.298917301872006 2315 4 -18.80646325928524 2315 5 -18.13175293956186 2315 6 -18.998804032274634 2315 7 -19.118288089489123 2315 8 -19.474981887135836 2315 9 -20.58881239052184 2315 10-11 -20.841244539789958 2315 12-13 -20.6875262712738 2315 14-15 -20.61235455092992 2315 16-17 -19.589496245028894 2315 18-19 -18.631487770291013 2315 20-21 -18.294172830339775 2315 22-23 -17.951305459430486 2315 24-25 -17.762070014840386 2315 26-27 -17.45830769195189 2315 28-29 -17.23887501693059 2315 30-31 -17.131017167586716 2315 32-33 -16.968085290373292 2315 34-35 -16.608891658397454 2315 36-37 -16.1960479160437 2315 38-39 -16.030850919479946 2315 40-41 -15.82710637237292 2315 42-43 -15.63564555110504 2315 44-45 -15.255443214857738 2315 46-47 -14.92644643245632 2315 48-49 -14.622986587297529 2315 50-51 -12.741521399156001 2315 52-53 -14.468463120422424 2315 54-55 -15.049806705573513 2315 56-57 -14.97507196996169 2315 58-59 -14.677104985622176 2315 60-61 -14.387254113155972 2315 62-63 -13.945743425125508 2315 64-65 -13.575418629818751 2315 66-67 -13.188592901900366 2315 68-69 -12.73803392122293 2315 70-71 -12.361790780058897 2315 72-73 -11.975373353252614 2315 74-75 -11.517789487098653 2315 76-77 -11.08627027636179 2315 78-79 -10.699201140881032 2315 80-81 -10.267606383085111 2315 82-83 -9.816196945088945 2315 84-85 -9.387134184646895 2315 86-87 -8.917149543958116 2315 88-89 -8.486769169510636 2315 90-91 -8.028614041941847 2315 92-93 -7.514356638959102 2315 94-95 -6.987660203990233 2315 96-97 -6.398124246641016 2315 98-99 -5.787189374871637 2315 100-101 -5.004631080321868 2316 1 -17.839620379236965 2316 2 -16.71143600295535 2316 3 -17.298917301872006 2316 4 -18.80646325928524 2316 5 -18.13175293956186 2316 6 -18.998804032274634 2316 7 -19.118288089489123 2316 8 -19.474981887135836 2316 9 -20.58881239052184 2316 10-11 -20.841244539789958 2316 12-13 -20.6875262712738 2316 14-15 -20.61235455092992 2316 16-17 -19.589496245028894 2316 18-19 -18.631487770291013 2316 20-21 -18.294172830339775 2316 22-23 -17.951305459430486 2316 24-25 -17.762070014840386 2316 26-27 -17.45830769195189 2316 28-29 -17.23887501693059 2316 30-31 -17.131017167586716 2316 32-33 -16.968085290373292 2316 34-35 -16.608891658397454 2316 36-37 -16.1960479160437 2316 38-39 -16.030850919479946 2316 40-41 -15.82710637237292 2316 42-43 -15.63564555110504 2316 44-45 -15.255443214857738 2316 46-47 -14.92644643245632 2316 48-49 -14.622986587297529 2316 50-51 -12.741521399156001 2316 52-53 -14.468463120422424 2316 54-55 -15.049806705573513 2316 56-57 -14.97507196996169 2316 58-59 -14.677104985622176 2316 60-61 -14.387254113155972 2316 62-63 -13.945743425125508 2316 64-65 -13.575418629818751 2316 66-67 -13.188592901900366 2316 68-69 -12.73803392122293 2316 70-71 -12.361790780058897 2316 72-73 -11.975373353252614 2316 74-75 -11.517789487098653 2316 76-77 -11.08627027636179 2316 78-79 -10.699201140881032 2316 80-81 -10.267606383085111 2316 82-83 -9.816196945088945 2316 84-85 -9.387134184646895 2316 86-87 -8.917149543958116 2316 88-89 -8.486769169510636 2316 90-91 -8.028614041941847 2316 92-93 -7.514356638959102 2316 94-95 -6.987660203990233 2316 96-97 -6.398124246641016 2316 98-99 -5.787189374871637 2316 100-101 -5.004631080321868 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 731030.0 3 33280.0 4 94561.0 5 57005.0 6 38326.0 7 37030.0 8 35724.0 9 36407.0 10 38764.0 11 39744.0 12 39930.0 13 40383.0 14 40675.0 15 40827.0 16 42459.0 17 42151.0 18 42336.0 19 42727.0 20 44930.0 21 46999.0 22 49209.0 23 51995.0 24 54164.0 25 57382.0 26 60542.0 27 64011.0 28 67473.0 29 72440.0 30 76512.0 31 81649.0 32 85356.0 33 87074.0 34 85618.0 35 72207.0 36 45018.0 37 15007.0 38 1360.0 39 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.04827538233745 20.661476085897984 14.115282567002216 23.17496596476235 2 14.728910841654388 21.30332345131124 41.73332524853914 22.234440458495232 3 18.373453299134844 26.758860003584516 30.51205858122467 24.355628116055968 4 12.09745892527332 17.864880123946325 40.42878994774297 29.608871003037386 5 12.491215057677296 38.15749713273218 36.088943677931994 13.26234413165853 6 28.822117071197745 39.31672699207997 18.705039682703458 13.15611625401883 7 25.512277209696865 33.37632293463021 23.641771856423546 17.469627999249376 8 24.1251022827283 36.82255504428951 21.22838779234505 17.823954880637142 9 25.15582213537445 17.20974759136176 21.748765559211535 35.885664714052254 10-11 23.355366223380734 27.36946123973343 29.90791710002283 19.367255436863008 12-13 24.31302961569559 24.97102399574637 29.74669961824429 20.969246770313745 14-15 21.611721964270203 26.82352641116143 27.38189621963476 24.182855404933605 16-17 21.11587179099689 29.718106275846868 28.084164960068357 21.081856973087877 18-19 20.79367869710759 28.747541827323758 30.132537469593846 20.3262420059748 20-21 21.603460608331346 27.984549882489183 30.15906536326169 20.252924145917778 22-23 21.117877485256475 27.977926228071624 29.908839504380325 20.995356782291573 24-25 21.01421606023417 28.072148329961195 30.16771368867956 20.74592192112508 26-27 20.968968934269043 28.22938414244592 29.89158179328096 20.91006513000408 28-29 20.99308491799635 28.47366546083756 29.9514812172123 20.58176840395379 30-31 21.33424412867364 28.115362318222797 29.915017892652614 20.63537566045095 32-33 21.042589076149344 28.230032667451233 29.857046728932673 20.87033152746675 34-35 21.23594156719289 28.311969285122434 29.85096403790366 20.601125109781012 36-37 21.24921596125616 28.197138728408444 29.709610442369787 20.84403486796561 38-39 21.232877809641295 28.325750172223202 29.743961507446986 20.69741051068852 40-41 21.495529612566422 28.31812090381166 29.570793571340285 20.61555591228163 42-43 21.30365280338119 28.293570146661068 29.370738037189557 21.032039012768184 44-45 21.409351887645972 28.43734349516167 29.347251787788874 20.80605282940348 46-47 21.446209814695656 28.387992402090013 28.991021863449802 21.174775919764535 48-49 21.382784066413336 28.503769839280523 29.140506722545844 20.972939371760294 50-51 21.544560590891408 28.715275139826797 28.77659426341755 20.96357000586425 52-53 21.090262173027455 28.886632329054024 28.87475729310663 21.148348204811892 54-55 21.124235592422323 28.81004693458123 28.945181083534294 21.120536389462153 56-57 21.08407907949477 28.962170248757996 28.99399496134591 20.959755710401325 58-59 21.084790564488614 28.901610975354362 29.025419764342914 20.988178695814106 60-61 21.102260474317262 28.989657822454657 28.986397314768137 20.921684388459944 62-63 21.07683618174451 28.968079865663654 28.971867353532254 20.983216599059585 64-65 20.888203335936574 28.79566765111007 29.046965031812384 21.269163981140977 66-67 20.88448931032925 28.926866488296326 28.73265030545502 21.455993895919402 68-69 21.05570178594905 28.851546243373544 28.83422915165831 21.2585228190191 70-71 21.15584276991756 28.601103267514944 28.858519056348225 21.38453490621927 72-73 21.037596309135996 28.7511874922379 28.821099269995603 21.390116928630505 74-75 21.01553468277585 29.068717311476544 28.46217027751222 21.45357772823538 76-77 21.065949593937276 28.84862667590719 28.563295334515864 21.522128395639665 78-79 20.930125040636923 28.913993069034948 28.57757194111297 21.57830994921516 80-81 20.99054118747231 28.99764552348241 28.238843135636145 21.772970153409133 82-83 21.170447945345238 28.76027853794401 28.384920608062803 21.68435290864795 84-85 21.268785641451487 28.772047336838675 28.20159122029482 21.757575801415012 86-87 21.078442462347148 28.868983215561606 28.29991167120442 21.75266265088683 88-89 21.200277869919177 28.74322982832301 28.270308213178662 21.78618408857915 90-91 21.133838532201203 28.846163773617743 28.2975853552473 21.722412338933754 92-93 21.171468823191304 28.82829772641432 28.193533817255524 21.806699633138848 94-95 21.109834371345425 28.68429538858433 28.183363897382502 22.022506342687745 96-97 21.060970618586204 28.727434443407486 28.17722294426256 22.034371993743754 98-99 20.91484638611929 28.76803184339985 27.92368918643289 22.393432584047968 100-101 21.23270744991266 28.870972597006748 27.907920660367374 21.98839929271322 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 157161.0 1 142743.0 2 94682.0 3 47688.5 4 29552.5 5 23254.0 6 21498.0 7 21564.5 8 22106.5 9 21992.5 10 20310.5 11 17312.0 12 13735.0 13 10311.5 14 7726.0 15 6144.0 16 5610.5 17 5874.0 18 6787.5 19 8087.5 20 9699.5 21 11462.5 22 13260.0 23 15139.5 24 17383.5 25 19716.0 26 22717.5 27 26413.0 28 30330.0 29 34746.0 30 39184.5 31 44123.0 32 49067.0 33 53637.5 34 58165.0 35 62210.0 36 66286.5 37 70451.5 38 73948.5 39 75938.5 40 77067.5 41 77696.5 42 77605.0 43 77692.0 44 77635.5 45 77136.0 46 75920.5 47 73970.5 48 71337.5 49 68014.0 50 63625.0 51 58534.5 52 53409.5 53 48059.5 54 42384.5 55 36458.5 56 30672.5 57 25459.0 58 20604.5 59 16229.0 60 12650.0 61 9512.0 62 6838.5 63 4802.0 64 3314.0 65 2190.0 66 1398.5 67 891.0 68 584.5 69 373.5 70 240.0 71 153.0 72 116.0 73 90.0 74 58.5 75 38.0 76 26.0 77 21.5 78 17.0 79 11.0 80 9.0 81 8.0 82 6.0 83 3.0 84 1.5 85 1.0 86 0.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.36184897782208 2 29.38350689810007 3 23.16109814111595 4 29.488008369375862 5 32.59643530012637 6 25.225860507316263 7 24.146089122125918 8 20.139042119995 9 11.954520836258654 10-11 15.601637614048947 12-13 15.695627217136234 14-15 19.83925135439153 16-17 23.16580437201135 18-19 27.146079863966783 20-21 29.216069232514037 22-23 28.796636047877026 24-25 27.311395868083576 26-27 28.780434269384656 28-29 25.716928698287393 30-31 27.603201471430094 32-33 26.59188260037002 34-35 24.64936651046095 36-37 27.643455175851017 38-39 27.764370591766102 40-41 24.783976286768393 42-43 25.650462830805598 44-45 24.36076268714983 46-47 27.640581288951775 48-49 23.050038807117055 50-51 28.660425381551885 52-53 22.19921398228914 54-55 23.35345568504977 56-57 25.454054842248684 58-59 24.75475522198751 60-61 24.280428868791827 62-63 24.1215550004089 64-65 22.64831185183242 66-67 23.15303582753284 68-69 24.15000455192824 70-71 22.64360562093702 72-73 22.503016616852626 74-75 19.782834447141312 76-77 22.045972160715472 78-79 21.150746747685844 80-81 22.200737720980687 82-83 23.055844444410155 84-85 20.00597537021064 86-87 21.563641357431294 88-89 20.396978291159847 90-91 21.53769922401196 92-93 21.510349078890318 94-95 20.570240881870518 96-97 19.46043834297468 98-99 18.86407787963467 100-101 20.545629608827344 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2592308.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.63993189277227 #Duplication Level Percentage of deduplicated Percentage of total 1 91.4558087287929 74.6644599581706 2 5.9038090579389655 9.639731387961383 3 1.3403530495231613 3.2827899502602147 4 0.4944087323535908 1.6145398094615608 5 0.24678901568653713 1.0073919216266598 6 0.14664872027911513 0.7183434921449503 7 0.09571109975801216 0.5469693365938512 8 0.06464341937160821 0.42219874838512084 9 0.04888418112331637 0.3591811095787343 >10 0.19507839334194474 2.5682497661921837 >50 0.005702330545139672 0.32227374514782525 >100 0.0019758915521901565 0.255391567525421 >500 4.6844933389711666E-5 0.024667490755900967 >1k 9.368986677923746E-5 0.11056390042661736 >5k 0.0 0.0 >10k+ 4.684493338961873E-5 4.463247815768922 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 116704 4.501934183746684 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.857566307707263E-5 0.0 4 0.0 0.0 0.0 3.857566307707263E-5 0.0 5 0.0 3.857566307707263E-5 0.0 3.857566307707263E-5 0.0 6 0.0 3.857566307707263E-5 0.0 3.857566307707263E-5 3.857566307707263E-5 7 0.0 3.857566307707263E-5 3.857566307707263E-5 3.857566307707263E-5 3.857566307707263E-5 8 0.0 3.857566307707263E-5 3.857566307707263E-5 3.857566307707263E-5 3.857566307707263E-5 9 0.0 3.857566307707263E-5 3.857566307707263E-5 7.715132615414526E-5 3.857566307707263E-5 10-11 0.0 3.857566307707263E-5 3.857566307707263E-5 1.350148207697542E-4 3.857566307707263E-5 12-13 0.0 3.857566307707263E-5 3.857566307707263E-5 1.5430265230829051E-4 3.857566307707263E-5 14-15 0.0 3.857566307707263E-5 3.857566307707263E-5 1.5430265230829051E-4 3.857566307707263E-5 16-17 0.0 3.857566307707263E-5 3.857566307707263E-5 1.5430265230829051E-4 3.857566307707263E-5 18-19 0.0 3.857566307707263E-5 3.857566307707263E-5 1.9287831538536315E-4 3.857566307707263E-5 20-21 0.0 3.857566307707263E-5 3.857566307707263E-5 2.3145397846243578E-4 3.857566307707263E-5 22-23 0.0 3.857566307707263E-5 3.857566307707263E-5 3.4718096769365366E-4 3.857566307707263E-5 24-25 0.0 3.857566307707263E-5 3.857566307707263E-5 4.2433229384779893E-4 3.857566307707263E-5 26-27 0.0 3.857566307707263E-5 3.857566307707263E-5 4.629079569248716E-4 3.857566307707263E-5 28-29 0.0 3.857566307707263E-5 3.857566307707263E-5 7.715132615414526E-4 3.857566307707263E-5 30-31 0.0 3.857566307707263E-5 3.857566307707263E-5 0.0016394656807755868 3.857566307707263E-5 32-33 0.0 3.857566307707263E-5 3.857566307707263E-5 0.0034139461823209276 3.857566307707263E-5 34-35 0.0 3.857566307707263E-5 3.857566307707263E-5 0.006210681755408693 3.857566307707263E-5 36-37 0.0 5.786349461560894E-5 3.857566307707263E-5 0.010531156020040828 3.857566307707263E-5 38-39 3.857566307707263E-5 7.715132615414526E-5 3.857566307707263E-5 0.01685756476468074 3.857566307707263E-5 40-41 3.857566307707263E-5 7.715132615414526E-5 3.857566307707263E-5 0.023685457129322594 3.857566307707263E-5 42-43 3.857566307707263E-5 7.715132615414526E-5 3.857566307707263E-5 0.030706227809349815 3.857566307707263E-5 44-45 3.857566307707263E-5 7.715132615414526E-5 3.857566307707263E-5 0.04029228008400236 3.857566307707263E-5 46-47 3.857566307707263E-5 7.715132615414526E-5 3.857566307707263E-5 0.05165281286020025 3.857566307707263E-5 48-49 3.857566307707263E-5 7.715132615414526E-5 3.857566307707263E-5 0.06538574891563811 3.857566307707263E-5 50-51 3.857566307707263E-5 7.715132615414526E-5 3.857566307707263E-5 0.08052669667338912 3.857566307707263E-5 52-53 3.857566307707263E-5 7.715132615414526E-5 3.857566307707263E-5 0.09802075987884155 3.857566307707263E-5 54-55 3.857566307707263E-5 7.715132615414526E-5 3.857566307707263E-5 0.11921808673969297 3.857566307707263E-5 56-57 3.857566307707263E-5 7.715132615414526E-5 3.857566307707263E-5 0.14299998302670824 3.857566307707263E-5 58-59 3.857566307707263E-5 7.715132615414526E-5 3.857566307707263E-5 0.1695207513921957 3.857566307707263E-5 60-61 3.857566307707263E-5 7.715132615414526E-5 3.857566307707263E-5 0.20026555486462255 3.857566307707263E-5 62-63 3.857566307707263E-5 7.715132615414526E-5 3.857566307707263E-5 0.23318988330090407 3.857566307707263E-5 64-65 3.857566307707263E-5 7.715132615414526E-5 3.857566307707263E-5 0.26590204559026165 3.857566307707263E-5 66-67 3.857566307707263E-5 7.715132615414526E-5 3.857566307707263E-5 0.3017581244204006 3.857566307707263E-5 68-69 3.857566307707263E-5 7.715132615414526E-5 3.857566307707263E-5 0.34328482572286934 3.857566307707263E-5 70-71 3.857566307707263E-5 7.715132615414526E-5 3.857566307707263E-5 0.3904242860030521 3.857566307707263E-5 72-73 3.857566307707263E-5 7.715132615414526E-5 3.857566307707263E-5 0.4407076628240163 3.857566307707263E-5 74-75 1.1572698923121789E-4 7.715132615414526E-5 3.857566307707263E-5 0.492341187852678 3.857566307707263E-5 76-77 1.1572698923121789E-4 7.715132615414526E-5 3.857566307707263E-5 0.5519020116436781 3.857566307707263E-5 78-79 1.1572698923121789E-4 7.715132615414526E-5 3.857566307707263E-5 0.6173841997170089 3.857566307707263E-5 80-81 1.1572698923121789E-4 7.715132615414526E-5 3.857566307707263E-5 0.6908322622157552 3.857566307707263E-5 82-83 1.5430265230829051E-4 7.715132615414526E-5 3.857566307707263E-5 0.7689094042837503 3.857566307707263E-5 84-85 1.5430265230829051E-4 7.715132615414526E-5 3.857566307707263E-5 0.8491082078209842 3.857566307707263E-5 86-87 1.5430265230829051E-4 7.715132615414526E-5 3.857566307707263E-5 0.9313900971643803 3.857566307707263E-5 88-89 1.5430265230829051E-4 7.715132615414526E-5 3.857566307707263E-5 1.0215221339439604 3.857566307707263E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1795 0.0 57.663773 1 GTATCAA 3420 0.0 43.59965 1 TCAACGC 4170 0.0 35.750694 4 ATCAACG 4225 0.0 35.40965 3 CAACGCA 4285 0.0 34.669662 5 TATCAAC 4370 0.0 33.877953 2 AACGCAG 4675 0.0 33.13451 6 ACGCAGA 5355 0.0 27.841963 7 CGCAGAG 5350 0.0 27.60453 8 GTACATG 3765 0.0 24.769802 1 GCAGAGT 6030 0.0 23.058395 9 TACATGG 3765 0.0 22.722298 2 ACATGGG 3760 0.0 21.702963 3 GTGTAGC 905 0.0 21.515486 1 CATGGGG 2360 0.0 20.48746 4 GAGTACT 3845 0.0 20.23535 12-13 CAGAGTA 5705 0.0 19.508778 10-11 AGAGTAC 5110 0.0 19.360308 10-11 GTATAAC 705 0.0 18.170506 1 AGTACTT 4330 0.0 17.192375 12-13 >>END_MODULE