FastQCFastQC Report
Sat 18 Jun 2016
SRR1931050_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931050_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2592308
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62560.24132934821016636No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54130.20881006423619417No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30840.118967344929692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30750.054.7143171
GTATCAA60700.042.437851
ATCAACG76250.033.344423
TCAACGC76400.033.2789574
CAACGCA77900.032.5212255
TATCAAC80650.031.5258662
AACGCAG81600.031.3954496
CGCAGAG94650.026.8662628
ACGCAGA94850.026.7595967
GCAGAGT109500.022.399569
GTACATG61850.021.5159781
TACATGG61150.021.2169932
ACATGGG58350.020.9315573
AGAGTAC102700.020.88002810-11
GAGTACT83450.019.27242912-13
GTGGTAT17350.019.1752641
CAGAGTA107600.018.67257510-11
AGTACTT89800.018.57000712-13
GTACTTT89150.017.85394714-15
TGGTATC17750.017.4033412
Produced by FastQC (version 0.11.5)