##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931050_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2592308 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.355452361370638 31.0 31.0 34.0 30.0 34.0 2 31.508981957390866 31.0 31.0 34.0 30.0 34.0 3 31.57454669738318 31.0 31.0 34.0 30.0 34.0 4 35.349017940769386 37.0 35.0 37.0 33.0 37.0 5 35.222769825190525 37.0 35.0 37.0 33.0 37.0 6 35.252483886945534 37.0 35.0 37.0 32.0 37.0 7 35.13996292107265 37.0 35.0 37.0 32.0 37.0 8 35.14256022046763 37.0 35.0 37.0 32.0 37.0 9 36.65104802361448 39.0 35.0 39.0 32.0 39.0 10-11 36.594860062924624 38.5 35.0 39.0 32.0 39.0 12-13 36.48231267272253 38.0 35.0 39.0 32.0 39.0 14-15 37.55017054300646 39.0 36.0 41.0 32.0 41.0 16-17 37.508642105799154 39.0 36.0 41.0 32.0 41.0 18-19 37.5310227411249 39.0 36.0 41.0 32.0 41.0 20-21 37.51648511673767 39.0 36.0 41.0 32.0 41.0 22-23 37.416689104844025 39.0 36.0 41.0 32.0 41.0 24-25 37.293411701078725 39.0 36.0 41.0 31.5 41.0 26-27 37.04847244231781 39.0 36.0 40.0 31.0 41.0 28-29 36.859694334160906 39.0 36.0 40.0 30.5 41.0 30-31 36.63311400497163 38.5 35.5 40.0 30.0 41.0 32-33 36.46848156160456 38.0 35.0 40.0 30.0 41.0 34-35 36.54046471329796 38.0 35.0 40.0 30.0 41.0 36-37 36.609650357905004 39.0 35.0 40.0 30.0 41.0 38-39 36.587749989584566 39.0 35.0 40.0 30.0 41.0 40-41 36.478051412100726 39.0 35.0 40.0 30.0 41.0 42-43 36.34248206617424 39.0 35.0 40.0 30.0 41.0 44-45 36.174480038637384 38.5 35.0 40.0 30.0 41.0 46-47 35.94473341902274 38.0 35.0 40.0 29.0 41.0 48-49 35.73921193006387 38.0 35.0 40.0 28.0 41.0 50-51 35.47952191637722 38.0 34.5 40.0 27.5 41.0 52-53 35.2780014951927 38.0 34.0 40.0 27.0 41.0 54-55 35.13024609730017 38.0 34.0 40.0 26.5 41.0 56-57 34.966608906040484 38.0 34.0 40.0 26.0 41.0 58-59 34.764554019044034 38.0 33.5 40.0 26.0 41.0 60-61 34.46959504811928 38.0 33.0 40.0 26.0 41.0 62-63 34.22633633812032 37.0 33.0 40.0 25.0 41.0 64-65 33.92336925241908 37.0 32.5 40.0 25.0 41.0 66-67 33.61253523886822 36.5 32.0 39.0 23.5 41.0 68-69 33.26756812847856 36.0 32.0 39.0 23.0 40.0 70-71 32.894551881952296 36.0 31.5 39.0 22.0 40.0 72-73 32.46801132427166 35.0 31.0 38.0 22.0 40.0 74-75 31.95044184564489 35.0 31.0 37.5 21.0 39.5 76-77 30.565307440319593 33.5 29.5 36.0 20.0 39.0 78-79 31.296462071636547 34.0 30.0 36.0 20.5 39.0 80-81 31.25708499915905 34.0 31.0 36.0 20.5 38.5 82-83 30.97605319275333 34.0 30.5 36.0 20.0 37.0 84-85 30.629476127065146 34.0 30.0 35.0 19.5 37.0 86-87 30.270877534613945 34.0 30.0 35.0 18.0 36.5 88-89 29.90915296330529 34.0 30.0 35.0 16.0 36.0 90-91 29.56425085290791 34.0 29.0 35.0 9.5 36.0 92-93 29.243305965186234 34.0 29.0 35.0 6.0 35.5 94-95 28.859070179932324 34.0 29.0 35.0 2.0 35.0 96-97 28.4267907208557 34.0 29.0 35.0 2.0 35.0 98-99 27.926139563662957 33.5 29.0 35.0 2.0 35.0 100-101 26.785834090702185 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39492227044892303 1101 2 0.362248294103086 1101 3 0.3246617941252765 1101 4 0.1737038584905406 1101 5 0.2756564514255331 1101 6 0.2961771078275319 1101 7 0.36544927914086855 1101 8 0.4350452532644624 1101 9 0.505263763430122 1101 10-11 0.48029806725751456 1101 12-13 0.4561564190924301 1101 14-15 0.6312706526238898 1101 16-17 0.5994528966692485 1101 18-19 0.5912143079076984 1101 20-21 0.612569136253164 1101 22-23 0.5096678973816466 1101 24-25 0.5627892212639267 1101 26-27 0.4708892179165147 1101 28-29 0.7052537546420368 1101 30-31 0.6148130169647956 1101 32-33 0.6642827792831767 1101 34-35 0.530971285041602 1101 36-37 0.5812091478509345 1101 38-39 0.617091880318732 1101 40-41 0.6521325816229719 1101 42-43 0.39843679537708 1101 44-45 0.5237563068462308 1101 46-47 0.412847842026288 1101 48-49 0.4219471498284477 1101 50-51 0.5038194083311325 1101 52-53 0.44432351413079374 1101 54-55 0.49495351798730525 1101 56-57 0.4602619914387134 1101 58-59 0.34508742629045486 1101 60-61 0.40250226182982374 1101 62-63 0.1939835509192207 1101 64-65 0.35656930254715746 1101 66-67 0.3159702212411162 1101 68-69 0.420043575520495 1101 70-71 0.42163936144454084 1101 72-73 0.4470568339469736 1101 74-75 0.46372653135739483 1101 76-77 0.467023746402063 1101 78-79 0.3751431054155354 1101 80-81 0.3851545824055904 1101 82-83 0.4456894078404652 1101 84-85 0.37086447902539277 1101 86-87 0.6051955591533229 1101 88-89 0.5183838995789927 1101 90-91 0.4971759328431098 1101 92-93 0.416637245018908 1101 94-95 0.5383829592838687 1101 96-97 0.6512528989461366 1101 98-99 0.7036550463859577 1101 100-101 0.987340621954889 1102 1 0.2610176285406496 1102 2 0.41564311029731016 1102 3 0.32945764817835155 1102 4 0.05613936367938521 1102 5 0.29880573886327255 1102 6 0.2829752354220716 1102 7 0.35096760751816447 1102 8 0.2722041139165299 1102 9 0.4175239147987 1102 10-11 0.38920443177165254 1102 12-13 0.43721700142567954 1102 14-15 0.41415020235410793 1102 16-17 0.40440238330288025 1102 18-19 0.5102601095368797 1102 20-21 0.39060749234726444 1102 22-23 0.40498715217786696 1102 24-25 0.5078219865698514 1102 26-27 0.5580450211296011 1102 28-29 0.5489508367117537 1102 30-31 0.5363504076538916 1102 32-33 0.606185547354201 1102 34-35 0.5018578289990643 1102 36-37 0.6061111681171312 1102 38-39 0.5720596817576151 1102 40-41 0.49070584337941625 1102 42-43 0.4734951328044019 1102 44-45 0.5835353539334776 1102 46-47 0.43519197900671003 1102 48-49 0.4598919065293856 1102 50-51 0.3246505007854026 1102 52-53 0.2937244350612431 1102 54-55 0.4739026776986677 1102 56-57 0.43825536589385905 1102 58-59 0.5502680553115482 1102 60-61 0.34721116231808935 1102 62-63 0.20248024843768775 1102 64-65 0.21882362241017006 1102 66-67 0.1468140535216449 1102 68-69 0.30519214766665925 1102 70-71 0.3675027807232354 1102 72-73 0.3377549561425184 1102 74-75 0.33918525906307195 1102 76-77 0.29218014559203453 1102 78-79 0.5769634598457962 1102 80-81 0.5074984509279581 1102 82-83 0.4360785348118803 1102 84-85 0.4909165360255976 1102 86-87 0.5125510563838809 1102 88-89 0.5399702391205672 1102 90-91 0.46299905955023846 1102 92-93 0.5233996587431982 1102 94-95 0.574654998571809 1102 96-97 0.7230549892798841 1102 98-99 0.692656096056961 1102 100-101 0.8380270095504585 1103 1 0.2914000099266332 1103 2 0.367222839540446 1103 3 0.3646737616240081 1103 4 0.17158901953047945 1103 5 0.2127675704782206 1103 6 0.2868550406400132 1103 7 0.3095660514485701 1103 8 0.3231977248689688 1103 9 0.47997784446828007 1103 10-11 0.3833196834782555 1103 12-13 0.4426021635272619 1103 14-15 0.4812540540693888 1103 16-17 0.5090883132590065 1103 18-19 0.4131009639989429 1103 20-21 0.404149105991813 1103 22-23 0.465500236623015 1103 24-25 0.47734063056095266 1103 26-27 0.5667095424034869 1103 28-29 0.5672451174867277 1103 30-31 0.4818672342824044 1103 32-33 0.566151726311638 1103 34-35 0.5546556059347765 1103 36-37 0.6705163117811566 1103 38-39 0.6236518535247697 1103 40-41 0.5920841356995581 1103 42-43 0.5303678363574988 1103 44-45 0.5308092157724431 1103 46-47 0.5673560921281009 1103 48-49 0.44235643119669277 1103 50-51 0.4160380118847016 1103 52-53 0.5220881111861146 1103 54-55 0.41822049746682666 1103 56-57 0.5383158818061489 1103 58-59 0.5459009179027206 1103 60-61 0.4079627284736702 1103 62-63 0.36775372946001283 1103 64-65 0.33051695566808803 1103 66-67 0.2896476309923841 1103 68-69 0.23397377948482756 1103 70-71 0.4526633741185151 1103 72-73 0.3359837817002145 1103 74-75 0.4346929423216608 1103 76-77 0.48404952171257065 1103 78-79 0.5353950608404396 1103 80-81 0.5243995460585964 1103 82-83 0.516289454446369 1103 84-85 0.5705920500809398 1103 86-87 0.4725587104213851 1103 88-89 0.48365179345574916 1103 90-91 0.46106292740309485 1103 92-93 0.4395087683674923 1103 94-95 0.49005997445027916 1103 96-97 0.5909276381612827 1103 98-99 0.6177945191367122 1103 100-101 0.7397942250078451 1104 1 0.37210621664327803 1104 2 0.4431221269919696 1104 3 0.39784431561723466 1104 4 0.27536365991107914 1104 5 0.303748356294264 1104 6 0.37610210279713385 1104 7 0.3913483334252632 1104 8 0.45347676231571654 1104 9 0.5581841720606704 1104 10-11 0.5114585174264548 1104 12-13 0.5871264078289542 1104 14-15 0.7726688962618837 1104 16-17 0.668063534292962 1104 18-19 0.6310737399695157 1104 20-21 0.5739291430528723 1104 22-23 0.5428139108746848 1104 24-25 0.6707835710237475 1104 26-27 0.7343011662201704 1104 28-29 0.7497914606861471 1104 30-31 0.6819103419472796 1104 32-33 0.6949582582121465 1104 34-35 0.6524037469782229 1104 36-37 0.8366109035191016 1104 38-39 0.6997715986624726 1104 40-41 0.8736463394836917 1104 42-43 0.860859980558331 1104 44-45 0.9182481032433074 1104 46-47 0.7660786322504194 1104 48-49 0.7585763120525613 1104 50-51 0.6868929435589806 1104 52-53 0.5897911796310211 1104 54-55 0.7590622058965337 1104 56-57 0.9730355990581003 1104 58-59 0.9782658772041444 1104 60-61 0.9108216507342632 1104 62-63 0.7165681773164678 1104 64-65 0.7854960807075884 1104 66-67 0.803061746805966 1104 68-69 0.6761054991925235 1104 70-71 0.8933219557906042 1104 72-73 0.8680212661128692 1104 74-75 0.9069882209711722 1104 76-77 0.8969149740745941 1104 78-79 0.959802460460164 1104 80-81 0.9077490383242299 1104 82-83 0.8522671790138325 1104 84-85 1.0724316805722118 1104 86-87 1.0592968980497801 1104 88-89 1.013630314116508 1104 90-91 0.9257774694864089 1104 92-93 0.8420596542461674 1104 94-95 1.0151945400790616 1104 96-97 1.0373485494407326 1104 98-99 1.0791267188616764 1104 100-101 1.2964680842500051 1105 1 0.30139601147098105 1105 2 0.31797909252793843 1105 3 0.3359639969439918 1105 4 0.1516326530561969 1105 5 0.21609085676296758 1105 6 0.28637828052836767 1105 7 0.22769966701288524 1105 8 0.2502175159861224 1105 9 0.2427158702218577 1105 10-11 0.3199456060744197 1105 12-13 0.37011618908049826 1105 14-15 0.3985753199369668 1105 16-17 0.3804376585772502 1105 18-19 0.4579052006557802 1105 20-21 0.3920015610518348 1105 22-23 0.371460508540018 1105 24-25 0.4758184380313253 1105 26-27 0.3559482067266728 1105 28-29 0.2819864713423499 1105 30-31 0.23496872048057327 1105 32-33 0.3887808663849981 1105 34-35 0.5453889166478021 1105 36-37 0.524482900872826 1105 38-39 0.4458222581833482 1105 40-41 0.34231694323459294 1105 42-43 0.29459138607800384 1105 44-45 0.27332442600232554 1105 46-47 0.2581786044516292 1105 48-49 0.17071207250027243 1105 50-51 0.191301826026141 1105 52-53 0.10134545514934246 1105 54-55 0.18194575375462563 1105 56-57 0.4017967589787119 1105 58-59 0.3923088985230052 1105 60-61 0.2921527440024221 1105 62-63 0.15923681252524347 1105 64-65 0.15841467335620507 1105 66-67 0.17689241985988247 1105 68-69 0.007760669228446204 1105 70-71 0.14918636881595404 1105 72-73 0.07616967824176157 1105 74-75 0.25177831856863264 1105 76-77 0.36845246257257713 1105 78-79 0.3302724970295756 1105 80-81 0.2806658800801678 1105 82-83 0.36073920998470044 1105 84-85 0.367518110022548 1105 86-87 0.40264641501889287 1105 88-89 0.30437533781012505 1105 90-91 0.44862230914773704 1105 92-93 0.4351124622470479 1105 94-95 0.32509877433018985 1105 96-97 0.3155504473006552 1105 98-99 0.3654339030167968 1105 100-101 0.44590057952528994 1106 1 0.17482850218781465 1106 2 0.18760151530916502 1106 3 0.17721142970180281 1106 4 0.06948603952423582 1106 5 0.0056236206604509675 1106 6 0.16936774762459805 1106 7 0.2421919911661803 1106 8 0.23166936404489746 1106 9 0.33389751678125634 1106 10-11 0.19039164045077683 1106 12-13 0.35126024465541406 1106 14-15 0.31342111155631613 1106 16-17 0.3056792499947818 1106 18-19 0.3313584347685108 1106 20-21 0.28021757368910727 1106 22-23 0.25836652633745416 1106 24-25 0.29547574922888487 1106 26-27 0.2771886934102028 1106 28-29 0.23780046985515213 1106 30-31 0.3016962739531053 1106 32-33 0.2721757187038705 1106 34-35 0.26947033981070945 1106 36-37 0.2693737036857158 1106 38-39 0.3021851071959176 1106 40-41 0.33710205350648437 1106 42-43 0.3283894900130875 1106 44-45 0.3432271019399096 1106 46-47 0.2579853945355737 1106 48-49 0.2823611290115977 1106 50-51 0.2489929635781678 1106 52-53 0.17349691275813228 1106 54-55 0.2967993685975685 1106 56-57 0.2835386589789479 1106 58-59 0.14785765814696106 1106 60-61 0.2770201403624952 1106 62-63 0.0016148591374332 1106 64-65 0.01781435552786803 1106 66-67 0.03027217098654944 1106 68-69 -0.03693166867475384 1106 70-71 0.15788792705985344 1106 72-73 0.2049172196554494 1106 74-75 0.39539808461473314 1106 76-77 0.18229806690869665 1106 78-79 0.36225604771418674 1106 80-81 0.24709397793842314 1106 82-83 0.29437535738687615 1106 84-85 0.43264486600040186 1106 86-87 0.2561166468628926 1106 88-89 0.258464276009029 1106 90-91 0.27779550504520856 1106 92-93 0.2844152470775789 1106 94-95 0.41215307777485677 1106 96-97 0.47636115452797156 1106 98-99 0.5584857741501317 1106 100-101 0.6004076650742114 1107 1 0.1783363667931681 1107 2 0.1886458262054198 1107 3 0.1911646610956268 1107 4 0.004189581003885223 1107 5 0.07352255559083432 1107 6 0.12975126990475871 1107 7 0.18229837726742204 1107 8 0.13294140988885772 1107 9 0.2061090851640941 1107 10-11 0.2011425442120114 1107 12-13 0.2478379383712337 1107 14-15 0.3171904191222765 1107 16-17 0.28633359982259776 1107 18-19 0.34774493844140153 1107 20-21 0.27450301627678186 1107 22-23 0.2805173259996323 1107 24-25 0.3014879412232432 1107 26-27 0.3199686508754169 1107 28-29 0.24553810570156287 1107 30-31 0.29242497664484546 1107 32-33 0.3269047550225892 1107 34-35 0.308878831919003 1107 36-37 0.42308284019080844 1107 38-39 0.36638559045035635 1107 40-41 0.3766366518332731 1107 42-43 0.35498787471129845 1107 44-45 0.38712906607580777 1107 46-47 0.4286597022164216 1107 48-49 0.24031071494625422 1107 50-51 0.13213513210308747 1107 52-53 0.17247982858625477 1107 54-55 0.21563517511357588 1107 56-57 0.25549679304253203 1107 58-59 0.2284370083101237 1107 60-61 0.24913718552711828 1107 62-63 0.04742986589696585 1107 64-65 0.16245553187111028 1107 66-67 0.11084049764667725 1107 68-69 0.10241291367857741 1107 70-71 0.07140356522704394 1107 72-73 -0.025137577779709375 1107 74-75 0.05574841685381671 1107 76-77 0.10276668000724598 1107 78-79 0.21400142020034352 1107 80-81 0.27277229857281426 1107 82-83 0.32225492548337087 1107 84-85 0.22712353864313783 1107 86-87 0.16123290696815573 1107 88-89 0.22763499115679764 1107 90-91 0.2209643663850791 1107 92-93 0.258664410387059 1107 94-95 0.3361813526617077 1107 96-97 0.4338016671235181 1107 98-99 0.45122516379603184 1107 100-101 0.7051525260963949 1108 1 0.04693383849234323 1108 2 0.12544136142194873 1108 3 0.05120811211411436 1108 4 0.046050114713764856 1108 5 -0.010265739737917556 1108 6 0.06501929668325346 1108 7 0.09791140810882837 1108 8 0.06235170686615277 1108 9 0.09730895187745858 1108 10-11 0.016503350819370155 1108 12-13 0.09865130306696557 1108 14-15 0.1642332414402432 1108 16-17 0.1031374813973116 1108 18-19 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-0.36119932117854603 2316 66-67 -0.8522082598871066 2316 68-69 -0.8429747918557027 2316 70-71 -0.8274601142367715 2316 72-73 -1.551677452148887 2316 74-75 -2.6323407767049183 2316 76-77 -1.6978372816066205 2316 78-79 -1.4576285477237754 2316 80-81 -1.1120057667579069 2316 82-83 -0.8874025407100561 2316 84-85 -0.872312926906222 2316 86-87 -0.8610662802703999 2316 88-89 -0.7124306316466047 2316 90-91 -0.8054894606901648 2316 92-93 -0.7426240835921121 2316 94-95 -1.057685291933371 2316 96-97 -0.8594773745914281 2316 98-99 -0.9896609655928685 2316 100-101 -1.4811746032063695 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 7.0 4 25.0 5 42.0 6 132.0 7 319.0 8 785.0 9 1532.0 10 2898.0 11 4786.0 12 6693.0 13 8762.0 14 10903.0 15 13564.0 16 15904.0 17 18338.0 18 16278.0 19 12908.0 20 12361.0 21 12978.0 22 14669.0 23 16797.0 24 20650.0 25 26459.0 26 33922.0 27 43754.0 28 56258.0 29 70745.0 30 86501.0 31 105198.0 32 127004.0 33 154802.0 34 191463.0 35 239346.0 36 314004.0 37 437008.0 38 432387.0 39 82091.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.27335906442224 21.77436496868484 14.987182677229145 22.96509328966378 2 14.809150999516232 22.316266703290005 40.763203112160774 22.11137918503299 3 18.03206784520344 27.02698115230992 31.563419325421044 23.377531677065598 4 12.249903298067236 18.79731004397861 40.229163293530654 28.723623364423506 5 11.899228932989539 38.90088617927578 36.63644583937056 12.563439048364126 6 28.535547486337386 40.53145227024878 18.28909772159243 12.643902521821415 7 24.82881078514235 33.944822121762584 23.766320887148012 17.46004620594706 8 23.765947753591128 37.86030359994029 21.018187373109445 17.355561273359147 9 24.48451341430108 17.03431845289989 21.69468288490411 36.78648524789492 10-11 23.006891927965352 27.89153526509967 29.952941548612277 19.1486312583227 12-13 23.86170933392174 25.250953976147894 30.12714924306834 20.760187446862023 14-15 20.94801235038429 27.230020506822495 27.697422528495842 24.124544614297374 16-17 20.358653369892778 30.317442989027537 28.415547072338626 20.90835656874106 18-19 20.18110888058055 29.15768496644689 30.614494882552535 20.04671127042003 20-21 20.844629573337738 28.320284472369796 30.808106135536363 20.026979818756104 22-23 20.446931555143053 28.144501877387963 30.67249007010574 20.73607649736325 24-25 20.43318053412985 28.327066675770585 30.741340381924715 20.498412408174854 26-27 20.468675920700214 28.484738482832796 30.568940595369487 20.477645001097507 28-29 20.477768500307544 28.618603970255773 30.55232965764206 20.351297871794618 30-31 20.831875401935772 28.306231562070455 30.511877624035407 20.35001541195837 32-33 20.564963729518396 28.535479711410357 30.375356367465255 20.524200191605992 34-35 20.69753961035391 28.49568908560775 30.492762737187096 20.314008566851243 36-37 20.75178520246439 28.368604045160197 30.514762469992668 20.36484828238275 38-39 20.524415017100658 28.648133817948402 30.494314311086146 20.333136853864794 40-41 20.760151520324257 28.540738301855967 30.3890872036538 20.31002297416597 42-43 20.679432796492303 28.571092374079626 30.15161949294275 20.59785533648532 44-45 20.67381538544037 28.751510105919113 30.09766717931021 20.477007329330302 46-47 20.749869407778743 28.706832230083045 29.82485210479167 20.718446257346535 48-49 20.77016484104717 28.879194689130287 29.718339880615385 20.63230058920716 50-51 20.80055821801536 29.020399653026768 29.53195135341009 20.647090775547777 52-53 20.91234657251633 28.98471189888983 29.40505164648516 20.69788988210868 54-55 20.83680653953687 28.90670831491835 29.588283033976882 20.668202111567904 56-57 20.8318496809006 29.045163087319004 29.426392643295753 20.696594588484647 58-59 20.74924662696648 28.978524359206105 29.607979802792876 20.66424921103454 60-61 20.833168381553648 29.07642516889292 29.58831318732993 20.502093262223507 62-63 20.807001839587578 29.075421157287156 29.489623198717023 20.627953804408243 64-65 20.62632830215529 28.930169589575904 29.566633204537652 20.876868903731154 66-67 20.71241404021573 29.08596974277472 29.381553638924725 20.82006257808483 68-69 20.733347100534598 29.081182401494903 29.43744740619741 20.748023091773092 70-71 20.774826584672635 28.788326677182717 29.459367965680983 20.977478772463662 72-73 20.79200862704157 28.946007964790173 29.391694656512147 20.870288751656112 74-75 20.69363084275169 29.165142170691468 29.245279735020034 20.89594725153681 76-77 20.66772679588699 29.11102212278894 29.251932468171 20.969318613153078 78-79 20.651957960944557 29.096360141210365 29.28193889191296 20.969743005932116 80-81 20.69166177237847 29.13697545526524 29.19829148355278 20.973071288803506 82-83 20.86715776057475 29.080398625471975 29.23377546186641 20.818668152086868 84-85 20.850975269913917 29.09698616059511 29.09847132362358 20.95356724586739 86-87 20.69345154973869 29.157472800299967 29.102251738605133 21.046823911356213 88-89 20.755439365568726 29.065415116888204 29.106652532532813 21.072492985010257 90-91 20.76864468242701 29.01717100796247 29.294449416702673 20.919734892907847 92-93 20.783969433398596 29.04562130078205 29.1340909779256 21.036318287893756 94-95 20.615082411414384 29.066929811228754 29.125161347067667 21.192826430289195 96-97 20.646219632508874 28.952255856992277 29.198908694281062 21.20261581621779 98-99 20.5240851009988 28.99385412535856 29.13143422772294 21.3506265459197 100-101 20.549718629113517 29.009920889030163 29.12335262630829 21.317007855548027 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 123.0 1 93.5 2 112.0 3 175.0 4 162.0 5 148.5 6 274.0 7 603.5 8 1007.5 9 1290.5 10 1295.0 11 1241.5 12 1355.5 13 1566.0 14 1846.5 15 2127.0 16 2443.5 17 2793.5 18 3218.5 19 3738.5 20 4346.0 21 5232.5 22 6456.5 23 8153.5 24 10746.0 25 14370.5 26 19254.5 27 24810.0 28 30706.0 29 37807.0 30 45102.5 31 52364.5 32 60986.0 33 68791.5 34 75513.5 35 81819.5 36 89656.0 37 98185.5 38 102906.0 39 104999.0 40 106696.5 41 107887.0 42 107974.5 43 108550.5 44 109762.5 45 110447.0 46 109092.0 47 106251.0 48 103047.0 49 97964.5 50 90048.0 51 80996.5 52 73115.0 53 65520.5 54 57040.5 55 47793.5 56 38280.0 57 29421.5 58 22436.5 59 17119.5 60 12393.5 61 8649.5 62 5898.5 63 3634.0 64 2072.5 65 1127.5 66 569.0 67 322.5 68 176.5 69 91.0 70 55.5 71 34.5 72 23.5 73 16.0 74 10.5 75 8.0 76 8.0 77 5.5 78 3.0 79 2.5 80 2.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0043590499277092075 2 0.005940652113869185 3 0.002083085806161922 4 0.17200888166066686 5 0.002160237132316067 6 5.786349461560895E-4 7 3.8575663077072635E-5 8 5.786349461560895E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.6646879923219E-4 24-25 6.557862723102348E-4 26-27 0.002661720752318012 28-29 2.507418100009721E-4 30-31 0.006364984407716984 32-33 0.020483677093925567 34-35 0.01313501327774323 36-37 9.643915769268157E-4 38-39 3.857566307707263E-4 40-41 0.0018709196592380226 42-43 0.011302669281582281 44-45 0.007271512490028192 46-47 0.01041542903080961 48-49 0.010241838546962784 50-51 0.1847195626445623 52-53 0.11472402199121401 54-55 0.01706973091160464 56-57 0.006519287060025275 58-59 0.017918395499300237 60-61 0.12218841279662757 62-63 0.23648810249399377 64-65 0.24302667738555758 66-67 0.25376999955252233 68-69 0.24889017817327264 70-71 0.23544655959091282 72-73 0.16074478804216166 74-75 0.26054002842254853 76-77 0.20067059932693185 78-79 0.005670622472329677 80-81 1.9287831538536318E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 7.715132615414527E-5 90-91 0.00570919813540675 92-93 0.017725517183914876 94-95 0.0027002964153950842 96-97 1.9287831538536318E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2592308.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.62854055271787 #Duplication Level Percentage of deduplicated Percentage of total 1 76.26753323020118 47.002567645332114 2 14.556541523496305 17.94196819176227 3 4.275725832004133 7.905202284899102 4 1.7824328572107146 4.393949424924292 5 0.9128241189902115 2.812800911734361 6 0.5272141114620466 1.9494861748922312 7 0.34584461122138405 1.4919729053317208 8 0.2366993299297185 1.1669947402699843 9 0.17946679510542043 0.9954248994018642 >10 0.8476793144650077 9.146924691803122 >50 0.045988940671942216 1.928522018924659 >100 0.020918820223584794 2.2939262275539627 >500 8.164830688449625E-4 0.3017184262032113 >1k 1.884191697199397E-4 0.21687342423498834 >5k 1.2561277981329314E-4 0.4516680327321219 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6256 0.24132934821016636 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5413 0.20881006423619417 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3084 0.118967344929692 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.857566307707263E-5 0.0 10-11 0.0 0.0 0.0 9.643915769268157E-5 0.0 12-13 0.0 0.0 0.0 1.1572698923121789E-4 0.0 14-15 0.0 0.0 0.0 1.1572698923121789E-4 0.0 16-17 0.0 0.0 0.0 1.1572698923121789E-4 0.0 18-19 0.0 0.0 0.0 1.1572698923121789E-4 0.0 20-21 0.0 0.0 0.0 1.350148207697542E-4 0.0 22-23 0.0 0.0 0.0 2.507418100009721E-4 0.0 24-25 0.0 0.0 0.0 3.857566307707263E-4 0.0 26-27 0.0 0.0 0.0 5.014836200019443E-4 0.0 28-29 0.0 0.0 0.0 0.0010029672400038883 0.0 30-31 0.0 0.0 0.0 0.0024688424369326487 0.0 32-33 0.0 0.0 0.0 0.00586350078771504 0.0 34-35 0.0 0.0 0.0 0.012344212184663242 0.0 36-37 0.0 0.0 0.0 0.02144806867085238 0.0 38-39 0.0 0.0 0.0 0.0364347137762951 0.0 40-41 0.0 0.0 0.0 0.05396735264482461 0.0 42-43 0.0 0.0 0.0 0.07107565921950632 0.0 44-45 0.0 0.0 0.0 0.09300592367882211 0.0 46-47 0.0 0.0 0.0 0.1201824783166198 0.0 48-49 0.0 0.0 0.0 0.15058010082135304 0.0 50-51 0.0 0.0 0.0 0.18275220382763158 0.0 52-53 0.0 0.0 0.0 0.21625516721006915 0.0 54-55 0.0 0.0 0.0 0.2541750440148316 0.0 56-57 0.0 0.0 0.0 0.2987106470373119 0.0 58-59 0.0 0.0 0.0 0.3486082672275054 0.0 60-61 0.0 0.0 0.0 0.40591241472849676 0.0 62-63 0.0 0.0 0.0 0.4658397073187291 0.0 64-65 0.0 0.0 0.0 0.5284094328297408 0.0 66-67 0.0 0.0 0.0 0.5970741131069301 0.0 68-69 0.0 0.0 0.0 0.677446507128011 0.0 70-71 0.0 0.0 0.0 0.7688129651260576 0.0 72-73 0.0 0.0 0.0 0.8662165143956659 0.0 74-75 0.0 0.0 0.0 0.9702936533776079 0.0 76-77 0.0 0.0 0.0 1.084940524042668 0.0 78-79 0.0 0.0 0.0 1.215056235601634 0.0 80-81 0.0 0.0 0.0 1.360351470581428 0.0 82-83 0.0 0.0 0.0 1.5162164372443399 0.0 84-85 0.0 0.0 0.0 1.6801630053218986 0.0 86-87 0.0 0.0 0.0 1.8540042309787261 0.0 88-89 0.0 0.0 0.0 2.049312813137945 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3075 0.0 54.714317 1 GTATCAA 6070 0.0 42.43785 1 ATCAACG 7625 0.0 33.34442 3 TCAACGC 7640 0.0 33.278957 4 CAACGCA 7790 0.0 32.521225 5 TATCAAC 8065 0.0 31.525866 2 AACGCAG 8160 0.0 31.395449 6 CGCAGAG 9465 0.0 26.866262 8 ACGCAGA 9485 0.0 26.759596 7 GCAGAGT 10950 0.0 22.39956 9 GTACATG 6185 0.0 21.515978 1 TACATGG 6115 0.0 21.216993 2 ACATGGG 5835 0.0 20.931557 3 AGAGTAC 10270 0.0 20.880028 10-11 GAGTACT 8345 0.0 19.272429 12-13 GTGGTAT 1735 0.0 19.175264 1 CAGAGTA 10760 0.0 18.672575 10-11 AGTACTT 8980 0.0 18.570007 12-13 GTACTTT 8915 0.0 17.853947 14-15 TGGTATC 1775 0.0 17.403341 2 >>END_MODULE