FastQCFastQC Report
Sat 18 Jun 2016
SRR1931049_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931049_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2217969
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN983214.43292940523515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20600.058.969291
GTATCAA41300.049.0220831
ATCAACG50900.038.809243
TCAACGC52450.037.6597984
CAACGCA53200.037.3953255
TATCAAC53300.036.4453242
AACGCAG56750.035.0544976
ACGCAGA66950.030.0865927
CGCAGAG68000.029.6348848
GCAGAGT72200.026.9679839
GTGGTAT10100.024.8667741
GTGTAGC7750.023.809241
TGGTATC9900.021.7442232
GAGTACT48350.021.49753612-13
AGAGTAC65650.021.38660410-11
CAGAGTA70100.021.30324210-11
GTACATG41650.021.2900091
TACATGG42600.020.5738852
AGTACTT51600.020.09700212-13
ACATGGG43250.019.8074993
Produced by FastQC (version 0.11.5)