##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931049_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2217969 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.630083648599236 28.0 2.0 31.0 2.0 33.0 2 19.362971258840858 27.0 2.0 31.0 2.0 33.0 3 19.97424580776377 27.0 2.0 31.0 2.0 33.0 4 21.50531274332509 30.0 2.0 35.0 2.0 37.0 5 20.872858457444625 28.0 2.0 35.0 2.0 37.0 6 21.796416451266904 29.0 2.0 35.0 2.0 37.0 7 21.948225155536438 29.0 2.0 35.0 2.0 37.0 8 22.332251713166414 30.0 2.0 35.0 2.0 37.0 9 23.544409773085196 32.0 2.0 37.0 2.0 39.0 10-11 23.841247104896418 32.0 2.0 37.0 2.0 39.0 12-13 23.70587280525562 32.0 2.0 37.0 2.0 39.0 14-15 23.63116301445151 32.0 2.0 38.0 2.0 40.0 16-17 22.52368495682311 30.0 2.0 37.0 2.0 40.0 18-19 21.54550401741413 27.0 2.0 37.0 2.0 39.5 20-21 21.211526851818036 27.0 2.0 37.0 2.0 39.5 22-23 20.84998956252319 27.0 2.0 37.0 2.0 39.0 24-25 20.673840572163094 26.0 2.0 36.5 2.0 39.0 26-27 20.378219217671663 25.0 2.0 36.0 2.0 39.0 28-29 20.156035093366953 24.0 2.0 36.0 2.0 39.0 30-31 20.09333042977607 24.5 2.0 36.0 2.0 39.0 32-33 19.920809082543535 24.5 2.0 36.0 2.0 39.0 34-35 19.566259041492465 24.0 2.0 35.5 2.0 39.0 36-37 19.11573403415467 21.0 2.0 35.0 2.0 38.5 38-39 18.957464689542547 20.0 2.0 35.0 2.0 38.0 40-41 18.781286618523524 19.0 2.0 35.0 2.0 38.0 42-43 18.607886990305097 18.5 2.0 35.0 2.0 38.0 44-45 18.193603246934472 16.5 2.0 34.0 2.0 38.0 46-47 17.83478871886848 15.0 2.0 34.0 2.0 38.0 48-49 17.54562529954206 14.0 2.0 33.5 2.0 38.0 50-51 15.469049161642927 7.0 2.0 31.5 2.0 37.5 52-53 17.263756166114135 13.5 2.0 33.5 2.0 38.0 54-55 17.94799882234603 15.0 2.0 35.0 2.0 38.5 56-57 17.895548134351742 13.5 2.0 35.0 2.0 39.0 58-59 17.618763833038244 10.5 2.0 34.5 2.0 38.5 60-61 17.33036349921933 8.0 2.0 34.0 2.0 38.0 62-63 16.889519646126704 7.5 2.0 34.0 2.0 38.0 64-65 16.531259904894974 4.5 2.0 33.5 2.0 38.0 66-67 16.168121150475955 2.0 2.0 33.0 2.0 38.0 68-69 15.71561257168157 2.0 2.0 32.0 2.0 37.0 70-71 15.364322945902309 2.0 2.0 32.0 2.0 36.5 72-73 14.958441484078453 2.0 2.0 31.0 2.0 36.0 74-75 14.505340245963762 2.0 2.0 31.0 2.0 35.5 76-77 14.085690557442417 2.0 2.0 30.5 2.0 35.0 78-79 13.692243895203223 2.0 2.0 30.0 2.0 35.0 80-81 13.257626459161512 2.0 2.0 29.5 2.0 34.0 82-83 12.803303607940418 2.0 2.0 29.0 2.0 34.0 84-85 12.3973786378439 2.0 2.0 29.0 2.0 34.0 86-87 11.926043375718958 2.0 2.0 27.5 2.0 34.0 88-89 11.497589686781016 2.0 2.0 26.5 2.0 34.0 90-91 11.016240082706297 2.0 2.0 26.0 2.0 33.5 92-93 10.483140431629117 2.0 2.0 25.0 2.0 33.0 94-95 9.927227116339317 2.0 2.0 23.0 2.0 32.0 96-97 9.312877456808458 2.0 2.0 19.0 2.0 32.0 98-99 8.675301818916314 2.0 2.0 2.0 2.0 32.0 100-101 7.83750043395557 2.0 2.0 2.0 2.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.679552922982012 1101 2 9.221230277448239 1101 3 8.641044886837886 1101 4 10.71525311847288 1101 5 11.525868964555553 1101 6 10.656725854043888 1101 7 10.383250424340332 1101 8 9.548779245754066 1101 9 9.678442326679505 1101 10-11 9.113374853169756 1101 12-13 9.013873820558967 1101 14-15 9.88100319178118 1101 16-17 10.69194463922987 1101 18-19 11.478329865905849 1101 20-21 11.538805134321368 1101 22-23 11.480971435993098 1101 24-25 11.433421867132601 1101 26-27 11.561585921326799 1101 28-29 11.563196714585665 1101 30-31 11.059852250308264 1101 32-33 10.883307494639023 1101 34-35 10.737116158449101 1101 36-37 11.376989912751458 1101 38-39 10.96162472767725 1101 40-41 10.618377172428058 1101 42-43 10.254366413533276 1101 44-45 10.028586314933321 1101 46-47 9.874247527153056 1101 48-49 10.210964663852927 1101 50-51 11.645939517104608 1101 52-53 11.069897742877206 1101 54-55 10.736632982121641 1101 56-57 10.850347663093725 1101 58-59 10.747175178386872 1101 60-61 10.242489580829861 1101 62-63 10.437036655960373 1101 64-65 10.325152455899726 1101 66-67 9.91677731599969 1101 68-69 9.831420058824683 1101 70-71 9.45700765213134 1101 72-73 9.38207285598252 1101 74-75 9.149726422747767 1101 76-77 9.092108252136379 1101 78-79 8.39621933647751 1101 80-81 8.154329364289474 1101 82-83 7.8826162584467365 1101 84-85 7.807131037473702 1101 86-87 7.672638871879824 1101 88-89 7.2633012867857225 1101 90-91 6.88742424662548 1101 92-93 6.406985877897137 1101 94-95 6.1361508432026675 1101 96-97 5.989262564084287 1101 98-99 5.906156227323539 1101 100-101 5.133401233276388 1102 1 7.768886415735057 1102 2 8.964380448919322 1102 3 8.521176825628306 1102 4 10.428064390205677 1102 5 11.127547426383074 1102 6 10.141555929711316 1102 7 10.10564946326721 1102 8 9.583947803631965 1102 9 9.471688624441366 1102 10-11 8.653914147282048 1102 12-13 8.8495562223308 1102 14-15 9.28236670478432 1102 16-17 10.033994055544962 1102 18-19 11.085676078074506 1102 20-21 11.182170617296673 1102 22-23 11.383247057492746 1102 24-25 10.859165303879983 1102 26-27 11.051119163183586 1102 28-29 10.916698729157385 1102 30-31 11.078082179081814 1102 32-33 10.94529490807317 1102 34-35 10.855786958641165 1102 36-37 10.664258540660157 1102 38-39 10.711525564820523 1102 40-41 10.339177364490855 1102 42-43 10.096893329843624 1102 44-45 10.13440314168395 1102 46-47 9.972780296444263 1102 48-49 9.66168935929485 1102 50-51 11.45978435997181 1102 52-53 10.80155356330988 1102 54-55 10.33782559339755 1102 56-57 10.31221313942654 1102 58-59 10.279199656904115 1102 60-61 9.960480190249665 1102 62-63 10.017684526283826 1102 64-65 9.731813732661838 1102 66-67 9.512244197503401 1102 68-69 9.25047365611151 1102 70-71 8.99822856040617 1102 72-73 8.733125690332157 1102 74-75 8.321176345030656 1102 76-77 8.014823758170605 1102 78-79 7.674462721033914 1102 80-81 7.466063224467 1102 82-83 7.322557823530781 1102 84-85 7.388882319948355 1102 86-87 7.273760788104765 1102 88-89 6.789683351308952 1102 90-91 6.4402129528775784 1102 92-93 6.296924216629863 1102 94-95 5.962912004005915 1102 96-97 5.826482606937825 1102 98-99 5.290089567109574 1102 100-101 4.8010092353868 1103 1 7.744405280531822 1103 2 9.015974223489028 1103 3 8.465075960774705 1103 4 10.476203486703636 1103 5 11.03183001469192 1103 6 10.338500997772357 1103 7 9.831057971607883 1103 8 9.41572193759428 1103 9 9.129911364502181 1103 10-11 8.67600343654858 1103 12-13 8.719769925476673 1103 14-15 9.254233386607236 1103 16-17 10.200977108961279 1103 18-19 11.134760545758532 1103 20-21 11.630274191330226 1103 22-23 11.616775053931022 1103 24-25 11.396296225474806 1103 26-27 11.133038228800682 1103 28-29 11.023429999345982 1103 30-31 10.759157230944613 1103 32-33 11.033956611067477 1103 34-35 10.854531357932608 1103 36-37 11.032552609829501 1103 38-39 10.424102411751857 1103 40-41 10.235764839743538 1103 42-43 9.748193452040901 1103 44-45 9.9642121729153 1103 46-47 9.61801033992376 1103 48-49 9.629039004543838 1103 50-51 11.202884002852635 1103 52-53 10.5781902016533 1103 54-55 9.992281057699842 1103 56-57 9.96734210965398 1103 58-59 9.740767665846302 1103 60-61 9.72128280849211 1103 62-63 9.465033129540526 1103 64-65 9.255063422432311 1103 66-67 8.985661553305876 1103 68-69 8.925965523835323 1103 70-71 8.681060790174945 1103 72-73 8.61592523943367 1103 74-75 8.10948945424092 1103 76-77 7.8951021595649795 1103 78-79 7.3807506708667265 1103 80-81 7.201736304122573 1103 82-83 7.198228724475209 1103 84-85 6.8439435606769905 1103 86-87 6.610930073961141 1103 88-89 6.4384807974805796 1103 90-91 6.181150461758195 1103 92-93 5.829803809684508 1103 94-95 5.5461979740533724 1103 96-97 5.150975582962529 1103 98-99 4.8514070242694824 1103 100-101 4.251437873123972 1104 1 7.7952385446188295 1104 2 9.351831816117524 1104 3 8.80508279307858 1104 4 10.768066582106606 1104 5 11.306329825418636 1104 6 10.595882194164162 1104 7 10.321792813587773 1104 8 9.794644280151225 1104 9 9.522535246174062 1104 10-11 8.80693623068387 1104 12-13 8.726116581802554 1104 14-15 9.434849032583106 1104 16-17 10.339506686374047 1104 18-19 11.05475657563602 1104 20-21 11.19482884514478 1104 22-23 11.383893668265937 1104 24-25 11.402192319058901 1104 26-27 11.507935975063688 1104 28-29 11.222304515775829 1104 30-31 10.933181088611104 1104 32-33 10.823471794477904 1104 34-35 10.798101606019099 1104 36-37 10.91834095613498 1104 38-39 10.3838582953811 1104 40-41 10.15002727407494 1104 42-43 10.144939798143255 1104 44-45 10.089140387560434 1104 46-47 9.903395672629262 1104 48-49 9.763146973410695 1104 50-51 11.292038182099049 1104 52-53 10.704855114079784 1104 54-55 10.304815565450813 1104 56-57 10.372665766967774 1104 58-59 10.569900517225228 1104 60-61 10.165357169810193 1104 62-63 9.976857631659467 1104 64-65 9.769070568652847 1104 66-67 9.56172910078577 1104 68-69 9.387141418728639 1104 70-71 9.066561071776656 1104 72-73 8.832799645069398 1104 74-75 8.490834956461418 1104 76-77 8.16788419983864 1104 78-79 8.021347481842726 1104 80-81 7.903898174327264 1104 82-83 7.900921021514234 1104 84-85 7.440293356169519 1104 86-87 7.075391014418534 1104 88-89 6.803900568690729 1104 90-91 6.580845316294754 1104 92-93 6.123164010591177 1104 94-95 5.694245227744203 1104 96-97 5.524786623596272 1104 98-99 5.02339550168133 1104 100-101 4.639890817939268 1105 1 7.772531488610898 1105 2 8.951380628227195 1105 3 8.692971697423179 1105 4 10.473662381957517 1105 5 10.456795101784593 1105 6 10.304631315863624 1105 7 9.79242422696505 1105 8 9.603612754066145 1105 9 9.508490284319944 1105 10-11 8.899145838667582 1105 12-13 8.588279037976694 1105 14-15 9.164512763853455 1105 16-17 10.062013472025914 1105 18-19 10.583861226259657 1105 20-21 10.941285861027303 1105 22-23 11.30730889309304 1105 24-25 11.276117582370727 1105 26-27 11.338458746677016 1105 28-29 10.96786807263442 1105 30-31 10.819521508982678 1105 32-33 10.832993107309829 1105 34-35 10.512366276758943 1105 36-37 10.48562947741571 1105 38-39 10.47892483221979 1105 40-41 10.353702582862226 1105 42-43 9.908112879524708 1105 44-45 9.709105362540015 1105 46-47 9.65598171041491 1105 48-49 9.38504420957278 1105 50-51 10.586191286378735 1105 52-53 9.773997785754101 1105 54-55 9.607696747884091 1105 56-57 9.515420188787434 1105 58-59 9.488299531388606 1105 60-61 9.327296707066186 1105 62-63 9.34781593333831 1105 64-65 9.183875773185417 1105 66-67 8.961847158644824 1105 68-69 8.574878738208902 1105 70-71 8.592333173741553 1105 72-73 8.282694070669764 1105 74-75 7.981243201251358 1105 76-77 7.776490296760221 1105 78-79 7.4652808772366885 1105 80-81 7.094799872434415 1105 82-83 7.115163935367663 1105 84-85 6.592413404248667 1105 86-87 6.556209109014626 1105 88-89 6.390281471137843 1105 90-91 6.226073246430181 1105 92-93 5.823808870437594 1105 94-95 5.6284272579827075 1105 96-97 5.473065511213036 1105 98-99 5.021734844908698 1105 100-101 4.446869785093504 1106 1 7.69517253271885 1106 2 8.915432497645789 1106 3 8.356533716799152 1106 4 10.189334902638983 1106 5 11.086351413094036 1106 6 10.129867618023003 1106 7 9.075869940464429 1106 8 8.88545792142115 1106 9 8.33879825899286 1106 10-11 8.24645416540346 1106 12-13 8.129063923931529 1106 14-15 8.653709702871506 1106 16-17 9.604597095915441 1106 18-19 10.67149103830807 1106 20-21 10.637385557395337 1106 22-23 10.703608479016957 1106 24-25 10.388401184278656 1106 26-27 10.816824388191133 1106 28-29 10.694187906646562 1106 30-31 10.278942946221651 1106 32-33 10.370790730080618 1106 34-35 10.546701083276218 1106 36-37 10.626958631267225 1106 38-39 10.234982727812568 1106 40-41 10.140511301871303 1106 42-43 9.82594799610759 1106 44-45 9.502373543375281 1106 46-47 9.29262324419419 1106 48-49 9.195234109118672 1106 50-51 11.01865881884627 1106 52-53 10.313624438171452 1106 54-55 9.69784774179179 1106 56-57 9.571903061907161 1106 58-59 9.205963977854484 1106 60-61 9.17605957210812 1106 62-63 9.11649153323037 1106 64-65 9.101379825483743 1106 66-67 8.861771027262785 1106 68-69 8.450347812962413 1106 70-71 7.9991597997095045 1106 72-73 7.975056627611929 1106 74-75 7.538406789005284 1106 76-77 7.5353059891179495 1106 78-79 7.155938609486549 1106 80-81 6.907953891939059 1106 82-83 6.649493291163923 1106 84-85 6.4419931625940805 1106 86-87 6.364352754278128 1106 88-89 6.031584086232943 1106 90-91 5.6979749583140045 1106 92-93 5.409065563654931 1106 94-95 5.110828041456836 1106 96-97 4.604188234643452 1106 98-99 4.36846468036841 1106 100-101 3.786038934369987 1107 1 7.263894210115499 1107 2 8.11885583551516 1107 3 8.110744848571485 1107 4 9.854198649087742 1107 5 10.532697692261067 1107 6 9.661763743645604 1107 7 9.603077589302611 1107 8 8.209595389003514 1107 9 5.4599921653170504 1107 10-11 7.507843112503355 1107 12-13 7.49484265902251 1107 14-15 7.89965746504873 1107 16-17 8.926397929379196 1107 18-19 10.342492494614888 1107 20-21 10.691852758660122 1107 22-23 10.041431428537841 1107 24-25 10.240246414321199 1107 26-27 10.369044880293746 1107 28-29 10.28712353647052 1107 30-31 10.114673128909903 1107 32-33 10.460644949081928 1107 34-35 10.405279101797046 1107 36-37 10.651841848193275 1107 38-39 10.348258551616244 1107 40-41 10.33255431376492 1107 42-43 10.055540203672429 1107 44-45 9.433287214532886 1107 46-47 9.582401332037694 1107 48-49 9.527880758861407 1107 50-51 11.144893562772353 1107 52-53 10.151067704542967 1107 54-55 9.941164955833525 1107 56-57 9.923178501960283 1107 58-59 9.97683554255757 1107 60-61 9.844609799661587 1107 62-63 10.14269471057883 1107 64-65 9.684951853428561 1107 66-67 9.66301874708598 1107 68-69 9.357978259412853 1107 70-71 9.193075850297374 1107 72-73 9.018187094841489 1107 74-75 8.93511307747429 1107 76-77 8.62907172266951 1107 78-79 8.372374804767832 1107 80-81 7.857772936753875 1107 82-83 7.729457887080786 1107 84-85 7.741972914005075 1107 86-87 7.379507717879298 1107 88-89 7.2321814638196145 1107 90-91 7.248788129269569 1107 92-93 6.673536186842837 1107 94-95 6.253920222053031 1107 96-97 5.62064768485447 1107 98-99 5.193670636813605 1107 100-101 4.7919655814247015 1108 1 6.379873642544805 1108 2 7.360505183679468 1108 3 7.439118183837561 1108 4 8.856664992895858 1108 5 10.526399307026498 1108 6 9.028897261464536 1108 7 8.383454119891454 1108 8 5.5383269113388245 1108 9 6.620000421476476 1108 10-11 5.679457084880237 1108 12-13 4.278528568527928 1108 14-15 6.646359771853987 1108 16-17 7.7661423035575865 1108 18-19 7.451745853143862 1108 20-21 7.6330635990184525 1108 22-23 8.270592285168654 1108 24-25 8.231259262662938 1108 26-27 6.535319747028371 1108 28-29 8.3675468624668 1108 30-31 8.63231804367766 1108 32-33 9.29950558507581 1108 34-35 8.81724622631642 1108 36-37 8.417567809875663 1108 38-39 8.224103052910777 1108 40-41 8.459202678928058 1108 42-43 7.890263919070215 1108 44-45 7.400682299947199 1108 46-47 8.081424895879987 1108 48-49 8.448761419368164 1108 50-51 9.933377795976298 1108 52-53 9.262699435715692 1108 54-55 8.744045298068492 1108 56-57 8.708435725268213 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70-71 -13.05263724045963 2313 72-73 -12.654753097546633 2313 74-75 -12.210641836787525 2313 76-77 -11.822622129275283 2313 78-79 -11.440255893684698 2313 80-81 -11.014456256052483 2313 82-83 -10.573762522789563 2313 84-85 -10.163715082649048 2313 86-87 -9.703511939167962 2313 88-89 -9.290403228777627 2313 90-91 -8.821475358810732 2313 92-93 -8.308270588564943 2313 94-95 -7.749425658331747 2313 96-97 -7.132175401720184 2313 98-99 -6.49432601730601 2313 100-101 -5.688168253790689 2314 1 -18.273537826689186 2314 2 -17.031290546751674 2314 3 -17.631204126752646 2314 4 -19.091849029707742 2314 5 -18.4438811450455 2314 6 -19.379223291067905 2314 7 -19.556688199070454 2314 8 -19.96020804052388 2314 9 -21.16986981711708 2314 10-11 -21.485046891678994 2314 12-13 -21.367976245201664 2314 14-15 -21.2862480138304 2314 16-17 -20.148257026706123 2314 18-19 -19.16064426824584 2314 20-21 -18.834496049369992 2314 22-23 -18.44121181696613 2314 24-25 -18.274600929886248 2314 26-27 -17.996283434219013 2314 28-29 -17.77853936608071 2314 30-31 -17.712177561177928 2314 32-33 -17.558384745606485 2314 34-35 -17.189451136596933 2314 36-37 -16.757819381417765 2314 38-39 -16.597998244703287 2314 40-41 -16.448917873604383 2314 42-43 -16.271937838987547 2314 44-45 -15.870142312861507 2314 46-47 -15.50124567758171 2314 48-49 -15.214068216462724 2314 50-51 -13.19952299933555 2314 52-53 -14.943035181278866 2314 54-55 -15.599403843831887 2314 56-57 -15.56657473936134 2314 58-59 -15.288116360411902 2314 60-61 -14.99687911710964 2314 62-63 -14.56997781137851 2314 64-65 -14.21320791235981 2314 66-67 -13.85810212284292 2314 68-69 -13.4094830538452 2314 70-71 -13.05263724045963 2314 72-73 -12.654753097546633 2314 74-75 -12.210641836787525 2314 76-77 -11.822622129275283 2314 78-79 -11.440255893684698 2314 80-81 -11.014456256052483 2314 82-83 -10.573762522789563 2314 84-85 -10.163715082649048 2314 86-87 -9.703511939167962 2314 88-89 -9.290403228777627 2314 90-91 -8.821475358810732 2314 92-93 -8.308270588564943 2314 94-95 -7.749425658331747 2314 96-97 -7.132175401720184 2314 98-99 -6.49432601730601 2314 100-101 -5.688168253790689 2315 1 -18.273537826689186 2315 2 -17.031290546751674 2315 3 -17.631204126752646 2315 4 -19.091849029707742 2315 5 -18.4438811450455 2315 6 -19.379223291067905 2315 7 -19.556688199070454 2315 8 -19.96020804052388 2315 9 -21.16986981711708 2315 10-11 -21.485046891678994 2315 12-13 -21.367976245201664 2315 14-15 -21.2862480138304 2315 16-17 -20.148257026706123 2315 18-19 -19.16064426824584 2315 20-21 -18.834496049369992 2315 22-23 -18.44121181696613 2315 24-25 -18.274600929886248 2315 26-27 -17.996283434219013 2315 28-29 -17.77853936608071 2315 30-31 -17.712177561177928 2315 32-33 -17.558384745606485 2315 34-35 -17.189451136596933 2315 36-37 -16.757819381417765 2315 38-39 -16.597998244703287 2315 40-41 -16.448917873604383 2315 42-43 -16.271937838987547 2315 44-45 -15.870142312861507 2315 46-47 -15.50124567758171 2315 48-49 -15.214068216462724 2315 50-51 -13.19952299933555 2315 52-53 -14.943035181278866 2315 54-55 -15.599403843831887 2315 56-57 -15.56657473936134 2315 58-59 -15.288116360411902 2315 60-61 -14.99687911710964 2315 62-63 -14.56997781137851 2315 64-65 -14.21320791235981 2315 66-67 -13.85810212284292 2315 68-69 -13.4094830538452 2315 70-71 -13.05263724045963 2315 72-73 -12.654753097546633 2315 74-75 -12.210641836787525 2315 76-77 -11.822622129275283 2315 78-79 -11.440255893684698 2315 80-81 -11.014456256052483 2315 82-83 -10.573762522789563 2315 84-85 -10.163715082649048 2315 86-87 -9.703511939167962 2315 88-89 -9.290403228777627 2315 90-91 -8.821475358810732 2315 92-93 -8.308270588564943 2315 94-95 -7.749425658331747 2315 96-97 -7.132175401720184 2315 98-99 -6.49432601730601 2315 100-101 -5.688168253790689 2316 1 -18.273537826689186 2316 2 -17.031290546751674 2316 3 -17.631204126752646 2316 4 -19.091849029707742 2316 5 -18.4438811450455 2316 6 -19.379223291067905 2316 7 -19.556688199070454 2316 8 -19.96020804052388 2316 9 -21.16986981711708 2316 10-11 -21.485046891678994 2316 12-13 -21.367976245201664 2316 14-15 -21.2862480138304 2316 16-17 -20.148257026706123 2316 18-19 -19.16064426824584 2316 20-21 -18.834496049369992 2316 22-23 -18.44121181696613 2316 24-25 -18.274600929886248 2316 26-27 -17.996283434219013 2316 28-29 -17.77853936608071 2316 30-31 -17.712177561177928 2316 32-33 -17.558384745606485 2316 34-35 -17.189451136596933 2316 36-37 -16.757819381417765 2316 38-39 -16.597998244703287 2316 40-41 -16.448917873604383 2316 42-43 -16.271937838987547 2316 44-45 -15.870142312861507 2316 46-47 -15.50124567758171 2316 48-49 -15.214068216462724 2316 50-51 -13.19952299933555 2316 52-53 -14.943035181278866 2316 54-55 -15.599403843831887 2316 56-57 -15.56657473936134 2316 58-59 -15.288116360411902 2316 60-61 -14.99687911710964 2316 62-63 -14.56997781137851 2316 64-65 -14.21320791235981 2316 66-67 -13.85810212284292 2316 68-69 -13.4094830538452 2316 70-71 -13.05263724045963 2316 72-73 -12.654753097546633 2316 74-75 -12.210641836787525 2316 76-77 -11.822622129275283 2316 78-79 -11.440255893684698 2316 80-81 -11.014456256052483 2316 82-83 -10.573762522789563 2316 84-85 -10.163715082649048 2316 86-87 -9.703511939167962 2316 88-89 -9.290403228777627 2316 90-91 -8.821475358810732 2316 92-93 -8.308270588564943 2316 94-95 -7.749425658331747 2316 96-97 -7.132175401720184 2316 98-99 -6.49432601730601 2316 100-101 -5.688168253790689 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 591347.0 3 26311.0 4 84694.0 5 48203.0 6 30347.0 7 29806.0 8 29247.0 9 29412.0 10 30813.0 11 32558.0 12 33311.0 13 33218.0 14 33990.0 15 34726.0 16 35839.0 17 36009.0 18 34673.0 19 35589.0 20 37327.0 21 38839.0 22 41154.0 23 43861.0 24 45954.0 25 49025.0 26 52412.0 27 55559.0 28 59196.0 29 63054.0 30 67012.0 31 72916.0 32 76883.0 33 81128.0 34 80466.0 35 72792.0 36 49555.0 37 18754.0 38 1976.0 39 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.3814313184952 20.776335423039576 14.184988509221286 22.657244749243937 2 14.846031650022443 20.881056146796002 42.19302629861551 22.079885904566048 3 18.5127335101088 26.693114710734516 30.4937676992484 24.300384079908284 4 11.91518799293552 17.620328868089338 40.21537735668607 30.24910578228907 5 12.203008350900491 38.36454087381113 36.113411175512915 13.31903959977546 6 28.924868612954384 39.44010061283896 18.230726593507537 13.40430418069912 7 25.580910194872423 33.66994427221096 23.361044124105305 17.38810140881131 8 24.197162692287762 37.190824265830855 20.736321008740404 17.87569203314098 9 25.252949178084506 16.974649618167252 21.808505472024407 35.96389573172384 10-11 23.636875405317014 27.6431733619995 29.313539247481067 19.406411985202414 12-13 24.454180979301753 25.21629711296118 29.559876131781017 20.769645775956054 14-15 21.626625777027076 26.837726023515284 27.516052597245 24.019595602212643 16-17 20.75480531625739 29.852784931281978 28.247116254286368 21.145293498174258 18-19 20.472872305969666 28.52567483782608 30.75249314443118 20.24895971177307 20-21 21.346652619857867 27.79538213805846 30.794253081891647 20.063712160192026 22-23 20.894014241119187 27.805095475854593 30.567962248438935 20.73292803458729 24-25 20.758181235753415 28.011000251073863 30.751044801224943 20.479773711947775 26-27 20.698341622434118 28.130388401926787 30.554899109396256 20.616370866242846 28-29 20.66278920588506 28.244140842479315 30.64886447808595 20.444205473549673 30-31 20.889413098627013 27.97617957281322 30.692380127124657 20.442027201435113 32-33 20.723710098275454 28.217914103215413 30.443435344683717 20.61494045382542 34-35 20.944205112293847 28.16141196687272 30.559112389317168 20.335270531516265 36-37 20.983392447137604 28.067888441662408 30.324915800868357 20.623803310331635 38-39 20.893349027412054 28.20039882375307 30.432573489763847 20.47367865907103 40-41 21.079807238768897 28.21745480327821 30.308361774642012 20.394376183310886 42-43 20.984439978931597 28.186248572107587 30.049266445783022 20.780045003177797 44-45 21.04673223877394 28.473960842804985 29.96325873418745 20.51604818423362 46-47 21.151611596335382 28.410381672938406 29.496357503964365 20.941649226761843 48-49 21.12252117405049 28.578673055834525 29.46147774628764 20.837328023827343 50-51 21.253564034542205 28.850676595845176 29.0103516409122 20.885407728700418 52-53 20.829769058083432 29.116737913638957 29.064025172645785 20.989467855631823 54-55 20.738229618181066 29.042550164572827 29.2551851220649 20.964035095181202 56-57 20.835503170550563 29.10694777102561 29.231484114379242 20.826064944044585 58-59 20.75055497542337 29.102218229001142 29.295772628540433 20.851454167035058 60-61 20.790713513163663 29.178691955375037 29.26663082212746 20.76396370933384 62-63 20.746064787591695 29.077958929881724 29.21828375949681 20.957692523029774 64-65 20.528229948723016 28.962589160780734 29.3596406978552 21.149540192641048 66-67 20.53264891267787 29.048070313985285 29.074335687857385 21.34494508547946 68-69 20.74347399853246 28.953593405303778 29.07681721031737 21.22611538584639 70-71 20.80664944291098 28.772776896184688 29.120196572819378 21.30037708808495 72-73 20.79536640562863 28.921096278540098 29.020240068362224 21.263297247469048 74-75 20.665724099086784 29.243874081718534 28.741694709594146 21.348707109600536 76-77 20.74991879937301 29.09538174679135 28.813040099552257 21.341659354283387 78-79 20.626416202858003 29.126784825577996 28.785341792404807 21.4614571791592 80-81 20.651356264931657 29.130443200960364 28.562610303180403 21.655590230927572 82-83 20.87935236061797 28.94370026139655 28.727442605679787 21.449504772305698 84-85 20.92324288832636 28.983130311304194 28.537117081564656 21.556509718804794 86-87 20.734714957892926 29.040397743703856 28.680474887226755 21.544412411176467 88-89 20.915596873439373 28.93082631499262 28.521589447970875 21.631987363597126 90-91 20.825914503567482 28.976337573824217 28.607456407362154 21.590291515246143 92-93 20.860343315747304 28.964068523354985 28.402140574228717 21.773447586668993 94-95 20.709477275989073 28.822754951057693 28.543161481151856 21.924606291801375 96-97 20.72513746016775 28.763705880891216 28.50711542912729 22.004041229813744 98-99 20.604487887293963 28.844725440895402 28.21090365265823 22.3398830191524 100-101 20.898599110770135 28.97510558084102 28.119822267182542 22.006473041206302 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 132719.0 1 120689.5 2 80292.5 3 40542.0 4 25186.5 5 19930.0 6 18599.0 7 18756.0 8 19251.0 9 19078.0 10 17523.5 11 14945.5 12 11973.5 13 9202.0 14 7115.5 15 5851.5 16 5460.0 17 5891.5 18 6752.0 19 7954.0 20 9351.0 21 10879.5 22 12440.5 23 14135.5 24 16050.0 25 18061.0 26 20765.5 27 23856.0 28 26911.0 29 30646.0 30 34829.5 31 38968.0 32 43127.5 33 47037.5 34 50843.5 35 54414.0 36 58292.5 37 62172.0 38 64249.5 39 64811.5 40 65010.5 41 64417.5 42 63651.5 43 63866.0 44 64330.5 45 63805.0 46 62343.5 47 61044.5 48 59416.5 49 57047.0 50 53722.5 51 49840.5 52 45499.0 53 40630.5 54 35718.0 55 30758.0 56 25871.5 57 20991.0 58 16788.0 59 13415.0 60 10501.0 61 7967.5 62 5878.0 63 4200.0 64 2843.0 65 1875.5 66 1185.5 67 745.0 68 488.5 69 311.0 70 191.0 71 131.5 72 91.5 73 61.5 74 39.5 75 28.5 76 20.5 77 12.0 78 10.0 79 8.0 80 4.5 81 3.0 82 2.5 83 3.0 84 3.0 85 1.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.13430395104711 2 29.18679206066451 3 22.958391212861855 4 29.286207336531756 5 32.37245425882869 6 25.003189855223408 7 23.925492195788127 8 19.96263248043593 9 11.801156824103492 10-11 15.42503524620948 12-13 15.511578385450834 14-15 19.65476072929784 16-17 22.958413756008312 18-19 26.917598938488318 20-21 28.979643087888064 22-23 28.58626518224556 24-25 27.09302970420236 26-27 28.55123313265424 28-29 25.49688476259136 30-31 27.390035658749063 32-33 26.38271770254679 34-35 24.4371765340273 36-37 27.40335865830406 38-39 27.547431907298975 40-41 24.582219138319786 42-43 25.44255577963443 44-45 24.157258284493608 46-47 27.441005712884177 48-49 22.828181998936863 50-51 28.406911909048322 52-53 21.99476187448968 54-55 23.147505668474174 56-57 25.24014086761357 58-59 24.527890155362858 60-61 24.06906949556103 62-63 23.9002213286119 64-65 22.426440585959497 66-67 22.926109427138073 68-69 23.93157884533102 70-71 22.420669540466978 72-73 22.282096819207123 74-75 19.591910436980857 76-77 21.84917823468227 78-79 20.95707830001231 80-81 21.992845707040992 82-83 22.840017150825823 84-85 19.797301044333803 86-87 21.345136023091396 88-89 20.191670848420333 90-91 21.311907425216493 92-93 21.305505171623228 94-95 20.36342708126218 96-97 19.259849889696383 98-99 18.661802757387502 100-101 20.31067611855711 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2217969.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.02205499543822 #Duplication Level Percentage of deduplicated Percentage of total 1 89.89891454615476 70.14098054750289 2 6.712723275524707 10.474809291442936 3 1.6563796912506539 3.8770244209225653 4 0.6256823277060505 1.952680839278211 5 0.3238973699569677 1.263556920583017 6 0.20028430570011432 0.9375955868434488 7 0.12431919146954466 0.678974715567766 8 0.09023751627462422 0.5632413165944385 9 0.05825108709470722 0.4090382568762578 >10 0.2933132221383 3.8559292530650833 >50 0.011845126045653237 0.6260818403396284 >100 0.003918764173879613 0.5168974181752123 >500 0.0 0.0 >1k 1.7518238258097045E-4 0.22365550432139042 >5k 0.0 0.0 >10k+ 5.8394127526990145E-5 4.479534088487151 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 98321 4.43292940523515 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 4.50862929103157E-5 0.0 7 0.0 0.0 0.0 4.50862929103157E-5 0.0 8 0.0 0.0 0.0 4.50862929103157E-5 0.0 9 0.0 0.0 0.0 4.50862929103157E-5 0.0 10-11 0.0 0.0 0.0 4.50862929103157E-5 0.0 12-13 0.0 0.0 0.0 4.50862929103157E-5 0.0 14-15 0.0 0.0 0.0 4.50862929103157E-5 0.0 16-17 0.0 0.0 0.0 4.50862929103157E-5 0.0 18-19 0.0 0.0 0.0 4.50862929103157E-5 0.0 20-21 0.0 0.0 0.0 4.50862929103157E-5 0.0 22-23 0.0 0.0 0.0 9.01725858206314E-5 0.0 24-25 0.0 0.0 0.0 1.352588787309471E-4 0.0 26-27 0.0 0.0 0.0 2.254314645515785E-4 0.0 28-29 0.0 0.0 0.0 4.7340607555831487E-4 0.0 30-31 0.0 0.0 0.0 0.001487847666040418 0.0 32-33 0.0 0.0 0.0 0.0039675937761077815 0.0 34-35 0.0 0.0 0.0 0.007980273845125879 0.0 36-37 0.0 0.0 0.0 0.014450156877756182 0.0 38-39 0.0 0.0 0.0 0.024910176832949424 0.0 40-41 0.0 0.0 0.0 0.0347615318338534 0.0 42-43 0.0 0.0 0.0 0.04562732842523949 0.0 44-45 0.0 0.0 0.0 0.060145114742361144 0.0 46-47 0.0 0.0 0.0 0.07935187552215564 0.0 48-49 0.0 0.0 0.0 0.10000139767508022 0.0 50-51 0.0 2.254314645515785E-5 0.0 0.12468614304347805 0.0 52-53 0.0 4.50862929103157E-5 0.0 0.15119688327474368 0.0 54-55 0.0 4.50862929103157E-5 0.0 0.18160758784275163 0.0 56-57 0.0 4.50862929103157E-5 0.0 0.21812748510010735 0.0 58-59 0.0 4.50862929103157E-5 0.0 0.25947161569886684 0.0 60-61 0.0 4.50862929103157E-5 0.0 0.3045353654627274 2.254314645515785E-5 62-63 0.0 4.50862929103157E-5 0.0 0.3521690339224759 4.50862929103157E-5 64-65 0.0 4.50862929103157E-5 0.0 0.402169732760016 4.50862929103157E-5 66-67 0.0 4.50862929103157E-5 0.0 0.4557547918839262 4.50862929103157E-5 68-69 0.0 4.50862929103157E-5 0.0 0.5199125866953055 4.50862929103157E-5 70-71 0.0 4.50862929103157E-5 0.0 0.5925015182809137 4.50862929103157E-5 72-73 0.0 4.50862929103157E-5 0.0 0.6686748101528921 4.50862929103157E-5 74-75 0.0 4.50862929103157E-5 0.0 0.7434278837981956 4.50862929103157E-5 76-77 0.0 4.50862929103157E-5 0.0 0.8252595054304186 4.50862929103157E-5 78-79 0.0 9.01725858206314E-5 0.0 0.9259822837920638 4.50862929103157E-5 80-81 0.0 9.01725858206314E-5 0.0 1.0400731480016177 4.50862929103157E-5 82-83 0.0 9.01725858206314E-5 0.0 1.1570269918109766 4.50862929103157E-5 84-85 0.0 9.01725858206314E-5 0.0 1.2712305717528063 4.50862929103157E-5 86-87 0.0 9.01725858206314E-5 0.0 1.3946317554483403 4.50862929103157E-5 88-89 0.0 9.01725858206314E-5 0.0 1.5370593547520277 4.50862929103157E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2060 0.0 58.96929 1 GTATCAA 4130 0.0 49.022083 1 ATCAACG 5090 0.0 38.80924 3 TCAACGC 5245 0.0 37.659798 4 CAACGCA 5320 0.0 37.395325 5 TATCAAC 5330 0.0 36.445324 2 AACGCAG 5675 0.0 35.054497 6 ACGCAGA 6695 0.0 30.086592 7 CGCAGAG 6800 0.0 29.634884 8 GCAGAGT 7220 0.0 26.967983 9 GTGGTAT 1010 0.0 24.866774 1 GTGTAGC 775 0.0 23.80924 1 TGGTATC 990 0.0 21.744223 2 GAGTACT 4835 0.0 21.497536 12-13 AGAGTAC 6565 0.0 21.386604 10-11 CAGAGTA 7010 0.0 21.303242 10-11 GTACATG 4165 0.0 21.290009 1 TACATGG 4260 0.0 20.573885 2 AGTACTT 5160 0.0 20.097002 12-13 ACATGGG 4325 0.0 19.807499 3 >>END_MODULE