FastQCFastQC Report
Sat 18 Jun 2016
SRR1931049_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931049_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2217969
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75190.33900383639266374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67290.3033856649935143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40350.18192319189312386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36150.061.5534251
GTATCAA70150.042.971081
ATCAACG88700.033.4952163
CAACGCA90500.032.96915
TCAACGC91300.032.593424
TATCAAC92550.032.158292
AACGCAG96250.031.2945186
ACGCAGA108350.027.4056857
CGCAGAG109250.027.1365018
GTGGTAT16250.024.8702551
GTACATG66350.024.4358881
ACATGGG62850.024.2031023
TACATGG66200.023.6283252
GCAGAGT122350.023.5331469
TGGTATC16450.023.4106862
GAGTACT91300.021.4832512-13
AGAGTAC116250.020.8304210-11
CAGAGTA119800.020.68829710-11
CATGGGG35750.020.2112834
GTACTTT98050.019.85915814-15
Produced by FastQC (version 0.11.5)