##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931049_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2217969 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.330319765515206 31.0 31.0 34.0 30.0 34.0 2 31.484556817520893 31.0 31.0 34.0 30.0 34.0 3 31.548241657119643 31.0 31.0 34.0 30.0 34.0 4 35.32613395408141 37.0 35.0 37.0 33.0 37.0 5 35.20252943120485 37.0 35.0 37.0 33.0 37.0 6 35.23250595477214 37.0 35.0 37.0 32.0 37.0 7 35.11587537968295 37.0 35.0 37.0 32.0 37.0 8 35.120123410200954 37.0 35.0 37.0 32.0 37.0 9 36.615621769285326 39.0 35.0 39.0 32.0 39.0 10-11 36.56121659049337 38.0 35.0 39.0 32.0 39.0 12-13 36.4444205487092 38.0 35.0 39.0 32.0 39.0 14-15 37.486959240638626 39.0 36.0 41.0 32.0 41.0 16-17 37.44537480009865 39.0 36.0 41.0 32.0 41.0 18-19 37.4990340261744 39.0 36.0 41.0 32.0 41.0 20-21 37.49271157531958 39.0 36.0 41.0 32.0 41.0 22-23 37.39419306581833 39.0 36.0 41.0 32.0 41.0 24-25 37.27076144887508 39.0 36.0 40.5 31.0 41.0 26-27 37.005216033226795 39.0 36.0 40.0 31.0 41.0 28-29 36.81406097199735 39.0 36.0 40.0 30.5 41.0 30-31 36.57797448927374 38.5 35.0 40.0 30.0 41.0 32-33 36.38280426822918 38.0 35.0 40.0 30.0 41.0 34-35 36.438765149557995 38.0 35.0 40.0 30.0 41.0 36-37 36.47920236937486 38.0 35.0 40.0 30.0 41.0 38-39 36.44604072464493 38.5 35.0 40.0 30.0 41.0 40-41 36.322036286350254 39.0 35.0 40.0 30.0 41.0 42-43 36.16806389088396 38.0 35.0 40.0 30.0 41.0 44-45 35.96440933123952 38.0 35.0 40.0 29.5 41.0 46-47 35.683893012030374 38.0 35.0 40.0 28.5 41.0 48-49 35.4293175423101 38.0 34.5 40.0 27.0 41.0 50-51 35.15497015512841 38.0 34.0 40.0 26.5 41.0 52-53 34.94910839601455 38.0 34.0 40.0 26.0 41.0 54-55 34.80388251594138 38.0 34.0 40.0 26.0 41.0 56-57 34.62663251830841 38.0 33.5 40.0 25.5 41.0 58-59 34.41930252406594 38.0 33.0 40.0 25.0 41.0 60-61 34.119828094982395 37.5 33.0 40.0 24.0 41.0 62-63 33.871255639731665 37.0 33.0 40.0 23.0 41.0 64-65 33.56552909441025 37.0 32.0 39.5 23.0 41.0 66-67 33.24978324764683 36.0 32.0 39.0 22.0 41.0 68-69 32.91129474758213 36.0 31.5 39.0 22.0 40.0 70-71 32.544450125317354 35.5 31.0 39.0 21.5 40.0 72-73 32.123578598258135 35.0 31.0 38.0 20.5 40.0 74-75 31.619572230270126 35.0 30.5 37.0 19.0 39.5 76-77 30.23743974780531 33.5 28.5 36.0 18.0 38.5 78-79 30.968096262842266 34.0 30.0 36.0 18.5 39.0 80-81 30.93455679497775 34.0 30.0 36.0 18.5 38.5 82-83 30.66248919619706 34.0 30.0 36.0 18.0 37.0 84-85 30.316217900250184 34.0 30.0 35.0 17.5 37.0 86-87 29.963898503540854 34.0 30.0 35.0 12.0 36.5 88-89 29.61172022692833 34.0 29.0 35.0 7.5 36.0 90-91 29.27061897618948 34.0 29.0 35.0 4.5 36.0 92-93 28.94965348929584 34.0 29.0 35.0 2.0 35.5 94-95 28.562427157458018 34.0 29.0 35.0 2.0 35.0 96-97 28.141671051308652 34.0 29.0 35.0 2.0 35.0 98-99 27.648193459872523 33.5 28.0 35.0 2.0 35.0 100-101 26.522593643103217 32.5 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3163976257752168 1101 2 0.35622731201506497 1101 3 0.2885838807160823 1101 4 0.26060134604379925 1101 5 0.20078716051304468 1101 6 0.24475649300706692 1101 7 0.3714686794983919 1101 8 0.36356318080695615 1101 9 0.5230302123071482 1101 10-11 0.47166570582902523 1101 12-13 0.4698479909533546 1101 14-15 0.4333754961669811 1101 16-17 0.508182392826356 1101 18-19 0.49733004666128977 1101 20-21 0.4711024789441325 1101 22-23 0.4073799790894057 1101 24-25 0.4405499304230531 1101 26-27 0.48210569366555234 1101 28-29 0.44230042101291644 1101 30-31 0.5393980072247899 1101 32-33 0.47129341974338246 1101 34-35 0.46537954829388894 1101 36-37 0.5285246384155258 1101 38-39 0.3633805930498184 1101 40-41 0.40715976966517786 1101 42-43 0.3615305927481316 1101 44-45 0.287215862900986 1101 46-47 0.23276310081506324 1101 48-49 0.24179853852540134 1101 50-51 0.267756435524781 1101 52-53 0.23678377760196412 1101 54-55 0.22480728106711467 1101 56-57 0.27736098978359536 1101 58-59 0.21210348059558726 1101 60-61 0.14942829432310134 1101 62-63 0.012380002985700855 1101 64-65 0.04383145673840971 1101 66-67 0.010063390901564162 1101 68-69 -0.004751744216761722 1101 70-71 -0.01264399142302608 1101 72-73 0.10412415692847077 1101 74-75 0.04157695623793245 1101 76-77 0.038239066459194504 1101 78-79 0.08012225019384189 1101 80-81 0.06971854891598284 1101 82-83 0.1380478285891975 1101 84-85 0.29224046893455835 1101 86-87 0.2530132665320046 1101 88-89 0.21077270184951047 1101 90-91 0.23862632395852756 1101 92-93 0.10830624257102883 1101 94-95 0.3936188557528979 1101 96-97 0.5219848577081656 1101 98-99 0.5129833227113068 1101 100-101 0.7460746182148519 1102 1 0.3725909867033437 1102 2 0.5251285116484006 1102 3 0.4134723343638491 1102 4 0.26005552331471904 1102 5 0.3196928201026168 1102 6 0.41528908769574 1102 7 0.5289757347695883 1102 8 0.42624302616983556 1102 9 0.5753944635753925 1102 10-11 0.5576404719660033 1102 12-13 0.5790794361120248 1102 14-15 0.6723129068492426 1102 16-17 0.7338704122451958 1102 18-19 0.6867148763573852 1102 20-21 0.5982698753355535 1102 22-23 0.6399754268974718 1102 24-25 0.7339974006789447 1102 26-27 0.6298752410843775 1102 28-29 0.7359697503683549 1102 30-31 0.7582216795337757 1102 32-33 0.8288814184756177 1102 34-35 0.7242221232258927 1102 36-37 0.7962607456518498 1102 38-39 0.7582714759503446 1102 40-41 0.6760025154040221 1102 42-43 0.6148571507436955 1102 44-45 0.785311461588428 1102 46-47 0.7450936462012905 1102 48-49 0.645279866491606 1102 50-51 0.5683858587100943 1102 52-53 0.5752282207811703 1102 54-55 0.6967137320616885 1102 56-57 0.5732404646854263 1102 58-59 0.5451991077508325 1102 60-61 0.5945852563147866 1102 62-63 0.4015229512255374 1102 64-65 0.44816586995050756 1102 66-67 0.2718613985347389 1102 68-69 0.40820803456941235 1102 70-71 0.4895352191077862 1102 72-73 0.4582703030518118 1102 74-75 0.5684899453385555 1102 76-77 0.502441844537465 1102 78-79 0.6525459612190438 1102 80-81 0.5950653342364625 1102 82-83 0.4744036837525947 1102 84-85 0.6496565221200115 1102 86-87 0.6761969730782731 1102 88-89 0.670119916084932 1102 90-91 0.8095385842833274 1102 92-93 0.6478313236568312 1102 94-95 0.8211109749153351 1102 96-97 0.8472781235538207 1102 98-99 0.8700269221047492 1102 100-101 1.0452515190576896 1103 1 0.3384941572017013 1103 2 0.4375016964504681 1103 3 0.4735981312511335 1103 4 0.29288017495775165 1103 5 0.381284984320601 1103 6 0.443529920531752 1103 7 0.3645474569933853 1103 8 0.33825814453069114 1103 9 0.4046848668264076 1103 10-11 0.41828016050503436 1103 12-13 0.4639217637333459 1103 14-15 0.45108894481565187 1103 16-17 0.5870982201776016 1103 18-19 0.5280237133992642 1103 20-21 0.5668623332059113 1103 22-23 0.4226698295043505 1103 24-25 0.46978036315774574 1103 26-27 0.5701520321270337 1103 28-29 0.6836948917477628 1103 30-31 0.5663795318929417 1103 32-33 0.7212687175368799 1103 34-35 0.7452362659030598 1103 36-37 0.6714414365874717 1103 38-39 0.6670369992672889 1103 40-41 0.754531790651022 1103 42-43 0.7210603202947894 1103 44-45 0.6676037498982197 1103 46-47 0.7504788518053118 1103 48-49 0.7960755696943096 1103 50-51 0.5579294660874083 1103 52-53 0.8223376394441999 1103 54-55 0.7524148507824933 1103 56-57 0.683101974437534 1103 58-59 0.7353631741731093 1103 60-61 0.636770787778218 1103 62-63 0.46869451051964006 1103 64-65 0.44789921465102367 1103 66-67 0.47908193146642475 1103 68-69 0.4009865543719968 1103 70-71 0.46572522166540153 1103 72-73 0.5326808669590974 1103 74-75 0.6945487515942972 1103 76-77 0.6689375297421236 1103 78-79 0.8405341582882038 1103 80-81 0.7242979542787076 1103 82-83 0.797580434062823 1103 84-85 0.71751058636357 1103 86-87 0.7809423006901177 1103 88-89 0.7584353934594894 1103 90-91 0.7450207898968237 1103 92-93 0.6918829685837515 1103 94-95 0.6911745364449828 1103 96-97 0.8703468855722569 1103 98-99 0.9261363123498079 1103 100-101 1.2371542917044422 1104 1 0.35022263775271867 1104 2 0.39540085533846536 1104 3 0.31004200941453774 1104 4 0.09692877664367217 1104 5 0.2713504451779585 1104 6 0.2603216922220142 1104 7 0.22067391001206715 1104 8 0.24856753710194113 1104 9 0.515719960862917 1104 10-11 0.4253839099626049 1104 12-13 0.5347016619874125 1104 14-15 0.5715923194197927 1104 16-17 0.5320977123712325 1104 18-19 0.5325639639786175 1104 20-21 0.5984972850566308 1104 22-23 0.4948715855911061 1104 24-25 0.3602700731541475 1104 26-27 0.5277326579291355 1104 28-29 0.6060859211174332 1104 30-31 0.4922564211634537 1104 32-33 0.49362743195581515 1104 34-35 0.5350062757822016 1104 36-37 0.5071928515206707 1104 38-39 0.560435978114846 1104 40-41 0.46125376204766866 1104 42-43 0.4687313356305367 1104 44-45 0.5662857615500698 1104 46-47 0.2971896514618493 1104 48-49 0.14850196085293987 1104 50-51 0.09777103050033276 1104 52-53 0.05541891616174155 1104 54-55 0.1802306370975728 1104 56-57 0.4190292750311997 1104 58-59 0.30627094730368754 1104 60-61 0.13591628815214563 1104 62-63 0.14892006887582454 1104 64-65 0.15393056938609817 1104 66-67 0.1304700894219053 1104 68-69 0.06766060731312962 1104 70-71 0.10133860559471941 1104 72-73 0.02157514167311092 1104 74-75 0.14106104512485018 1104 76-77 0.11967292569259769 1104 78-79 0.24042474909782996 1104 80-81 0.2719380395901787 1104 82-83 0.14942423282377248 1104 84-85 0.21387099962563028 1104 86-87 0.1577904850515317 1104 88-89 0.3020093346832091 1104 90-91 0.2546506452181738 1104 92-93 0.4428611883069493 1104 94-95 0.4612255017387241 1104 96-97 0.44963715692551176 1104 98-99 0.5216708477993066 1104 100-101 0.8268148923932124 1105 1 0.3090750154950648 1105 2 0.23793877830481946 1105 3 0.22860848026922653 1105 4 0.02828473000546694 1105 5 0.19901093788227087 1105 6 0.2805520298817541 1105 7 0.20216664642203597 1105 8 0.25379239987305624 1105 9 0.3569667374553589 1105 10-11 0.3128737002761568 1105 12-13 0.4137383669247967 1105 14-15 0.3653698499061875 1105 16-17 0.36568731329286663 1105 18-19 0.37470509127067686 1105 20-21 0.24693275092150202 1105 22-23 0.20447118754708526 1105 24-25 0.3538826436411071 1105 26-27 0.3694472075794195 1105 28-29 0.2755993515364139 1105 30-31 0.24718399926532442 1105 32-33 0.28901871707445537 1105 34-35 0.2056765662188056 1105 36-37 0.39850721712909376 1105 38-39 0.4820054342996869 1105 40-41 0.17193478825937092 1105 42-43 0.18339886543925132 1105 44-45 0.23109132275290278 1105 46-47 0.23095009744235995 1105 48-49 0.24364304177785812 1105 50-51 0.17945940055286513 1105 52-53 0.18064459927446563 1105 54-55 0.27923249534968164 1105 56-57 0.37610810986076615 1105 58-59 0.46676472162414484 1105 60-61 0.43636783271274737 1105 62-63 0.08990380114484964 1105 64-65 0.04515862078941524 1105 66-67 0.25252176381048486 1105 68-69 0.12669595690348956 1105 70-71 0.09008851196877288 1105 72-73 0.24596978305898887 1105 74-75 0.4117108270209826 1105 76-77 0.455125058759144 1105 78-79 0.28104182433029123 1105 80-81 0.348055933380909 1105 82-83 0.42098530725729333 1105 84-85 0.345292936987196 1105 86-87 0.47068197525558375 1105 88-89 0.5727006686656821 1105 90-91 0.46257657193669743 1105 92-93 0.37137572797046303 1105 94-95 0.4300994223653234 1105 96-97 0.2880578566412453 1105 98-99 0.501797715414007 1105 100-101 0.6037404660081727 1106 1 0.2171595963882318 1106 2 0.06614331080273317 1106 3 0.16423242698440887 1106 4 0.16161597836354957 1106 5 0.13236774207985746 1106 6 0.13826301299524602 1106 7 0.2602150381019115 1106 8 0.1858624764869603 1106 9 0.2618034034727046 1106 10-11 0.2599509521834662 1106 12-13 0.22169999384886552 1106 14-15 0.35789128196250175 1106 16-17 0.3351489786400421 1106 18-19 0.35561148362607753 1106 20-21 0.24046256636545138 1106 22-23 0.18293324427389734 1106 24-25 0.1962041861415429 1106 26-27 0.238935947819499 1106 28-29 0.3460271348443342 1106 30-31 0.38282653204560546 1106 32-33 0.3148725591179229 1106 34-35 0.40107705124361814 1106 36-37 0.3937942199295108 1106 38-39 0.37012590059546113 1106 40-41 0.32920899884306465 1106 42-43 0.1843779400319221 1106 44-45 0.2886588893544584 1106 46-47 0.28417549458546887 1106 48-49 0.21029043731612518 1106 50-51 0.11615088438210108 1106 52-53 0.10414194271815091 1106 54-55 0.24542006448894682 1106 56-57 0.3693191921595087 1106 58-59 0.41807235853106306 1106 60-61 0.39958877992296493 1106 62-63 0.2194631505610829 1106 64-65 0.26209418969278175 1106 66-67 0.11930426574504338 1106 68-69 0.08990037323849265 1106 70-71 0.14699520098637464 1106 72-73 0.18627795431527971 1106 74-75 0.4083218188913662 1106 76-77 0.4000004874449452 1106 78-79 0.43566787806188856 1106 80-81 0.32108969747012495 1106 82-83 0.23410367368514073 1106 84-85 0.38974008197101284 1106 86-87 0.3435733951058566 1106 88-89 0.1877681593145688 1106 90-91 0.2582197480807693 1106 92-93 0.2461198568290861 1106 94-95 0.3518469056512643 1106 96-97 0.20894126661231027 1106 98-99 0.25279144440415635 1106 100-101 0.35091949449310533 1107 1 0.12818800365544192 1107 2 0.2832783918810904 1107 3 0.19817525168910066 1107 4 0.06583803022646606 1107 5 0.16943291689415219 1107 6 0.1052438422678037 1107 7 0.2698742340118727 1107 8 0.11885167222892079 1107 9 0.13970706439991432 1107 10-11 0.20248368813920337 1107 12-13 0.25094824066589894 1107 14-15 0.3068881810104642 1107 16-17 0.2588613142749949 1107 18-19 0.25651586165398044 1107 20-21 0.26117351289414614 1107 22-23 0.2602766647645822 1107 24-25 0.20028113785351565 1107 26-27 0.22953848039630032 1107 28-29 0.23417622812313255 1107 30-31 0.4109795866204138 1107 32-33 0.36885627005649724 1107 34-35 0.2780087798983786 1107 36-37 0.31722572797191617 1107 38-39 0.44514948710689595 1107 40-41 0.32570404766514827 1107 42-43 0.40950184105965093 1107 44-45 0.36798702525521065 1107 46-47 0.3963822989955901 1107 48-49 0.2881736728107569 1107 50-51 0.20564761410233956 1107 52-53 0.11549176317770815 1107 54-55 0.29038263112581575 1107 56-57 0.4477914051711238 1107 58-59 0.33833978138084575 1107 60-61 0.3577653635350515 1107 62-63 0.08919425106747525 1107 64-65 0.023905792930079883 1107 66-67 0.13917361371395032 1107 68-69 0.18651983151357854 1107 70-71 0.20192122289905967 1107 72-73 0.19422548129055883 1107 74-75 0.3635615613010188 1107 76-77 0.3251544518799321 1107 78-79 0.4097472083199136 1107 80-81 0.4055125198404852 1107 82-83 0.42595251133655054 1107 84-85 0.48145460692938613 1107 86-87 0.4509391067288142 1107 88-89 0.5242417039582854 1107 90-91 0.5464733559106953 1107 92-93 0.4687213495656799 1107 94-95 0.5809484607252173 1107 96-97 0.7434189547009247 1107 98-99 0.7261790510297779 1107 100-101 0.8762373044976748 1108 1 0.06576786702965975 1108 2 0.11688999420100643 1108 3 -0.05488443792762254 1108 4 -0.04603643133311408 1108 5 -0.08141742726130019 1108 6 0.06155289783564655 1108 7 -0.10655761704673239 1108 8 0.06377489572347628 1108 9 0.19682004510980278 1108 10-11 0.0341934501723955 1108 12-13 0.08804866557099444 1108 14-15 0.020998440654068418 1108 16-17 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-1.0350097677239027 2316 92-93 -0.867292199067375 2316 94-95 -1.3552226555306035 2316 96-97 -1.2157841497750432 2316 98-99 -1.3621263094245464 2316 100-101 -1.8847583274013928 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 30.0 5 53.0 6 114.0 7 328.0 8 831.0 9 1583.0 10 2931.0 11 4688.0 12 6569.0 13 8749.0 14 10919.0 15 13913.0 16 16570.0 17 19299.0 18 16435.0 19 11997.0 20 11203.0 21 11561.0 22 12858.0 23 14919.0 24 18324.0 25 23217.0 26 29973.0 27 38190.0 28 48180.0 29 60662.0 30 74429.0 31 90061.0 32 108714.0 33 131922.0 34 163940.0 35 204685.0 36 267907.0 37 367219.0 38 355646.0 39 69328.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.22173446871289 21.999316463754994 15.131129845735428 22.64781922179669 2 14.886127430867107 21.959573096224688 41.077855381160866 22.076444091747337 3 18.291494715560972 26.818195549267404 31.426011766843544 23.464297968328083 4 12.092110492845787 18.44630726983668 39.89499024425495 29.56659199306258 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31.445288239552145 20.21688442351526 26-27 20.17409684415936 28.327449774077184 31.233933306836843 20.264520074926615 28-29 20.078103090849904 28.367657574552542 31.37038620069018 20.183853133907373 30-31 20.38545066449276 28.15549236536156 31.2936647910256 20.16539217912008 32-33 20.286130394088257 28.37114380229318 31.05679834208612 20.285927461532445 34-35 20.42341054765646 28.271440350261322 31.228974163515254 20.07617493856696 36-37 20.455159761518143 28.19583339814554 31.21574393250694 20.133262907829383 38-39 20.222794318215122 28.39206209295458 31.22790485873312 20.157238730097184 40-41 20.38589117515378 28.352865801506 31.1109092212517 20.150333802088525 42-43 20.364826205007997 28.458612891160197 30.75284521648085 20.423715687350956 44-45 20.292092249387622 28.749240517316206 30.685106925016655 20.273560308279517 46-47 20.466712930334403 28.62462985683965 30.31882250398269 20.58983470884326 48-49 20.51383275342237 28.823675427820078 30.11664428262199 20.545847536135557 50-51 20.545747983516716 29.06961039876163 29.78920708855362 20.595434529168035 52-53 20.601878443936133 29.11150256330974 29.673388184287468 20.61323080846665 54-55 20.586926230062634 29.073918748900823 29.770086175408217 20.569068845628323 56-57 20.59101492822656 29.202249784923158 29.639369793291763 20.567365493558516 58-59 20.518375819199562 29.096922249263947 29.840622197089505 20.544079734446992 60-61 20.53826949047069 29.249141665630262 29.7999292611948 20.412659582704247 62-63 20.45419673015053 29.177945769463616 29.79052562144382 20.577331878942037 64-65 20.258450126361783 29.015661719690854 29.875911668108852 20.84997648583851 66-67 20.308536627536814 29.17146099479286 29.700085817625904 20.819916560044422 68-69 20.434274003450614 29.100769811771055 29.74661888057047 20.71833730420786 70-71 20.474057817319256 28.86423308972556 29.73251654187992 20.929192551075264 72-73 20.522881543273254 29.101933268948994 29.610512365835532 20.76467282194222 74-75 20.39683649998994 29.29058107717478 29.435136745910867 20.87744567692441 76-77 20.398053552821764 29.251393054166087 29.462319152555043 20.888234240457106 78-79 20.292956738009483 29.259985860090648 29.47512800766152 20.971929394238348 80-81 20.404469407238835 29.232822392573382 29.37385241217466 20.988855788013126 82-83 20.499339711240328 29.117156281264528 29.53548945003289 20.848014557462253 84-85 20.58890363210667 29.14420805701072 29.28471948886571 20.9821688220169 86-87 20.281753261655144 29.282825864563485 29.38510412003053 21.05031675375084 88-89 20.42441137510674 29.166046729300632 29.264470239850386 21.145071655742242 90-91 20.382236398330612 29.090037225811336 29.4870268768915 21.04069949896655 92-93 20.413125050449572 29.144993080235448 29.324559730262212 21.117322139052767 94-95 20.124910923641526 29.03227624503444 29.46272837758051 21.380084453743528 96-97 20.156751514561293 28.917762150868654 29.515786740031082 21.409699594538967 98-99 20.059725812218296 29.05078925810054 29.39008615539712 21.49939877428404 100-101 20.138559195371982 28.99177130068094 29.243262642534678 21.626406861412402 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 341.0 1 242.5 2 185.0 3 250.5 4 225.0 5 238.0 6 457.5 7 940.0 8 1523.0 9 1920.0 10 1844.0 11 1614.0 12 1698.5 13 1959.5 14 2257.0 15 2567.0 16 2915.5 17 3255.5 18 3650.0 19 4109.5 20 4723.5 21 5680.5 22 6792.0 23 8446.0 24 10820.5 25 13604.0 26 17720.0 27 22605.5 28 27253.5 29 33191.0 30 39822.5 31 46153.5 32 53573.5 33 60325.0 34 66171.0 35 71716.5 36 78887.0 37 86321.5 38 89283.0 39 89547.0 40 88831.0 41 87446.0 42 86691.0 43 87960.0 44 89569.0 45 90680.0 46 89355.5 47 86918.0 48 85082.0 49 81591.0 50 76309.0 51 69702.0 52 62902.0 53 56118.5 54 49091.0 55 40825.5 56 32567.0 57 25409.5 58 19739.5 59 15187.0 60 10922.0 61 7698.5 62 5303.5 63 3287.0 64 1817.0 65 1001.5 66 557.5 67 305.0 68 165.0 69 98.0 70 58.5 71 29.5 72 21.5 73 18.5 74 13.0 75 9.0 76 6.0 77 4.0 78 5.0 79 5.0 80 4.5 81 3.5 82 1.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004102852654838728 2 0.006266994714533882 3 0.0018936243022332595 4 0.17534059312821773 5 0.001487847666040418 6 5.410355149237884E-4 7 4.50862929103157E-5 8 5.861218078341041E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.5780202518610496E-4 24-25 6.08664954289262E-4 26-27 0.002953152185625678 28-29 1.352588787309471E-4 30-31 0.006740400790092198 32-33 0.02179922262213764 34-35 0.013999293948653024 36-37 0.0010144415904821031 38-39 1.803451716412628E-4 40-41 0.0017132791305919965 42-43 0.01151954783858566 44-45 0.007957730698670722 46-47 0.011091228055937662 48-49 0.010978512323661873 50-51 0.1844029380031912 52-53 0.11593940221887682 54-55 0.017989430871215965 56-57 0.0070785479869195644 58-59 0.018395207507408806 60-61 0.12522717855840185 62-63 0.241639986852837 64-65 0.24673473795170267 66-67 0.25787105230055063 68-69 0.2529341032268711 70-71 0.2401972254797069 72-73 0.16512854778403124 74-75 0.2557519965337658 76-77 0.1982669730731133 78-79 0.005996476957071987 80-81 6.762943936547355E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.352588787309471E-4 90-91 0.0068982028152783014 92-93 0.018395207507408806 94-95 0.003088411064356625 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2217969.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.78887863370843 #Duplication Level Percentage of deduplicated Percentage of total 1 73.97499604884327 41.26982076497977 2 15.161835919531564 16.917236479578946 3 4.830171800220488 8.084096050273853 4 2.1331310723308174 4.760199620162251 5 1.0617695070700162 2.9617465083450774 6 0.6700007501478935 2.2427154320696663 7 0.42428429341197693 1.656924146594466 8 0.2993888179816064 1.3362053144532209 9 0.21157415670412555 1.0623136455356101 >10 1.109762420120886 11.19751627728509 >50 0.08281555024223598 3.18903189968253 >100 0.03865069774106257 3.883030545224865 >500 0.0011332759578454817 0.43484698029309143 >1k 3.2379313081291183E-4 0.3608746722942599 >5k 1.6189656540645592E-4 0.6434416632272075 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7519 0.33900383639266374 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6729 0.3033856649935143 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4035 0.18192319189312386 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 4.50862929103157E-5 0.0 7 0.0 0.0 0.0 4.50862929103157E-5 0.0 8 0.0 0.0 0.0 4.50862929103157E-5 0.0 9 0.0 0.0 0.0 4.50862929103157E-5 0.0 10-11 0.0 0.0 0.0 4.50862929103157E-5 0.0 12-13 0.0 0.0 0.0 6.762943936547355E-5 0.0 14-15 0.0 0.0 0.0 9.01725858206314E-5 0.0 16-17 0.0 0.0 0.0 9.01725858206314E-5 0.0 18-19 0.0 0.0 0.0 9.01725858206314E-5 0.0 20-21 0.0 0.0 0.0 9.01725858206314E-5 0.0 22-23 0.0 0.0 0.0 1.352588787309471E-4 0.0 24-25 0.0 0.0 0.0 2.254314645515785E-4 0.0 26-27 0.0 0.0 0.0 3.606903432825256E-4 0.0 28-29 0.0 0.0 0.0 9.918984440269454E-4 0.0 30-31 0.0 0.0 0.0 0.002975695332080836 0.0 32-33 0.0 0.0 0.0 0.0077999286734846155 0.0 34-35 0.0 0.0 0.0 0.016433953765810073 0.0 36-37 0.0 0.0 0.0 0.0297344101743532 0.0 38-39 0.0 0.0 0.0 0.05094751098865674 0.0 40-41 0.0 0.0 0.0 0.07601548984679227 0.0 42-43 0.0 0.0 0.0 0.10239097119932694 0.0 44-45 0.0 0.0 0.0 0.1336583153326309 0.0 46-47 0.0 0.0 0.0 0.17383020231572216 0.0 48-49 0.0 0.0 0.0 0.2186910637614863 0.0 50-51 0.0 0.0 0.0 0.26914262552812956 0.0 52-53 0.0 0.0 0.0 0.31691155286660905 0.0 54-55 0.0 0.0 0.0 0.37184920077782874 0.0 56-57 0.0 0.0 0.0 0.4394786401433023 0.0 58-59 0.0 2.254314645515785E-5 0.0 0.5154039574042739 0.0 60-61 0.0 4.50862929103157E-5 0.0 0.5993095485103714 0.0 62-63 0.0 4.50862929103157E-5 0.0 0.6882648044224242 0.0 64-65 0.0 4.50862929103157E-5 0.0 0.7813454561357711 0.0 66-67 0.0 4.50862929103157E-5 0.0 0.8840520313854703 0.0 68-69 0.0 4.50862929103157E-5 0.0 1.0069347227125356 0.0 70-71 0.0 4.50862929103157E-5 0.0 1.1451016673361982 0.0 72-73 0.0 4.50862929103157E-5 0.0 1.2910234543404349 0.0 74-75 0.0 4.50862929103157E-5 0.0 1.4383429164248915 0.0 76-77 0.0 4.50862929103157E-5 0.0 1.5980385659132297 0.0 78-79 0.0 4.50862929103157E-5 0.0 1.788167463116031 0.0 80-81 0.0 4.50862929103157E-5 0.0 2.0076475370034474 0.0 82-83 0.0 4.50862929103157E-5 0.0 2.2391205648050088 0.0 84-85 0.0 4.50862929103157E-5 0.0 2.4693086332586254 0.0 86-87 0.0 4.50862929103157E-5 0.0 2.7197404472289737 0.0 88-89 0.0 4.50862929103157E-5 0.0 3.004280041785976 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3615 0.0 61.553425 1 GTATCAA 7015 0.0 42.97108 1 ATCAACG 8870 0.0 33.495216 3 CAACGCA 9050 0.0 32.9691 5 TCAACGC 9130 0.0 32.59342 4 TATCAAC 9255 0.0 32.15829 2 AACGCAG 9625 0.0 31.294518 6 ACGCAGA 10835 0.0 27.405685 7 CGCAGAG 10925 0.0 27.136501 8 GTGGTAT 1625 0.0 24.870255 1 GTACATG 6635 0.0 24.435888 1 ACATGGG 6285 0.0 24.203102 3 TACATGG 6620 0.0 23.628325 2 GCAGAGT 12235 0.0 23.533146 9 TGGTATC 1645 0.0 23.410686 2 GAGTACT 9130 0.0 21.48325 12-13 AGAGTAC 11625 0.0 20.83042 10-11 CAGAGTA 11980 0.0 20.688297 10-11 CATGGGG 3575 0.0 20.211283 4 GTACTTT 9805 0.0 19.859158 14-15 >>END_MODULE