FastQCFastQC Report
Sat 18 Jun 2016
SRR1931048_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931048_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2815349
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1254394.455539970355363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20700.057.920971
GTATCAA41100.048.744461
ATCAACG50650.038.2894133
TCAACGC51700.037.701624
TATCAAC53600.035.9341472
CAACGCA53400.035.8354155
AACGCAG57600.033.1411136
ACGCAGA64500.028.8908187
CGCAGAG66450.027.9073568
TACATGG46750.024.2155882
GCAGAGT71950.023.9533219
GTGTAGC11200.023.3314911
GTACATG50600.022.5810781
ACATGGG48550.022.5147933
AGAGTAC63950.021.34309210-11
AACGCCG2501.1305019E-820.5864776
GAGTACT48000.020.40908612-13
CAGAGTA69650.020.02385910-11
AGTACTT52300.019.0065512-13
GTATAAT22000.018.6319221
Produced by FastQC (version 0.11.5)