FastQCFastQC Report
Sat 18 Jun 2016
SRR1931048_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931048_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2815349
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71660.2545332745602765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60370.21443167436790253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37690.1338732782330006No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT30300.10762431229662822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37750.059.3021
GTATCAA72000.041.7205471
ATCAACG87150.033.591133
TCAACGC89350.032.710274
CAACGCA90250.032.3825
TATCAAC93450.031.5322232
AACGCAG93300.031.4754146
ACGCAGA108350.026.9278037
CGCAGAG108500.026.8031278
GGCGTGC10000.026.0926048
ACGCTCG1109.56173E-525.8769638
GCGTGCG9900.024.4384869
TACATGG70950.023.9143182
ACATGGG70700.023.1905463
GCAGAGT126500.022.4634069
GTACATG80000.022.0419161
TGGTATC17600.021.8733122
AGGCGTG14750.021.5495157
GTGGTAT18350.021.4984631
GAGTACT91850.021.30512412-13
Produced by FastQC (version 0.11.5)