##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931048_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2815349 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.35974119016861 31.0 31.0 34.0 30.0 34.0 2 31.524003596001773 31.0 31.0 34.0 30.0 34.0 3 31.593864206533542 31.0 31.0 34.0 30.0 34.0 4 35.35941476527422 37.0 35.0 37.0 33.0 37.0 5 35.23957100878079 37.0 35.0 37.0 33.0 37.0 6 35.267568248199424 37.0 35.0 37.0 32.0 37.0 7 35.15409386189776 37.0 35.0 37.0 32.0 37.0 8 35.1588129926343 37.0 35.0 37.0 32.0 37.0 9 36.66627263618116 39.0 35.0 39.0 32.0 39.0 10-11 36.610310124961416 39.0 35.0 39.0 32.0 39.0 12-13 36.5006940524958 38.0 35.0 39.0 32.0 39.0 14-15 37.56693894788887 39.0 36.0 41.0 32.0 41.0 16-17 37.52761131923609 39.0 36.0 41.0 32.0 41.0 18-19 37.558874583577385 39.0 36.0 41.0 32.0 41.0 20-21 37.547390216985534 39.0 36.0 41.0 32.0 41.0 22-23 37.452487240480664 39.0 36.0 41.0 32.0 41.0 24-25 37.33057784310222 39.0 36.0 41.0 31.5 41.0 26-27 37.085510002489926 39.0 36.0 40.0 31.0 41.0 28-29 36.9025405020834 39.0 36.0 40.0 31.0 41.0 30-31 36.68023467072821 38.5 35.5 40.0 30.0 41.0 32-33 36.51409008261498 38.0 35.0 40.0 30.0 41.0 34-35 36.57795925123315 38.5 35.0 40.0 30.0 41.0 36-37 36.64007037848593 39.0 35.0 40.0 30.0 41.0 38-39 36.61943741255524 39.0 35.0 40.0 30.0 41.0 40-41 36.505153712736856 39.0 35.0 40.0 30.0 41.0 42-43 36.367689405469804 39.0 35.0 40.0 30.0 41.0 44-45 36.19821592278613 39.0 35.0 40.0 30.0 41.0 46-47 35.97497841297829 38.5 35.0 40.0 29.0 41.0 48-49 35.761986879779386 38.0 35.0 40.0 28.0 41.0 50-51 35.51524659997748 38.0 34.5 40.0 27.5 41.0 52-53 35.322627319028655 38.0 34.0 40.0 27.0 41.0 54-55 35.18366817044708 38.0 34.0 40.0 27.0 41.0 56-57 35.02612571301107 38.0 34.0 40.0 26.0 41.0 58-59 34.83530514334102 38.0 34.0 40.0 26.0 41.0 60-61 34.549067096122 38.0 33.0 40.0 26.0 41.0 62-63 34.32242343666806 37.5 33.0 40.0 25.5 41.0 64-65 34.03257358146361 37.0 33.0 40.0 25.0 41.0 66-67 33.735237087835294 37.0 32.0 39.5 24.0 41.0 68-69 33.40239824618546 36.0 32.0 39.0 23.0 40.5 70-71 33.05233063467442 36.0 31.5 39.0 22.5 40.0 72-73 32.63890835558931 35.0 31.0 38.5 22.0 40.0 74-75 32.1355471382056 35.0 31.0 38.0 21.0 40.0 76-77 30.736605301864884 33.5 29.5 36.0 20.0 39.0 78-79 31.472187995165072 34.5 30.5 37.0 21.0 39.0 80-81 31.434954955850944 34.5 31.0 36.0 21.0 39.0 82-83 31.153937753365568 34.0 31.0 36.0 20.0 37.5 84-85 30.79668897177579 34.0 30.5 35.5 20.0 37.0 86-87 30.434263034529643 34.0 30.0 35.0 18.0 37.0 88-89 30.06743373556884 34.0 30.0 35.0 17.5 36.0 90-91 29.72095146995985 34.0 29.5 35.0 12.0 36.0 92-93 29.396615481775083 34.0 29.0 35.0 7.0 36.0 94-95 29.00542863424748 34.0 29.0 35.0 2.0 35.0 96-97 28.58233437488567 34.0 29.0 35.0 2.0 35.0 98-99 28.089123586454114 34.0 29.0 35.0 2.0 35.0 100-101 26.958793918622522 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29720914194822967 1101 2 0.40074072519394477 1101 3 0.2760419071850464 1101 4 0.18557011509599874 1101 5 0.1886106608947884 1101 6 0.2781451365755885 1101 7 0.36711252160755947 1101 8 0.33221108577672 1101 9 0.48313401107101583 1101 10-11 0.5000712169728772 1101 12-13 0.4380278154478816 1101 14-15 0.5380798279439745 1101 16-17 0.5758242293491733 1101 18-19 0.455312018141818 1101 20-21 0.5274491250036348 1101 22-23 0.536822068523044 1101 24-25 0.4638870131285273 1101 26-27 0.5332267491415124 1101 28-29 0.544300913570666 1101 30-31 0.5790835498047855 1101 32-33 0.45453022862339054 1101 34-35 0.560019733047902 1101 36-37 0.560705836061409 1101 38-39 0.6276304630239125 1101 40-41 0.6553723704151935 1101 42-43 0.623339445215926 1101 44-45 0.5653216395708753 1101 46-47 0.6071942396877148 1101 48-49 0.6149737344022483 1101 50-51 0.5394485839986132 1101 52-53 0.37874467931985123 1101 54-55 0.44530146649209 1101 56-57 0.5018971986387939 1101 58-59 0.6746972526509865 1101 60-61 0.47335382417411864 1101 62-63 0.4363742366569099 1101 64-65 0.4319322727277637 1101 66-67 0.47311490267634326 1101 68-69 0.5412370571301892 1101 70-71 0.49693106138069965 1101 72-73 0.45696786394123734 1101 74-75 0.5165625566442813 1101 76-77 0.5641046299378871 1101 78-79 0.7439645032499307 1101 80-81 0.6835150901506175 1101 82-83 0.7506276028469578 1101 84-85 0.5746926164909567 1101 86-87 0.5787134613305014 1101 88-89 0.660926283302441 1101 90-91 0.7419199201338742 1101 92-93 0.45482629143143427 1101 94-95 0.5441069498506899 1101 96-97 0.6333081447073674 1101 98-99 0.7661178357861687 1101 100-101 1.1672441409217775 1102 1 0.26435709157401277 1102 2 0.40852898567794327 1102 3 0.2748283124577391 1102 4 0.21058506136499489 1102 5 0.3224893340650752 1102 6 0.35313436135840703 1102 7 0.33062923815557355 1102 8 0.30773704634274424 1102 9 0.3706473931955827 1102 10-11 0.3760421501822506 1102 12-13 0.3683104979057106 1102 14-15 0.47233087327605006 1102 16-17 0.45966593732838845 1102 18-19 0.4788169227546959 1102 20-21 0.42345597761860887 1102 22-23 0.3662279233498893 1102 24-25 0.4363739695241122 1102 26-27 0.4728931496265645 1102 28-29 0.35858465027694564 1102 30-31 0.36077305549887484 1102 32-33 0.5282901416720733 1102 34-35 0.5096271403523147 1102 36-37 0.5688763825115117 1102 38-39 0.5268362705788334 1102 40-41 0.6163192354466034 1102 42-43 0.5504987957166634 1102 44-45 0.667044404869408 1102 46-47 0.6446461743605809 1102 48-49 0.6440184003221603 1102 50-51 0.637220879630668 1102 52-53 0.6418948269085902 1102 54-55 0.6964009013447949 1102 56-57 0.5659371101641355 1102 58-59 0.6014044369552636 1102 60-61 0.5233675765550956 1102 62-63 0.39444458718847386 1102 64-65 0.48667376121294126 1102 66-67 0.4697890026812459 1102 68-69 0.6681101163065293 1102 70-71 0.5826916345436643 1102 72-73 0.541991974434211 1102 74-75 0.37229675546079477 1102 76-77 0.35936951893048175 1102 78-79 0.5020418659845483 1102 80-81 0.3763689298456079 1102 82-83 0.20709288428112416 1102 84-85 0.367764136730532 1102 86-87 0.4598259110738603 1102 88-89 0.3920317510677691 1102 90-91 0.3826156636661757 1102 92-93 0.3905876202197298 1102 94-95 0.5403763369297359 1102 96-97 0.661665675178714 1102 98-99 0.8081781782113886 1102 100-101 1.0199512442230123 1103 1 0.35916648608482404 1103 2 0.4018914662788795 1103 3 0.463349907102522 1103 4 0.233233513373321 1103 5 0.3613494438679439 1103 6 0.3400452675062695 1103 7 0.39665888445490793 1103 8 0.38702325614109157 1103 9 0.5561227090040006 1103 10-11 0.481448678280195 1103 12-13 0.5154385424562093 1103 14-15 0.46454484584678823 1103 16-17 0.5308852951508314 1103 18-19 0.584978792598001 1103 20-21 0.5465949786174633 1103 22-23 0.4579930057497208 1103 24-25 0.5556667708324028 1103 26-27 0.6043416845949352 1103 28-29 0.647035740796376 1103 30-31 0.5670982827450572 1103 32-33 0.6245151612797315 1103 34-35 0.6597696981442525 1103 36-37 0.6372848725670295 1103 38-39 0.7073159847004007 1103 40-41 0.6721956917173628 1103 42-43 0.7051166073353627 1103 44-45 0.8325781979794371 1103 46-47 0.7599911618331348 1103 48-49 0.6905802663512048 1103 50-51 0.6555172067412016 1103 52-53 0.6256347757498943 1103 54-55 0.6066723332877473 1103 56-57 0.7149103603149172 1103 58-59 0.7698191251756441 1103 60-61 0.6054105393732598 1103 62-63 0.4810183489262485 1103 64-65 0.3929420726182755 1103 66-67 0.41274687423142353 1103 68-69 0.34917454384640934 1103 70-71 0.45383518404197076 1103 72-73 0.3834652067056723 1103 74-75 0.4723649352743209 1103 76-77 0.640873837382653 1103 78-79 0.7263059531021376 1103 80-81 0.6306669399962992 1103 82-83 0.6138783252686331 1103 84-85 0.5494932578545928 1103 86-87 0.5467400950973804 1103 88-89 0.625655961099735 1103 90-91 0.5027749565032416 1103 92-93 0.4349105183862676 1103 94-95 0.5031036725055351 1103 96-97 0.6402195479589956 1103 98-99 0.613600022928285 1103 100-101 0.8596723029649276 1104 1 0.2932867917036326 1104 2 0.4107625030016848 1104 3 0.3254512097969169 1104 4 0.22019489489979094 1104 5 0.30438875587886116 1104 6 0.34841857556401834 1104 7 0.3891207110012118 1104 8 0.4607335655743583 1104 9 0.4797244798361291 1104 10-11 0.45235923507512865 1104 12-13 0.430193608168878 1104 14-15 0.4891338093551667 1104 16-17 0.533235982330801 1104 18-19 0.555268286662475 1104 20-21 0.5536268119835412 1104 22-23 0.57968437005335 1104 24-25 0.44750019471089786 1104 26-27 0.4944453684343628 1104 28-29 0.5280829244566334 1104 30-31 0.4506954299946031 1104 32-33 0.6079030336226197 1104 34-35 0.6454665298342803 1104 36-37 0.5623637046500747 1104 38-39 0.6013287920241552 1104 40-41 0.5646127851021703 1104 42-43 0.4763237669529943 1104 44-45 0.6462698974928074 1104 46-47 0.49804902596915923 1104 48-49 0.3611126140908496 1104 50-51 0.2385875545502003 1104 52-53 0.16500654646643653 1104 54-55 0.3737153210468094 1104 56-57 0.4309832048585136 1104 58-59 0.43177334014912105 1104 60-61 0.35898006071686694 1104 62-63 0.2626592005901571 1104 64-65 0.28657938724150966 1104 66-67 0.3320825232090385 1104 68-69 0.22835391573271835 1104 70-71 0.26281863161631946 1104 72-73 0.2595787387759927 1104 74-75 0.4798428865648816 1104 76-77 0.43621731177378464 1104 78-79 0.6054163180183956 1104 80-81 0.519918439770894 1104 82-83 0.36740542524204756 1104 84-85 0.46547178131349654 1104 86-87 0.42864842101371536 1104 88-89 0.551660954568927 1104 90-91 0.43450591023274043 1104 92-93 0.3859283422971238 1104 94-95 0.6260979045862634 1104 96-97 0.6638522967944311 1104 98-99 0.7350470886871889 1104 100-101 0.9871970680291398 1105 1 0.29122563234370347 1105 2 0.2944823834521877 1105 3 0.29764931073856715 1105 4 0.21202703029916847 1105 5 0.18549532040687922 1105 6 0.16500466076836062 1105 7 0.19223608977160467 1105 8 0.3151629193654415 1105 9 0.37071414026829075 1105 10-11 0.3729587836688495 1105 12-13 0.40337534703991196 1105 14-15 0.43572062626545005 1105 16-17 0.3364779462295573 1105 18-19 0.4274335171217132 1105 20-21 0.4935426095148827 1105 22-23 0.4627164469162537 1105 24-25 0.42278519417618554 1105 26-27 0.41295381400951214 1105 28-29 0.4260792321558782 1105 30-31 0.32687384241784656 1105 32-33 0.3477892252057444 1105 34-35 0.4202836877758287 1105 36-37 0.4250183901009379 1105 38-39 0.34971020219036575 1105 40-41 0.38707351219274244 1105 42-43 0.3471656790694766 1105 44-45 0.29641770209354235 1105 46-47 0.336836476649367 1105 48-49 0.37333626845548196 1105 50-51 0.31546249474731525 1105 52-53 0.2153505608303803 1105 54-55 0.34994354575933784 1105 56-57 0.3298882350554564 1105 58-59 0.3578292199692328 1105 60-61 0.3799544678943434 1105 62-63 0.2272208243019307 1105 64-65 0.17745906664173816 1105 66-67 0.35809050443234014 1105 68-69 0.3170598656277477 1105 70-71 0.37139878437401563 1105 72-73 0.3960270381225328 1105 74-75 0.3678386314133846 1105 76-77 0.2641653831657713 1105 78-79 0.45970889908296897 1105 80-81 0.36530075612677493 1105 82-83 0.3094275227615668 1105 84-85 0.4375870306282259 1105 86-87 0.3628937226172759 1105 88-89 0.4392710340997361 1105 90-91 0.45943423119273774 1105 92-93 0.48636049908482093 1105 94-95 0.6045412846174436 1105 96-97 0.43157162565122675 1105 98-99 0.5021535293880532 1105 100-101 0.5109060291755547 1106 1 0.13938634521413107 1106 2 0.2042683229630775 1106 3 0.1888106045989204 1106 4 0.08549310699205392 1106 5 0.13767698532377182 1106 6 0.20407573401904955 1106 7 0.294484434216713 1106 8 0.19640398223290134 1106 9 0.28425020660015576 1106 10-11 0.16573286294855194 1106 12-13 0.22353643021648395 1106 14-15 0.3189721762564517 1106 16-17 0.32856792485258524 1106 18-19 0.29519614643748326 1106 20-21 0.17991782955066782 1106 22-23 0.0733812641242082 1106 24-25 0.22254591601566176 1106 26-27 0.27811009186434177 1106 28-29 0.3605327675029031 1106 30-31 0.3730682584763443 1106 32-33 0.40618570632046413 1106 34-35 0.45333917309111627 1106 36-37 0.18796256600435868 1106 38-39 0.3017238084994247 1106 40-41 0.26285139377069555 1106 42-43 0.3009848053822779 1106 44-45 0.38542488199239955 1106 46-47 0.28421565039155183 1106 48-49 0.3391309026127587 1106 50-51 0.19587625193170766 1106 52-53 0.05471467101555305 1106 54-55 0.38238540625026474 1106 56-57 0.36002101023588295 1106 58-59 0.30294337660917847 1106 60-61 0.33140728682773357 1106 62-63 0.1045130093990636 1106 64-65 0.14132679896722067 1106 66-67 0.14968317832026656 1106 68-69 0.18301358934693468 1106 70-71 0.16486624416481988 1106 72-73 0.2877450122459422 1106 74-75 0.389485889937923 1106 76-77 0.2581693872675608 1106 78-79 0.3603969181470781 1106 80-81 0.13728629642632129 1106 82-83 0.33377404229443997 1106 84-85 0.4417551949109537 1106 86-87 0.2648515054957663 1106 88-89 0.30118502405751 1106 90-91 0.23431639591192877 1106 92-93 0.2959804002432733 1106 94-95 0.40179444770467043 1106 96-97 0.2898418852052025 1106 98-99 0.3634664378653767 1106 100-101 0.4774308413528523 1107 1 0.13771198446114497 1107 2 0.21764347820520058 1107 3 0.11722413180243407 1107 4 0.045739660195337706 1107 5 0.11333438135219609 1107 6 0.15639492207202466 1107 7 0.11304611754560057 1107 8 0.16362178438033936 1107 9 0.17564140197578837 1107 10-11 0.21184854992312552 1107 12-13 0.20577668742652122 1107 14-15 0.2791469160278268 1107 16-17 0.30609555648969433 1107 18-19 0.2602787803844109 1107 20-21 0.17662267877056337 1107 22-23 0.1988149141898603 1107 24-25 0.2268009858620701 1107 26-27 0.23923235007104893 1107 28-29 0.23831490417902756 1107 30-31 0.305340641587712 1107 32-33 0.3102673767659212 1107 34-35 0.29612496547325406 1107 36-37 0.23341550814499357 1107 38-39 0.14479828757095703 1107 40-41 0.25106462157337717 1107 42-43 0.4977188870229696 1107 44-45 0.4712970470290472 1107 46-47 0.4017077159167073 1107 48-49 0.1830582144127888 1107 50-51 0.15091498103127066 1107 52-53 0.21653695310863696 1107 54-55 0.26483480974685136 1107 56-57 0.3647490677051408 1107 58-59 0.2696060162857492 1107 60-61 0.17151283438503384 1107 62-63 0.14048343703564825 1107 64-65 0.1406875326613033 1107 66-67 0.11426091368727498 1107 68-69 0.008277455484495988 1107 70-71 0.08538569502299964 1107 72-73 0.0790577694970338 1107 74-75 0.3011770284690627 1107 76-77 0.2591031272240123 1107 78-79 0.40781268727443987 1107 80-81 0.30271225699848614 1107 82-83 0.45824182099090294 1107 84-85 0.28820529107922255 1107 86-87 0.27211992867231416 1107 88-89 0.23034760448662794 1107 90-91 0.17625851549832205 1107 92-93 0.1624344778408009 1107 94-95 0.3559017516322349 1107 96-97 0.49988994564179734 1107 98-99 0.5149108798970552 1107 100-101 0.8216874587980172 1108 1 0.08498239209947656 1108 2 0.19160731198315872 1108 3 0.08785033311890089 1108 4 0.051197539470599907 1108 5 0.15836293636941434 1108 6 0.14921418322823854 1108 7 0.13675205894956832 1108 8 0.1926668977894792 1108 9 0.3026649294690813 1108 10-11 0.18434126541222184 1108 12-13 0.21665223153838298 1108 14-15 0.09645720178598793 1108 16-17 0.21737968372352867 1108 18-19 0.199339136559324 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-1.5610047357948105 2316 90-91 -1.531355971016108 2316 92-93 -1.450364747931669 2316 94-95 -1.920260894407786 2316 96-97 -1.7620752165466733 2316 98-99 -1.8274739963891768 2316 100-101 -2.10932964484579 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 13.0 4 28.0 5 51.0 6 91.0 7 299.0 8 845.0 9 1750.0 10 3129.0 11 5235.0 12 7452.0 13 9748.0 14 12176.0 15 14802.0 16 17666.0 17 20103.0 18 17168.0 19 13460.0 20 12674.0 21 13455.0 22 14961.0 23 17502.0 24 21626.0 25 27681.0 26 35735.0 27 46788.0 28 59376.0 29 74798.0 30 92431.0 31 111889.0 32 135323.0 33 165296.0 34 205071.0 35 256839.0 36 337031.0 37 469568.0 38 487515.0 39 105733.0 40 37.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.357494769521495 22.476636011977707 15.283049697538035 22.882819520962762 2 14.313380857046642 22.211664291428253 41.711152064041926 21.76380278748318 3 17.91908869554098 27.138480555392757 31.678201828931503 23.264228920134762 4 11.708128314123883 18.635841408086577 40.91746370497301 28.738566572816527 5 11.346874085356323 39.373225768869005 37.1625586437557 12.117341502018965 6 27.964176097483524 41.36251312902551 18.205025561861905 12.468285211629057 7 24.437245013579147 34.07669963123538 24.09824582839907 17.387809526786405 8 23.327080668610073 38.657309810242694 21.075410972698432 16.940198548448798 9 24.266867091788622 16.899006126771493 22.237491692859393 36.59663508858049 10-11 22.866649925106977 28.38328391968456 30.03663133771337 18.713434817495095 12-13 23.38390373626858 25.659767226017095 30.37119021478332 20.58513882293101 14-15 20.582741251617474 27.467962231325494 28.105218926676585 23.844077590380447 16-17 19.751334559232266 30.68214633425554 28.887786203415633 20.67873290309656 18-19 19.653602448577423 29.459242886050717 31.196363221753327 19.690791443618537 20-21 20.51019251964854 28.728711786709216 31.182475067922304 19.578620625719935 22-23 20.01348326420023 28.49499279306031 31.13307370827416 20.358450234465298 24-25 19.887924587345065 28.64119440074037 31.399461485332214 20.071419526582346 26-27 19.94384222542498 28.841244173542087 31.084358319280568 20.130555281752365 28-29 19.880629131045644 28.94504702534522 31.17324759838364 20.001076245225494 30-31 20.299358390870946 28.68226361476811 31.074875119330418 19.94350287503053 32-33 20.16807260065607 28.92227872326782 30.88972182881492 20.019926847261196 34-35 20.103602297055183 28.87667138366651 31.05646172436155 19.96326459491676 36-37 20.168474511974118 28.68084006021336 31.155157385194304 19.995528042618215 38-39 19.996021794880903 29.01463055971925 31.115282701201274 19.87406494419857 40-41 20.232315817945867 29.03194697697994 30.89835188401401 19.837385321060182 42-43 20.2377459217015 28.922136310124753 30.74338317782241 20.096734590351335 44-45 20.126743477666256 29.299358645322513 30.56917341441885 20.004724462592378 46-47 20.257251974291144 29.051359810675503 30.32753109080052 20.36385712423283 48-49 20.263625493507405 29.12228326703502 30.40035821692941 20.213733022528167 50-51 20.189560591581067 29.38324394543243 30.11500711443828 20.312188348548226 52-53 20.34861351141835 29.47358595233523 29.8411885610247 20.336611975221714 54-55 20.397774525989025 29.29133978551694 30.027912568329306 20.282973120164726 56-57 20.34349404189747 29.502162933661275 29.894858476859916 20.259484547581344 58-59 20.273470229498216 29.471552477682273 30.077035843897658 20.177941448921853 60-61 20.275207334594647 29.634568331507744 29.97537234874888 20.114851985148725 62-63 20.257299181061846 29.51034612162412 29.92255674193568 20.309797955378357 64-65 20.093242641301572 29.325659903456643 30.118384363920825 20.46271309132096 66-67 20.177075259834247 29.526371931758753 29.843569751204026 20.45298305720297 68-69 20.225722541808878 29.50365254609971 29.894705544071805 20.375919368019613 70-71 20.248105430121214 29.080548370500786 30.018656417504992 20.65268978187301 72-73 20.326157338228807 29.36839146707652 29.888037399137602 20.417413795557074 74-75 20.15641701684095 29.58347852827264 29.71515557389201 20.544948880994397 76-77 20.07177044136241 29.50985931534238 29.860169540539268 20.558200702755947 78-79 20.164045387240286 29.63382840238683 29.6631336391616 20.53899257121128 80-81 20.186563082077242 29.441458036448783 29.678054009664166 20.693924871809806 82-83 20.291676094153868 29.437416107203763 29.81371048491679 20.45719731372558 84-85 20.455510133912348 29.34899012520295 29.634265591939048 20.561234148945655 86-87 20.0454721599347 29.50495657909552 29.687171998924466 20.76239926204531 88-89 20.224295659521836 29.503348877305157 29.607474603925304 20.6648808592477 90-91 20.25077561482616 29.357758244037875 29.909213813936276 20.482252327199696 92-93 20.33163674033885 29.49597439585741 29.54738020439404 20.625008659409698 94-95 20.022083108074025 29.44626348980597 29.62596180691471 20.905691595205298 96-97 20.09031919138963 29.346313609132224 29.771571796528917 20.791795402949226 98-99 19.9649137637998 29.448036460133366 29.650764434533695 20.936285341533146 100-101 20.01874367973562 29.42837637536234 29.537048515121928 21.01583142978011 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 193.0 1 137.5 2 123.0 3 193.0 4 187.5 5 183.0 6 315.5 7 676.0 8 1151.0 9 1497.5 10 1525.5 11 1452.0 12 1580.0 13 1792.0 14 2084.5 15 2458.5 16 2851.0 17 3308.5 18 3784.0 19 4321.5 20 5119.0 21 6307.5 22 7999.5 23 10650.5 24 14119.5 25 18104.0 26 24641.5 27 32744.0 28 39933.0 29 47958.5 30 56161.5 31 63681.5 32 72530.0 33 82475.0 34 91401.0 35 99345.0 36 110877.5 37 120075.0 38 120773.5 39 120053.0 40 121110.5 41 119542.0 42 116534.0 43 115863.0 44 114851.0 45 113447.0 46 110206.0 47 106103.0 48 103013.0 49 97687.0 50 89454.0 51 79953.5 52 70256.0 53 61162.0 54 52179.5 55 42644.0 56 33194.5 57 25128.5 58 19910.5 59 16202.5 60 12128.0 61 8159.0 62 5159.0 63 3007.0 64 1668.0 65 934.5 66 501.0 67 263.5 68 147.0 69 90.5 70 58.0 71 36.5 72 24.5 73 15.5 74 11.5 75 13.0 76 9.5 77 6.0 78 1.5 79 1.0 80 2.5 81 1.5 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004226829426831274 2 0.006855277977970049 3 0.0015628613006771098 4 0.17191474307448204 5 0.0015983808756924985 6 7.814306503385549E-4 7 1.0655872504616657E-4 8 5.683132002462217E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.48637025107722E-4 24-25 4.439946876923607E-4 26-27 0.0023620517385233588 28-29 1.9535766258463872E-4 30-31 0.005736411364985301 32-33 0.01966008477101773 34-35 0.01255616976793996 36-37 9.590285254154991E-4 38-39 3.196761751384997E-4 40-41 0.001545101513169415 42-43 0.011614901030032156 44-45 0.007601189053293215 46-47 0.01022963760443199 48-49 0.010478274629539713 50-51 0.18535890221780674 52-53 0.11398231622438285 54-55 0.017333552607509763 56-57 0.006784238827939272 58-59 0.017422351545048235 60-61 0.12321740572838395 62-63 0.23840738750329 64-65 0.24401948035572144 66-67 0.25447999519775344 68-69 0.2498446906582452 70-71 0.23670244790255135 72-73 0.16299932974561945 74-75 0.2608024795504927 76-77 0.20233725907516262 78-79 0.0054167351898468 80-81 3.551957501538886E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 8.879893753847214E-5 90-91 0.0057896907275083835 92-93 0.017475630907571316 94-95 0.0026639681261541644 96-97 1.775978750769443E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2815349.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.4287558641989 #Duplication Level Percentage of deduplicated Percentage of total 1 71.70901530317467 39.03032487198597 2 16.347247561383014 17.795206931402742 3 5.43814646547875 8.87974638969897 4 2.4630380380023356 5.362403842186582 5 1.2386472661468357 3.3709014825481756 6 0.7290510123313939 2.38088037376395 7 0.4545619620718656 1.731886944113257 8 0.3086561527628465 1.3439816307769483 9 0.20058649821863497 0.9825906187076985 >10 0.9866283514239259 9.556919602977452 >50 0.07638808653725095 2.8909355020455014 >100 0.044431605006439744 4.49326882613108 >500 0.002619416336046728 0.9530553176578768 >1k 8.513103091476636E-4 0.7573045488993275 >5k 1.3097081679194823E-4 0.47059311710442986 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7166 0.2545332745602765 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6037 0.21443167436790253 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3769 0.1338732782330006 No Hit GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT 3030 0.10762431229662822 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.103915003077771E-5 3.551957501538886E-5 12-13 0.0 0.0 0.0 1.0655872504616657E-4 3.551957501538886E-5 14-15 0.0 0.0 0.0 1.0655872504616657E-4 3.551957501538886E-5 16-17 0.0 0.0 0.0 1.0655872504616657E-4 3.551957501538886E-5 18-19 0.0 0.0 0.0 1.0655872504616657E-4 3.551957501538886E-5 20-21 0.0 0.0 0.0 1.4207830006155543E-4 3.551957501538886E-5 22-23 0.0 0.0 0.0 1.5983808756924985E-4 3.551957501538886E-5 24-25 0.0 0.0 0.0 2.8415660012311085E-4 3.551957501538886E-5 26-27 0.0 0.0 0.0 4.262349001846663E-4 3.551957501538886E-5 28-29 0.0 0.0 0.0 7.45911075323166E-4 3.551957501538886E-5 30-31 0.0 0.0 0.0 0.0027172474886772474 3.551957501538886E-5 32-33 0.0 0.0 0.0 0.008045183740985576 3.551957501538886E-5 34-35 0.0 0.0 0.0 0.014651824693847904 3.551957501538886E-5 36-37 0.0 0.0 0.0 0.026000328911264643 3.551957501538886E-5 38-39 0.0 0.0 0.0 0.044612586219328405 3.551957501538886E-5 40-41 0.0 0.0 0.0 0.06468114610302311 3.551957501538886E-5 42-43 0.0 0.0 0.0 0.08522922024942556 3.551957501538886E-5 44-45 0.0 0.0 0.0 0.11050139787287473 3.551957501538886E-5 46-47 0.0 0.0 0.0 0.14191846197398617 3.551957501538886E-5 48-49 0.0 0.0 0.0 0.17653228782648261 3.551957501538886E-5 50-51 0.0 0.0 0.0 0.21203410305436377 3.551957501538886E-5 52-53 0.0 0.0 0.0 0.24803319233246038 3.551957501538886E-5 54-55 0.0 0.0 0.0 0.28687384761178814 3.551957501538886E-5 56-57 0.0 0.0 0.0 0.33484303367007073 3.551957501538886E-5 58-59 0.0 0.0 0.0 0.391088280706939 3.551957501538886E-5 60-61 0.0 0.0 0.0 0.45029941225759224 3.551957501538886E-5 62-63 0.0 0.0 0.0 0.5117127574591995 3.551957501538886E-5 64-65 0.0 0.0 0.0 0.5736056169235146 3.551957501538886E-5 66-67 0.0 0.0 0.0 0.6477172101931234 3.551957501538886E-5 68-69 0.0 0.0 0.0 0.7362497509189803 5.3279362523083285E-5 70-71 0.0 0.0 0.0 0.832685397085761 7.103915003077771E-5 72-73 0.0 0.0 0.0 0.9313765362660189 7.103915003077771E-5 74-75 0.0 0.0 0.0 1.0373491883244315 7.103915003077771E-5 76-77 0.0 0.0 0.0 1.1572810333638919 7.103915003077771E-5 78-79 0.0 0.0 0.0 1.2944576320733239 7.103915003077771E-5 80-81 0.0 0.0 0.0 1.4462327761140803 7.103915003077771E-5 82-83 0.0 0.0 0.0 1.6094097037347768 7.103915003077771E-5 84-85 0.0 0.0 0.0 1.771254647292396 7.103915003077771E-5 86-87 0.0 0.0 0.0 1.9459576770055862 7.103915003077771E-5 88-89 0.0 0.0 0.0 2.147460226067887 7.103915003077771E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3775 0.0 59.302 1 GTATCAA 7200 0.0 41.720547 1 ATCAACG 8715 0.0 33.59113 3 TCAACGC 8935 0.0 32.71027 4 CAACGCA 9025 0.0 32.382 5 TATCAAC 9345 0.0 31.532223 2 AACGCAG 9330 0.0 31.475414 6 ACGCAGA 10835 0.0 26.927803 7 CGCAGAG 10850 0.0 26.803127 8 GGCGTGC 1000 0.0 26.092604 8 ACGCTCG 110 9.56173E-5 25.876963 8 GCGTGCG 990 0.0 24.438486 9 TACATGG 7095 0.0 23.914318 2 ACATGGG 7070 0.0 23.190546 3 GCAGAGT 12650 0.0 22.463406 9 GTACATG 8000 0.0 22.041916 1 TGGTATC 1760 0.0 21.873312 2 AGGCGTG 1475 0.0 21.549515 7 GTGGTAT 1835 0.0 21.498463 1 GAGTACT 9185 0.0 21.305124 12-13 >>END_MODULE