FastQCFastQC Report
Sat 18 Jun 2016
SRR1931047_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931047_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326287
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN141814.346173767266241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2650.056.1254541
CGTAGTG300.00815031850.4163785
GTATCAA4650.047.4267351
CAACGCA6150.035.2504775
TCAACGC6250.034.694434
ATCAACG6200.034.160873
AACGCAG6350.033.9228326
TATCAAC6600.032.6219862
GGCATCG702.6435876E-432.4865467
GCATCGT550.003600834932.2626348
GTACTAG1002.9236966E-530.7722281
ACCGCAT600.005511674629.5740818
TATCGGG300.00564882329.43149888-89
ACGCAGA7450.029.3033037
GTCTAAA905.699605E-428.4928051
CGCAGAG7650.028.4143148
GCAGAGT7950.028.0671569
CCCCTAG750.0080671827.3530921
GTACATG6600.027.1976761
GTAGTGC700.00898085526.7596
Produced by FastQC (version 0.11.5)