FastQCFastQC Report
Sat 18 Jun 2016
SRR1931047_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931047_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326287
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7030.2154544925173237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6200.19001676438227696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.11707484515166096No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA3420.10481569906248181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3800.056.302671
GTATCAA6600.039.6203961
ACGTGCG500.001626211237.9423149
CGTAGTA753.056811E-431.6963183
TACGTGC600.003976301831.6185958
GTGGTAT1701.05501385E-1030.7640721
TCCGATA650.005811359729.258142
ATCAACG9100.028.7356743
TATCAAC9500.027.525752
TACGGGC700.00834470827.1682724
GGCGTCG700.00844526927.101658
CGGGCGT700.00844526927.101656
CAACGCA9800.026.6176955
TCAACGC10150.025.7630184
AACGCAG10600.025.0562466
ACTACGT950.001235565824.9620486
CCGATAC1000.001642138323.772243
ATACTGC1201.7248343E-423.7139476
TACTACG1000.001665781123.7139455
TGGTATC2252.764864E-923.2439672
Produced by FastQC (version 0.11.5)