##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931047_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 326287 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.294997961916962 31.0 31.0 34.0 30.0 34.0 2 31.48551122171585 31.0 31.0 34.0 30.0 34.0 3 31.54642385384646 31.0 31.0 34.0 30.0 34.0 4 35.31163362315998 37.0 35.0 37.0 33.0 37.0 5 35.18723700300656 37.0 35.0 37.0 32.0 37.0 6 35.21283410004076 37.0 35.0 37.0 32.0 37.0 7 35.08906269633788 37.0 35.0 37.0 32.0 37.0 8 35.09602895610306 37.0 35.0 37.0 32.0 37.0 9 36.59129539331938 39.0 35.0 39.0 32.0 39.0 10-11 36.526049153046245 38.0 35.0 39.0 32.0 39.0 12-13 36.40083729967789 38.0 35.0 39.0 32.0 39.0 14-15 37.45406804439037 39.0 36.0 41.0 32.0 41.0 16-17 37.400258055025176 39.0 36.0 41.0 32.0 41.0 18-19 37.421290152534425 39.0 36.0 41.0 32.0 41.0 20-21 37.404469071706814 39.0 36.0 41.0 32.0 41.0 22-23 37.298707886002205 39.0 36.0 40.5 31.0 41.0 24-25 37.15922638658604 39.0 36.0 40.0 31.0 41.0 26-27 36.893927738463375 39.0 36.0 40.0 30.5 41.0 28-29 36.71989536818813 39.0 35.5 40.0 30.0 41.0 30-31 36.491230113366456 38.0 35.0 40.0 30.0 41.0 32-33 36.347526870515836 38.0 35.0 40.0 30.0 41.0 34-35 36.459802259973586 38.0 35.0 40.0 30.0 41.0 36-37 36.52609359245082 38.0 35.0 40.0 30.0 41.0 38-39 36.52748500553194 38.5 35.0 40.0 30.0 41.0 40-41 36.41943289190191 38.5 35.0 40.0 30.0 41.0 42-43 36.292299417384086 38.0 35.0 40.0 30.0 41.0 44-45 36.14321594179357 38.0 35.0 40.0 30.0 41.0 46-47 35.917518932718735 38.0 35.0 40.0 29.0 41.0 48-49 35.697563801193425 38.0 34.5 40.0 28.0 41.0 50-51 35.446671182118806 38.0 34.0 40.0 27.5 41.0 52-53 35.2303524198022 38.0 34.0 40.0 27.0 41.0 54-55 35.07825779145354 38.0 34.0 40.0 26.5 41.0 56-57 34.889417905095826 38.0 33.5 40.0 26.0 41.0 58-59 34.67241416299148 38.0 33.0 40.0 26.0 41.0 60-61 34.36161569415883 37.0 33.0 40.0 26.0 41.0 62-63 34.10405563200495 37.0 33.0 40.0 25.5 41.0 64-65 33.775942651714594 36.5 32.0 39.0 25.0 41.0 66-67 33.44947086460692 36.0 32.0 39.0 24.0 40.5 68-69 33.104553659814826 36.0 31.5 39.0 23.0 40.0 70-71 32.739001247368115 35.0 31.0 38.5 22.5 40.0 72-73 32.30771989077101 35.0 31.0 38.0 22.0 40.0 74-75 31.793316926509483 35.0 31.0 37.0 21.0 39.0 76-77 30.41281448540702 33.5 28.5 35.5 20.0 38.5 78-79 31.16467251223616 34.0 30.0 36.0 20.5 39.0 80-81 31.148594642140203 34.0 30.0 36.0 21.0 37.5 82-83 30.881037246350605 34.0 30.0 35.5 20.0 37.0 84-85 30.55141792348454 34.0 30.0 35.0 20.0 37.0 86-87 30.206614115793766 34.0 30.0 35.0 18.0 36.0 88-89 29.859399853503206 34.0 29.5 35.0 17.5 36.0 90-91 29.529483246344476 34.0 29.0 35.0 12.5 36.0 92-93 29.213652398042214 34.0 29.0 35.0 7.0 35.0 94-95 28.835535893247354 34.0 29.0 35.0 2.0 35.0 96-97 28.421063358331775 34.0 29.0 35.0 2.0 35.0 98-99 27.91437599414013 33.0 29.0 35.0 2.0 35.0 100-101 26.74730222166375 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3929875495112256 1101 2 0.46204053335045714 1101 3 0.36436375553492084 1101 4 0.38679067266288314 1101 5 0.3661232320365997 1101 6 0.5229179262430037 1101 7 0.5435684317674543 1101 8 0.5245842266037997 1101 9 0.9426786603380535 1101 10-11 0.6924095264030328 1101 12-13 0.542076677197123 1101 14-15 0.41133891577857895 1101 16-17 0.7311570276433699 1101 18-19 0.5533601092323295 1101 20-21 0.6005424069628376 1101 22-23 0.5910319836345437 1101 24-25 0.4645325186113354 1101 26-27 0.364700829673275 1101 28-29 0.43422799723492744 1101 30-31 0.5817215785807619 1101 32-33 0.6340553032076173 1101 34-35 1.0397905523217048 1101 36-37 0.7942165598145507 1101 38-39 0.9562855244043149 1101 40-41 0.9486653932287652 1101 42-43 0.7303255757777123 1101 44-45 0.8985827939500624 1101 46-47 0.9449817751233525 1101 48-49 0.6250492907533456 1101 50-51 0.856203465562551 1101 52-53 0.8513018028492283 1101 54-55 0.8853857699545813 1101 56-57 0.6480596046488287 1101 58-59 0.6923866344696492 1101 60-61 0.5243741772704027 1101 62-63 0.32021791274573985 1101 64-65 0.7131830412366824 1101 66-67 0.6849330693681352 1101 68-69 0.7479907094478548 1101 70-71 0.3881393976563601 1101 72-73 0.589317714152223 1101 74-75 0.43545147951329355 1101 76-77 0.8074298884844353 1101 78-79 0.777663571245629 1101 80-81 0.5345155601510001 1101 82-83 0.8882873104181215 1101 84-85 0.9686978139887295 1101 86-87 1.0325100973361607 1101 88-89 1.001427177326871 1101 90-91 0.665715739900719 1101 92-93 0.1897414268961377 1101 94-95 0.47203207891488574 1101 96-97 0.6423581940515746 1101 98-99 0.39408577833394176 1101 100-101 0.6961468429226123 1102 1 0.4028808247106248 1102 2 0.34116747370188705 1102 3 0.19798162996912083 1102 4 -0.3128214759288568 1102 5 -0.14225978655050397 1102 6 0.009194290070389854 1102 7 0.06445374857113961 1102 8 0.04882275084232646 1102 9 0.5591085012576897 1102 10-11 0.2859316199251225 1102 12-13 0.11891590709758049 1102 14-15 0.40573126731379006 1102 16-17 0.44816558138661833 1102 18-19 0.45461559212046154 1102 20-21 0.26026627076833364 1102 22-23 0.23650252706426755 1102 24-25 0.46221241425041626 1102 26-27 0.39161841781535855 1102 28-29 0.43259079151609114 1102 30-31 0.8469532825879753 1102 32-33 0.6893044298853113 1102 34-35 0.635054912892187 1102 36-37 0.43645208980517936 1102 38-39 0.6093336162279215 1102 40-41 0.7691068259150953 1102 42-43 0.9600334177304504 1102 44-45 0.7534361896197836 1102 46-47 0.7646352478380578 1102 48-49 0.5139907103070058 1102 50-51 0.2755382522442744 1102 52-53 0.17048249958094175 1102 54-55 0.49127445747591736 1102 56-57 0.5957697054201461 1102 58-59 0.6680167835691861 1102 60-61 0.35803582725858263 1102 62-63 -0.022448368696046828 1102 64-65 0.09541366836527487 1102 66-67 -0.04005576786560283 1102 68-69 0.10082167452372204 1102 70-71 0.16766819759332918 1102 72-73 0.0025852090523770244 1102 74-75 0.5704596655418861 1102 76-77 0.4918694300668349 1102 78-79 0.7122278731568734 1102 80-81 0.613810598629712 1102 82-83 0.1370112530196188 1102 84-85 0.5989169544119513 1102 86-87 0.4375477880881391 1102 88-89 0.4889248791102858 1102 90-91 0.5115119734520555 1102 92-93 0.9808094628621369 1102 94-95 1.380129680890036 1102 96-97 1.100841458657289 1102 98-99 1.2965713542889041 1102 100-101 1.3156978808409967 1103 1 0.3689547958273103 1103 2 0.3620248199626488 1103 3 0.4254801044419665 1103 4 0.28021025503595354 1103 5 0.2559112758944124 1103 6 0.3690576706385613 1103 7 0.27039991441289146 1103 8 0.19957025914796844 1103 9 0.28273383178856903 1103 10-11 0.24731035746665242 1103 12-13 0.42126691394550875 1103 14-15 0.6445779687850717 1103 16-17 0.5446085605716533 1103 18-19 0.315551079272133 1103 20-21 0.21337326107276056 1103 22-23 0.43415999536720307 1103 24-25 0.34716224135733853 1103 26-27 0.3371020099232922 1103 28-29 0.24609509689272357 1103 30-31 0.37073879347006766 1103 32-33 0.14857709243870687 1103 34-35 0.3355557943078793 1103 36-37 0.35952314546997144 1103 38-39 0.4753118181399145 1103 40-41 0.6741088599513034 1103 42-43 0.5784906012683209 1103 44-45 0.6563914747238684 1103 46-47 1.0125842613304883 1103 48-49 0.42765858609519825 1103 50-51 0.1949099045596867 1103 52-53 0.7273973751657294 1103 54-55 0.47201891472726487 1103 56-57 0.27021373552388894 1103 58-59 0.7280123764907387 1103 60-61 1.1233292369812773 1103 62-63 0.560078412780662 1103 64-65 0.7411354122123086 1103 66-67 0.5125833941114806 1103 68-69 0.5942788580615925 1103 70-71 0.5166749099128012 1103 72-73 0.49433464969241925 1103 74-75 0.28163923703825944 1103 76-77 0.2697526763886202 1103 78-79 0.559963312847696 1103 80-81 0.5403818915987273 1103 82-83 0.3884138904865644 1103 84-85 0.5506213072494326 1103 86-87 0.4241400994312805 1103 88-89 0.36591230198641256 1103 90-91 0.5419335274557149 1103 92-93 0.3219215721576809 1103 94-95 0.38547750108123324 1103 96-97 0.3901364956089495 1103 98-99 0.1507945219612914 1103 100-101 0.41157302491856385 1104 1 0.22810153673788847 1104 2 0.3329959958552138 1104 3 0.3391469894730825 1104 4 0.10981228582534186 1104 5 0.3384448494286403 1104 6 0.2331831210152444 1104 7 0.04514606573945201 1104 8 0.201740255872501 1104 9 0.5819968517689205 1104 10-11 0.11571029195732052 1104 12-13 0.20794395292355716 1104 14-15 0.345052911464407 1104 16-17 0.6118743646987355 1104 18-19 0.36008514412637993 1104 20-21 0.495268118589955 1104 22-23 0.4813758486262927 1104 24-25 0.5201642066373893 1104 26-27 0.4379741029465478 1104 28-29 0.5907478974167972 1104 30-31 0.3728309978591895 1104 32-33 0.19318485529491625 1104 34-35 0.23552713397377545 1104 36-37 0.4376839708875977 1104 38-39 0.5565392993059746 1104 40-41 0.5193839991586415 1104 42-43 0.5064876533482376 1104 44-45 0.8968148288018156 1104 46-47 0.24078603008253907 1104 48-49 0.0875709673595253 1104 50-51 0.12530214170347875 1104 52-53 0.13793209511332094 1104 54-55 0.5842691603872723 1104 56-57 0.26648789209119883 1104 58-59 0.30509229787953984 1104 60-61 0.11756032116077364 1104 62-63 0.16113065647538605 1104 64-65 0.07017242498663023 1104 66-67 0.3354215860538332 1104 68-69 0.3303745016908053 1104 70-71 0.1696392073251829 1104 72-73 0.28551954979068483 1104 74-75 0.3514435278997077 1104 76-77 0.21891743236634653 1104 78-79 0.555754338068791 1104 80-81 0.7148987602525096 1104 82-83 0.6603636120718903 1104 84-85 0.8077495417728535 1104 86-87 0.9337535943543109 1104 88-89 0.6585138414557896 1104 90-91 0.7934279591622335 1104 92-93 0.7656285816565322 1104 94-95 1.1197798266626293 1104 96-97 0.7943905080557201 1104 98-99 1.0183551180962418 1104 100-101 0.8798460350725072 1105 1 0.1914818184280307 1105 2 0.30764388317915703 1105 3 0.35604839792378584 1105 4 0.3746010182197068 1105 5 0.006050483231454962 1105 6 0.4360000224236913 1105 7 0.41697705165494625 1105 8 0.4073740586894061 1105 9 0.4073489644449779 1105 10-11 0.6058511370277344 1105 12-13 0.378366488134823 1105 14-15 0.37181347484468574 1105 16-17 0.5766630970931033 1105 18-19 0.6037471159573684 1105 20-21 0.6501976960547395 1105 22-23 0.6686997922882654 1105 24-25 0.645516319313451 1105 26-27 0.3576924128057115 1105 28-29 0.4724380382618705 1105 30-31 0.7587464908169395 1105 32-33 0.4776918975484392 1105 34-35 0.7425693874949033 1105 36-37 0.6278248159580144 1105 38-39 0.4297787359257015 1105 40-41 0.4292431540882191 1105 42-43 0.5149383575735911 1105 44-45 0.7263922935905569 1105 46-47 0.38726490332197727 1105 48-49 0.7171484321482566 1105 50-51 0.9337528459288365 1105 52-53 0.5646926584935983 1105 54-55 0.6363818364436042 1105 56-57 0.7383188780066874 1105 58-59 0.6825570866119293 1105 60-61 1.103475814118518 1105 62-63 1.0780320649260986 1105 64-65 0.8293273545641 1105 66-67 1.074858205772145 1105 68-69 0.7669942200006687 1105 70-71 0.8048504749308165 1105 72-73 0.7545336342977222 1105 74-75 0.9824294433926646 1105 76-77 0.9682132070142337 1105 78-79 0.9923740563786545 1105 80-81 0.661377633491945 1105 82-83 0.7279692458747071 1105 84-85 0.6992988375474987 1105 86-87 0.5253028901289589 1105 88-89 0.6740067992022674 1105 90-91 0.4807519028242062 1105 92-93 1.024783511234002 1105 94-95 0.9380896858175589 1105 96-97 0.8577707897458602 1105 98-99 0.6366649772511686 1105 100-101 0.6897051900020834 1106 1 0.27057051491288675 1106 2 0.22997560723453958 1106 3 0.19116789678665214 1106 4 0.18301182299168062 1106 5 0.5226366860004745 1106 6 0.43392525084806266 1106 7 0.3603597273122858 1106 8 0.18075314339594684 1106 9 0.21426752966063134 1106 10-11 0.10078390644880386 1106 12-13 0.3401268520178391 1106 14-15 0.49959147428609185 1106 16-17 0.2676099111217525 1106 18-19 0.6574996595475184 1106 20-21 0.23247036891913098 1106 22-23 0.2377537762486881 1106 24-25 0.18381979455583775 1106 26-27 0.6068126298587302 1106 28-29 0.33472906487480714 1106 30-31 0.30678942262877484 1106 32-33 0.7258425578843912 1106 34-35 0.5181229924105111 1106 36-37 0.389325833394075 1106 38-39 0.5582430059652026 1106 40-41 0.5983051179393115 1106 42-43 0.5988229683544191 1106 44-45 0.6335103560730957 1106 46-47 0.7078211017020593 1106 48-49 0.6868447973080549 1106 50-51 0.7277719178675426 1106 52-53 0.5027407772343722 1106 54-55 0.6449881485371307 1106 56-57 0.710748355722842 1106 58-59 0.6719398420681841 1106 60-61 0.5184021996270332 1106 62-63 0.24353499753591734 1106 64-65 0.16703632795115908 1106 66-67 -0.36986110157334195 1106 68-69 0.17162255659246028 1106 70-71 -0.016232396233846202 1106 72-73 0.25929363908566216 1106 74-75 0.5675070478294906 1106 76-77 0.004386051446264361 1106 78-79 0.2907262089541618 1106 80-81 0.5795478445600537 1106 82-83 0.7441644340006306 1106 84-85 0.588613763933008 1106 86-87 0.4655295190241411 1106 88-89 0.358150756430053 1106 90-91 0.002022301419746242 1106 92-93 0.1788991398498858 1106 94-95 0.5979667955011578 1106 96-97 0.28122368844115186 1106 98-99 0.6479246029942978 1106 100-101 1.1820165653362444 1107 1 0.31566022218389733 1107 2 0.2577612540711769 1107 3 0.19912351529467998 1107 4 0.2262050870772967 1107 5 0.2646968056101713 1107 6 0.23830831663339325 1107 7 0.5377125759115984 1107 8 0.32818783020740483 1107 9 0.3167777594371515 1107 10-11 0.30021733421083496 1107 12-13 0.20821781833826947 1107 14-15 0.280107684547346 1107 16-17 0.32177264325898847 1107 18-19 0.24558983896205433 1107 20-21 0.42768204660247733 1107 22-23 0.6987271788297349 1107 24-25 0.42384182792064706 1107 26-27 0.3213074362798878 1107 28-29 0.5808496188565471 1107 30-31 0.3833356926948781 1107 32-33 0.3068781260304405 1107 34-35 0.23791367544482966 1107 36-37 0.6326174607154513 1107 38-39 0.5336128517316467 1107 40-41 0.24536208992545738 1107 42-43 -0.16986211190997125 1107 44-45 -0.13272351235624313 1107 46-47 -0.14368188854030706 1107 48-49 -0.13564890744485325 1107 50-51 0.19077553763462163 1107 52-53 0.2603108118582327 1107 54-55 0.11597886304767258 1107 56-57 0.09340494999416649 1107 58-59 0.027334081917096853 1107 60-61 0.16063543853167062 1107 62-63 0.1723200148478483 1107 64-65 0.15008244063608345 1107 66-67 -0.17107293663787715 1107 68-69 -0.14018496622781385 1107 70-71 0.0773661243830901 1107 72-73 0.038003900338409835 1107 74-75 0.3565100380718498 1107 76-77 0.2670394980940465 1107 78-79 0.39294134902340616 1107 80-81 0.10989093552637641 1107 82-83 0.19174076387157868 1107 84-85 -0.05784122505030709 1107 86-87 -0.0121971098710425 1107 88-89 -0.10656831816862677 1107 90-91 -0.040140115955136224 1107 92-93 -0.07245827280355854 1107 94-95 0.1225576044404093 1107 96-97 -0.01777694108356087 1107 98-99 0.4007299224780887 1107 100-101 0.37035855463432554 1108 1 0.32950998744211546 1108 2 0.2597565592354947 1108 3 0.16168220074068884 1108 4 0.3154460886422399 1108 5 0.16943076492160003 1108 6 0.4247324167898867 1108 7 0.5268362065845196 1108 8 0.322867016435886 1108 9 0.36509544331821786 1108 10-11 0.41148493984464096 1108 12-13 0.4135777557404552 1108 14-15 0.3478698128728581 1108 16-17 0.6146912661071866 1108 18-19 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-0.9127675051604953 2316 90-91 -1.0336360509144846 2316 92-93 -1.1075192484133147 2316 94-95 -1.8513583847194823 2316 96-97 -1.692641439728547 2316 98-99 -2.0213906737278755 2316 100-101 -1.6727647515987378 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 2.0 5 3.0 6 19.0 7 37.0 8 108.0 9 203.0 10 327.0 11 535.0 12 779.0 13 978.0 14 1228.0 15 1551.0 16 1839.0 17 2184.0 18 1959.0 19 1646.0 20 1510.0 21 1696.0 22 1778.0 23 2245.0 24 2759.0 25 3461.0 26 4559.0 27 5809.0 28 7565.0 29 9271.0 30 11568.0 31 13971.0 32 16736.0 33 20189.0 34 24937.0 35 31354.0 36 40493.0 37 55222.0 38 49604.0 39 8159.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.02004474821467 23.169767370582647 15.769761240690224 23.04042664051246 2 14.680217857044342 21.999638335238714 40.810496859952984 22.50964694776396 3 17.881212819624803 27.022106711699422 31.318096977758437 23.778583490917338 4 12.254459488502041 18.27975806699211 39.57109084768659 29.894691596819257 5 11.897267726067701 39.032747444718574 36.4377767902294 12.632208038984324 6 29.02532762869157 40.19228647435976 17.690110455921833 13.092275441026835 7 25.480635146358882 33.453370805456544 23.34080119649266 17.725192851691915 8 24.131051074980462 37.87149271342538 20.48117443339412 17.516281778200042 9 24.59797662793798 16.357378626791753 21.561386141648303 37.48325860362197 10-11 23.851547870433084 27.705670161544898 29.058466932485814 19.3843150355362 12-13 24.187754951928824 24.85848348233304 29.636025952612272 21.31773561312587 14-15 21.412437516664777 26.69766187436214 26.76784548572269 25.12205512325039 16-17 20.723320267126795 29.75371375506839 27.875152856227796 21.647813121577016 18-19 20.706310701928057 28.681651429569673 29.946795305972962 20.665242562529308 20-21 21.3960409087705 28.05076512395529 29.73271996739067 20.82047399988354 22-23 21.321325462937423 27.606302446320097 29.768056245134638 21.304315845607842 24-25 21.09618387521396 27.904157849006932 29.92952512592461 21.0701331498545 26-27 20.899865760293242 28.14451915827413 29.801368141668867 21.154246939763762 28-29 20.89130258223984 28.126508451927982 29.869761697158786 21.11242726867339 30-31 21.318713315862873 27.873201232127258 29.779626990329945 21.02845846167992 32-33 21.32852877523619 28.022212140347865 29.469710439031566 21.179548645384376 34-35 21.29450403603388 28.005799260379742 29.719830129487185 20.97986657409919 36-37 21.2169134604923 27.91076499190124 29.78535603131623 21.08696551629023 38-39 21.15398760596532 28.123180277425323 29.723769944159418 20.999062172449936 40-41 21.307084176797172 27.892308375998176 29.815176066212356 20.9854313809923 42-43 21.20875887350882 28.036149969961873 29.47031742334147 21.28477373318784 44-45 21.068400234779556 28.212778934818022 29.503453496943422 21.215367333459 46-47 21.185478296984122 28.027947335915147 29.180874557288515 21.60569980981222 48-49 21.346821677389297 28.045222018224898 29.157688570666686 21.450267733719123 50-51 21.405168045938186 28.263499715957067 28.70476424436904 21.6265679937357 52-53 21.315816524571453 28.427123335169295 28.690397104602226 21.566663035657026 54-55 21.365108412610105 28.194022324705998 28.800035557680843 21.640833705003057 56-57 21.384414039991665 28.44364142361494 28.621562641754632 21.55038189463876 58-59 21.128474563802 28.371261816888804 28.99720440918621 21.50305921012298 60-61 21.374990985904255 28.526736191556058 28.81948333504665 21.27878948749304 62-63 21.4093035973787 28.233232049206265 28.713435559638327 21.64402879377671 64-65 21.022165141673785 28.30879415975862 28.807156507899446 21.86188419066815 66-67 21.136853555893918 28.39752384961796 28.68253619503141 21.78308639945671 68-69 21.22567878169594 28.33506434444868 28.705029560343664 21.73422731351172 70-71 21.160353983388404 28.133261442938533 28.820367716297152 21.88601685737591 72-73 21.37011364403814 28.391823277688072 28.64139811023042 21.59666496804337 74-75 21.206612814748684 28.5990869656744 28.552221353202306 21.642078866374614 76-77 21.078309423114135 28.486945358161876 28.758757098586273 21.675988120137717 78-79 20.974620751799502 28.490470330599916 28.697047388593205 21.837861529007373 80-81 21.01585414067983 28.419918660565695 28.63338104184353 21.930846156910942 82-83 21.208016255627715 28.417007113369518 28.917793231112487 21.45718339989028 84-85 21.32984151988893 28.338395339072658 28.489336075295675 21.842427065742736 86-87 20.84897651454088 28.470181159531332 28.69743508015949 21.983407245768298 88-89 21.164802765663357 28.30943310643697 28.67184411269833 21.853920015201343 90-91 21.202355119104542 28.32012333419152 28.892505547586644 21.585015999117292 92-93 21.14051785708812 28.398762832243605 28.739167885135902 21.721551425532372 94-95 20.73409566659209 28.31487775799239 28.849083146632175 22.10194342878334 96-97 20.95409869225559 28.193890654546454 28.826309353421987 22.025701299775964 98-99 20.75657320089369 28.31586915813379 28.75827109262704 22.169286548345475 100-101 20.802391759402 28.199713748938816 28.721340415033392 22.276554076625793 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 36.0 1 21.0 2 14.5 3 23.5 4 22.0 5 22.0 6 37.0 7 102.0 8 179.0 9 210.0 10 170.0 11 134.0 12 141.5 13 159.5 14 180.5 15 203.5 16 251.0 17 305.0 18 347.5 19 394.0 20 435.0 21 473.5 22 518.5 23 622.5 24 821.0 25 1024.5 26 1310.5 27 1686.5 28 2129.5 29 2810.5 30 3639.0 31 4583.0 32 5719.5 33 7060.5 34 8500.5 35 9685.0 36 11241.5 37 12942.5 38 13629.0 39 13832.0 40 13750.5 41 13344.5 42 13347.5 43 13795.0 44 14187.0 45 14491.5 46 14483.5 47 14245.0 48 14077.0 49 13731.5 50 13098.0 51 12124.0 52 10751.0 53 9402.5 54 8322.5 55 7000.0 56 5478.5 57 4140.0 58 3193.5 59 2520.5 60 1850.5 61 1243.0 62 842.0 63 548.5 64 295.0 65 169.5 66 111.0 67 62.5 68 32.5 69 16.0 70 6.0 71 2.5 72 2.5 73 5.5 74 4.5 75 3.5 76 4.0 77 1.5 78 0.0 79 0.0 80 0.5 81 1.0 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005210137087901142 2 0.005516615740130621 3 0.0018388719133768737 4 0.17683818233640936 5 6.129573044589578E-4 6 9.194359566884368E-4 7 0.0 8 6.129573044589578E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.064786522294789E-4 24-25 1.5323932611473946E-4 26-27 0.0015323932611473947 28-29 1.5323932611473946E-4 30-31 0.0067425303490485375 32-33 0.02114702700383405 34-35 0.011799428110834941 36-37 7.661966305736973E-4 38-39 3.064786522294789E-4 40-41 0.0010726752828031762 42-43 0.010726752828031764 44-45 0.007202248327392756 46-47 0.009347598892999109 48-49 0.009194359566884368 50-51 0.1936945082090307 52-53 0.11967991369561154 54-55 0.01731604385096556 56-57 0.0058230943923601 58-59 0.01808224048153926 60-61 0.12519652943574217 62-63 0.2451829217835832 64-65 0.25161897348040224 66-67 0.26433783754792556 68-69 0.2608133330472866 70-71 0.24257785323963257 72-73 0.16335312163831228 74-75 0.27230628250589206 76-77 0.2126961846472584 78-79 0.005056897761786403 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0058230943923601 92-93 0.01777576182930978 94-95 0.0024518292178358314 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 326287.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.62923476160773 #Duplication Level Percentage of deduplicated Percentage of total 1 81.9483860626832 55.421066393680654 2 11.335375785259242 15.332055801846817 3 2.8920185646490255 5.867550073307303 4 1.2350204748851707 3.3409395852560593 5 0.6574538500096662 2.2231550383613277 6 0.38841587730957117 1.576096113102289 7 0.29220108932302574 1.3832935247197093 8 0.2035965605357771 1.1015263671303963 9 0.14747640860489694 0.8976344993405426 >10 0.859605613654377 10.136160536646676 >50 0.030905398762392306 1.353959971010625 >100 0.00863533200712872 0.9599130509562148 >500 9.089823165398652E-4 0.4066490446413936 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 703 0.2154544925173237 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 620 0.19001676438227696 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 382 0.11707484515166096 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 342 0.10481569906248181 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 6.12957304458958E-4 0.0 32-33 0.0 0.0 0.0 0.004903658435671664 0.0 34-35 0.0 0.0 0.0 0.011492949458605461 0.0 36-37 0.0 0.0 0.0 0.022985898917210923 0.0 38-39 0.0 0.0 0.0 0.03907602815925856 0.0 40-41 0.0 0.0 0.0 0.057771225945256785 0.0 42-43 0.0 0.0 0.0 0.07723262036182871 0.0 44-45 0.0 0.0 0.0 0.10175091254018702 0.0 46-47 0.0 0.0 0.0 0.12948723056695485 0.0 48-49 0.0 0.0 0.0 0.1592156598332143 0.0 50-51 0.0 0.0 0.0 0.1952269014701781 0.0 52-53 0.0 0.0 0.0 0.23185110041160084 0.0 54-55 0.0 0.0 0.0 0.2726127611581215 0.0 56-57 0.0 0.0 0.0 0.32885159384223095 0.0 58-59 0.0 0.0 0.0 0.3884616917008646 0.0 60-61 0.0 0.0 0.0 0.46079065362702165 0.0 62-63 0.0 0.0 0.0 0.5361844020754735 0.0 64-65 0.0 0.0 0.0 0.6114249111978105 0.0 66-67 0.0 0.0 0.0 0.6969324551698352 0.0 68-69 0.0 0.0 0.0 0.7924005553393179 0.0 70-71 0.0 0.0 0.0 0.9067170926209135 0.0 72-73 0.0 0.0 0.0 1.0283891175560167 3.06478652229479E-4 74-75 0.0 0.0 0.0 1.1568036728401683 3.06478652229479E-4 76-77 0.0 0.0 0.0 1.3095832809765637 3.06478652229479E-4 78-79 0.0 0.0 0.0 1.4919380790531036 3.06478652229479E-4 80-81 0.0 0.0 0.0 1.6962061007640512 4.5971797834421847E-4 82-83 0.0 0.0 0.0 1.9036921483234086 6.12957304458958E-4 84-85 0.0 0.0 0.0 2.117920726231814 6.12957304458958E-4 86-87 0.0 0.0 0.0 2.3560546390141193 6.12957304458958E-4 88-89 0.0 0.0 0.0 2.6200859979098157 6.12957304458958E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 380 0.0 56.30267 1 GTATCAA 660 0.0 39.620396 1 ACGTGCG 50 0.0016262112 37.942314 9 CGTAGTA 75 3.056811E-4 31.696318 3 TACGTGC 60 0.0039763018 31.618595 8 GTGGTAT 170 1.05501385E-10 30.764072 1 TCCGATA 65 0.0058113597 29.25814 2 ATCAACG 910 0.0 28.735674 3 TATCAAC 950 0.0 27.52575 2 TACGGGC 70 0.008344708 27.168272 4 GGCGTCG 70 0.008445269 27.10165 8 CGGGCGT 70 0.008445269 27.10165 6 CAACGCA 980 0.0 26.617695 5 TCAACGC 1015 0.0 25.763018 4 AACGCAG 1060 0.0 25.056246 6 ACTACGT 95 0.0012355658 24.962048 6 CCGATAC 100 0.0016421383 23.77224 3 ATACTGC 120 1.7248343E-4 23.713947 6 TACTACG 100 0.0016657811 23.713945 5 TGGTATC 225 2.764864E-9 23.243967 2 >>END_MODULE