FastQCFastQC Report
Sat 18 Jun 2016
SRR1931046_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931046_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2509828
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1096004.366833105694893No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25780.10271620206643642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23200.057.965271
GTATCAA44850.046.6295281
ATCAACG52350.039.285623
TCAACGC53200.038.7490164
CAACGCA54200.037.9390835
TATCAAC55350.037.315182
AACGCAG58450.034.760186
ACGCAGA68050.029.0944047
CGCAGAG69250.027.8166858
GTGGTAT12600.025.5664331
GTACATG46300.025.0694621
GCAGAGT75250.024.3773549
TGGTATC12750.023.2562482
TACATGG49100.023.2190932
ACATGGG48100.022.789693
GAGTACT49200.022.00402812-13
CAGAGTA72150.021.01190210-11
AGAGTAC66400.020.34452210-11
GAGTACA33750.019.3926121
CATGGGT13100.019.190544
Produced by FastQC (version 0.11.5)