##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1931046_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2509828
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.752150346557613	30.0	2.0	31.0	2.0	33.0
2	20.434009422159605	28.0	2.0	31.0	2.0	33.0
3	21.119152786565454	28.0	2.0	31.0	2.0	34.0
4	22.696028174042205	32.0	2.0	35.0	2.0	37.0
5	22.074672846107383	32.0	2.0	35.0	2.0	37.0
6	23.053227153414497	32.0	2.0	35.0	2.0	37.0
7	23.24942984140746	32.0	2.0	35.0	2.0	37.0
8	23.69477390482535	32.0	2.0	35.0	2.0	37.0
9	25.041313189589086	33.0	8.0	37.0	2.0	39.0
10-11	25.423068433374716	33.0	5.0	37.0	2.0	39.0
12-13	25.34193757500514	33.0	2.0	37.0	2.0	39.0
14-15	25.363963586349342	33.0	2.0	38.0	2.0	40.0
16-17	24.252928288312983	32.0	2.0	38.0	2.0	40.0
18-19	23.217608138884415	31.5	2.0	38.0	2.0	40.0
20-21	22.89310044353637	31.0	2.0	38.0	2.0	40.0
22-23	22.546151170518456	30.0	2.0	38.0	2.0	40.0
24-25	22.39595024041488	30.0	2.0	38.0	2.0	40.0
26-27	22.102896493305515	29.5	2.0	38.0	2.0	40.0
28-29	21.916596475933808	29.0	2.0	37.0	2.0	40.0
30-31	21.918153953179264	29.0	2.0	37.0	2.0	39.5
32-33	21.800756665396992	29.5	2.0	37.0	2.0	40.0
34-35	21.471563190784387	27.0	2.0	37.0	2.0	39.0
36-37	21.018235313336213	26.5	2.0	37.0	2.0	39.0
38-39	20.906123248286338	26.0	2.0	37.0	2.0	39.0
40-41	20.777255453361743	25.5	2.0	36.0	2.0	39.0
42-43	20.635367841939768	25.5	2.0	36.0	2.0	39.0
44-45	20.247764189418557	25.0	2.0	36.0	2.0	39.0
46-47	19.90032563984464	24.0	2.0	36.0	2.0	39.0
48-49	19.644404317746076	23.5	2.0	35.5	2.0	39.0
50-51	17.218727737518268	12.0	2.0	33.5	2.0	37.5
52-53	19.217541002809753	23.0	2.0	34.5	2.0	38.5
54-55	20.05730253228508	25.5	2.0	36.5	2.0	39.0
56-57	20.043090801441373	25.5	2.0	36.5	2.0	39.0
58-59	19.775751764662758	24.5	2.0	36.0	2.0	39.0
60-61	19.48882831811582	23.5	2.0	36.0	2.0	39.0
62-63	19.055740074618658	22.5	2.0	35.5	2.0	39.0
64-65	18.70271648097001	21.5	2.0	35.0	2.0	39.0
66-67	18.33809288923384	20.0	2.0	34.0	2.0	38.0
68-69	17.89396046262931	18.0	2.0	34.0	2.0	38.0
70-71	17.54770107752404	15.5	2.0	34.0	2.0	38.0
72-73	17.146968039244122	10.5	2.0	33.5	2.0	37.0
74-75	16.689862213665634	8.0	2.0	33.0	2.0	36.0
76-77	16.263898163539494	7.0	2.0	32.0	2.0	36.0
78-79	15.885028376446513	3.5	2.0	32.0	2.0	35.5
80-81	15.438489808863396	2.0	2.0	31.5	2.0	35.0
82-83	14.971349630333233	2.0	2.0	31.0	2.0	35.0
84-85	14.563062488744247	2.0	2.0	31.0	2.0	34.0
86-87	14.067978363457577	2.0	2.0	30.5	2.0	34.0
88-89	13.638626830205098	2.0	2.0	30.0	2.0	34.0
90-91	13.140543096977165	2.0	2.0	29.5	2.0	34.0
92-93	12.575479674304376	2.0	2.0	29.0	2.0	34.0
94-95	11.987532811013345	2.0	2.0	29.0	2.0	34.0
96-97	11.323299843654624	2.0	2.0	27.0	2.0	34.0
98-99	10.613997652428772	2.0	2.0	25.5	2.0	34.0
100-101	9.595547583340373	2.0	2.0	21.5	2.0	32.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	8.0249354838175
1101	2	9.688085501077392
1101	3	8.969891864624369
1101	4	11.247532567926939
1101	5	11.88309356163882
1101	6	11.033627360374986
1101	7	10.645392006047167
1101	8	10.04569356164718
1101	9	9.921721182604852
1101	10-11	9.344215136927442
1101	12-13	9.275538180388462
1101	14-15	10.09140809982847
1101	16-17	11.015512908575406
1101	18-19	12.05241044138867
1101	20-21	11.954129162300472
1101	22-23	12.379313258601005
1101	24-25	12.085637473109525
1101	26-27	12.29532817114335
1101	28-29	12.115993988519033
1101	30-31	11.91722525644872
1101	32-33	11.68538025809529
1101	34-35	11.664949617314647
1101	36-37	11.991622439882981
1101	38-39	11.661265317440463
1101	40-41	11.466757645337314
1101	42-43	10.883149479013628
1101	44-45	11.046054855063066
1101	46-47	10.84138422061903
1101	48-49	10.902865804576734
1101	50-51	12.753920600035787
1101	52-53	11.849130097448398
1101	54-55	11.587947860788777
1101	56-57	11.546860322712934
1101	58-59	11.185940204191478
1101	60-61	10.992852871807827
1101	62-63	11.342391921510789
1101	64-65	11.118824708980934
1101	66-67	10.648138736997993
1101	68-69	10.748932533712509
1101	70-71	10.347928243569633
1101	72-73	10.347121187136416
1101	74-75	10.181791843724707
1101	76-77	9.943693434690317
1101	78-79	9.620377765281438
1101	80-81	9.34414805514594
1101	82-83	8.876366340621505
1101	84-85	8.632839837139892
1101	86-87	8.607012247347612
1101	88-89	8.606946965432824
1101	90-91	8.246334262207151
1101	92-93	7.964204996819467
1101	94-95	7.7512194682731295
1101	96-97	7.617625808712699
1101	98-99	7.160489431318753
1101	100-101	6.52419971001326
1102	1	7.917439341741286
1102	2	9.285158274161724
1102	3	8.724896267452642
1102	4	10.590711235570076
1102	5	11.349148191583286
1102	6	10.568298979133651
1102	7	10.270952821747393
1102	8	9.612242964867779
1102	9	9.565457396521278
1102	10-11	8.741257831283985
1102	12-13	8.922258291287424
1102	14-15	9.524803552273568
1102	16-17	10.211699013075009
1102	18-19	11.414512988054295
1102	20-21	11.566922429896533
1102	22-23	11.614581874717974
1102	24-25	11.44350327839118
1102	26-27	11.696384631966904
1102	28-29	11.395079246455023
1102	30-31	11.382804293755061
1102	32-33	11.354291059410041
1102	34-35	11.060788726635565
1102	36-37	11.379622117299522
1102	38-39	11.330175464869821
1102	40-41	11.012501723315324
1102	42-43	10.738901272839207
1102	44-45	10.69817976460892
1102	46-47	10.72127986051127
1102	48-49	10.389929118119134
1102	50-51	12.179962893342616
1102	52-53	11.379599194825008
1102	54-55	11.086712235369202
1102	56-57	10.76133102428836
1102	58-59	10.81750063627894
1102	60-61	10.815018278655312
1102	62-63	10.76223734300531
1102	64-65	10.414553965556838
1102	66-67	10.207772430404212
1102	68-69	10.182844991536903
1102	70-71	9.775006945214809
1102	72-73	9.475655873576574
1102	74-75	9.183477124328725
1102	76-77	9.267016654596333
1102	78-79	8.917330441416363
1102	80-81	8.531978812170848
1102	82-83	8.319872822805308
1102	84-85	8.23180473657232
1102	86-87	8.090536515213941
1102	88-89	7.8377042083032915
1102	90-91	7.406023535871265
1102	92-93	7.088432540215662
1102	94-95	6.896690222159387
1102	96-97	6.869840300556568
1102	98-99	6.5521717912347075
1102	100-101	6.060842575251842
1103	1	7.999097580365699
1103	2	9.484339964112067
1103	3	9.007617303232323
1103	4	10.97904990533744
1103	5	11.797705308476761
1103	6	10.829043738459909
1103	7	10.479388024708065
1103	8	10.002421255185705
1103	9	9.713515877927865
1103	10-11	9.244762316957445
1103	12-13	9.186589372975217
1103	14-15	9.695805432244143
1103	16-17	10.557887526159217
1103	18-19	11.467070611880597
1103	20-21	12.007336085939198
1103	22-23	12.238344378966094
1103	24-25	12.062055371421305
1103	26-27	12.021626789000134
1103	28-29	11.73235864259119
1103	30-31	11.374196665439111
1103	32-33	11.638179972925709
1103	34-35	11.505819762062899
1103	36-37	11.731317131285635
1103	38-39	11.62076063697124
1103	40-41	11.261962396808535
1103	42-43	10.853229541390846
1103	44-45	10.972215171479338
1103	46-47	10.901545056845439
1103	48-49	11.000724201325454
1103	50-51	12.663781819440679
1103	52-53	11.632736694100416
1103	54-55	11.366591577402229
1103	56-57	11.05578297990105
1103	58-59	11.015092478070436
1103	60-61	10.789010124538645
1103	62-63	10.72791310377394
1103	64-65	10.323855614452444
1103	66-67	10.20037544517687
1103	68-69	10.270005752890892
1103	70-71	9.928476309309644
1103	72-73	9.748911700746586
1103	74-75	9.666969414062422
1103	76-77	9.36869731874652
1103	78-79	9.05082099780355
1103	80-81	8.722223537966798
1103	82-83	8.432812567133851
1103	84-85	8.26541588841234
1103	86-87	8.02137570498526
1103	88-89	7.822369064333232
1103	90-91	7.644566399541569
1103	92-93	7.41901425699025
1103	94-95	7.010057620014132
1103	96-97	6.563966301777299
1103	98-99	6.017215164679387
1103	100-101	5.65096920616042
1104	1	8.014832505067915
1104	2	9.537805212568038
1104	3	8.775955826927262
1104	4	10.915383350695656
1104	5	11.527257014020538
1104	6	10.806078452087107
1104	7	10.247125895450012
1104	8	9.70282233409441
1104	9	9.643406820170885
1104	10-11	9.097336016221302
1104	12-13	8.895693553592526
1104	14-15	9.417283791248522
1104	16-17	10.52374076256351
1104	18-19	11.315089534477377
1104	20-21	11.554421792805446
1104	22-23	11.831179189470095
1104	24-25	11.517607892386795
1104	26-27	11.571319662678885
1104	28-29	11.559992022385469
1104	30-31	11.422884043556099
1104	32-33	11.487523186274895
1104	34-35	11.470667502905428
1104	36-37	11.385812472272669
1104	38-39	10.921567020206574
1104	40-41	10.80108577929197
1104	42-43	10.290426748921266
1104	44-45	10.39422298094713
1104	46-47	10.345839287526953
1104	48-49	10.283181287449263
1104	50-51	11.874220714440721
1104	52-53	11.057078557274668
1104	54-55	10.652585002539997
1104	56-57	10.496291124184243
1104	58-59	10.44231657996625
1104	60-61	10.432071512585637
1104	62-63	10.419411912681792
1104	64-65	10.320096041852068
1104	66-67	10.091471734056661
1104	68-69	9.880916117785112
1104	70-71	9.577757666457916
1104	72-73	9.38775382710001
1104	74-75	9.242097624715456
1104	76-77	8.993953117433062
1104	78-79	8.729369320494518
1104	80-81	8.779897414614254
1104	82-83	8.414548897696974
1104	84-85	7.882076529427829
1104	86-87	7.501107550449138
1104	88-89	7.297752786848081
1104	90-91	7.054056058600709
1104	92-93	6.773527031324113
1104	94-95	6.3213021846855515
1104	96-97	6.106495832831463
1104	98-99	5.678653343037356
1104	100-101	5.109680157072539
1105	1	7.881851485875188
1105	2	9.132679861400536
1105	3	8.61679043860736
1105	4	10.642897959577454
1105	5	10.964537792153589
1105	6	10.446958174668701
1105	7	10.210225505311627
1105	8	9.656285557186497
1105	9	9.53906222306728
1105	10-11	8.888680573368749
1105	12-13	8.829522897743022
1105	14-15	9.644353359540744
1105	16-17	10.524623411626198
1105	18-19	11.0212231033838
1105	20-21	11.491345283871023
1105	22-23	11.792797762615553
1105	24-25	11.791326469364225
1105	26-27	11.878386214486401
1105	28-29	11.570695429254027
1105	30-31	11.443496575413622
1105	32-33	11.270894718868686
1105	34-35	11.494130285525287
1105	36-37	11.395468291640327
1105	38-39	11.385744014219558
1105	40-41	10.90423373745774
1105	42-43	10.83921948001134
1105	44-45	10.777061119405143
1105	46-47	10.5596184587546
1105	48-49	10.369466523352141
1105	50-51	12.089242276442508
1105	52-53	11.15938464074156
1105	54-55	10.923617108055865
1105	56-57	10.789197312191696
1105	58-59	10.825124785404679
1105	60-61	10.686057479386033
1105	62-63	10.721041291715505
1105	64-65	10.214011715831166
1105	66-67	9.948987356160949
1105	68-69	9.769615264209943
1105	70-71	9.764695919479617
1105	72-73	9.496439666627115
1105	74-75	9.236981737564168
1105	76-77	8.969017071136292
1105	78-79	8.803331084505318
1105	80-81	8.550630053565172
1105	82-83	8.414472121888199
1105	84-85	8.079120892617498
1105	86-87	7.877674209920059
1105	88-89	7.700325853757997
1105	90-91	7.2317829873678985
1105	92-93	6.889724354153023
1105	94-95	6.4525609983915775
1105	96-97	5.823923832327985
1105	98-99	5.349267463595011
1105	100-101	4.526123803424344
1106	1	7.927640570450404
1106	2	9.258735509934084
1106	3	8.824979039773577
1106	4	10.512373671651453
1106	5	11.213710601913274
1106	6	10.351078657609172
1106	7	9.458275780949833
1106	8	8.960260107935138
1106	9	8.93530987785152
1106	10-11	8.478670920346996
1106	12-13	8.280973296989519
1106	14-15	9.014748554664763
1106	16-17	10.221022855182209
1106	18-19	11.254070799922001
1106	20-21	11.368733304340115
1106	22-23	11.454835893774007
1106	24-25	11.139804275337799
1106	26-27	11.577060973798194
1106	28-29	11.482567750299335
1106	30-31	10.961513413425447
1106	32-33	11.304053778756693
1106	34-35	10.92185078748242
1106	36-37	11.167648641229917
1106	38-39	10.713528111744708
1106	40-41	10.732087493833664
1106	42-43	10.555703134296738
1106	44-45	9.894571811108573
1106	46-47	9.904771380946837
1106	48-49	10.139394776802874
1106	50-51	11.874657187570627
1106	52-53	11.1031920653691
1106	54-55	10.6093008017526
1106	56-57	10.199451356849153
1106	58-59	10.130411278960029
1106	60-61	10.112610523808534
1106	62-63	10.086861188791278
1106	64-65	9.933920849270926
1106	66-67	9.67217941270933
1106	68-69	9.37134791615891
1106	70-71	9.102399065289855
1106	72-73	8.900116014105585
1106	74-75	8.471178996822559
1106	76-77	8.250916518481851
1106	78-79	7.912582069272011
1106	80-81	7.7977993617790595
1106	82-83	7.595885404198089
1106	84-85	7.210913922460557
1106	86-87	7.1496010040488756
1106	88-89	6.861420317636332
1106	90-91	6.59865128115324
1106	92-93	6.291759067824833
1106	94-95	5.889636181030744
1106	96-97	5.439209023655565
1106	98-99	5.0024394217390675
1106	100-101	4.346123817204532
1107	1	7.339161727484228
1107	2	8.495825438712384
1107	3	8.37704009735204
1107	4	10.04047254412636
1107	5	10.998930733427503
1107	6	9.992203268257818
1107	7	9.959687464222753
1107	8	8.745152364624698
1107	9	5.946455800363768
1107	10-11	7.869492246782816
1107	12-13	7.771381136840883
1107	14-15	8.50012351595732
1107	16-17	9.498179998299566
1107	18-19	10.763968976573675
1107	20-21	11.060071033624798
1107	22-23	10.65287684169655
1107	24-25	10.701077704726305
1107	26-27	10.60807428915913
1107	28-29	10.761701249998783
1107	30-31	10.570078232456755
1107	32-33	11.246462565801988
1107	34-35	11.252708362301632
1107	36-37	11.314155863583697
1107	38-39	10.917094661226482
1107	40-41	10.637741513032555
1107	42-43	10.493028950796127
1107	44-45	9.88498693706715
1107	46-47	10.219416456449121
1107	48-49	10.352457856276086
1107	50-51	11.964772672369985
1107	52-53	10.880439374063247
1107	54-55	10.748921742024738
1107	56-57	10.34579008797358
1107	58-59	10.444578203676326
1107	60-61	10.471167145969229
1107	62-63	10.434658962885525
1107	64-65	10.2902916392671
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2112	52-53	-16.70693926671345
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2112	76-77	-13.802742018103515
2112	78-79	-13.44070123279178
2112	80-81	-13.013270056607432
2112	82-83	-12.559086453212792
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2112	86-87	-11.685858658240058
2112	88-89	-11.262031839962168
2112	90-91	-10.797596373631
2112	92-93	-10.229629299905001
2112	94-95	-9.664772824597774
2112	96-97	-9.02495607953393
2112	98-99	-8.335271647679319
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2113	1	-19.222078003658414
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2113	9	-22.46093777693272
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2114	78-79	-13.44070123279178
2114	80-81	-13.013270056607432
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2114	86-87	-11.685858658240058
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2114	90-91	-10.797596373631
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2114	98-99	-8.335271647679319
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2115	8	-21.12116578324685
2115	9	-22.46093777693272
2115	10-11	-22.85828968002824
2115	12-13	-22.810396309014234
2115	14-15	-22.80570172676113
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2115	18-19	-20.591520194449476
2115	20-21	-20.254156001476023
2115	22-23	-19.902574988027123
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2115	26-27	-19.45727298492601
2115	28-29	-19.28516208084513
2115	30-31	-19.28951848150889
2115	32-33	-19.175120705295843
2115	34-35	-18.883027261298068
2115	36-37	-18.4210943231228
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2115	40-41	-18.20318455119448
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2115	46-47	-17.316804604043785
2115	48-49	-17.084995467099567
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2115	52-53	-16.70693926671345
2115	54-55	-17.513400886802746
2115	56-57	-17.500923877672424
2115	58-59	-17.228125306405826
2115	60-61	-16.9478022341652
2115	62-63	-16.536028117024856
2115	64-65	-16.192157949978604
2115	66-67	-15.858026966277539
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2115	78-79	-13.44070123279178
2115	80-81	-13.013270056607432
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2115	86-87	-11.685858658240058
2115	88-89	-11.262031839962168
2115	90-91	-10.797596373631
2115	92-93	-10.229629299905001
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2115	96-97	-9.02495607953393
2115	98-99	-8.335271647679319
2115	100-101	-7.341118209061884
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2116	2	-17.90073916173571
2116	3	-18.60428925290935
2116	4	-20.08387398313279
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2116	9	-22.46093777693272
2116	10-11	-22.85828968002824
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2116	36-37	-18.4210943231228
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2116	48-49	-17.084995467099567
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2116	54-55	-17.513400886802746
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2116	76-77	-13.802742018103515
2116	78-79	-13.44070123279178
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2116	98-99	-8.335271647679319
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2201	1	8.642310485550219
2201	2	9.745304003731913
2201	3	9.302545279464752
2201	4	11.625118822622603
2201	5	12.197751661748331
2201	6	11.261126979177057
2201	7	11.123459956311237
2201	8	10.550416950566099
2201	9	10.425752870549292
2201	10-11	10.012393773209165
2201	12-13	9.68079074134548
2201	14-15	10.47415438834679
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2201	26-27	12.840029173347371
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2201	64-65	10.816115431316359
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2201	68-69	10.354707682379704
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2201	74-75	9.89231394627388
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2201	94-95	7.783608470366255
2201	96-97	7.333497157876145
2201	98-99	6.783073676061688
2201	100-101	5.630284668635957
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2202	2	9.887161450435698
2202	3	9.10438917705104
2202	4	11.67089735284128
2202	5	11.87432347595703
2202	6	10.930573719228299
2202	7	10.617257931974635
2202	8	10.312395613944364
2202	9	10.204888654468075
2202	10-11	9.449056376867699
2202	12-13	9.475343698187782
2202	14-15	9.831136587966995
2202	16-17	10.864298799838831
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2202	20-21	12.1648213121718
2202	22-23	12.39180743905246
2202	24-25	12.339732457855543
2202	26-27	12.461524278307689
2202	28-29	12.349335578499488
2202	30-31	11.877928403618942
2202	32-33	11.89581915786584
2202	34-35	12.103829058471462
2202	36-37	12.069543055343168
2202	38-39	12.10135494716106
2202	40-41	11.702288981394648
2202	42-43	11.26886221846507
2202	44-45	10.844244984137358
2202	46-47	11.125759313853226
2202	48-49	11.182559448276741
2202	50-51	12.72928387516323
2202	52-53	11.773435238147908
2202	54-55	11.413858745894409
2202	56-57	11.393026428413279
2202	58-59	10.865320858520352
2202	60-61	10.874127906274126
2202	62-63	10.664367993886199
2202	64-65	10.536866176776886
2202	66-67	10.514677390942484
2202	68-69	10.567627594919244
2202	70-71	10.208291341768923
2202	72-73	10.033653832462079
2202	74-75	9.51361164344204
2202	76-77	9.332115742069334
2202	78-79	9.067581086257556
2202	80-81	8.880140098235927
2202	82-83	8.646171018879393
2202	84-85	8.31435778355217
2202	86-87	8.147054557460336
2202	88-89	7.888130205410535
2202	90-91	7.62408169622856
2202	92-93	7.46770595396608
2202	94-95	6.99169098526899
2202	96-97	6.580913563966249
2202	98-99	5.971174157146033
2202	100-101	4.883944808583058
2203	1	8.734983742260678
2203	2	10.27314302421177
2203	3	9.60224020060378
2203	4	11.609880452637263
2203	5	12.174932018243826
2203	6	11.12840439925661
2203	7	10.990088158362795
2203	8	10.374221015901487
2203	9	10.204427730519374
2203	10-11	9.78773587000725
2203	12-13	9.714266572461991
2203	14-15	10.173361438604381
2203	16-17	11.311627344947144
2203	18-19	12.416819053243923
2203	20-21	12.777249250475716
2203	22-23	12.85594169046826
2203	24-25	12.62604130424737
2203	26-27	12.588326731184704
2203	28-29	12.392091290340108
2203	30-31	12.12442757952731
2203	32-33	12.211501012234319
2203	34-35	12.035354569787813
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2312	86-87	-11.685858658240058
2312	88-89	-11.262031839962168
2312	90-91	-10.797596373631
2312	92-93	-10.229629299905001
2312	94-95	-9.664772824597774
2312	96-97	-9.02495607953393
2312	98-99	-8.335271647679319
2312	100-101	-7.341118209061884
2313	1	-19.222078003658414
2313	2	-17.90073916173571
2313	3	-18.60428925290935
2313	4	-20.08387398313279
2313	5	-19.415557690769656
2313	6	-20.423405394923662
2313	7	-20.63553284944416
2313	8	-21.12116578324685
2313	9	-22.46093777693272
2313	10-11	-22.85828968002824
2313	12-13	-22.810396309014234
2313	14-15	-22.80570172676113
2313	16-17	-21.646144124179894
2313	18-19	-20.591520194449476
2313	20-21	-20.254156001476023
2313	22-23	-19.902574988027123
2313	24-25	-19.737869270628433
2313	26-27	-19.45727298492601
2313	28-29	-19.28516208084513
2313	30-31	-19.28951848150889
2313	32-33	-19.175120705295843
2313	34-35	-18.883027261298068
2313	36-37	-18.4210943231228
2313	38-39	-18.313301156547976
2313	40-41	-18.20318455119448
2313	42-43	-18.04748635913666
2313	44-45	-17.656835318347337
2313	46-47	-17.316804604043785
2313	48-49	-17.084995467099567
2313	50-51	-14.792322174530685
2313	52-53	-16.70693926671345
2313	54-55	-17.513400886802746
2313	56-57	-17.500923877672424
2313	58-59	-17.228125306405826
2313	60-61	-16.9478022341652
2313	62-63	-16.536028117024856
2313	64-65	-16.192157949978604
2313	66-67	-15.858026966277539
2313	68-69	-15.404824691721014
2313	70-71	-15.071146900939038
2313	72-73	-14.663861471823122
2313	74-75	-14.22225439904986
2313	76-77	-13.802742018103515
2313	78-79	-13.44070123279178
2313	80-81	-13.013270056607432
2313	82-83	-12.559086453212792
2313	84-85	-12.166196771723305
2313	86-87	-11.685858658240058
2313	88-89	-11.262031839962168
2313	90-91	-10.797596373631
2313	92-93	-10.229629299905001
2313	94-95	-9.664772824597774
2313	96-97	-9.02495607953393
2313	98-99	-8.335271647679319
2313	100-101	-7.341118209061884
2314	1	-19.222078003658414
2314	2	-17.90073916173571
2314	3	-18.60428925290935
2314	4	-20.08387398313279
2314	5	-19.415557690769656
2314	6	-20.423405394923662
2314	7	-20.63553284944416
2314	8	-21.12116578324685
2314	9	-22.46093777693272
2314	10-11	-22.85828968002824
2314	12-13	-22.810396309014234
2314	14-15	-22.80570172676113
2314	16-17	-21.646144124179894
2314	18-19	-20.591520194449476
2314	20-21	-20.254156001476023
2314	22-23	-19.902574988027123
2314	24-25	-19.737869270628433
2314	26-27	-19.45727298492601
2314	28-29	-19.28516208084513
2314	30-31	-19.28951848150889
2314	32-33	-19.175120705295843
2314	34-35	-18.883027261298068
2314	36-37	-18.4210943231228
2314	38-39	-18.313301156547976
2314	40-41	-18.20318455119448
2314	42-43	-18.04748635913666
2314	44-45	-17.656835318347337
2314	46-47	-17.316804604043785
2314	48-49	-17.084995467099567
2314	50-51	-14.792322174530685
2314	52-53	-16.70693926671345
2314	54-55	-17.513400886802746
2314	56-57	-17.500923877672424
2314	58-59	-17.228125306405826
2314	60-61	-16.9478022341652
2314	62-63	-16.536028117024856
2314	64-65	-16.192157949978604
2314	66-67	-15.858026966277539
2314	68-69	-15.404824691721014
2314	70-71	-15.071146900939038
2314	72-73	-14.663861471823122
2314	74-75	-14.22225439904986
2314	76-77	-13.802742018103515
2314	78-79	-13.44070123279178
2314	80-81	-13.013270056607432
2314	82-83	-12.559086453212792
2314	84-85	-12.166196771723305
2314	86-87	-11.685858658240058
2314	88-89	-11.262031839962168
2314	90-91	-10.797596373631
2314	92-93	-10.229629299905001
2314	94-95	-9.664772824597774
2314	96-97	-9.02495607953393
2314	98-99	-8.335271647679319
2314	100-101	-7.341118209061884
2315	1	-19.222078003658414
2315	2	-17.90073916173571
2315	3	-18.60428925290935
2315	4	-20.08387398313279
2315	5	-19.415557690769656
2315	6	-20.423405394923662
2315	7	-20.63553284944416
2315	8	-21.12116578324685
2315	9	-22.46093777693272
2315	10-11	-22.85828968002824
2315	12-13	-22.810396309014234
2315	14-15	-22.80570172676113
2315	16-17	-21.646144124179894
2315	18-19	-20.591520194449476
2315	20-21	-20.254156001476023
2315	22-23	-19.902574988027123
2315	24-25	-19.737869270628433
2315	26-27	-19.45727298492601
2315	28-29	-19.28516208084513
2315	30-31	-19.28951848150889
2315	32-33	-19.175120705295843
2315	34-35	-18.883027261298068
2315	36-37	-18.4210943231228
2315	38-39	-18.313301156547976
2315	40-41	-18.20318455119448
2315	42-43	-18.04748635913666
2315	44-45	-17.656835318347337
2315	46-47	-17.316804604043785
2315	48-49	-17.084995467099567
2315	50-51	-14.792322174530685
2315	52-53	-16.70693926671345
2315	54-55	-17.513400886802746
2315	56-57	-17.500923877672424
2315	58-59	-17.228125306405826
2315	60-61	-16.9478022341652
2315	62-63	-16.536028117024856
2315	64-65	-16.192157949978604
2315	66-67	-15.858026966277539
2315	68-69	-15.404824691721014
2315	70-71	-15.071146900939038
2315	72-73	-14.663861471823122
2315	74-75	-14.22225439904986
2315	76-77	-13.802742018103515
2315	78-79	-13.44070123279178
2315	80-81	-13.013270056607432
2315	82-83	-12.559086453212792
2315	84-85	-12.166196771723305
2315	86-87	-11.685858658240058
2315	88-89	-11.262031839962168
2315	90-91	-10.797596373631
2315	92-93	-10.229629299905001
2315	94-95	-9.664772824597774
2315	96-97	-9.02495607953393
2315	98-99	-8.335271647679319
2315	100-101	-7.341118209061884
2316	1	-19.222078003658414
2316	2	-17.90073916173571
2316	3	-18.60428925290935
2316	4	-20.08387398313279
2316	5	-19.415557690769656
2316	6	-20.423405394923662
2316	7	-20.63553284944416
2316	8	-21.12116578324685
2316	9	-22.46093777693272
2316	10-11	-22.85828968002824
2316	12-13	-22.810396309014234
2316	14-15	-22.80570172676113
2316	16-17	-21.646144124179894
2316	18-19	-20.591520194449476
2316	20-21	-20.254156001476023
2316	22-23	-19.902574988027123
2316	24-25	-19.737869270628433
2316	26-27	-19.45727298492601
2316	28-29	-19.28516208084513
2316	30-31	-19.28951848150889
2316	32-33	-19.175120705295843
2316	34-35	-18.883027261298068
2316	36-37	-18.4210943231228
2316	38-39	-18.313301156547976
2316	40-41	-18.20318455119448
2316	42-43	-18.04748635913666
2316	44-45	-17.656835318347337
2316	46-47	-17.316804604043785
2316	48-49	-17.084995467099567
2316	50-51	-14.792322174530685
2316	52-53	-16.70693926671345
2316	54-55	-17.513400886802746
2316	56-57	-17.500923877672424
2316	58-59	-17.228125306405826
2316	60-61	-16.9478022341652
2316	62-63	-16.536028117024856
2316	64-65	-16.192157949978604
2316	66-67	-15.858026966277539
2316	68-69	-15.404824691721014
2316	70-71	-15.071146900939038
2316	72-73	-14.663861471823122
2316	74-75	-14.22225439904986
2316	76-77	-13.802742018103515
2316	78-79	-13.44070123279178
2316	80-81	-13.013270056607432
2316	82-83	-12.559086453212792
2316	84-85	-12.166196771723305
2316	86-87	-11.685858658240058
2316	88-89	-11.262031839962168
2316	90-91	-10.797596373631
2316	92-93	-10.229629299905001
2316	94-95	-9.664772824597774
2316	96-97	-9.02495607953393
2316	98-99	-8.335271647679319
2316	100-101	-7.341118209061884
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
2	615141.0
3	22389.0
4	92483.0
5	45778.0
6	25783.0
7	25858.0
8	24996.0
9	23941.0
10	25971.0
11	28084.0
12	29966.0
13	31055.0
14	32575.0
15	34246.0
16	35396.0
17	35774.0
18	35180.0
19	35271.0
20	37291.0
21	39951.0
22	42773.0
23	46042.0
24	49800.0
25	53601.0
26	58734.0
27	63643.0
28	68742.0
29	75445.0
30	83616.0
31	90846.0
32	100718.0
33	110363.0
34	118328.0
35	118051.0
36	96541.0
37	47745.0
38	7621.0
39	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.98555995244643	20.841618835853197	14.264392356633463	22.908428855066912
2	14.623510700633387	21.040598230977427	42.306314350288794	22.029576718100394
3	18.385304740499073	26.908916838900282	30.584375295330997	24.12140312526965
4	11.882938732551338	17.89532842058572	40.456873237815756	29.76485960904719
5	12.222000592567708	38.445746227046754	36.18547369256001	13.146779487825533
6	28.425239934719393	39.50320889107922	18.68311732305194	13.388433851149456
7	25.223693429085163	33.58202317787964	23.840364837572277	17.353918555462915
8	23.819867735881655	37.21216663556181	21.22151492364789	17.746450704908643
9	25.031649019803954	17.169401772705527	22.077139811628637	35.72180939586189
10-11	23.258131145633325	27.771013162611652	29.579417676901333	19.391438014853687
12-13	24.113589670743917	25.472493298771514	29.80952226532659	20.60439476515798
14-15	21.365190409305335	27.161818904421363	27.50973214087351	23.96325854539979
16-17	20.50451789236448	30.171823530756843	28.34205721147806	20.981601365400618
18-19	20.298813636688134	28.92561000144942	30.5992605780817	20.176315783780744
20-21	20.985880319214022	28.153845897138684	30.725128426467087	20.1351453571802
22-23	20.760415468723735	28.080325943684148	30.321105837704902	20.83815274988721
24-25	20.569449052119857	28.34972581620904	30.620252739127512	20.46057239254359
26-27	20.512656754480695	28.484844463392207	30.46324512118181	20.539253660945292
28-29	20.448334134445034	28.559928507747866	30.466008136039118	20.52572922176798
30-31	20.696311116042967	28.26264096588968	30.499655132648822	20.54139278541853
32-33	20.470430186912424	28.57285911954481	30.302004431861075	20.654706261681692
34-35	20.758097365533505	28.463250130973222	30.427285577655404	20.35136692583787
36-37	20.81277201501373	28.34203872105651	30.25005647841436	20.595132785515396
38-39	20.633648401852625	28.555998201789436	30.320403719536948	20.489949676820995
40-41	20.821301045097673	28.47016438400717	30.292672461049484	20.41586210984568
42-43	20.727472145142265	28.470136113303298	30.062694644569472	20.739697096984973
44-45	20.672277677187747	28.874154043632306	29.86399474032988	20.589573538850072
46-47	20.871728771296077	28.748402411321067	29.524092113662565	20.855776703720288
48-49	20.806604009430636	28.970437596392873	29.386277885824686	20.836680508351805
50-51	21.190093033977938	29.09646090544079	28.930062264049766	20.783383796531503
52-53	20.63466406556086	29.30316720495482	29.138559634275317	20.923609095209
54-55	20.571409884222458	29.23536146169855	29.186333295738205	21.00689535834079
56-57	20.63357439544221	29.299561887231395	29.216891613583535	20.84997210374286
58-59	20.558772236700563	29.224943626188892	29.32444454926501	20.89183958784553
60-61	20.616195536618832	29.34922288485129	29.2332997025854	20.80128187594448
62-63	20.51116215968209	29.235294775169805	29.270232169905956	20.98331089524215
64-65	20.342498317596448	29.22311363452504	29.264838773806616	21.169549274071898
66-67	20.322692979153643	29.26042613121283	29.141240262378403	21.275640627255118
68-69	20.43089383112611	29.23660517157894	29.144261120163765	21.18823987713118
70-71	20.556213135513325	29.11223133592039	29.102357005276257	21.229198523290023
72-73	20.477599275316738	29.269375332500907	29.0332471621708	21.219778230011556
74-75	20.440144069198173	29.479123251233542	28.744210625164985	21.3365220544033
76-77	20.416299425584295	29.38787243311147	28.895389478163086	21.30043866314115
78-79	20.28875296192208	29.338483195319036	28.878777859601136	21.49398598315775
80-81	20.352298364890963	29.441377927677575	28.712824300009387	21.49349940742207
82-83	20.531375637215675	29.264425629690745	28.857233178313734	21.346965554779846
84-85	20.52559030176454	29.388643947778352	28.634498109201722	21.451267641255388
86-87	20.38812764127112	29.404698294510585	28.75160228620097	21.455571778017326
88-89	20.49135984065346	29.30036599598409	28.613469297141364	21.594804866221086
90-91	20.43591595662326	29.283551170549543	28.713956601623696	21.5665762712035
92-93	20.414607168650466	29.207067374608236	28.618505207556392	21.759820249184898
94-95	20.172624093153935	29.194623193769576	28.758212851645354	21.874539861431135
96-97	20.25584324400441	29.063358786491296	28.686001777125327	21.994796192378967
98-99	20.073459588704218	29.119945848814954	28.47699792063345	22.329596641847374
100-101	20.28795866830364	29.241568641683337	28.425111531098825	22.045361158914197
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	147971.0
1	134034.5
2	88756.5
3	45001.0
4	28000.0
5	22042.0
6	20743.0
7	21143.5
8	21775.5
9	21325.0
10	19286.5
11	16152.0
12	12737.5
13	9887.0
14	7648.0
15	6445.0
16	6305.5
17	6897.0
18	7891.5
19	9165.5
20	10683.0
21	12334.5
22	14124.5
23	16015.0
24	18185.5
25	20769.5
26	23679.5
27	26963.5
28	30810.5
29	35446.0
30	40429.0
31	45367.5
32	50843.0
33	55916.0
34	59989.0
35	63705.0
36	67663.0
37	71841.5
38	74504.0
39	75581.5
40	75284.5
41	74587.0
42	74231.5
43	73919.0
44	73713.0
45	73331.0
46	71913.5
47	69636.0
48	67058.0
49	63743.0
50	59528.5
51	54332.5
52	49350.0
53	44544.5
54	39540.5
55	33881.0
56	28120.5
57	22841.0
58	18093.0
59	14077.0
60	10479.5
61	7696.5
62	5556.0
63	3773.5
64	2442.0
65	1581.5
66	996.0
67	584.5
68	341.0
69	201.0
70	128.5
71	84.5
72	47.0
73	30.5
74	23.5
75	16.5
76	12.0
77	9.5
78	8.0
79	6.5
80	3.5
81	2.0
82	2.0
83	2.5
84	1.0
85	0.0
86	0.0
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	21.67658500901257
2	28.602677155566035
3	22.426237973279445
4	28.678897518076933
5	31.685677265533734
6	24.488729904997474
7	23.396782568367236
8	19.514444814545058
9	11.561350020798237
10-11	15.086292765878778
12-13	15.222019198128317
14-15	19.29705940008638
16-17	22.461001311643667
18-19	26.329134904862006
20-21	28.364971623553487
22-23	27.937053853889587
24-25	26.49902702495948
26-27	27.94402644324631
28-29	24.94145017108742
30-31	26.75243084386659
32-33	25.79479549993067
34-35	23.90946710292498
36-37	26.808331088823618
38-39	26.92545066833265
40-41	24.04543259538104
42-43	24.875808222714863
44-45	23.641381003001
46-47	26.817594671826118
48-49	22.304337189640087
50-51	27.92697348184816
52-53	21.49087507191728
54-55	22.63449925652276
56-57	24.694500977756245
58-59	23.977957852091855
60-61	23.525655941363315
62-63	23.36365280808087
64-65	21.93686180885702
66-67	22.426596563589218
68-69	23.393116978533985
70-71	21.92188070258201
72-73	21.773902434748514
74-75	19.173405508265905
76-77	21.342020249993226
78-79	20.46586459311156
80-81	21.500397636810174
82-83	22.298121624270667
84-85	19.340707809459452
86-87	20.846587893672393
88-89	19.68642871144955
90-91	20.81614756070934
92-93	20.805708598358137
94-95	19.904312168005138
96-97	18.79431578578293
98-99	18.181245886172277
100-101	19.857675506050615
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2509828.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.35464163672812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.46627395489605	63.427025396245575
2	8.772679314074727	12.87033494555779
3	2.3443356729756326	5.1590370950197615
4	0.9320200335886761	2.7347198224859457
5	0.4587882777831383	1.682712485195689
6	0.25738319186261577	1.1328151081439648
7	0.16753920783430912	0.8602844985550939
8	0.11254870396343246	0.6604775876732633
9	0.08422125288070752	0.55602178409345
>10	0.38414186568795894	4.7683799629425225
>50	0.013761859358082175	0.6890126343210196
>100	0.0059756374832044485	0.7454364289686395
>500	1.1034253714201192E-4	0.04512481527655958
>1k	1.6551380571082816E-4	0.23303021198120547
>5k	0.0	0.0
>10k+	5.517126857027605E-5	4.435587223539444
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	109600	4.366833105694893	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2578	0.10271620206643642	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.984336775269062E-5	0.0
4	0.0	0.0	0.0	3.984336775269062E-5	0.0
5	0.0	0.0	0.0	3.984336775269062E-5	0.0
6	0.0	0.0	0.0	3.984336775269062E-5	0.0
7	0.0	0.0	0.0	3.984336775269062E-5	0.0
8	0.0	0.0	0.0	3.984336775269062E-5	0.0
9	0.0	0.0	0.0	7.968673550538125E-5	0.0
10-11	0.0	0.0	0.0	1.1953010325807187E-4	0.0
12-13	0.0	0.0	0.0	1.1953010325807187E-4	0.0
14-15	0.0	0.0	0.0	1.1953010325807187E-4	0.0
16-17	0.0	0.0	0.0	1.1953010325807187E-4	0.0
18-19	0.0	0.0	0.0	1.1953010325807187E-4	0.0
20-21	0.0	0.0	0.0	1.1953010325807187E-4	0.0
22-23	0.0	0.0	0.0	1.3945178713441718E-4	0.0
24-25	0.0	0.0	0.0	1.992168387634531E-4	3.984336775269062E-5
26-27	0.0	0.0	0.0	2.3906020651614374E-4	3.984336775269062E-5
28-29	0.0	0.0	0.0	5.378854646613234E-4	3.984336775269062E-5
30-31	0.0	3.984336775269062E-5	0.0	0.0018327949166237686	3.984336775269062E-5
32-33	0.0	3.984336775269062E-5	0.0	0.004880812549704601	3.984336775269062E-5
34-35	0.0	3.984336775269062E-5	0.0	0.008785462589468282	3.984336775269062E-5
36-37	0.0	5.9765051629035934E-5	0.0	0.015419383320291271	3.984336775269062E-5
38-39	0.0	7.968673550538125E-5	0.0	0.026475917871662917	3.984336775269062E-5
40-41	0.0	7.968673550538125E-5	0.0	0.03769182589404532	3.984336775269062E-5
42-43	0.0	7.968673550538125E-5	0.0	0.04966475790372886	3.984336775269062E-5
44-45	0.0	7.968673550538125E-5	0.0	0.06275330421048772	3.984336775269062E-5
46-47	0.0	7.968673550538125E-5	0.0	0.07835198268566611	3.984336775269062E-5
48-49	0.0	7.968673550538125E-5	0.0	0.099229907388076	3.984336775269062E-5
50-51	0.0	7.968673550538125E-5	0.0	0.12257812089115269	3.984336775269062E-5
52-53	0.0	7.968673550538125E-5	0.0	0.14863568340141237	3.984336775269062E-5
54-55	0.0	7.968673550538125E-5	0.0	0.17724322144784424	3.984336775269062E-5
56-57	0.0	7.968673550538125E-5	0.0	0.2125444452767281	3.984336775269062E-5
58-59	0.0	7.968673550538125E-5	0.0	0.252806168390822	3.984336775269062E-5
60-61	0.0	7.968673550538125E-5	0.0	0.29561786704108806	3.984336775269062E-5
62-63	0.0	7.968673550538125E-5	0.0	0.34151742669218765	3.984336775269062E-5
64-65	0.0	7.968673550538125E-5	0.0	0.3928157626737768	3.984336775269062E-5
66-67	0.0	7.968673550538125E-5	0.0	0.4527999528254526	3.984336775269062E-5
68-69	0.0	7.968673550538125E-5	0.0	0.5170274616427899	3.984336775269062E-5
70-71	0.0	7.968673550538125E-5	0.0	0.5882076381329716	5.9765051629035934E-5
72-73	0.0	9.960841938172656E-5	0.0	0.6656631450442023	7.968673550538125E-5
74-75	0.0	1.1953010325807187E-4	0.0	0.7462264346401427	7.968673550538125E-5
76-77	0.0	1.1953010325807187E-4	0.0	0.8364915842838633	7.968673550538125E-5
78-79	0.0	1.1953010325807187E-4	0.0	0.9434112616482085	7.968673550538125E-5
80-81	0.0	1.1953010325807187E-4	0.0	1.0578214921500597	9.960841938172656E-5
82-83	0.0	1.1953010325807187E-4	0.0	1.18117655871239	1.1953010325807187E-4
84-85	0.0	1.1953010325807187E-4	0.0	1.3085956487854946	1.1953010325807187E-4
86-87	0.0	1.1953010325807187E-4	0.0	1.44380411725425	1.1953010325807187E-4
88-89	0.0	1.1953010325807187E-4	0.0	1.5955475833403723	1.593734710107625E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2320	0.0	57.96527	1
GTATCAA	4485	0.0	46.629528	1
ATCAACG	5235	0.0	39.28562	3
TCAACGC	5320	0.0	38.749016	4
CAACGCA	5420	0.0	37.939083	5
TATCAAC	5535	0.0	37.31518	2
AACGCAG	5845	0.0	34.76018	6
ACGCAGA	6805	0.0	29.094404	7
CGCAGAG	6925	0.0	27.816685	8
GTGGTAT	1260	0.0	25.566433	1
GTACATG	4630	0.0	25.069462	1
GCAGAGT	7525	0.0	24.377354	9
TGGTATC	1275	0.0	23.256248	2
TACATGG	4910	0.0	23.219093	2
ACATGGG	4810	0.0	22.78969	3
GAGTACT	4920	0.0	22.004028	12-13
CAGAGTA	7215	0.0	21.011902	10-11
AGAGTAC	6640	0.0	20.344522	10-11
GAGTACA	3375	0.0	19.392612	1
CATGGGT	1310	0.0	19.19054	4
>>END_MODULE