FastQCFastQC Report
Sat 18 Jun 2016
SRR1931046_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931046_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2509828
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72330.2881870789552113No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68320.27220988848638233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38080.1517235444022459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37750.061.443311
GTATCAA73850.043.0573541
ATCAACG92400.033.9462433
CAACGCA94350.033.1837165
TCAACGC95300.032.963124
TATCAAC95550.032.7800142
AACGCAG100350.031.4366
ACGCAGA113050.027.7366457
CGCAGAG113550.027.489188
GCAGAGT128350.023.9128559
GTGGTAT19100.023.640791
GTACATG73650.023.2973331
TACATGG77400.022.2291072
ACATGGG73650.022.0685163
ACCGTAT2606.366463E-1021.894298
GAGTACT88700.021.31217212-13
CAGAGTA127650.020.56931510-11
TGGTATC19150.020.3516452
TATTCGC1404.897131E-420.3304169
AGAGTAC123300.020.2369810-11
Produced by FastQC (version 0.11.5)