##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931046_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2509828 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.398607793043986 31.0 31.0 34.0 30.0 34.0 2 31.55452684407059 31.0 31.0 34.0 30.0 34.0 3 31.61694626085931 31.0 31.0 34.0 30.0 34.0 4 35.38837083656729 37.0 35.0 37.0 33.0 37.0 5 35.279159368689804 37.0 35.0 37.0 33.0 37.0 6 35.31104641433596 37.0 35.0 37.0 32.0 37.0 7 35.197435840224905 37.0 35.0 37.0 32.0 37.0 8 35.19854428271579 37.0 35.0 37.0 32.0 37.0 9 36.71347120201066 39.0 35.0 39.0 32.0 39.0 10-11 36.65553456252779 39.0 35.0 39.0 32.0 39.0 12-13 36.54378148622136 38.0 35.0 39.0 32.0 39.0 14-15 37.61385839985847 39.0 36.0 41.0 32.0 41.0 16-17 37.57536472618841 39.0 36.0 41.0 32.0 41.0 18-19 37.61527383549789 39.0 36.5 41.0 32.0 41.0 20-21 37.600605499659736 39.0 36.0 41.0 32.0 41.0 22-23 37.502575475291536 39.0 36.0 41.0 32.0 41.0 24-25 37.38784251351089 39.0 36.0 41.0 32.0 41.0 26-27 37.14103117823213 39.0 36.0 40.0 31.0 41.0 28-29 36.96214800376759 39.0 36.0 40.0 31.0 41.0 30-31 36.72800028527851 38.5 35.5 40.0 30.0 41.0 32-33 36.54643644903156 38.0 35.0 40.0 30.0 41.0 34-35 36.61198137880365 38.5 35.0 40.0 30.0 41.0 36-37 36.6662341005041 39.0 35.0 40.0 30.0 41.0 38-39 36.64572393008605 39.0 35.0 40.0 30.0 41.0 40-41 36.52207701085493 39.0 35.0 40.0 30.0 41.0 42-43 36.368419668598804 39.0 35.0 40.0 30.0 41.0 44-45 36.191685844607676 39.0 35.0 40.0 30.0 41.0 46-47 35.926402327171424 38.0 35.0 40.0 29.0 41.0 48-49 35.69239784558942 38.0 35.0 40.0 28.0 41.0 50-51 35.421446409873504 38.0 34.5 40.0 27.5 41.0 52-53 35.2088589337596 38.0 34.0 40.0 27.0 41.0 54-55 35.06567780740353 38.0 34.0 40.0 26.0 41.0 56-57 34.90356789389551 38.0 34.0 40.0 26.0 41.0 58-59 34.711712714974894 38.0 34.0 40.0 26.0 41.0 60-61 34.411430384871 38.0 33.0 40.0 25.5 41.0 62-63 34.161554696178385 37.0 33.0 40.0 25.0 41.0 64-65 33.86255014287832 37.0 33.0 40.0 24.5 41.0 66-67 33.549771338912464 36.5 32.0 39.0 23.0 41.0 68-69 33.212611581351396 36.0 32.0 39.0 23.0 40.5 70-71 32.84996402143892 35.5 31.5 39.0 22.0 40.0 72-73 32.432963533756094 35.0 31.0 38.0 22.0 40.0 74-75 31.916807048132384 35.0 31.0 37.5 20.0 39.5 76-77 30.54376774025949 33.5 29.5 36.0 19.5 39.0 78-79 31.278199541960646 34.0 30.5 36.0 20.0 39.0 80-81 31.236653866320722 34.0 31.0 36.0 20.0 38.5 82-83 30.963598302353788 34.0 31.0 36.0 20.0 37.5 84-85 30.615342565307266 34.0 30.0 35.5 18.5 37.0 86-87 30.264271695112175 34.0 30.0 35.0 18.0 36.5 88-89 29.91977617589731 34.0 30.0 35.0 15.0 36.0 90-91 29.58894075609962 34.0 29.5 35.0 9.0 36.0 92-93 29.26936666576355 34.0 29.0 35.0 4.5 35.5 94-95 28.89661940977629 34.0 29.0 35.0 2.0 35.0 96-97 28.481917286762283 34.0 29.0 35.0 2.0 35.0 98-99 28.004023383275666 34.0 29.0 35.0 2.0 35.0 100-101 26.89188880672301 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2979147910754172 1101 2 0.32991993591152635 1101 3 0.28063703616308544 1101 4 0.26154551616568256 1101 5 0.23355697703095046 1101 6 0.2505774271571255 1101 7 0.3949638711544381 1101 8 0.375530460623672 1101 9 0.5023656961143317 1101 10-11 0.43171442266726245 1101 12-13 0.372889993630956 1101 14-15 0.3650650462713543 1101 16-17 0.5386929909224705 1101 18-19 0.43067285166594615 1101 20-21 0.4379284342149887 1101 22-23 0.5045235746666634 1101 24-25 0.4322962845907412 1101 26-27 0.3251851542120221 1101 28-29 0.4474773327774315 1101 30-31 0.35841487489893353 1101 32-33 0.4582396858438287 1101 34-35 0.4508036001311453 1101 36-37 0.46272059189598025 1101 38-39 0.490374357795325 1101 40-41 0.5031379245972758 1101 42-43 0.42445928717718573 1101 44-45 0.504183797879854 1101 46-47 0.429066586168112 1101 48-49 0.4350993578944937 1101 50-51 0.5615751735187189 1101 52-53 0.4777028857862504 1101 54-55 0.39026838428876687 1101 56-57 0.5124818543958085 1101 58-59 0.5012434512185919 1101 60-61 0.46687073348736874 1101 62-63 0.3698435194175289 1101 64-65 0.45770081950169583 1101 66-67 0.4325612358088762 1101 68-69 0.3470432537509325 1101 70-71 0.26549857022401824 1101 72-73 0.3886494305796262 1101 74-75 0.4055732036058508 1101 76-77 0.30177743369302235 1101 78-79 0.3523900987431716 1101 80-81 0.38123043588803185 1101 82-83 0.36406776560511744 1101 84-85 0.364491706790421 1101 86-87 0.47090236344451597 1101 88-89 0.33553036227844046 1101 90-91 0.13804043581392378 1101 92-93 0.2474801948199037 1101 94-95 0.43403382761533393 1101 96-97 0.5804676030577802 1101 98-99 0.7181574227459961 1101 100-101 1.0236525151654625 1102 1 0.29234586719639566 1102 2 0.3629267624749666 1102 3 0.33148665124255317 1102 4 0.20275075771094464 1102 5 0.28685909823514777 1102 6 0.37874375816262784 1102 7 0.2924690804413501 1102 8 0.29447482805494474 1102 9 0.37993917103565167 1102 10-11 0.3761587944577798 1102 12-13 0.39965047105447127 1102 14-15 0.48497389464841234 1102 16-17 0.45472669653455 1102 18-19 0.4520337615256693 1102 20-21 0.4552492042304195 1102 22-23 0.4267114315791005 1102 24-25 0.40111663176029566 1102 26-27 0.5221703066545018 1102 28-29 0.49184214851252506 1102 30-31 0.4805689477330404 1102 32-33 0.4956815990252821 1102 34-35 0.6049507592170968 1102 36-37 0.5704758905472289 1102 38-39 0.4342457080250597 1102 40-41 0.4234779014061374 1102 42-43 0.5196384081808603 1102 44-45 0.5027873166550805 1102 46-47 0.6296280557537202 1102 48-49 0.5971737874884795 1102 50-51 0.417555173344347 1102 52-53 0.3929501852396058 1102 54-55 0.40194023844616567 1102 56-57 0.33569282397705535 1102 58-59 0.23741421120778483 1102 60-61 0.2262232561771924 1102 62-63 0.14769511359010323 1102 64-65 0.3003132223997085 1102 66-67 0.18203028087465611 1102 68-69 0.23820185147192063 1102 70-71 0.26395605459615723 1102 72-73 0.1392143875539631 1102 74-75 0.3170824582636875 1102 76-77 0.31938155404280977 1102 78-79 0.419120846177762 1102 80-81 0.2805092003062022 1102 82-83 0.3047900038112097 1102 84-85 0.45968761085235954 1102 86-87 0.3413696299420437 1102 88-89 0.3308051685104054 1102 90-91 0.37031403889677605 1102 92-93 0.44385854162327476 1102 94-95 0.5754068997432498 1102 96-97 0.5168332208922877 1102 98-99 0.5961136385804835 1102 100-101 0.7999273737082575 1103 1 0.3185895859464303 1103 2 0.3735516083207884 1103 3 0.34058188982491444 1103 4 0.13222401359494285 1103 5 0.23934153460427865 1103 6 0.2531417754415344 1103 7 0.32715391257952575 1103 8 0.2681148898416481 1103 9 0.4081495276958478 1103 10-11 0.3808730707543475 1103 12-13 0.409512360277148 1103 14-15 0.3926096838780211 1103 16-17 0.48675524617247845 1103 18-19 0.412625275970413 1103 20-21 0.390602625060815 1103 22-23 0.29076406961884516 1103 24-25 0.3587256535731811 1103 26-27 0.47383778600494253 1103 28-29 0.4792742496353881 1103 30-31 0.5734970644321535 1103 32-33 0.5177127174259155 1103 34-35 0.48629850149063714 1103 36-37 0.4510649385251213 1103 38-39 0.48845512583658746 1103 40-41 0.5857491138735114 1103 42-43 0.5768342967965836 1103 44-45 0.6528174086872625 1103 46-47 0.7413456487094052 1103 48-49 0.5382105958121812 1103 50-51 0.4606515667333113 1103 52-53 0.5042395563587547 1103 54-55 0.5694667586478914 1103 56-57 0.6154920342530161 1103 58-59 0.6796346678792347 1103 60-61 0.5910584575465947 1103 62-63 0.35943475698673666 1103 64-65 0.3490159972317457 1103 66-67 0.3678222952051726 1103 68-69 0.4268453629023554 1103 70-71 0.36318062356119185 1103 72-73 0.3937122796909378 1103 74-75 0.5081560646815042 1103 76-77 0.4670227535868001 1103 78-79 0.5578510817651257 1103 80-81 0.4715565223710776 1103 82-83 0.6231895623477399 1103 84-85 0.5546126279869164 1103 86-87 0.5379991802608082 1103 88-89 0.4734463737374952 1103 90-91 0.5148757068194065 1103 92-93 0.258548738611001 1103 94-95 0.4496168063016661 1103 96-97 0.6067487245118564 1103 98-99 0.5094360830878202 1103 100-101 0.8000421723956883 1104 1 0.2646228839187046 1104 2 0.29796797596684854 1104 3 0.21928637109050086 1104 4 0.09129566409075807 1104 5 0.2045018444421416 1104 6 0.28344963491476705 1104 7 0.2559290366766973 1104 8 0.2489251962795862 1104 9 0.40940106290771183 1104 10-11 0.3951857645534176 1104 12-13 0.463169151685058 1104 14-15 0.49304681875364764 1104 16-17 0.5338493479087987 1104 18-19 0.4190417549884131 1104 20-21 0.4294503060199375 1104 22-23 0.3242279105349297 1104 24-25 0.23666585187680056 1104 26-27 0.3882574024657117 1104 28-29 0.45841040911106035 1104 30-31 0.4064657339376012 1104 32-33 0.4288034181205447 1104 34-35 0.4539060558663053 1104 36-37 0.41023019182213716 1104 38-39 0.46000575068075733 1104 40-41 0.4260548998138276 1104 42-43 0.37931412742167936 1104 44-45 0.5038780025795475 1104 46-47 0.35705808915130177 1104 48-49 0.38885207581657966 1104 50-51 0.3678876928813466 1104 52-53 0.16909976358332557 1104 54-55 0.2951319891388451 1104 56-57 0.44246431802984176 1104 58-59 0.43000180352998285 1104 60-61 0.28260602665805834 1104 62-63 0.16732704017032773 1104 64-65 0.164227767623089 1104 66-67 0.16499950615510528 1104 68-69 0.11817865295546426 1104 70-71 0.21225205299209193 1104 72-73 0.14170856412145127 1104 74-75 0.34530069234243754 1104 76-77 0.38676541723989644 1104 78-79 0.41973203355310673 1104 80-81 0.42915816116164507 1104 82-83 0.4263699448795819 1104 84-85 0.41868701940780895 1104 86-87 0.45983377557630334 1104 88-89 0.5919191873705394 1104 90-91 0.4861390172356792 1104 92-93 0.4693318232836212 1104 94-95 0.4731560219577595 1104 96-97 0.4846322574425521 1104 98-99 0.5889224905339603 1104 100-101 0.6658745895222289 1105 1 0.24904966795735461 1105 2 0.30308140847416 1105 3 0.4019699760733779 1105 4 0.08970696396247746 1105 5 0.23233750711897017 1105 6 0.2704653865015203 1105 7 0.31942494880807715 1105 8 0.32199803824865114 1105 9 0.40064984802940273 1105 10-11 0.33187490530393404 1105 12-13 0.37987728592516845 1105 14-15 0.4769757873572402 1105 16-17 0.4175007917371971 1105 18-19 0.5145753393590908 1105 20-21 0.45559560539717836 1105 22-23 0.48471635195737406 1105 24-25 0.5171831790040642 1105 26-27 0.4420215376353269 1105 28-29 0.36482792577775314 1105 30-31 0.3206014012918885 1105 32-33 0.478405022584937 1105 34-35 0.4698755604605154 1105 36-37 0.373527059600967 1105 38-39 0.4165992585020106 1105 40-41 0.2778505877953066 1105 42-43 0.2793060680072941 1105 44-45 0.41267904673054545 1105 46-47 0.3284699417270147 1105 48-49 0.27038898022960467 1105 50-51 0.0915958989879826 1105 52-53 0.11167977713568433 1105 54-55 0.24351062179032823 1105 56-57 0.27136751900831513 1105 58-59 0.28733516831912453 1105 60-61 0.24015207271072825 1105 62-63 0.06257722225718254 1105 64-65 -0.0017876456319001477 1105 66-67 0.045238585750894345 1105 68-69 -0.04708066177066428 1105 70-71 -0.09267589025217404 1105 72-73 -0.03328730048529849 1105 74-75 0.13901854929097013 1105 76-77 0.18517696257600846 1105 78-79 0.26561243866209594 1105 80-81 0.19178556570701843 1105 82-83 0.1427999952311474 1105 84-85 0.2988540961136579 1105 86-87 0.3334174971411308 1105 88-89 0.358064233101242 1105 90-91 0.40976195173800534 1105 92-93 0.3443052142610874 1105 94-95 0.37156362622660666 1105 96-97 0.4310157418342193 1105 98-99 0.46959904223373883 1105 100-101 0.510014038391926 1106 1 0.19036852841848173 1106 2 0.1945549859970228 1106 3 0.26165624253122743 1106 4 0.06629414880096363 1106 5 0.18020788240171726 1106 6 0.14731418821082087 1106 7 0.26389423586937255 1106 8 0.21108447378941264 1106 9 0.12971660546157437 1106 10-11 0.18312914357303356 1106 12-13 0.28074895172253633 1106 14-15 0.29158740309119224 1106 16-17 0.1986383324834904 1106 18-19 0.2523768068113199 1106 20-21 0.2762742934405864 1106 22-23 0.22812683826710156 1106 24-25 0.33177825851230835 1106 26-27 0.3475795697969062 1106 28-29 0.4375986875258775 1106 30-31 0.3755419087748706 1106 32-33 0.316060144439696 1106 34-35 0.3420539119228323 1106 36-37 0.18972229454973188 1106 38-39 0.1688298230969778 1106 40-41 0.14733932214704737 1106 42-43 0.24944564064232821 1106 44-45 0.2813685990742485 1106 46-47 0.18178197548296993 1106 48-49 0.21643306718534205 1106 50-51 0.2548744537769707 1106 52-53 0.18642220819512545 1106 54-55 0.341433671842033 1106 56-57 0.48215292771836715 1106 58-59 0.45504839433694855 1106 60-61 0.4213534846287956 1106 62-63 0.32685856133318225 1106 64-65 0.21923313558073687 1106 66-67 0.17484380403251976 1106 68-69 0.17667810501274062 1106 70-71 0.18270766405795769 1106 72-73 0.23193226269651745 1106 74-75 0.5969926962801004 1106 76-77 0.35885935266695057 1106 78-79 0.31573821732884966 1106 80-81 0.2723506085392877 1106 82-83 0.3089028684692643 1106 84-85 0.42762437279916554 1106 86-87 0.4757247415927104 1106 88-89 0.5059284072993542 1106 90-91 0.5001764529055315 1106 92-93 0.5933784621618443 1106 94-95 0.5598328277129383 1106 96-97 0.5396783814705906 1106 98-99 0.5556729219561234 1106 100-101 0.599826207538225 1107 1 0.20401905028591116 1107 2 0.2343152730696474 1107 3 0.2029593628066415 1107 4 0.17462763392819625 1107 5 0.14031280234561905 1107 6 0.21686190310543907 1107 7 0.3304437176449966 1107 8 0.23903398106641305 1107 9 0.25981733589382117 1107 10-11 0.19809754008298341 1107 12-13 0.24421914243639264 1107 14-15 0.29294626208680086 1107 16-17 0.37296755523800584 1107 18-19 0.3356515476991362 1107 20-21 0.21068748858075992 1107 22-23 0.2558914605735225 1107 24-25 0.2883184505012295 1107 26-27 0.3468272792269431 1107 28-29 0.33085721682513736 1107 30-31 0.3671282764682715 1107 32-33 0.40703994370743857 1107 34-35 0.3464592744977466 1107 36-37 0.36662351315116126 1107 38-39 0.3565135696133055 1107 40-41 0.24300044530047415 1107 42-43 0.2742584787632367 1107 44-45 0.4256171281913552 1107 46-47 0.5106886950953466 1107 48-49 0.4517861701562609 1107 50-51 0.4164019861546109 1107 52-53 0.3459770270442917 1107 54-55 0.35918445054474546 1107 56-57 0.4554211111096791 1107 58-59 0.41739561377627865 1107 60-61 0.40342419629931925 1107 62-63 0.2729532078659034 1107 64-65 0.32079236252477017 1107 66-67 0.36899670299242615 1107 68-69 0.36145742704046313 1107 70-71 0.2906349487079538 1107 72-73 0.23562449752775194 1107 74-75 0.4645513039050151 1107 76-77 0.365093528152844 1107 78-79 0.3957715512693589 1107 80-81 0.42875965059834087 1107 82-83 0.6236554140529726 1107 84-85 0.6445654828375957 1107 86-87 0.6365947027941878 1107 88-89 0.6993290156893046 1107 90-91 0.7213844894817854 1107 92-93 0.5946954260409676 1107 94-95 0.8039319916321332 1107 96-97 0.9758566360836056 1107 98-99 0.8640191577709828 1107 100-101 1.0245206464168923 1108 1 0.03931490069294341 1108 2 0.09699456058451261 1108 3 0.07755446704471325 1108 4 0.08406923366888464 1108 5 0.04813427151221816 1108 6 0.13636430017595558 1108 7 0.11176332193672778 1108 8 0.02927963425443636 1108 9 0.09844023038110805 1108 10-11 0.17012660716373063 1108 12-13 0.1407029827426598 1108 14-15 0.04046678885098487 1108 16-17 0.03780708072973482 1108 18-19 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2316 80-81 -1.3323259985506688 2316 82-83 -0.9950484497888574 2316 84-85 -1.0287620200121346 2316 86-87 -0.7777960444248713 2316 88-89 -1.099052337244931 2316 90-91 -1.065826311980416 2316 92-93 -1.1164605819775808 2316 94-95 -1.3896260446085797 2316 96-97 -1.0745040022560808 2316 98-99 -1.229476184393242 2316 100-101 -1.4764189785978452 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 10.0 4 19.0 5 44.0 6 103.0 7 247.0 8 782.0 9 1623.0 10 2813.0 11 4736.0 12 6909.0 13 8910.0 14 11071.0 15 14255.0 16 16965.0 17 20164.0 18 16834.0 19 12641.0 20 11513.0 21 12136.0 22 13295.0 23 15549.0 24 19162.0 25 24258.0 26 31066.0 27 40306.0 28 51889.0 29 65461.0 30 80914.0 31 98904.0 32 121440.0 33 147746.0 34 184898.0 35 233681.0 36 307369.0 37 426815.0 38 421830.0 39 83438.0 40 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.140589724566226 21.970391845445338 15.176709378040684 22.712309051947752 2 14.820258950792656 22.058234043892693 41.23704110427443 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31.280749119063138 19.92887560422467 22-23 20.471085951252242 28.020199074195816 31.073189370703375 20.435525603848568 24-25 20.359218212204382 28.269174796593287 31.18208279785155 20.189524193350774 26-27 20.253976830023152 28.436503700657813 31.05898918879182 20.250530280527215 28-29 20.16236995104039 28.4749847001224 31.10907378365973 20.253571565177477 30-31 20.491244945290497 28.178447756662294 31.085688943681518 20.24461835436569 32-33 20.315776280533452 28.458322548352083 30.887747922558557 20.33815324855591 34-35 20.529920738827855 28.434240682912076 30.99065805641269 20.04518052184738 36-37 20.539874396666516 28.29732656627644 30.988929024793148 20.173870012263894 38-39 20.38011468146241 28.47700523722504 31.039637679412657 20.103242401899895 40-41 20.531138081597174 28.381147275628287 30.963345200905017 20.12436944186952 42-43 20.463948644076915 28.408396741241937 30.666415625143202 20.461238989537943 44-45 20.411216785451515 28.763080262801637 30.525916069156274 20.299786882590574 46-47 20.609290074754142 28.715472034938394 30.1733770063278 20.50186088397966 48-49 20.642986262794267 28.887979481756147 29.968203735179493 20.500830520270092 50-51 20.769165823873998 28.984602991260306 29.698317149119312 20.547914035746384 52-53 20.698618487236743 29.05217550166297 29.644486035288924 20.60471997581136 54-55 20.69021376255207 29.089444414224698 29.66537970384513 20.554962119378107 56-57 20.638124754449397 29.16473745908818 29.573277878410913 20.623859908051507 58-59 20.609236439018773 29.048867313013098 29.794487657742707 20.547408590225423 60-61 20.67370925449656 29.185710256591307 29.7344488817987 20.406131607113437 62-63 20.598087581460504 29.085036428208422 29.759265305388897 20.557610684942176 64-65 20.40509562738679 29.063712117142476 29.687854779691857 20.843337475778874 66-67 20.43203923148377 29.07781687194265 29.64670054857211 20.84344334800147 68-69 20.488673866263248 29.0923815087199 29.666511890318493 20.752432734698363 70-71 20.612731604483134 28.905074186719737 29.66847762816561 20.81371658063152 72-73 20.6060577036661 29.08868658231728 29.54118258354325 20.76407313047337 74-75 20.59423396846349 29.336609130468826 29.341643081673062 20.727513819394616 76-77 20.45410760919323 29.240653834782083 29.465767145075706 20.839471410948978 78-79 20.377943243166783 29.197223241604835 29.529156799906282 20.895676715322097 80-81 20.497875151504527 29.285456442000363 29.31470149723527 20.901966909259844 82-83 20.592725876036127 29.286210051047323 29.43727617988165 20.683787893034904 84-85 20.62627399168389 29.29533418226269 29.20767877320677 20.87071305284665 86-87 20.42831221900465 29.343265753669172 29.3625300219776 20.865892005348574 88-89 20.536200410665835 29.20925012147254 29.192256914970017 21.062292552891606 90-91 20.51112144887142 29.228376952815648 29.389433502910233 20.871068095402695 92-93 20.514271994293395 29.210529724064482 29.32410393435012 20.951094347292003 94-95 20.19941743445241 29.187107694140536 29.381588402558727 21.23188646884832 96-97 20.347553766145282 29.04002366696516 29.342195828199348 21.27022673869021 98-99 20.148532090645254 29.20552723134812 29.263041132699136 21.382899545307488 100-101 20.26184662853391 29.12956585072762 29.22666413794093 21.381923382797545 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 129.0 1 102.0 2 140.0 3 210.5 4 178.0 5 174.5 6 343.5 7 781.0 8 1369.0 9 1799.0 10 1733.0 11 1516.0 12 1640.0 13 1942.5 14 2205.5 15 2462.0 16 2844.0 17 3291.0 18 3685.5 19 4120.5 20 4751.0 21 5728.0 22 7159.0 23 9193.0 24 11752.0 25 14918.0 26 19184.0 27 24359.0 28 30332.5 29 37708.0 30 45328.5 31 53501.0 32 62741.0 33 70808.5 34 77042.5 35 82683.0 36 90000.5 37 97394.0 38 101410.0 39 102989.5 40 102244.5 41 100359.0 42 100421.5 43 102009.0 44 102502.5 45 102833.5 46 101401.5 47 98169.0 48 95105.0 49 90405.5 50 84199.5 51 76750.5 52 69275.0 53 62249.5 54 55053.0 55 46669.0 56 37645.5 57 29091.0 58 22335.5 59 16946.0 60 11903.5 61 8348.5 62 5991.5 63 3798.0 64 2097.0 65 1152.5 66 603.0 67 319.5 68 152.0 69 83.0 70 45.0 71 26.0 72 17.5 73 14.0 74 9.5 75 3.0 76 2.5 77 3.5 78 3.0 79 1.5 80 0.5 81 0.5 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004542143923806731 2 0.00645462557593588 3 0.0023507586974087467 4 0.17228272216263427 5 0.001992168387634531 6 4.3827704527959685E-4 7 0.0 8 8.367107228065031E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.9843367752690617E-4 24-25 6.574155679193953E-4 26-27 0.0028488007943173795 28-29 1.593734710107625E-4 30-31 0.006514390627564917 32-33 0.021973617315608877 34-35 0.013526823352038466 36-37 8.765540905591937E-4 38-39 2.5898189039248905E-4 40-41 0.0018925599682528048 42-43 0.011275673074011446 44-45 0.007112041143855277 46-47 0.010678022557721087 48-49 0.010219823828565145 50-51 0.18979388228994176 52-53 0.11419109197921132 54-55 0.017192413185286005 56-57 0.00645462557593588 58-59 0.017570925178936565 60-61 0.12267772931053443 62-63 0.23629109245733174 64-65 0.2419886940459665 66-67 0.2521487528229026 68-69 0.24764645226684856 70-71 0.23545438173452524 72-73 0.16176407307592391 74-75 0.2718911415443608 76-77 0.21162804781841624 78-79 0.0055382281176239966 80-81 7.968673550538125E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 5.9765051629035934E-5 90-91 0.005996426846779939 92-93 0.018148654011350577 94-95 0.002689427323306617 96-97 1.992168387634531E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2509828.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.316229570453146 #Duplication Level Percentage of deduplicated Percentage of total 1 72.16840242284603 39.19915513732152 2 15.820145069940777 17.185812629135516 3 5.255126478754588 8.563159687254036 4 2.36765404324442 5.1440816222510195 5 1.297075345399224 3.5226121115439524 6 0.753430609570447 2.4554105972900913 7 0.4768530172595767 1.8130600569784092 8 0.32737792324352966 1.4225547548154993 9 0.2358760421544493 1.1530707530247855 >10 1.1780460768233603 11.368476139390731 >50 0.07958707417471332 2.970406915941536 >100 0.03814825144316724 3.6329189182751374 >500 0.0016163933291798772 0.5289333491900621 >1k 5.143069683671169E-4 0.47904903603371324 >5k 1.4694484810489055E-4 0.5612982915539968 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7233 0.2881870789552113 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6832 0.27220988848638233 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3808 0.1517235444022459 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.984336775269062E-5 0.0 24-25 0.0 0.0 0.0 1.1953010325807187E-4 0.0 26-27 0.0 0.0 0.0 1.593734710107625E-4 0.0 28-29 0.0 0.0 0.0 5.378854646613234E-4 0.0 30-31 0.0 0.0 0.0 0.002609740587801236 0.0 32-33 0.0 0.0 0.0 0.0077096916601456355 0.0 34-35 0.0 0.0 0.0 0.014622515965237459 0.0 36-37 0.0 0.0 0.0 0.026754821445931755 0.0 38-39 0.0 0.0 0.0 0.04711478236755666 0.0 40-41 0.0 0.0 0.0 0.06982550198659032 0.0 42-43 0.0 0.0 0.0 0.09311395043803798 0.0 44-45 0.0 0.0 0.0 0.12102422954879777 0.0 46-47 0.0 0.0 0.0 0.15441297172555252 0.0 48-49 0.0 0.0 0.0 0.1950332851494206 0.0 50-51 0.0 0.0 0.0 0.2397773871356922 0.0 52-53 0.0 0.0 0.0 0.28408321207668413 0.0 54-55 0.0 0.0 0.0 0.333289771251257 0.0 56-57 0.0 0.0 0.0 0.3931345096157984 0.0 58-59 0.0 0.0 0.0 0.4594338735562756 0.0 60-61 0.0 0.0 0.0 0.5341003447248178 0.0 62-63 0.0 0.0 0.0 0.6118148335264408 0.0 64-65 0.0 0.0 0.0 0.6974780741947257 0.0 66-67 0.0 0.0 0.0 0.7966880598989253 0.0 68-69 0.0 0.0 0.0 0.9095643207422979 0.0 70-71 0.0 0.0 0.0 1.0347721039051283 0.0 72-73 0.0 0.0 0.0 1.1701797892126473 0.0 74-75 0.0 0.0 0.0 1.309771028134199 0.0 76-77 0.0 0.0 0.0 1.466474993505531 0.0 78-79 0.0 0.0 0.0 1.6526232076461016 0.0 80-81 0.0 0.0 0.0 1.8565415638043723 3.984336775269062E-5 82-83 0.0 0.0 0.0 2.073687918056536 7.968673550538125E-5 84-85 0.0 0.0 0.0 2.2998986384724374 7.968673550538125E-5 86-87 0.0 0.0 0.0 2.541867410834527 7.968673550538125E-5 88-89 0.0 0.0 0.0 2.8134198837529905 7.968673550538125E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3775 0.0 61.44331 1 GTATCAA 7385 0.0 43.057354 1 ATCAACG 9240 0.0 33.946243 3 CAACGCA 9435 0.0 33.183716 5 TCAACGC 9530 0.0 32.96312 4 TATCAAC 9555 0.0 32.780014 2 AACGCAG 10035 0.0 31.436 6 ACGCAGA 11305 0.0 27.736645 7 CGCAGAG 11355 0.0 27.48918 8 GCAGAGT 12835 0.0 23.912855 9 GTGGTAT 1910 0.0 23.64079 1 GTACATG 7365 0.0 23.297333 1 TACATGG 7740 0.0 22.229107 2 ACATGGG 7365 0.0 22.068516 3 ACCGTAT 260 6.366463E-10 21.89429 8 GAGTACT 8870 0.0 21.312172 12-13 CAGAGTA 12765 0.0 20.569315 10-11 TGGTATC 1915 0.0 20.351645 2 TATTCGC 140 4.897131E-4 20.330416 9 AGAGTAC 12330 0.0 20.23698 10-11 >>END_MODULE