FastQCFastQC Report
Sat 18 Jun 2016
SRR1931045_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931045_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518804
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1135724.508965366102325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18800.052.6186451
GTATCAA38050.046.204011
ATCAACG44100.038.837283
TCAACGC44100.038.834964
CAACGCA44050.038.7577365
TATCAAC46350.037.591542
AACGCAG47450.034.8301856
ACGCAGA56750.028.76447
CGCAGAG57250.028.0637078
GTACATG39350.024.3577461
GTGGTAT11600.023.4184471
GCAGAGT66250.023.0428589
TGGTATC10800.022.3015482
TACATGG42250.021.7113722
ACATGGG41900.021.1595823
GAGTACT41650.020.75390612-13
CAGAGTA61700.019.52687610-11
AGAGTAC58200.018.6546710-11
GTACTTT47750.018.47172214-15
GACCGCG1550.001290552117.6013627
Produced by FastQC (version 0.11.5)