##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931045_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2518804 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.4875135183206 30.0 2.0 31.0 2.0 33.0 2 20.167454871438984 28.0 2.0 31.0 2.0 33.0 3 20.857489109910894 28.0 2.0 31.0 2.0 34.0 4 22.390231633743635 32.0 2.0 35.0 2.0 37.0 5 21.759674829800176 32.0 2.0 35.0 2.0 37.0 6 22.739254820938825 32.0 2.0 35.0 2.0 37.0 7 22.932233313906124 32.0 2.0 35.0 2.0 37.0 8 23.382132948812213 32.0 2.0 35.0 2.0 37.0 9 24.739743941966108 32.0 2.0 37.0 2.0 39.0 10-11 25.124957122507347 33.0 2.0 37.0 2.0 39.0 12-13 25.05437640245132 33.0 2.0 37.0 2.0 39.0 14-15 25.055569627489874 33.0 2.0 38.0 2.0 40.0 16-17 23.92214717778755 32.0 2.0 38.0 2.0 40.0 18-19 22.862742595295227 30.5 2.0 38.0 2.0 40.0 20-21 22.53467062145367 30.5 2.0 38.0 2.0 40.0 22-23 22.185145013268205 29.0 2.0 38.0 2.0 40.0 24-25 22.03070862202855 29.0 2.0 38.0 2.0 40.0 26-27 21.75148999286963 28.0 2.0 37.5 2.0 40.0 28-29 21.5625314633453 27.0 2.0 37.0 2.0 40.0 30-31 21.558019996792126 27.0 2.0 37.0 2.0 39.0 32-33 21.443541260058346 28.0 2.0 37.0 2.0 39.5 34-35 21.13080970174734 27.0 2.0 37.0 2.0 39.0 36-37 20.695861607334272 25.0 2.0 37.0 2.0 39.0 38-39 20.582907403672536 25.0 2.0 36.5 2.0 39.0 40-41 20.453220655517462 25.0 2.0 36.0 2.0 39.0 42-43 20.319002193104346 25.0 2.0 36.0 2.0 39.0 44-45 19.93719261204921 24.0 2.0 36.0 2.0 39.0 46-47 19.602919877846787 24.0 2.0 35.5 2.0 39.0 48-49 19.366736951346752 23.5 2.0 35.0 2.0 39.0 50-51 16.97241607524841 11.5 2.0 33.5 2.0 37.5 52-53 18.954387677643837 22.5 2.0 34.5 2.0 38.5 54-55 19.762135719968683 24.0 2.0 36.0 2.0 39.0 56-57 19.73887587124683 24.0 2.0 36.0 2.0 39.0 58-59 19.471594455146175 23.5 2.0 36.0 2.0 39.0 60-61 19.18254199215183 23.0 2.0 36.0 2.0 39.0 62-63 18.74721256596385 21.5 2.0 35.0 2.0 39.0 64-65 18.396095329370603 19.5 2.0 35.0 2.0 38.5 66-67 18.029002852147286 18.0 2.0 34.0 2.0 38.0 68-69 17.58755067881423 14.0 2.0 34.0 2.0 38.0 70-71 17.22739006290287 12.0 2.0 34.0 2.0 37.5 72-73 16.825691280464856 8.0 2.0 33.0 2.0 37.0 74-75 16.373491148973876 7.0 2.0 32.5 2.0 36.0 76-77 15.951814829577849 4.0 2.0 32.0 2.0 36.0 78-79 15.578834438884487 2.0 2.0 31.5 2.0 35.0 80-81 15.141424461768363 2.0 2.0 31.0 2.0 35.0 82-83 14.676317609468622 2.0 2.0 31.0 2.0 34.5 84-85 14.279089599667143 2.0 2.0 31.0 2.0 34.0 86-87 13.792320283753718 2.0 2.0 30.0 2.0 34.0 88-89 13.372021800822932 2.0 2.0 30.0 2.0 34.0 90-91 12.879202986814377 2.0 2.0 29.0 2.0 34.0 92-93 12.329173488687488 2.0 2.0 29.0 2.0 34.0 94-95 11.751168014660927 2.0 2.0 28.5 2.0 34.0 96-97 11.092953044381382 2.0 2.0 26.5 2.0 34.0 98-99 10.39580034810172 2.0 2.0 25.0 2.0 33.5 100-101 9.398659443132534 2.0 2.0 21.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 8.068681875015717 1101 2 9.778074364487434 1101 3 8.988738649168592 1101 4 11.09826060902667 1101 5 11.85159377404328 1101 6 11.072688011183526 1101 7 10.775387902506573 1101 8 10.157738053657347 1101 9 10.276372769516556 1101 10-11 9.53287654669074 1101 12-13 9.518105877671545 1101 14-15 10.131525209394095 1101 16-17 11.009492932307626 1101 18-19 12.066636591102895 1101 20-21 12.101504559540853 1101 22-23 12.350754657104257 1101 24-25 12.292117380050104 1101 26-27 12.387143337332546 1101 28-29 12.315309052457792 1101 30-31 12.055431315438955 1101 32-33 12.025289806261505 1101 34-35 11.901783095765335 1101 36-37 12.407007198730344 1101 38-39 12.076218206563777 1101 40-41 11.818386295020584 1101 42-43 11.438202358978263 1101 44-45 11.31166807937679 1101 46-47 11.176591772039462 1101 48-49 11.415913482492545 1101 50-51 13.145180924013612 1101 52-53 12.154130213028317 1101 54-55 11.890800751515169 1101 56-57 11.78444575016422 1101 58-59 11.562820844581832 1101 60-61 11.41095535477659 1101 62-63 11.374531355225894 1101 64-65 11.33892064288938 1101 66-67 11.105001685937058 1101 68-69 11.097487111198447 1101 70-71 10.893524144804054 1101 72-73 10.738036046873411 1101 74-75 10.532229753510123 1101 76-77 10.482800316498414 1101 78-79 9.872354822518853 1101 80-81 9.675935858121791 1101 82-83 9.419583987696619 1101 84-85 9.254108722929265 1101 86-87 9.25143844756875 1101 88-89 9.155517967748304 1101 90-91 8.767118946706047 1101 92-93 8.569811089435122 1101 94-95 8.270369981096145 1101 96-97 8.144590489263448 1101 98-99 7.78075751809979 1101 100-101 6.915674260496122 1102 1 8.002733481223558 1102 2 9.218949877483333 1102 3 8.848082957862399 1102 4 10.672583926164268 1102 5 11.611651009447463 1102 6 10.689845424269048 1102 7 10.477597440692335 1102 8 9.811009434382854 1102 9 9.667876456797401 1102 10-11 8.948857646428149 1102 12-13 8.996037698354911 1102 14-15 9.666674194409108 1102 16-17 10.409014607858364 1102 18-19 11.4461823243922 1102 20-21 11.62695403042785 1102 22-23 11.829903359087059 1102 24-25 11.403665723525425 1102 26-27 11.656448021515764 1102 28-29 11.57714514535526 1102 30-31 11.46135187772963 1102 32-33 11.325519219598611 1102 34-35 11.224966107542624 1102 36-37 11.454148258843244 1102 38-39 11.319967597258309 1102 40-41 10.92319957237643 1102 42-43 10.670107323048892 1102 44-45 10.638767942577882 1102 46-47 10.479927149494376 1102 48-49 10.098783900908508 1102 50-51 12.16608521195172 1102 52-53 11.33472033520389 1102 54-55 10.908143550703812 1102 56-57 10.870913979797695 1102 58-59 10.863463956678313 1102 60-61 10.548336835035094 1102 62-63 10.513182392224493 1102 64-65 10.35193839529909 1102 66-67 10.282422004505545 1102 68-69 9.961671899789103 1102 70-71 9.696661242465893 1102 72-73 9.738991280610847 1102 74-75 9.414493382297646 1102 76-77 9.161742090481466 1102 78-79 8.877873164579013 1102 80-81 8.664831403509153 1102 82-83 8.701787079037409 1102 84-85 8.435293212763687 1102 86-87 8.182506422473226 1102 88-89 7.999892545165478 1102 90-91 8.017574156857469 1102 92-93 7.6894179891316465 1102 94-95 7.252367559598277 1102 96-97 7.024655429433581 1102 98-99 6.7400131433651325 1102 100-101 6.055280845712405 1103 1 8.136252556952247 1103 2 9.574175088223619 1103 3 8.824077629061609 1103 4 11.065719879491322 1103 5 11.583504988055694 1103 6 11.024111450867114 1103 7 10.621789277520783 1103 8 9.87513649871893 1103 9 9.959207355420034 1103 10-11 9.263205595682042 1103 12-13 9.01132247528794 1103 14-15 9.829625170973106 1103 16-17 10.85254585714058 1103 18-19 11.920494518617797 1103 20-21 12.018114575864292 1103 22-23 12.320936197056295 1103 24-25 12.343945868383056 1103 26-27 12.23873733864832 1103 28-29 11.913563058088485 1103 30-31 11.52004494339791 1103 32-33 11.709604633537715 1103 34-35 11.672452460146918 1103 36-37 11.945082100556423 1103 38-39 11.58564590919222 1103 40-41 11.22179211052406 1103 42-43 11.046293249196001 1103 44-45 10.963412038404417 1103 46-47 10.846097609869073 1103 48-49 10.754445522058337 1103 50-51 12.572378709436617 1103 52-53 11.782430558691956 1103 54-55 11.258915308059624 1103 56-57 10.87263045084531 1103 58-59 10.70495893030228 1103 60-61 10.692075494106689 1103 62-63 10.751079506556366 1103 64-65 10.768646635799506 1103 66-67 10.572859257110611 1103 68-69 10.490846088793091 1103 70-71 10.207062893169123 1103 72-73 10.162630943426386 1103 74-75 9.956190406284904 1103 76-77 9.827784154319884 1103 78-79 9.508680676963115 1103 80-81 9.118043460686573 1103 82-83 8.8387624486464 1103 84-85 8.658595461425746 1103 86-87 8.490382941296298 1103 88-89 8.393335899859954 1103 90-91 8.037787997958143 1103 92-93 7.5763852430382865 1103 94-95 7.185785878116626 1103 96-97 6.820112014254159 1103 98-99 6.273504656331113 1103 100-101 5.747002599622871 1104 1 7.946192891859212 1104 2 9.464500289586752 1104 3 8.857146975023966 1104 4 10.86034050288325 1104 5 11.56312517300924 1104 6 10.891870940809635 1104 7 10.520352323835617 1104 8 10.148493909938907 1104 9 9.788602812598846 1104 10-11 8.979654379708794 1104 12-13 8.850825248691734 1104 14-15 9.453271988997198 1104 16-17 10.567908138399172 1104 18-19 11.575574895240113 1104 20-21 11.784271030164401 1104 22-23 11.926515831331233 1104 24-25 11.620858527710435 1104 26-27 11.797797236825687 1104 28-29 11.655199415047782 1104 30-31 11.48805617941191 1104 32-33 11.466292242819414 1104 34-35 11.322303053837455 1104 36-37 11.422634954865227 1104 38-39 10.949722216956474 1104 40-41 10.719160206150097 1104 42-43 10.448057720098308 1104 44-45 10.679868766784725 1104 46-47 10.296170045885177 1104 48-49 10.25166185055286 1104 50-51 12.020821578667313 1104 52-53 11.249280570046388 1104 54-55 10.774797045115012 1104 56-57 10.517547151300459 1104 58-59 10.666090880768486 1104 60-61 10.407866020989708 1104 62-63 10.25532156651225 1104 64-65 10.149039746396152 1104 66-67 10.098755601283987 1104 68-69 9.88089473671658 1104 70-71 9.697887014924586 1104 72-73 9.425634613567833 1104 74-75 9.326366853375134 1104 76-77 8.974858646124835 1104 78-79 8.76635308629818 1104 80-81 8.515841150978199 1104 82-83 8.233253339245906 1104 84-85 7.969798124133229 1104 86-87 7.720264128242677 1104 88-89 7.417759016130061 1104 90-91 7.129604189599933 1104 92-93 6.661439365820518 1104 94-95 6.6008979122383735 1104 96-97 6.091189620845272 1104 98-99 5.654162403837075 1104 100-101 4.963341137007426 1105 1 7.95415365882922 1105 2 9.112357992148038 1105 3 8.66122844761335 1105 4 10.786413576071904 1105 5 10.713923827768888 1105 6 10.503319862852653 1105 7 10.095407861114765 1105 8 9.77559290681836 1105 9 9.729313326664531 1105 10-11 9.19404025976861 1105 12-13 8.778616764033387 1105 14-15 9.567510810553866 1105 16-17 10.517798298406088 1105 18-19 10.994349291519573 1105 20-21 11.512651433111966 1105 22-23 11.817375037883433 1105 24-25 11.72717354663197 1105 26-27 11.875933232172102 1105 28-29 11.52889293641266 1105 30-31 11.160939245026146 1105 32-33 11.158070909968295 1105 34-35 11.327266078609366 1105 36-37 11.192348165366582 1105 38-39 10.899126974970802 1105 40-41 10.901708174455177 1105 42-43 10.428657544499561 1105 44-45 10.436878156016267 1105 46-47 10.257312251094607 1105 48-49 10.167400668841562 1105 50-51 11.665281757766412 1105 52-53 10.812018414481003 1105 54-55 10.602889939611249 1105 56-57 10.35806071590417 1105 58-59 10.380434422503686 1105 60-61 10.08813897609583 1105 62-63 10.230606442434816 1105 64-65 9.850653758856541 1105 66-67 9.699434757393309 1105 68-69 9.468000937647155 1105 70-71 9.278486040489526 1105 72-73 9.061508028513188 1105 74-75 8.912398837328691 1105 76-77 8.581882305477789 1105 78-79 8.378004477035892 1105 80-81 8.012555246735433 1105 82-83 7.941127178300725 1105 84-85 7.796988478838339 1105 86-87 7.447129066082317 1105 88-89 7.057216664370484 1105 90-91 6.69620694936153 1105 92-93 6.35404330350257 1105 94-95 6.187016243135254 1105 96-97 5.748876568513504 1105 98-99 5.560216566391549 1105 100-101 4.998301699911066 1106 1 8.09109679653092 1106 2 9.481516923063289 1106 3 8.998622633394902 1106 4 11.01723462696992 1106 5 11.497633424927727 1106 6 10.776946185774403 1106 7 9.908525594317766 1106 8 9.564049937638565 1106 9 9.375784340401125 1106 10-11 8.910999426216986 1106 12-13 8.805097772666059 1106 14-15 9.528792797948082 1106 16-17 10.620565209337226 1106 18-19 11.721128798291321 1106 20-21 11.655255174525262 1106 22-23 11.733827502853384 1106 24-25 11.596403464024998 1106 26-27 11.762671396419194 1106 28-29 11.692568019945995 1106 30-31 11.438700956197781 1106 32-33 11.659185113723616 1106 34-35 11.28609328261528 1106 36-37 11.630367954976858 1106 38-39 11.25374716537073 1106 40-41 10.935615788393793 1106 42-43 10.912573570335802 1106 44-45 10.571875767321288 1106 46-47 10.217695975598925 1106 48-49 10.25231220904227 1106 50-51 12.241474241860121 1106 52-53 11.470431625348464 1106 54-55 11.078075125585173 1106 56-57 10.939326958497617 1106 58-59 10.55895746364958 1106 60-61 10.341742640133866 1106 62-63 10.568214581460413 1106 64-65 10.450994422963436 1106 66-67 10.374836381138 1106 68-69 9.870361521979444 1106 70-71 9.663468070262674 1106 72-73 9.449045358527194 1106 74-75 9.027827819261923 1106 76-77 8.766957114577407 1106 78-79 8.399577906513313 1106 80-81 8.170229630342101 1106 82-83 8.081210615806711 1106 84-85 7.7869685245394304 1106 86-87 7.632353017771761 1106 88-89 7.352322004754191 1106 90-91 7.078610466315586 1106 92-93 6.809174454569602 1106 94-95 6.60367904103888 1106 96-97 5.92519768010496 1106 98-99 5.347270620464789 1106 100-101 4.722974257035657 1107 1 7.37085984287933 1107 2 8.322915019095532 1107 3 8.12361672692558 1107 4 9.94868414402919 1107 5 10.814940769796046 1107 6 9.795074182438984 1107 7 9.795418791454864 1107 8 8.089811177302241 1107 9 5.839989746557752 1107 10-11 7.769489454707024 1107 12-13 7.735503929291724 1107 14-15 8.343395327306713 1107 16-17 9.426944891082758 1107 18-19 10.699112607616602 1107 20-21 10.910298257007366 1107 22-23 10.361646278477036 1107 24-25 10.58334981938599 1107 26-27 10.572994862474367 1107 28-29 10.768321615943492 1107 30-31 10.524380200169713 1107 32-33 10.801172814369856 1107 34-35 10.829170900570944 1107 36-37 11.117034969082898 1107 38-39 10.91479330531931 1107 40-41 10.318210465622617 1107 42-43 10.037710808887677 1107 44-45 9.450401929505986 1107 46-47 9.908946757337514 1107 48-49 10.020548606790861 1107 50-51 11.427588479925578 1107 52-53 10.477999202306286 1107 54-55 10.221694748960893 1107 56-57 10.207340995048238 1107 58-59 10.09665567781121 1107 60-61 9.885515484272567 1107 62-63 10.020764091570896 1107 64-65 10.039934038000613 1107 66-67 9.878565795355218 1107 68-69 9.771748152512416 1107 70-71 9.471890000635824 1107 72-73 9.152684091068366 1107 74-75 9.370517347439257 1107 76-77 9.117973027300634 1107 78-79 8.555607369372042 1107 80-81 8.222151664947061 1107 82-83 8.262985336118021 1107 84-85 8.162264890323604 1107 86-87 7.664619712233575 1107 88-89 7.399042871962861 1107 90-91 7.121484832238734 1107 92-93 6.522598817018357 1107 94-95 6.276833790535093 1107 96-97 5.771343882592626 1107 98-99 5.521941247940294 1107 100-101 5.244452959002166 1108 1 6.680539522559009 1108 2 7.79622332740384 1108 3 7.873081191390046 1108 4 9.299925385270434 1108 5 10.969440269295546 1108 6 9.286395503760303 1108 7 8.797976349012423 1108 8 5.898750116241182 1108 9 6.903483240051248 1108 10-11 6.08845091616848 1108 12-13 4.901970395758191 1108 14-15 7.25243686884826 1108 16-17 8.174890336528204 1108 18-19 7.971037032041021 1108 20-21 8.14582128003039 1108 22-23 8.94894858077934 1108 24-25 8.820794764330937 1108 26-27 7.253728095707601 1108 28-29 8.946294588916466 1108 30-31 9.450028348317861 1108 32-33 9.702831973529015 1108 34-35 9.255727457127502 1108 36-37 8.96858652063445 1108 38-39 8.764645657671661 1108 40-41 9.047134389546542 1108 42-43 8.355491089167955 1108 44-45 7.656790818394878 1108 46-47 8.540343153733907 1108 48-49 9.130060618802876 1108 50-51 10.959014906352003 1108 52-53 10.066029732836817 1108 54-55 9.567395449661593 1108 56-57 9.407005659712905 1108 58-59 9.398947970103503 1108 60-61 9.283836305093388 1108 62-63 9.384855683162492 1108 64-65 8.953652756719332 1108 66-67 8.580224954514375 1108 68-69 8.726107511871774 1108 70-71 8.782513123758946 1108 72-73 8.499545952930227 1108 74-75 8.376243073164982 1108 76-77 7.9734109652385765 1108 78-79 7.922006268270939 1108 80-81 7.823225238520637 1108 82-83 7.794436787569467 1108 84-85 7.510265390824101 1108 86-87 7.025830923444788 1108 88-89 6.772188517608511 1108 90-91 6.489745592999492 1108 92-93 6.186402620822159 1108 94-95 6.040492449193756 1108 96-97 5.4435310697798105 1108 98-99 5.2622790857781325 1108 100-101 4.731039545588752 1109 1 6.423722347709049 1109 2 7.966919545231203 1109 3 7.802052450279337 1109 4 8.807221429451726 1109 5 9.975287957520283 1109 6 8.954186618272729 1109 7 7.396439379852502 1109 8 8.174264453230926 1109 9 7.460356805121535 1109 10-11 5.59006626102153 1109 12-13 6.111802433459083 1109 14-15 7.501233821538651 1109 16-17 6.421153645291515 1109 18-19 5.625616443792563 1109 20-21 8.074594222123004 1109 22-23 9.114018083963597 1109 24-25 8.5568021017727 1109 26-27 9.115008992849155 1109 28-29 9.622849683504349 1109 30-31 6.969495561432616 1109 32-33 5.862341054144654 1109 34-35 7.715861025867628 1109 36-37 6.125050920951306 1109 38-39 6.454701678670304 1109 40-41 7.497975879322457 1109 42-43 6.299602954386376 1109 44-45 6.891525459891813 1109 46-47 6.202752023109991 1109 48-49 7.04437125954794 1109 50-51 8.95159410999678 1109 52-53 9.29781843286322 1109 54-55 9.511034319529973 1109 56-57 8.611821317397414 1109 58-59 8.302638732400823 1109 60-61 8.610925596322023 1109 62-63 9.139523820002758 1109 64-65 8.958612075203241 1109 66-67 8.498365431056495 1109 68-69 8.285063822877266 1109 70-71 7.679084900453674 1109 72-73 7.1337927183245355 1109 74-75 6.205403642079652 1109 76-77 6.1695770456259655 1109 78-79 6.957240366005037 1109 80-81 7.225547922605616 1109 82-83 7.006450589747207 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2313 56-57 -17.456733079025835 2313 58-59 -17.175751729596197 2313 60-61 -16.89578081202373 2313 62-63 -16.463680352873546 2313 64-65 -16.130806702740127 2313 66-67 -15.78032309353367 2313 68-69 -15.336955551191288 2313 70-71 -14.972554443808619 2313 72-73 -14.584538131153856 2313 74-75 -14.150408578486669 2313 76-77 -13.726101046773438 2313 78-79 -13.349577815813143 2313 80-81 -12.91007055727516 2313 82-83 -12.453310960178278 2313 84-85 -12.075883257824545 2313 86-87 -11.592787695173831 2313 88-89 -11.172068239148247 2313 90-91 -10.68536701961312 2313 92-93 -10.126660442240903 2313 94-95 -9.567240283538979 2313 96-97 -8.914952413703672 2313 98-99 -8.235280974281928 2313 100-101 -7.262954448405242 2314 1 -19.184325342305854 2314 2 -17.896344240163728 2314 3 -18.558975865667012 2314 4 -20.070945485600436 2314 5 -19.395799970944694 2314 6 -20.396036928672128 2314 7 -20.588284912248838 2314 8 -21.077225859734796 2314 9 -22.41408432751632 2314 10-11 -22.81976980455224 2314 12-13 -22.76357014478235 2314 14-15 -22.759012080100693 2314 16-17 -21.60157362743576 2314 18-19 -20.53440591090192 2314 20-21 -20.21284989114078 2314 22-23 -19.848724091893335 2314 24-25 -19.68202055098438 2314 26-27 -19.412375507896 2314 28-29 -19.23501171738984 2314 30-31 -19.23747165168214 2314 32-33 -19.116049407852366 2314 34-35 -18.819347617947574 2314 36-37 -18.38389095684303 2314 38-39 -18.27983632431032 2314 40-41 -18.14993768252553 2314 42-43 -18.019696598519733 2314 44-45 -17.641820292716236 2314 46-47 -17.314386575995822 2314 48-49 -17.06945139320914 2314 50-51 -14.786855964518868 2314 52-53 -16.70292672361964 2314 54-55 -17.47126821400207 2314 56-57 -17.456733079025835 2314 58-59 -17.175751729596197 2314 60-61 -16.89578081202373 2314 62-63 -16.463680352873546 2314 64-65 -16.130806702740127 2314 66-67 -15.78032309353367 2314 68-69 -15.336955551191288 2314 70-71 -14.972554443808619 2314 72-73 -14.584538131153856 2314 74-75 -14.150408578486669 2314 76-77 -13.726101046773438 2314 78-79 -13.349577815813143 2314 80-81 -12.91007055727516 2314 82-83 -12.453310960178278 2314 84-85 -12.075883257824545 2314 86-87 -11.592787695173831 2314 88-89 -11.172068239148247 2314 90-91 -10.68536701961312 2314 92-93 -10.126660442240903 2314 94-95 -9.567240283538979 2314 96-97 -8.914952413703672 2314 98-99 -8.235280974281928 2314 100-101 -7.262954448405242 2315 1 -19.184325342305854 2315 2 -17.896344240163728 2315 3 -18.558975865667012 2315 4 -20.070945485600436 2315 5 -19.395799970944694 2315 6 -20.396036928672128 2315 7 -20.588284912248838 2315 8 -21.077225859734796 2315 9 -22.41408432751632 2315 10-11 -22.81976980455224 2315 12-13 -22.76357014478235 2315 14-15 -22.759012080100693 2315 16-17 -21.60157362743576 2315 18-19 -20.53440591090192 2315 20-21 -20.21284989114078 2315 22-23 -19.848724091893335 2315 24-25 -19.68202055098438 2315 26-27 -19.412375507896 2315 28-29 -19.23501171738984 2315 30-31 -19.23747165168214 2315 32-33 -19.116049407852366 2315 34-35 -18.819347617947574 2315 36-37 -18.38389095684303 2315 38-39 -18.27983632431032 2315 40-41 -18.14993768252553 2315 42-43 -18.019696598519733 2315 44-45 -17.641820292716236 2315 46-47 -17.314386575995822 2315 48-49 -17.06945139320914 2315 50-51 -14.786855964518868 2315 52-53 -16.70292672361964 2315 54-55 -17.47126821400207 2315 56-57 -17.456733079025835 2315 58-59 -17.175751729596197 2315 60-61 -16.89578081202373 2315 62-63 -16.463680352873546 2315 64-65 -16.130806702740127 2315 66-67 -15.78032309353367 2315 68-69 -15.336955551191288 2315 70-71 -14.972554443808619 2315 72-73 -14.584538131153856 2315 74-75 -14.150408578486669 2315 76-77 -13.726101046773438 2315 78-79 -13.349577815813143 2315 80-81 -12.91007055727516 2315 82-83 -12.453310960178278 2315 84-85 -12.075883257824545 2315 86-87 -11.592787695173831 2315 88-89 -11.172068239148247 2315 90-91 -10.68536701961312 2315 92-93 -10.126660442240903 2315 94-95 -9.567240283538979 2315 96-97 -8.914952413703672 2315 98-99 -8.235280974281928 2315 100-101 -7.262954448405242 2316 1 -19.184325342305854 2316 2 -17.896344240163728 2316 3 -18.558975865667012 2316 4 -20.070945485600436 2316 5 -19.395799970944694 2316 6 -20.396036928672128 2316 7 -20.588284912248838 2316 8 -21.077225859734796 2316 9 -22.41408432751632 2316 10-11 -22.81976980455224 2316 12-13 -22.76357014478235 2316 14-15 -22.759012080100693 2316 16-17 -21.60157362743576 2316 18-19 -20.53440591090192 2316 20-21 -20.21284989114078 2316 22-23 -19.848724091893335 2316 24-25 -19.68202055098438 2316 26-27 -19.412375507896 2316 28-29 -19.23501171738984 2316 30-31 -19.23747165168214 2316 32-33 -19.116049407852366 2316 34-35 -18.819347617947574 2316 36-37 -18.38389095684303 2316 38-39 -18.27983632431032 2316 40-41 -18.14993768252553 2316 42-43 -18.019696598519733 2316 44-45 -17.641820292716236 2316 46-47 -17.314386575995822 2316 48-49 -17.06945139320914 2316 50-51 -14.786855964518868 2316 52-53 -16.70292672361964 2316 54-55 -17.47126821400207 2316 56-57 -17.456733079025835 2316 58-59 -17.175751729596197 2316 60-61 -16.89578081202373 2316 62-63 -16.463680352873546 2316 64-65 -16.130806702740127 2316 66-67 -15.78032309353367 2316 68-69 -15.336955551191288 2316 70-71 -14.972554443808619 2316 72-73 -14.584538131153856 2316 74-75 -14.150408578486669 2316 76-77 -13.726101046773438 2316 78-79 -13.349577815813143 2316 80-81 -12.91007055727516 2316 82-83 -12.453310960178278 2316 84-85 -12.075883257824545 2316 86-87 -11.592787695173831 2316 88-89 -11.172068239148247 2316 90-91 -10.68536701961312 2316 92-93 -10.126660442240903 2316 94-95 -9.567240283538979 2316 96-97 -8.914952413703672 2316 98-99 -8.235280974281928 2316 100-101 -7.262954448405242 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 639455.0 3 23144.0 4 95093.0 5 46822.0 6 26633.0 7 26764.0 8 25365.0 9 24355.0 10 26336.0 11 28219.0 12 29985.0 13 30756.0 14 31766.0 15 33104.0 16 34594.0 17 35212.0 18 34744.0 19 35313.0 20 37702.0 21 39541.0 22 42677.0 23 45780.0 24 49836.0 25 53680.0 26 57472.0 27 62998.0 28 68361.0 29 74675.0 30 81603.0 31 89974.0 32 99162.0 33 108244.0 34 115839.0 35 115873.0 36 94092.0 37 46181.0 38 7379.0 39 75.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.07710790347055 20.452913616323315 14.14579724311226 23.32418123709388 2 14.810203249927387 20.715247459657355 42.064314068083206 22.41023522233205 3 18.44458328850785 26.26542341462607 30.324646844148003 24.96534645271808 4 12.096213150521207 17.3873162811413 39.9309380584856 30.585532509851898 5 12.625232080388935 37.9839365901285 35.961721687370996 13.429109642111564 6 29.402054623434186 38.817448886857534 18.283438119482785 13.497058370225497 7 25.835865146688004 33.06487991110231 23.469660938717283 17.62959400349241 8 24.017317077297786 37.085499461018664 20.941689485036424 17.955493976647126 9 25.03762285809177 17.074031406005126 21.69549468812625 36.192851047776855 10-11 23.584803066089364 27.47218990847586 29.35290487110307 19.59010215433171 12-13 24.28441728251877 24.982321472795096 29.634136370073488 21.099124874612645 14-15 21.596020153144657 26.73921792131208 27.170072099142082 24.494689826401174 16-17 20.951780411209114 29.92413524472927 27.829189981051915 21.294894363009696 18-19 20.72470842320834 28.711553721847878 29.99828203527009 20.565455819673694 20-21 21.535096842543176 27.83917487688532 30.012359891520408 20.613368389051097 22-23 21.137531502452113 27.815158455380924 29.724530727865687 21.322779314301275 24-25 21.01430479598918 28.08514383375748 29.92317222797259 20.977379142280746 26-27 20.979866131310946 28.24407824109309 29.698572260747103 21.077483366848863 28-29 21.012603117203614 28.265085057067967 29.78670146093681 20.935610364791607 30-31 21.24870852353883 27.95420523944968 29.757072077078334 21.04001415993315 32-33 21.116469895149045 28.13001539951007 29.652720471818462 21.100794233522414 34-35 21.119683950262996 28.20566654938066 29.681518380756795 20.993131119599546 36-37 21.183378475651974 28.13248616218181 29.577955467509888 21.106179894656332 38-39 21.142852270389273 28.129535029821863 29.720941976923676 21.006670722865188 40-41 21.27891732448369 28.154462862704467 29.618163545586846 20.948456267225 42-43 21.25509460025831 28.19313292790365 29.361790391145554 21.18998208069248 44-45 21.145004050319073 28.496930927542774 29.247691659705932 21.11037336243222 46-47 21.271305482132437 28.503710823521335 28.953238817754283 21.271744876591946 48-49 21.39990769192645 28.48486209545522 28.877093968379842 21.23813624423849 50-51 21.494149710708854 28.68088417243128 28.579408797335798 21.24555731952407 52-53 20.989376857721815 28.848060511608736 28.77519465374543 21.387367976924025 54-55 21.059914727132785 28.83897053505393 28.711426756040638 21.389687981772646 56-57 21.07514032923983 28.966290589102865 28.73290174793685 21.22566733372045 58-59 21.069737049233673 28.885573258871645 28.855489595119842 21.18920009677484 60-61 20.989360688938092 28.94831363863074 28.85921324562874 21.203112426802427 62-63 20.963530243324634 28.865996120444816 28.799398922974305 21.371074713256245 64-65 20.81275482561457 28.776865308727068 28.87194283120362 21.53843703445475 66-67 20.84875867982825 28.89370397609317 28.57001736741443 21.687519976664152 68-69 20.91217700345548 28.862421894176087 28.596351931161827 21.629049171206603 70-71 20.97694779650812 28.585893326504348 28.673738642717943 21.76342023426959 72-73 20.89089401656025 28.782341271977657 28.68787022523846 21.638894486223634 74-75 20.864910633134716 28.9334066920053 28.41068899597626 21.790993678883726 76-77 20.904533653038666 28.927852976325646 28.42844082737008 21.73917254326561 78-79 20.695913480917756 28.96675118105158 28.529575667918934 21.807759670111736 80-81 20.784492984380368 28.98517711742241 28.268024888244227 21.962305009953 82-83 20.943297969599634 28.759129778990218 28.468438183950802 21.82913406745935 84-85 21.06289303646723 28.84016945377011 28.23726209831527 21.859675411447395 86-87 20.79991774103927 28.998056480096523 28.28885153017369 21.913174248690517 88-89 20.88218986385965 28.842175710333823 28.33672116306449 21.93891326274204 90-91 20.876273389681423 28.879518359042084 28.355173239738317 21.889035011538173 92-93 20.821368033714805 28.751323946057934 28.182710631464047 22.244597388763214 94-95 20.667191303764877 28.763203895055238 28.29200607151541 22.277598729664476 96-97 20.69296995368952 28.74323312963037 28.24305330466001 22.320743612020102 98-99 20.55224665359207 28.721094976202345 28.05621945738795 22.67043891281764 100-101 20.75114992593867 28.79365542928068 27.971004563698248 22.484190081082406 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 153253.0 1 139344.5 2 92664.0 3 46908.5 4 28937.0 5 22424.0 6 20712.5 7 21121.5 8 22001.5 9 21742.5 10 20020.5 11 17094.5 12 13715.5 13 10492.0 14 8006.0 15 6519.0 16 5912.5 17 6354.5 18 7303.0 19 8605.0 20 10144.0 21 11552.5 22 13232.5 23 15258.5 24 17325.5 25 19440.5 26 22044.0 27 25021.5 28 28413.0 29 32700.0 30 36875.5 31 41092.0 32 45531.5 33 49360.0 34 52899.5 35 56678.5 36 61507.0 37 65962.0 38 68543.0 39 69991.0 40 71173.5 41 72211.5 42 73240.0 43 73968.5 44 74278.0 45 74459.0 46 74040.0 47 73122.5 48 71244.5 49 68412.5 50 64596.5 51 60408.0 52 56280.5 53 51211.5 54 45654.0 55 39472.0 56 32725.5 57 26345.5 58 20640.0 59 16019.5 60 12142.0 61 8896.5 62 6307.0 63 4308.5 64 2862.5 65 1790.0 66 1100.5 67 672.0 68 418.0 69 242.5 70 149.5 71 104.0 72 65.5 73 42.0 74 30.0 75 24.0 76 19.5 77 14.5 78 11.0 79 8.0 80 5.0 81 4.0 82 2.0 83 0.5 84 1.0 85 2.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.422903886130083 2 29.469224282635725 3 23.240395044632294 4 29.578879499953153 5 32.620839096650634 6 25.29748245595926 7 24.221535300086867 8 20.226186714011888 9 12.018839099826744 10-11 15.668745960384372 12-13 15.756664671010528 14-15 19.943770932553704 16-17 23.25020128600717 18-19 27.204935358209692 20-21 29.28530763012922 22-23 28.858140609590897 24-25 27.372217131622783 26-27 28.84702422260724 28-29 25.772052926706486 30-31 27.662394533278494 32-33 26.67901114973614 34-35 24.74003535011061 36-37 27.692785941264187 38-39 27.831065855064548 40-41 24.882861072159642 42-43 25.73427706165307 44-45 24.450790930933888 46-47 27.71618990600301 48-49 23.098740513354752 50-51 28.71579130412688 52-53 22.276326383474064 54-55 23.42597915518635 56-57 25.543591323501154 58-59 24.843139839384087 60-61 24.34046079012103 62-63 24.2005531196552 64-65 22.728941989928554 66-67 23.237139531301363 68-69 24.221634553542078 70-71 22.717150679449453 72-73 22.56908437496526 74-75 19.866988459602254 76-77 22.113570567618602 78-79 21.205778615565166 80-81 22.266579694172314 82-83 23.11476002102585 84-85 20.064820446529385 86-87 21.61942731550371 88-89 20.448712960595582 90-91 21.59973542998979 92-93 21.58357696748139 94-95 20.643210031427614 96-97 19.526449854772345 98-99 18.904448301654277 100-101 20.609384453891607 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2518804.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.92446512576093 #Duplication Level Percentage of deduplicated Percentage of total 1 87.28710796056582 65.39939876319943 2 8.394376466677056 12.578883336601068 3 2.164381481268993 4.864953744365445 4 0.8224144025994711 2.464758369059503 5 0.3966106650189248 1.4857920969857639 6 0.23717509288947994 1.0662130185538168 7 0.15558865904163477 0.8160177940830212 8 0.10944602427879245 0.6560147863383656 9 0.07587884855784059 0.511666392829934 >10 0.34096047677440033 4.257025502906124 >50 0.010963949011909137 0.5505765788772801 >100 0.0048286623604622695 0.5873320774790493 >500 1.0692438208963014E-4 0.05599865243711788 >1k 1.0692438208728099E-4 0.15609924788539253 >5k 0.0 0.0 >10k+ 5.346219104364049E-5 4.549269638398718 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 113572 4.508965366102325 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.970138208451313E-5 0.0 9 0.0 0.0 0.0 3.970138208451313E-5 0.0 10-11 0.0 0.0 0.0 5.95520731267697E-5 0.0 12-13 0.0 0.0 0.0 7.940276416902626E-5 0.0 14-15 3.970138208451313E-5 0.0 0.0 7.940276416902626E-5 0.0 16-17 3.970138208451313E-5 0.0 0.0 7.940276416902626E-5 0.0 18-19 3.970138208451313E-5 0.0 0.0 7.940276416902626E-5 0.0 20-21 3.970138208451313E-5 0.0 0.0 7.940276416902626E-5 0.0 22-23 3.970138208451313E-5 0.0 0.0 2.183576014648222E-4 3.970138208451313E-5 24-25 3.970138208451313E-5 0.0 0.0 3.1761105667610503E-4 7.940276416902626E-5 26-27 3.970138208451313E-5 0.0 0.0 3.7716312980287474E-4 7.940276416902626E-5 28-29 3.970138208451313E-5 0.0 0.0 4.56565893971901E-4 7.940276416902626E-5 30-31 3.970138208451313E-5 0.0 0.0 0.0013299962998311898 7.940276416902626E-5 32-33 3.970138208451313E-5 0.0 0.0 0.0033349160950991025 7.940276416902626E-5 34-35 3.970138208451313E-5 0.0 0.0 0.0071660994662546195 1.1910414625353938E-4 36-37 3.970138208451313E-5 0.0 0.0 0.012347129828283582 1.1910414625353938E-4 38-39 3.970138208451313E-5 0.0 0.0 0.021220388724172267 1.1910414625353938E-4 40-41 3.970138208451313E-5 0.0 0.0 0.030292154530483516 1.1910414625353938E-4 42-43 3.970138208451313E-5 0.0 0.0 0.040634364563499184 1.3895483729579595E-4 44-45 3.970138208451313E-5 0.0 0.0 0.05321970268428984 1.5880552833805252E-4 46-47 3.970138208451313E-5 0.0 0.0 0.06834592925848934 1.5880552833805252E-4 48-49 3.970138208451313E-5 0.0 0.0 0.08670781847257666 1.5880552833805252E-4 50-51 3.970138208451313E-5 0.0 0.0 0.1087222348384392 1.5880552833805252E-4 52-53 3.970138208451313E-5 0.0 0.0 0.1320269461220484 1.5880552833805252E-4 54-55 3.970138208451313E-5 0.0 0.0 0.15745568134717905 1.5880552833805252E-4 56-57 3.970138208451313E-5 0.0 0.0 0.19048723124149397 1.5880552833805252E-4 58-59 3.970138208451313E-5 0.0 0.0 0.22953354052161262 1.5880552833805252E-4 60-61 3.970138208451313E-5 0.0 0.0 0.2719147658968304 1.5880552833805252E-4 62-63 7.940276416902626E-5 0.0 0.0 0.31530837651520327 1.5880552833805252E-4 64-65 7.940276416902626E-5 0.0 0.0 0.360627504164675 1.5880552833805252E-4 66-67 7.940276416902626E-5 0.0 0.0 0.414482428962317 1.9850691042256564E-4 68-69 7.940276416902626E-5 0.0 0.0 0.4755431546082982 1.9850691042256564E-4 70-71 7.940276416902626E-5 0.0 0.0 0.5433531152086466 1.9850691042256564E-4 72-73 7.940276416902626E-5 0.0 0.0 0.6163838075531085 1.9850691042256564E-4 74-75 7.940276416902626E-5 0.0 0.0 0.6911812114003313 1.9850691042256564E-4 76-77 7.940276416902626E-5 0.0 0.0 0.7775515681251897 1.9850691042256564E-4 78-79 7.940276416902626E-5 0.0 0.0 0.8745618952486974 1.9850691042256564E-4 80-81 7.940276416902626E-5 0.0 0.0 0.9832841300871367 1.9850691042256564E-4 82-83 7.940276416902626E-5 0.0 0.0 1.0947854616714916 1.9850691042256564E-4 84-85 7.940276416902626E-5 0.0 0.0 1.2127184171535381 1.9850691042256564E-4 86-87 7.940276416902626E-5 0.0 0.0 1.338611499743529 1.9850691042256564E-4 88-89 7.940276416902626E-5 0.0 0.0 1.4792536457779168 1.9850691042256564E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1880 0.0 52.618645 1 GTATCAA 3805 0.0 46.20401 1 ATCAACG 4410 0.0 38.83728 3 TCAACGC 4410 0.0 38.83496 4 CAACGCA 4405 0.0 38.757736 5 TATCAAC 4635 0.0 37.59154 2 AACGCAG 4745 0.0 34.830185 6 ACGCAGA 5675 0.0 28.7644 7 CGCAGAG 5725 0.0 28.063707 8 GTACATG 3935 0.0 24.357746 1 GTGGTAT 1160 0.0 23.418447 1 GCAGAGT 6625 0.0 23.042858 9 TGGTATC 1080 0.0 22.301548 2 TACATGG 4225 0.0 21.711372 2 ACATGGG 4190 0.0 21.159582 3 GAGTACT 4165 0.0 20.753906 12-13 CAGAGTA 6170 0.0 19.526876 10-11 AGAGTAC 5820 0.0 18.65467 10-11 GTACTTT 4775 0.0 18.471722 14-15 GACCGCG 155 0.0012905521 17.601362 7 >>END_MODULE