FastQCFastQC Report
Sat 18 Jun 2016
SRR1931045_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931045_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518804
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63310.2513494499770526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56660.22494803089085136No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30920.12275667340531457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35650.054.918491
GTATCAA65900.041.2469181
ATCAACG80100.033.5174453
TCAACGC80600.033.3095254
CAACGCA80550.033.2203525
TATCAAC83900.031.8861032
AACGCAG84600.031.7969976
ACGCAGA96500.027.7284247
CGCAGAG97650.027.3532898
GTGGTAT18550.025.1051651
GCAGAGT112850.023.2481469
GTACATG62500.022.0495071
TGGTATC19250.021.7223552
ACATGGG59950.021.480043
TACATGG62650.021.1610952
GAGTACT80650.020.94162612-13
AGAGTAC102100.020.67754710-11
CAGAGTA107600.020.45834410-11
ACCGGGC1751.00345685E-418.9772558
AGTACTT87400.018.83574712-13
Produced by FastQC (version 0.11.5)